Catalog No.S2618 Synonyms: DM3189

LDN-193189 Chemical Structure

Molecular Weight(MW): 406.48

LDN-193189 is a selective BMP signaling inhibitor, inhibits the transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 of 5 nM and 30 nM in C2C12 cells, respectively, exhibits 200-fold selectivity for BMP versus TGF-β.

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3 Customer Reviews

  • The small molecule BMP inhibitor LDN-193189 can partially block the increased BMP signaling in Jab1flox/flox, Col2a1-Cre mutants in a dose-dependent manner. Primary chondrocytes were cultured in DMEM medium containing 10% FBS and indicated amounts of LDN-193189 for 24 hours before being subjected to immunoblotting.

    J Cell Sci, 2013, 126: 234-43. LDN-193189 purchased from Selleck.

    LDN induces signifiant cell death. OVCA429 cells were treated with vehicle (DMSO) or experimental drugs for 6 hr to capture different stages of cell death. OVCA429 cell viability was assessed in response to drug treatment for 6 hr by staining with AO (green) and EB (orange). For clarity the different fluorescent channels to detect AO, EB, and the over-lapping images are shown for 10 mM LDN treatment.

    Int J Cancer 2014 136(5):E455-69. LDN-193189 purchased from Selleck.

  • The density of muscle fibers and myoseptum are partially rescued in morphants treated with LDN to decrease BMP signaling (D–F) .These were fixed at 24 hpf and labeled with an antibody against Pax7, which strongly labels neural crest derived cells and also labels dermomyotome cells.

    Dev Biol 2014 378:107-121. LDN-193189 purchased from Selleck.

Purity & Quality Control

Choose Selective TGF-beta/Smad Inhibitors

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2. For more details, such as half maximal inhibitory concentrations (IC50s) and working concentrations of each inhibitor, please click on the link of the inhibitor of interest.
3. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation.
4. Orange "√" refers to compounds which do inhibitory effects on the related isoform, but without specific value.

Biological Activity

Description LDN-193189 is a selective BMP signaling inhibitor, inhibits the transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 of 5 nM and 30 nM in C2C12 cells, respectively, exhibits 200-fold selectivity for BMP versus TGF-β.
ALK2 [1]
(C2C12 cells)
ALK3 [1]
(C2C12 cells)
5 nM 30 nM
In vitro

LDN193189 potently inhibits BMP4-mediated Smad1, Smad5 and Smad8 activation with IC50 of 5 nM, and efficiently inhibits transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 of 5 nM and 30 nM, respectively. Furthermore, LDN193189 also shows the inhibitory effect on the transcriptional activity induced by either constitutively active ALK2R206H or ALK2Q207D mutant proteins. [1] A recent study shows that LDN-193189 blocks the production of reactive oxygen species induced by oxidized LDL during atherogenesis in human aortic endothelial cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
C2C12 M1\wSWtqdmG|ZTDBd5NigQ>? M1;BSYlvcGmkaYTzJJRp\SCtaX7hd4Uh[WO2aY\peJkhd2ZiQVzLOEBidmRiQXP0VmlKSSC5aYToJGlEPTEEoI\hcJVmeyCxZjCxNFEh[W6mIEKxNEBvdSxicnXzdIVkfGm4ZXz5 MkHLNlU{Pjh|MkK=
EOC216 NX3zSpRnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPxNE4yNTFyIN88US=> NFH4[XUzNTFyIHS= NUPMS5VCTE2VTx?= NWixdHZLcW6mdXPlJINmdGxiZHXheIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> MViyOVIzPzh7Mx?=
OVAC429 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXuyM|Uh|ryP NFLTd5A1QCCq MnPHSG1UVw>? MmXB[IVkemWjc3XzJJRp\SCyZYLj[Y51[WenIH;mJINmdGy|IHnuJHMheGijcx?= M33iZlI2OjJ5OEmz
SKOV3 NF76VmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXOyM|Uh|ryP NFjKTpE1QCCq MX\EUXNQ MXLk[YNz\WG|ZYOgeIhmKHCncnPlcpRi\2Vib3[gZ4VtdHNiaX6gV{BxcGG| NXHkcIxwOjV{Mke4PVM>
OVCA429 Mo\qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmHXNk82KM7:TR?= MYS0PEBp NIjBXppFVVOR M17LTIRm[3KnYYPld{B1cGVicHXyZ4VvfGGpZTDv[kBk\WyuczDpckBUKHCqYYO= M4CxdlI2OjJ5OEmz
EOC219 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVeyM|Uh|ryP Mki4OFghcA>? NEDhR2VFVVOR M4\5TIRm[3KnYYPld{B1cGVicHXyZ4VvfGGpZTDv[kBk\WyuczDpckBUKHCqYYO= MYCyOVIzPzh7Mx?=
A549 NHXEWppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUewMlUuOTZizszN NXvKe4ZKTE2VTx?= NUThWVQ4cW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NHXnSHczPDd5OECxNS=>
BEAS-2B  M1;BSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVPXe4NlOC53LUG2JO69VQ>? MWXEUXNQ M3rGSIlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz M1nWTVI1Pzd6MEGx
hBMSCs NXzqbXo1TnWwY4Tpc44hSXO|YYm= NVvMT2FLOC5{wrFOwG0> NFPsOm04KGR? NXHLNGJ3[WKxbHnzbIV{KHSqZTDzbYxq[mmwaX6tdJJwdW:2ZXSgRWxRKGGldHn2bZR6yqCyYYL0cJk> NXTKe|UzOjRyN{[xPFc>
PANC-1 NYXZZ49QT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvTV3Q2NTVyMDDuUS=> Mn\1OFghcA>? MmS3bY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NV\LSZdZOjN7Nkm3Nlk>
MIA PaCa-2 NWG0N2g{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3fTTVUuPTByIH7N NHTHS5E1QCCq MYXpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> M{nUcFI{QTZ7N{K5
Bx-PC3 NIjGNGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYC0T49kPS13MECgcm0> M3HZZlQ5KGh? MUnpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NXPQPXZQOjN7Nkm3Nlk>
PC3  MlnISpVv[3Srb36gRZN{[Xl? MnPaOVAxKG6P MV:yJIg> Mnv6doVxemW|c3XzJIFkfGm4YYTpc44hd2ZiU33h[FEwPS96IHHu[EBRNVOvYXSzJIxmfmWucx?= NYPxTGF5OjJ2NUK4PFM>
LNCaP NE[xOItHfW6ldHnvckBCe3OjeR?= M1:zNVDjiJN3MECgcm0> NXSy[ZNwOiCq MoXydoV3\XK|ZYOgdoFx[W27Y3nuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> MXOyNlQ2Ojh6Mx?=
EOC NE\VWnRHfW6ldHnvckBCe3OjeR?= M33EZ|ExNTFyMECgcm0> M1ziOFczKGh? MXry[YR2[2W|IITo[UBxcG:|cHjvdplt[XSnZDDCUXAhWi2VbXHkNU82NzkEoB?= MmTkNlIzPDl2MUW=
HaCaT  NWC4bGdSTnWwY4Tpc44hSXO|YYm= NFO0[XYxNjByMT2xNEDPxE1? NITmbo4zKGh? M4e5U4lvcGmkaYTzJGJOWDJvaX7keYNm\CCyaH;zdIhwenmuYYTpc44hd2ZiU33h[FEwPS96IIfpeIgh[W5iSVO1NOKhd2ZifkCuNFA2yqEQvF2= NYTPS2p2OjF5NEC5OlY>
HaCaT  MULGeY5kfGmxbjDBd5NigQ>? M3PXVVAvODBzLUGwJO69VQ>? MoW3NkBp MofXbY5pcWKrdIOgeIhmKGGkaXzpeJkhd2ZiQVzLN{B1dyCyaH;zdIhwenmuYYTlJHNu[WRzwrD3bZRpKGGwIFnDOVDDqG:oIEGwNEBvVQ>? NEPmV20zOTd2MEm2Oi=>
HaCaT  NEPOS2dHfW6ldHnvckBCe3OjeR?= NIX6NJIxNjByMT2xNEDPxE1? M3jT[|IhcA>? MkC5bY5pcWKrdIOgRWxMPCCjbnSgRWxMPSC5aYToJGlEPTEEoI\hcJVmeyCxZjCwMlPDqM7:TTDhcoQhOC53wrFOwG0hemW|cHXjeIl3\Wy7 MUeyNVc1ODl4Nh?=

... Click to View More Cell Line Experimental Data

In vivo In conditional caALK2-transgenic mice with Ad.Cre on on postnatal day 7 (P7), LDN-193189 (3 mg/kg i.p) leads to mild calcifications surrounding the left tibia and fibula first visible at P13, and prevents radiographic lesions at P15 without causing weight loss or growth retardation, spontaneous fractures, decreased bone density or behavioral abnormalities. [1] LDN193189 dorsalizes zebrafish embryos by inhibiting signaling pathways induced by bone morphogenetic protein (BMP)6 without effect on vascular development. [2] In PCa-118b tumor-bearing mice, LDN-193189 treatment attenuates tumor growth and reduces bone formation in the tumors. [3] In LDL receptor-deficient (LDLR-/-) mice, LDN-193189 potently inhibits development of atheroma. Moreover, LDN-193189 also exhibits the inhibitory effects on associated vascular inflammation, osteogenic activity, and calcification. [4]


Animal Research:[1]
+ Expand
  • Animal Models: Ad.Cre on P7 is injected into conditional caALK2–transgenic and wild-type mice.
  • Formulation: LDN193189 is dissolved in DMSO and then diluted in water.
  • Dosages: ≤3 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro Ethanol 1 mg/mL (2.46 mM) warming
DMSO <1 mg/mL
Water <1 mg/mL
In vivo Saline (suspension) 30mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 406.48


CAS No. 1062368-24-4
Storage powder
in solvent
Synonyms DM3189

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID