NS-398 (NS398)

Catalog No.S8433 Synonyms: N-(2-cyclohexyloxy-4-nitrophenyl)methane sulfonamide

NS-398 (NS398) Chemical Structure

Molecular Weight(MW): 314.36

NS-398 is a selective inhibitor of cyclooxygenase-2 (COX-2). The IC50 values for human recombinant COX-1 and -2 are 75 and 1.77 μM, respectively.

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  • (C)Percentage of TUNEL-positive cell in B were calculated. Data are mean ± SEM; ** P < 0.01 versus the Sham group; # P < 0.05 versus the Vehicle group; unpaired two-tailed Student’s t-test. n = 5 per group. (D-G) ELISA of cytokines IL-1α (D), IL-1β (E), IL-6 (F), and TNF-α (G) in tSCI rats with or without NS-398 treatment. Data are mean ± SEM; * P < 0.05 or ** P < 0.01 versus the Sham group; # P <0.05 versus the Vehicle group; unpaired two-tailed Student’s t-test. n = 5 per group. (H) The mean CBS score in tSCI rats was improved by NS-398 treatment. Data are mean ± SEM; ** P < 0.05 versus the Sham group; # P < 0.05 versus the Vehicle group; unpaired two-tailed Student’s t-test. n = 5 per group.

    Oncotarget, 2017, 8(50): 87658-87666. NS-398 (NS398) purchased from Selleck.

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Biological Activity

Description NS-398 is a selective inhibitor of cyclooxygenase-2 (COX-2). The IC50 values for human recombinant COX-1 and -2 are 75 and 1.77 μM, respectively.
COX-2 [1]
(Cell-free assay)
3.8 μM
In vitro

NS-398 inhibits COX-2 enzyme activity in a concentration dependent manner, the IC50 being 3.8 μM, whereas NS-398 at 100μM has no effect on COX-1 activity[1]. At 10 μM, NS-398 treatment results in increased production of COX-2 and the pro-inflammatory cytokine. NS-398 (10 μM) induces apoptosis in LNCaP cells, but not in the more aggressive, androgen-unresponsive C4-2b cells. The C4-2b cells are observed to continue to proliferate when treated with NS-398 and continues to retain malignant phenotype characteristics. NS-398 treatment results in C4-2b cell differentiation into an unusual neuroendocrinelike cell. These neuroendocrine-like cells produces both epithelial (cytokeratin 18 and prostate specific antigen) and neuronal (neuron-specific enolase and chromogranin A) proteins. Furthermore, this C4-2b cellular response to NS-398 is mediated by NF-kB transcription factor activation. NS-398 induces NF-kB and down-regulates Ikβ-α protein expression in LNCaP C4-2b cells[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
RAW264.7 cells M4LJZWZ2dmO2aX;uJIF{e2G7 M2\1dmV3[Wy3YYTl[EBnd3JiaX7obYJqfGmxbjDv[kBEV1hvMjDjZZRidHm8ZXSgVGdGNTJicILv[JVkfGmxbjDmdo9uKEySUzDpcoR2[2WmIGLBW{AzPjRwNzDj[YxteyxiSVO1NF0xNjVizszN NHHWWVUyPDl6ME[1Oy=>
MDA-MB-231 cell MlXvSpVv[3Srb36gZZN{[Xl? M4j6d3Jm\HWldHnvckBqdiCSR1WyJIxmfmWuczDpckBOTEFvTVKtNlMyKGOnbHy= MXmxOlQ5ODJ5Nx?=
SKBr3 cells NVrQboNFTnWwY4Tpc44h[XO|YYm= M1zVTFI1KGh? M3v4eXJm\HWldHnvckBqdiCFWWCxPUBuWk6DIHX4dJJme3Orb36gbY4hW0uEckOgZ4VtdHNiYX\0[ZIhOjSqIHX4dI9{fXKnIITvJFI2fU1icnXsZZRqfmVidH:gZ49vfHKxbB?= NHPSVWUyPjR6MEK3Oy=>
SK-BR-3 cells M2XNOGN6fG:2b4jpZ4l1gSCjc4PhfS=> M4PC[FI1KGh? NEXmTlNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUUy2EUj2zJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[XlicnXsZZRqfmVidH:gUnM{QThuIFnDOVA:OC55MjFOwG0> MX[xO|A6PTJ{MR?=
SKBR3 cells NX\yc4N1TnWwY4Tpc44h[XO|YYm= MYPJcohq[mm2aX;uJI9nKGG{b33heIF{\SCrbjDoeY1idiCVS1LSN{Bk\WyuczDifUB1emm2aXH0[YQhf2G2ZYKgdoVt\WG|ZTDhd5NigSxiSVO1NF0xNjZ6IN88US=> M{PQZlE5OjdzNUG5
RAW264.7 cells M4KxXWZ2dmO2aX;uJIF{e2G7 MlPzRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIGDHSVIheHKxZIXjeIlwdiCkeTDFTWEtKEmFNUC9OE45KM7:TR?= NETBZ|QzODByNEW3Ni=>
PMA-Ion-stimulated human PBL MnXKSpVv[3Srb36gZZN{[Xl? M3rsTFUh|ryP MmDBNlAhcA>? MoLtTY5pcWKrdHnvckBw\iCFT2iyJIV5eHKnc4Ppc44hcW5iUF3BMWlwdi2|dHnteYxifGWmIHj1cYFvKFCETDDheEA2KHWPIHHmeIVzKDJyIHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= M4q3OFI{ODR5MkOx
HUVEC MVzGeY5kfGmxbjDhd5NigQ>? M{\ITVE5KGh? M1vKPGFvfGmjbnfpc4dmdmmlIHHjeIl3cXS7IHHnZYlve3RiVlXHSmEue3SrbYXsZZRm\CClYYDpcIxienliZHnm[oVz\W62aXH0bY9vKGmwIFjVWmVEKGGodHXyJFE5KGi{czDifUBu[XS{aXflcEBie3OjeR?= NHrhUVczOzFzMES3OS=>
HUVEC NILZcY5HfW6ldHnvckBie3OjeR?= MmTEOFghcA>? MkO5RY51cWGwZ3nv[4VvcWNiYXP0bZZqfHliYXfhbY5{fCCYRVfGRU1{fGmvdXzheIVlKGOnbHygdJJwdGmoZYLheIlwdiCrbjDIWXZGSyCjZoTldkA1QCCqcoOgZpkhSnKmVTDpcoNwenCxcnH0bY9vKGG|c3H5 MlWyNlMyOTB2N{W=
RAW264.7 cells Mni0SpVv[3Srb36gZZN{[Xl? MUGyOEBp NGfXPJlCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViUlHXNlY1NjdiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBNWFNvaX7keYNm\CCSR1WyJJBzd2S3Y4Tpc44h[WSvaX7pd5RmemWmIEGgbJIheHKrb4KgeI8hVFCVLXPoZYxt\W6pZTDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[nliRVnBMEBKSzVyPUCuNFA4ODFizszN MYCyOFM3ODV4MR?=
HaCaT cells M3zURWZ2dmO2aX;uJIF{e2G7 MlzHNlQhcA>? MYrJcohq[mm2aX;uJI9nKFCJRUKgdJJw\HWldHnvckBqdiCqdX3hckBJ[UOjVDDj[YxteyCjZoTldkAzPCCqcoOgZpkhWkmDLDDJR|UxRTBwMEGg{txO MYSxOVM5PzZ2Mh?=
RAW264.7 cells NFL5eHBHfW6ldHnvckBie3OjeR?= M3LFdWlvcGmkaYTpc44hd2ZiUFfFNkBxem:mdXP0bY9vKGmwIFzQV{1qdmS3Y3XkJI1wfXOnIGLBW|I3PC55IHPlcIx{NCCLQ{WwQVAvODB5IN88US=> MX6yOVQ2OzhyMB?=
RAW264.7 cells MkLLSpVv[3Srb36gZZN{[Xl? M1XQelE4NTJyIHi= M{TTUmlvcGmkaYTpc44hd2ZiSV\OMYdidW2jL1zQV{1qdmS3Y3XkJHBITTJicILv[JVkfGmxbjDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjZoTldkAyPyC2bzCyNEBpenNiYomgSWlCKG2ndHjv[EwhUUN3ME2wMlEh|ryP MWCyOVAzPzl|Mx?=
SK-BR-3 cells MYLGeY5kfGmxbjDhd5NigQ>? M2PJPGlvcGmkaYTpc44hd2ZiQ2nQOFUxKGG{b33heIF{\SCjY4Tpeol1gSCrbjDTT{1DWi1|IHPlcIx{NCCLQ{WwQVAvPjhizszN MoXqNVY1QDB{N{e=
RAW264.7 cells NYnMV|BxTnWwY4Tpc44h[XO|YYm= M2LpcGlvcGmkaYTpc44hd2ZiQ1;YNk1u\WSrYYTl[EBRT0V{IIDyc4R2[3Srb36gbY4hVFCVLYP0bY12dGG2ZXSgcY92e2ViUlHXNlY1NjdiY3XscJMh[nliZX76fY1mKGmvbYXuc4F{e2G7LDDJR|UxRTBwMEWg{txO NVjMPZIxOTl{M{O2OFY>
K562 cells NEnKV|ZHfW6ldHnvckBie3OjeR?= MU[0JIRigXN? M1HPbmlvcGmkaYTpc44hd2ZiQ1;YNkBqdiCqdX3hckBMPTZ{IHPlcIx{KGG|c3Xzd4VlKGG|IHLsc4Ns[WSnIH;mJGFOVDFvRWTPJJBzd3SnaX6t[IVx\W6mZX70JIVzgXSqcn;p[EBlcW[oZYLlcpRq[XSrb36gZYZ1\XJiNDDkZZl{KGK7IHLlcppq\GmwZTDzeIFqdmmwZzDt[ZRpd2R? NUPpN|JtOTlzN{KxOFY>
RAW264.7 cells NXHTW401TnWwY4Tpc44h[XO|YYm= NYPTUHh[UW6qaXLpeIlwdiCxZjDDU3gzKGmwIH3veZNmKFKDV{K2OE44KGOnbHzzJIJ6KGWweont[UBqdW23bn;hd5NigSxiSVO1NF0xNjhzIN88US=> NHPNT4EzODB3NkW0PS=>

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot

PubMed: 31410206     

A549 and H1299 cells treated with 10058-F4 and AZD5153 for 48 hours as indicated concentration and c-myc and GAPDH were detected by western blotting. Images are representatives of three independent experiments. 

p-CHK1 / CHK1 ; 

PubMed: 29636547     

Western Blot analysis of OVCAR3 cells treated with increasing concentrations of AZD5153 (2 hour treatment).

LEF1 / active-β-catenin / CXCR4 / Cleaved Caspase 3 ; 

PubMed: 30304769     

Western blot analysis of MYC, active β−catenin, CXCR4, CD45/PTPRC, MYB, and LEF1 in UP-ALL13 cells treated in vitro with vehicle or AZD5153 (50–500 nM) for 18 h. Cleaved caspase 3 is used as a marker of apoptosis. β-actin is shown as loading control.

31410206 29636547 30304769
Growth inhibition assay
Cell viability; 

PubMed: 30304769     

Effect of BRD4 inhibition on the viability of UP-ALL13 and the T-ALL cell lines CUTLL1, DND41, MOLT-16, and SKW3/KE-37. Viability was evaluated after 24 h of incubation with increasing doses of AZD5153. 

In vivo NS398 could inhibit Cox-2 expression induced by acoustic injury and could attenuate noise-induced hearing threshold shifts and cochlear hair cell loss. The inhibition of Cox-2 by NS398 could attenuate Noise-induced hearing loss(NIHL)and related hair cell damage.[3].


Cell Research:[2]
- Collapse
  • Cell lines: human prostate carcinoma cell line LNCaP and the LNCaP subline C4-2b
  • Concentrations: 10 μM
  • Incubation Time: 24, 48, 72 h
  • Method: 1×106 cells are plated in six well cluster plates with 2 ml of medium for 24 h. At time point 0, medium was removed, cells were carefully washed with phosphate buffered saline (PBS) and serum free(SF), phenol-red free medium containing 0.5 μg/ml BSA was added. Incubations are continued for an additional 72 h with or without increasing NS-398 concentrations. NS-398 stocks (5 mM) are dissolved in 0.1% dimethylsulfoxide (DMSO). Cells and culture medium are harvested at 24 h time intervals. Dead cells are removed by gentle washing with PBS and cell number determined by direct counting using trypan blue dye exclusion to identify viable cells. DMSO (0.1%) is added to control cultures.
    (Only for Reference)
Animal Research:[3]
- Collapse
  • Animal Models: CD1 mice
  • Formulation: Normal saline
  • Dosages: 20 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 62 mg/mL (197.22 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 314.36


CAS No. 123653-11-2
Storage powder
in solvent
Synonyms N-(2-cyclohexyloxy-4-nitrophenyl)methane sulfonamide

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID