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Rosiglitazone (BRL 49653)

Name: Rosiglitazone (BRL 49653)
Brand: Selleck Chemicals
SKU: S2556
Rating: 5 (51 reviews)

Catalog No.S2556

For research use only.

Rosiglitazone (BRL 49653) is a potent antihyperglycemic agent and a potent thiazolidinedione insulin sensitizer with IC50 of 12, 4 and 9 nM for rat, 3T3-L1 and human adipocytes, respectively. Rosiglitazone is a pure ligand of PPAR-gamma, and has no PPAR-alpha-binding action. Rosiglitazone modulates TRP channels and induces autophagy. Rosiglitazone prevents ferroptosis.

Rosiglitazone (BRL 49653) Chemical Structure

CAS No. 122320-73-4

Selleck's Rosiglitazone (BRL 49653) has been cited by 51 publications

Purity & Quality Control

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PPAR Signaling Pathway Map

Biological Activity

Description

Targets

PPAR ^[1]
(Cell-free assay)

TRPC ^[5]

Ferroptosis ^[6]

In vitro

Rosiglitazone markedly increase phosphorylation of threonine 172 within the α subunit of AMPK, with increased AMP:ATP ratio. Rosiglitazone has been reported to decrease cholesterol synthesis in a number of cell lines in a peroxisome proliferator-activated receptor γ-independent manner. Rosiglitazone activates both α1- and α2-containing AMPK complexes, and this leads to a marked increase in the phosphorylation of acetyl-CoA carboxylase. ^[1] Rosiglitazone activates PPAR-gamma2 which acts as a dominant inhibitor of osteoblastogenesis in murine bone marrow in vitro.^[2] Rosiglitazone increases adiponectin secretion from omental cells up to 2.3-fold higher, whereas secretion from subcutaneous adipose cells is unaffected. ^[3] Rosiglitazone changes the morphological features and protein profiles of mitochondria in 3T3-L1 adipocytes. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

HepG2	M2C3TGZ2dmO2aX;uJIF{e2G7			MV7F[oZm[3SrdnWgZ49v[2WwdILheIlwdiCjZ3HpcpN1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSHXwS\|Ih[2WubIOsJGVEPTB;MD6wNkDPxE1?	M3vIW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OECxPFE4Lz5zNUiwNVgyPzxxYU6=
CV-1	M1XYUmZ2dmO2aX;uJIF{e2G7			MXvJckB3cXS{bzD0doFve2O{aYD0bY9v[WxiYXP0bZZifGmxbjDv[kBR\XKxeHnzc41mKHC{b3zp[oVz[XSxcjDhZ5RqfmG2ZXSgdoVk\XC2b4Kg[4FudWFiKGDQRXIqKGW6cILld5Nm\CCrbjDDWk0yKGOnbHzzMEBGSzVyIE2gNE4xPiEQvF2u	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85PTd4OUC3K\|45PTd4OUC3QE9iRg>?
CV-1	MXPGeY5kfGmxbjDhd5NigQ>?			NGPiWVZC[3SrdnH0bY9vKG:oIIDldo95cXOxbXWgdJJwdGmoZYLheI9zKGGldHn2ZZRm\CC{ZXPldJRweiCpYX3tZUBu\WG\|dYLl[EBjgSCrbnT1Z5Rqd25ib3[gOVAmKG:oIH3hfIlufW1iYXzrZYxqdmVicHjvd5Bp[XSjc3WgZYN1cX[rdImsJJRz[W6\|ZnXjeIlwdiCjc4PhfUBqdiCFVj2xJINmdGy\|LDDFR\|UxKD1iMD6wPFkyOyEQvF2u	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86QDN4NkKwK\|46QDN4NkKwQE9iRg>?
3T3-L1	MnXxSpVv[3Srb36gZZN{[Xl?			M2jqZnN1cW23bHH0bY9vKG:oIHHkbZBw\2WwZYPpd{BqdiB\|VEOtUFEh[2WubIOgbZMh\XiycnXzd4VlKGG\|IHPvcoNmdnS{YYTpc44h\XG3aY\hcIVvfCC2bzD0bIUhYyBzLUG0R30hfXC2YXvlJINwfW62czDh[pRmeiC2cnXheI1mdnRid3n0bEAxNjJidXevcWwhfHKxZ3zpeIF7d26nLDDFR{A:KDBwMEmg{txONg>?	M3X6VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyOUW2NlE{Lz5zMEm1OlIyOzxxYU6=
CV-1	Mo\XSpVv[3Srb36gZZN{[Xl?			NXHzOYlmVWG6aX3hcEBz\XCxcoTldkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDldo95cXOxbXWgdJJwdGmoZYLheI9zKGGldHn2ZZRm\CC{ZXPldJRweiCpYX3tZUBI[Wx2IHPobY1memmlIHnuJJRz[W6\|aXXueIx6KHS{YX7z[oVkfGWmIFPWMVEh[2WubIOgZpkh\nWwY4Tpc45idCCjc4PhfU4tKEWFNUCgQUAxNjB2MzFOwG0v	MlmzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5MkC4OVQoRjFzN{KwPFU1RC:jPh?=
COS1	NGf4VGhHfW6ldHnvckBie3OjeR?=			NEnMVGxC\2:waYP0bYMh[WO2aY\peJkhf2G\|IHTleIVzdWmwZXSgbY4hS0:VMTDj[YxteyC2cnHud4Zm[3SnZDD3bZRpKEeDTDC0MXBRSVJiZ3HtcYEhemWlZYD0c5ItKEWFNUCgQUAxNjB{MTFOwG0v	MmTrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ4MUe5NlQoRjF{NkG3PVI1RC:jPh?=
COS1	M2XDRWZ2dmO2aX;uJIF{e2G7			NF7OdpRKdiC4aYTyc{Bi\2:waYP0JIFkfGm4aYT5JJRme3SnZDDmc5IhfHKjboPhZ5RqfmG2aX;uJIlvKGi3bXHuJHBRSVJiZ3HtcYEuT2GuNDDjbIlu\XKrYzDDU3MuOSClZXzsd{whTUN3MDC9JFAvODJizszNMi=>	NFvMVWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkeyPVY3QCd-MUK3Nlk3Pjh:L3G+
COS1	NV;CV3N5TnWwY4Tpc44h[XO\|YYm=			M3fpRmlvKH[rdILvJJRz[W6\|YXP0bZZifGmxbjDv[kBpfW2jbjDw[ZJwgGm\|b33lJJBzd2yrZnXyZZRweiCjY4TpeoF1\WRicnXj[ZB1d3JiZ3HtcYEhdWWjc4Xy[YQhcW5iUGDBVk1ISUx2IHPobY1memmlIFPPV{0yKGOnbHzzMEBGSzVyIE2gNE4xOiEQvF2u	NWThfXpbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK4O\|M2OTdpPkGyPFc{PTF5PD;hQi=>
CV-1	NHvUT\|ZHfW6ldHnvckBie3OjeR?=			MUfJckB3cXS{bzDleoFtfWG2aX;uJIFo[Wmwc4SgVnhTNWGucHjhM3BRSVJvZ3HtcYEhcW5iQ2[tNUBk\WyuczDifUBkd3S{YX7z[oVkfGmxbjDhd5NigSC5YYOg[IV1\XKvaX7l[EwhTUN3MDC9JFAvOzJ3IN88UU4>	MnHrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ7NUSwOlEoRjF{OUW0NFYyRC:jPh?=
3T3-L1	M2jDS2Z2dmO2aX;uJIF{e2G7			NHLZelNG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDmc5Ih\W6qYX7j[Y1mdnRib3[gbY5{fWyrbj3pcoR2[2WmIITybYdtgWOncnnk[UBi[2O3bYXsZZRqd25iaX6gN3Q{NUxzIHPlcIx{NCCHQ{OwJF0hOC5yMjFOwG0v	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDVyNU[0OEc,OTR3MEW2OFQ9N2F-
HepG2	NE\2ZoxHfW6ldHnvckBie3OjeR?=			MUjF[oZm[3SrdnWgZ49v[2WwdILheIlwdiCjZ3HpcpN1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSHXwS\|Ih[2WubIOsJGVEPTBiPTCwMlA{QSEQvF2u	MmS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZzMEexOVAoRjF4MUC3NVUxRC:jPh?=
U2OS	M2jsNmZ2dmO2aX;uJIF{e2G7			MonzSYZn\WO2IH;uJHBRSVKpYX3tZUB1emGwc3HjeIl3[XSrb36gZYN1cX[rdImgbY4hXTKRUzDj[YxteyxiRVO1NEA:KDBwMEOg{txONg>?	NXrvSZBQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[zNFA6PDRpPkG2N\|AxQTR2PD;hQi=>
Huh7	M3;VNWZ2dmO2aX;uJIF{e2G7			NVHMVY5KTnWwY4Tpc45idCCjY4Tpeol1gSCjdDDoeY1idiCSUFHSJIdidW2jIHnuJGh2cDdiY3XscJMh[nlidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gNE4zOiEQvF2u	NYL6c5A6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[zOlY3ODFpPkG2N\|Y3PjBzPD;hQi=>
Huh7	M3izVGZ2dmO2aX;uJIF{e2G7			Mo\WSYZn\WO2IH;uJHBRSVJiZ3HtcYEhfHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JIlvKEi3aEegZ4VtdHNuIFXDOVAhRSByLkKyJO69VS5?	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjR3MUC4O{c,OTZ2NUGwPFc9N2F-
HEK293	NXjFWmlSTnWwY4Tpc44h[XO\|YYm=			NGnEVolC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnu[JVkfGmxbjDv[kBz\WOncITvdkBqdnSncnHjeIlwdiC5aYToJJN1\XKxaXSgdoVk\XC2b4KgZ49i[3SrdnH0c5IuOSCkeTDFXWZRKGKjc3XkJJJmeG:{dHXyJIdmdmViYYPzZZk>	M4fFbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
HEK293	M2L5S2Z2dmO2aX;uJIF{e2G7			M{HOU2Fod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XO\|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJJJm[2WydH;yJIlvfGW{YXP0bY9vKHerdHigdoV1cW6xaXSgXE1z\WOncITvdkBidHCqYTDifUBGYU[SIHLhd4VlKHKncH;yeIVzKGenbnWgZZN{[Xl?	Mn7QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
CV1	MVfGeY5kfGmxbjDhd5NigQ>?			MYHUdoFve2GldHn2ZZRqd25ib3[gVHBCWmejbX3hJIlvKEOYMTDj[YxteyxiRVO1NEA:KDBwMEe2JO69VS5?	NVLUXpdtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[4NlE4PjlpPkG2PFIyPzZ7PD;hQi=>
NIH3T3	Ml;jSpVv[3Srb36gZZN{[Xl?			NGC2N\|NC[3Srdnn0fUBifCCqdX3hckBRWEGUIHfhcY1iKHS{YX7z[oVkfGWmIHnuJG5KUDOWMzDj[YxteyCkeTDseYNq\mW{YYPlJIFkfGm4aYT5JIF{e2G7LDDFR\|UxKD1iMD6zNkDPxE1w	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjh3NEC4OUc,OTZ6NUSwPFU9N2F-
CV1	NGjnT\|dHfW6ldHnvckBie3OjeR?=			MXnUdoFve2GldHn2ZZRqd25ib3[gbJVu[W5iUGDBVodidW2jIHnuJGNXOSClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlMxQCEQvF2u	NILldlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkm3NFM6OSd-MU[5O\|A{QTF:L3G+
HepG2	NXGyUGJkTnWwY4Tpc44h[XO\|YYm=			MoLURYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFTKGejbX3hJIlvKGFiSHXwS\|Ih[2WubIOgZpkhWFCDUj3HRWw1KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNVU5KM7:TT6=	NIXqV\|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkm3N\|M2QCd-MU[5O\|M{PTh:L3G+
HepG2	NEX0emVHfW6ldHnvckBie3OjeR?=			NHPYVVJC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVJiZ3HtcYEhcW5iSHXwS\|Ih[2WubIOgZpkhWFCDUj3HRWw1KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNVU5KM7:TT6=	NX70UXNvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5O\|k{PDFpPkG2PVc6OzRzPD;hQi=>
CV1	MVLGeY5kfGmxbjDhd5NigQ>?			M4P1cGFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBqdiCFVkGgZ4VtdHNuIFXDOVAhRSByLkOwPEDPxE1w	Mn\rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdyMEWzPVQoRjF5MEC1N\|k1RC:jPh?=
Huh7	NETSW4dHfW6ldHnvckBie3OjeR?=			Ml7GRYN1cX[rdImgZZQhcHWvYX6gVHBCWiCpYX3tZUBqdiCKdXi3JINmdGy\|IHL5JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMkKg{txONg>?	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzB\|NEG0PUc,OTdyM{SxOFk9N2F-
CV1	NEe1O4NHfW6ldHnvckBie3OjeR?=			M3;6TWFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIFPWNUBk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkOwPEDPxE1w	NXPzfVFpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexNlk4OjVpPkG3NVI6PzJ3PD;hQi=>
HepG2	M4TJVWZ2dmO2aX;uJIF{e2G7			NF3IOplC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBqdiCKZYDHNkBk\WyuczDifUBRWEGULVfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6wPFIh\|ryPLh?=	NIr3e3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G1O\|AyQSd-MUexOVcxOTl:L3G+
HEK293	M{HIb2Z2dmO2aX;uJIF{e2G7			MVPB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIJ6KEeDTESgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yM{Wg{txONg>?	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzJ7Mk[wOkc,OTd{OUK2NFY9N2F-
HEK293	Mmn6SpVv[3Srb36gZZN{[Xl?			NWPBWYZJSWO2aY\peJkh[XRiaIXtZY4h[WSrcH;z[UB1cXO\|dXWgVHBCWiCpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIJ6KFCSQWKtS2FNPCC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlMyKM7:TT6=	M2fUOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{SzN\|cyLz5zN{O0N\|M4OTxxYU6=
L6	NUf5ZYgzTnWwY4Tpc44h[XO\|YYm=			NVPB[ppjTW[oZXP0JI9vKG[jdIT5JIFkcWRib4jp[IF1cW:wIHnuJJJifCCONjDj[YxteyxiRVO1NEA:KDVizszNMi=>	MofHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd\|NEOzO\|EoRjF5M{SzN\|cyRC:jPh?=
HepG2/C3A	NFrOdZJHfW6ldHnvckBie3OjeR?=			MYnBZ5RqfmG2aX;uJI9nKGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5vbXXkbYF1\WRidILhcpNkemmydHnvcoFtKGGldHn2bZR6KGmwIHj1cYFvKEincFeyM2M{SSClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKG[3c3XkJGdidDRvTFLEJIJ6KGOxdILhcpNn\WO2aX;uJIF{e2G7LDDFR\|UxKD1iMD6wNFM5KM7:TT6=	MkTiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MEO2PFgoRjF5NECzOlg5RC:jPh?=
CV1	NH;oV5dHfW6ldHnvckBie3OjeR?=		NI[0[FEzPCCqcoO=	MofjRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgbY4hS1ZzIHPlcIx{KGK7IFfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7IHHmeIVzKDJ2IHjyd{whTUN3MDC9JFAvODNizszNMi=>	M{PrUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUC3NlI2Lz5zN{WwO\|IzPTxxYU6=
CV1	NF\vS4tHfW6ldHnvckBie3OjeR?=		MYiyOEBpenN?	NV65bW82SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUYXzwbIEhcW5iQ2[xJINmdGy\|IHL5JGdCVDRidILhcpNi[3SrdnH0bY9vKGG\|c3H5JIFnfGW{IEK0JIhzeyxiRVO1NEA:KDNwNE[g{txONg>?	NUHteZJ{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NFczOjVpPkG3OVA4OjJ3PD;hQi=>
NIH3T3	NE[5PIVHfW6ldHnvckBie3OjeR?=			MU\B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO\|ZXSgbY4hVkmKM2SzJINmdGy\|IHL5JGdCVDRidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gNE4{OiEQvF2u	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ{NEe3O{c,OTd4MkS3O\|c9N2F-
HEK293	MWjGeY5kfGmxbjDhd5NigQ>?			NGqwW21C\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHHQNkBo\W6nIHnu[JVkfGmxbjygSWM2OCB;IECuNVIh\|ryPLh?=	NFvMO3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOECyPVE4Pid-MUiwNlkyPzZ:L3G+
HEK293	NHeySZlHfW6ldHnvckBie3OjeR?=			MkTFRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{BjgSCJQVy0JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMUmg{txONg>?	NX;wfYNjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiwNlkyPzZpPkG4NFI6OTd4PD;hQi=>
CV1	M3LFZmZ2dmO2aX;uJIF{e2G7			MV;B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO\|ZXSgbY4hS1ZzIHPlcIx{KGK7IILlZ4VxfG:{IITyZY5{[WO2aY\heIlwdiCjc4PhfUwhTUN3MDC9JFAvOzB6IN88UU4>	M4jtVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MEK5NVc5Lz5zOECyPVE4QDxxYU6=
HEK293	NFrsPJNHfW6ldHnvckBie3OjeR?=			NHvqUotC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCrbjDISWszQTNiY3XscJMh[nliR1HMOEB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkC0OUDPxE1w	M4HaeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkmxOlQ2Lz5zOEK5NVY1PTxxYU6=
U2OS	NU\1fHRqTnWwY4Tpc44h[XO\|YYm=			M2PDc2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIlvKFV{T2OgZ4VtdHNiYomgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMjFOwG0v	M{nteVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{K5O\|UyLz5zOEOyPVc2OTxxYU6=
HepG2	MlPwSpVv[3Srb36gZZN{[Xl?			M2LTfmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCKZYDHNkBk\WyuczDifUBISUx2IITyZY5{[WO2aY\heIlwdiCjc4PhfUwhTUN3MDC9JFAvOjJ\|IN88UU4>	NUPlVY4xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizPVQ6ODdpPkG4N\|k1QTB5PD;hQi=>
HepG2	M1jGdmZ2dmO2aX;uJIF{e2G7			MWDB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKHKnY3XweI9zKHS{YX7zZYN1cX[jdHnvckBjgSC{ZYDvdpRmeiCpZX7lJIF{e2G7IILlcIF1cX[nIITvJINwdnS{b3ysJGVEPTBiPTCwMlA2KM7:TT6=	NG\6ZVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[yOVU2QSd-MUi2NlU2PTl:L3G+
RAW264.7	NUC4W\|ZbTnWwY4Tpc44h[XO\|YYm=			M2\1fHN2eHC{ZYPzbY9vKG:oIFzQV{9KTk5vZ3HtcYEue3SrbYXsZZRm\CCQTzDwdo9lfWO2aX;uJIlvKG2xdYPlJHJCXzJ4ND63JINmdGy\|LDDJR\|UxKD1iMUeuOUDPxE1w	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDhyOUOyOUc,OTh6MEmzNlU9N2F-
CV1	NH\GUpBHfW6ldHnvckBie3OjeR?=			MmfrRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDh[pJq[2GwIHfy[YVvKG2xbnvlfUBEXjFiY3XscJMh[29vdILhcpNn\WO2ZXSge4l1cCCodYPl[EBI[Wx2LVTCSEBjgSC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlA{OyEQvF2u	NV;q[Zh{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4NlYzODVpPkG4PFI3OjB3PD;hQi=>
CV1	MojFSpVv[3Srb36gZZN{[Xl?			M1ToWmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmGucHjhJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS1ZzIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiZoXz[YQhT2GuND3ERmQh[nlidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gN{41PiEQvF2u	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDh{NkKwOUc,OTh6Mk[yNFU9N2F-
HepG2	NGj4eoNHfW6ldHnvckBie3OjeR?=			MXnB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJidHCqYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIHj1cYFvKEincFeyJINmdGy\|IHPvMZRz[W6\|ZnXjeIVlKHerdHigS4FtPCCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjB\|OTFOwG0v	NGHnWHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEizOVcyQSd-MUi4N\|U4OTl:L3G+
HepG2	M4nxO2Z2dmO2aX;uJIF{e2G7			NH;hUo9C\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJIh2dWGwIFjldEBIOiClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKEejbEStSGJFKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODRizszNMi=>	NFrKVpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC1N\|c4Pid-MUmwOVM4PzZ:L3G+
HeLa	NWrG[mdjTnWwY4Tpc44h[XO\|YYm=			MVLB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJIh2dWGwIFjlUIEh[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBI[Wx2LVTCSEBie3Onc4Pl[EBieyC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCkeTDHZYw1KHKnc4DvcpNmKGWuZX3lcpQu\HKrdnXuJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMEG1JO69VS5?	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTJ5NUm2N{c,OTl{N{W5OlM9N2F-
BL21	NVP3ZVFMTnWwY4Tpc44h[XO\|YYm=			M1vhfGRqe3CuYXPlcYVvfCCxZjDyZYRqdyCuYXLlcIVlKDJqUzmtLFIu[mWwen;5cE1xcGWweXzhcYlvdylvMz37OE1cOSxzLXTpeJJqfGmxLUKtLFUudWW2aInsMVIueGinbonsMY95[XqxbD20MZltMS2ndHjvfJleNXCqZX75cJ0ueHKxcHnvcolkKGGlaXSg[pJwdSCJU2St[pV{\WRiaIXtZY4hWFCDUnfhcY1iKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEh[2WubIOgZpkhe2OrboTpcIxifGmxbjDwdo95cW2rdImgZZN{[XluIFnDOVAhRSByLkS1JO69VS5?	NH;HWGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO0PVE4Pid-MUmzOFkyPzZ:L3G+
BHK21	NHHyeWhHfW6ldHnvckBie3OjeR?=			NWjsUIk3SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFLIT\|IyKGOnbHzzJIF{e2W\|c3XkJIF{KFOHQWCgZYN1cX[rdImgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlQ2KM7:TT6=	NGDHNFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO0PVE4Pid-MUmzOFkyPzZ:L3G+
COS1	M2rW[GZ2dmO2aX;uJIF{e2G7		NGC2SZg1QCCqcoO=	MYnB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIILlZ49u[mmwYX70JHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iQ1;TNUBk\WyuczDjc{1mgHC{ZYPzbY5oKEeDTESgZZN{\XO\|ZXSgZZMhfHKjboPjdolxfGmxbnHsJIFkfGm4aYT5JIFnfGW{IES4JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjB{IN88UU4>	NVXITVRORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1NFc5PjFpPkG5OVA4QDZzPD;hQi=>
COS1	MkH1SpVv[3Srb36gZZN{[Xl?			NF7TSpdC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iYX\ybYNidiCpcnXlckBud26tZYmgR29UOSClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKG[3c3XkJJlm[XO2IFfhcFQuTEKGIHL5JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEKg{txONg>?	NHG1dpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWzNFY5OSd-MUm1N\|A3QDF:L3G+
U2OS	M4TGPGZ2dmO2aX;uJIF{e2G7			MYrB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO\|ZXSgbY4hXTKRUzDj[YxteyCkeTDseYNq\mW{YYPlJJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEWg{txONg>?	NUeyb3o4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1O\|QxPTZpPkG5OVc1ODV4PD;hQi=>
MCF7	M1\iOWZ2dmO2aX;uJIF{e2G7	MVuwMlEhfU1?	MmfuNlQhcHK\|	NHqwRoxC[3SrdnH0bY9vKG:oIHj1cYFvKFCSQWKt[4FudWFvZHXw[Y5l\W62IITyZY5{[3KrcITpc44h\XiycnXzd4VlKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKFCSUlWtdoVxd3K2ZYKg[4Vv\SCneIDy[ZN{cW:wIHH0JFAvOSC3TTDh[pRmeiB{NDDodpMh[nliZIXhcEBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XlicnXsZZRqfmVidH:gdo9{cWeuaYThfo9v\Q>?	NHXPT\|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUexPVI{Pid-MUm3NVkzOzZ:L3G+
HepG2	MoD6SpVv[3Srb36gZZN{[Xl?			MnvHRYdwdmm\|dDDhZ5Rqfmm2eTDheEBISUx2LYTh[4dm\CCqdX3hckBRWEGUZ3HtcYEhdGmpYX7kJIJqdmSrbneg[I9u[WmwIHX4dJJme3OnZDDpckBpfW2jbjDI[ZBIOiClZXzsd{Bie3Onc4Pl[EBieyC{ZXPldJRweiC2cnHud4FkfGm4YYTpc44h[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4xOzlizszNMi=>	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd5NUG2PUc,OTl5N{WxOlk9N2F-
HepG2	M3n2TmZ2dmO2aX;uJIF{e2G7			NUK1eo1qSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjldGczKGOnbHzzJIJ6KEeDTESgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5{MkOg{txONg>?	NGT3[5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUe4N\|Q1PCd-MUm3PFM1PDR:L3G+
COS7	M17RN2Z2dmO2aX;uJIF{e2G7			NHXZS2pC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJJJm[2:vYnnuZY51KFCSQWLnZY1u[TFiTFLEJIV5eHKnc4Pl[EBqdiCjZoLpZ4FvKGe{ZXXuJI1wdmuneTDDU3M4KGOnbHzzJINw\XiycnXzd4lv\yCJQVy0JIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkCzJO69VS5?	M2\SfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUO4O\|YzLz5{MEGzPFc3OjxxYU6=
COS7	M{LhO2Z2dmO2aX;uJIF{e2G7			NGLJfllC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJJJm[2:vYnnuZY51KFCSQWLnZY1u[TJiTFLEJIV5eHKnc4Pl[EBqdiCjZoLpZ4FvKGe{ZXXuJI1wdmuneTDDU3M4KGOnbHzzJINw\XiycnXzd4lv\yCJQVy0JIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkGg{txONg>?	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF\|OEe2Nkc,OjBzM{i3OlI9N2F-
HEK	NGLkTlFHfW6ldHnvckBie3OjeR?=			MWfB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFfBUFQufGGpZ3XkJHBRSVKpYX3tZUBkcGmvZYLpZ{Bz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLJINmdGy\|IHL5JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEO1JO69VS5?	NHrlW2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEK5PVIyPCd-MkCyPVkzOTR:L3G+
HEK	M1vKbWZ2dmO2aX;uJIF{e2G7			MX3B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFfBUFQufGGpZ3XkJHBRSVKjbIDoZUBkcGmvZYLpZ{Bz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLJINmdGy\|IHL5JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDFyLkW4JO69VS5?	NGiwVHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEK5PVIyPCd-MkCyPVkzOTR:L3G+
CHO-K1	NFnzN2pHfW6ldHnvckBie3OjeR?=			NHPlfpFR[XK2aXHsJIFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hMWxDTCCneIDy[ZN{\WRiaX6gR2hQNUtzIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiR1HMOEBie3Onc4Pl[EBieyCudXPp[oVz[XOnIHHjeIl3cXS7IHL5JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMTFOwG0v	NUDIN3JiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1Nlc6PjlpPkKwOVI4QTZ7PD;hQi=>
3T3L1	MWjGeY5kfGmxbjDhd5NigQ>?		MWK3JIRigXN?	NVT1WYs5UW6mdXP0bY9vKG:oIHHkbZBw\2WwZYPpd{BqdiCvb4Xz[UA{XDOOMTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBtcXCrZDDhZ4N2dXWuYYTpc44h[W[2ZYKgO{Bl[Xm\|IHL5JG9qdCC{ZXSgU{B{fGGrbnnu[y=>	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODV{N{m2PUc,OjB3Mke5Olk9N2F-
HEK293T	M2LO[WZ2dmO2aX;uJIF{e2G7	M2nwXFEhfU1?	M2XsUVI1KGi{cx?=	MUfQZZJ1cWGuIHHnc45qe3RiYXP0bZZqfHliYYSgbJVu[W5iUGDBVodidW2jLVzCSEBmgHC{ZYPz[YQhcW5iSFXLNlk{XCClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gdoVk\XC2b4KgeJJidnOjY4TpeoF1cW:wIHH0JFEhfU1iYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeR?=	M{Hz[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEOwNlY{Lz5{MUCzNFI3OzxxYU6=
CV1	Mof5SpVv[3Srb36gZZN{[Xl?			NXi1Opp4XHKjboPhZ5RqfmG2aX;uJI9nKEejbESt[pV{\WRiaIXtZY4hWFCDUnfhcY1iKESQQTDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS1ZzIHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvOSEQvF2u	M{\5fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUGyO\|g1Lz5{MUGxNlc5PDxxYU6=
HEK293	M1rWd2Z2dmO2aX;uJIF{e2G7		MnHoNVYhfG9iMkCgbJJ{	M4fJTXRz[W6\|YXP0bZZifGmxbjDv[kBI[Wx2LX\1d4VlKGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIFnfGW{IEG2JJRwKDJyIHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlA1OyEQvF2u	MkLtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzMki2NFAoRjJzMUK4OlAxRC:jPh?=
HepG2	NWr0W\|VVTnWwY4Tpc44h[XO\|YYm=	MXOxNEB2dW:uL1y=	M3PGb\|I1KGi{cx?=	M3W2[2lv[3KnYYPlJIlvKGeudXPvd4Uh[2:wc4XtdJRqd25iaX6gbJVu[W5iSHXwS\|Ih[2WubIOgZZQhOTBidX3vcE9NKGGodHXyJFI1KGi{czDy[YxifGm4ZTD0c{Bkd262cn;s	M2fCUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkW2O\|Q5Lz5{MUK1Olc1QDxxYU6=
HepG2	NGfRVJFHfW6ldHnvckBie3OjeR?=			MmXVWJJidnOjY4TpeoF1cW:wIH;mJIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO\|ZXSgbY4hcHWvYX6gTIVxTzJiY3XscJMh[29vdILhcpNn\WO2ZXSge4l1cCCSUGLFN{1VUy2OdXOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxKD1iMD6wOUDPxE1w	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR3MES2PEc,OjF2NUC0Olg9N2F-
HepG2	MYjGeY5kfGmxbjDhd5NigQ>?			MojFRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIHj1cYFvKEincFeyJINmdGy\|IHL5JHBRWkVvbIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4xOSEQvF2u	M3rpPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEiyOFQ3Lz5{MUS4NlQ1PjxxYU6=
CV1	NIS2bIdHfW6ldHnvckBie3OjeR?=		NGjyeHE1OCCqcoO=	MlPDRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGtUGJFKGW6cILld5Nm\CCrbjDDWlEh[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBI[Wx2IHHmeIVzKDRyIHjyd{BjgSCudXPp[oVz[XOnIHLhd4VlKHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNUDPxE1w	MmPNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUWwOlMoRjJzNUG1NFY{RC:jPh?=
3T3L1	NXm2PHl1TnWwY4Tpc44h[XO\|YYm=	M2fSXlExKHWP		NHfQN4RKdmO{ZXHz[UBqdiCjZHnwc45m[3SrbjDtVm5CKGyndnXsd{BqdiCWTl\hcJBp[S2rbnT1Z4VlKG2xdYPlJFNVO0xzIHPlcIx{KHC{ZYTy[YF1\WRiYYSgNVAhfU1iYnXmc5JmKFSQRj3hcJBp[SClaHHscIVv\2VicnXsZZRqfmVidH:gZ49vfHKxbB?=	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh3MUC4PUc,OjF6NUGwPFk9N2F-
COS7	NI\NRXZHfW6ldHnvckBie3OjeR?=			NWLQRW4{VW:mdXzheIlwdiCxZjDoeY1idiCSUFHS[4FudWFvTFLEJIV5eHKnc4Pl[EBqdiCjZoLpZ4FvKGe{ZXXuJI1wdmuneTDDU3M4KGOnbHzzJINwNXS{YX7z[oVkfGWmIIfpeIghT2GuNDDhd5Nme3OnZDDhd{Bi[3SrdnH0bY9vKG:oIITyZY5{[WO2aY\heIlwdiCjY4Tpeol1gSCkeTDseYNq\mW{YYPlJIF{e2G7LDDFR\|UxKD1iMD6wOFMh\|ryPLh?=	MmHYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{OwO\|AoRjJzOEezNFcxRC:jPh?=
Sf21	MWXGeY5kfGmxbjDhd5NigQ>?			MkjBTY5pcWKrdHnvckBw\iCqdX3hckBDW0WSIHX4dJJme3OnZDDpckBxdGG\|bXGgcYVu[nKjbnWgeoV{cWOuZYOgc4YhW2Z{MTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEGWUD3k[ZBmdmSnboSgX\|NJZXSjdYLvZ4hwdGG2ZTD1dJRic2VuIFnDOVAhRSB4LkSg{txONg>?	M4rVe\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOU[1OlI{Lz5{MUm2OVYzOzxxYU6=
Sf21	MVrGeY5kfGmxbjDhd5NigQ>?			NYi2PJBlUW6qaXLpeIlwdiCxZjDTdJJi\3WnLVThe4xmgSC{YYSgRpNmeCCneIDy[ZN{\WRiaX6gdIxie22jIH3lcYJz[W6nII\ld4lkdGW\|IH;mJHNnOjFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBCXFBvZHXw[Y5l\W62IGuzTH11[XW{b3Poc4xifGVidYD0ZYtmNCCLQ{WwJF0hPDBizszNMi=>	NV3UWnVuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5OlU3OjNpPkKxPVY2PjJ\|PD;hQi=>
COS-1	NHr4RYxHfW6ldHnvckBie3OjeR?=		MUKyOEBpenN?	MXjB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIFPPV{0yKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmVvYnHz[YQhdHWvaX7vcYV1emmlIHHuZYx6e2m\|LDDFR\|UxKD1iMD6wOFgh\|ryPLh?=	M1HrdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEWxNFU1Lz5{MkC1NVA2PDxxYU6=
COS1	NHOyeppHfW6ldHnvckBie3OjeR?=			M3LrVGJqdmSrbnegZYZncW6rdImgeI8hcHWvYX6ge4lt\CC2eYDlJHBRSVKpYX3tZUBNSkRiZYjwdoV{e2WmIHnuJGNQWzFiY3XscJMh[29vZYjwdoV{e2mwZzDHRWw1KGK7IIPjbY51cWyuYYTpc44heHKxeHntbZR6KGG\|c3H5	M3zOVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEewOlA1Lz5{MkC3NFYxPDxxYU6=
HepG2	M4\zVmZ2dmO2aX;uJIF{e2G7		MWKyNEBpenN?	MmOyRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDHRWw1NW[3c3XkJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJGhmeEd{IHPlcIx{KGGodHXyJFIxKGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMjFOwG0v	NGrpbHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC4NVk{Oid-MkKwPFE6OzJ:L3G+
BL21 DE3	NGTjR4VHfW6ldHnvckBie3OjeR?=			MoLySIl{eGyjY3Xt[Y51KG:oIGuzTH1zd3OrZ3zpeIF7d26nIH\yc40hVi22ZYLtbY5idCCKaYOteIFo\2WmIHj1cYFvKFCSQWLnZY1u[SCuaXfhcoQh[mmwZHnu[{Bld22jaX6g[ZhxemW\|c3XkJIlvKEW\|Y3jldolkcGmjIHPvcIkhSkx{MTDESVMh[2WubIOgZpkhe2OrboTpcIxifGmxbjDwdo95cW2rdImgZZN{[XluIFvpJF0hOC5yN{Sg{txONg>?	M4qxcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEixPVMzLz5{MkC4NVk{OjxxYU6=
COS1	NXXaZ24{TnWwY4Tpc44h[XO\|YYm=		NWD2T\|ZpOjRiaILz	NFTRUoVVemGwc3HjeIl3[XSrb36gc4YhcHWvYX6g[pVtdCCuZX7neIghWFCDUnfhcY1iKGW6cILld5Nm\CCrbjDDU3MyKGOnbHzzJINwNXS{YX7z[oVkfGWmIIfpeIghWliUYXzwbIEh[W[2ZYKgNlQhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMUKg{txONg>?	MlXIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzOUezPVYoRjJ{MUm3N\|k3RC:jPh?=
MG-63	NWDvZ4Q1TnWwY4Tpc44h[XO\|YYm=			NGDpOWlC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iTVetOlMh[2WubIOgZpkhemWyb4L0[ZIh\2WwZT3iZZNm\CC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlAyOSEQvF2u	NWrINVVJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyNlU3PDFpPkKyNlI2PjRzPD;hQi=>
293H DA	NU\2cWF{TnWwY4Tpc44h[XO\|YYm=		Mnz2NVYhcHK\|	MoXQRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhVEKGIHnuJIh2dWGwIEK5N2ghTEFiY3XscJMh[W[2ZYKgNVYhcHK\|IHL5JHRTNU[URWSgZYN1cX[jdHnvckBz\XCxcoTldkBie3OjeTygSWM2OCB;IECuNFAzPCEQvF2u	NF35TpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW4Nlk4Oyd-MkK1PFI6PzN:L3G+
MG-63	M1HIR2Z2dmO2aX;uJIF{e2G7		MnP6NlQhcHK\|	MVnNc4R2dGG2aX;uJI9nKG[3bHytcIVv\3SqIHj1cYFvKHCVR{Wt[pV{\WRiUGDBVodidW2jIHX4dJJme3OnZDDpckBOTy14MzDj[YxteyClbz3lfJBz\XO\|aX7nJJBIXi2SMj3QVHJGKGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZoF{\WRidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gNE4xOTFizszNMi=>	M3LKc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{K3OFQ5Lz5{MkeyO\|Q1QDxxYU6=
Ac2F	NEflVXlHfW6ldHnvckBie3OjeR?=	MXWwMlEhfG9iMUCgeW0>	MlnjOkBpenN?	Ml36RYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhcW5icnH0JGFkOkZiY3XscJMh[XO\|ZYPz[YQh[XNibIXjbYZmemG\|ZTDhZ5Rqfmm2eTDheEAxNjFidH:gNVAhfU1iYX\0[ZIhPiCqcoOgZpkhemWyb4L0[ZIh\2WwZTDhd5NigQ>?	NE\sbVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkixPVE6OCd-MkK4NVkyQTB:L3G+
HepG2	MVXGeY5kfGmxbjDhd5NigQ>?			MnT4WJJidnOjY4TpeoF1cW:wIH;mJGdCVDRvZoXz[YQhWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjD0doFve2[nY4Tl[EBqdiCqdX3hckBJ\XCJMjDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjFizszNMi=>	NGW1T5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmzOFU{Pyd-MkK5N\|Q2Ozd:L3G+
THP1	Ml;QSpVv[3Srb36gZZN{[Xl?	M{TETlExOCC3TR?=	NUn6N29xOyCqcoO=	M2Ljd3Rz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5iaIXtZY4hXEiSMTDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG\|ZTDpckBNWFNvaX7keYNm\CCPQ2CxJI1TVkFiZYjwdoV{e2mxbjDheEAyODBidV2gdJJmcW6ldXLheIVlKG[xcjCzJIhzeyCycnnvdkB1dyCOUGOgZ4hidGynbnflJI1m[XO3cnXkJIFnfGW{IEG4JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXNicnXsZZRqfmVidH:geY51emWjdHXkJINwdnS{b3y=	M1rSV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUO0OVM4Lz5{MkmzOFU{PzxxYU6=
THP1	M{\XbmZ2dmO2aX;uJIF{e2G7	NITE[ngyODBidV2=	NY\M[Y5xOyCqcoO=	NV;mZWlHXHKjboPhZ5RqfmG2aX;uJI9nKFCSQWLnZY1u[SCrbjDoeY1idiCWSGCxJINmdGy\|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKEySUz3pcoR2[2WmIFnMOkBuWk6DIHX4dJJme3Orb36gZZQhOTByIIXNJJBz\WmwY4XiZZRm\CCob4KgN{BpenNicILpc5IhfG9iTGDTJINp[WyuZX7n[UBu\WG\|dYLl[EBi\nSncjCxPEBpenNiYomgVnQuWEOUIHHuZYx6e2m\|IILlcIF1cX[nIITvJJVvfHKnYYTl[EBkd262cn;s	NITVUoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmzOFU{Pyd-MkK5N\|Q2Ozd:L3G+
HepG2	M1nR[2Z2dmO2aX;uJIF{e2G7	Mn;HNVAhfU1?	MV:yOEBpenN?	MWLS[YR2[3Srb36gc4Yh\2y3Y3;z[UBkd26\|dX3weIlwdiCrbjDpcpN2dGmwLYLld4l{fGGwdDDoeY1idiCKZYDHNkBk\WyuczDheEAyOCC3TTDh[pRmeiB{NDDodpMh[nliZ3z1Z49{\SCxeHnkZZNmKG2ndHjv[EBqdiCycnXz[Y5k\SCxZjCwMlEhfU1ib3[gbY5{fWyrbh?=	NYrJW2g{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNlUzPDRpPkKzNFI2OjR2PD;hQi=>
HepG2	M2\BbmZ2dmO2aX;uJIF{e2G7	MmDlNVAhfU1?	NWeydWROOjRiaILz	NV3XfoxLWmWmdXP0bY9vKG:oIHfseYNwe2ViY3;ud5VueHSrb36gbY4hcW6\|dXzpck1z\XOrc4ThcpQhcHWvYX6gTIVxTzJiY3XscJMh[XRiMUCgeW0h[W[2ZYKgNlQhcHK\|IHL5JIdtfWOxc3Wgc5hq\GG\|ZTDt[ZRpd2RiaX6gdJJme2WwY3Wgc4YhOjJwMjDtUUBw\iCpbIXjc5Nm	M1zJRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEK1NlQ1Lz5{M{CyOVI1PDxxYU6=
HepG2	MYfGeY5kfGmxbjDhd5NigQ>?		M4HpfVIxKGi{cx?=	NUniPIZHXHKjboPhZ5RqfmG2aX;uJI9nKEeDTESt[pV{\WRiaIXtZY4hWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO\|ZXSgbY4hUGWyR{KgZ4VtdHNiYX\0[ZIhOjBiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFM6KM7:TT6=	NFuy[Gg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G3NVA1PSd-MkOxO\|ExPDV:L3G+
L02	NXzQSHZHTnWwY4Tpc44h[XO\|YYm=		M{nZNVI1KGi{cx?=	M3noPWFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iNUyERDDlfJBz\XO\|ZXSgbY4hcHWvYX6gUFAzKGOnbHzzJINwNWW6cILld5NqdmdicFfMN{1UXjRyLVfBUFQh[W[2ZYKgNlQhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDiZZNm\CC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlkh\|ryPLh?=	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF6NkOwO{c,OjNzOE[zNFc9N2F-
HEK293	M1;4fmZ2dmO2aX;uJIF{e2G7		M{KyUFQ5KGi{cx?=	MkDpRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDjc5Rz[W6\|ZnXjeIVlKHerdHigVHBTTXh2LWTLMYx2[yCjc4Pld5Nm\CCjczDhZ5RqfmG2aX;uJI9nKGy3Y3nm[ZJie2ViYXP0bZZqfHlibXXhd5Vz\WRiYX\0[ZIhPDhiaILzJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IESzMlcyKM7:TT6=	NX3lS\|dZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOlU5PDRpPkKzNlY2QDR2PD;hQi=>
HEK293	NGO4RXRHfW6ldHnvckBie3OjeR?=	M2nPeVExKHWP		NXHJPXBRXHKjboPhZ5RqfmG2aX;uJI9nKFCSQWLnZY1u[SBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[29vdILhcpNn\WO2ZXSge4l1cCCDUEKtVHBTTSCjdDCxNEB2VSCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk>	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ5M{WxPUc,OjN{N{O1NVk9N2F-
HEK293	NWrvSJlqTnWwY4Tpc44h[XO\|YYm=			NH;ER\|dC\2:waYP0JIF1KGi3bXHuJHBRSVKpYX3tZUBNSkRiZYjwdoV{e2WmIHnuJIh2dWGwIFjFT\|I6OyClZXzsd{Bkd3S{YX7z[oVkfGWmIIfpeIghT0GOND3MeYMh[XO\|ZYPz[YQh[XNidILhcpNkemmydHnvcoFtKGGldHn2ZZRqd25iYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yMESg{txONg>?	NV3hR41ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyPFY4QDdpPkKzNlg3Pzh5PD;hQi=>
HEK293	NXnscXRKTnWwY4Tpc44h[XO\|YYm=		M37DT\|I1KGi{cx?=	M2X1enRz[W6\|YXP0bZZifGmxbjDv[kBISUx2IFTCSE1nfXOnZDDoeY1idiCSUFHS[4FudWFvTFLEJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvOSEQvF2u	MkHBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OUS4N\|AoRjJ\|Mkm0PFMxRC:jPh?=
CMT93	M{DPeGZ2dmO2aX;uJIF{e2G7	MV6xNEB2VQ>?	M4nMflMxKG2rboO=	NYHkVIJ[UW6mdXP0bY9vKG:oIGDQRXIu\2GvbXGgdJJwdW:2ZYKgZYN1cX[rdImgbY4hVFCVLYP0bY12dGG2ZXSgcY92e2ViQ13UPVMh[2WubIOgZZQhOTBidV2gdJJmcW6ldXLheIVlKG[xcjCzNEBucW6\|IHLl[o9z\SCOUGOtZ4hidGynbnflJI1m[XO3cnXkJIFnfGW{IE[gbJJ{KHCxc4SgZ4hidGynbnflJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeR?=	NWDhRVY1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NVYyQTBpPkKzOFE3OTlyPD;hQi=>
HepG2	NXvwe4V1TnWwY4Tpc44h[XO\|YYm=			NETDU\|JC\2:waYP0JIFkfGm4aYT5JIF1KEeDTESt[pV{\WRiUGDBVodidW2jIF20OlNCKG23dHHueEApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\XCJMjDj[YxteyCkeTD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjByMjFOwG0v	NILtNWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WwNlIyOid-MkO1NFIzOTJ:L3G+
HepG2	M1vmcGZ2dmO2aX;uJIF{e2G7			MVfB[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRvZoXz[YQhWFCDUnfhcY1iKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIHj1cYFvKEincFeyJINmdGy\|IHL5JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEGg{txONg>?	M4\MbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NUCyNlEzLz5{M{WwNlIyOjxxYU6=
L6	Mlf1SpVv[3Srb36gZZN{[Xl?		MVSyOEBpenN?	MVPJcoR2[3Srb36gc4Yh\2y3Y3;z[UB2eHSja3WgbY4hemG2IFy2JINmdGy\|IID1cJNm\CC5aYToJGMyPC2mZX;4fUBodHWlb4PlJIFnfGW{IEK0JIhzeyCrbjDwdoV{\W6lZTDv[kBqdnO3bHnuMEBGSzVyIE2gOE41QSEQvF2u	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl7Mki2Nkc,OjN7OUK4OlI9N2F-
L6	NX7FV5dvTnWwY4Tpc44h[XO\|YYm=		M1:zPFE3KGi{cx?=	NF7tNGNKdmO{ZXHz[UBqdiB{LWuzTH0u\GWxeInncJVkd3OnIIXweIFs\SCrbjDyZZQhVDZiY3XscJMh[W[2ZYKgNVYhcHK\|IHL5JJNkcW62aXzsZZRqd25iY3;1cpRqdmdiYX7hcJl{cXNuIFnDOVAhRSB2Lkig{txONg>?	NV6zOWJIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVM4OzhpPkK0PFE{PzN6PD;hQi=>
HEK293	MXHGeY5kfGmxbjDhd5NigQ>?	NWq4UYFlOTBidV2=	MYiyOEBpenN?	NIjvPYJVemGwc3HjeIl3[XSrb36gc4YhWFCDUj3nZY1u[SBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XRiMUCgeW0h[W[2ZYKgNlQhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigQ>?	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh7MEC5NEc,OjR6OUCwPVA9N2F-
HEK293	M{TRZ2Z2dmO2aX;uJIF{e2G7		NHHWXXMzPCCqcoO=	NYfGTHBESWexbnnzeEBi[3Srdnn0fUBifCCvb4Xz[UBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{Bkdy2neIDy[ZN{cW6pIIfpeIghT2GuNDDy[ZBwenSncjD2[YN1d3JiYX\0[ZIhOjRiaILzJIJ6KGS3YXytcJVkcW[ncnHz[UBz\XCxcoTldkBie3OjeTygSWM2OCB;IECuNFMh\|ryPLh?=	MlWwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NUW4PFkoRjJ2OUW1PFg6RC:jPh?=
DU145	NIS1fZlEgXSxdH;4bYNqfHliYYPzZZk>		NETZTo41QCCqcoO=	NFq4b\|FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBFXTF2NTDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxKD1iMU[g{txONg>?	NGjzVmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm5OlE1Oyd-MkS5PVYyPDN:L3G+
PC3	NX\0RopuS3m2b4TvfIlkcXS7IHHzd4F6		NEjmUpQ1QCCqcoO=	M1XCRmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBEOyClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOjBwMzFOwG0v	NFvZd4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm5OlE1Oyd-MkS5PVYyPDN:L3G+
MDA-MB-231	NEHCWmxIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MV:yOEBpenN?	MULHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNSGEuVUJvMkOxJINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB3LkKzJO69VS5?	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ5OEKzOkc,OjV{N{iyN\|Y9N2F-
HEK293	MkfoSpVv[3Srb36gZZN{[Xl?			NHPLTJhC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFEh\|ryPLh?=	MkT2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV\|MEW2PFgoRjJ3M{C1Olg5RC:jPh?=
HepG2	NICyUoRHfW6ldHnvckBie3OjeR?=		NETwbVAzOCCqcoO=	M1;pXHRz[W6\|YXP0bZZifGmxbjDv[kBRWEGUIITyZY5{\mWldHXkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFIxKGi{czDifUBRWEGULXz1Z4ln\XKjc3WgZZN{[XluIFXDOVAhRSBzLk[g{txONg>?	NGPpRVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzO\|MxPCd-MkW0N\|c{ODR:L3G+
HEK293	MWnGeY5kfGmxbjDhd5NigQ>?		MV6yOEBpenN?	NUT3U5NDXHKjboPhZ5RqfmG2aX;uJI9nKFCSQWLnZY1u[SBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMhcW6ldXLheIVlKG[xcjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6	NHrJRo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS0NlMzOid-MkW0OFI{OjJ:L3G+
HepG2	MV3GeY5kfGmxbjDhd5NigQ>?			M{ToTmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gS2FNPC2SUFHS[4FudWFibHnnZY5lKGKrbnTpcoch\G:vYXnuJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\XCJMjDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjB\|OTFOwG0v	MkT3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NkKyPFEoRjJ3NE[yNlgyRC:jPh?=
HepG2	MkLtSpVv[3Srb36gZZN{[Xl?		MV[yNEBpenN?	Mkm5RYdwdmm\|dDDhZ5Rqfmm2eTDheEBISUx2LVTORUBjcW6maX7nJIRwdWGrbjDmeZNm\CCqdX3hckBRWEGUZ3HtcYEhdGmpYX7kJIJqdmSrbneg[I9u[WmwIHX4dJJme3OnZDDpckBpfW2jbjDI[ZBIOiClZXzsd{Bie3Onc4Pl[EBieyC{ZXPldJRweiC2cnHud4FkfGm4YYTpc44hcW6ldXLheIVlKG[xcjCyNEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yNDFOwG0v	M4Xhc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEm3NVMzLz5{NUS5O\|E{OjxxYU6=
HepaR	NEX4c3hHfW6ldHnvckBie3OjeR?=	MWmxJJVO	NHX1[\|UyKGSjeR?=	MV7B[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCqdX3hckBJ\XCjUjDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBHSUKSMTDn[Y5mKGW6cILld5Nqd25iYYSgNUB2VSCrbnP1ZoF1\WRiZn;yJFEh\GG7IHL5JJF2[W62aYTheIl3\SCSQ2KgcYV1cG:mIILlcIF1cX[nIITvJJVvfHKnYYTl[EBkd262cn;s	MkfZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUexN\|IoRjJ3NEm3NVMzRC:jPh?=
HepaR	MUHGeY5kfGmxbjDhd5NigQ>?	NEnVPHEyKHWP	NXL6coR3OSCmYYm=	NEnF[2hC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCrbjDoeY1idiCKZYDhVkBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCDTlfQWGw1KGenbnWg[ZhxemW\|c3nvckBifCBzIIXNJIlv[3WkYYTl[EBnd3JiMTDkZZkh[nlicYXhcpRqfGG2aY\lJHBEWiCvZYToc4QhemWuYYTpeoUhfG9idX70doVifGWmIHPvcpRzd2x?	NF;qZpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS5O\|E{Oid-MkW0PVcyOzJ:L3G+
HepaR	NFrVT5VHfW6ldHnvckBie3OjeR?=	MmrPNUB2VQ>?	M2f2flEh\GG7	M1LKOWFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIHj1cYFvKEincHHSJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEiPR1PTNkBo\W6nIHX4dJJme3Orb36gZZQhOSC3TTDpcoN2[mG2ZXSg[o9zKDFiZHH5JIJ6KHG3YX70bZRifGm4ZTDQR3IhdWW2aH;kJJJmdGG2aY\lJJRwKHWwdILlZZRm\CClb370do9t	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR7N{GzNkc,OjV2OUexN\|I9N2F-
C2C12	MYTGeY5kfGmxbjDhd5NigQ>?	MWqzNEB2VQ>?	M{ewT\|IhcHK\|	NY\FfVlPUW6mdXP0bY9vKG:oIEKt[IVwgHlvW{PIYU1odHWlb4PlJJVxfGGtZTDpckBud3W\|ZTDDNmMyOiClZXzsd{BifCB\|MDD1UUBi\nSncjCyJIhzeyCkeTDsbZF2cWRic3PpcpRqdGyjdHnvckBkd3WwdHnu[{BidmGueYPpdy=>	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh\|NUWzO{c,OjV6M{W1N\|c9N2F-
MCF7	NVHsR49vTnWwY4Tpc44h[XO\|YYm=		MX:xOkBpenN?	NHvF[5FC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUB1emGwc3\lZ5Rm\CCrbjDoeY1idiCPQ1[3JINmdGy\|IHHmeIVzKDF4IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlA5PyEQvF2u	NYP5NWxIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yNlY1QTBpPkK2NlI3PDlyPD;hQi=>
HEK293	Ml;mSpVv[3Srb36gZZN{[Xl?	MnK1NVAhfU1?	NUDBXYVQOjRiaILz	M{XqZmFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{Bie3Onc4Pl[EBieyC{ZXPldJRweiC2cnHud4FkfGm4YYTpc44h[XRiMUCgeW0hcW6ldXLheIVlKG[xcjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6	NFL4U\|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO4OFI5Pid-Mk[zPFQzQDZ:L3G+
3T3L1	NUjodmlTTnWwY4Tpc44h[XO\|YYm=	MW[yJJVO		MlH5WJJidnOjY4TpeoF1cW:wIH;mJHBRSVKpYX3tZUBqdiCvb4Xz[UA{XDOOMTDj[YxteyCjc4Pld5Nm\CCjczDh[Ilxd2O7dHWg[Iln\mW{ZX70bYF1cW:wIHL5JI1m[XO3cnnu[{BtcXCrZDDhZ4N2dXWuYYTpc44h[XRiMjD1UUBjgSCRaXygVoVlKE9ic4ThbY5qdmdvYnHz[YQh[XO\|YYm=	NXKxNXZrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVU4PDlpPkK2OVk2PzR7PD;hQi=>
3T3L1	M3LtdmZ2dmO2aX;uJIF{e2G7	NUjqZ5ZrOiC3TR?=		NX\WNWxuXHKjboPhZ5RqfmG2aX;uJI9nKFCSQWLnZY1u[SCrbjDtc5V{\SB\|VEPMNUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCIQVLQOEBmgHC{ZYPzbY9vKGG2IEKgeW0h[nlicWDDVkBu\XSqb3S=	NV74cIhjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVU4PDlpPkK2OVk2PzR7PD;hQi=>
3T3L1	MnXqSpVv[3Srb36gZZN{[Xl?	M4HMfFIhfU1?		M3zjTXRz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|TEGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hT1SXVESg[ZhxemW\|c3nvckBifCB{IIXNJIJ6KHGSQ2KgcYV1cG:m	M1Twc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUm1O\|Q6Lz5{NkW5OVc1QTxxYU6=
3T3L1	MVLGeY5kfGmxbjDhd5NigQ>?	MXmyJJVO		NGPaeXZVemGwc3HjeIl3[XSrb36gc4YhWFCDUnfhcY1iKGmwIH3veZNmKDOWM1yxJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGGmaYDvcoVkfGmwIHX4dJJme3Orb36gZZQhOiC3TTDifUByWEOUIH3leIhw\A>?	M1XYXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUm1O\|Q6Lz5{NkW5OVc1QTxxYU6=
3T3L1	NEjS[YVHfW6ldHnvckBie3OjeR?=	Mn\zNkB2VQ>?		M1LXU3Rz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|TEGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hVFCOIHX4dJJme3Orb36gZZQhOiC3TTDifUByWEOUIH3leIhw\A>?	NX71Oo1KRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVU4PDlpPkK2OVk2PzR7PD;hQi=>
HEK293	Mn7kSpVv[3Srb36gZZN{[Xl?	NWPX[4g3OSC3TR?=	NYnx[VRZOjRiaILz	NUfrcVA3SWexbnnzeEBi[3Srdnn0fUBifCCSUFHSJIdidW2jIDj1cotvd3ewIH;ybYdqdilidILhcpNn\WO2ZXSgbY4hUEWNMkmzJINmdGy\|IHH0JFEhfU1iYX\0[ZIhOjRiaILzJIJ6KGS3YXytcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6	M{LOU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUi4N\|A1Lz5{Nkm4PFMxPDxxYU6=
C2C12	NH64OpFHfW6ldHnvckBie3OjeR?=	M2jOWFIxKHWP	M4j4dlQhcHK\|	MkLyWJJidnOjY4TpeoF1cW:wIH;mJHBRSVKpYX3tZUBqdiCvb4Xz[UBEOkNzMjDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBIVFWWLUSgcYVlcWG2ZXSgNk1PSkSJIIXweIFs\SCjdDCyNEB2VSCjdDC0JIhzeyCkeTDmcJVwemW\|Y3XuZ4Uh[XO\|YYm=	NVvidppNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNFA6QTNpPkK3NVAxQTl\|PD;hQi=>
HEK293	M2TqeGZ2dmO2aX;uJIF{e2G7		MYSyOEBpenN?	M3m2[WFod26rc4SgZYN1cX[rdImgZZQhT0GOND30ZYdo\WRiaIXtZY4hWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDjbIlu\XKrYzDy[YNmeHSxcjDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlI3KM7:TT6=	NFLKZ249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{W5NVAxPid-Mke1PVExODZ:L3G+
HepG2	NHjhWFZHfW6ldHnvckBie3OjeR?=		NVfpW5Z1OjBiaILz	NI\PWoRVemGwc3HjeIl3[XSrb36gc4YhT0GOND3meZNm\CCqdX3hckBRWEGUZ3HtcYEhVEKGIHX4dJJme3OnZDDpckBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjCyNEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yNDFOwG0v	MkPCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyN{[4NlcoRjJ6MEe2PFI4RC:jPh?=
HT-29	NWm3[WxiTnWwY4Tpc44h[XO\|YYm=	NWPtZnZMOTBidV2=	NH[4UHEzPCCqcoO=	NUm4WXJMSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gbJVu[W5iSGStNlkh[2WubIOgbIFz[m:{aX7nJGFRSyCvdYThcpQh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBYdnRxYnX0ZU1k[XSnbnnuJJNq\26jbHnu[{Bx[XSqd3H5JIJ6KG2nYYP1dolv\yCmZXPy[YF{\SCrbjDDfYNtcW6GMTDs[ZZmdCCjdDCxNEB2VSC2cnXheIVlKG[xcjCyOEBpenNiYomgW4V{fGW{bjDicI91KG2ndHjv[C=>	NHfaRo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC3OlgzPyd-MkiwO\|Y5Ojd:L3G+
COS7	NYDndopZTnWwY4Tpc44h[XO\|YYm=		MkDlNlQhcHK\|	MXTUdoFve2GldHn2ZZRqd25ib3[gbJVu[W5iR3HsOE1nfXOnZDDQVGFT\2GvbXGgUGJFKGW6cILld5Nm\CCrbjDB[pJq[2GwIHfy[YVvKG2xbnvlfUBEV1N5IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCwOEDPxE1w	MnftQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4MEm3NFgoRjJ6NkC5O\|A5RC:jPh?=
COS7	MUfGeY5kfGmxbjDhd5NigQ>?		M3fT[lI1KGi{cx?=	MUjUdoFve2GldHn2ZZRqd25ib3[gbJVu[W5iR3HsOE1nfXOnZDDQVGFT[WyyaHGgUGJFKGW6cILld5Nm\CCrbjDB[pJq[2GwIHfy[YVvKG2xbnvlfUBEV1N5IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSBzMDFOwG0v	MlryQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4MEm3NFgoRjJ6NkC5O\|A5RC:jPh?=
HEK293	NX2z[Yc4TnWwY4Tpc44h[XO\|YYm=		MmrhNVghcHK\|	MUPB[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRvZoXz[YQhWFCDUnfhcY1iKEyERDCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVk\XC2b4KgeJJidnOjY4TpeoF1cW:wIHHmeIVzKDF6IHjyd{BjgSCmdXHsJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMESg{txONg>?	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ4N{i3Okc,Ojh4Nke4O\|Y9N2F-
Ac2F	MWPGeY5kfGmxbjDhd5NigQ>?	NWXEbnF7PSC3TR?=	MX[2JIhzew>?	M{PPcmFod26rc4SgZYN1cX[rdImgZZQh\nWubDDs[Y5ofGhiaIXtZY4h\myjZz30ZYdo\WRiUGDBVodidW2jMTDMRmQh\XiycnXzd4VlKGmwIILheEBC[zKIIHPlcIx{KGOxZYjwdoV{e2mwZzDQVHJGNVh\|LWTLJIF1KDVidV2gZYZ1\XJiNjDodpMh[nlib37lMYdtdyCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYm=	M1XSWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{i5NVIyLz5{OUO4PVEzOTxxYU6=
SK-N-MC	NELpRnpyUFSVIHHzd4F6			MVTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?=	MlrrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
3T3-L1	NV7rTmVmTnWwY4Tpc44h[XO\|YYm=	MlzTNUB2VQ>?	M4DuNlE2KGSjeYO=	M{TQNWFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIH3veZNmKDOWMz3MNUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCFREO2JI1TVkFiZYjwdoV{e2mxbjDheEAyKHWPIHHmeIVzKDF3IHThfZMh[nlicYXhcpRqfGG2aY\lJJJm[WxvdHnt[UBRS1JibXX0bI9l	MofiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl6N{i3OlcoRjJ7OEe4O\|Y4RC:jPh?=
3T3-L1	NFv6fJFHfW6ldHnvckBie3OjeR?=	MXyxJJVO	MVWxOUBl[Xm\|	MUfB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCvb4Xz[UA{XDNvTEGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKGGmaYDvZ5l1\SCmaX\m[ZJmdnSrYYTpc44h[XRiMTD1UUBi\nSncjCxOUBl[Xm\|IHL5JG9qdCCUZXSgU{B{fGGrbnnu[{1j[XOnZDDhd5NigQ>?	M3zUW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OEe4O\|Y4Lz5{OUi3PFc3PzxxYU6=
3T3-L1	M{jwWWZ2dmO2aX;uJIF{e2G7	NYfvU5lyOSC3TR?=	MlHSNVUh\GG7cx?=	NX\2[HV4SWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gcY92e2ViM2SzMWwyKGOnbHzzJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIFPQWFFCKG2UTlGg[ZhxemW\|c3nvckBifCBzIIXNJIFnfGW{IEG1JIRigXNiYomgdZVidnSrdHH0bZZmKHKnYXyteIlu\SCSQ2KgcYV1cG:m	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTh5OEe2O{c,Ojl6N{i3Olc9N2F-
HepG2	NW[2THBiTnWwY4Tpc44h[XO\|YYm=		MV:yNEBpenN?	MmPnWJJidnOjY4TpeoF1cW:wIH;mJGdCVDRvdHHn[4VlKGi3bXHuJHBRSVKpYX3tZUBNSkRiZYjwdoV{e2WmIHnuJIh2dWGwIFjldGczKGOnbHzzJIFnfGW{IEKwJIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjB\|OTFOwG0v	NFTFTpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEG5PVI2Oyd-M{CxPVkzPTN:L3G+
3T3L1	MojTSpVv[3Srb36gZZN{[Xl?	MV:1JJVO		NWW0[4I5UW6mdXP0bY9vKG:oIHzpdIllKGGlY4XteYxifGmxbjDpckBud3W\|ZTCzWFNNOSClZXzsd{BifCB3IIXNJIJ6KE:rbDDS[YQhVyC\|dHHpcolv\y2kYYPl[EBie3OjeR?=	NEnrO2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEG5PVI2Oyd-M{CxPVkzPTN:L3G+
3T3L1	NVzTd5BGTnWwY4Tpc44h[XO\|YYm=	NGPBd4szKHWP		MlrFTY5lfWO2aX;uJI9nKGyrcHnkJIFk[3WvdXzheIlwdiCrbjDtc5V{\SB\|VEPMNUBk\WyuczDheEAzKHWPIHL5JG9qdCCUZXSgU{B{fGGrbnnu[{1j[XOnZDDhd5NigQ>?	NV3tW3RORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CxPVkzPTNpPkOwNVk6OjV\|PD;hQi=>
HepG2	NF\Hd5dHfW6ldHnvckBie3OjeR?=	M13jSV4yODBibl2=	MlHHNlAhcHK\|	NFHu[m1VemGwc3HjeIl3[XSrb36gc4YhT0GOND30ZYdo\WRiaIXtZY4hWFCDUnfhcY1iKEyERDDlfJBz\XO\|ZXSgbY4hcHWvYX6gTIVxTzJiY3XscJMh[XRiPkGwNEBvVSCjZoTldkAzOCCqcoOgbY4heHKnc3XuZ4Uhd2ZiNTD1UUBRWEGUZ3HtcYEh[W62YXfvcol{fCCJV{m2OlIh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5	NIOycWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEG5PVI2Oyd-M{CxPVkzPTN:L3G+
COS7	NYjNXnJCTnWwY4Tpc44h[XO\|YYm=		M4\yOVI1KGi{cx?=	MWLB[49vcXO2IHHjeIl3cXS7IHH0JJJm[2:vYnnuZY51KGi3bXHuJGdCVDRvRFLEJIZ2e2WmIGDQRXJo[W2vYTDMRmQh\XiycnXzd4VlKGmwIFPPV\|ch[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvOzl4IN88UU4>	MlLxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{MkC2NFIoRjNyMkKwOlAzRC:jPh?=
COS7	NV;mUYJJTnWwY4Tpc44h[XO\|YYm=		M4PtZVI1KGi{cx?=	MWLB[49vcXO2IHHjeIl3cXS7IHH0JJJm[2:vYnnuZY51KGi3bXHuJGdCVDRvRFLEJIZ2e2WmIGDQRXJo[W2vYTDMRmQh\XiycnXzd4VlKGmwIFPPV\|ch[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvOzl4IN88UU4>	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ2MUmwO{c,OzB{NEG5NFc9N2F-
HEK293	M2PBPGZ2dmO2aX;uJIF{e2G7		MUOxPEBpenN?	MXHB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFfBUFQh\nW\|ZXSgVHBCWmejbX3hMWxDTCCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGGodHXyJFE5KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkGyJO69VS5?	NX7rVWFSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyPVY3QDVpPkOwNlk3Pjh3PD;hQi=>
HEL 293H	NWDDNZVkTnWwY4Tpc44h[XO\|YYm=		MUGxOkBpenN?	NGW0XJZVemGwc3HjeIl3[XSrb36gc4YhT0GOND3ERmQh\nW\|ZXSgbJVu[W5iUGDBVodidW2jIHzp[4Fv\CCkaX7kbY5oKGSxbXHpckBmgHC{ZYPz[YQhcW5iVVHTMYJt[SCKRVygNlk{UCClZXzsd{BxemWrbnP1ZoF1\WRiZn;yJFE3KGi{czDmc4xtd3enZDDifUBHWkWWIIP1ZpN1emG2ZTDh[IRqfGmxbjDhcoQhdWWjc4Xy[YQh[W[2ZYKgNkBpenNiYomgWHIuTlKHVDDhd5NigSxiRVO1NEA:KDBwMEC4JO69VS5?	M1LJcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyNEK5NFk4Lz5\|MESyPVA6PzxxYU6=
HEK293	MUDGeY5kfGmxbjDhd5NigQ>?		NHy4fHUyQCCqcoO=	Mo\1WJJidnOjY4TpeoF1cW:wIH;mJIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHnuZ5Vj[XSnZDDmc5IhOThiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yM{mg{txONg>?	M4P6TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=
CV-1	NHvZellHfW6ldHnvckBie3OjeR?=			MofDWIV{fGWmIH\vdkB1emGwc3HjeIl3[XSrb36gc4YhcHWvYX6gVHBCWiCpYX3tZU1I[Wx2IHPobY1memFiaX6gR3YuOSClZXzsd{whTUN3MDC9JFAvODVizszNMi=>	M1TkWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=
3T3L1	M1O3NmZ2dmO2aX;uJIF{e2G7		NW\mXFdjPyCmYYnz	M2nYVnVxemWpdXzheIlwdiCxZjDGRXMheHKxdHXpckBmgHC{ZYPzbY9vKGmwIHTp[oZmemWwdHnheIVlKG2xdYPlJFNVO0xzIHPlcIx{KGGodHXyJFch\GG7czDifUB4\XO2ZYLuJIJtd3RiYX7hcJl{cXN?	M3nnS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=
3T3L1	M3;xUWZ2dmO2aX;uJIF{e2G7		MnLMO{Bl[Xm\|	MX\VdJJm\3WuYYTpc44hd2ZiYWCyJJBzd3SnaX6g[ZhxemW\|c3nvckBqdiCmaX\m[ZJmdnSrYYTl[EBud3W\|ZTCzWFNNOSClZXzsd{Bi\nSncjC3JIRigXNiYomge4V{fGW{bjDicI91KGGwYXz5d4l{	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlEwLz6FaFXNRmw9N2F-
3T3L1	MWDGeY5kfGmxbjDhd5NigQ>?		NVjJXHNJOjRiaILz	NV\1TGppUW6qaXLpeIlwdiCxZjDjbJJwdmmlIHnud5VtcW5vc4TpcZVt[XSnZDDpcpN2dGmwIILld4l{fGGwY3WgbY4h\GmoZnXy[Y51cWG2ZXSgcY92e2ViM2SzUFEh[2WubIOgZZN{\XO\|ZXSgZZMhOi2mZX;4fU1FNVt\|SG2t[4x2[2:\|ZTD1dJRic2ViYX\0[ZIhOjRiaILzJIJ6KHOlaX70bYxt[XSrb36gZ492dnSrbnegZY5idHm\|aYO=	M1zMcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=
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3T3L1	Mlq0SpVv[3Srb36gZZN{[Xl?	M2T5ZVIhfU1?	M2\QO\|E1KGSjeYO=	M2DaNGFod26rc4SgZYN1cX[rdImgZZQhWFCDUjDpckBud3W\|ZTCzWFNNOSClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDh[Ilxd2O7dHWg[Iln\mW{ZX70bYF1cW:wIHH0JFIhfU1iYX\0[ZIhOTRiZHH5d{BjgSCRaXygVoVlKE9ic4ThbY5qdmdibXX0bI9l	M4PGbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=
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Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	Cyclin D1 / CDK2 / CDK4 / Bax / Bcl-2	29725405
Immunofluorescence	PPARγ / E-cadherin	27145370
Growth inhibition assay	Cell viability	29725405

In vivo

Rosiglitazone administration results in significant bone loss, including a decrease in bone volume, trabecular width, and trabecular number and an increase in trabecular spacing. Rosiglitazone also leads to a decrease in bone formation rate, with a simultaneous increase in fat content in the bone marrow. Rosiglitazone decreases the expression of the osteoblast-specific genes Runx2/Cbfa1, Dlx5, and alpha1(I)collagen, whereas the expression of the adipocyte-specific fatty acid binding protein aP2 is increased. ^[2] Rosiglitazone upregulates gene transcripts encoding mitochondrial proteins in white adipocytes from ob/ob mice accompanied by an increase in mitochondrial mass and changes in mitochondrial structure. ^[4]

Protocol (from reference)

References

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Solubility (25°C)

In vitro


In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): 4% DMSO+30% PEG 300+5% Tween 80+ddH2O For best results, use promptly after mixing. Click to purchase: PEG300 Tween 80	5mg/mL

Chemical Information

Molecular Weight	357.43
Formula	C₁₈H₁₉N₃O₃S
CAS No.	122320-73-4
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CN(CCOC1=CC=C(C=C1)CC2C(=O)NC(=O)S2)C3=CC=CC=N3

Download Rosiglitazone (BRL 49653) SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

Clinical Trial Information

NCT Number	Recruitment	Interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT02737709	Terminated	Drug: Paclitaxel and Carboplatin regimen	Non-small Cell Lung Cancer	Sun Yat-sen University	March 2016	Phase 2
NCT01402076	Completed	Drug: Tasimelteon\|Drug: Rosiglitazone\|Drug: Midazolam	Healthy Volunteers	Vanda Pharmaceuticals	August 2011	Phase 1
NCT01100619	Completed	Drug: rosiglitazone\|Drug: XL184	Papillary Thyroid Cancer\|Follicular Thyroid Cancer\|Huerthle Cell Thyroid Cancer\|Renal Cell Carcinoma	Exelixis	April 2010	Phase 1
NCT01415128	Completed	Drug: Omeprazole\|Drug: Rosiglitazone\|Drug: Desipramine	Erectile Dysfunction	VIVUS LLC	April 2010	Phase 1
NCT01376063	Completed	Drug: FG-4592	Healthy Adult Subjects	FibroGen	March 2010	Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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