(-)-Epicatechin gallate

Catalog No.S3925 Synonyms: (−)-ECG, (−)-Epicatechin 3-gallate, (−)-Epicatechin-3-O-gallate

For research use only.

(-)-Epicatechin gallate (ECG) is an inhibitor of cyclooxygenase-1 (COX-1) with IC50 of 7.5 μM.

(-)-Epicatechin gallate Chemical Structure

CAS No. 1257-08-5

Selleck's (-)-Epicatechin gallate has been cited by 3 Publications

Purity & Quality Control

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Biological Activity

Description (-)-Epicatechin gallate (ECG) is an inhibitor of cyclooxygenase-1 (COX-1) with IC50 of 7.5 μM.
In vitro

Treatment of HuH7 cells with (–)-Epigallocatechin gallate (EGCG) causes a time- and dose-dependent decrease in the expression of VEGFR-2 and p-VEGFR-2 proteins. EGCG effectively suppresses the growth of HuH7 human HCC cells both in vitro and in vivo[1]. EGCG potently modulates cardiac excitation contraction coupling by acting on RyR and possibly on L-type Ca2+-channel and NaCa exchanger[2].

In vivo Drinking of EGCG significantly inhibits the growth of HuH7 xenografts in nude mice and this is associated with inhibition of the activation of VEGFR-2 and its related downstream signaling molecules, including ERK and Akt. EGCG drinking also decreases the expression of Bcl-xL protein and VEGF mRNA in the xenografts[1].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: HuH7 or Hc cells
  • Concentrations: 0-100 μg/mL
  • Incubation Time: 48 h
  • Method: Three thousand HuH7 or Hc cells are seeded into 96-well plates. Twenty-four hours later, the cells are treated with the indicated concentrations of EGCG (0–100 μg/mL) for 48 h in DF10 medium, and cell viability is examined. All assays are carried out in triplicate.
  • (Only for Reference)
Animal Research:[1]
  • Animal Models: BALB/c nude mice (5 weeks of age)
  • Dosages: 0.01 or 0.1%
  • Administration: oral administration
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 88 mg/mL
(198.92 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 442.37
Formula

C22H18O10

CAS No. 1257-08-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=C(C=C3)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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