Rosiglitazone (BRL 49653)

Name: Rosiglitazone (BRL 49653)
Brand: Selleck Chemicals
SKU: S2556
Rating: 5 (36 reviews)

For research use only.

Catalog No.S2556

36 publications

Rosiglitazone (BRL 49653) Chemical Structure

CAS No. 122320-73-4

Rosiglitazone (BRL 49653) is a potent antihyperglycemic agent and a potent thiazolidinedione insulin sensitizer with IC50 of 12, 4 and 9 nM for rat, 3T3-L1 and human adipocytes, respectively. Rosiglitazone is a pure ligand of PPAR-gamma, and has no PPAR-alpha-binding action. Rosiglitazone modulates TRP channels and induces autophagy. Rosiglitazone prevents ferroptosis.

Selleck's Rosiglitazone (BRL 49653) has been cited by 36 publications

Nat Med, 2018, 24(3):360-367

PubMed: 29377004 ( click the link to review the publication )

Antioxidants (Basel), 2021, 10(2)155

PubMed: 33494520 ( click the link to review the publication )

Nat Commun, 2020, 11(1):71

PubMed: 31900415 ( click the link to review the publication )

Cell Death Dis, 2020, 11(9):752

PubMed: 32929062 ( click the link to review the publication )

FASEB J, 2020, 10.1096/fj.202001758R

PubMed: 33070393 ( click the link to review the publication )

Cancer Cell Int, 2020, 19;20:58

PubMed: 32099531 ( click the link to review the publication )

Exp Cell Res, 2020, S0014-4827(20)30690-X

PubMed: 33385414 ( click the link to review the publication )

Cancer Chemother Pharmacol, 2020, 85(2):273-284

PubMed: 31907647 ( click the link to review the publication )

Hum Cell, 2020, 10.1007/s13577-020-00420-z

PubMed: 32870449 ( click the link to review the publication )

Hum Cell, 2020, 10.1007/s13577-020-00425-8

PubMed: 32886306 ( click the link to review the publication )

Bioengineered, 2020, 11(1):1350-1367

PubMed: 33289586 ( click the link to review the publication )

Mol Omics, 2020, 16(6):533-542

PubMed: 33315023 ( click the link to review the publication )

Cell Death Differ, 2019, 10.1038/s41418-019-0299-4

PubMed: 30737476 ( click the link to review the publication )

Neuroimage, 2019, 10.1016/j.neuroimage.2019.05.069

PubMed: 31158477 ( click the link to review the publication )

J Cell Mol Med, 2019, 23(11):7406-7416

PubMed: 31475784 ( click the link to review the publication )

Mediators Inflamm, 2019, 2019:1769374

PubMed: 31772499 ( click the link to review the publication )

BMC Cancer, 2019, 19(1):204

PubMed: 30845932 ( click the link to review the publication )

Exp Biol Med (Maywood), 2019, 244(3):252-261

PubMed: 30714402 ( click the link to review the publication )

Ann Transl Med, 2018, 6(8):152

PubMed: 29862241 ( click the link to review the publication )

Toxicol Appl Pharmacol, 2018, 355:112-126

PubMed: 29782964 ( click the link to review the publication )

Theranostics, 2018, 8(15):4262-4278

PubMed: 30128052 ( click the link to review the publication )

Aging Cell, 2018, 10.1111/acel.12774

PubMed: 29740932 ( click the link to review the publication )

Cancer Sci, 2018, 109(7):2243-2255

PubMed: 29791764 ( click the link to review the publication )

PPAR Res, 2018, 2018:8512745

PubMed: 29686698 ( click the link to review the publication )
Int Immunopharmacol, 2018, 56:90-97

PubMed: 29367091 ( click the link to review the publication )

Process Biochemistry, 2018, 10.1016/j.procbio.2018.01.008

PubMed: ( click the link to review the publication )

Med Sci Monit, 2018, 24:6200-6207

PubMed: 30185768 ( click the link to review the publication )

Clin Exp Immunol, 2017, 189(2):211-225

PubMed: 28369855 ( click the link to review the publication )

Reprod Sci, 2017, 24(12):1590-1599

PubMed: 28322133 ( click the link to review the publication )

Oncol Rep, 2017, 38(5):3220-3226

PubMed: 28901466 ( click the link to review the publication )

Oncotarget, 2017, 8(56):95914-95930

PubMed: 29221176 ( click the link to review the publication )

J Immunol, 2016, 196(3):1044-59

PubMed: 26740111 ( click the link to review the publication )

RSC Advances, 2016, 10.1039/c6ra05637g

PubMed: ( click the link to review the publication )

Toxicol Appl Pharmacol, 2015, 285(1):51-60

PubMed: 25841593 ( click the link to review the publication )

J Leukoc Biol, 2014, 95(4):587-98

PubMed: 24338629 ( click the link to review the publication )

Stroke, 2013, 44(12):3498-508

PubMed: 24135932 ( click the link to review the publication )

Purity & Quality Control

Choose Selective PPAR Inhibitors

Biological Activity

Description

Targets

PPAR ^[1]
(Cell-free assay)

TRPC ^[5]
()

Ferroptosis ^[6]
()

In vitro

Rosiglitazone markedly increase phosphorylation of threonine 172 within the α subunit of AMPK, with increased AMP:ATP ratio. Rosiglitazone has been reported to decrease cholesterol synthesis in a number of cell lines in a peroxisome proliferator-activated receptor γ-independent manner. Rosiglitazone activates both α1- and α2-containing AMPK complexes, and this leads to a marked increase in the phosphorylation of acetyl-CoA carboxylase. ^[1] Rosiglitazone activates PPAR-gamma2 which acts as a dominant inhibitor of osteoblastogenesis in murine bone marrow in vitro.^[2] Rosiglitazone increases adiponectin secretion from omental cells up to 2.3-fold higher, whereas secretion from subcutaneous adipose cells is unaffected. ^[3] Rosiglitazone changes the morphological features and protein profiles of mitochondria in 3T3-L1 adipocytes. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
HepG2	M4L0RWZ2dmO2aX;uJIF{e2G7			MXTF[oZm[3SrdnWgZ49v[2WwdILheIlwdiCjZ3HpcpN1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSHXwS\|Ih[2WubIOsJGVEPTB;MD6wNkDPxE1?	M4joVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OECxPFE4Lz5zNUiwNVgyPzxxYU6=
CV-1	M175OGZ2dmO2aX;uJIF{e2G7			NEHYNplKdiC4aYTyc{B1emGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBw\iCSZYLvfIl{d22nIIDyc4xq\mW{YYTvdkBi[3SrdnH0[YQhemWlZYD0c5Ih\2GvbXGgLHBRSVJrIHX4dJJme3OnZDDpckBEXi1zIHPlcIx{NCCHQ{WwJF0hOC5yNjFOwG0v	NEfOO3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96NUe2PVA4Lz56NUe2PVA4RC:jPh?=
CV-1	NWTPd21xTnWwY4Tpc44h[XO\|YYm=			NE\ZbGNC[3SrdnH0bY9vKG:oIIDldo95cXOxbXWgdJJwdGmoZYLheI9zKGGldHn2ZZRm\CC{ZXPldJRweiCpYX3tZUBu\WG\|dYLl[EBjgSCrbnT1Z5Rqd25ib3[gOVAmKG:oIH3hfIlufW1iYXzrZYxqdmVicHjvd5Bp[XSjc3WgZYN1cX[rdImsJJRz[W6\|ZnXjeIlwdiCjc4PhfUBqdiCFVj2xJINmdGy\|LDDFR\|UxKD1iMD6wPFkyOyEQvF2u	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86QDN4NkKwK\|46QDN4NkKwQE9iRg>?
3T3-L1	NYDC[IE1TnWwY4Tpc44h[XO\|YYm=			MnzTV5RqdXWuYYTpc44hd2ZiYXTpdI9o\W6nc3nzJIlvKDOWMz3MNUBk\WyuczDpd{BmgHC{ZYPz[YQh[XNiY3;uZ4VvfHKjdHnvckBmeXWrdnHs[Y51KHSxIITo[UBcKDFvMUTDYUB2eHSja3WgZ492dnS\|IHHmeIVzKHS{ZXH0cYVvfCC5aYToJFAvOiC3Zz;tUEB1em:pbHn0ZZpwdmVuIFXDJF0hOC5yOTFOwG0v	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODl3NkKxN{c,OTB7NU[yNVM9N2F-
CV-1	NGXrSWpHfW6ldHnvckBie3OjeR?=			NEfkR29O[XirbXHsJJJmeG:{dHXyJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUHXyc5hqe2:vZTDwdo9tcW[ncnH0c5Ih[WO2aY\heIVlKHKnY3XweI9zKGejbX3hJGdidDRiY3jpcYVzcWNiaX6geJJidnOrZX70cJkhfHKjboPm[YN1\WRiQ2[tNUBk\WyuczDifUBnfW6ldHnvcoFtKGG\|c3H5MkwhTUN3MDC9JFAvODR\|IN88UU4>	NFnhO5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUeyNFg2PCd-MUG3NlA5PTR:L3G+
COS1	NY\2e4hHTnWwY4Tpc44h[XO\|YYm=			MmT6RYdwdmm\|dHnjJIFkfGm4aYT5JJdieyCmZYTldo1qdmWmIHnuJGNQWzFiY3XscJMhfHKjboPm[YN1\WRid3n0bEBISUxiND3QVGFTKGejbX3hJJJm[2WydH;yMEBGSzVyIE2gNE4xOjFizszNMi=>	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjZzN{myOEc,OTJ4MUe5NlQ9N2F-
COS1	NYr3R3FrTnWwY4Tpc44h[XO\|YYm=			NIDKTVdKdiC4aYTyc{Bi\2:waYP0JIFkfGm4aYT5JJRme3SnZDDmc5IhfHKjboPhZ5RqfmG2aX;uJIlvKGi3bXHuJHBRSVJiZ3HtcYEuT2GuNDDjbIlu\XKrYzDDU3MuOSClZXzsd{whTUN3MDC9JFAvODJizszNMi=>	M33wU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{N{K5OlY5Lz5zMkeyPVY3QDxxYU6=
COS1	NYXBdXgxTnWwY4Tpc44h[XO\|YYm=			NGXwUXNKdiC4aYTyc{B1emGwc3HjeIl3[XSrb36gc4YhcHWvYX6gdIVzd3irc3;t[UBxem:uaX\ldoF1d3JiYXP0bZZifGWmIILlZ4VxfG:{IHfhcY1iKG2nYYP1doVlKGmwIGDQRXIuT0GONDDjbIlu\XKrYzDDU3MuOSClZXzsd{whTUN3MDC9JFAvODJizszNMi=>	M3\nSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OEezOVE4Lz5zMki3N\|UyPzxxYU6=
CV-1	NV64TJFNTnWwY4Tpc44h[XO\|YYm=			M{HuOGlvKH[rdILvJIV3[Wy3YYTpc44h[WejaX7zeEBTYFJvYXzwbIEwWFCDUj3nZY1u[SCrbjDDWk0yKGOnbHzzJIJ6KGOxdILhcpNn\WO2aX;uJIF{e2G7IIfhd{Bl\XSncn3pcoVlNCCHQ{WwJF0hOC5\|MkWg{txONg>?	MlvCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ7NUSwOlEoRjF{OUW0NFYyRC:jPh?=
3T3-L1	MX\GeY5kfGmxbjDhd5NigQ>?			MXXF[oZm[3SrdnWgZ49v[2WwdILheIlwdiCob4Kg[Y5p[W6lZX3lcpQhd2ZiaX7zeYxqdi2rbnT1Z4VlKHS{aXfsfYNmemmmZTDhZ4N2dXWuYYTpc44hcW5iM2SzMWwyKGOnbHzzMEBGSzNyIE2gNE4xOiEQvF2u	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDVyNU[0OEc,OTR3MEW2OFQ9N2F-
HepG2	NFWxRnRHfW6ldHnvckBie3OjeR?=			NHPTVIpG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDh[4FqdnO2IHj1cYFvKFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTIVxTzJiY3XscJMtKEWFNUCgQUAxNjB\|OTFOwG0v	M3ztNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4MUC3NVUxLz5zNkGwO\|E2ODxxYU6=
U2OS	NWDNUHdiTnWwY4Tpc44h[XO\|YYm=			MmfMSYZn\WO2IH;uJHBRSVKpYX3tZUB1emGwc3HjeIl3[XSrb36gZYN1cX[rdImgbY4hXTKRUzDj[YxteyxiRVO1NEA:KDBwMEOg{txONg>?	NFLGZ3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkOwNFk1PCd-MU[zNFA6PDR:L3G+
Huh7	NY\q[5EzTnWwY4Tpc44h[XO\|YYm=			MU\GeY5kfGmxbnHsJIFkfGm4aYT5JIF1KGi3bXHuJHBRSVJiZ3HtcYEhcW5iSIXoO{Bk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkKyJO69VS5?	NWDDNndFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[zOlY3ODFpPkG2N\|Y3PjBzPD;hQi=>
Huh7	NX;EZ2s{TnWwY4Tpc44h[XO\|YYm=			MkfsSYZn\WO2IH;uJHBRSVJiZ3HtcYEhfHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JIlvKEi3aEegZ4VtdHNuIFXDOVAhRSByLkKyJO69VS5?	NXnPO\|ZjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0OVExQDdpPkG2OFUyODh5PD;hQi=>
HEK293	MmS0SpVv[3Srb36gZZN{[Xl?			MVfB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W\|c3XkJIF{KGmwZIXjeIlwdiCxZjDy[YNmeHSxcjDpcpRmemGldHnvckB4cXSqIIP0[ZJwcWRicnXj[ZB1d3JiY3;hZ5RqfmG2b4KtNUBjgSCHWV\QJIJie2WmIILldI9zfGW{IHflcoUh[XO\|YYm=	NVz3ZW4xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[2PFAyPTlpPkG2OlgxOTV7PD;hQi=>
HEK293	M3nmcGZ2dmO2aX;uJIF{e2G7			NV:1UVYzSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhemWlZYD0c5IhcW62ZYLhZ5Rqd25id3n0bEBz\XSrbn;p[EBZNXKnY3XweI9zKGGucHjhJIJ6KEW\RmCgZoF{\WRicnXwc5J1\XJiZ3Xu[UBie3OjeR?=	NVLYeZUyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[2PFAyPTlpPkG2OlgxOTV7PD;hQi=>
CV1	MUPGeY5kfGmxbjDhd5NigQ>?			M1Xxd3Rz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5iQ2[xJINmdGy\|LDDFR\|UxKD1iMD6wO\|Yh\|ryPLh?=	NEW0cWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkiyNVc3QSd-MU[4NlE4Pjl:L3G+
NIH3T3	M2TOdGZ2dmO2aX;uJIF{e2G7			MWPBZ5Rqfmm2eTDheEBpfW2jbjDQVGFTKGejbX3hJJRz[W6\|ZnXjeIVlKGmwIF7JTFNVOyClZXzsd{BjgSCudXPp[oVz[XOnIHHjeIl3cXS7IHHzd4F6NCCHQ{WwJF0hOC5\|MjFOwG0v	M{HQW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OEW0NFg2Lz5zNki1OFA5PTxxYU6=
CV1	NVHvU2FPTnWwY4Tpc44h[XO\|YYm=			MnHxWJJidnOjY4TpeoF1cW:wIH;mJIh2dWGwIGDQRXJo[W2vYTDpckBEXjFiY3XscJMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4{ODhizszNMi=>	M17SWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUewN\|kyLz5zNkm3NFM6OTxxYU6=
HepG2	MYfGeY5kfGmxbjDhd5NigQ>?			M2P2V2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWiCpYX3tZUBqdiCjIFjldGczKGOnbHzzJIJ6KFCSQWKtS2FNPCC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlE2QCEQvF2u	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl5M{O1PEc,OTZ7N{OzOVg9N2F-
HepG2	M4TBRmZ2dmO2aX;uJIF{e2G7			MV7B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXIh\2GvbXGgbY4hUGWyR{KgZ4VtdHNiYomgVHBCWi2JQVy0JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMUW4JO69VS5?	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl5OUO0NUc,OTZ7N{mzOFE9N2F-
CV1	M3fjZmZ2dmO2aX;uJIF{e2G7			MWfBZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgbY4hS1ZzIHPlcIx{NCCHQ{WwJF0hOC5\|MEig{txONg>?	MlP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdyMEWzPVQoRjF5MEC1N\|k1RC:jPh?=
Huh7	MnTGSpVv[3Srb36gZZN{[Xl?			M2DlWGFkfGm4aYT5JIF1KGi3bXHuJHBRSVJiZ3HtcYEhcW5iSIXoO{Bk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkKyJO69VS5?	NU\1WoNlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUewN\|QyPDlpPkG3NFM1OTR7PD;hQi=>
CV1	MWXGeY5kfGmxbjDhd5NigQ>?			MkPPRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhcW5iQ2[xJINmdGy\|IHL5JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwM{C4JO69VS5?	NUXCe5lpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexNlk4OjVpPkG3NVI6PzJ3PD;hQi=>
HepG2	MYrGeY5kfGmxbjDhd5NigQ>?			NX3YN5U1SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEhcW5iSHXwS\|Ih[2WubIOgZpkhWFCDUj3HRWw1KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNFgzKM7:TT6=	M{T1T\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUW3NFE6Lz5zN{G1O\|AyQTxxYU6=
HEK293	NHrUdGFHfW6ldHnvckBie3OjeR?=			NG[3NWVC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SC{ZXPldJRweiCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGK7IFfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6wN\|Uh\|ryPLh?=	NWO0fHpoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUeyPVI3ODZpPkG3NlkzPjB4PD;hQi=>
HEK293	MoLqSpVv[3Srb36gZZN{[Xl?			MnT3RYN1cX[rdImgZZQhcHWvYX6gZYRqeG:\|ZTD0bZN{fWViUGDBVkBo[W2vYTDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHL5JHBRSVJvR1HMOEB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkOxJO69VS5?	NIjoWZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{O0N\|M4OSd-MUezOFM{PzF:L3G+
L6	NX:4V2dOTnWwY4Tpc44h[XO\|YYm=			MX3F[oZm[3Rib36g[oF1fHliYXPp[EBwgGmmYYTpc44hcW5icnH0JGw3KGOnbHzzMEBGSzVyIE2gOUDPxE1w	NYLnNmRyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezOFM{PzFpPkG3N\|Q{OzdzPD;hQi=>
HepG2/C3A	NGnOcI1HfW6ldHnvckBie3OjeR?=			MkCyRYN1cX[jdHnvckBw\iCqdX3hckBRWEGUZ3HtcYEhdGmpYX7kJIJqdmSrbneg[I9u[WmwLX3l[IlifGWmIITyZY5{[3KrcITpc45idCCjY4Tpeol1gSCrbjDoeY1idiCKZYDHNk9EO0FiY3XscJMh[29vdILhcpNn\WO2ZXSge4l1cCCodYPl[EBI[Wx2LVzCSEBjgSClb4TyZY5{\mWldHnvckBie3OjeTygSWM2OCB;IECuNFA{QCEQvF2u	NEH5UJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SwN\|Y5QCd-MUe0NFM3QDh:L3G+
CV1	M3LmVWZ2dmO2aX;uJIF{e2G7		M1nKflI1KGi{cx?=	M2rJXmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIlvKEOYMTDj[YxteyCkeTDHRWw1KHS{YX7zZYN1cX[jdHnvckBie3OjeTDh[pRmeiB{NDDodpMtKEWFNUCgQUAxNjB\|IN88UU4>	M1fDeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUC3NlI2Lz5zN{WwO\|IzPTxxYU6=
CV1	NF3vWplHfW6ldHnvckBie3OjeR?=		NUDSVphCOjRiaILz	NX;wXHpGSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUYXzwbIEhcW5iQ2[xJINmdGy\|IHL5JGdCVDRidILhcpNi[3SrdnH0bY9vKGG\|c3H5JIFnfGW{IEK0JIhzeyxiRVO1NEA:KDNwNE[g{txONg>?	M{j0UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUC3NlI2Lz5zN{WwO\|IzPTxxYU6=
NIH3T3	NGP1ZopHfW6ldHnvckBie3OjeR?=			NHzUNIhC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iTlnIN3Q{KGOnbHzzJIJ6KEeDTESgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5\|MjFOwG0v	M37hVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkK0O\|c4Lz5zN{[yOFc4PzxxYU6=
HEK293	MkfmSpVv[3Srb36gZZN{[Xl?			MlSzRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{Bie3Onc4Pl[EBieyCjUEKg[4Vv\SCrbnT1Z5Rqd25uIFXDOVAhRSByLkGyJO69VS5?	NFjo[XE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOECyPVE4Pid-MUiwNlkyPzZ:L3G+
HEK293	MmDhSpVv[3Srb36gZZN{[Xl?			NYfhZ\|NpSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCkeTDHRWw1KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNVkh\|ryPLh?=	M{Cx[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MEK5NVc3Lz5zOECyPVE4PjxxYU6=
CV1	NWnGOW1jTnWwY4Tpc44h[XO\|YYm=			NImxfpJC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iQ2[xJINmdGy\|IHL5JJJm[2WydH;yJJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwM{C4JO69VS5?	M37pNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MEK5NVc5Lz5zOECyPVE4QDxxYU6=
HEK293	MUDGeY5kfGmxbjDhd5NigQ>?			M13WTGFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIFjFT\|I6OyClZXzsd{BjgSCJQVy0JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMES1JO69VS5?	NVPhWlhURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyPVE3PDVpPkG4NlkyPjR3PD;hQi=>
U2OS	NHrQXGJHfW6ldHnvckBie3OjeR?=			NUGwU3BmSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEhcW5iVULPV{Bk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkCyJO69VS5?	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN{OUe1NUc,OTh\|Mkm3OVE9N2F-
HepG2	MlLOSpVv[3Srb36gZZN{[Xl?			M4TmTWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCKZYDHNkBk\WyuczDifUBISUx2IITyZY5{[WO2aY\heIlwdiCjc4PhfUwhTUN3MDC9JFAvOjJ\|IN88UU4>	M1rBO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{m0PVA4Lz5zOEO5OFkxPzxxYU6=
HepG2	MVTGeY5kfGmxbjDhd5NigQ>?			MX7B[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKHKnY3XweI9zKHS{YX7zZYN1cX[jdHnvckBjgSC{ZYDvdpRmeiCpZX7lJIF{e2G7IILlcIF1cX[nIITvJINwdnS{b3ysJGVEPTBiPTCwMlA2KM7:TT6=	Mo\4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4MkW1OVkoRjF6NkK1OVU6RC:jPh?=
RAW264.7	MYPGeY5kfGmxbjDhd5NigQ>?			NHzo[XZUfXCycnXzd4lwdiCxZjDMVHMwUU[QLXfhcY1iNXO2aX31cIF1\WRiTl:gdJJw\HWldHnvckBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczygTWM2OCB;IEG3MlUh\|ryPLh?=	MlvwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh6MEmzNlUoRjF6OEC5N\|I2RC:jPh?=
CV1	M1L3[WZ2dmO2aX;uJIF{e2G7			MonyRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDh[pJq[2GwIHfy[YVvKG2xbnvlfUBEXjFiY3XscJMh[29vdILhcpNn\WO2ZXSge4l1cCCodYPl[EBI[Wx2LVTCSEBjgSC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlA{OyEQvF2u	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDh{NkKwOUc,OTh6Mk[yNFU9N2F-
CV1	NWq1WWpOTnWwY4Tpc44h[XO\|YYm=			M4nyeGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmGucHjhJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS1ZzIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiZoXz[YQhT2GuND3ERmQh[nlidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gN{41PiEQvF2u	MmrMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh6Mk[yNFUoRjF6OEK2NlA2RC:jPh?=
HepG2	M364eGZ2dmO2aX;uJIF{e2G7			MV;B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJidHCqYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIHj1cYFvKEincFeyJINmdGy\|IHPvMZRz[W6\|ZnXjeIVlKHerdHigS4FtPCCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjB\|OTFOwG0v	NV\UfnhIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4N\|U4OTlpPkG4PFM2PzF7PD;hQi=>
HepG2	MXPGeY5kfGmxbjDhd5NigQ>?			MnS4RYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDoeY1idiCKZYCgS\|Ih[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBI[Wx2LVTCSEBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlA1KM7:TT6=	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{e3Okc,OTlyNUO3O\|Y9N2F-
HeLa	MkjESpVv[3Srb36gZZN{[Xl?			NVW4cG1iSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFibHnnZY5lKGKrbnTpcoch\G:vYXnuJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\UyjIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiR3HsOE1FSkRiYYPz[ZN{\WRiYYOgeJJidnOlcnnweIlwdmGuIHHjeIl3[XSrb36gZpkhT2GuNDDy[ZNxd26\|ZTDlcIVu\W62LXTybZZmdiCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlAyPSEQvF2u	NUL3cJc2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmyO\|U6PjNpPkG5Nlc2QTZ\|PD;hQi=>
BL21	NYXBZnJ5TnWwY4Tpc44h[XO\|YYm=			MlK3SIl{eGyjY3Xt[Y51KG:oIILh[IlwKGyjYnXs[YQhOiiVKT2oNk1j\W68b4nsMZBp\W67bHHtbY5wMS1\|LYu0MXsyNDFvZHn0dol1cW9vMj2oOU1u\XSqeXytNk1xcGWweXytc5higm:uLUStfYwqNWW2aH;4fX0ueGinbonsgU1xem:yaX;ubYMh[WOrZDDmdo9uKEeVVD3meZNm\CCqdX3hckBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQlyyNUBk\WyuczDifUB{[2mwdHnscIF1cW:wIIDyc5hqdWm2eTDhd5NigSxiSVO1NEA:KDBwNEWg{txONg>?	M3LrcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{S5NVc3Lz5zOUO0PVE4PjxxYU6=
BHK21	MYDGeY5kfGmxbjDhd5NigQ>?			MUHB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO\|ZXSgbY4hSkiNMkGgZ4VtdHNiYYPz[ZN{\WRiYYOgV2VCWCCjY4Tpeol1gSCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwNEWg{txONg>?	M17GSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{S5NVc3Lz5zOUO0PVE4PjxxYU6=
COS1	M2DnXGZ2dmO2aX;uJIF{e2G7		M1foVlQ5KGi{cx?=	MWXB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIILlZ49u[mmwYX70JHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iQ1;TNUBk\WyuczDjc{1mgHC{ZYPzbY5oKEeDTESgZZN{\XO\|ZXSgZZMhfHKjboPjdolxfGmxbnHsJIFkfGm4aYT5JIFnfGW{IES4JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjB{IN88UU4>	NUm1e2VjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1NFc5PjFpPkG5OVA4QDZzPD;hQi=>
COS1	NE\T[pdHfW6ldHnvckBie3OjeR?=			MmLWRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgdoVk\XC2b4Kg[ZhxemW\|c3XkJIlvKGGocnnjZY4h\3KnZX6gcY9vc2W7IFPPV\|Eh[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBnfXOnZDD5[YF{fCCJYXy0MWRDTCCkeTD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjB{IN88UU4>	Ml\zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3M{C2PFEoRjF7NUOwOlgyRC:jPh?=
U2OS	M{CwfmZ2dmO2aX;uJIF{e2G7			NEPTOnpC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iVULPV{Bk\WyuczDifUBtfWOrZnXyZZNmKHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNFUh\|ryPLh?=	NUj3d4M2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1O\|QxPTZpPkG5OVc1ODV4PD;hQi=>
MCF7	MlT2SpVv[3Srb36gZZN{[Xl?	MlvDNE4yKHWP	MWGyOEBpenN?	NHTN[4FC[3SrdnH0bY9vKG:oIHj1cYFvKFCSQWKt[4FudWFvZHXw[Y5l\W62IITyZY5{[3KrcITpc44h\XiycnXzd4VlKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKFCSUlWtdoVxd3K2ZYKg[4Vv\SCneIDy[ZN{cW:wIHH0JFAvOSC3TTDh[pRmeiB{NDDodpMh[nliZIXhcEBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XlicnXsZZRqfmVidH:gdo9{cWeuaYThfo9v\Q>?	MkXVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl5MUmyN\|YoRjF7N{G5NlM3RC:jPh?=
HepG2	NEfHRWRHfW6ldHnvckBie3OjeR?=			Mn7vRYdwdmm\|dDDhZ5Rqfmm2eTDheEBISUx2LYTh[4dm\CCqdX3hckBRWEGUZ3HtcYEhdGmpYX7kJIJqdmSrbneg[I9u[WmwIHX4dJJme3OnZDDpckBpfW2jbjDI[ZBIOiClZXzsd{Bie3Onc4Pl[EBieyC{ZXPldJRweiC2cnHud4FkfGm4YYTpc44h[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4xOzlizszNMi=>	NITm[nI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUe3OVE3QSd-MUm3O\|UyPjl:L3G+
HepG2	M2focWZ2dmO2aX;uJIF{e2G7			Mon4RYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKEincFeyJINmdGy\|IHL5JGdCVDRidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gNE4zOjNizszNMi=>	MkPTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl5OEO0OFQoRjF7N{izOFQ1RC:jPh?=
COS7	M4S2VWZ2dmO2aX;uJIF{e2G7			MmjNRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDy[YNwdWKrbnHueEBRWEGUZ3HtcYEyKEyERDDlfJBz\XO\|ZXSgbY4h[W[{aXPhckBoemWnbjDtc45s\XliQ1;TO{Bk\WyuczDjc4V5eHKnc4PpcochT0GONDDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMzFOwG0v	M{DaOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUO4O\|YzLz5{MEGzPFc3OjxxYU6=
COS7	NHLPcJNHfW6ldHnvckBie3OjeR?=			M1vhZ2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gdoVkd22kaX7hcpQhWFCDUnfhcY1iOiCOQlSg[ZhxemW\|c3XkJIlvKGGocnnjZY4h\3KnZX6gcY9vc2W7IFPPV\|ch[2WubIOgZ49mgHC{ZYPzbY5oKEeDTESgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMTFOwG0v	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF\|OEe2Nkc,OjBzM{i3OlI9N2F-
HEK	MW\GeY5kfGmxbjDhd5NigQ>?			NFjseZpC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGdCVDRvdHHn[4VlKFCSQWLnZY1u[SClaHnt[ZJq[yC{ZXPldJRweiCneIDy[ZN{\WRiaX6gTGVMKGOnbHzzJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNFM2KM7:TT6=	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODJ7OUKxOEc,OjB{OUmyNVQ9N2F-
HEK	M3PCUmZ2dmO2aX;uJIF{e2G7			M3n4ZmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gS2FNPC22YXfn[YQhWFCDUnHsdIhiKGOqaX3ldolkKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWsh[2WubIOgZpkhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMUCuOVgh\|ryPLh?=	MlryQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB{OUmyNVQoRjJyMkm5NlE1RC:jPh?=
CHO-K1	MV\GeY5kfGmxbjDhd5NigQ>?			NF\HUGtR[XK2aXHsJIFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hMWxDTCCneIDy[ZN{\WRiaX6gR2hQNUtzIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiR1HMOEBie3Onc4Pl[EBieyCudXPp[oVz[XOnIHHjeIl3cXS7IHL5JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMTFOwG0v	M{LxdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNUK3PVY6Lz5{MEWyO\|k3QTxxYU6=
3T3L1	NHu5ZXZHfW6ldHnvckBie3OjeR?=		NGDhdJU4KGSjeYO=	MVXJcoR2[3Srb36gc4Yh[WSrcH;n[Y5me2m\|IHnuJI1wfXOnIEPUN2wyKGOnbHzzJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIHzpdIllKGGlY4XteYxifGmxbjDh[pRmeiB5IHThfZMh[nliT3nsJJJm\CCRIIP0ZYlvcW6p	MmHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3Mke5OlkoRjJyNUK3PVY6RC:jPh?=
HEK293T	Mk\iSpVv[3Srb36gZZN{[Xl?	NWHuWWx1OSC3TR?=	NGLEeYYzPCCqcoO=	NXTy[Vd[WGG{dHnhcEBi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZU1NSkRiZYjwdoV{e2WmIHnuJGhGUzJ7M2SgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKHKnY3XweI9zKHS{YX7zZYN1cX[jdHnvckBifCBzIIXNJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk>	NF;TZ3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUCzNFI3Oyd-MkGwN\|AzPjN:L3G+
CV1	NV:0eplMTnWwY4Tpc44h[XO\|YYm=			NYPKdmtFXHKjboPhZ5RqfmG2aX;uJI9nKEejbESt[pV{\WRiaIXtZY4hWFCDUnfhcY1iKESQQTDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS1ZzIHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvOSEQvF2u	NYPXO2FPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxNVI4QDRpPkKxNVEzPzh2PD;hQi=>
HEK293	NVzBUVBiTnWwY4Tpc44h[XO\|YYm=		M{DsTVE3KHSxIEKwJIhzew>?	M2PIN3Rz[W6\|YXP0bZZifGmxbjDv[kBI[Wx2LX\1d4VlKGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIFnfGW{IEG2JJRwKDJyIHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlA1OyEQvF2u	NXTOXJFTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxNlg3ODBpPkKxNVI5PjByPD;hQi=>
HepG2	Mkm2SpVv[3Srb36gZZN{[Xl?	NGjibVAyOCC3bX;sM2w>	MkX5NlQhcHK\|	NV3GbGZ4UW6lcnXhd4UhcW5iZ3z1Z49{\SClb37zeY1xfGmxbjDpckBpfW2jbjDI[ZBIOiClZXzsd{BifCBzMDD1cY9tN0xiYX\0[ZIhOjRiaILzJJJmdGG2aY\lJJRwKGOxboTyc4w>	M4TXU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkW2O\|Q5Lz5{MUK1Olc1QDxxYU6=
HepG2	NYX2NVBWTnWwY4Tpc44h[XO\|YYm=			NXXz[IppXHKjboPhZ5RqfmG2aX;uJI9nKGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiY3:teJJidnOoZXP0[YQhf2m2aDDQVHJGOy2WSz3MeYMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4xPSEQvF2u	NFvmRZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS1NFQ3QCd-MkG0OVA1Pjh:L3G+
HepG2	NH\5THpHfW6ldHnvckBie3OjeR?=			M13mOGFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKHKnY3XweI9zKGW6cILld5Nm\CCrbjDoeY1idiCKZYDHNkBk\WyuczDifUBRWFKHLXz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODFizszNMi=>	NHzKVFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS4NlQ1Pid-MkG0PFI1PDZ:L3G+
CV1	MWHGeY5kfGmxbjDhd5NigQ>?		NGLDdZc1OCCqcoO=	MYDB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYT3MRmQh\XiycnXzd4VlKGmwIFPWNUBk\WyuczDjc{11emGwc3\lZ5Rm\CC5aYToJGdidDRiYX\0[ZIhPDBiaILzJIJ6KGy3Y3nm[ZJie2ViYnHz[YQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6xJO69VS5?	NEHoVWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxOVA3Oyd-MkG1NVUxPjN:L3G+
3T3L1	NFTHe\|dHfW6ldHnvckBie3OjeR?=	M{nXfVExKHWP		MUfJcoNz\WG\|ZTDpckBi\Gmyb37lZ5RqdiCvUl7BJIxmfmWuczDpckBVVk[jbIDoZU1qdmS3Y3XkJI1wfXOnIEPUN2wyKGOnbHzzJJBz\XS{ZXH0[YQh[XRiMUCgeW0h[mWob4LlJHRPTi2jbIDoZUBkcGGubHXu[4UhemWuYYTpeoUhfG9iY3;ueJJwdA>?	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh3MUC4PUc,OjF6NUGwPFk9N2F-
COS7	MXnGeY5kfGmxbjDhd5NigQ>?			NGf2bmlOd2S3bHH0bY9vKG:oIHj1cYFvKFCSQWLnZY1u[S2OQlSg[ZhxemW\|c3XkJIlvKGGocnnjZY4h\3KnZX6gcY9vc2W7IFPPV\|ch[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBI[Wx2IHHzd4V{e2WmIHHzJIFkfGm4YYTpc44hd2ZidILhcpNi[3SrdnH0bY9vKGGldHn2bZR6KGK7IHz1Z4ln\XKjc3WgZZN{[XluIFXDOVAhRSByLkC0N{DPxE1w	MorLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{OwO\|AoRjJzOEezNFcxRC:jPh?=
Sf21	NGrsUXVHfW6ldHnvckBie3OjeR?=			NEfIeXZKdmirYnn0bY9vKG:oIHj1cYFvKEKVRWCg[ZhxemW\|c3XkJIlvKHCuYYPtZUBu\W2kcnHu[UB3\XOrY3zld{Bw\iCVZkKxJINmdGy\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiQWTQMYRmeGWwZHXueEBcO0ifdHH1do9kcG:uYYTlJJVxfGGtZTygTWM2OCB;IE[uOEDPxE1w	M3XOUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOU[1OlI{Lz5{MUm2OVYzOzxxYU6=
Sf21	NFq3[FlHfW6ldHnvckBie3OjeR?=			NXf3d2JOUW6qaXLpeIlwdiCxZjDTdJJi\3WnLVThe4xmgSC{YYSgRpNmeCCneIDy[ZN{\WRiaX6gdIxie22jIH3lcYJz[W6nII\ld4lkdGW\|IH;mJHNnOjFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBCXFBvZHXw[Y5l\W62IGuzTH11[XW{b3Poc4xifGVidYD0ZYtmNCCLQ{WwJF0hPDBizszNMi=>	Moi1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NkW2NlMoRjJzOU[1OlI{RC:jPh?=
COS-1	MnTZSpVv[3Srb36gZZN{[Xl?		NIPmT4szPCCqcoO=	NHzkcY5C\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJGNQWy1zIHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWtZoF{\WRibIXtbY5wdWW2cnnjJIFv[Wy7c3nzMEBGSzVyIE2gNE4xPDhizszNMi=>	M3LRVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEWxNFU1Lz5{MkC1NVA2PDxxYU6=
COS1	MYLGeY5kfGmxbjDhd5NigQ>?			MoDZRolv\GmwZzDh[oZqdmm2eTD0c{BpfW2jbjD3bYxlKHS7cHWgVHBCWmejbX3hJGxDTCCneIDy[ZN{\WRiaX6gR29UOSClZXzsd{Bkdy2neIDy[ZN{cW6pIFfBUFQh[nlic3PpcpRqdGyjdHnvckBxem:6aX3peJkh[XO\|YYm=	MkXxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyN{C2NFQoRjJ{MEewOlA1RC:jPh?=
HepG2	M{nISWZ2dmO2aX;uJIF{e2G7		NVG1d5Y1OjBiaILz	NWXUbHhrSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBISUx2LX\1d4VlKFCSQWLnZY1u[SCuaXfhcoQh[mmwZHnu[{Bld22jaX6g[ZhxemW\|c3XkJIlvKEincFeyJINmdGy\|IHHmeIVzKDJyIHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6wNkDPxE1w	MnLoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyOEG5N\|IoRjJ{MEixPVMzRC:jPh?=
BL21 DE3	M2fSZ2Z2dmO2aX;uJIF{e2G7			NXT2fVJlTGm\|cHzhZ4Vu\W62IH;mJHs{UF2{b4Pp[4xqfGG8b37lJIZzd21iTj30[ZJucW6jbDDIbZMufGGpZ3XkJIh2dWGwIGDQRXJo[W2vYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQlyyNUBFTTNiY3XscJMh[nlic3PpcpRqdGyjdHnvckBxem:6aX3peJkh[XO\|YYmsJGtqKD1iMD6wO\|Qh\|ryPLh?=	NFnaO5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC4NVk{Oid-MkKwPFE6OzJ:L3G+
COS1	M1nvUmZ2dmO2aX;uJIF{e2G7		M3HnNVI1KGi{cx?=	MlfNWJJidnOjY4TpeoF1cW:wIH;mJIh2dWGwIH\1cIwhdGWwZ4ToJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iQ1;TNUBk\WyuczDjc{11emGwc3\lZ5Rm\CC5aYToJHJZWmGucHjhJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjF{IN88UU4>	M{LCflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUm3N\|k3Lz5{MkG5O\|M6PjxxYU6=
MG-63	NUGxT3FITnWwY4Tpc44h[XO\|YYm=			MoDPRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKE2JLU[zJINmdGy\|IHL5JJJmeG:{dHXyJIdmdmVvYnHz[YQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6wNVEh\|ryPLh?=	M2PUN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkK1OlQyLz5{MkKyOVY1OTxxYU6=
293H DA	M2nVe2Z2dmO2aX;uJIF{e2G7		MWexOkBpenN?	NWfGbJFpSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiTFLEJIlvKGi3bXHuJFI6O0hiRFGgZ4VtdHNiYX\0[ZIhOTZiaILzJIJ6KFSULV\SSXQh[WO2aY\heIlwdiC{ZYDvdpRmeiCjc4PhfUwhTUN3MDC9JFAvODB{NDFOwG0v	NIPSWIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW4Nlk4Oyd-MkK1PFI6PzN:L3G+
MG-63	NXfwfIh{TnWwY4Tpc44h[XO\|YYm=		NUDmWoRpOjRiaILz	NIPSZlNOd2S3bHH0bY9vKG:oIH\1cIwudGWwZ4ToJIh2dWGwIIDTS\|Uu\nW\|ZXSgVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCPRz22N{Bk\WyuczDjc{1mgHC{ZYPzbY5oKHCJVj3QNk1RWFKHIHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[mG\|ZXSgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMUGg{txONg>?	NYnJU3cyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3Nlc1PDhpPkKyO\|I4PDR6PD;hQi=>
Ac2F	NV7z[pZ5TnWwY4Tpc44h[XO\|YYm=	MmL1NE4yKHSxIEGwJJVO	MlvZOkBpenN?	MX;B[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiC{YYSgRYMzTiClZXzsd{Bie3Onc4Pl[EBieyCudXPp[oVz[XOnIHHjeIl3cXS7IHH0JFAvOSC2bzCxNEB2VSCjZoTldkA3KGi{czDifUBz\XCxcoTldkBo\W6nIHHzd4F6	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjhzOUG5NEc,OjJ6MUmxPVA9N2F-
HepG2	NFHpRlZHfW6ldHnvckBie3OjeR?=			MmXDWJJidnOjY4TpeoF1cW:wIH;mJGdCVDRvZoXz[YQhWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjD0doFve2[nY4Tl[EBqdiCqdX3hckBJ\XCJMjDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjFizszNMi=>	NWj4ZmZZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5N\|Q2OzdpPkKyPVM1PTN5PD;hQi=>
THP1	MlrBSpVv[3Srb36gZZN{[Xl?	MWqxNFAhfU1?	NYn6VFJ{OyCqcoO=	NWXVO3V1XHKjboPhZ5RqfmG2aX;uJI9nKFCSQWLnZY1u[SCrbjDoeY1idiCWSGCxJINmdGy\|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKEySUz3pcoR2[2WmIF3DVFEhdVKQQTDlfJBz\XO\|aX;uJIF1KDFyMDD1UUBxemWrbnP1ZoF1\WRiZn;yJFMhcHK\|IIDybY9zKHSxIFzQV{BkcGGubHXu[4UhdWWjc4Xy[YQh[W[2ZYKgNVghcHK\|IHL5JHJVNVCFUjDhcoFtgXOrczDy[YxifGm4ZTD0c{B2dnS{ZXH0[YQh[2:wdILvcC=>	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl\|NEWzO{c,OjJ7M{S1N\|c9N2F-
THP1	MmTrSpVv[3Srb36gZZN{[Xl?	Mn3ZNVAxKHWP	MWqzJIhzew>?	M3vWeXRz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5iaIXtZY4hXEiSMTDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG\|ZTDpckBNWFNvaX7keYNm\CCLTE[gcXJPSSCneIDy[ZN{cW:wIHH0JFExOCC3TTDwdoVqdmO3YnH0[YQh\m:{IEOgbJJ{KHC{aX;yJJRwKEySUzDjbIFtdGWwZ3WgcYVie3W{ZXSgZYZ1\XJiMUigbJJ{KGK7IGLUMXBEWiCjbnHsfZNqeyC{ZXzheIl3\SC2bzD1cpRz\WG2ZXSgZ49vfHKxbB?=	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl\|NEWzO{c,OjJ7M{S1N\|c9N2F-
HepG2	NVzaRW91TnWwY4Tpc44h[XO\|YYm=	M1fUcVExKHWP	M3SxcFI1KGi{cx?=	NYC4eHlHWmWmdXP0bY9vKG:oIHfseYNwe2ViY3;ud5VueHSrb36gbY4hcW6\|dXzpck1z\XOrc4ThcpQhcHWvYX6gTIVxTzJiY3XscJMh[XRiMUCgeW0h[W[2ZYKgNlQhcHK\|IHL5JIdtfWOxc3Wgc5hq\GG\|ZTDt[ZRpd2RiaX6gdJJme2WwY3Wgc4YhOC5zIIXNJI9nKGmwc4XsbY4>	M124[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEK1NlQ1Lz5{M{CyOVI1PDxxYU6=
HepG2	MmrYSpVv[3Srb36gZZN{[Xl?	MYexNEB2VQ>?	NEe0XmQzPCCqcoO=	M{S5[nJm\HWldHnvckBw\iCpbIXjc5NmKGOxboP1cZB1cW:wIHnuJIlve3WuaX6tdoV{cXO2YX70JIh2dWGwIFjldGczKGOnbHzzJIF1KDFyIIXNJIFnfGW{IEK0JIhzeyCkeTDncJVkd3OnIH;4bYRie2VibXX0bI9lKGmwIIDy[ZNmdmOnIH;mJFIzNjJibV2gc4Yh\2y3Y3;z[S=>	NH;n[lQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CyOVI1PCd-MkOwNlUzPDR:L3G+
HepG2	NILadJZHfW6ldHnvckBie3OjeR?=		MoCxNlAhcHK\|	M1z1U3Rz[W6\|YXP0bZZifGmxbjDv[kBISUx2LX\1d4VlKGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJGhmeEd{IHPlcIx{KGGodHXyJFIxKGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCzPUDPxE1w	NUf6XJJoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxO\|ExPDVpPkKzNVcyODR3PD;hQi=>
L02	Mlu3SpVv[3Srb36gZZN{[Xl?		MlvQNlQhcHK\|	MlT5RYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEuVEKGIHX4dJJme3OnZDDpckBpfW2jbjDMNFIh[2WubIOgZ48u\XiycnXzd4lv\yCyR1yzMXNXPDBvR1HMOEBi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHLhd4VlKHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuPUDPxE1w	M3\CdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MUi2N\|A4Lz5{M{G4OlMxPzxxYU6=
HEK293	MojTSpVv[3Srb36gZZN{[Xl?		NEi4bGQ1QCCqcoO=	M3Ttd2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZ491emGwc3\lZ5Rm\CC5aYToJHBRWkW6ND3UT{1tfWNiYYPz[ZN{\WRiYYOgZYN1cX[jdHnvckBw\iCudXPp[oVz[XOnIHHjeIl3cXS7IH3lZZN2emWmIHHmeIVzKDR6IHjyd{BjgSC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTC0N{44OSEQvF2u	NF[yOng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K2OVg1PCd-MkOyOlU5PDR:L3G+
HEK293	MW\GeY5kfGmxbjDhd5NigQ>?	M1ntPFExKHWP		NEPDcYlVemGwc3HjeIl3[XSrb36gc4YhWFCDUnfhcY1iKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKEGSMj3QVHJGKGG2IEGwJJVOKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	NUn3N3NwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyO\|M2OTlpPkKzNlc{PTF7PD;hQi=>
HEK293	MkXJSpVv[3Srb36gZZN{[Xl?			M{TVcGFod26rc4SgZZQhcHWvYX6gVHBCWmejbX3hJGxDTCCneIDy[ZN{\WRiaX6gbJVu[W5iSFXLNlk{KGOnbHzzJINwfHKjboPm[YN1\WRid3n0bEBISUx2LVz1Z{Bie3Onc4Pl[EBieyC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjByNDFOwG0v	M{nIN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Mki2O\|g4Lz5{M{K4Olc5PzxxYU6=
HEK293	NFXHPY5HfW6ldHnvckBie3OjeR?=		MVOyOEBpenN?	MkHIWJJidnOjY4TpeoF1cW:wIH;mJGdCVDRiRFLEMYZ2e2WmIHj1cYFvKFCSQWLnZY1u[S2OQlSg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4yKM7:TT6=	Mkj4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OUS4N\|AoRjJ\|Mkm0PFMxRC:jPh?=
CMT93	Mn;MSpVv[3Srb36gZZN{[Xl?	M3T3ZVExKHWP	NFvPeIw{OCCvaX7z	MXjJcoR2[3Srb36gc4YhWFCDUj3nZY1u[SCycn;tc5RmeiCjY4Tpeol1gSCrbjDMVHMue3SrbYXsZZRm\CCvb4Xz[UBEVVR7MzDj[YxteyCjdDCxNEB2VSCycnXpcoN2[mG2ZXSg[o9zKDNyIH3pcpMh[mWob4LlJGxRWy2laHHscIVv\2VibXXhd5Vz\WRiYX\0[ZIhPiCqcoOgdI9{fCClaHHscIVv\2ViYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6	NH;pPVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SxOlE6OCd-MkO0NVYyQTB:L3G+
HepG2	MUDGeY5kfGmxbjDhd5NigQ>?			NV30UoM2SWexbnnzeEBi[3Srdnn0fUBifCCJQVy0MYZ2e2WmIGDQRXJo[W2vYTDNOFY{SSCvdYThcpQhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiYomgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMEKg{txONg>?	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzVyMkKxNkc,OjN3MEKyNVI9N2F-
HepG2	NHPCNolHfW6ldHnvckBie3OjeR?=			MV3B[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRvZoXz[YQhWFCDUnfhcY1iKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIHj1cYFvKEincFeyJINmdGy\|IHL5JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEGg{txONg>?	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzVyMkKxNkc,OjN3MEKyNVI9N2F-
L6	MXfGeY5kfGmxbjDhd5NigQ>?		MVmyOEBpenN?	NFTye5BKdmS3Y4Tpc44hd2ZiZ3z1Z49{\SC3cIThb4UhcW5icnH0JGw3KGOnbHzzJJB2dHOnZDD3bZRpKENzND3k[Y95gSCpbIXjc5NmKGGodHXyJFI1KGi{czDpckBxemW\|ZX7j[UBw\iCrboP1cIlvNCCHQ{WwJF0hPC52OTFOwG0v	NUW1TYNRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5PVI5PjJpPkKzPVkzQDZ{PD;hQi=>
L6	M1f5cGZ2dmO2aX;uJIF{e2G7		NYfFOVg4OTZiaILz	NH7OSllKdmO{ZXHz[UBqdiB{LWuzTH0u\GWxeInncJVkd3OnIIXweIFs\SCrbjDyZZQhVDZiY3XscJMh[W[2ZYKgNVYhcHK\|IHL5JJNkcW62aXzsZZRqd25iY3;1cpRqdmdiYX7hcJl{cXNuIFnDOVAhRSB2Lkig{txONg>?	MmjaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUO3N\|goRjJ2OEGzO\|M5RC:jPh?=
HEK293	NWHENZR1TnWwY4Tpc44h[XO\|YYm=	M1zhfVExKHWP	NX7UdHlpOjRiaILz	M1zpTHRz[W6\|YXP0bZZifGmxbjDv[kBRWEGULXfhcY1iKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{BifCBzMDD1UUBi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6	NI\XTHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi5NFA6OCd-MkS4PVAxQTB:L3G+
HEK293	M{jjOmZ2dmO2aX;uJIF{e2G7		M{m5W\|I1KGi{cx?=	MU\B[49vcXO2IHHjeIl3cXS7IHH0JI1wfXOnIGDQRXJo[W2vYTDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHPvMYV5eHKnc4Ppcochf2m2aDDHZYw1KHKncH;yeIVzKH[nY4TvdkBi\nSncjCyOEBpenNiYomg[JVidC2udXPp[oVz[XOnIILldI9zfGW{IHHzd4F6NCCHQ{WwJF0hOC5yMzFOwG0v	MkH5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NUW4PFkoRjJ2OUW1PFg6RC:jPh?=
DU145	M2nwPGN6fG:2b4jpZ4l1gSCjc4PhfS=>		MkK5OFghcHK\|	MUjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDEWVE1PSClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOTZizszNMi=>	MnP1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7OU[xOFMoRjJ2OUm2NVQ{RC:jPh?=
PC3	M3j2O2N6fG:2b4jpZ4l1gSCjc4PhfS=>		NHrXcFM1QCCqcoO=	NWPWVohNS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWEN\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB{MD6zJO69VS5?	NVXqd4NMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5PVYyPDNpPkK0PVk3OTR\|PD;hQi=>
MDA-MB-231	NYXX[ph5T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		MnPUNlQhcHK\|	NWDGPVJtT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gOU4zOyEQvF2u	M4[4XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mke4NlM3Lz5{NUK3PFI{PjxxYU6=
HEK293	M4TQemZ2dmO2aX;uJIF{e2G7			MnPFRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCxJO69VS5?	M37CblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C1Olg5Lz5{NUOwOVY5QDxxYU6=
HepG2	M{LyeGZ2dmO2aX;uJIF{e2G7		M2TaO\|IxKGi{cx?=	MULUdoFve2GldHn2ZZRqd25ib3[gVHBCWiC2cnHud4Zm[3SnZDDpckBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjCyNEBpenNiYomgVHBCWi2udXPp[oVz[XOnIHHzd4F6NCCHQ{WwJF0hOS54IN88UU4>	M3HYWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEO3N\|A1Lz5{NUSzO\|MxPDxxYU6=
HEK293	MkjvSpVv[3Srb36gZZN{[Xl?		MmjpNlQhcHK\|	NV35[JVYXHKjboPhZ5RqfmG2aX;uJI9nKFCSQWLnZY1u[SBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMhcW6ldXLheIVlKG[xcjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6	M3zvfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NESyN\|IzLz5{NUS0NlMzOjxxYU6=
HepG2	MXjGeY5kfGmxbjDhd5NigQ>?			MXfB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFfBUFQuWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO\|ZXSgbY4hcHWvYX6gTIVxTzJiY3XscJMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4xOzlizszNMi=>	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MkK4NUc,OjV2NkKyPFE9N2F-
HepG2	MV;GeY5kfGmxbjDhd5NigQ>?		M{SwTFIxKGi{cx?=	MWLB[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRvRF7BJIJqdmSrbneg[I9u[WmwIH\1d4VlKGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJIh2dWGwIFjldGczKGOnbHzzJIF{e2W\|c3XkJIF{KHKnY3XweI9zKHS{YX7zZYN1cX[jdHnvckBqdmO3YnH0[YQh\m:{IEKwJIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjB2IN88UU4>	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR7N{GzNkc,OjV2OUexN\|I9N2F-
HepaR	M3Ty[WZ2dmO2aX;uJIF{e2G7	NF:1emEyKHWP	NEj4TXEyKGSjeR?=	MYLB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCqdX3hckBJ\XCjUjDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBHSUKSMTDn[Y5mKGW6cILld5Nqd25iYYSgNUB2VSCrbnP1ZoF1\WRiZn;yJFEh\GG7IHL5JJF2[W62aYTheIl3\SCSQ2KgcYV1cG:mIILlcIF1cX[nIITvJJVvfHKnYYTl[EBkd262cn;s	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR7N{GzNkc,OjV2OUexN\|I9N2F-
HepaR	MYjGeY5kfGmxbjDhd5NigQ>?	M1rOTVEhfU1?	M3TQcVEh\GG7	NV7KcIFoSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gbJVu[W5iSHXwZXIh[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iQV7HVHRNPCCpZX7lJIV5eHKnc4Ppc44h[XRiMTD1UUBqdmO3YnH0[YQh\m:{IEGg[IF6KGK7IIH1ZY51cXSjdHn2[UBRS1JibXX0bI9lKHKnbHH0bZZmKHSxIIXueJJm[XSnZDDjc451em:u	NV\Sfmw4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PVcyOzJpPkK1OFk4OTN{PD;hQi=>
HepaR	M3zPeWZ2dmO2aX;uJIF{e2G7	M2X0WVEhfU1?	M4TBRVEh\GG7	MmTWRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhcW5iaIXtZY4hUGWyYWKgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hUE2JQ2OyJIdmdmViZYjwdoV{e2mxbjDheEAyKHWPIHnuZ5Vj[XSnZDDmc5IhOSCmYYmgZpkheXWjboTpeIF1cX[nIGDDVkBu\XSqb3SgdoVt[XSrdnWgeI8hfW62cnXheIVlKGOxboTyc4w>	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR7N{GzNkc,OjV2OUexN\|I9N2F-
C2C12	M{TjOmZ2dmO2aX;uJIF{e2G7	Ml[4N\|AhfU1?	M4jTU\|IhcHK\|	Mn;1TY5lfWO2aX;uJI9nKDJvZHXvfJkuYzOKXT3ncJVkd3OnIIXweIFs\SCrbjDtc5V{\SCFMlOxNkBk\WyuczDheEA{OCC3TTDh[pRmeiB{IHjyd{BjgSCuaYH1bYQhe2OrboTpcIxifGmxbjDjc5VvfGmwZzDhcoFtgXOrcx?=	NWT5UpI5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4N\|U2OzdpPkK1PFM2PTN5PD;hQi=>
MCF7	NFvLWYhHfW6ldHnvckBie3OjeR?=		M2j1eFE3KGi{cx?=	MmPsRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgeJJidnOoZXP0[YQhcW5iaIXtZY4hVUOINzDj[YxteyCjZoTldkAyPiCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxKD1iMD6wPFch\|ryPLh?=	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ{NkS5NEc,OjZ{Mk[0PVA9N2F-
HEK293	NX2zSIFQTnWwY4Tpc44h[XO\|YYm=	NUHidFNCOTBidV2=	MnrhNlQhcHK\|	M1zLWWFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{Bie3Onc4Pl[EBieyC{ZXPldJRweiC2cnHud4FkfGm4YYTpc44h[XRiMUCgeW0hcW6ldXLheIVlKG[xcjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN6NEK4Okc,OjZ\|OESyPFY9N2F-
3T3L1	M2TUcmZ2dmO2aX;uJIF{e2G7	Mk\BNkB2VQ>?		M3q2fXRz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|TEGgZ4VtdHNiYYPz[ZN{\WRiYYOgZYRqeG:leYTlJIRq\m[ncnXueIlifGmxbjDifUBu\WG\|dYLpcochdGmyaXSgZYNkfW23bHH0bY9vKGG2IEKgeW0h[nliT3nsJHJm\CCRIIP0ZYlvcW6pLXLhd4VlKGG\|c3H5	MlvTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUW3OFkoRjJ4NUm1O\|Q6RC:jPh?=
3T3L1	NX3pO4x7TnWwY4Tpc44h[XO\|YYm=	MnfNNkB2VQ>?		M4jkZnRz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|TEGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hTkGEUESg[ZhxemW\|c3nvckBifCB{IIXNJIJ6KHGSQ2KgcYV1cG:m	M3j0TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUm1O\|Q6Lz5{NkW5OVc1QTxxYU6=
3T3L1	MmLjSpVv[3Srb36gZZN{[Xl?	M3\xS\|IhfU1?		M1\yPHRz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|TEGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hT1SXVESg[ZhxemW\|c3nvckBifCB{IIXNJIJ6KHGSQ2KgcYV1cG:m	MoPQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUW3OFkoRjJ4NUm1O\|Q6RC:jPh?=
3T3L1	MV\GeY5kfGmxbjDhd5NigQ>?	NXfwPWFROiC3TR?=		MV3UdoFve2GldHn2ZZRqd25ib3[gVHBCWmejbX3hJIlvKG2xdYPlJFNVO0xzIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJIFlcXCxbnXjeIlvKGW6cILld5Nqd25iYYSgNkB2VSCkeTDxVGNTKG2ndHjv[C=>	M1nrW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUm1O\|Q6Lz5{NkW5OVc1QTxxYU6=
3T3L1	M4rOUWZ2dmO2aX;uJIF{e2G7	NHHJ[\|QzKHWP		M1SxO3Rz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|TEGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hVFCOIHX4dJJme3Orb36gZZQhOiC3TTDifUByWEOUIH3leIhw\A>?	NI\SWZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5OVc1QSd-Mk[1PVU4PDl:L3G+
HEK293	NXvQT3BQTnWwY4Tpc44h[XO\|YYm=	MVmxJJVO	Mn61NlQhcHK\|	NYKyPGZESWexbnnzeEBi[3Srdnn0fUBifCCSUFHSJIdidW2jIDj1cotvd3ewIH;ybYdqdilidILhcpNn\WO2ZXSgbY4hUEWNMkmzJINmdGy\|IHH0JFEhfU1iYX\0[ZIhOjRiaILzJIJ6KGS3YXytcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6	NIf0SHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm4PFMxPCd-Mk[5PFg{ODR:L3G+
C2C12	MWTGeY5kfGmxbjDhd5NigQ>?	MUWyNEB2VQ>?	MXS0JIhzew>?	MoH0WJJidnOjY4TpeoF1cW:wIH;mJHBRSVKpYX3tZUBqdiCvb4Xz[UBEOkNzMjDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBIVFWWLUSgcYVlcWG2ZXSgNk1PSkSJIIXweIFs\SCjdDCyNEB2VSCjdDC0JIhzeyCkeTDmcJVwemW\|Y3XuZ4Uh[XO\|YYm=	MlvnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMEC5PVMoRjJ5MUCwPVk{RC:jPh?=
HEK293	NYLLV3dVTnWwY4Tpc44h[XO\|YYm=		M1W3cVI1KGi{cx?=	MUHB[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRvdHHn[4VlKGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iY3jpcYVzcWNicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxKD1iMD6yOkDPxE1w	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzV7MUCwOkc,Ojd3OUGwNFY9N2F-
HepG2	NYXwTmhoTnWwY4Tpc44h[XO\|YYm=		MYqyNEBpenN?	MkHmWJJidnOjY4TpeoF1cW:wIH;mJGdCVDRvZoXz[YQhcHWvYX6gVHBCWmejbX3hJGxDTCCneIDy[ZN{\WRiaX6gbJVu[W5iSHXwS\|Ih[2WubIOgZYZ1\XJiMkCgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODRizszNMi=>	MmHiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyN{[4NlcoRjJ6MEe2PFI4RC:jPh?=
HT-29	Mm\4SpVv[3Srb36gZZN{[Xl?	M3zDXFExKHWP	MYCyOEBpenN?	Ml[2RYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhcW5iaIXtZY4hUFRvMkmgZ4VtdHNiaHHyZo9zcW6pIFHQR{BufXSjboSgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIIPp[45idGmwZzDwZZRpf2G7IHL5JI1m[XO3cnnu[{Bl\WO{ZXHz[UBqdiCFeXPsbY5FOSCuZY\lcEBifCBzMDD1UUB1emWjdHXkJIZweiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JI1mfGixZB?=	MojFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyN{[4NlcoRjJ6MEe2PFI4RC:jPh?=
COS7	M322d2Z2dmO2aX;uJIF{e2G7		NIqxR5QzPCCqcoO=	NFnXTolVemGwc3HjeIl3[XSrb36gc4YhcHWvYX6gS4FtPC2odYPl[EBRWEGUZ3HtcYEhVEKGIHX4dJJme3OnZDDpckBC\nKrY3HuJIdz\WWwIH3vcotmgSCFT2O3JINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlAxPCEQvF2u	MnTmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4MEm3NFgoRjJ6NkC5O\|A5RC:jPh?=
COS7	NEXqdGxHfW6ldHnvckBie3OjeR?=		MnHONlQhcHK\|	MV3UdoFve2GldHn2ZZRqd25ib3[gbJVu[W5iR3HsOE1nfXOnZDDQVGFT[WyyaHGgUGJFKGW6cILld5Nm\CCrbjDB[pJq[2GwIHfy[YVvKG2xbnvlfUBEV1N5IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSBzMDFOwG0v	M3G4ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NkC5O\|A5Lz5{OE[wPVcxQDxxYU6=
HEK293	NVPERVRUTnWwY4Tpc44h[XO\|YYm=		MYSxPEBpenN?	NETYXIFC\2:waYP0JIFkfGm4aYT5JIF1KEeDTESt[pV{\WRiUGDBVodidW2jIFzCSEApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZZN{\XO\|ZXSgZZMhemWlZYD0c5IhfHKjboPhZ5RqfmG2aX;uJIFnfGW{IEG4JIhzeyCkeTDkeYFtKGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFQh\|ryPLh?=	Mn3TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4Nke4O\|YoRjJ6Nk[3PFc3RC:jPh?=
Ac2F	NVniTFhrTnWwY4Tpc44h[XO\|YYm=	NILJ[4M2KHWP	Ml\vOkBpenN?	M4TDO2Fod26rc4SgZYN1cX[rdImgZZQh\nWubDDs[Y5ofGhiaIXtZY4h\myjZz30ZYdo\WRiUGDBVodidW2jMTDMRmQh\XiycnXzd4VlKGmwIILheEBC[zKIIHPlcIx{KGOxZYjwdoV{e2mwZzDQVHJGNVh\|LWTLJIF1KDVidV2gZYZ1\XJiNjDodpMh[nlib37lMYdtdyCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYm=	M4LndlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{i5NVIyLz5{OUO4PVEzOTxxYU6=
SK-N-MC	NGfXcHNyUFSVIHHzd4F6			NGracWFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1OSyClZXzsdy=>	M{XqNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
3T3-L1	NXK4eYh{TnWwY4Tpc44h[XO\|YYm=	MV2xJJVO	NX3DNmlMOTViZHH5dy=>	MU\B[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCvb4Xz[UA{XDNvTEGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hS0R\|NjDtVm5CKGW6cILld5Nqd25iYYSgNUB2VSCjZoTldkAyPSCmYYnzJIJ6KHG3YX70bZRifGm4ZTDy[YFtNXSrbXWgVGNTKG2ndHjv[C=>	NWrKUm5nRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm4O\|g4PjdpPkK5PFc5PzZ5PD;hQi=>
3T3-L1	M4[4XGZ2dmO2aX;uJIF{e2G7	NIL1[I0yKHWP	M3;3cVE2KGSjeYO=	Mn63RYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|LVyxJINmdGy\|IHHzd4V{e2WmIHHzJIlv\HWldHnvckBw\iCjZHnwc4N6fGViZHnm[oVz\W62aXH0bY9vKGG2IEGgeW0h[W[2ZYKgNVUh\GG7czDifUBQcWxiUnXkJG8he3SjaX7pcocu[mG\|ZXSgZZN{[Xl?	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTh5OEe2O{c,Ojl6N{i3Olc9N2F-
3T3-L1	MkDKSpVv[3Srb36gZZN{[Xl?	Mk\NNUB2VQ>?	Mn3MNVUh\GG7cx?=	MlvqRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|LVyxJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEOSVEHBJI1TVkFiZYjwdoV{e2mxbjDheEAyKHWPIHHmeIVzKDF3IHThfZMh[nlicYXhcpRqfGG2aY\lJJJm[WxvdHnt[UBRS1JibXX0bI9l	MkjYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl6N{i3OlcoRjJ7OEe4O\|Y4RC:jPh?=
HepG2	NEDUT4ZHfW6ldHnvckBie3OjeR?=		M4\L[VIxKGi{cx?=	MXHUdoFve2GldHn2ZZRqd25ib3[gS2FNPC22YXfn[YQhcHWvYX6gVHBCWmejbX3hJGxDTCCneIDy[ZN{\WRiaX6gbJVu[W5iSHXwS\|Ih[2WubIOgZYZ1\XJiMkCgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODN7IN88UU4>	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODF7OUK1N{c,OzBzOUmyOVM9N2F-
3T3L1	MmfzSpVv[3Srb36gZZN{[Xl?	MmjwOUB2VQ>?		NIKz[YZKdmS3Y4Tpc44hd2ZibHnwbYQh[WOldX31cIF1cW:wIHnuJI1wfXOnIEPUN2wyKGOnbHzzJIF1KDVidV2gZpkhV2muIGLl[EBQKHO2YXnubY5oNWKjc3XkJIF{e2G7	M{XQVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMUm5NlU{Lz5\|MEG5PVI2OzxxYU6=
3T3L1	NGX3[HVHfW6ldHnvckBie3OjeR?=	NW\oOpJ4OiC3TR?=		MmrYTY5lfWO2aX;uJI9nKGyrcHnkJIFk[3WvdXzheIlwdiCrbjDtc5V{\SB\|VEPMNUBk\WyuczDheEAzKHWPIHL5JG9qdCCUZXSgU{B{fGGrbnnu[{1j[XOnZDDhd5NigQ>?	MkPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzOUmyOVMoRjNyMUm5NlU{RC:jPh?=
HepG2	NFu4UGdHfW6ldHnvckBie3OjeR?=	NWnib20xRjFyMDDuUS=>	NEm2U3YzOCCqcoO=	NVS3[ZY{XHKjboPhZ5RqfmG2aX;uJI9nKEeDTESteIFo\2WmIHj1cYFvKFCSQWLnZY1u[SCOQlSg[ZhxemW\|c3XkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGG2IE6xNFAhdk1iYX\0[ZIhOjBiaILzJIlvKHC{ZYPlcoNmKG:oIEWgeW0hWFCDUnfhcY1iKGGwdHHnc45qe3RiR2e5OlYzKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODF7OUK1N{c,OzBzOUmyOVM9N2F-
COS7	MkLGSpVv[3Srb36gZZN{[Xl?		MoXGNlQhcHK\|	NVWyS4E4SWexbnnzeEBi[3Srdnn0fUBifCC{ZXPvcYJqdmGwdDDoeY1idiCJQVy0MWRDTCCodYPl[EBRWEGUZ3HtcYEhVEKGIHX4dJJme3OnZDDpckBEV1N5IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkO5OkDPxE1w	NETKVGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEKyNFYxOid-M{CyNlA3ODJ:L3G+
COS7	NIOwcWFHfW6ldHnvckBie3OjeR?=		M{jGXVI1KGi{cx?=	NIXJb5pC\2:waYP0JIFkfGm4aYT5JIF1KHKnY3;tZolv[W62IHj1cYFvKEeDTEStSGJFKG[3c3XkJHBRSVKpYX3tZUBNSkRiZYjwdoV{e2WmIHnuJGNQWzdiY3XscJMh[W[2ZYKgNlQhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwM{m2JO69VS5?	NXLiT4NnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyOFE6ODdpPkOwNlQyQTB5PD;hQi=>
HEK293	M2Tn[mZ2dmO2aX;uJIF{e2G7		MY[xPEBpenN?	NI\PXplC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGdCVDRiZoXz[YQhWFCDUnfhcY1iNUyERDDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHHmeIVzKDF6IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlEzKM7:TT6=	MoHWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{OU[2PFUoRjNyMkm2Olg2RC:jPh?=
HEL 293H	Mn3FSpVv[3Srb36gZZN{[Xl?		NIiwVWkyPiCqcoO=	NY\0[GlRXHKjboPhZ5RqfmG2aX;uJI9nKEeDTEStSGJFKG[3c3XkJIh2dWGwIGDQRXJo[W2vYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIGXBV{1jdGFiSFXMJFI6O0hiY3XscJMheHKnaX7jeYJifGWmIH\vdkAyPiCqcoOg[o9tdG:5ZXSgZpkhTlKHVDDzeYJ{fHKjdHWgZYRlcXSrb36gZY5lKG2nYYP1doVlKGGodHXyJFIhcHK\|IHL5JHRTNU[URWSgZZN{[XluIFXDOVAhRSByLkCwPEDPxE1w	MnjMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB2MkmwPVcoRjNyNEK5NFk4RC:jPh?=
HEK293	M2DOcWZ2dmO2aX;uJIF{e2G7		NHK3XIYyQCCqcoO=	NWHKSnNKXHKjboPhZ5RqfmG2aX;uJI9nKGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIlv[3WkYYTl[EBnd3JiMUigbJJ{KGK7IHT1ZYwhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxKD1iMD6wN\|kh\|ryPLh?=	M3:0TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=
CV-1	MkXlSpVv[3Srb36gZZN{[Xl?			M2C5VXRme3SnZDDmc5IhfHKjboPhZ5RqfmG2aX;uJI9nKGi3bXHuJHBRSVJiZ3HtcYEuT2GuNDDjbIlu\XKjIHnuJGNXNTFiY3XscJMtKEWFNUCgQUAxNjB3IN88UU4>	NG\KXno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjFxJ{7DbGVOSkx:L3G+
3T3L1	M{j4V2Z2dmO2aX;uJIF{e2G7		MUG3JIRigXN?	M4jZNXVxemWpdXzheIlwdiCxZjDGRXMheHKxdHXpckBmgHC{ZYPzbY9vKGmwIHTp[oZmemWwdHnheIVlKG2xdYPlJFNVO0xzIHPlcIx{KGGodHXyJFch\GG7czDifUB4\XO2ZYLuJIJtd3RiYX7hcJl{cXN?	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlEwLz6FaFXNRmw9N2F-
3T3L1	M{njeWZ2dmO2aX;uJIF{e2G7		NFXBUlI4KGSjeYO=	MnHyWZBz\We3bHH0bY9vKG:oIHHQNkBxem:2ZXnuJIV5eHKnc4Ppc44hcW5iZHnm[oVz\W62aXH0[YQhdW:3c3WgN3Q{VDFiY3XscJMh[W[2ZYKgO{Bl[Xm\|IHL5JJdme3Sncn6gZoxwfCCjbnHsfZNqew>?	NGLVbIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjFxJ{7DbGVOSkx:L3G+
3T3L1	MorNSpVv[3Srb36gZZN{[Xl?		NWfreo95OjRiaILz	NW\GUHJ4UW6qaXLpeIlwdiCxZjDjbJJwdmmlIHnud5VtcW5vc4TpcZVt[XSnZDDpcpN2dGmwIILld4l{fGGwY3WgbY4h\GmoZnXy[Y51cWG2ZXSgcY92e2ViM2SzUFEh[2WubIOgZZN{\XO\|ZXSgZZMhOi2mZX;4fU1FNVt\|SG2t[4x2[2:\|ZTD1dJRic2ViYX\0[ZIhOjRiaILzJIJ6KHOlaX70bYxt[XSrb36gZ492dnSrbnegZY5idHm\|aYO=	M13iZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=
3T3L1	NEOzSVZHfW6ldHnvckBie3OjeR?=	MoTZNVAhfU1?	M3HOcFI1KGi{cx?=	MVzJcoR2[3Srb36gc4YhcW6\|dXzpck1{fGmvdXzheIVlKDJvZHXvfJkuTC2dM1jdMYdtfWOxc3WgeZB1[WunIHnuJIRq\m[ncnXueIlifGWmIH3veZNmKDOWM1yxJINmdGy\|IHH0JFExKHWPIHHmeIVzKDJ2IHjyd{BjgSC\|Y3nueIltdGG2aX;uJINwfW62aX7nJIFv[Wy7c3nz	NV\hVYRpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJzLze+R4hGVUKOPD;hQi=>
3T3L1	M3jXZ2Z2dmO2aX;uJIF{e2G7	MViyJJVO	NHjzTWYyPCCmYYnz	MkPDRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUIHnuJI1wfXOnIEPUN2wyKGOnbHzzJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIHHkbZBw[3m2ZTDkbYZn\XKnboTpZZRqd25iYYSgNkB2VSCjZoTldkAyPCCmYYnzJIJ6KE:rbDDS[YQhVyC\|dHHpcolv\yCvZYToc4Q>	NYjXPHE1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJzLze+R4hGVUKOPD;hQi=>
U2OS	MmnvSpVv[3Srb36gZZN{[Xl?	NHr4V40yOCC3TR?=	MYeyOEBpenN?	MkT6RYN1cX[jdHnvckBw\iCSUFHSS{ApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCqdX3hckBWOk:VIHPlcIx{KGG2IEGwJJVOKGmwY4XiZZRm\CCob4KgNlQhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigQ>?	NI\0dFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjFxJ{7DbGVOSkx:L3G+

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Cyclin D1 / CDK2 / CDK4 / Bax / Bcl-2 ; PubMed: 29725405 Oncol Lett A431 cells at 80% confluence were stimulated with rosiglitazone for 24 h, and the effects on Bax, Bcl-2, cyclin D1, cdk2 and cdk4 expression were analyzed by western blotting. Data were quantified by scanning densitometry, and the ratio of the protein of interest was normalized to GAPDH. Data are presented as means ± SEM (n=3). #P<0.05 (10, 20, 30, 40 mM rosiglitazone treatment compared with control treatment for cyclin D1/GAPDH and Cdk4/GAPDH; 30, 40 mM rosiglitazone treatment compared with control treatment for Cdk2/GAPDH) as analyzed with one-way ANOVA.	29725405
Immunofluorescence	PPARγ / E-cadherin ; PubMed: 27145370 Oncotarget Increased PPARγ (green) and E-cadherin (red) with rosiglitazone treatment and overlaid image showing colocalization (yellow, arrows) in high glucose condition (30 mM).	27145370
Growth inhibition assay	Cell viability ; PubMed: 29725405 Oncol Lett MTS assay of A431 cell viability. A431 cells were incubated with different concentrations (0–100 µM) rosiglitazone for 24 h. Cell viability was determined by MTS assay kit. Data are presented as the mean ± standard deviation of three independent experiments. #P<0.05 (0 µM rosiglitazone vs. 100 µM rosiglitazone) as analyzed with one-way analysis of variance.	29725405

In vivo

Rosiglitazone administration results in significant bone loss, including a decrease in bone volume, trabecular width, and trabecular number and an increase in trabecular spacing. Rosiglitazone also leads to a decrease in bone formation rate, with a simultaneous increase in fat content in the bone marrow. Rosiglitazone decreases the expression of the osteoblast-specific genes Runx2/Cbfa1, Dlx5, and alpha1(I)collagen, whereas the expression of the adipocyte-specific fatty acid binding protein aP2 is increased. ^[2] Rosiglitazone upregulates gene transcripts encoding mitochondrial proteins in white adipocytes from ob/ob mice accompanied by an increase in mitochondrial mass and changes in mitochondrial structure. ^[4]

Protocol

References

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Solubility (25°C)

In vitro	DMSO	71 mg/mL (198.64 mM)
	Water	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 4% DMSO+30% PEG 300+5% Tween 80+ddH2O For best results, use promptly after mixing.	5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Rosiglitazone (BRL 49653) SDF

Molecular Weight	357.43
Formula	C₁₈H₁₉N₃O₃S
CAS No.	122320-73-4
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	CN(CCOC1=CC=C(C=C1)CC2C(=O)NC(=O)S2)C3=CC=CC=N3

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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The Serial Dilution Calculator Equation

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Computed Result

C1=C0/X	C1:	LOG(C1):
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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-05-17)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT01100619	Completed	Drug: rosiglitazone\|Drug: XL184	Papillary Thyroid Cancer\|Follicular Thyroid Cancer\|Huerthle Cell Thyroid Cancer\|Renal Cell Carcinoma	Exelixis	April 2010	Phase 1
NCT00609362	Completed	Drug: Rosiglitazone\|Drug: Placebo pill	Change in Bone Mineral Density\|Change in Bone Marrow Fat Content	University of Aarhus	January 2008	Phase 2
NCT00405015	Completed	Drug: rosiglitazone	Ischemia-Reperfusion Injury\|The Metabolic Syndrome	Radboud University\|GlaxoSmithKline	April 2007	Phase 2
NCT00486187	Completed	Drug: rosiglitazone\|Drug: metformin or sulfonylurea	Type 2 Diabetes Mellitus	Dr. Milan Gupta\|GlaxoSmithKline\|Canadian Collaborative Research Network	April 2006	Not Applicable
NCT00370305	Completed	Drug: rosiglitazone	Metabolic Syndrome\|Impaired Glucose Tolerance	Charite University Berlin Germany	May 2004	Phase 2

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Rosiglitazone (BRL 49653)