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PJ34 HCl

For research use only.

Catalog No.S7300

26 publications

PJ34 HCl  Chemical Structure

CAS No. 344458-15-7

PJ34 HCl is the hydrochloride salt of PJ34, which is a PARP inhibitor with EC50 of 20 nM and is equally potent to PARP1/2.

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Selleck's PJ34 HCl has been cited by 26 publications

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Biological Activity

Description PJ34 HCl is the hydrochloride salt of PJ34, which is a PARP inhibitor with EC50 of 20 nM and is equally potent to PARP1/2.
Features Water-soluble PARP1/2 inhibitor with >10,000-fold potentcy vs. 3-aminobenzamide (prototypical PARP inhibitor). Potential uses in cardiovascular diseases (stroke, cerebral ischemia, & myocardial ischemia).
PARP [1]
(Cell-free assay)
20 nM(EC50)
In vitro

PJ34 is a potent, phenanthridinone PARS inhibitor, which is approximately 10,000 times more potent than the prototypical PARS inhibitor 3-aminobenzamide. PJ34 inhibited peroxynitrite-induced cell necrosis with EC50 of 20 nM. PJ34 provides cardioprotection by decreasing myocardial infarct size and enhancing postischemic regional and global functional recovery. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa cells NX;ubI1HTnWwY4Tpc44h[XO|YYm= MXvJcohq[mm2aX;uJI9nKFCDUmCxJIlvKGi3bXHuJGhmVGFiY3XscJMh[nliZnz1bYQhe2OrboTpcIxifGmxbjDjc5VvfGmwZzD1d4lv\yCdYXTlcplt[XSnZD2zNnBeVkGGIHHzJJN2[nO2cnH0[UwhUUN3ME2wMlAzKM7:TR?= NHHTWnYyQDdzM{[2OS=>
Escherichia coli Rosetta2 (DE3) cells MUDGeY5kfGmxbjDhd5NigQ>? NWrhZmI3UW6qaXLpeIlwdiCxZjDoeY1idiCQLYTldo1qdmGuIE\4bIl{NXSjZ3fl[EBCWlSGNjCoPFc{KHSxIEGxOlEqKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIGLvd4V1fGF{IDjESVMqKGOnbHzzJJV{cW6pIF7BSEsh[XNic4Xid5Rz[XSnIHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3ME2wMlIyQDd6IN88US=> MXyyOFkxODd5MB?=
Escherichia coli BL21 Star (DE3) cells Mn;DSpVv[3Srb36gZZN{[Xl? MkXqTY5pcWKrdHnvckBw\iB4eHjpd{11[WepZXSgRXJVTDViKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyKFO2YYKgLGRGOyliY3XscJMtKEmFNUC9NE42PyEQvF2= NGPOeI8zPDlyMEe3NC=>
MEF cells NUHLd4lUWHKxbHnm[ZJifGmxbjDhd5NigQ>? NH\xU4M4OiCq MVrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IF3FSkBk\WyuczDoZZJjd3KrbnegRpJk[TFiZHXs[ZRqd25ibYX0ZY51KGG2IHX4c44hOTFiYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yOy5{IN88US=> NUC4NHZbOjJ|NkW1OlM>
MEF cells NIC3N2NRem:uaX\ldoF1cW:wIHHzd4F6 NUTqcXdOPzJiaB?= NXXub5J6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEB4cWymIIT5dIUhVUWIIHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVkvQCEQvF2= NHPi[m4zOjN4NUW2Ny=>

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
p53 / pS15-p53 / pChk1 / pChk2; 

PubMed: 21840268     

Western blots on PJ34 treated U2OS cells for the indicated proteins. pChk1 = phospho(Serine345)-Chk1, pChk2 = phospho(Threonine68)-Chk2, pS15p53 = phospho(serine15)-p53. Tubulin is shown as a loading control.

p-p53 / p53 ; 

PubMed: 28095779     

Higher amounts of p53 protein and phosphorylated p53 in NSPCs after PARP inhibition were revealed by immunocytochemistry. Representative images from 4 separate experiments are shown.

In vivo PJ34 suppresses the development of clinical signs of EAE in MBP-immunized PLSJL mice. PJ34 exerted therapeutic effects at the onset of EAE that are associated with reduced CNS inflammation and the maintenance of neurovascular integrity. PJ34 partially inhibits the expression of TNF-α and ICAM-1 in the Spinal Cord Tissues of MBP-Immunized Mice.[2] PJ34 provides significant, dose-dependent, anti-inflammatory effects in a variety of local inflammation models. PJ34 dose-dependently suppresses neutrophil infiltration and nitric oxide (but not KC and IL-1β) production in peritonitis. In a model of systemic endotoxemia, PJ34 pretreatment significantly reduces plasma levels of TNF-α, IL-1β and nitrite/nitrate (breakdown products of nitric oxide) production. PJ34 treatment (oral gavage) induces a significant suppression of the inflammatory response in dextran sulfate colitis, multiple low dose streptozotocin diabetes and cyclophosphamide-accelerated autoimmune diabetes in the non-obese diabetic mice, and reduces the degree of mononuclear cell infiltration into the iris in an endotoxin-induced uveitis model. [3]


Animal Research:[2]
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  • Animal Models: Female PLSJL mice
  • Dosages: 10 mg/kg b.wt. twice daily
  • Administration: orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 66 mg/mL (198.91 mM)
Water 66 mg/mL (198.91 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
15 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 331.8


CAS No. 344458-15-7
Storage powder
in solvent
Synonyms N/A
Smiles CN(C)CC(=O)NC1=CC2=C(C=C1)NC(=O)C3=CC=CC=C32.Cl

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% DMSO % % Tween 80 % ddH2O

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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PARP Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID