Rosiglitazone (BRL 49653)

Catalog No.S2556

For research use only.

Rosiglitazone (BRL 49653) is a potent antihyperglycemic agent and a potent thiazolidinedione insulin sensitizer with IC50 of 12, 4 and 9 nM for rat, 3T3-L1 and human adipocytes, respectively. Rosiglitazone is a pure ligand of PPAR-gamma, and has no PPAR-alpha-binding action. Rosiglitazone modulates TRP channels and induces autophagy. Rosiglitazone prevents ferroptosis.

Rosiglitazone (BRL 49653) Chemical Structure

CAS No. 122320-73-4

Selleck's Rosiglitazone (BRL 49653) has been cited by 51 publications

Purity & Quality Control

Choose Selective PPAR Inhibitors

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Biological Activity

Description Rosiglitazone (BRL 49653) is a potent antihyperglycemic agent and a potent thiazolidinedione insulin sensitizer with IC50 of 12, 4 and 9 nM for rat, 3T3-L1 and human adipocytes, respectively. Rosiglitazone is a pure ligand of PPAR-gamma, and has no PPAR-alpha-binding action. Rosiglitazone modulates TRP channels and induces autophagy. Rosiglitazone prevents ferroptosis.
Targets
PPAR [1]
(Cell-free assay)
TRPC [5] Ferroptosis [6]
In vitro

Rosiglitazone markedly increase phosphorylation of threonine 172 within the α subunit of AMPK, with increased AMP:ATP ratio. Rosiglitazone has been reported to decrease cholesterol synthesis in a number of cell lines in a peroxisome proliferator-activated receptor γ-independent manner. Rosiglitazone activates both α1- and α2-containing AMPK complexes, and this leads to a marked increase in the phosphorylation of acetyl-CoA carboxylase. [1] Rosiglitazone activates PPAR-gamma2 which acts as a dominant inhibitor of osteoblastogenesis in murine bone marrow in vitro.[2] Rosiglitazone increases adiponectin secretion from omental cells up to 2.3-fold higher, whereas secretion from subcutaneous adipose cells is unaffected. [3] Rosiglitazone changes the morphological features and protein profiles of mitochondria in 3T3-L1 adipocytes. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 M2C3TGZ2dmO2aX;uJIF{e2G7 MV7F[oZm[3SrdnWgZ49v[2WwdILheIlwdiCjZ3HpcpN1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSHXwS|Ih[2WubIOsJGVEPTB;MD6wNkDPxE1? M3vIW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OECxPFE4Lz5zNUiwNVgyPzxxYU6=
CV-1 M1XYUmZ2dmO2aX;uJIF{e2G7 MXvJckB3cXS{bzD0doFve2O{aYD0bY9v[WxiYXP0bZZifGmxbjDv[kBR\XKxeHnzc41mKHC{b3zp[oVz[XSxcjDhZ5RqfmG2ZXSgdoVk\XC2b4Kg[4FudWFiKGDQRXIqKGW6cILld5Nm\CCrbjDDWk0yKGOnbHzzMEBGSzVyIE2gNE4xPiEQvF2u MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85PTd4OUC3K|45PTd4OUC3QE9iRg>?
CV-1 MXPGeY5kfGmxbjDhd5NigQ>? NGPiWVZC[3SrdnH0bY9vKG:oIIDldo95cXOxbXWgdJJwdGmoZYLheI9zKGGldHn2ZZRm\CC{ZXPldJRweiCpYX3tZUBu\WG|dYLl[EBjgSCrbnT1Z5Rqd25ib3[gOVAmKG:oIH3hfIlufW1iYXzrZYxqdmVicHjvd5Bp[XSjc3WgZYN1cX[rdImsJJRz[W6|ZnXjeIlwdiCjc4PhfUBqdiCFVj2xJINmdGy|LDDFR|UxKD1iMD6wPFkyOyEQvF2u MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86QDN4NkKwK|46QDN4NkKwQE9iRg>?
3T3-L1 MnXxSpVv[3Srb36gZZN{[Xl? M2jqZnN1cW23bHH0bY9vKG:oIHHkbZBw\2WwZYPpd{BqdiB|VEOtUFEh[2WubIOgbZMh\XiycnXzd4VlKGG|IHPvcoNmdnS{YYTpc44h\XG3aY\hcIVvfCC2bzD0bIUhYyBzLUG0R30hfXC2YXvlJINwfW62czDh[pRmeiC2cnXheI1mdnRid3n0bEAxNjJidXevcWwhfHKxZ3zpeIF7d26nLDDFR{A:KDBwMEmg{txONg>? M3X6VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyOUW2NlE{Lz5zMEm1OlIyOzxxYU6=
CV-1 Mo\XSpVv[3Srb36gZZN{[Xl? NXHzOYlmVWG6aX3hcEBz\XCxcoTldkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDldo95cXOxbXWgdJJwdGmoZYLheI9zKGGldHn2ZZRm\CC{ZXPldJRweiCpYX3tZUBI[Wx2IHPobY1memmlIHnuJJRz[W6|aXXueIx6KHS{YX7z[oVkfGWmIFPWMVEh[2WubIOgZpkh\nWwY4Tpc45idCCjc4PhfU4tKEWFNUCgQUAxNjB2MzFOwG0v MlmzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5MkC4OVQoRjFzN{KwPFU1RC:jPh?=
COS1 NGf4VGhHfW6ldHnvckBie3OjeR?= NEnMVGxC\2:waYP0bYMh[WO2aY\peJkhf2G|IHTleIVzdWmwZXSgbY4hS0:VMTDj[YxteyC2cnHud4Zm[3SnZDD3bZRpKEeDTDC0MXBRSVJiZ3HtcYEhemWlZYD0c5ItKEWFNUCgQUAxNjB{MTFOwG0v MmTrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ4MUe5NlQoRjF{NkG3PVI1RC:jPh?=
COS1 M2XDRWZ2dmO2aX;uJIF{e2G7 NF7OdpRKdiC4aYTyc{Bi\2:waYP0JIFkfGm4aYT5JJRme3SnZDDmc5IhfHKjboPhZ5RqfmG2aX;uJIlvKGi3bXHuJHBRSVJiZ3HtcYEuT2GuNDDjbIlu\XKrYzDDU3MuOSClZXzsd{whTUN3MDC9JFAvODJizszNMi=> NFvMVWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkeyPVY3QCd-MUK3Nlk3Pjh:L3G+
COS1 NV;CV3N5TnWwY4Tpc44h[XO|YYm= M3fpRmlvKH[rdILvJJRz[W6|YXP0bZZifGmxbjDv[kBpfW2jbjDw[ZJwgGm|b33lJJBzd2yrZnXyZZRweiCjY4TpeoF1\WRicnXj[ZB1d3JiZ3HtcYEhdWWjc4Xy[YQhcW5iUGDBVk1ISUx2IHPobY1memmlIFPPV{0yKGOnbHzzMEBGSzVyIE2gNE4xOiEQvF2u NWThfXpbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK4O|M2OTdpPkGyPFc{PTF5PD;hQi=>
CV-1 NHvUT|ZHfW6ldHnvckBie3OjeR?= MUfJckB3cXS{bzDleoFtfWG2aX;uJIFo[Wmwc4SgVnhTNWGucHjhM3BRSVJvZ3HtcYEhcW5iQ2[tNUBk\WyuczDifUBkd3S{YX7z[oVkfGmxbjDhd5NigSC5YYOg[IV1\XKvaX7l[EwhTUN3MDC9JFAvOzJ3IN88UU4> MnHrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ7NUSwOlEoRjF{OUW0NFYyRC:jPh?=
3T3-L1 M2jDS2Z2dmO2aX;uJIF{e2G7 NHLZelNG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDmc5Ih\W6qYX7j[Y1mdnRib3[gbY5{fWyrbj3pcoR2[2WmIITybYdtgWOncnnk[UBi[2O3bYXsZZRqd25iaX6gN3Q{NUxzIHPlcIx{NCCHQ{OwJF0hOC5yMjFOwG0v MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDVyNU[0OEc,OTR3MEW2OFQ9N2F-
HepG2 NE\2ZoxHfW6ldHnvckBie3OjeR?= MUjF[oZm[3SrdnWgZ49v[2WwdILheIlwdiCjZ3HpcpN1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSHXwS|Ih[2WubIOsJGVEPTBiPTCwMlA{QSEQvF2u MmS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZzMEexOVAoRjF4MUC3NVUxRC:jPh?=
U2OS M2jsNmZ2dmO2aX;uJIF{e2G7 MonzSYZn\WO2IH;uJHBRSVKpYX3tZUB1emGwc3HjeIl3[XSrb36gZYN1cX[rdImgbY4hXTKRUzDj[YxteyxiRVO1NEA:KDBwMEOg{txONg>? NXrvSZBQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[zNFA6PDRpPkG2N|AxQTR2PD;hQi=>
Huh7 M3;VNWZ2dmO2aX;uJIF{e2G7 NVHMVY5KTnWwY4Tpc45idCCjY4Tpeol1gSCjdDDoeY1idiCSUFHSJIdidW2jIHnuJGh2cDdiY3XscJMh[nlidILhcpNi[3SrdnH0bY9vKGG|c3H5MEBGSzVyIE2gNE4zOiEQvF2u NYL6c5A6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[zOlY3ODFpPkG2N|Y3PjBzPD;hQi=>
Huh7 M3izVGZ2dmO2aX;uJIF{e2G7 Mo\WSYZn\WO2IH;uJHBRSVJiZ3HtcYEhfHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JIlvKEi3aEegZ4VtdHNuIFXDOVAhRSByLkKyJO69VS5? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjR3MUC4O{c,OTZ2NUGwPFc9N2F-
HEK293 NXjFWmlSTnWwY4Tpc44h[XO|YYm= NGnEVolC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBz\WOncITvdkBqdnSncnHjeIlwdiC5aYToJJN1\XKxaXSgdoVk\XC2b4KgZ49i[3SrdnH0c5IuOSCkeTDFXWZRKGKjc3XkJJJmeG:{dHXyJIdmdmViYYPzZZk> M4fFbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NkiwNVU6Lz5zNk[4NFE2QTxxYU6=
HEK293 M2L5S2Z2dmO2aX;uJIF{e2G7 M{HOU2Fod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJJJm[2WydH;yJIlvfGW{YXP0bY9vKHerdHigdoV1cW6xaXSgXE1z\WOncITvdkBidHCqYTDifUBGYU[SIHLhd4VlKHKncH;yeIVzKGenbnWgZZN{[Xl? Mn7QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
CV1 MVfGeY5kfGmxbjDhd5NigQ>? MYHUdoFve2GldHn2ZZRqd25ib3[gVHBCWmejbX3hJIlvKEOYMTDj[YxteyxiRVO1NEA:KDBwMEe2JO69VS5? NVLUXpdtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[4NlE4PjlpPkG2PFIyPzZ7PD;hQi=>
NIH3T3 Ml;jSpVv[3Srb36gZZN{[Xl? NGC2N|NC[3Srdnn0fUBifCCqdX3hckBRWEGUIHfhcY1iKHS{YX7z[oVkfGWmIHnuJG5KUDOWMzDj[YxteyCkeTDseYNq\mW{YYPlJIFkfGm4aYT5JIF{e2G7LDDFR|UxKD1iMD6zNkDPxE1w MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjh3NEC4OUc,OTZ6NUSwPFU9N2F-
CV1 NGjnT|dHfW6ldHnvckBie3OjeR?= MXnUdoFve2GldHn2ZZRqd25ib3[gbJVu[W5iUGDBVodidW2jIHnuJGNXOSClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTBiPTCwMlMxQCEQvF2u NILldlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkm3NFM6OSd-MU[5O|A{QTF:L3G+
HepG2 NXGyUGJkTnWwY4Tpc44h[XO|YYm= MoLURYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFTKGejbX3hJIlvKGFiSHXwS|Ih[2WubIOgZpkhWFCDUj3HRWw1KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNVU5KM7:TT6= NIXqV|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkm3N|M2QCd-MU[5O|M{PTh:L3G+
HepG2 NEX0emVHfW6ldHnvckBie3OjeR?= NHPYVVJC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVJiZ3HtcYEhcW5iSHXwS|Ih[2WubIOgZpkhWFCDUj3HRWw1KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNVU5KM7:TT6= NX70UXNvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5O|k{PDFpPkG2PVc6OzRzPD;hQi=>
CV1 MVLGeY5kfGmxbjDhd5NigQ>? M4P1cGFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBqdiCFVkGgZ4VtdHNuIFXDOVAhRSByLkOwPEDPxE1w Mn\rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdyMEWzPVQoRjF5MEC1N|k1RC:jPh?=
Huh7 NETSW4dHfW6ldHnvckBie3OjeR?= Ml7GRYN1cX[rdImgZZQhcHWvYX6gVHBCWiCpYX3tZUBqdiCKdXi3JINmdGy|IHL5JJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMkKg{txONg>? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzB|NEG0PUc,OTdyM{SxOFk9N2F-
CV1 NEe1O4NHfW6ldHnvckBie3OjeR?= M3;6TWFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIFPWNUBk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkOwPEDPxE1w NXPzfVFpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexNlk4OjVpPkG3NVI6PzJ3PD;hQi=>
HepG2 M4TJVWZ2dmO2aX;uJIF{e2G7 NF3IOplC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBqdiCKZYDHNkBk\WyuczDifUBRWEGULVfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6wPFIh|ryPLh?= NIr3e3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G1O|AyQSd-MUexOVcxOTl:L3G+
HEK293 M{HIb2Z2dmO2aX;uJIF{e2G7 MVPB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIJ6KEeDTESgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yM{Wg{txONg>? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzJ7Mk[wOkc,OTd{OUK2NFY9N2F-
HEK293 Mmn6SpVv[3Srb36gZZN{[Xl? NWPBWYZJSWO2aY\peJkh[XRiaIXtZY4h[WSrcH;z[UB1cXO|dXWgVHBCWiCpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIJ6KFCSQWKtS2FNPCC2cnHud4FkfGm4YYTpc44h[XO|YYmsJGVEPTBiPTCwMlMyKM7:TT6= M2fUOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{SzN|cyLz5zN{O0N|M4OTxxYU6=
L6 NUf5ZYgzTnWwY4Tpc44h[XO|YYm= NVPB[ppjTW[oZXP0JI9vKG[jdIT5JIFkcWRib4jp[IF1cW:wIHnuJJJifCCONjDj[YxteyxiRVO1NEA:KDVizszNMi=> MofHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd|NEOzO|EoRjF5M{SzN|cyRC:jPh?=
HepG2/C3A NFrOdZJHfW6ldHnvckBie3OjeR?= MYnBZ5RqfmG2aX;uJI9nKGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5vbXXkbYF1\WRidILhcpNkemmydHnvcoFtKGGldHn2bZR6KGmwIHj1cYFvKEincFeyM2M{SSClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKG[3c3XkJGdidDRvTFLEJIJ6KGOxdILhcpNn\WO2aX;uJIF{e2G7LDDFR|UxKD1iMD6wNFM5KM7:TT6= MkTiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MEO2PFgoRjF5NECzOlg5RC:jPh?=
CV1 NH;oV5dHfW6ldHnvckBie3OjeR?= NI[0[FEzPCCqcoO= MofjRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgbY4hS1ZzIHPlcIx{KGK7IFfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7IHHmeIVzKDJ2IHjyd{whTUN3MDC9JFAvODNizszNMi=> M{PrUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUC3NlI2Lz5zN{WwO|IzPTxxYU6=
CV1 NF\vS4tHfW6ldHnvckBie3OjeR?= MYiyOEBpenN? NV65bW82SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUYXzwbIEhcW5iQ2[xJINmdGy|IHL5JGdCVDRidILhcpNi[3SrdnH0bY9vKGG|c3H5JIFnfGW{IEK0JIhzeyxiRVO1NEA:KDNwNE[g{txONg>? NUHteZJ{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NFczOjVpPkG3OVA4OjJ3PD;hQi=>
NIH3T3 NE[5PIVHfW6ldHnvckBie3OjeR?= MU\B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO|ZXSgbY4hVkmKM2SzJINmdGy|IHL5JGdCVDRidILhcpNi[3SrdnH0bY9vKGG|c3H5MEBGSzVyIE2gNE4{OiEQvF2u MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ{NEe3O{c,OTd4MkS3O|c9N2F-
HEK293 MWjGeY5kfGmxbjDhd5NigQ>? NGqwW21C\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGG|c3Xzd4VlKGG|IHHQNkBo\W6nIHnu[JVkfGmxbjygSWM2OCB;IECuNVIh|ryPLh?= NFvMO3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOECyPVE4Pid-MUiwNlkyPzZ:L3G+
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HepG2 NWr0W|VVTnWwY4Tpc44h[XO|YYm= MXOxNEB2dW:uL1y= M3PGb|I1KGi{cx?= M3W2[2lv[3KnYYPlJIlvKGeudXPvd4Uh[2:wc4XtdJRqd25iaX6gbJVu[W5iSHXwS|Ih[2WubIOgZZQhOTBidX3vcE9NKGGodHXyJFI1KGi{czDy[YxifGm4ZTD0c{Bkd262cn;s M2fCUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkW2O|Q5Lz5{MUK1Olc1QDxxYU6=
HepG2 NGfRVJFHfW6ldHnvckBie3OjeR?= MmXVWJJidnOjY4TpeoF1cW:wIH;mJIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO|ZXSgbY4hcHWvYX6gTIVxTzJiY3XscJMh[29vdILhcpNn\WO2ZXSge4l1cCCSUGLFN{1VUy2OdXOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxKD1iMD6wOUDPxE1w MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR3MES2PEc,OjF2NUC0Olg9N2F-
HepG2 MYjGeY5kfGmxbjDhd5NigQ>? MojFRYdwdmm|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIHj1cYFvKEincFeyJINmdGy|IHL5JHBRWkVvbIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4xOSEQvF2u M3rpPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEiyOFQ3Lz5{MUS4NlQ1PjxxYU6=
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3T3L1 NXm2PHl1TnWwY4Tpc44h[XO|YYm= M2fSXlExKHWP NHfQN4RKdmO{ZXHz[UBqdiCjZHnwc45m[3SrbjDtVm5CKGyndnXsd{BqdiCWTl\hcJBp[S2rbnT1Z4VlKG2xdYPlJFNVO0xzIHPlcIx{KHC{ZYTy[YF1\WRiYYSgNVAhfU1iYnXmc5JmKFSQRj3hcJBp[SClaHHscIVv\2VicnXsZZRqfmVidH:gZ49vfHKxbB?= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh3MUC4PUc,OjF6NUGwPFk9N2F-
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COS-1 NHr4RYxHfW6ldHnvckBie3OjeR?= MUKyOEBpenN? MXjB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIFPPV{0yKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmVvYnHz[YQhdHWvaX7vcYV1emmlIHHuZYx6e2m|LDDFR|UxKD1iMD6wOFgh|ryPLh?= M1HrdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEWxNFU1Lz5{MkC1NVA2PDxxYU6=
COS1 NHOyeppHfW6ldHnvckBie3OjeR?= M3LrVGJqdmSrbnegZYZncW6rdImgeI8hcHWvYX6ge4lt\CC2eYDlJHBRSVKpYX3tZUBNSkRiZYjwdoV{e2WmIHnuJGNQWzFiY3XscJMh[29vZYjwdoV{e2mwZzDHRWw1KGK7IIPjbY51cWyuYYTpc44heHKxeHntbZR6KGG|c3H5 M3zOVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEewOlA1Lz5{MkC3NFYxPDxxYU6=
HepG2 M4\zVmZ2dmO2aX;uJIF{e2G7 MWKyNEBpenN? MmOyRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDHRWw1NW[3c3XkJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJGhmeEd{IHPlcIx{KGGodHXyJFIxKGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMjFOwG0v NGrpbHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC4NVk{Oid-MkKwPFE6OzJ:L3G+
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COS1 NXXaZ24{TnWwY4Tpc44h[XO|YYm= NWD2T|ZpOjRiaILz NFTRUoVVemGwc3HjeIl3[XSrb36gc4YhcHWvYX6g[pVtdCCuZX7neIghWFCDUnfhcY1iKGW6cILld5Nm\CCrbjDDU3MyKGOnbHzzJINwNXS{YX7z[oVkfGWmIIfpeIghWliUYXzwbIEh[W[2ZYKgNlQhcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMUKg{txONg>? MlXIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzOUezPVYoRjJ{MUm3N|k3RC:jPh?=
MG-63 NWDvZ4Q1TnWwY4Tpc44h[XO|YYm= NGDpOWlC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iTVetOlMh[2WubIOgZpkhemWyb4L0[ZIh\2WwZT3iZZNm\CC2cnHud4FkfGm4YYTpc44h[XO|YYmsJGVEPTBiPTCwMlAyOSEQvF2u NWrINVVJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyNlU3PDFpPkKyNlI2PjRzPD;hQi=>
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HepG2 MVXGeY5kfGmxbjDhd5NigQ>? MnT4WJJidnOjY4TpeoF1cW:wIH;mJGdCVDRvZoXz[YQhWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjD0doFve2[nY4Tl[EBqdiCqdX3hckBJ\XCJMjDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjFizszNMi=> NGW1T5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmzOFU{Pyd-MkK5N|Q2Ozd:L3G+
THP1 Ml;QSpVv[3Srb36gZZN{[Xl? M{TETlExOCC3TR?= NUn6N29xOyCqcoO= M2Ljd3Rz[W6|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5iaIXtZY4hXEiSMTDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckBNWFNvaX7keYNm\CCPQ2CxJI1TVkFiZYjwdoV{e2mxbjDheEAyODBidV2gdJJmcW6ldXLheIVlKG[xcjCzJIhzeyCycnnvdkB1dyCOUGOgZ4hidGynbnflJI1m[XO3cnXkJIFnfGW{IEG4JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXNicnXsZZRqfmVidH:geY51emWjdHXkJINwdnS{b3y= M1rSV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUO0OVM4Lz5{MkmzOFU{PzxxYU6=
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HepG2 M1nR[2Z2dmO2aX;uJIF{e2G7 Mn;HNVAhfU1? MV:yOEBpenN? MWLS[YR2[3Srb36gc4Yh\2y3Y3;z[UBkd26|dX3weIlwdiCrbjDpcpN2dGmwLYLld4l{fGGwdDDoeY1idiCKZYDHNkBk\WyuczDheEAyOCC3TTDh[pRmeiB{NDDodpMh[nliZ3z1Z49{\SCxeHnkZZNmKG2ndHjv[EBqdiCycnXz[Y5k\SCxZjCwMlEhfU1ib3[gbY5{fWyrbh?= NYrJW2g{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNlUzPDRpPkKzNFI2OjR2PD;hQi=>
HepG2 M2\BbmZ2dmO2aX;uJIF{e2G7 MmDlNVAhfU1? NWeydWROOjRiaILz NV3XfoxLWmWmdXP0bY9vKG:oIHfseYNwe2ViY3;ud5VueHSrb36gbY4hcW6|dXzpck1z\XOrc4ThcpQhcHWvYX6gTIVxTzJiY3XscJMh[XRiMUCgeW0h[W[2ZYKgNlQhcHK|IHL5JIdtfWOxc3Wgc5hq\GG|ZTDt[ZRpd2RiaX6gdJJme2WwY3Wgc4YhOjJwMjDtUUBw\iCpbIXjc5Nm M1zJRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEK1NlQ1Lz5{M{CyOVI1PDxxYU6=
HepG2 MYfGeY5kfGmxbjDhd5NigQ>? M4HpfVIxKGi{cx?= NUniPIZHXHKjboPhZ5RqfmG2aX;uJI9nKEeDTESt[pV{\WRiaIXtZY4hWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO|ZXSgbY4hUGWyR{KgZ4VtdHNiYX\0[ZIhOjBiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFM6KM7:TT6= NFuy[Gg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G3NVA1PSd-MkOxO|ExPDV:L3G+
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HepG2 M1vmcGZ2dmO2aX;uJIF{e2G7 MVfB[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRvZoXz[YQhWFCDUnfhcY1iKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIHj1cYFvKEincFeyJINmdGy|IHL5JJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEGg{txONg>? M4\MbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUCyNlEzLz5{M{WwNlIyOjxxYU6=
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3T3L1 NX74O3RiTnWwY4Tpc44h[XO|YYm= MVSxNEB2VQ>? MV[yOEBpenN? NXjzSm1WUW6mdXP0bY9vKG:oIHnud5VtcW5vc4TpcZVt[XSnZDCyMYRmd3i7LVStX|NJZS2pbIXjc5NmKHWydHHr[UBqdiCmaX\m[ZJmdnSrYYTl[EBud3W|ZTCzWFNNOSClZXzsd{BifCBzMDD1UUBi\nSncjCyOEBpenNiYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yCjbnHsfZNqew>? MmfTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVIyNyd-Q3jFUWJNRC:jPh?=
3T3L1 Mlq0SpVv[3Srb36gZZN{[Xl? M2T5ZVIhfU1? M2\QO|E1KGSjeYO= M2DaNGFod26rc4SgZYN1cX[rdImgZZQhWFCDUjDpckBud3W|ZTCzWFNNOSClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDh[Ilxd2O7dHWg[Iln\mW{ZX70bYF1cW:wIHH0JFIhfU1iYX\0[ZIhOTRiZHH5d{BjgSCRaXygVoVlKE9ic4ThbY5qdmdibXX0bI9l M4PGbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=
U2OS NV7sdG1lTnWwY4Tpc44h[XO|YYm= M4f5NFExKHWP MVOyOEBpenN? NEfSflBC[3SrdnH0bY9vKG:oIGDQRXJIKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIHj1cYFvKFV{T2OgZ4VtdHNiYYSgNVAhfU1iaX7jeYJifGWmIH\vdkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7 MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlEwLz6FaFXNRmw9N2F-
Assay
Methods Test Index PMID
Western blot Cyclin D1 / CDK2 / CDK4 / Bax / Bcl-2 29725405
Immunofluorescence PPARγ / E-cadherin 27145370
Growth inhibition assay Cell viability 29725405
In vivo

Rosiglitazone administration results in significant bone loss, including a decrease in bone volume, trabecular width, and trabecular number and an increase in trabecular spacing. Rosiglitazone also leads to a decrease in bone formation rate, with a simultaneous increase in fat content in the bone marrow. Rosiglitazone decreases the expression of the osteoblast-specific genes Runx2/Cbfa1, Dlx5, and alpha1(I)collagen, whereas the expression of the adipocyte-specific fatty acid binding protein aP2 is increased. [2] Rosiglitazone upregulates gene transcripts encoding mitochondrial proteins in white adipocytes from ob/ob mice accompanied by an increase in mitochondrial mass and changes in mitochondrial structure. [4]

Protocol (from reference)

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

5mg/mL

Chemical Information

Molecular Weight 357.43
Formula

C18H19N3O3S

CAS No. 122320-73-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CN(CCOC1=CC=C(C=C1)CC2C(=O)NC(=O)S2)C3=CC=CC=N3

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02737709 Terminated Drug: Paclitaxel and Carboplatin regimen Non-small Cell Lung Cancer Sun Yat-sen University March 2016 Phase 2
NCT01402076 Completed Drug: Tasimelteon|Drug: Rosiglitazone|Drug: Midazolam Healthy Volunteers Vanda Pharmaceuticals August 2011 Phase 1
NCT01100619 Completed Drug: rosiglitazone|Drug: XL184 Papillary Thyroid Cancer|Follicular Thyroid Cancer|Huerthle Cell Thyroid Cancer|Renal Cell Carcinoma Exelixis April 2010 Phase 1
NCT01415128 Completed Drug: Omeprazole|Drug: Rosiglitazone|Drug: Desipramine Erectile Dysfunction VIVUS LLC April 2010 Phase 1
NCT01376063 Completed Drug: FG-4592 Healthy Adult Subjects FibroGen March 2010 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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