(-)-Epicatechin gallate

For research use only.

Catalog No.S3925 Synonyms: (−)-ECG, (−)-Epicatechin 3-gallate, (−)-Epicatechin-3-O-gallate

3 publications

(-)-Epicatechin gallate Chemical Structure

CAS No. 1257-08-5

(-)-Epicatechin gallate (ECG) is an inhibitor of cyclooxygenase-1 (COX-1) with IC50 of 7.5 μM.

Selleck's (-)-Epicatechin gallate has been cited by 3 publications

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Biological Activity

Description (-)-Epicatechin gallate (ECG) is an inhibitor of cyclooxygenase-1 (COX-1) with IC50 of 7.5 μM.
In vitro

Treatment of HuH7 cells with (–)-Epigallocatechin gallate (EGCG) causes a time- and dose-dependent decrease in the expression of VEGFR-2 and p-VEGFR-2 proteins. EGCG effectively suppresses the growth of HuH7 human HCC cells both in vitro and in vivo[1]. EGCG potently modulates cardiac excitation contraction coupling by acting on RyR and possibly on L-type Ca2+-channel and NaCa exchanger[2].

In vivo Drinking of EGCG significantly inhibits the growth of HuH7 xenografts in nude mice and this is associated with inhibition of the activation of VEGFR-2 and its related downstream signaling molecules, including ERK and Akt. EGCG drinking also decreases the expression of Bcl-xL protein and VEGF mRNA in the xenografts[1].

Protocol

Cell Research:[1]
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  • Cell lines: HuH7 or Hc cells
  • Concentrations: 0-100 μg/mL
  • Incubation Time: 48 h
  • Method: Three thousand HuH7 or Hc cells are seeded into 96-well plates. Twenty-four hours later, the cells are treated with the indicated concentrations of EGCG (0–100 μg/mL) for 48 h in DF10 medium, and cell viability is examined. All assays are carried out in triplicate.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: BALB/c nude mice (5 weeks of age)
  • Dosages: 0.01 or 0.1%
  • Administration: oral administration
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 88 mg/mL (198.92 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 442.37
Formula

C22H18O10

CAS No. 1257-08-5
Storage powder
in solvent
Synonyms (−)-ECG, (−)-Epicatechin 3-gallate, (−)-Epicatechin-3-O-gallate
Smiles C1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=C(C=C3)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID