For research use only.

Catalog No.S1180 Synonyms: NVP-XAV939

200 publications

XAV-939 Chemical Structure

CAS No. 284028-89-3

XAV-939 (NVP-XAV939) selectively inhibits Wnt/β-catenin-mediated transcription through tankyrase1/2 inhibition with IC50 of 11 nM/4 nM in cell-free assays, regulates axin levels and does not affect CRE, NF-κB or TGF-β.

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Selleck's XAV-939 has been cited by 200 publications

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Biological Activity

Description XAV-939 (NVP-XAV939) selectively inhibits Wnt/β-catenin-mediated transcription through tankyrase1/2 inhibition with IC50 of 11 nM/4 nM in cell-free assays, regulates axin levels and does not affect CRE, NF-κB or TGF-β.
TNKS2 [1]
(Cell-free assay)
TNKS1 [1]
(Cell-free assay)
4 nM 11 nM
In vitro

XAV-939 specifically inhibits tankyrase PARP activity. XAV-939 dramatically decreases DNA-PKcs protein levels, confirming the critical role of tankyrase poly-ADP-ribosylation activity in maintaining stability of the DNA-PKcs protein. The greatest reduction of DNA-PKcs protein levels (< 25% relative expression compared to DMSO treated controls) occurs at 12 hours with 1.0 μM XAV-939 exposure. Treatment of human lymphoblasts with 1.0 μM XAV-939 results in marked elevation of tankyrase 1 levels. [1] XAV-939 is axin stabilizing agent. XAV-939 stimulates beta-catenin degradation by stabilizing axin, the concentration-limiting component of the destruction complex. XAV-939 stabilizes axin by blocking the poly-ADP-ribosylating enzymes tankyrase 1 and tankyrase 2. Both tankyrase isoforms interact with a highly conserved domain of axin and stimulate its degradation through the ubiquitin-proteasome pathway. XAV-939 deregulates the Wnt/b-catenin pathway which has been implicated in many cancers. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Sf-21 NYPINIdvU2mwYYPlJGF{e2G7 M3;BV|E5Njd3IN88US=> M2j0b|YxKG2rbh?= MkXSSG1UVw>? M4HWRmlvcGmkaYTpc44hd2ZiTj30[ZJucW6jbDDHV3QufGGpZ3XkJHRPU1N{IHX4dJJme3OnZDD3bZRpKEmFNUCgc4YhOC5yMEWzJO69VQ>? MYqyN|g4QTR|MR?=
HEK293T MWLGeY5kfGmxbjDBd5NigQ>? MlTGSG1UVw>? MVfJcohq[mm2aX;uJI9nKFewdDDzbYdv[WyrbnegZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDmc5J{c2:uaX6tbY5lfWOnZDDjRW1RKHKnc4DvcpNmKGWuZX3lcpQh[WO2aY\heIlwdiC5aYToJGlEPTBib3[gNE4xPzhizszN NYfMcIc2OjN6N{m0N|E>
HEK293T MUPGeY5kfGmxbjDBd5NigQ>? M1zrPVExKM7:TR?= MYnEUXNQ NEK0dG1KdmirYnn0bY9vKG:oIHLleIEu[2G|ZXnuMYRmeGWwZHXueEBk[W6xbnnjZYwhX262MzDwZZRpf2G7IIfpeIghUUN3MDDv[kAxNjB3MTFOwG0> M4f6VFIzOTlzNUW3
HEK293T M{OzcGZ2dmO2aX;uJGF{e2G7 M{m5cFI1KGh? MlXZSG1UVw>? NFn2OpFKdmirYnn0bY9vKG:oIH3veZNmKFewdEPBJJNq\26jbHnu[{B4cXSqIFnDOVAhd2ZiMD6wO|gh|ryP MXOyNlI3ODJyMx?=
SW480 MkTrSpVv[3Srb36gRZN{[Xl? MXuxNEDPxE1? NHfGcnczPCCq NFnsU5VFVVOR MlzrV5Ri[mmuaYrheIlwdiCxZjDBfIlvOiC5aYToJGVEPTBib3[gNE4{PzFizszN MYKyNlI3ODJyMx?=
HEK293T MYfGeY5kfGmxbjDBd5NigQ>? MX21NEDPxE1? MWXEUXNQ MW\IZZMhdm9iRX\m[YN1KG:wIH\vdpNsd2yrbj3pcoR2[2WmIHPBUXAhe2mpbnHsbY5oKGmwIHj1cYFvKEiHS{K5N3Qh[2WubIOgZ49mgHC{ZYPzbY5oKEOURR?= MoX0NlIzPjB{MEO=
IEC-6 NIP0SJlHfW6ldHnvckBCe3OjeR?= NELaZXI3KGh? M{\XdmFvfGGpb37pd5Qh[WO2aY\peJkh[XRiQnX0ZU1k[XSnbnnuM3RETiCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFewdD2zZU1qdmS3Y3XkJIF5cW5{IHX4dJJme3Orb36ge4l1cCCLQ{WwJI9nKDBwNkSg{txO MnT3NlQxPjB2OEm=
IEC-6 MoXCSpVv[3Srb36gRZN{[Xl? MWS2JIg> MX\BcpRi\2:waYP0JIFkfGm4aYT5JIF1KEKndHGtZ4F1\W6rbj;UR2Yh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBYdnRvM3GtbY5lfWOnZDDs[5I2KGW6cILld5Nqd25id3n0bEBKSzVyIH;mJFIvQSEQvF2= M4n5NFI1ODZyNEi5
DLD1 M1XhTGZ2dmO2aX;uJGF{e2G7 MVGyNEDPxE1? NET6XY4zPCCq NV\JS246TE2VTx?= MVrJcohq[mm2aX;uJI9nKHSjbnv5doF{\SCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFSFRj3k[ZBmdmSnboSgeJJidnOlcnnweIlwdmGuIHHjeIl3cXS7 MWOyOFUzPzd7Mh?=
DLD1 NXz6cldnS3m2b4TvfIlkKEG|c3H5 NVLnfZRPOjBizszN NVnMVWZOOTBiZB?= M1T1TWROW09? MkfjR5l1d3SxeHnjbZR6KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:w NFrKepgzPDV{N{e5Ni=>
VERO M{DrO2Z2dmO2aX;uJGF{e2G7 MUmyOUDPxE1? NXr6foo3TE2VTx?= MmfQSIl{fHW{YnXzJHBCWiCkZXz0JJN6dnSqZYPpd{wh[W[oZXP0bY5oKHSqZTDhZ5RqdiCleYTvd4tmdGW2b36sJINmdGxic3jhdIUh[W6mIHPlcIwh[WSqZYPpc44> NVnFXo54OjV|M{K4OFU>
HeLa M4DZTWZ2dmO2aX;uJGF{e2G7 MYGxNEDPxE1? M1rmblQ5KGh? NX30OJN[WmWmdXP0bY9vKG:oIHP5eI9xdGG|bXnjJIRqe3S{aXL1eIlwdiCjbnSgcpVkdGWjcjD0doFve2yxY3H0bY9vKG:oIN8yMYNifGWwaX6= MmfENlUxPjF2OUm=
SiHa MkjLSpVv[3Srb36gRZN{[Xl? MkXlNVAh|ryP MoDQOFghcA>? NXrpVoxoWmWmdXP0bY9vKG:oIHP5eI9xdGG|bXnjJIRqe3S{aXL1eIlwdiCjbnSgcpVkdGWjcjD0doFve2yxY3H0bY9vKG:oIN8yMYNifGWwaX6= NFq0NYwzPTB4MUS5PS=>

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
β-catenin / E-cadherin / N-cadherin; 

PubMed: 31060551     

Western blot of the β-catenin, E-cadherin and N-cadherin proteins after treatment with the β-catenin inhibitor XAV-939 (100 μM) for 48 h in the indicated cells. β-Tubulin was used as a loading control. 

β-catenin / c-Myc / cyclin D1 / E-cadherin / N-cadherin / Vimentin ; 

PubMed: 28177909     

In the fibulin-4 shRNA-infected cells, Wnt signaling pathway inhibitor XAV-939 could significantly inhibit the Wnt/β-catenin pathway and EMT, both of which were activated by fibulin-4 knockdown.

31060551 28177909
β-catenin / E-cadherin ; 

PubMed: 31060551     

Immunofluorescence of subcellular β-catenin and E-cadherin localization after treatment with the β-catenin inhibitor XAV-939 (100 μM) for 48 h in the indicated cells.

Actin / PAR; 

PubMed: 25332845     

(A)-(C) Actin (red), (D)-(F) PAR (green), (G)-(I) merge. (A, D, G) control, (B, E, H) 3 mM EGTA, (C, F, I) 25 µM XAV 939. Bar: 25 µm.

Hes1 / β-catenin / p-STAT3; 

PubMed: 25061499     

Immunofluorescent staining of Hes1 in HeLa and SiHa cells without (N) and with (L) 8 µM L685,458 treatment for 48h. Immunofluorescent staining of β-catenin in HeLa and SiHa cells without (N) and with (XAV) 10 μM XAV-939 treatment for 48h. Immunofluorescent staining of p-STAT3 in HeLa and SiHa cells without (N) and with (AG) 80 μM AG490 treatment for 48h.

31060551 25332845 25061499


Cell Research:[1]
- Collapse
  • Cell lines: WTK1 lymphoblasts
  • Concentrations: 1.0 μM
  • Incubation Time: 8 hours
  • Method: XAV-939 is solubilized in DMSO at 55 °C to make a 10 mM stock solution which may be diluted later to a working concentration of 100 μM. WTK1 lymphoblasts treated with either DMSO or 1.0 μM XAV-939 for 8 hours are loaded into independent wells of a 4-20% gradient SDS-PAGE every 2 hours over the course of 6 hours. At each time point, DMSO and XAV-939 samples are loaded into wells immediately adjacent to the prior time point. The corresponding load times at 0, 2 and 4 hours results in total run times of 2, 4 and 6 hours respectively. The gel is analyzed via western blot for DNA-PKcs following completion of the final run time and is quantified after normalization to actin loading controls.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 12 mg/mL (38.42 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+corn oil
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 312.31


CAS No. 284028-89-3
Storage powder
in solvent
Synonyms NVP-XAV939
Smiles C1CSCC2=C1N=C(NC2=O)C3=CC=C(C=C3)C(F)(F)F

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Frequently Asked Questions

  • Question 1:

    I want to inject XAV 939 (Cat # S1180) into mice through I.P. and just wonder what kind of solvent/solution I can use for this.

  • Answer:

    S1180 XAV-939 can be dissolved in 4% DMSO+corn oil at 1 mg/ml as a clear solution. When preparing the solution, please dissolve the compound in DMSO clearly first. You can sonicate and warm it in water bath at about 45 degree to help dissolving. Then dilute with corn oil.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID