Rosiglitazone (BRL 49653)

Name: Rosiglitazone (BRL 49653)
Brand: Selleck Chemicals
SKU: S2556
Rating: 5 (39 reviews)

For research use only.

Catalog No.S2556

39 publications

Rosiglitazone (BRL 49653) Chemical Structure

CAS No. 122320-73-4

Rosiglitazone (BRL 49653) is a potent antihyperglycemic agent and a potent thiazolidinedione insulin sensitizer with IC50 of 12, 4 and 9 nM for rat, 3T3-L1 and human adipocytes, respectively. Rosiglitazone is a pure ligand of PPAR-gamma, and has no PPAR-alpha-binding action. Rosiglitazone modulates TRP channels and induces autophagy. Rosiglitazone prevents ferroptosis.

Selleck's Rosiglitazone (BRL 49653) has been cited by 39 publications

Nat Med, 2018, 24(3):360-367

PubMed: 29377004 ( click the link to review the publication )

Antioxidants (Basel), 2021, 10(2)155

PubMed: 33494520 ( click the link to review the publication )

Int J Mol Sci, 2021, 22(11)5559

PubMed: 34074054 ( click the link to review the publication )

Front Cardiovasc Med, 2021, 8:698056

PubMed: 34336956 ( click the link to review the publication )

Hum Cell, 2021, 10.1007/s13577-021-00579-z

PubMed: 34304386 ( click the link to review the publication )

Nat Commun, 2020, 11(1):71

PubMed: 31900415 ( click the link to review the publication )

Cell Death Dis, 2020, 11(9):752

PubMed: 32929062 ( click the link to review the publication )

FASEB J, 2020, 10.1096/fj.202001758R

PubMed: 33070393 ( click the link to review the publication )

Cancer Cell Int, 2020, 19;20:58

PubMed: 32099531 ( click the link to review the publication )

Exp Cell Res, 2020, S0014-4827(20)30690-X

PubMed: 33385414 ( click the link to review the publication )

Cancer Chemother Pharmacol, 2020, 85(2):273-284

PubMed: 31907647 ( click the link to review the publication )

Hum Cell, 2020, 10.1007/s13577-020-00420-z

PubMed: 32870449 ( click the link to review the publication )

Hum Cell, 2020, 10.1007/s13577-020-00425-8

PubMed: 32886306 ( click the link to review the publication )

Bioengineered, 2020, 11(1):1350-1367

PubMed: 33289586 ( click the link to review the publication )

Mol Omics, 2020, 16(6):533-542

PubMed: 33315023 ( click the link to review the publication )

Cell Death Differ, 2019, 10.1038/s41418-019-0299-4

PubMed: 30737476 ( click the link to review the publication )

Neuroimage, 2019, 10.1016/j.neuroimage.2019.05.069

PubMed: 31158477 ( click the link to review the publication )

J Cell Mol Med, 2019, 23(11):7406-7416

PubMed: 31475784 ( click the link to review the publication )

Mediators Inflamm, 2019, 2019:1769374

PubMed: 31772499 ( click the link to review the publication )

BMC Cancer, 2019, 19(1):204

PubMed: 30845932 ( click the link to review the publication )

Exp Biol Med (Maywood), 2019, 244(3):252-261

PubMed: 30714402 ( click the link to review the publication )

Ann Transl Med, 2018, 6(8):152

PubMed: 29862241 ( click the link to review the publication )

Toxicol Appl Pharmacol, 2018, 355:112-126

PubMed: 29782964 ( click the link to review the publication )

Theranostics, 2018, 8(15):4262-4278

PubMed: 30128052 ( click the link to review the publication )
Aging Cell, 2018, 10.1111/acel.12774

PubMed: 29740932 ( click the link to review the publication )

Cancer Sci, 2018, 109(7):2243-2255

PubMed: 29791764 ( click the link to review the publication )

PPAR Res, 2018, 2018:8512745

PubMed: 29686698 ( click the link to review the publication )

Int Immunopharmacol, 2018, 56:90-97

PubMed: 29367091 ( click the link to review the publication )

Process Biochemistry, 2018, 10.1016/j.procbio.2018.01.008

PubMed: ( click the link to review the publication )

Med Sci Monit, 2018, 24:6200-6207

PubMed: 30185768 ( click the link to review the publication )

Clin Exp Immunol, 2017, 189(2):211-225

PubMed: 28369855 ( click the link to review the publication )

Reprod Sci, 2017, 24(12):1590-1599

PubMed: 28322133 ( click the link to review the publication )

Oncol Rep, 2017, 38(5):3220-3226

PubMed: 28901466 ( click the link to review the publication )

Oncotarget, 2017, 8(56):95914-95930

PubMed: 29221176 ( click the link to review the publication )

J Immunol, 2016, 196(3):1044-59

PubMed: 26740111 ( click the link to review the publication )

RSC Advances, 2016, 10.1039/c6ra05637g

PubMed: ( click the link to review the publication )

Toxicol Appl Pharmacol, 2015, 285(1):51-60

PubMed: 25841593 ( click the link to review the publication )

J Leukoc Biol, 2014, 95(4):587-98

PubMed: 24338629 ( click the link to review the publication )

Stroke, 2013, 44(12):3498-508

PubMed: 24135932 ( click the link to review the publication )

Purity & Quality Control

Choose Selective PPAR Inhibitors

Biological Activity

Description

Targets

PPAR ^[1]
(Cell-free assay)

TRPC ^[5]
()

Ferroptosis ^[6]
()

In vitro

Rosiglitazone markedly increase phosphorylation of threonine 172 within the α subunit of AMPK, with increased AMP:ATP ratio. Rosiglitazone has been reported to decrease cholesterol synthesis in a number of cell lines in a peroxisome proliferator-activated receptor γ-independent manner. Rosiglitazone activates both α1- and α2-containing AMPK complexes, and this leads to a marked increase in the phosphorylation of acetyl-CoA carboxylase. ^[1] Rosiglitazone activates PPAR-gamma2 which acts as a dominant inhibitor of osteoblastogenesis in murine bone marrow in vitro.^[2] Rosiglitazone increases adiponectin secretion from omental cells up to 2.3-fold higher, whereas secretion from subcutaneous adipose cells is unaffected. ^[3] Rosiglitazone changes the morphological features and protein profiles of mitochondria in 3T3-L1 adipocytes. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
HepG2	NFLZ[VVHfW6ldHnvckBie3OjeR?=			M2nySGVn\mWldHn2[UBkd26lZX70doF1cW:wIHHnZYlve3RiaIXtZY4hWFCDUnfhcY1iKGW6cILld5Nm\CCrbjDI[ZBIOiClZXzsd{whTUN3ME2wMlAzKM7:TR?=	NHjRPGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUiwNVgyPyd-MUW4NFE5OTd:L3G+
CV-1	MmDVSpVv[3Srb36gZZN{[Xl?			M2HoZWlvKH[rdILvJJRz[W6\|Y4LpdJRqd26jbDDhZ5RqfmG2aX;uJI9nKFCncn;4bZNwdWVicILvcIln\XKjdH;yJIFkfGm4YYTl[EBz\WOncITvdkBo[W2vYTCoVHBCWiliZYjwdoV{e2WmIHnuJGNXNTFiY3XscJMtKEWFNUCgQUAxNjB4IN88UU4>	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85PTd4OUC3K\|45PTd4OUC3QE9iRg>?
CV-1	MXrGeY5kfGmxbjDhd5NigQ>?			M{LITmFkfGm4YYTpc44hd2ZicHXyc5hqe2:vZTDwdo9tcW[ncnH0c5Ih[WO2aY\heIVlKHKnY3XweI9zKGejbX3hJI1m[XO3cnXkJIJ6KGmwZIXjeIlwdiCxZjC1NEUhd2ZibXH4bY12dSCjbHvhcIlv\SCyaH;zdIhifGG\|ZTDhZ5Rqfmm2eTygeJJidnOoZXP0bY9vKGG\|c3H5JIlvKEOYLUGgZ4VtdHNuIFXDOVAhRSByLkC4PVE{KM7:TT6=	M4LGRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl6M{[2NlAoRjl6M{[2NlA9N2F-
3T3-L1	NWX4XZlmTnWwY4Tpc44h[XO\|YYm=			NX;PN\|BtW3SrbYXsZZRqd25ib3[gZYRqeG:pZX7ld4l{KGmwIEPUN{1NOSClZXzsd{BqeyCneIDy[ZN{\WRiYYOgZ49v[2WwdILheIlwdiCncYXpeoFt\W62IITvJJRp\SCdIEGtNVREZSC3cIThb4Uh[2:3boTzJIFnfGW{IITy[YF1dWWwdDD3bZRpKDBwMjD1[{9uVCC2cn;ncIl1[XqxbnWsJGVEKD1iMD6wPUDPxE1w	NYCze\|c6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC5OVYzOTNpPkGwPVU3OjF\|PD;hQi=>
CV-1	NXHJUm1QTnWwY4Tpc44h[XO\|YYm=			NEnkTXRO[XirbXHsJJJmeG:{dHXyJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUHXyc5hqe2:vZTDwdo9tcW[ncnH0c5Ih[WO2aY\heIVlKHKnY3XweI9zKGejbX3hJGdidDRiY3jpcYVzcWNiaX6geJJidnOrZX70cJkhfHKjboPm[YN1\WRiQ2[tNUBk\WyuczDifUBnfW6ldHnvcoFtKGG\|c3H5MkwhTUN3MDC9JFAvODR\|IN88UU4>	M13pflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{KwPFU1Lz5zMUeyNFg2PDxxYU6=
COS1	NF\pZ5dHfW6ldHnvckBie3OjeR?=			MnLiRYdwdmm\|dHnjJIFkfGm4aYT5JJdieyCmZYTldo1qdmWmIHnuJGNQWzFiY3XscJMhfHKjboPm[YN1\WRid3n0bEBISUxiND3QVGFTKGejbX3hJJJm[2WydH;yMEBGSzVyIE2gNE4xOjFizszNMi=>	M4PXfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{NkG3PVI1Lz5zMk[xO\|kzPDxxYU6=
COS1	NGCzXY1HfW6ldHnvckBie3OjeR?=			M1zDZWlvKH[rdILvJIFod26rc4SgZYN1cX[rdImgeIV{fGWmIH\vdkB1emGwc3HjeIl3[XSrb36gbY4hcHWvYX6gVHBCWiCpYX3tZU1I[Wx2IHPobY1memmlIFPPV{0yKGOnbHzzMEBGSzVyIE2gNE4xOiEQvF2u	M3Pu[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{N{K5OlY5Lz5zMkeyPVY3QDxxYU6=
COS1	NHq3T4VHfW6ldHnvckBie3OjeR?=			NFHOR3FKdiC4aYTyc{B1emGwc3HjeIl3[XSrb36gc4YhcHWvYX6gdIVzd3irc3;t[UBxem:uaX\ldoF1d3JiYXP0bZZifGWmIILlZ4VxfG:{IHfhcY1iKG2nYYP1doVlKGmwIGDQRXIuT0GONDDjbIlu\XKrYzDDU3MuOSClZXzsd{whTUN3MDC9JFAvODJizszNMi=>	M2PSZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OEezOVE4Lz5zMki3N\|UyPzxxYU6=
CV-1	M4D3N2Z2dmO2aX;uJIF{e2G7			M{f3b2lvKH[rdILvJIV3[Wy3YYTpc44h[WejaX7zeEBTYFJvYXzwbIEwWFCDUj3nZY1u[SCrbjDDWk0yKGOnbHzzJIJ6KGOxdILhcpNn\WO2aX;uJIF{e2G7IIfhd{Bl\XSncn3pcoVlNCCHQ{WwJF0hOC5\|MkWg{txONg>?	M4XnbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OUW0NFYyLz5zMkm1OFA3OTxxYU6=
3T3-L1	NGq1SVZHfW6ldHnvckBie3OjeR?=			MVrF[oZm[3SrdnWgZ49v[2WwdILheIlwdiCob4Kg[Y5p[W6lZX3lcpQhd2ZiaX7zeYxqdi2rbnT1Z4VlKHS{aXfsfYNmemmmZTDhZ4N2dXWuYYTpc44hcW5iM2SzMWwyKGOnbHzzMEBGSzNyIE2gNE4xOiEQvF2u	MnHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR3MEW2OFQoRjF2NUC1OlQ1RC:jPh?=
HepG2	NH7kV\|NHfW6ldHnvckBie3OjeR?=			NWL1c\|ZsTW[oZXP0bZZmKGOxbnPlcpRz[XSrb36gZYdicW6\|dDDoeY1idiCSUFHS[4FudWFiZYjwdoV{e2WmIHnuJGhmeEd{IHPlcIx{NCCHQ{WwJF0hOC5yM{mg{txONg>?	NF7pXI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkGwO\|E2OCd-MU[xNFcyPTB:L3G+
U2OS	M{\DNmZ2dmO2aX;uJIF{e2G7			M1jMVGVn\mWldDDvckBRWEGUZ3HtcYEhfHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JIlvKFV{T2OgZ4VtdHNuIFXDOVAhRSByLkCzJO69VS5?	M2\tflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4M{CwPVQ1Lz5zNkOwNFk1PDxxYU6=
Huh7	MYXGeY5kfGmxbjDhd5NigQ>?			NYW1TmRwTnWwY4Tpc45idCCjY4Tpeol1gSCjdDDoeY1idiCSUFHSJIdidW2jIHnuJGh2cDdiY3XscJMh[nlidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gNE4zOiEQvF2u	MlP0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ\|Nk[2NFEoRjF4M{[2OlAyRC:jPh?=
Huh7	Mm\vSpVv[3Srb36gZZN{[Xl?			MoP4SYZn\WO2IH;uJHBRSVJiZ3HtcYEhfHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JIlvKEi3aEegZ4VtdHNuIFXDOVAhRSByLkKyJO69VS5?	NXO3N41URGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0OVExQDdpPkG2OFUyODh5PD;hQi=>
HEK293	MWDGeY5kfGmxbjDhd5NigQ>?			NXrZTol4SWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhemWlZYD0c5IhcW62ZYLhZ5Rqd25id3n0bEB{fGW{b3nkJJJm[2WydH;yJINw[WO2aY\heI9zNTFiYomgSXlHWCCkYYPl[EBz\XCxcoTldkBo\W6nIHHzd4F6	M{[ydFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
HEK293	MWXGeY5kfGmxbjDhd5NigQ>?			M{fhPWFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XO\|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJJJm[2WydH;yJIlvfGW{YXP0bY9vKHerdHigdoV1cW6xaXSgXE1z\WOncITvdkBidHCqYTDifUBGYU[SIHLhd4VlKHKncH;yeIVzKGenbnWgZZN{[Xl?	NEnyeI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNk[4NFE2QSd-MU[2PFAyPTl:L3G+
CV1	NHTEZWJHfW6ldHnvckBie3OjeR?=			NH73ToVVemGwc3HjeIl3[XSrb36gc4YhWFCDUnfhcY1iKGmwIFPWNUBk\WyuczygSWM2OCB;IECuNFc3KM7:TT6=	NGraTJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkiyNVc3QSd-MU[4NlE4Pjl:L3G+
NIH3T3	M1PRXmZ2dmO2aX;uJIF{e2G7			NXzxTI5CSWO2aY\peJkh[XRiaIXtZY4hWFCDUjDnZY1u[SC2cnHud4Zm[3SnZDDpckBPUUh\|VEOgZ4VtdHNiYomgcJVkcW[ncnHz[UBi[3Srdnn0fUBie3OjeTygSWM2OCB;IECuN\|Ih\|ryPLh?=	MkTqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ6NUSwPFUoRjF4OEW0NFg2RC:jPh?=
CV1	NH20d\|hHfW6ldHnvckBie3OjeR?=			NIrVb2hVemGwc3HjeIl3[XSrb36gc4YhcHWvYX6gVHBCWmejbX3hJIlvKEOYMTDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjNyODFOwG0v	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl5MEO5NUc,OTZ7N{CzPVE9N2F-
HepG2	MkTWSpVv[3Srb36gZZN{[Xl?			M4PyeGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWiCpYX3tZUBqdiCjIFjldGczKGOnbHzzJIJ6KFCSQWKtS2FNPCC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlE2QCEQvF2u	MnTQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7N{OzOVgoRjF4OUezN\|U5RC:jPh?=
HepG2	MWrGeY5kfGmxbjDhd5NigQ>?			Moq3RYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFTKGejbX3hJIlvKEincFeyJINmdGy\|IHL5JHBRSVJvR1HMOEB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkG1PEDPxE1w	M1L1bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUe5N\|QyLz5zNkm3PVM1OTxxYU6=
CV1	MYDGeY5kfGmxbjDhd5NigQ>?			Ml\TRYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIlvKEOYMTDj[YxteyxiRVO1NEA:KDBwM{C4JO69VS5?	M{nqTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MEC1N\|k1Lz5zN{CwOVM6PDxxYU6=
Huh7	MnHpSpVv[3Srb36gZZN{[Xl?			MnzBRYN1cX[rdImgZZQhcHWvYX6gVHBCWiCpYX3tZUBqdiCKdXi3JINmdGy\|IHL5JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMkKg{txONg>?	NV\sdmdoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUewN\|QyPDlpPkG3NFM1OTR7PD;hQi=>
CV1	NWrUd3g2TnWwY4Tpc44h[XO\|YYm=			M1O4WGFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIFPWNUBk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkOwPEDPxE1w	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF{OUeyOUc,OTdzMkm3NlU9N2F-
HepG2	M4nsd2Z2dmO2aX;uJIF{e2G7			MmDnRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgbY4hUGWyR{KgZ4VtdHNiYomgVHBCWi2JQVy0JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEiyJO69VS5?	MoC0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzNUewNVkoRjF5MUW3NFE6RC:jPh?=
HEK293	Mmj2SpVv[3Srb36gZZN{[Xl?			MmiyRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{BjgSCJQVy0JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEO1JO69VS5?	NIXsTok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{K5NlYxPid-MUeyPVI3ODZ:L3G+
HEK293	M1HhVWZ2dmO2aX;uJIF{e2G7			MWTBZ5Rqfmm2eTDheEBpfW2jbjDh[Ilxd3OnIITpd5N2\SCSUFHSJIdidW2jIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYomgVHBCWi2JQVy0JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwM{Gg{txONg>?	MnvqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd\|NEOzO\|EoRjF5M{SzN\|cyRC:jPh?=
L6	NWTvXW1PTnWwY4Tpc44h[XO\|YYm=			Mlu3SYZn\WO2IH;uJIZifHS7IHHjbYQhd3irZHH0bY9vKGmwIILheEBNPiClZXzsd{whTUN3MDC9JFUh\|ryPLh?=	NVjRUmVvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezOFM{PzFpPkG3N\|Q{OzdzPD;hQi=>
HepG2/C3A	MXPGeY5kfGmxbjDhd5NigQ>?			NYLvdndtSWO2aY\heIlwdiCxZjDoeY1idiCSUFHS[4FudWFibHnnZY5lKGKrbnTpcoch\G:vYXnuMY1m\GmjdHXkJJRz[W6\|Y4LpdJRqd26jbDDhZ5Rqfmm2eTDpckBpfW2jbjDI[ZBIOi:FM1GgZ4VtdHNiY3:teJJidnOoZXP0[YQhf2m2aDDmeZNm\CCJYXy0MWxDTCCkeTDjc5Rz[W6\|ZnXjeIlwdiCjc4PhfUwhTUN3MDC9JFAvODB\|ODFOwG0v	MnvVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MEO2PFgoRjF5NECzOlg5RC:jPh?=
CV1	NH\ve\|lHfW6ldHnvckBie3OjeR?=		NVz0PIJHOjRiaILz	M2XtVGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIlvKEOYMTDj[YxteyCkeTDHRWw1KHS{YX7zZYN1cX[jdHnvckBie3OjeTDh[pRmeiB{NDDodpMtKEWFNUCgQUAxNjB\|IN88UU4>	NXfoWWkxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NFczOjVpPkG3OVA4OjJ3PD;hQi=>
CV1	MlXkSpVv[3Srb36gZZN{[Xl?		MYKyOEBpenN?	Mk\kRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT[WyyaHGgbY4hS1ZzIHPlcIx{KGK7IFfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7IHHmeIVzKDJ2IHjyd{whTUN3MDC9JFMvPDZizszNMi=>	NFrR[II9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WwO\|IzPSd-MUe1NFczOjV:L3G+
NIH3T3	Ml;aSpVv[3Srb36gZZN{[Xl?			MVTB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO\|ZXSgbY4hVkmKM2SzJINmdGy\|IHL5JGdCVDRidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gNE4{OiEQvF2u	NEW5VlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[yOFc4Pyd-MUe2NlQ4Pzd:L3G+
HEK293	MVzGeY5kfGmxbjDhd5NigQ>?			MYjB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W\|c3XkJIF{KGGSMjDn[Y5mKGmwZIXjeIlwdixiRVO1NEA:KDBwMUKg{txONg>?	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB{OUG3Okc,OThyMkmxO\|Y9N2F-
HEK293	MUPGeY5kfGmxbjDhd5NigQ>?			NVP3UlRLSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCkeTDHRWw1KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNVkh\|ryPLh?=	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB{OUG3Okc,OThyMkmxO\|Y9N2F-
CV1	MXvGeY5kfGmxbjDhd5NigQ>?			MlTNRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKEOYMTDj[YxteyCkeTDy[YNmeHSxcjD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjNyODFOwG0v	M37WeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MEK5NVc5Lz5zOECyPVE4QDxxYU6=
HEK293	MYTGeY5kfGmxbjDhd5NigQ>?			MWjB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCKRVuyPVMh[2WubIOgZpkhT0GONDD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjB2NTFOwG0v	NIfOWGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK5NVY1PSd-MUiyPVE3PDV:L3G+
U2OS	NEHWbmFHfW6ldHnvckBie3OjeR?=			NYriUo5oSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEhcW5iVULPV{Bk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkCyJO69VS5?	MonmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|Mkm3OVEoRjF6M{K5O\|UyRC:jPh?=
HepG2	NHrzOWFHfW6ldHnvckBie3OjeR?=			NYHOUY91SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjldGczKGOnbHzzJIJ6KEeDTESgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5{MkOg{txONg>?	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN7NEmwO{c,OTh\|OUS5NFc9N2F-
HepG2	M1[wWWZ2dmO2aX;uJIF{e2G7			Ml3uRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIHj1cYFvKEincFeyJINmdGy\|IHHzd4V{e2WmIHHzJIlv\HWldHnvckBw\iC{ZXPldJRweiC2cnHud4FkfGm4YYTpc44h[nlicnXwc5J1\XJiZ3Xu[UBie3OjeTDy[YxifGm4ZTD0c{Bkd262cn;sMEBGSzVyIE2gNE4xPSEQvF2u	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ{NUW1PUc,OTh4MkW1OVk9N2F-
RAW264.7	NVq1dIN1TnWwY4Tpc44h[XO\|YYm=			MkS0V5VxeHKnc4Ppc44hd2ZiTGDTM2lHVi2pYX3tZU1{fGmvdXzheIVlKE6RIIDyc4R2[3Srb36gbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNuIFnDOVAhRSBzNz61JO69VS5?	M2XrRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OEC5N\|I2Lz5zOEiwPVMzPTxxYU6=
CV1	NX\pWZpFTnWwY4Tpc44h[XO\|YYm=			M4fZbmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS1ZzIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiZoXz[YQhT2GuND3ERmQh[nlidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gNE4xOzNizszNMi=>	NIjofW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEiyOlIxPSd-MUi4NlYzODV:L3G+
CV1	MmGzSpVv[3Srb36gZZN{[Xl?			NH;MdY9C\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKjbIDoZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJIFnemmlYX6g[5Jm\W5ibX;ub4V6KEOYMTDj[YxteyClbz30doFve2[nY4Tl[EB4cXSqIH\1d4VlKEejbEStSGJFKGK7IITyZY5{[WO2aY\heIlwdiCjc4PhfUwhTUN3MDC9JFMvPDZizszNMi=>	M4npclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OEK2NlA2Lz5zOEiyOlIxPTxxYU6=
HepG2	MV;GeY5kfGmxbjDhd5NigQ>?			M3Xu[WFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmGucHjhJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gbJVu[W5iSHXwS\|Ih[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBI[Wx2IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMEO5JO69VS5?	M2nHTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OEO1O\|E6Lz5zOEizOVcyQTxxYU6=
HepG2	NHWyVFJHfW6ldHnvckBie3OjeR?=			Mof3RYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDoeY1idiCKZYCgS\|Ih[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBI[Wx2LVTCSEBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlA1KM7:TT6=	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{e3Okc,OTlyNUO3O\|Y9N2F-
HeLa	MWrGeY5kfGmxbjDhd5NigQ>?			NYjCPIhmSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFibHnnZY5lKGKrbnTpcoch\G:vYXnuJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\UyjIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiR3HsOE1FSkRiYYPz[ZN{\WRiYYOgeJJidnOlcnnweIlwdmGuIHHjeIl3[XSrb36gZpkhT2GuNDDy[ZNxd26\|ZTDlcIVu\W62LXTybZZmdiCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlAyPSEQvF2u	M3nMTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7Mke1PVY{Lz5zOUK3OVk3OzxxYU6=
BL21	NIfjUVlHfW6ldHnvckBie3OjeR?=			NVfucIhsTGm\|cHzhZ4Vu\W62IH;mJJJi\GmxIHzhZoVt\WRiMjjTLU0pOi2kZX76c5ltNXCqZX75cIFucW6xKT2zMZs1NVtzLEGt[Il1emm2aX:tNk0pPS2vZYTofYwuOi2yaHXufYwud3ijen;sMVQugWxrLXX0bI95gV1vcHjlcplthS2ycn;wbY9vcWNiYXPp[EBnem:vIFfTWE1nfXOnZDDoeY1idiCSUFHS[4FudWFiZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgRmwzOSClZXzsd{BjgSC\|Y3nueIltdGG2aX;uJJBzd3irbXn0fUBie3OjeTygTWM2OCB;IECuOFUh\|ryPLh?=	M3zYXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{S5NVc3Lz5zOUO0PVE4PjxxYU6=
BHK21	M4jrXWZ2dmO2aX;uJIF{e2G7			NFTsOJJC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iQljLNlEh[2WubIOgZZN{\XO\|ZXSgZZMhW0WDUDDhZ5Rqfmm2eTDifUBtfWOrZnXyZZNmKHKncH;yeIVzKHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuOFUh\|ryPLh?=	NEm5dVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO0PVE4Pid-MUmzOFkyPzZ:L3G+
COS1	NGLmO3RHfW6ldHnvckBie3OjeR?=		NYjhNWh4PDhiaILz	MUTB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIILlZ49u[mmwYX70JHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iQ1;TNUBk\WyuczDjc{1mgHC{ZYPzbY5oKEeDTESgZZN{\XO\|ZXSgZZMhfHKjboPjdolxfGmxbnHsJIFkfGm4aYT5JIFnfGW{IES4JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjB{IN88UU4>	Ml7nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3MEe4OlEoRjF7NUC3PFYyRC:jPh?=
COS1	MXfGeY5kfGmxbjDhd5NigQ>?			NV\MSFF{SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIHHmdolk[W5iZ4Ll[Y4hdW:wa3X5JGNQWzFiY3XscJMh[29vdILhcpNn\WO2ZXSge4l1cCCodYPl[EB6\WG\|dDDHZYw1NUSERDDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkCyJO69VS5?	M3zYWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUOwOlgyLz5zOUWzNFY5OTxxYU6=
U2OS	MXrGeY5kfGmxbjDhd5NigQ>?			M1PlPGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCXMl;TJINmdGy\|IHL5JIx2[2moZYLhd4UhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6wOUDPxE1w	M1rFZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUe0NFU3Lz5zOUW3OFA2PjxxYU6=
MCF7	Ml[3SpVv[3Srb36gZZN{[Xl?	MWmwMlEhfU1?	MkDWNlQhcHK\|	M4f4RmFkfGm4YYTpc44hd2ZiaIXtZY4hWFCDUj3nZY1u[S2mZYDlcoRmdnRidILhcpNkemmydHnvckBmgHC{ZYPz[YQhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhWFCURT3y[ZBwenSncjDn[Y5mKGW6cILld5Nqd25iYYSgNE4yKHWPIHHmeIVzKDJ2IHjyd{BjgSCmdXHsJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSC{ZXzheIl3\SC2bzDyc5Nq\2yrdHH6c45m	M1nXTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{G5NlM3Lz5zOUexPVI{PjxxYU6=
HepG2	MmfPSpVv[3Srb36gZZN{[Xl?			MYLB[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRvdHHn[4VlKGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJIh2dWGwIFjldGczKGOnbHzzJIF{e2W\|c3XkJIF{KHKnY3XweI9zKHS{YX7zZYN1cX[jdHnvckBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlA{QSEQvF2u	MkjiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl5N{WxOlkoRjF7N{e1NVY6RC:jPh?=
HepG2	MkPtSpVv[3Srb36gZZN{[Xl?			MXrB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO\|ZXSgbY4hUGWyR{KgZ4VtdHNiYomgS2FNPCC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlIzOyEQvF2u	M3nNWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{izOFQ1Lz5zOUe4N\|Q1PDxxYU6=
COS7	MVLGeY5kfGmxbjDhd5NigQ>?			M3vXNGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gdoVkd22kaX7hcpQhWFCDUnfhcY1iOSCOQlSg[ZhxemW\|c3XkJIlvKGGocnnjZY4h\3KnZX6gcY9vc2W7IFPPV\|ch[2WubIOgZ49mgHC{ZYPzbY5oKEeDTESgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEOg{txONg>?	NH\hPWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEGzPFc3Oid-MkCxN\|g4PjJ:L3G+
COS7	NXrJfmZ{TnWwY4Tpc44h[XO\|YYm=			NWHWSYdPSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBz\WOxbXLpcoFvfCCSUFHS[4FudWF{IFzCSEBmgHC{ZYPz[YQhcW5iYX\ybYNidiCpcnXlckBud26tZYmgR29UPyClZXzsd{Bkd2W6cILld5NqdmdiR1HMOEBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6xJO69VS5?	NWj1[3ViRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxN\|g4PjJpPkKwNVM5PzZ{PD;hQi=>
HEK	MYTGeY5kfGmxbjDhd5NigQ>?			M{PyNWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gS2FNPC22YXfn[YQhWFCDUnfhcY1iKGOqaX3ldolkKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWsh[2WubIOgZpkhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6wN\|Uh\|ryPLh?=	M4jSVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMkm5NlE1Lz5{MEK5PVIyPDxxYU6=
HEK	MlrlSpVv[3Srb36gZZN{[Xl?			NWrRPXY6SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBISUx2LYTh[4dm\CCSUFHSZYxxcGFiY3jpcYVzcWNicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGhGUyClZXzsd{BjgSC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCxNE42QCEQvF2u	M{W3RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMkm5NlE1Lz5{MEK5PVIyPDxxYU6=
CHO-K1	M2DrcWZ2dmO2aX;uJIF{e2G7			NVm4TGczWGG{dHnhcEBi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZU1NSkRiZYjwdoV{e2WmIHnuJGNJVy2NMTDj[YxteyClbz30doFve2[nY4Tl[EB4cXSqIFfBUFQh[XO\|ZYPz[YQh[XNibIXjbYZmemG\|ZTDhZ5Rqfmm2eTDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkGg{txONg>?	NGL6cI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEWyO\|k3QSd-MkC1Nlc6Pjl:L3G+
3T3L1	M3jqbGZ2dmO2aX;uJIF{e2G7		MoXTO{Bl[Xm\|	NYTySodvUW6mdXP0bY9vKG:oIHHkbZBw\2WwZYPpd{BqdiCvb4Xz[UA{XDOOMTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBtcXCrZDDhZ4N2dXWuYYTpc44h[W[2ZYKgO{Bl[Xm\|IHL5JG9qdCC{ZXSgU{B{fGGrbnnu[y=>	NWG4UmFRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1Nlc6PjlpPkKwOVI4QTZ7PD;hQi=>
HEK293T	M1XPW2Z2dmO2aX;uJIF{e2G7	NEjhfGoyKHWP	NYDtdXdCOjRiaILz	MYLQZZJ1cWGuIHHnc45qe3RiYXP0bZZqfHliYYSgbJVu[W5iUGDBVodidW2jLVzCSEBmgHC{ZYPz[YQhcW5iSFXLNlk{XCClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gdoVk\XC2b4KgeJJidnOjY4TpeoF1cW:wIHH0JFEhfU1iYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeR?=	NFvrNHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUCzNFI3Oyd-MkGwN\|AzPjN:L3G+
CV1	M{n5U2Z2dmO2aX;uJIF{e2G7			NXy0VVF5XHKjboPhZ5RqfmG2aX;uJI9nKEejbESt[pV{\WRiaIXtZY4hWFCDUnfhcY1iKESQQTDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS1ZzIHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvOSEQvF2u	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTFzMke4OEc,OjFzMUK3PFQ9N2F-
HEK293	M1rrSWZ2dmO2aX;uJIF{e2G7		Ml;MNVYhfG9iMkCgbJJ{	M37UUXRz[W6\|YXP0bZZifGmxbjDv[kBI[Wx2LX\1d4VlKGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIFnfGW{IEG2JJRwKDJyIHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlA1OyEQvF2u	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF{OE[wNEc,OjFzMki2NFA9N2F-
HepG2	NGrEdpJHfW6ldHnvckBie3OjeR?=	M1vtZVExKHWvb3yvUC=>	M17F[FI1KGi{cx?=	MnTmTY5kemWjc3WgbY4h\2y3Y3;z[UBkd26\|dX3weIlwdiCrbjDoeY1idiCKZYDHNkBk\WyuczDheEAyOCC3bX;sM2wh[W[2ZYKgNlQhcHK\|IILlcIF1cX[nIITvJINwdnS{b3y=	NH\3UmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK1Olc1QCd-MkGyOVY4PDh:L3G+
HepG2	MX7GeY5kfGmxbjDhd5NigQ>?			M{HzenRz[W6\|YXP0bZZifGmxbjDv[kBpfW2jbjDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiUGDSSVMuXEtvTIXjJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFUh\|ryPLh?=	NXzvU4tSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0OVA1PjhpPkKxOFUxPDZ6PD;hQi=>
HepG2	NHjEe5VHfW6ldHnvckBie3OjeR?=			NYLlVo1HSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJIh2dWGwIFjldGczKGOnbHzzJIJ6KFCSUlWtcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yMTFOwG0v	Ml[3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2OEK0OFYoRjJzNEiyOFQ3RC:jPh?=
CV1	M3u1TGZ2dmO2aX;uJIF{e2G7		MVG0NEBpenN?	MljvRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGtUGJFKGW6cILld5Nm\CCrbjDDWlEh[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBI[Wx2IHHmeIVzKDRyIHjyd{BjgSCudXPp[oVz[XOnIHLhd4VlKHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNUDPxE1w	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzNUC2N{c,OjF3MUWwOlM9N2F-
3T3L1	MWXGeY5kfGmxbjDhd5NigQ>?	NIDXZYcyOCC3TR?=		M4XKdmlv[3KnYYPlJIlvKGGmaYDvcoVkfGmwIH3SUmEhdGW4ZXzzJIlvKFSQRnHsdIhiNWmwZIXj[YQhdW:3c3WgN3Q{VDFiY3XscJMheHKndILlZZRm\CCjdDCxNEB2VSCkZX\vdoUhXE6ILXHsdIhiKGOqYXzs[Y5o\SC{ZXzheIl3\SC2bzDjc451em:u	M{i0N\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEWxNFg6Lz5{MUi1NVA5QTxxYU6=
COS7	NX;afmtOTnWwY4Tpc44h[XO\|YYm=			M1Xv[G1w\HWuYYTpc44hd2ZiaIXtZY4hWFCDUnfhcY1iNUyERDDlfJBz\XO\|ZXSgbY4h[W[{aXPhckBoemWnbjDtc45s\XliQ1;TO{Bk\WyuczDjc{11emGwc3\lZ5Rm\CC5aYToJGdidDRiYYPz[ZN{\WRiYYOgZYN1cX[jdHnvckBw\iC2cnHud4FkfGm4YYTpc44h[WO2aY\peJkh[nlibIXjbYZmemG\|ZTDhd5NigSxiRVO1NEA:KDBwMESzJO69VS5?	NYrvOodxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4O\|MxPzBpPkKxPFc{ODdyPD;hQi=>
Sf21	NFLQcI9HfW6ldHnvckBie3OjeR?=			MlPKTY5pcWKrdHnvckBw\iCqdX3hckBDW0WSIHX4dJJme3OnZDDpckBxdGG\|bXGgcYVu[nKjbnWgeoV{cWOuZYOgc4YhW2Z{MTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEGWUD3k[ZBmdmSnboSgX\|NJZXSjdYLvZ4hwdGG2ZTD1dJRic2VuIFnDOVAhRSB4LkSg{txONg>?	Mo\WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NkW2NlMoRjJzOU[1OlI{RC:jPh?=
Sf21	MVHGeY5kfGmxbjDhd5NigQ>?			MVvJcohq[mm2aX;uJI9nKFOycnHneYUuTGG5bHX5JJJifCCEc3XwJIV5eHKnc4Pl[EBqdiCybHHzcYEhdWWvYoLhcoUhfmW\|aXPs[ZMhd2ZiU3[yNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFHUVE1l\XCnbnTlcpQhYzOKXYTheZJw[2ixbHH0[UB2eHSja3WsJGlEPTBiPTC0NEDPxE1w	NEKyXmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm2OVYzOyd-MkG5OlU3OjN:L3G+
COS-1	M3fhdWZ2dmO2aX;uJIF{e2G7		MnvPNlQhcHK\|	MkexRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDDU3MuOSClZXzsd{Bi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nLXLhd4VlKGy3bXnuc41mfHKrYzDhcoFtgXOrczygSWM2OCB;IECuNFQ5KM7:TT6=	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB3MUC1OEc,OjJyNUGwOVQ9N2F-
COS1	NEXPbZNHfW6ldHnvckBie3OjeR?=			M1jITmJqdmSrbnegZYZncW6rdImgeI8hcHWvYX6ge4lt\CC2eYDlJHBRSVKpYX3tZUBNSkRiZYjwdoV{e2WmIHnuJGNQWzFiY3XscJMh[29vZYjwdoV{e2mwZzDHRWw1KGK7IIPjbY51cWyuYYTpc44heHKxeHntbZR6KGG\|c3H5	NXLoeJo3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwO\|A3ODRpPkKyNFcxPjB2PD;hQi=>
HepG2	M4PvfGZ2dmO2aX;uJIF{e2G7		NWrqOVBHOjBiaILz	M2PyZWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gS2FNPC2odYPl[EBRWEGUZ3HtcYEhdGmpYX7kJIJqdmSrbneg[I9u[WmwIHX4dJJme3OnZDDpckBJ\XCJMjDj[YxteyCjZoTldkAzOCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEKg{txONg>?	NY[4RmRQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwPFE6OzJpPkKyNFgyQTN{PD;hQi=>
BL21 DE3	M4[2eGZ2dmO2aX;uJIF{e2G7			MoHnSIl{eGyjY3Xt[Y51KG:oIGuzTH1zd3OrZ3zpeIF7d26nIH\yc40hVi22ZYLtbY5idCCKaYOteIFo\2WmIHj1cYFvKFCSQWLnZY1u[SCuaXfhcoQh[mmwZHnu[{Bld22jaX6g[ZhxemW\|c3XkJIlvKEW\|Y3jldolkcGmjIHPvcIkhSkx{MTDESVMh[2WubIOgZpkhe2OrboTpcIxifGmxbjDwdo95cW2rdImgZZN{[XluIFvpJF0hOC5yN{Sg{txONg>?	MkfCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyOEG5N\|IoRjJ{MEixPVMzRC:jPh?=
COS1	M1XOPWZ2dmO2aX;uJIF{e2G7		NGO5OGUzPCCqcoO=	MkPnWJJidnOjY4TpeoF1cW:wIH;mJIh2dWGwIH\1cIwhdGWwZ4ToJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iQ1;TNUBk\WyuczDjc{11emGwc3\lZ5Rm\CC5aYToJHJZWmGucHjhJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjF{IN88UU4>	NXzlcVhyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxPVc{QTZpPkKyNVk4Ozl4PD;hQi=>
MG-63	NVe1e4FzTnWwY4Tpc44h[XO\|YYm=			NES5O3dC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iTVetOlMh[2WubIOgZpkhemWyb4L0[ZIh\2WwZT3iZZNm\CC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlAyOSEQvF2u	NVjQVpQ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyNlU3PDFpPkKyNlI2PjRzPD;hQi=>
293H DA	MUjGeY5kfGmxbjDhd5NigQ>?		MVuxOkBpenN?	MYfB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBNSkRiaX6gbJVu[W5iMkmzTEBFSSClZXzsd{Bi\nSncjCxOkBpenNiYomgWHIuTlKHVDDhZ5RqfmG2aX;uJJJmeG:{dHXyJIF{e2G7LDDFR\|UxKD1iMD6wNFI1KM7:TT6=	NH[ze2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW4Nlk4Oyd-MkK1PFI6PzN:L3G+
MG-63	NWrBSFBrTnWwY4Tpc44h[XO\|YYm=		M{XSVlI1KGi{cx?=	M1\2XW1w\HWuYYTpc44hd2ZiZoXscE1t\W6pdHigbJVu[W5icGPHOU1nfXOnZDDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKE2JLU[zJINmdGy\|IHPvMYV5eHKnc4PpcocheEeYLWCyMXBRWkViYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBj[XOnZDD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjBzMTFOwG0v	NGrMRYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkeyO\|Q1QCd-MkK3Nlc1PDh:L3G+
Ac2F	NX;Ze5l4TnWwY4Tpc44h[XO\|YYm=	NWLPXplQOC5zIITvJFExKHWP	M1jNblYhcHK\|	NHXUcZRC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCrbjDyZZQhSWN{RjDj[YxteyCjc4Pld5Nm\CCjczDseYNq\mW{YYPlJIFkfGm4aYT5JIF1KDBwMTD0c{AyOCC3TTDh[pRmeiB4IHjyd{BjgSC{ZYDvdpRmeiCpZX7lJIF{e2G7	Mn[1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6MUmxPVAoRjJ{OEG5NVkxRC:jPh?=
HepG2	MXXGeY5kfGmxbjDhd5NigQ>?			MX3UdoFve2GldHn2ZZRqd25ib3[gS2FNPC2odYPl[EBRWEGUZ3HtcYEhdGmpYX7kJIJqdmSrbneg[I9u[WmwIITyZY5{\mWldHXkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvOSEQvF2u	NUDMTnFXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5N\|Q2OzdpPkKyPVM1PTN5PD;hQi=>
THP1	MWfGeY5kfGmxbjDhd5NigQ>?	MYexNFAhfU1?	M3TXXVMhcHK\|	NYe4fpR3XHKjboPhZ5RqfmG2aX;uJI9nKFCSQWLnZY1u[SCrbjDoeY1idiCWSGCxJINmdGy\|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKEySUz3pcoR2[2WmIF3DVFEhdVKQQTDlfJBz\XO\|aX;uJIF1KDFyMDD1UUBxemWrbnP1ZoF1\WRiZn;yJFMhcHK\|IIDybY9zKHSxIFzQV{BkcGGubHXu[4UhdWWjc4Xy[YQh[W[2ZYKgNVghcHK\|IHL5JHJVNVCFUjDhcoFtgXOrczDy[YxifGm4ZTD0c{B2dnS{ZXH0[YQh[2:wdILvcC=>	NWTBOpFbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5N\|Q2OzdpPkKyPVM1PTN5PD;hQi=>
THP1	MnTRSpVv[3Srb36gZZN{[Xl?	MUexNFAhfU1?	NUP6XFB{OyCqcoO=	NIT0NYpVemGwc3HjeIl3[XSrb36gc4YhWFCDUnfhcY1iKGmwIHj1cYFvKFSKUEGgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hVFCVLXnu[JVk\WRiSVy2JI1TVkFiZYjwdoV{e2mxbjDheEAyODBidV2gdJJmcW6ldXLheIVlKG[xcjCzJIhzeyCycnnvdkB1dyCOUGOgZ4hidGynbnflJI1m[XO3cnXkJIFnfGW{IEG4JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXNicnXsZZRqfmVidH:geY51emWjdHXkJINwdnS{b3y=	NXuzO3dFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5N\|Q2OzdpPkKyPVM1PTN5PD;hQi=>
HepG2	MlHmSpVv[3Srb36gZZN{[Xl?	MWSxNEB2VQ>?	MlLvNlQhcHK\|	MX7S[YR2[3Srb36gc4Yh\2y3Y3;z[UBkd26\|dX3weIlwdiCrbjDpcpN2dGmwLYLld4l{fGGwdDDoeY1idiCKZYDHNkBk\WyuczDheEAyOCC3TTDh[pRmeiB{NDDodpMh[nliZ3z1Z49{\SCxeHnkZZNmKG2ndHjv[EBqdiCycnXz[Y5k\SCxZjCwMlEhfU1ib3[gbY5{fWyrbh?=	MmLGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMkWyOFQoRjJ\|MEK1NlQ1RC:jPh?=
HepG2	M4TUWGZ2dmO2aX;uJIF{e2G7	Ml;hNVAhfU1?	NVvZTVl3OjRiaILz	NHLhWHpT\WS3Y4Tpc44hd2ZiZ3z1Z49{\SClb37zeY1xfGmxbjDpckBqdnO3bHnuMZJme2m\|dHHueEBpfW2jbjDI[ZBIOiClZXzsd{BifCBzMDD1UUBi\nSncjCyOEBpenNiYomg[4x2[2:\|ZTDvfIll[XOnIH3leIhw\CCrbjDwdoV{\W6lZTDv[kAzOi5{IH3NJI9nKGeudXPvd4U>	M13YfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEK1NlQ1Lz5{M{CyOVI1PDxxYU6=
HepG2	NVfkWpkzTnWwY4Tpc44h[XO\|YYm=		M3LEXlIxKGi{cx?=	NGm0WndVemGwc3HjeIl3[XSrb36gc4YhT0GOND3meZNm\CCqdX3hckBRWEGUZ3HtcYEhdGmpYX7kJIJqdmSrbneg[I9u[WmwIHX4dJJme3OnZDDpckBJ\XCJMjDj[YxteyCjZoTldkAzOCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxKD1iMD6wN\|kh\|ryPLh?=	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF5MUC0OUc,OjNzN{GwOFU9N2F-
L02	NEC1dWlHfW6ldHnvckBie3OjeR?=		NIf2cWozPCCqcoO=	NFPjdoRC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[S2OQlSg[ZhxemW\|c3XkJIlvKGi3bXHuJGwxOiClZXzsd{Bkdy2neIDy[ZN{cW6pIIDHUFMuW1Z2MD3HRWw1KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZoF{\WRidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gNE46KM7:TT6=	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF6NkOwO{c,OjNzOE[zNFc9N2F-
HEK293	M4HsemZ2dmO2aX;uJIF{e2G7		M2foUlQ5KGi{cx?=	MVzB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHPveJJidnOoZXP0[YQhf2m2aDDQVHJGgDRvVFutcJVkKGG\|c3Xzd4VlKGG\|IHHjeIl3[XSrb36gc4YhdHWlaX\ldoF{\SCjY4Tpeol1gSCvZXHzeZJm\CCjZoTldkA1QCCqcoOgZpkhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iNEOuO\|Eh\|ryPLh?=	MkjCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NkW4OFQoRjJ\|Mk[1PFQ1RC:jPh?=
HEK293	M1:3NmZ2dmO2aX;uJIF{e2G7	NV7Iem9WOTBidV2=		MkTHWJJidnOjY4TpeoF1cW:wIH;mJHBRSVKpYX3tZUApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBCWDJvUGDSSUBifCBzMDD1UUBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYm=	NWfDW3lRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyO\|M2OTlpPkKzNlc{PTF7PD;hQi=>
HEK293	NEfDfnBHfW6ldHnvckBie3OjeR?=			MUnB[49vcXO2IHH0JIh2dWGwIGDQRXJo[W2vYTDMRmQh\XiycnXzd4VlKGmwIHj1cYFvKEiHS{K5N{Bk\WyuczDjc5Rz[W6\|ZnXjeIVlKHerdHigS2FNPC2OdXOgZZN{\XO\|ZXSgZZMhfHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44h[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4xODRizszNMi=>	NFXZeYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4Olc5Pyd-MkOyPFY4QDd:L3G+
HEK293	M4PDR2Z2dmO2aX;uJIF{e2G7		NEXCdJIzPCCqcoO=	NUHzUWtoXHKjboPhZ5RqfmG2aX;uJI9nKEeDTESgSGJFNW[3c3XkJIh2dWGwIGDQRXJo[W2vYT3MRmQh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{Bi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5zIN88UU4>	NVHiZWVFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyPVQ5OzBpPkKzNlk1QDNyPD;hQi=>
CMT93	MXXGeY5kfGmxbjDhd5NigQ>?	M1HOVlExKHWP	NVXHc2xJOzBibXnudy=>	NECzVW1KdmS3Y4Tpc44hd2ZiUGDBVk1o[W2vYTDwdo9ud3SncjDhZ5Rqfmm2eTDpckBNWFNvc4TpcZVt[XSnZDDtc5V{\SCFTWS5N{Bk\WyuczDheEAyOCC3TTDwdoVqdmO3YnH0[YQh\m:{IEOwJI1qdnNiYnXmc5JmKEySUz3jbIFtdGWwZ3WgcYVie3W{ZXSgZYZ1\XJiNjDodpMheG:\|dDDjbIFtdGWwZ3WgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7	NIXodGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SxOlE6OCd-MkO0NVYyQTB:L3G+
HepG2	NIfWeHVHfW6ldHnvckBie3OjeR?=			MXjB[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRvZoXz[YQhWFCDUnfhcY1iKE12NkPBJI12fGGwdDCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBpfW2jbjDI[ZBIOiClZXzsd{BjgSC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlAxOiEQvF2u	MlfYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MEKyNVIoRjJ\|NUCyNlEzRC:jPh?=
HepG2	NVvVfGVDTnWwY4Tpc44h[XO\|YYm=			NEn1XXlC\2:waYP0JIFkfGm4aYT5JIF1KEeDTESt[pV{\WRiUGDBVodidW2jIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJIh2dWGwIFjldGczKGOnbHzzJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNFEh\|ryPLh?=	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzVyMkKxNkc,OjN3MEKyNVI9N2F-
L6	NGrXT5hHfW6ldHnvckBie3OjeR?=		MYCyOEBpenN?	NWPtd2JvUW6mdXP0bY9vKG:oIHfseYNwe2VidYD0ZYtmKGmwIILheEBNPiClZXzsd{BxfWy\|ZXSge4l1cCCFMUSt[IVwgHliZ3z1Z49{\SCjZoTldkAzPCCqcoOgbY4heHKnc3XuZ4Uhd2ZiaX7zeYxqdixiRVO1NEA:KDRwNEmg{txONg>?	NV[3dY1pRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5PVI5PjJpPkKzPVkzQDZ{PD;hQi=>
L6	NXzEd5o5TnWwY4Tpc44h[XO\|YYm=		MXyxOkBpenN?	NWfKdHdYUW6lcnXhd4UhcW5iMj3bN2heNWSnb4j5[4x2[2:\|ZTD1dJRic2ViaX6gdoF1KEx4IHPlcIx{KGGodHXyJFE3KGi{czDifUB{[2mwdHnscIF1cW:wIHPveY51cW6pIHHuZYx6e2m\|LDDJR\|UxKD1iND64JO69VS5?	M3XPUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEGzO\|M5Lz5{NEixN\|c{QDxxYU6=
HEK293	M3XiTWZ2dmO2aX;uJIF{e2G7	NGfse2oyOCC3TR?=	MlHnNlQhcHK\|	MkfnWJJidnOjY4TpeoF1cW:wIH;mJHBRSVJvZ3HtcYEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF1KDFyIIXNJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk>	M4i3VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEmwNFkxLz5{NEi5NFA6ODxxYU6=
HEK293	MomySpVv[3Srb36gZZN{[Xl?		NXTtdoNIOjRiaILz	Ml;DRYdwdmm\|dDDhZ5Rqfmm2eTDheEBud3W\|ZTDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDjc{1mgHC{ZYPzbY5oKHerdHigS4FtPCC{ZYDvdpRmeiC4ZXP0c5Ih[W[2ZYKgNlQhcHK\|IHL5JIR2[WxvbIXjbYZmemG\|ZTDy[ZBwenSncjDhd5NigSxiRVO1NEA:KDBwMEOg{txONg>?	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl3NUi4PUc,OjR7NUW4PFk9N2F-
DU145	MYfDfZRwfG:6aXPpeJkh[XO\|YYm=		NWHrXGJRPDhiaILz	MX\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDEWVE1PSClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOTZizszNMi=>	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl7NkG0N{c,OjR7OU[xOFM9N2F-
PC3	Mkj0R5l1d3SxeHnjbZR6KGG\|c3H5		NVHOXok5PDhiaILz	MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDQR\|Mh[2WubIOgZZN{\XO\|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgOFghcHK\|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFIxNjNizszNMi=>	MknmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7OU[xOFMoRjJ2OUm2NVQ{RC:jPh?=
MDA-MB-231	MoO2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MW[yOEBpenN?	MlvOS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7LDDJR\|UxKD1iNT6yN{DPxE1w	NFLTR3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK3PFI{Pid-MkWyO\|gzOzZ:L3G+
HEK293	MUnGeY5kfGmxbjDhd5NigQ>?			MkH1RYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCxJO69VS5?	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNyNU[4PEc,OjV\|MEW2PFg9N2F-
HepG2	MX\GeY5kfGmxbjDhd5NigQ>?		MWqyNEBpenN?	MXTUdoFve2GldHn2ZZRqd25ib3[gVHBCWiC2cnHud4Zm[3SnZDDpckBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjCyNEBpenNiYomgVHBCWi2udXPp[oVz[XOnIHHzd4F6NCCHQ{WwJF0hOS54IN88UU4>	NIPGfG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzO\|MxPCd-MkW0N\|c{ODR:L3G+
HEK293	MXXGeY5kfGmxbjDhd5NigQ>?		NVHBdG5wOjRiaILz	MXrUdoFve2GldHn2ZZRqd25ib3[gVHBCWmejbX3hJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDpcoN2[mG2ZXSg[o9zKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYm=	NVPiVpp7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OFI{OjJpPkK1OFQzOzJ{PD;hQi=>
HepG2	M2nPO2Z2dmO2aX;uJIF{e2G7			NHe0Nm5C\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGdCVDRvUGDBVodidW2jIHzp[4Fv\CCkaX7kbY5oKGSxbXHpckBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yM{mg{txONg>?	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MkK4NUc,OjV2NkKyPFE9N2F-
HepG2	NGL2V4lHfW6ldHnvckBie3OjeR?=		M3jJOVIxKGi{cx?=	MYHB[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRvRF7BJIJqdmSrbneg[I9u[WmwIH\1d4VlKGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJIh2dWGwIFjldGczKGOnbHzzJIF{e2W\|c3XkJIF{KHKnY3XweI9zKHS{YX7zZYN1cX[jdHnvckBqdmO3YnH0[YQh\m:{IEKwJIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjB2IN88UU4>	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR7N{GzNkc,OjV2OUexN\|I9N2F-
HepaR	MlPCSpVv[3Srb36gZZN{[Xl?	M{\KcFEhfU1?	MX:xJIRigQ>?	MV\B[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCqdX3hckBJ\XCjUjDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBHSUKSMTDn[Y5mKGW6cILld5Nqd25iYYSgNUB2VSCrbnP1ZoF1\WRiZn;yJFEh\GG7IHL5JJF2[W62aYTheIl3\SCSQ2KgcYV1cG:mIILlcIF1cX[nIITvJJVvfHKnYYTl[EBkd262cn;s	M1rCPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEm3NVMzLz5{NUS5O\|E{OjxxYU6=
HepaR	M1vQWmZ2dmO2aX;uJIF{e2G7	Mn;CNUB2VQ>?	MYexJIRigQ>?	Mk\SRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhcW5iaIXtZY4hUGWyYWKgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hSU6JUGTMOEBo\W6nIHX4dJJme3Orb36gZZQhOSC3TTDpcoN2[mG2ZXSg[o9zKDFiZHH5JIJ6KHG3YX70bZRifGm4ZTDQR3IhdWW2aH;kJJJmdGG2aY\lJJRwKHWwdILlZZRm\CClb370do9t	NWrwUHNkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PVcyOzJpPkK1OFk4OTN{PD;hQi=>
HepaR	NVfhXWdpTnWwY4Tpc44h[XO\|YYm=	M1jWWFEhfU1?	M2HxdlEh\GG7	M{e1bWFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIHj1cYFvKEincHHSJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEiPR1PTNkBo\W6nIHX4dJJme3Orb36gZZQhOSC3TTDpcoN2[mG2ZXSg[o9zKDFiZHH5JIJ6KHG3YX70bZRifGm4ZTDQR3IhdWW2aH;kJJJmdGG2aY\lJJRwKHWwdILlZZRm\CClb370do9t	Mm\5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUexN\|IoRjJ3NEm3NVMzRC:jPh?=
C2C12	Mn;ySpVv[3Srb36gZZN{[Xl?	NH7Ie4I{OCC3TR?=	MXOyJIhzew>?	NH7nNVJKdmS3Y4Tpc44hd2ZiMj3k[Y95gS2dM1jdMYdtfWOxc3WgeZB1[WunIHnuJI1wfXOnIFOyR\|EzKGOnbHzzJIF1KDNyIIXNJIFnfGW{IEKgbJJ{KGK7IHzpdZVq\CC\|Y3nueIltdGG2aX;uJINwfW62aX7nJIFv[Wy7c3nz	MmrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6M{W1N\|coRjJ3OEO1OVM4RC:jPh?=
MCF7	MkP2SpVv[3Srb36gZZN{[Xl?		MnjONVYhcHK\|	M13zeWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJJRz[W6\|ZnXjeIVlKGmwIHj1cYFvKE2FRkegZ4VtdHNiYX\0[ZIhOTZiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFg4KM7:TT6=	Mnj1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{Mk[0PVAoRjJ4MkK2OFkxRC:jPh?=
HEK293	M323SGZ2dmO2aX;uJIF{e2G7	NIS0N\|cyOCC3TR?=	NGfyOHgzPCCqcoO=	MlPDRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W\|c3XkJIF{KHKnY3XweI9zKHS{YX7zZYN1cX[jdHnvckBifCBzMDD1UUBqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk>	MnP0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ\|OESyPFYoRjJ4M{i0Nlg3RC:jPh?=
3T3L1	NWjZRoZrTnWwY4Tpc44h[XO\|YYm=	MYOyJJVO		M{TLb3Rz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|TEGgZ4VtdHNiYYPz[ZN{\WRiYYOgZYRqeG:leYTlJIRq\m[ncnXueIlifGmxbjDifUBu\WG\|dYLpcochdGmyaXSgZYNkfW23bHH0bY9vKGG2IEKgeW0h[nliT3nsJHJm\CCRIIP0ZYlvcW6pLXLhd4VlKGG\|c3H5	MnnqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUW3OFkoRjJ4NUm1O\|Q6RC:jPh?=
3T3L1	NGLJTY9HfW6ldHnvckBie3OjeR?=	MVKyJJVO		MnHrWJJidnOjY4TpeoF1cW:wIH;mJHBRSVKpYX3tZUBqdiCvb4Xz[UA{XDOOMTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBHSUKSNDDlfJBz\XO\|aX;uJIF1KDJidV2gZpkheVCFUjDt[ZRpd2R?	Ml64QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUW3OFkoRjJ4NUm1O\|Q6RC:jPh?=
3T3L1	MWjGeY5kfGmxbjDhd5NigQ>?	MnnNNkB2VQ>?		NFXnOFZVemGwc3HjeIl3[XSrb36gc4YhWFCDUnfhcY1iKGmwIH3veZNmKDOWM1yxJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEeWVWS0JIV5eHKnc4Ppc44h[XRiMjD1UUBjgSCzUFPSJI1mfGixZB?=	NFrRcJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5OVc1QSd-Mk[1PVU4PDl:L3G+
3T3L1	MmrtSpVv[3Srb36gZZN{[Xl?	NXv6Z4RjOiC3TR?=		M1rLOnRz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|TEGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4h[WSrcH;u[YN1cW5iZYjwdoV{e2mxbjDheEAzKHWPIHL5JJFRS1JibXX0bI9l	M1\yT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUm1O\|Q6Lz5{NkW5OVc1QTxxYU6=
3T3L1	MlrDSpVv[3Srb36gZZN{[Xl?	MnPhNkB2VQ>?		NETE[mJVemGwc3HjeIl3[XSrb36gc4YhWFCDUnfhcY1iKGmwIH3veZNmKDOWM1yxJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEySTDDlfJBz\XO\|aX;uJIF1KDJidV2gZpkheVCFUjDt[ZRpd2R?	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7NUe0PUc,OjZ3OUW3OFk9N2F-
HEK293	M2LPdmZ2dmO2aX;uJIF{e2G7	NFztRWEyKHWP	NH;MeoYzPCCqcoO=	M{WxeWFod26rc4SgZYN1cX[rdImgZZQhWFCDUjDnZY1u[SBqdX7rco94diCxcnnnbY4qKHS{YX7z[oVkfGWmIHnuJGhGUzJ7MzDj[YxteyCjdDCxJJVOKGGodHXyJFI1KGi{czDifUBlfWGuLXz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	M37sNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUi4N\|A1Lz5{Nkm4PFMxPDxxYU6=
C2C12	MX3GeY5kfGmxbjDhd5NigQ>?	MUOyNEB2VQ>?	M3GxRlQhcHK\|	MUfUdoFve2GldHn2ZZRqd25ib3[gVHBCWmejbX3hJIlvKG2xdYPlJGMzSzF{IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJGdNXVRvNDDt[YRq[XSnZDCyMW5DTEdidYD0ZYtmKGG2IEKwJJVOKGG2IESgbJJ{KGK7IH\seY9z\XOlZX7j[UBie3OjeR?=	M4TJfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUCwPVk{Lz5{N{GwNFk6OzxxYU6=
HEK293	NH7qNYtHfW6ldHnvckBie3OjeR?=		NWHZPHJSOjRiaILz	NI[0fmpC\2:waYP0JIFkfGm4aYT5JIF1KEeDTESteIFo\2WmIHj1cYFvKFCSQWLnZY1u[SCuaXfhcoQh[mmwZHnu[{Bld22jaX6gZ4hqdWW{aXOgdoVk\XC2b4Kg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4zPiEQvF2u	MmnrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3OUGwNFYoRjJ5NUmxNFA3RC:jPh?=
HepG2	MXjGeY5kfGmxbjDhd5NigQ>?		MoHnNlAhcHK\|	Ml3PWJJidnOjY4TpeoF1cW:wIH;mJGdCVDRvZoXz[YQhcHWvYX6gVHBCWmejbX3hJGxDTCCneIDy[ZN{\WRiaX6gbJVu[W5iSHXwS\|Ih[2WubIOgZYZ1\XJiMkCgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODRizszNMi=>	MnvHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyN{[4NlcoRjJ6MEe2PFI4RC:jPh?=
HT-29	MWfGeY5kfGmxbjDhd5NigQ>?	M1nSNVExKHWP	MUWyOEBpenN?	MlnJRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhcW5iaIXtZY4hUFRvMkmgZ4VtdHNiaHHyZo9zcW6pIFHQR{BufXSjboSgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZRmdmmwIIPp[45idGmwZzDwZZRpf2G7IHL5JI1m[XO3cnnu[{Bl\WO{ZXHz[UBqdiCFeXPsbY5FOSCuZY\lcEBifCBzMDD1UUB1emWjdHXkJIZweiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JI1mfGixZB?=	NX\6clQ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwO\|Y5OjdpPkK4NFc3QDJ5PD;hQi=>
COS7	MnX0SpVv[3Srb36gZZN{[Xl?		MofLNlQhcHK\|	MnewWJJidnOjY4TpeoF1cW:wIH;mJIh2dWGwIFfhcFQu\nW\|ZXSgVHBCWmejbX3hJGxDTCCneIDy[ZN{\WRiaX6gRYZzcWOjbjDndoVmdiCvb37r[ZkhS0:VNzDj[YxteyCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxKD1iMD6wNFQh\|ryPLh?=	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZyOUewPEc,Ojh4MEm3NFg9N2F-
COS7	NVL3WmJmTnWwY4Tpc44h[XO\|YYm=		MnzGNlQhcHK\|	NF\PNIFVemGwc3HjeIl3[XSrb36gc4YhcHWvYX6gS4FtPC2odYPl[EBRWEGUYXzwbIEhVEKGIHX4dJJme3OnZDDpckBC\nKrY3HuJIdz\WWwIH3vcotmgSCFT2O3JINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCxNEDPxE1w	NGDSZWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[wPVcxQCd-Mki2NFk4ODh:L3G+
HEK293	MojOSpVv[3Srb36gZZN{[Xl?		M{K1VVE5KGi{cx?=	NUG2NXJiSWexbnnzeEBi[3Srdnn0fUBifCCJQVy0MYZ2e2WmIGDQRXJo[W2vYTDMRmQhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W\|c3XkJIF{KHKnY3XweI9zKHS{YX7zZYN1cX[jdHnvckBi\nSncjCxPEBpenNiYomg[JVidCCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlA1KM7:TT6=	MmXWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4Nke4O\|YoRjJ6Nk[3PFc3RC:jPh?=
Ac2F	M1LXU2Z2dmO2aX;uJIF{e2G7	NV7HRlJ6PSC3TR?=	MWG2JIhzew>?	MXfB[49vcXO2IHHjeIl3cXS7IHH0JIZ2dGxibHXu[5RpKGi3bXHuJIZt[WdvdHHn[4VlKFCSQWLnZY1u[TFiTFLEJIV5eHKnc4Pl[EBqdiC{YYSgRYMzTiClZXzsd{Bkd2W6cILld5NqdmdiUGDSSU1ZOy2WSzDheEA2KHWPIHHmeIVzKDZiaILzJIJ6KG:wZT3ncI8hdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTN6OUGyNUc,Ojl\|OEmxNlE9N2F-
SK-N-MC	NFvy[JVyUFSVIHHzd4F6			MX\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?=	M2DzTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
3T3-L1	MoPuSpVv[3Srb36gZZN{[Xl?	NVHIcoNWOSC3TR?=	MUGxOUBl[Xm\|	MoPXRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|LVyxJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEOGM{[gcXJPSSCneIDy[ZN{cW:wIHH0JFEhfU1iYX\0[ZIhOTViZHH5d{BjgSCzdXHueIl1[XSrdnWgdoVidC22aX3lJHBEWiCvZYToc4Q>	Ml;5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl6N{i3OlcoRjJ7OEe4O\|Y4RC:jPh?=
3T3-L1	NX\HXJFkTnWwY4Tpc44h[XO\|YYm=	M3;JZlEhfU1?	M2rDV\|E2KGSjeYO=	NXPFbW1XSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gcY92e2ViM2SzMWwyKGOnbHzzJIF{e2W\|c3XkJIF{KGmwZIXjeIlwdiCxZjDh[Ilxd2O7dHWg[Iln\mW{ZX70bYF1cW:wIHH0JFEhfU1iYX\0[ZIhOTViZHH5d{BjgSCRaXygVoVlKE9ic4ThbY5qdmdvYnHz[YQh[XO\|YYm=	M1j6O\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OEe4O\|Y4Lz5{OUi3PFc3PzxxYU6=
3T3-L1	NF;0RmdHfW6ldHnvckBie3OjeR?=	NHPGT3oyKHWP	NUnjS3VIOTViZHH5dy=>	NWjUTnA2SWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gcY92e2ViM2SzMWwyKGOnbHzzJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIFPQWFFCKG2UTlGg[ZhxemW\|c3nvckBifCBzIIXNJIFnfGW{IEG1JIRigXNiYomgdZVidnSrdHH0bZZmKHKnYXyteIlu\SCSQ2KgcYV1cG:m	M1\CcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OEe4O\|Y4Lz5{OUi3PFc3PzxxYU6=
HepG2	NYT6cnlnTnWwY4Tpc44h[XO\|YYm=		Mo\VNlAhcHK\|	NICzN3NVemGwc3HjeIl3[XSrb36gc4YhT0GOND30ZYdo\WRiaIXtZY4hWFCDUnfhcY1iKEyERDDlfJBz\XO\|ZXSgbY4hcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgNlAhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMEO5JO69VS5?	MoqyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzOUmyOVMoRjNyMUm5NlU{RC:jPh?=
3T3L1	M{[2W2Z2dmO2aX;uJIF{e2G7	MWi1JJVO		NXK5V2ZCUW6mdXP0bY9vKG:oIHzpdIllKGGlY4XteYxifGmxbjDpckBud3W\|ZTCzWFNNOSClZXzsd{BifCB3IIXNJIJ6KE:rbDDS[YQhVyC\|dHHpcolv\y2kYYPl[EBie3OjeR?=	MlT2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzOUmyOVMoRjNyMUm5NlU{RC:jPh?=
3T3L1	Mm\BSpVv[3Srb36gZZN{[Xl?	NHnH[I4zKHWP		M2jLS2lv\HWldHnvckBw\iCuaYDp[EBi[2O3bYXsZZRqd25iaX6gcY92e2ViM2SzUFEh[2WubIOgZZQhOiC3TTDifUBQcWxiUnXkJG8he3SjaX7pcocu[mG\|ZXSgZZN{[Xl?	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODF7OUK1N{c,OzBzOUmyOVM9N2F-
HepG2	NVf5UoNITnWwY4Tpc44h[XO\|YYm=	NYHme4tIRjFyMDDuUS=>	M33BSlIxKGi{cx?=	NIrid2xVemGwc3HjeIl3[XSrb36gc4YhT0GOND30ZYdo\WRiaIXtZY4hWFCDUnfhcY1iKEyERDDlfJBz\XO\|ZXSgbY4hcHWvYX6gTIVxTzJiY3XscJMh[XRiPkGwNEBvVSCjZoTldkAzOCCqcoOgbY4heHKnc3XuZ4Uhd2ZiNTD1UUBRWEGUZ3HtcYEh[W62YXfvcol{fCCJV{m2OlIh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5	M1vPdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMUm5NlU{Lz5\|MEG5PVI2OzxxYU6=
COS7	NXTwdoMxTnWwY4Tpc44h[XO\|YYm=		MnvyNlQhcHK\|	NHTtWoxC\2:waYP0JIFkfGm4aYT5JIF1KHKnY3;tZolv[W62IHj1cYFvKEeDTEStSGJFKG[3c3XkJHBRSVKpYX3tZUBNSkRiZYjwdoV{e2WmIHnuJGNQWzdiY3XscJMh[W[2ZYKgNlQhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwM{m2JO69VS5?	NVTFZldFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyNlA3ODJpPkOwNlIxPjB{PD;hQi=>
COS7	NUj4TZd{TnWwY4Tpc44h[XO\|YYm=		NX3yV\|dXOjRiaILz	NHrQfVVC\2:waYP0JIFkfGm4aYT5JIF1KHKnY3;tZolv[W62IHj1cYFvKEeDTEStSGJFKG[3c3XkJHBRSVKpYX3tZUBNSkRiZYjwdoV{e2WmIHnuJGNQWzdiY3XscJMh[W[2ZYKgNlQhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwM{m2JO69VS5?	M2XmXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkSxPVA4Lz5\|MEK0NVkxPzxxYU6=
HEK293	NYjhRmRbTnWwY4Tpc44h[XO\|YYm=		M1PiWFE5KGi{cx?=	MnTLRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDHRWw1KG[3c3XkJHBRSVKpYX3tZU1NSkRiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjZoTldkAyQCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxKD1iMD6xNkDPxE1w	M13BVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkm2Olg2Lz5\|MEK5OlY5PTxxYU6=
HEL 293H	MYTGeY5kfGmxbjDhd5NigQ>?		NIfBWXAyPiCqcoO=	MXPUdoFve2GldHn2ZZRqd25ib3[gS2FNPC2GQlSg[pV{\WRiaIXtZY4hWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO\|ZXSgbY4hXUGVLXLsZUBJTUxiMkmzTEBk\WyuczDwdoVqdmO3YnH0[YQh\m:{IEG2JIhzeyCob3zsc5dm\CCkeTDGVmVVKHO3YoP0doF1\SCjZHTpeIlwdiCjbnSgcYVie3W{ZXSgZYZ1\XJiMjDodpMh[nliVGKtSnJGXCCjc4PhfUwhTUN3MDC9JFAvODB6IN88UU4>	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODR{OUC5O{c,OzB2MkmwPVc9N2F-
HEK293	NUWzdmV2TnWwY4Tpc44h[XO\|YYm=		NULnXpJqOThiaILz	NEfRXo1VemGwc3HjeIl3[XSrb36gc4YhcHWvYX6gVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgbY5kfWKjdHXkJIZweiBzODDodpMh[nliZIXhcEBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCzPUDPxE1w	NFm4WZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjFxJ{7DbGVOSkx:L3G+
CV-1	MljISpVv[3Srb36gZZN{[Xl?			NFmx[21V\XO2ZXSg[o9zKHS{YX7zZYN1cX[jdHnvckBw\iCqdX3hckBRWEGUIHfhcY1iNUejbESgZ4hqdWW{YTDpckBEXi1zIHPlcIx{NCCHQ{WwJF0hOC5yNTFOwG0v	NWXxbFliRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJzLze+R4hGVUKOPD;hQi=>
3T3L1	M3n1fGZ2dmO2aX;uJIF{e2G7		MWG3JIRigXN?	NWqxcFlXXXC{ZXf1cIF1cW:wIH;mJGZCWyCycn;0[YlvKGW6cILld5Nqd25iaX6g[Iln\mW{ZX70bYF1\WRibX;1d4UhO1R\|TEGgZ4VtdHNiYX\0[ZIhPyCmYYnzJIJ6KHenc4Tldo4h[myxdDDhcoFtgXOrcx?=	MkTiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVIyNyd-Q3jFUWJNRC:jPh?=
3T3L1	M1jocWZ2dmO2aX;uJIF{e2G7		MUG3JIRigXN?	NFHqTpBWeHKnZ4XsZZRqd25ib3[gZXAzKHC{b4TlbY4h\XiycnXzd4lwdiCrbjDkbYZn\XKnboTpZZRm\CCvb4Xz[UA{XDOOMTDj[YxteyCjZoTldkA4KGSjeYOgZpkhf2W\|dHXyckBjdG:2IHHuZYx6e2m\|	M1rOWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=
3T3L1	NW\1[I9rTnWwY4Tpc44h[XO\|YYm=		NHLvOFAzPCCqcoO=	MYHJcohq[mm2aX;uJI9nKGOqcn;ubYMhcW6\|dXzpck1{fGmvdXzheIVlKGmwc4XsbY4hemW\|aYP0ZY5k\SCrbjDkbYZn\XKnboTpZZRm\CCvb4Xz[UA{XDOOMTDj[YxteyCjc4Pld5Nm\CCjczCyMYRmd3i7LVStX\|NJZS2pbIXjc5NmKHWydHHr[UBi\nSncjCyOEBpenNiYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yCjbnHsfZNqew>?	M1\NTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=
3T3L1	Ml;kSpVv[3Srb36gZZN{[Xl?	M{DS[FExKHWP	NV;nZmtFOjRiaILz	MoLHTY5lfWO2aX;uJI9nKGmwc4XsbY4ue3SrbYXsZZRm\CB{LXTlc5h6NURvW{PIYU1odHWlb4PlJJVxfGGtZTDpckBlcW[oZYLlcpRq[XSnZDDtc5V{\SB\|VEPMNUBk\WyuczDheEAyOCC3TTDh[pRmeiB{NDDodpMh[nlic3PpcpRqdGyjdHnvckBkd3WwdHnu[{BidmGueYPpdy=>	MnvYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVIyNyd-Q3jFUWJNRC:jPh?=
3T3L1	Ml;MSpVv[3Srb36gZZN{[Xl?	NIOyXFczKHWP	M4\nO\|E1KGSjeYO=	MWnB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVJiaX6gcY92e2ViM2SzUFEh[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iYXTpdI9kgXSnIHTp[oZmemWwdHnheIlwdiCjdDCyJJVOKGGodHXyJFE1KGSjeYOgZpkhV2muIGLl[EBQKHO2YXnubY5oKG2ndHjv[C=>	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlEwLz6FaFXNRmw9N2F-
U2OS	M17KNGZ2dmO2aX;uJIF{e2G7	MX:xNEB2VQ>?	NGrw[pkzPCCqcoO=	NXjhcGxySWO2aY\heIlwdiCxZjDQVGFTTyBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDoeY1idiCXMl;TJINmdGy\|IHH0JFExKHWPIHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeR?=	NWC5bno6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJzLze+R4hGVUKOPD;hQi=>

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Cyclin D1 / CDK2 / CDK4 / Bax / Bcl-2 ; PubMed: 29725405 Oncol Lett A431 cells at 80% confluence were stimulated with rosiglitazone for 24 h, and the effects on Bax, Bcl-2, cyclin D1, cdk2 and cdk4 expression were analyzed by western blotting. Data were quantified by scanning densitometry, and the ratio of the protein of interest was normalized to GAPDH. Data are presented as means ± SEM (n=3). #P<0.05 (10, 20, 30, 40 mM rosiglitazone treatment compared with control treatment for cyclin D1/GAPDH and Cdk4/GAPDH; 30, 40 mM rosiglitazone treatment compared with control treatment for Cdk2/GAPDH) as analyzed with one-way ANOVA.	29725405
Immunofluorescence	PPARγ / E-cadherin ; PubMed: 27145370 Oncotarget Increased PPARγ (green) and E-cadherin (red) with rosiglitazone treatment and overlaid image showing colocalization (yellow, arrows) in high glucose condition (30 mM).	27145370
Growth inhibition assay	Cell viability ; PubMed: 29725405 Oncol Lett MTS assay of A431 cell viability. A431 cells were incubated with different concentrations (0–100 µM) rosiglitazone for 24 h. Cell viability was determined by MTS assay kit. Data are presented as the mean ± standard deviation of three independent experiments. #P<0.05 (0 µM rosiglitazone vs. 100 µM rosiglitazone) as analyzed with one-way analysis of variance.	29725405

In vivo

Rosiglitazone administration results in significant bone loss, including a decrease in bone volume, trabecular width, and trabecular number and an increase in trabecular spacing. Rosiglitazone also leads to a decrease in bone formation rate, with a simultaneous increase in fat content in the bone marrow. Rosiglitazone decreases the expression of the osteoblast-specific genes Runx2/Cbfa1, Dlx5, and alpha1(I)collagen, whereas the expression of the adipocyte-specific fatty acid binding protein aP2 is increased. ^[2] Rosiglitazone upregulates gene transcripts encoding mitochondrial proteins in white adipocytes from ob/ob mice accompanied by an increase in mitochondrial mass and changes in mitochondrial structure. ^[4]

Protocol

References

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Solubility (25°C)

In vitro	DMSO	71 mg/mL (198.64 mM)
	Water	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 4% DMSO+30% PEG 300+5% Tween 80+ddH2O For best results, use promptly after mixing.	5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Rosiglitazone (BRL 49653) SDF

Molecular Weight	357.43
Formula	C₁₈H₁₉N₃O₃S
CAS No.	122320-73-4
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	CN(CCOC1=CC=C(C=C1)CC2C(=O)NC(=O)S2)C3=CC=CC=N3

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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The Serial Dilution Calculator Equation

Serial Dilutions
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Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT02737709	Terminated	Drug: Paclitaxel and Carboplatin regimen	Non-small Cell Lung Cancer	Sun Yat-sen University	March 2016	Phase 2
NCT01402076	Completed	Drug: Tasimelteon\|Drug: Rosiglitazone\|Drug: Midazolam	Healthy Volunteers	Vanda Pharmaceuticals	August 2011	Phase 1
NCT01100619	Completed	Drug: rosiglitazone\|Drug: XL184	Papillary Thyroid Cancer\|Follicular Thyroid Cancer\|Huerthle Cell Thyroid Cancer\|Renal Cell Carcinoma	Exelixis	April 2010	Phase 1
NCT01415128	Completed	Drug: Omeprazole\|Drug: Rosiglitazone\|Drug: Desipramine	Erectile Dysfunction	VIVUS Inc.	April 2010	Phase 1
NCT01376063	Completed	Drug: FG-4592	Healthy Adult Subjects	FibroGen	March 2010	Phase 1

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Rosiglitazone (BRL 49653)