Home Metabolism PPAR agonist - TRP Channel modulator - Autophagy activator - Ferroptosis inhibitor Rosiglitazone (BRL 49653)

Rosiglitazone (BRL 49653)

Catalog No.S2556

For research use only.

Rosiglitazone (BRL 49653) is a potent antihyperglycemic agent and a potent thiazolidinedione insulin sensitizer with IC50 of 12, 4 and 9 nM for rat, 3T3-L1 and human adipocytes, respectively. Rosiglitazone is a pure ligand of PPAR-gamma, and has no PPAR-alpha-binding action. Rosiglitazone modulates TRP channels and induces autophagy. Rosiglitazone prevents ferroptosis.

Rosiglitazone (BRL 49653) Chemical Structure

CAS No. 122320-73-4

Selleck's Rosiglitazone (BRL 49653) has been cited by 49 publications

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Biological Activity

Description Rosiglitazone (BRL 49653) is a potent antihyperglycemic agent and a potent thiazolidinedione insulin sensitizer with IC50 of 12, 4 and 9 nM for rat, 3T3-L1 and human adipocytes, respectively. Rosiglitazone is a pure ligand of PPAR-gamma, and has no PPAR-alpha-binding action. Rosiglitazone modulates TRP channels and induces autophagy. Rosiglitazone prevents ferroptosis.
Targets
PPAR [1]
(Cell-free assay)		 TRPC [5] Ferroptosis [6]
In vitro

Rosiglitazone markedly increase phosphorylation of threonine 172 within the α subunit of AMPK, with increased AMP:ATP ratio. Rosiglitazone has been reported to decrease cholesterol synthesis in a number of cell lines in a peroxisome proliferator-activated receptor γ-independent manner. Rosiglitazone activates both α1- and α2-containing AMPK complexes, and this leads to a marked increase in the phosphorylation of acetyl-CoA carboxylase. [1] Rosiglitazone activates PPAR-gamma2 which acts as a dominant inhibitor of osteoblastogenesis in murine bone marrow in vitro.[2] Rosiglitazone increases adiponectin secretion from omental cells up to 2.3-fold higher, whereas secretion from subcutaneous adipose cells is unaffected. [3] Rosiglitazone changes the morphological features and protein profiles of mitochondria in 3T3-L1 adipocytes. [4]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 MXPGeY5kfGmxbjDhd5NigQ>? MVPF[oZm[3SrdnWgZ49v[2WwdILheIlwdiCjZ3HpcpN1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSHXwS|Ih[2WubIOsJGVEPTB;MD6wNkDPxE1? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPThyMUixO{c,OTV6MEG4NVc9N2F-
CV-1 MlrISpVv[3Srb36gZZN{[Xl? M2LDVWlvKH[rdILvJJRz[W6|Y4LpdJRqd26jbDDhZ5RqfmG2aX;uJI9nKFCncn;4bZNwdWVicILvcIln\XKjdH;yJIFkfGm4YYTl[EBz\WOncITvdkBo[W2vYTCoVHBCWiliZYjwdoV{e2WmIHnuJGNXNTFiY3XscJMtKEWFNUCgQUAxNjB4IN88UU4> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85PTd4OUC3K|45PTd4OUC3QE9iRg>?
CV-1 NGLmVFVHfW6ldHnvckBie3OjeR?= MU\BZ5RqfmG2aX;uJI9nKHCncn;4bZNwdWVicILvcIln\XKjdH;yJIFkfGm4YYTl[EBz\WOncITvdkBo[W2vYTDt[YF{fXKnZDDifUBqdmS3Y4Tpc44hd2ZiNUClJI9nKG2jeHnteY0h[WytYXzpcoUheGixc4DoZZRie2ViYXP0bZZqfHluIITyZY5{\mWldHnvckBie3OjeTDpckBEXi1zIHPlcIx{NCCHQ{WwJF0hOC5yOEmxN{DPxE1w MnXQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTh|Nk[yNEc,QTh|Nk[yNFww[T5?
3T3-L1 MXPGeY5kfGmxbjDhd5NigQ>? MX\TeIlufWyjdHnvckBw\iCjZHnwc4dmdmW|aYOgbY4hO1R|LVyxJINmdGy|IHnzJIV5eHKnc4Pl[EBieyClb37j[Y51emG2aX;uJIVyfWm4YXzlcpQhfG9idHjlJHshOS1zNFPdJJVxfGGtZTDjc5VvfHNiYX\0[ZIhfHKnYYTt[Y51KHerdHigNE4zKHWpL33MJJRzd2euaYThfo9v\SxiRVOgQUAxNjB7IN88UU4> M1\ZRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyOUW2NlE{Lz5zMEm1OlIyOzxxYU6=
CV-1 MUTGeY5kfGmxbjDhd5NigQ>? M1\tcW1igGmvYXygdoVxd3K2ZYKgZYN1cX[rdImgZYdicW6|dDDoeY1idiCSZYLvfIl{d22nIIDyc4xq\mW{YYTvdkBi[3SrdnH0[YQhemWlZYD0c5Ih\2GvbXGgS4FtPCClaHnt[ZJq[yCrbjD0doFve2mnboTsfUB1emGwc3\lZ5Rm\CCFVj2xJINmdGy|IHL5JIZ2dmO2aX;uZYwh[XO|YYmuMEBGSzVyIE2gNE4xPDNizszNMi=> MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTd{MEi1OEc,OTF5MkC4OVQ9N2F-
COS1 NELYcWlHfW6ldHnvckBie3OjeR?= NFHBVYpC\2:waYP0bYMh[WO2aY\peJkhf2G|IHTleIVzdWmwZXSgbY4hS0:VMTDj[YxteyC2cnHud4Zm[3SnZDD3bZRpKEeDTDC0MXBRSVJiZ3HtcYEhemWlZYD0c5ItKEWFNUCgQUAxNjB{MTFOwG0v MmPZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ4MUe5NlQoRjF{NkG3PVI1RC:jPh?=
COS1 MXnGeY5kfGmxbjDhd5NigQ>? Mne5TY4hfmm2cn:gZYdwdmm|dDDhZ5Rqfmm2eTD0[ZN1\WRiZn;yJJRz[W6|YXP0bZZifGmxbjDpckBpfW2jbjDQVGFTKGejbX3hMWdidDRiY3jpcYVzcWNiQ1;TMVEh[2WubIOsJGVEPTBiPTCwMlAzKM7:TT6= MlHiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ5Mkm2OlgoRjF{N{K5OlY5RC:jPh?=
COS1 MV\GeY5kfGmxbjDhd5NigQ>? NW\SUVduUW5idnn0do8hfHKjboPhZ5RqfmG2aX;uJI9nKGi3bXHuJJBmem:6aYPvcYUheHKxbHnm[ZJifG:{IHHjeIl3[XSnZDDy[YNmeHSxcjDnZY1u[SCvZXHzeZJm\CCrbjDQVGFTNUeDTESgZ4hqdWW{aXOgR29UNTFiY3XscJMtKEWFNUCgQUAxNjB{IN88UU4> NV6wS4tiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK4O|M2OTdpPkGyPFc{PTF5PD;hQi=>
CV-1 NEXYNXlHfW6ldHnvckBie3OjeR?= MkexTY4hfmm2cn:g[ZZidHWjdHnvckBi\2GrboP0JHJZWi2jbIDoZU9RWEGULXfhcY1iKGmwIFPWMVEh[2WubIOgZpkh[2:2cnHud4Zm[3Srb36gZZN{[Xlid3HzJIRmfGW{bXnu[YQtKEWFNUCgQUAxNjN{NTFOwG0v MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjl3NEC2NUc,OTJ7NUSwOlE9N2F-
3T3-L1 MmHnSpVv[3Srb36gZZN{[Xl? M{DPcGVn\mWldHn2[UBkd26lZX70doF1cW:wIH\vdkBmdmijbnPlcYVvfCCxZjDpcpN2dGmwLXnu[JVk\WRidILp[4x6[2W{aXTlJIFk[3WvdXzheIlwdiCrbjCzWFMuVDFiY3XscJMtKEWFM{CgQUAxNjB{IN88UU4> NXTMRWt4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS1NFU3PDRpPkG0OVA2PjR2PD;hQi=>
HepG2 MU\GeY5kfGmxbjDhd5NigQ>? NGHpSpdG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDh[4FqdnO2IHj1cYFvKFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTIVxTzJiY3XscJMtKEWFNUCgQUAxNjB|OTFOwG0v MlTyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZzMEexOVAoRjF4MUC3NVUxRC:jPh?=
U2OS M4HHVGZ2dmO2aX;uJIF{e2G7 MYjF[oZm[3Rib36gVHBCWmejbX3hJJRz[W6|YXP0bZZifGmxbjDhZ5Rqfmm2eTDpckBWOk:VIHPlcIx{NCCHQ{WwJF0hOC5yMzFOwG0v Moi1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ|MEC5OFQoRjF4M{CwPVQ1RC:jPh?=
Huh7 NX:0TlllTnWwY4Tpc44h[XO|YYm= MmPnSpVv[3Srb37hcEBi[3Srdnn0fUBifCCqdX3hckBRWEGUIHfhcY1iKGmwIFj1bFch[2WubIOgZpkhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6yNkDPxE1w MmDqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ|Nk[2NFEoRjF4M{[2OlAyRC:jPh?=
Huh7 M4T6RmZ2dmO2aX;uJIF{e2G7 M3;vW2Vn\mWldDDvckBRWEGUIHfhcY1iKHS{YX7zZYN1cX[jdHnvckBi[3Srdnn0fUBqdiCKdXi3JINmdGy|LDDFR|UxKD1iMD6yNkDPxE1w MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjR3MUC4O{c,OTZ2NUGwPFc9N2F-
HEK293 M33lV2Z2dmO2aX;uJIF{e2G7 MleyRYdwdmm|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gdoVk\XC2b4KgbY51\XKjY4Tpc44hf2m2aDDzeIVzd2mmIILlZ4VxfG:{IHPvZYN1cX[jdH;yMVEh[nliRWnGVEBj[XOnZDDy[ZBwenSncjDn[Y5mKGG|c3H5 M4LyVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
HEK293 MnLlSpVv[3Srb36gZZN{[Xl? MmO4RYdwdmm|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gdoVk\XC2b4KgbY51\XKjY4Tpc44hf2m2aDDy[ZRqdm:rZDDYMZJm[2WydH;yJIFteGijIHL5JGV[TlBiYnHz[YQhemWyb4L0[ZIh\2WwZTDhd5NigQ>? M1zQ[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
CV1 NXzCUFhYTnWwY4Tpc44h[XO|YYm= MV3UdoFve2GldHn2ZZRqd25ib3[gVHBCWmejbX3hJIlvKEOYMTDj[YxteyxiRVO1NEA:KDBwMEe2JO69VS5? M3X6bVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OEKxO|Y6Lz5zNkiyNVc3QTxxYU6=
NIH3T3 M3\XSGZ2dmO2aX;uJIF{e2G7 NE\1VmVC[3Srdnn0fUBifCCqdX3hckBRWEGUIHfhcY1iKHS{YX7z[oVkfGWmIHnuJG5KUDOWMzDj[YxteyCkeTDseYNq\mW{YYPlJIFkfGm4aYT5JIF{e2G7LDDFR|UxKD1iMD6zNkDPxE1w NVvDR5JJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[4OVQxQDVpPkG2PFU1ODh3PD;hQi=>
CV1 MWXGeY5kfGmxbjDhd5NigQ>? MUnUdoFve2GldHn2ZZRqd25ib3[gbJVu[W5iUGDBVodidW2jIHnuJGNXOSClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTBiPTCwMlMxQCEQvF2u NUPnNmtVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5O|A{QTFpPkG2PVcxOzlzPD;hQi=>
HepG2 MVPGeY5kfGmxbjDhd5NigQ>? M3nlcWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWiCpYX3tZUBqdiCjIFjldGczKGOnbHzzJIJ6KFCSQWKtS2FNPCC2cnHud4FkfGm4YYTpc44h[XO|YYmsJGVEPTBiPTCwMlE2QCEQvF2u M4PuS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUezN|U5Lz5zNkm3N|M2QDxxYU6=
HepG2 MYrGeY5kfGmxbjDhd5NigQ>? MUXB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXIh\2GvbXGgbY4hUGWyR{KgZ4VtdHNiYomgVHBCWi2JQVy0JJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMUW4JO69VS5? MnvPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7N{mzOFEoRjF4OUe5N|QyRC:jPh?=
CV1 MYjGeY5kfGmxbjDhd5NigQ>? NYDiOmFSSWO2aY\peJkh[XRiaIXtZY4hWFCDUnfhcY1iKGmwIFPWNUBk\WyuczygSWM2OCB;IECuN|A5KM7:TT6= M1LHOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MEC1N|k1Lz5zN{CwOVM6PDxxYU6=
Huh7 MU\GeY5kfGmxbjDhd5NigQ>? NFHZ[FVC[3Srdnn0fUBifCCqdX3hckBRWEGUIHfhcY1iKGmwIFj1bFch[2WubIOgZpkhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6yNkDPxE1w MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzB|NEG0PUc,OTdyM{SxOFk9N2F-
CV1 NIH1XndHfW6ldHnvckBie3OjeR?= NWnU[oprSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gR3YyKGOnbHzzJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuN|A5KM7:TT6= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF{OUeyOUc,OTdzMkm3NlU9N2F-
HepG2 NULhdm5oTnWwY4Tpc44h[XO|YYm= NX\rcJdMSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEhcW5iSHXwS|Ih[2WubIOgZpkhWFCDUj3HRWw1KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNFgzKM7:TT6= NEDCNFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G1O|AyQSd-MUexOVcxOTl:L3G+
HEK293 MkPBSpVv[3Srb36gZZN{[Xl? NHPEeY5C\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SC{ZXPldJRweiCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGK7IFfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6wN|Uh|ryPLh?= MkXyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd{OUK2NFYoRjF5MkmyOlA3RC:jPh?=
HEK293 M1OwOmZ2dmO2aX;uJIF{e2G7 M4XTOGFkfGm4aYT5JIF1KGi3bXHuJIFlcXCxc3WgeIl{e3WnIGDQRXIh\2GvbXGg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDifUBRWEGULVfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6zNUDPxE1w MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN2M{O3NUc,OTd|NEOzO|E9N2F-
L6 NVrBcXN1TnWwY4Tpc44h[XO|YYm= M1;JcGVn\mWldDDvckBn[XS2eTDhZ4llKG:6aXTheIlwdiCrbjDyZZQhVDZiY3XscJMtKEWFNUCgQUA2KM7:TT6= M{\ZeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{SzN|cyLz5zN{O0N|M4OTxxYU6=
HepG2/C3A MlzBSpVv[3Srb36gZZN{[Xl? NU\OOVlMSWO2aY\heIlwdiCxZjDoeY1idiCSUFHS[4FudWFibHnnZY5lKGKrbnTpcoch\G:vYXnuMY1m\GmjdHXkJJRz[W6|Y4LpdJRqd26jbDDhZ5Rqfmm2eTDpckBpfW2jbjDI[ZBIOi:FM1GgZ4VtdHNiY3:teJJidnOoZXP0[YQhf2m2aDDmeZNm\CCJYXy0MWxDTCCkeTDjc5Rz[W6|ZnXjeIlwdiCjc4PhfUwhTUN3MDC9JFAvODB|ODFOwG0v MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRyM{[4PEc,OTd2MEO2PFg9N2F-
CV1 NVfmU3diTnWwY4Tpc44h[XO|YYm= NILOb2QzPCCqcoO= MnPLRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgbY4hS1ZzIHPlcIx{KGK7IFfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7IHHmeIVzKDJ2IHjyd{whTUN3MDC9JFAvODNizszNMi=> MnfjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MEeyNlUoRjF5NUC3NlI2RC:jPh?=
CV1 NWiyWXVtTnWwY4Tpc44h[XO|YYm= MnTQNlQhcHK| M3n4fWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmGucHjhJIlvKEOYMTDj[YxteyCkeTDHRWw1KHS{YX7zZYN1cX[jdHnvckBie3OjeTDh[pRmeiB{NDDodpMtKEWFNUCgQUA{NjR4IN88UU4> M1TtbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUC3NlI2Lz5zN{WwO|IzPTxxYU6=
NIH3T3 NYj4fGpMTnWwY4Tpc44h[XO|YYm= M{jaRmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCQSVizWFMh[2WubIOgZpkhT0GONDD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjN{IN88UU4> NID6fYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[yOFc4Pyd-MUe2NlQ4Pzd:L3G+
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CV1 MnTkSpVv[3Srb36gZZN{[Xl? M4fpZmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCFVkGgZ4VtdHNiYomgdoVk\XC2b4KgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5|MEig{txONg>? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB{OUG3PEc,OThyMkmxO|g9N2F-
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HepG2 NFW5W3BHfW6ldHnvckBie3OjeR?= NHPzS4dC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSHXwS|Ih[2WubIOgZpkhT0GONDD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjJ{MzFOwG0v MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN7NEmwO{c,OTh|OUS5NFc9N2F-
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CV1 M2focWZ2dmO2aX;uJIF{e2G7 NF[yT45C\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJIFnemmlYX6g[5Jm\W5ibX;ub4V6KEOYMTDj[YxteyClbz30doFve2[nY4Tl[EB4cXSqIH\1d4VlKEejbEStSGJFKGK7IITyZY5{[WO2aY\heIlwdiCjc4PhfUwhTUN3MDC9JFAvODN|IN88UU4> NVL6dINSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4NlYzODVpPkG4PFI3OjB3PD;hQi=>
CV1 NFrx[YlHfW6ldHnvckBie3OjeR?= MUXB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJidHCqYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIHHmdolk[W5iZ4Ll[Y4hdW:wa3X5JGNXOSClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKG[3c3XkJGdidDRvRFLEJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IEOuOFYh|ryPLh?= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDh{NkKwOUc,OTh6Mk[yNFU9N2F-
HepG2 NFPWWlhHfW6ldHnvckBie3OjeR?= MoDMRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT[WyyaHGgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDoeY1idiCKZYDHNkBk\WyuczDjc{11emGwc3\lZ5Rm\CC5aYToJGdidDRiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yM{mg{txONg>? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDh|NUexPUc,OTh6M{W3NVk9N2F-
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BHK21 MkjvSpVv[3Srb36gZZN{[Xl? NH[4W|VC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iQljLNlEh[2WubIOgZZN{\XO|ZXSgZZMhW0WDUDDhZ5Rqfmm2eTDifUBtfWOrZnXyZZNmKHKncH;yeIVzKHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuOFUh|ryPLh?= M2XjTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{S5NVc3Lz5zOUO0PVE4PjxxYU6=
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HepG2 M4\XXmZ2dmO2aX;uJIF{e2G7 NF\qPGdVemGwc3HjeIl3[XSrb36gc4YhcHWvYX6gVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\XCJMjDj[YxteyClbz30doFve2[nY4Tl[EB4cXSqIGDQVmU{NVSNLVz1Z{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTBiPTCwMlA2KM7:TT6= NHzNNmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS1NFQ3QCd-MkG0OVA1Pjh:L3G+
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Sf21 NEfrWFdHfW6ldHnvckBie3OjeR?= MkLwTY5pcWKrdHnvckBw\iCqdX3hckBDW0WSIHX4dJJme3OnZDDpckBxdGG|bXGgcYVu[nKjbnWgeoV{cWOuZYOgc4YhW2Z{MTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEGWUD3k[ZBmdmSnboSgX|NJZXSjdYLvZ4hwdGG2ZTD1dJRic2VuIFnDOVAhRSB4LkSg{txONg>? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl4NU[yN{c,OjF7NkW2NlM9N2F-
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HepG2 NUDjcI1iTnWwY4Tpc44h[XO|YYm= MV[yNEBpenN? MkLoWJJidnOjY4TpeoF1cW:wIH;mJGdCVDRvZoXz[YQhcHWvYX6gVHBCWmejbX3hJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gTIVxTzJiY3XscJMh[W[2ZYKgNlAhcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMEO5JO69VS5? M4DNRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MUexNFQ2Lz5{M{G3NVA1PTxxYU6=
L02 NVXyR4ZETnWwY4Tpc44h[XO|YYm= M3zxclI1KGi{cx?= M1njSmFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iNUyERDDlfJBz\XO|ZXSgbY4hcHWvYX6gUFAzKGOnbHzzJINwNWW6cILld5NqdmdicFfMN{1UXjRyLVfBUFQh[W[2ZYKgNlQhcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDiZZNm\CC2cnHud4FkfGm4YYTpc44h[XO|YYmsJGVEPTBiPTCwMlkh|ryPLh?= MoraQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzOE[zNFcoRjJ|MUi2N|A4RC:jPh?=
HEK293 NFTwPIxHfW6ldHnvckBie3OjeR?= NIrFZ|U1QCCqcoO= MnmwRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDjc5Rz[W6|ZnXjeIVlKHerdHigVHBTTXh2LWTLMYx2[yCjc4Pld5Nm\CCjczDhZ5RqfmG2aX;uJI9nKGy3Y3nm[ZJie2ViYXP0bZZqfHlibXXhd5Vz\WRiYX\0[ZIhPDhiaILzJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IESzMlcyKM7:TT6= NGHrUmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K2OVg1PCd-MkOyOlU5PDR:L3G+
HEK293 M2XMOWZ2dmO2aX;uJIF{e2G7 MYCxNEB2VQ>? M1uzVXRz[W6|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJINwNXS{YX7z[oVkfGWmIIfpeIghSVB{LWDQVmUh[XRiMUCgeW0h[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5 NWrjOHluRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyO|M2OTlpPkKzNlc{PTF7PD;hQi=>
HEK293 NUi0fpcyTnWwY4Tpc44h[XO|YYm= NUXK[5p7SWexbnnzeEBifCCqdX3hckBRWEGUZ3HtcYEhVEKGIHX4dJJme3OnZDDpckBpfW2jbjDISWszQTNiY3XscJMh[2:2cnHud4Zm[3SnZDD3bZRpKEeDTEStUJVkKGG|c3Xzd4VlKGG|IITyZY5{[3KrcITpc45idCCjY4TpeoF1cW:wIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMEC0JO69VS5? NYXhdVZIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyPFY4QDdpPkKzNlg3Pzh5PD;hQi=>
HEK293 MnjLSpVv[3Srb36gZZN{[Xl? Mn7rNlQhcHK| MoTFWJJidnOjY4TpeoF1cW:wIH;mJGdCVDRiRFLEMYZ2e2WmIHj1cYFvKFCSQWLnZY1u[S2OQlSg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4yKM7:TT6= MojLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OUS4N|AoRjJ|Mkm0PFMxRC:jPh?=
CMT93 MVnGeY5kfGmxbjDhd5NigQ>? NGjueI8yOCC3TR?= M4HORVMxKG2rboO= MUDJcoR2[3Srb36gc4YhWFCDUj3nZY1u[SCycn;tc5RmeiCjY4Tpeol1gSCrbjDMVHMue3SrbYXsZZRm\CCvb4Xz[UBEVVR7MzDj[YxteyCjdDCxNEB2VSCycnXpcoN2[mG2ZXSg[o9zKDNyIH3pcpMh[mWob4LlJGxRWy2laHHscIVv\2VibXXhd5Vz\WRiYX\0[ZIhPiCqcoOgdI9{fCClaHHscIVv\2ViYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 MoT5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MU[xPVAoRjJ|NEG2NVkxRC:jPh?=
HepG2 MnPySpVv[3Srb36gZZN{[Xl? MlfCRYdwdmm|dDDhZ5Rqfmm2eTDheEBISUx2LX\1d4VlKFCSQWLnZY1u[SCPNE[zRUBufXSjboSgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO|ZXSgbY4hcHWvYX6gTIVxTzJiY3XscJMh[nlidILhcpNi[3SrdnH0bY9vKGG|c3H5MEBGSzVyIE2gNE4xODJizszNMi=> NWL3dGozRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NFIzOTJpPkKzOVAzOjF{PD;hQi=>
HepG2 NVPCbZhJTnWwY4Tpc44h[XO|YYm= M3[zW2Fod26rc4SgZYN1cX[rdImgZZQhT0GOND3meZNm\CCSUFHS[4FudWFiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gbJVu[W5iSHXwS|Ih[2WubIOgZpkhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6wNUDPxE1w MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzVyMkKxNkc,OjN3MEKyNVI9N2F-
L6 MY\GeY5kfGmxbjDhd5NigQ>? MkfhNlQhcHK| NWfGXFFlUW6mdXP0bY9vKG:oIHfseYNwe2VidYD0ZYtmKGmwIILheEBNPiClZXzsd{BxfWy|ZXSge4l1cCCFMUSt[IVwgHliZ3z1Z49{\SCjZoTldkAzPCCqcoOgbY4heHKnc3XuZ4Uhd2ZiaX7zeYxqdixiRVO1NEA:KDRwNEmg{txONg>? Mnq4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7OUK4OlIoRjJ|OUmyPFYzRC:jPh?=
L6 NV\0RVdvTnWwY4Tpc44h[XO|YYm= M{K5blE3KGi{cx?= MV\JcoNz\WG|ZTDpckAzNVt|SG2t[IVwgHmpbIXjc5NmKHWydHHr[UBqdiC{YYSgUFYh[2WubIOgZYZ1\XJiMU[gbJJ{KGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoch[W6jbInzbZMtKEmFNUCgQUA1NjhizszNMi=> MkHLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUO3N|goRjJ2OEGzO|M5RC:jPh?=
HEK293 NEDadG5HfW6ldHnvckBie3OjeR?= NInQU40yOCC3TR?= NEjCfFAzPCCqcoO= MnvDWJJidnOjY4TpeoF1cW:wIH;mJHBRSVJvZ3HtcYEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF1KDFyIIXNJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk> NHLmU249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi5NFA6OCd-MkS4PVAxQTB:L3G+
HEK293 MlPmSpVv[3Srb36gZZN{[Xl? NHvsWVYzPCCqcoO= M2rtbWFod26rc4SgZYN1cX[rdImgZZQhdW:3c3WgVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZ48u\XiycnXzd4lv\yC5aYToJGdidDRicnXwc5J1\XJidnXjeI9zKGGodHXyJFI1KGi{czDifUBlfWGuLXz1Z4ln\XKjc3WgdoVxd3K2ZYKgZZN{[XluIFXDOVAhRSByLkCzJO69VS5? NYTSOnIzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OVU5QDlpPkK0PVU2QDh7PD;hQi=>
DU145 MVHDfZRwfG:6aXPpeJkh[XO|YYm= NXT2R3diPDhiaILz MnHGR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gSHUyPDViY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDF4IN88UU4> M2G1S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUm2NVQ{Lz5{NEm5OlE1OzxxYU6=
PC3 Mo\vR5l1d3SxeHnjbZR6KGG|c3H5 MXG0PEBpenN? NWnDUIpMS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWEN|IHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB{MD6zJO69VS5? NH\JfGE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm5OlE1Oyd-MkS5PVYyPDN:L3G+
MDA-MB-231 NEPXNmtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MljENlQhcHK| M4W4OWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEWuNlMh|ryPLh?= MnLrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{N{iyN|YoRjJ3Mke4NlM3RC:jPh?=
HEK293 MnLaSpVv[3Srb36gZZN{[Xl? NV\5eXhkSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTBiPTCwMlAyKM7:TT6= M1nMfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C1Olg5Lz5{NUOwOVY5QDxxYU6=
HepG2 M2TwO2Z2dmO2aX;uJIF{e2G7 Ml;lNlAhcHK| M{\XZXRz[W6|YXP0bZZifGmxbjDv[kBRWEGUIITyZY5{\mWldHXkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFIxKGi{czDifUBRWEGULXz1Z4ln\XKjc3WgZZN{[XluIFXDOVAhRSBzLk[g{txONg>? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR|N{OwOEc,OjV2M{ezNFQ9N2F-
HEK293 MnfESpVv[3Srb36gZZN{[Xl? NVzGSHlmOjRiaILz M1XBSnRz[W6|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR2MkOyNkc,OjV2NEKzNlI9N2F-
HepG2 MYTGeY5kfGmxbjDhd5NigQ>? NHPlTm1C\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGdCVDRvUGDBVodidW2jIHzp[4Fv\CCkaX7kbY5oKGSxbXHpckBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yM{mg{txONg>? MljKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NkKyPFEoRjJ3NE[yNlgyRC:jPh?=
HepG2 NWT0eJRVTnWwY4Tpc44h[XO|YYm= NWDMenhZOjBiaILz MYnB[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRvRF7BJIJqdmSrbneg[I9u[WmwIH\1d4VlKGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJIh2dWGwIFjldGczKGOnbHzzJIF{e2W|c3XkJIF{KHKnY3XweI9zKHS{YX7zZYN1cX[jdHnvckBqdmO3YnH0[YQh\m:{IEKwJIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjB2IN88UU4> MmfHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUexN|IoRjJ3NEm3NVMzRC:jPh?=
HepaR M2nKOGZ2dmO2aX;uJIF{e2G7 MV[xJJVO MXuxJIRigQ>? NVn0dVBiSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gbJVu[W5iSHXwZXIh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iRlHCVFEh\2WwZTDlfJBz\XO|aX;uJIF1KDFidV2gbY5kfWKjdHXkJIZweiBzIHThfUBjgSCzdXHueIl1[XSrdnWgVGNTKG2ndHjv[EBz\WyjdHn2[UB1dyC3boTy[YF1\WRiY3;ueJJwdA>? MlXLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUexN|IoRjJ3NEm3NVMzRC:jPh?=
HepaR MYXGeY5kfGmxbjDhd5NigQ>? NHr5VJMyKHWP NIm2WIQyKGSjeR?= M4nNT2Fod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIHj1cYFvKEincHHSJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEGQR2DUUFQh\2WwZTDlfJBz\XO|aX;uJIF1KDFidV2gbY5kfWKjdHXkJIZweiBzIHThfUBjgSCzdXHueIl1[XSrdnWgVGNTKG2ndHjv[EBz\WyjdHn2[UB1dyC3boTy[YF1\WRiY3;ueJJwdA>? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR7N{GzNkc,OjV2OUexN|I9N2F-
HepaR NVrmbo1VTnWwY4Tpc44h[XO|YYm= MU[xJJVO MVGxJIRigQ>? NHXxSWdC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCrbjDoeY1idiCKZYDhVkBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCKTVfDV|Ih\2WwZTDlfJBz\XO|aX;uJIF1KDFidV2gbY5kfWKjdHXkJIZweiBzIHThfUBjgSCzdXHueIl1[XSrdnWgVGNTKG2ndHjv[EBz\WyjdHn2[UB1dyC3boTy[YF1\WRiY3;ueJJwdA>? NH31Smc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS5O|E{Oid-MkW0PVcyOzJ:L3G+
C2C12 M4XiSWZ2dmO2aX;uJIF{e2G7 NFLuZmQ{OCC3TR?= NEW5PGYzKGi{cx?= MlHuTY5lfWO2aX;uJI9nKDJvZHXvfJkuYzOKXT3ncJVkd3OnIIXweIFs\SCrbjDtc5V{\SCFMlOxNkBk\WyuczDheEA{OCC3TTDh[pRmeiB{IHjyd{BjgSCuaYH1bYQhe2OrboTpcIxifGmxbjDjc5VvfGmwZzDhcoFtgXOrcx?= M4HjUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEO1OVM4Lz5{NUizOVU{PzxxYU6=
MCF7 MYfGeY5kfGmxbjDhd5NigQ>? NEDNXXgyPiCqcoO= MoXIRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgeJJidnOoZXP0[YQhcW5iaIXtZY4hVUOINzDj[YxteyCjZoTldkAyPiCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxKD1iMD6wPFch|ryPLh?= MkSyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{Mk[0PVAoRjJ4MkK2OFkxRC:jPh?=
HEK293 NXfrdoZjTnWwY4Tpc44h[XO|YYm= MVmxNEB2VQ>? MofwNlQhcHK| MofMRYdwdmm|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W|c3XkJIF{KHKnY3XweI9zKHS{YX7zZYN1cX[jdHnvckBifCBzMDD1UUBqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk> NHT2eGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO4OFI5Pid-Mk[zPFQzQDZ:L3G+
3T3L1 NHGweXpHfW6ldHnvckBie3OjeR?= NVrMe5ZkOiC3TR?= NXTsS2lHXHKjboPhZ5RqfmG2aX;uJI9nKFCSQWLnZY1u[SCrbjDtc5V{\SB|VEPMNUBk\WyuczDhd5Nme3OnZDDhd{Bi\Gmyb3P5eIUh\GmoZnXy[Y51cWG2aX;uJIJ6KG2nYYP1dolv\yCuaYDp[EBi[2O3bYXsZZRqd25iYYSgNkB2VSCkeTDPbYwhWmWmIF:gd5RicW6rbnetZoF{\WRiYYPzZZk> NFfYPXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5OVc1QSd-Mk[1PVU4PDl:L3G+
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3T3L1 NF6y[IhHfW6ldHnvckBie3OjeR?= M33aN|ch\GG7cx?= MVHVdJJm\3WuYYTpc44hd2ZiYWCyJJBzd3SnaX6g[ZhxemW|c3nvckBqdiCmaX\m[ZJmdnSrYYTl[EBud3W|ZTCzWFNNOSClZXzsd{Bi\nSncjC3JIRigXNiYomge4V{fGW{bjDicI91KGGwYXz5d4l{ NH3CR3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjFxJ{7DbGVOSkx:L3G+
3T3L1 NYHyXlBnTnWwY4Tpc44h[XO|YYm= NEHzdYwzPCCqcoO= NYHtd3I6UW6qaXLpeIlwdiCxZjDjbJJwdmmlIHnud5VtcW5vc4TpcZVt[XSnZDDpcpN2dGmwIILld4l{fGGwY3WgbY4h\GmoZnXy[Y51cWG2ZXSgcY92e2ViM2SzUFEh[2WubIOgZZN{\XO|ZXSgZZMhOi2mZX;4fU1FNVt|SG2t[4x2[2:|ZTD1dJRic2ViYX\0[ZIhOjRiaILzJIJ6KHOlaX70bYxt[XSrb36gZ492dnSrbnegZY5idHm|aYO= NV7yWZI2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJzLze+R4hGVUKOPD;hQi=>
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3T3L1 NW\0bJVRTnWwY4Tpc44h[XO|YYm= NVm5OWNFOiC3TR?= NYLQRZROOTRiZHH5dy=> M2fpSGFod26rc4SgZYN1cX[rdImgZZQhWFCDUjDpckBud3W|ZTCzWFNNOSClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDh[Ilxd2O7dHWg[Iln\mW{ZX70bYF1cW:wIHH0JFIhfU1iYX\0[ZIhOTRiZHH5d{BjgSCRaXygVoVlKE9ic4ThbY5qdmdibXX0bI9l NELyd4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjFxJ{7DbGVOSkx:L3G+
U2OS MV\GeY5kfGmxbjDhd5NigQ>? M{jFS|ExKHWP MXKyOEBpenN? MlPLRYN1cX[jdHnvckBw\iCSUFHSS{ApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCqdX3hckBWOk:VIHPlcIx{KGG2IEGwJJVOKGmwY4XiZZRm\CCob4KgNlQhcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigQ>? NGTwOYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjFxJ{7DbGVOSkx:L3G+
Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot Cyclin D1 / CDK2 / CDK4 / Bax / Bcl-2 29725405
Immunofluorescence PPARγ / E-cadherin 27145370
Growth inhibition assay Cell viability 29725405
In vivo

Rosiglitazone administration results in significant bone loss, including a decrease in bone volume, trabecular width, and trabecular number and an increase in trabecular spacing. Rosiglitazone also leads to a decrease in bone formation rate, with a simultaneous increase in fat content in the bone marrow. Rosiglitazone decreases the expression of the osteoblast-specific genes Runx2/Cbfa1, Dlx5, and alpha1(I)collagen, whereas the expression of the adipocyte-specific fatty acid binding protein aP2 is increased. [2] Rosiglitazone upregulates gene transcripts encoding mitochondrial proteins in white adipocytes from ob/ob mice accompanied by an increase in mitochondrial mass and changes in mitochondrial structure. [4]

Protocol (from reference)

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

 5mg/mL

Chemical Information

Molecular Weight 357.43
Formula

C18H19N3O3S
CAS No. 122320-73-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CN(CCOC1=CC=C(C=C1)CC2C(=O)NC(=O)S2)C3=CC=CC=N3

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02737709 Terminated Drug: Paclitaxel and Carboplatin regimen Non-small Cell Lung Cancer Sun Yat-sen University March 2016 Phase 2
NCT01402076 Completed Drug: Tasimelteon|Drug: Rosiglitazone|Drug: Midazolam Healthy Volunteers Vanda Pharmaceuticals August 2011 Phase 1
NCT01100619 Completed Drug: rosiglitazone|Drug: XL184 Papillary Thyroid Cancer|Follicular Thyroid Cancer|Huerthle Cell Thyroid Cancer|Renal Cell Carcinoma Exelixis April 2010 Phase 1
NCT01415128 Completed Drug: Omeprazole|Drug: Rosiglitazone|Drug: Desipramine Erectile Dysfunction VIVUS LLC April 2010 Phase 1
NCT01376063 Completed Drug: FG-4592 Healthy Adult Subjects FibroGen March 2010 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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