PJ34 HCl

Catalog No.S7300

For research use only.

PJ34 HCl is the hydrochloride salt of PJ34, which is a PARP inhibitor with EC50 of 20 nM and is equally potent to PARP1/2.

PJ34 HCl  Chemical Structure

CAS No. 344458-15-7

Selleck's PJ34 HCl has been cited by 36 publications

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Biological Activity

Description PJ34 HCl is the hydrochloride salt of PJ34, which is a PARP inhibitor with EC50 of 20 nM and is equally potent to PARP1/2.
Features Water-soluble PARP1/2 inhibitor with >10,000-fold potentcy vs. 3-aminobenzamide (prototypical PARP inhibitor). Potential uses in cardiovascular diseases (stroke, cerebral ischemia, & myocardial ischemia).
Targets
PARP [1]
(Cell-free assay)
20 nM(EC50)
In vitro

PJ34 is a potent, phenanthridinone PARS inhibitor, which is approximately 10,000 times more potent than the prototypical PARS inhibitor 3-aminobenzamide. PJ34 inhibited peroxynitrite-induced cell necrosis with EC50 of 20 nM. PJ34 provides cardioprotection by decreasing myocardial infarct size and enhancing postischemic regional and global functional recovery. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Escherichia coli BL21(DE3) cells NELL[plHfW6ldHnvckBie3OjeR?= MVWxNEB2VQ>? MV7Jcohq[mm2aX;uJI9nKGi3bXHuJINwem:wYY\pdpV{KE:FNEOgcpVkdGWxY3Hwd4llKHC{b4TlbY4h\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQlyyNUhFTTNrIHHzd4V{e2WmIHHzJJJm\HWldHnvckBw\iCUTlGtZolv\GmwZzDjZZBi[2m2eTDifUBu\WG|dYLpcochemW|b37hcoNmKHWwaYSgZZQhOTBidV2gZpkhe3W{ZnHj[UBxdGG|bX;uJJJme2:wYX7j[UBidmGueYPpd{Bz\WyjdHn2[UB1dyClb370do9t MlryQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3NkS2NFgoRjJ2NU[0OlA5RC:jPh?=
HeLa MkfsSpVv[3Srb36gZZN{[Xl? M3zDSWlvcGmkaYTpc44hd2ZiUFHSVFEhcW5iaIXtZY4hUGWOYTDj[YxteyCkeTDmcJVq\CC|Y3nueIltdGG2aX;uJINwfW62aX7nJJV{cW6pIGvh[IVvgWyjdHXkMVMzWF2QQVSgZZMhe3Wkc4TyZZRmNCCLQ{WwQVAvODMQvF2u MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDdzM{[2OUc,OTh5MUO2OlU9N2F-
Escherichia coli Rosetta2 (DE3) MmXnSpVv[3Srb36gZZN{[Xl? MXLJcohq[mm2aX;uJI9nKGi3bXHuJG4ufGW{bXnuZYwhPniqaYOteIFo\2WmIFHSWGQ3KCh6N{OgeI8hOTF4MTmg[ZhxemW|c3XkJIlvKEW|Y3jldolkcGmjIHPvcIkhWm:|ZYT0ZVIhMESHMzmgZ4VtdHNidYPpcochVkGGKzDhd{B{fWK|dILheIUh[nliZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxRTBwMkG4O|jPxE1w MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEe3NEc,OjR7MEC3O|A9N2F-
Escherichia coli Rosetta2 (DE3) NHfhbWRHfW6ldHnvckBie3OjeR?= M2nU[WlvcGmkaYTpc44hd2ZiaIXtZY4hVi22ZYLtbY5idCB4eHjpd{11[WepZXSgRXJVTDZiKEi3N{B1dyBzMU[xLUBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCUb4PleJRiOiBqRFWzLUBk\WyuczD1d4lv\yCQQVSrJIF{KHO3YoP0doF1\SCkeTDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTB;MD6yNVnPxE1w NYq5WIkxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA4PzBpPkK0PVAxPzdyPD;hQi=>
Escherichia coli BL21 Star (DE3) Mn\SSpVv[3Srb36gZZN{[Xl? MYHJcohq[mm2aX;uJI9nKD[6aHnzMZRi\2enZDDBVnRFPSBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEhW3SjcjCoSGU{MSClZXzsd{whUUN3ME2wMlU4|ryPLh?= NHT0Wok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFc4OCd-MkS5NFA4PzB:L3G+
Escherichia coli Rosetta2 (DE3) MonDSpVv[3Srb36gZZN{[Xl? NUDjV2JRUW6qaXLpeIlwdiCxZjDoeY1idiB4eHjpd{11[WepZXSgRXJVTDViKEGwN|AhfG9iMUOxO{kh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliUn;z[ZR1[TJiKFTFN{kh[2WubIOgeZNqdmdiTlHEL{BieyC|dXLzeJJifGViYomg[ox2d3Knc3PlcoNmKGG|c3H5MEBKSzVyPUGuN:69VS5? Mn[4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC3O|AoRjJ2OUCwO|cxRC:jPh?=
Escherichia coli Rosetta2 (DE3) MX7GeY5kfGmxbjDhd5NigQ>? NF6yTWVKdmirYnn0bY9vKG:oIHj1cYFvKD[6aHnzMZRi\2enZDDBVnRFPSBqMUCzNEB1dyBzM{G3LUBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCUb4PleJRiOiBqRFWzLUBk\WyuczD1d4lv\yCQQVSrJIF{KHO3YoP0doF1\SCkeTDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTB;MT6zOFg6Ps7:TT6= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEe3NEc,OjR7MEC3O|A9N2F-
MEF NVnpUVZ6SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M2D3TlczKGi{cx?= M3L4fmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgUWVHKGOnbHzzJIhiemKxcnnu[{BDemOjMTDk[YxmfGmxbjDteZRidnRiYYSg[ZhwdiBzMTDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTF|LkNOwG0v MkjSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|NkW1OlMoRjJ{M{[1OVY{RC:jPh?=
MEF NFTsVJhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MVq3NkBpenN? NX\SWVUxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEB4cWymIIT5dIUhVUWIIHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVkvQM7:TT6= NWKx[oNRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzOlU2PjNpPkKyN|Y2PTZ|PD;hQi=>
Assay
Methods Test Index PMID
Western blot p53 / pS15-p53 / pChk1 / pChk2 21840268
Immunofluorescence p-p53 / p53 28095779
In vivo PJ34 suppresses the development of clinical signs of EAE in MBP-immunized PLSJL mice. PJ34 exerted therapeutic effects at the onset of EAE that are associated with reduced CNS inflammation and the maintenance of neurovascular integrity. PJ34 partially inhibits the expression of TNF-α and ICAM-1 in the Spinal Cord Tissues of MBP-Immunized Mice.[2] PJ34 provides significant, dose-dependent, anti-inflammatory effects in a variety of local inflammation models. PJ34 dose-dependently suppresses neutrophil infiltration and nitric oxide (but not KC and IL-1β) production in peritonitis. In a model of systemic endotoxemia, PJ34 pretreatment significantly reduces plasma levels of TNF-α, IL-1β and nitrite/nitrate (breakdown products of nitric oxide) production. PJ34 treatment (oral gavage) induces a significant suppression of the inflammatory response in dextran sulfate colitis, multiple low dose streptozotocin diabetes and cyclophosphamide-accelerated autoimmune diabetes in the non-obese diabetic mice, and reduces the degree of mononuclear cell infiltration into the iris in an endotoxin-induced uveitis model. [3]

Protocol (from reference)

Animal Research:[2]
  • Animal Models: Female PLSJL mice
  • Dosages: 10 mg/kg b.wt. twice daily
  • Administration: orally

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.

15 mg/mL

Chemical Information

Molecular Weight 331.8
Formula

C17H17N3O2.HCl

CAS No. 344458-15-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CN(C)CC(=O)NC1=CC2=C(C=C1)NC(=O)C3=CC=CC=C32.Cl

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

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