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Catalog No.S3023

5 publications

Bufexamac Chemical Structure

CAS No. 2438-72-4

Bufexamac is a COX inhibitor for IFN-α release with EC50 of 8.9 μM.

Selleck's Bufexamac has been cited by 5 publications

2 Customer Reviews

  • (a) Treatment of HeLa cells with 1 mM bufexamac results in the induction of HIF1-α, as determined by immunoblotting. The known hypoxia mimetics CPX and DFX were used as positive controls; vinculin served as loading control. (b) Verification of bufexamac-specific upregulation of HIF1-α. HIF1-α protein expression was analyzed 4 h after treatment of cells with the indicated compounds. Ac, acetylated. (c) Time-course analysis of HIF1-α induction by bufexamac. The induction of HIF1-α protein was analyzed by immunoblotting. The graph shows rapid stabilization of HIF1-α upon bufexamac treatment with half-maximal intensity I1/2 of about 75 min. Error bars represent s.d. of three independent experiments. (d) HIF1-α is stabilized only at higher concentrations of bufexamac and accumulates in the nucleus. Induction of endogenous HIF1-α and its nuclear accumulation was analyzed using immunofluorescence microscopy; scale bar, 10 μm.

    Nat Biotechnol, 2015, 33(4):415-23.. Bufexamac purchased from Selleck.

    G. co-IP assay examining the interactions of HDAC10 in 293T cells. Equal amounts of IP products were loaded and subjected to anti-AKT or anti-HDAC10 blotting. The membrane used for anti-HDAC10 blotting (middle panel) was reblotted with anti-p-AKT antibodies (bottom panel). The assay was repeated for three times.

    Oncotarget, 2016, 7(37):59388-59401. Bufexamac purchased from Selleck.

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Biological Activity

Description Bufexamac is a COX inhibitor for IFN-α release with EC50 of 8.9 μM.
Features A specific inhibitor of class IIB histone deacetylases.
IFN-α [1]
8.9 μM
In vitro

Bufexamac is a specific inhibitor of class IIB histone deacetylases (HDAC6 and HDAC10). Treatment of peripheral blood mononuclear cells with bufexamac inhibits the secretion of IFN-α. [1] Bufexamac is a frequent and relevant contact sensitizer. Bufexamac is a non-steroidal anti-inflammatory drug. [2]


Solubility (25°C)

In vitro DMSO 45 mg/mL (201.54 mM)
Water Insoluble
Ethanol '3 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 223.27


CAS No. 2438-72-4
Storage powder
in solvent
Synonyms N/A

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COX Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID