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Rosiglitazone (BRL 49653)

Name: Rosiglitazone (BRL 49653)
Brand: Selleck Chemicals
SKU: S2556
Rating: 5 (46 reviews)

Catalog No.S2556

For research use only.

Rosiglitazone (BRL 49653) is a potent antihyperglycemic agent and a potent thiazolidinedione insulin sensitizer with IC50 of 12, 4 and 9 nM for rat, 3T3-L1 and human adipocytes, respectively. Rosiglitazone is a pure ligand of PPAR-gamma, and has no PPAR-alpha-binding action. Rosiglitazone modulates TRP channels and induces autophagy. Rosiglitazone prevents ferroptosis.

Rosiglitazone (BRL 49653) Chemical Structure

CAS No. 122320-73-4

Selleck's Rosiglitazone (BRL 49653) has been cited by 46 publications

Purity & Quality Control

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PPAR Signaling Pathway Map

Biological Activity

Description

Targets

PPAR ^[1]
(Cell-free assay)

TRPC ^[5]
()

Ferroptosis ^[6]
()

In vitro

Rosiglitazone markedly increase phosphorylation of threonine 172 within the α subunit of AMPK, with increased AMP:ATP ratio. Rosiglitazone has been reported to decrease cholesterol synthesis in a number of cell lines in a peroxisome proliferator-activated receptor γ-independent manner. Rosiglitazone activates both α1- and α2-containing AMPK complexes, and this leads to a marked increase in the phosphorylation of acetyl-CoA carboxylase. ^[1] Rosiglitazone activates PPAR-gamma2 which acts as a dominant inhibitor of osteoblastogenesis in murine bone marrow in vitro.^[2] Rosiglitazone increases adiponectin secretion from omental cells up to 2.3-fold higher, whereas secretion from subcutaneous adipose cells is unaffected. ^[3] Rosiglitazone changes the morphological features and protein profiles of mitochondria in 3T3-L1 adipocytes. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

HepG2	M4jLSWZ2dmO2aX;uJIF{e2G7			MoPPSYZn\WO2aY\lJINwdmOnboTyZZRqd25iYXfhbY5{fCCqdX3hckBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjldGczKGOnbHzzMEBGSzVyPUCuNFIh\|ryP	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPThyMUixO{c,OTV6MEG4NVc9N2F-
CV-1	MWrGeY5kfGmxbjDhd5NigQ>?			NInlfHdKdiC4aYTyc{B1emGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBw\iCSZYLvfIl{d22nIIDyc4xq\mW{YYTvdkBi[3SrdnH0[YQhemWlZYD0c5Ih\2GvbXGgLHBRSVJrIHX4dJJme3OnZDDpckBEXi1zIHPlcIx{NCCHQ{WwJF0hOC5yNjFOwG0v	M4e0T\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzh3N{[5NFcoRjh3N{[5NFc9N2F-
CV-1	MXTGeY5kfGmxbjDhd5NigQ>?			NGmzfWhC[3SrdnH0bY9vKG:oIIDldo95cXOxbXWgdJJwdGmoZYLheI9zKGGldHn2ZZRm\CC{ZXPldJRweiCpYX3tZUBu\WG\|dYLl[EBjgSCrbnT1Z5Rqd25ib3[gOVAmKG:oIH3hfIlufW1iYXzrZYxqdmVicHjvd5Bp[XSjc3WgZYN1cX[rdImsJJRz[W6\|ZnXjeIlwdiCjc4PhfUBqdiCFVj2xJINmdGy\|LDDFR\|UxKD1iMD6wPFkyOyEQvF2u	MlfSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTh\|Nk[yNEc,QTh\|Nk[yNFww[T5?
3T3-L1	MlLuSpVv[3Srb36gZZN{[Xl?			NWG3[3dnW3SrbYXsZZRqd25ib3[gZYRqeG:pZX7ld4l{KGmwIEPUN{1NOSClZXzsd{BqeyCneIDy[ZN{\WRiYYOgZ49v[2WwdILheIlwdiCncYXpeoFt\W62IITvJJRp\SCdIEGtNVREZSC3cIThb4Uh[2:3boTzJIFnfGW{IITy[YF1dWWwdDD3bZRpKDBwMjD1[{9uVCC2cn;ncIl1[XqxbnWsJGVEKD1iMD6wPUDPxE1w	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODl3NkKxN{c,OTB7NU[yNVM9N2F-
CV-1	NYG0S4xwTnWwY4Tpc44h[XO\|YYm=			M3HyN21igGmvYXygdoVxd3K2ZYKgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCSZYLvfIl{d22nIIDyc4xq\mW{YYTvdkBi[3SrdnH0[YQhemWlZYD0c5Ih\2GvbXGgS4FtPCClaHnt[ZJq[yCrbjD0doFve2mnboTsfUB1emGwc3\lZ5Rm\CCFVj2xJINmdGy\|IHL5JIZ2dmO2aX;uZYwh[XO\|YYmuMEBGSzVyIE2gNE4xPDNizszNMi=>	Mo\zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5MkC4OVQoRjFzN{KwPFU1RC:jPh?=
COS1	MYDGeY5kfGmxbjDhd5NigQ>?			Ml23RYdwdmm\|dHnjJIFkfGm4aYT5JJdieyCmZYTldo1qdmWmIHnuJGNQWzFiY3XscJMhfHKjboPm[YN1\WRid3n0bEBISUxiND3QVGFTKGejbX3hJJJm[2WydH;yMEBGSzVyIE2gNE4xOjFizszNMi=>	M{fqXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{NkG3PVI1Lz5zMk[xO\|kzPDxxYU6=
COS1	MmOzSpVv[3Srb36gZZN{[Xl?			NV\PZ3d6UW5idnn0do8h[WexbnnzeEBi[3Srdnn0fUB1\XO2ZXSg[o9zKHS{YX7zZYN1cX[jdHnvckBqdiCqdX3hckBRWEGUIHfhcY1iNUejbESgZ4hqdWW{aXOgR29UNTFiY3XscJMtKEWFNUCgQUAxNjB{IN88UU4>	M3LnZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{N{K5OlY5Lz5zMkeyPVY3QDxxYU6=
COS1	NV:wOGhITnWwY4Tpc44h[XO\|YYm=			NWDkdVBqUW5idnn0do8hfHKjboPhZ5RqfmG2aX;uJI9nKGi3bXHuJJBmem:6aYPvcYUheHKxbHnm[ZJifG:{IHHjeIl3[XSnZDDy[YNmeHSxcjDnZY1u[SCvZXHzeZJm\CCrbjDQVGFTNUeDTESgZ4hqdWW{aXOgR29UNTFiY3XscJMtKEWFNUCgQUAxNjB{IN88UU4>	NGPj[lk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMki3N\|UyPyd-MUK4O\|M2OTd:L3G+
CV-1	Mon5SpVv[3Srb36gZZN{[Xl?			NGe2dplKdiC4aYTyc{BmfmGudXH0bY9vKGGpYXnud5QhWliULXHsdIhiN1CSQWKt[4FudWFiaX6gR3YuOSClZXzsd{BjgSClb4TyZY5{\mWldHnvckBie3OjeTD3ZZMh\GW2ZYLtbY5m\CxiRVO1NEA:KDBwM{K1JO69VS5?	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjl3NEC2NUc,OTJ7NUSwOlE9N2F-
3T3-L1	MUXGeY5kfGmxbjDhd5NigQ>?			M33NT2Vn\mWldHn2[UBkd26lZX70doF1cW:wIH\vdkBmdmijbnPlcYVvfCCxZjDpcpN2dGmwLXnu[JVk\WRidILp[4x6[2W{aXTlJIFk[3WvdXzheIlwdiCrbjCzWFMuVDFiY3XscJMtKEWFM{CgQUAxNjB{IN88UU4>	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDVyNU[0OEc,OTR3MEW2OFQ9N2F-
HepG2	M4HpdGZ2dmO2aX;uJIF{e2G7			MWTF[oZm[3SrdnWgZ49v[2WwdILheIlwdiCjZ3HpcpN1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSHXwS\|Ih[2WubIOsJGVEPTBiPTCwMlA{QSEQvF2u	NHO3U3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkGwO\|E2OCd-MU[xNFcyPTB:L3G+
U2OS	NXiwc5hITnWwY4Tpc44h[XO\|YYm=			MVvF[oZm[3Rib36gVHBCWmejbX3hJJRz[W6\|YXP0bZZifGmxbjDhZ5Rqfmm2eTDpckBWOk:VIHPlcIx{NCCHQ{WwJF0hOC5yMzFOwG0v	NVPX[oh{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[zNFA6PDRpPkG2N\|AxQTR2PD;hQi=>
Huh7	NGjITIZHfW6ldHnvckBie3OjeR?=			M2jROGZ2dmO2aX;uZYwh[WO2aY\peJkh[XRiaIXtZY4hWFCDUjDnZY1u[SCrbjDIeYg4KGOnbHzzJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNlIh\|ryPLh?=	NGXveXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkO2OlYxOSd-MU[zOlY3ODF:L3G+
Huh7	MX\GeY5kfGmxbjDhd5NigQ>?			NVn1OmJETW[oZXP0JI9vKFCSQWKg[4FudWFidILhcpNi[3SrdnH0bY9vKGGldHn2bZR6KGmwIFj1bFch[2WubIOsJGVEPTBiPTCwMlIzKM7:TT6=	M3vjZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NEWxNFg4Lz5zNkS1NVA5PzxxYU6=
HEK293	MY\GeY5kfGmxbjDhd5NigQ>?			MkS3RYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gdoVk\XC2b4KgbY51\XKjY4Tpc44hf2m2aDDzeIVzd2mmIILlZ4VxfG:{IHPvZYN1cX[jdH;yMVEh[nliRWnGVEBj[XOnZDDy[ZBwenSncjDn[Y5mKGG\|c3H5	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
HEK293	MUPGeY5kfGmxbjDhd5NigQ>?			NYrO[pNVSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhemWlZYD0c5IhcW62ZYLhZ5Rqd25id3n0bEBz\XSrbn;p[EBZNXKnY3XweI9zKGGucHjhJIJ6KEW\RmCgZoF{\WRicnXwc5J1\XJiZ3Xu[UBie3OjeR?=	Moe2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
CV1	MoO0SpVv[3Srb36gZZN{[Xl?			MYfUdoFve2GldHn2ZZRqd25ib3[gVHBCWmejbX3hJIlvKEOYMTDj[YxteyxiRVO1NEA:KDBwMEe2JO69VS5?	M1PXTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OEKxO\|Y6Lz5zNkiyNVc3QTxxYU6=
NIH3T3	MlflSpVv[3Srb36gZZN{[Xl?			MWjBZ5Rqfmm2eTDheEBpfW2jbjDQVGFTKGejbX3hJJRz[W6\|ZnXjeIVlKGmwIF7JTFNVOyClZXzsd{BjgSCudXPp[oVz[XOnIHHjeIl3cXS7IHHzd4F6NCCHQ{WwJF0hOC5\|MjFOwG0v	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjh3NEC4OUc,OTZ6NUSwPFU9N2F-
CV1	MX3GeY5kfGmxbjDhd5NigQ>?			MknmWJJidnOjY4TpeoF1cW:wIH;mJIh2dWGwIGDQRXJo[W2vYTDpckBEXjFiY3XscJMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4{ODhizszNMi=>	M{DwVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUewN\|kyLz5zNkm3NFM6OTxxYU6=
HepG2	NYHJZ\|RRTnWwY4Tpc44h[XO\|YYm=			MVrB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXIh\2GvbXGgbY4h[SCKZYDHNkBk\WyuczDifUBRWEGULVfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6xOVgh\|ryPLh?=	M37sVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUezN\|U5Lz5zNkm3N\|M2QDxxYU6=
HepG2	M4C5bGZ2dmO2aX;uJIF{e2G7			M4S0Z2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWiCpYX3tZUBqdiCKZYDHNkBk\WyuczDifUBRWEGULVfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6xOVgh\|ryPLh?=	NX65PW1RRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5O\|k{PDFpPkG2PVc6OzRzPD;hQi=>
CV1	M1HGU2Z2dmO2aX;uJIF{e2G7			NF[yVJlC[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEhcW5iQ2[xJINmdGy\|LDDFR\|UxKD1iMD6zNFgh\|ryPLh?=	M1\STlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MEC1N\|k1Lz5zN{CwOVM6PDxxYU6=
Huh7	NHyw[JpHfW6ldHnvckBie3OjeR?=			MUTBZ5Rqfmm2eTDheEBpfW2jbjDQVGFTKGejbX3hJIlvKEi3aEegZ4VtdHNiYomgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5{MjFOwG0v	NVW1NHhlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUewN\|QyPDlpPkG3NFM1OTR7PD;hQi=>
CV1	NF[wS25HfW6ldHnvckBie3OjeR?=			MXHB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCFVkGgZ4VtdHNiYomgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5\|MEig{txONg>?	NITPeIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{GyPVczPSd-MUexNlk4OjV:L3G+
HepG2	NXL0Z4hbTnWwY4Tpc44h[XO\|YYm=			M1OwTGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIlvKEincFeyJINmdGy\|IHL5JHBRSVJvR1HMOEB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkC4NkDPxE1w	MorZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzNUewNVkoRjF5MUW3NFE6RC:jPh?=
HEK293	Mkn3SpVv[3Srb36gZZN{[Xl?			MmfjRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{BjgSCJQVy0JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEO1JO69VS5?	NHG0ZnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{K5NlYxPid-MUeyPVI3ODZ:L3G+
HEK293	NWDKUGpFTnWwY4Tpc44h[XO\|YYm=			MUHBZ5Rqfmm2eTDheEBpfW2jbjDh[Ilxd3OnIITpd5N2\SCSUFHSJIdidW2jIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYomgVHBCWi2JQVy0JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwM{Gg{txONg>?	MlG0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd\|NEOzO\|EoRjF5M{SzN\|cyRC:jPh?=
L6	MWnGeY5kfGmxbjDhd5NigQ>?			MXrF[oZm[3Rib36g[oF1fHliYXPp[EBwgGmmYYTpc44hcW5icnH0JGw3KGOnbHzzMEBGSzVyIE2gOUDPxE1w	NIT5U3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{O0N\|M4OSd-MUezOFM{PzF:L3G+
HepG2/C3A	NE[xNpdHfW6ldHnvckBie3OjeR?=			NGeyNIVC[3SrdnH0bY9vKG:oIHj1cYFvKFCSQWLnZY1u[SCuaXfhcoQh[mmwZHnu[{Bld22jaX6tcYVlcWG2ZXSgeJJidnOlcnnweIlwdmGuIHHjeIl3cXS7IHnuJIh2dWGwIFjldGczN0N\|QTDj[YxteyClbz30doFve2[nY4Tl[EB4cXSqIH\1d4VlKEejbEStUGJFKGK7IHPveJJidnOoZXP0bY9vKGG\|c3H5MEBGSzVyIE2gNE4xODN6IN88UU4>	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRyM{[4PEc,OTd2MEO2PFg9N2F-
CV1	NYLpXGY{TnWwY4Tpc44h[XO\|YYm=		NVuwPJM{OjRiaILz	MlzhRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgbY4hS1ZzIHPlcIx{KGK7IFfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7IHHmeIVzKDJ2IHjyd{whTUN3MDC9JFAvODNizszNMi=>	NXTZWXpvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NFczOjVpPkG3OVA4OjJ3PD;hQi=>
CV1	NGTCWXZHfW6ldHnvckBie3OjeR?=		NUnNRohNOjRiaILz	NELrN2tC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKjbIDoZUBqdiCFVkGgZ4VtdHNiYomgS2FNPCC2cnHud4FkfGm4YYTpc44h[XO\|YYmgZYZ1\XJiMkSgbJJ{NCCHQ{WwJF0hOy52NjFOwG0v	NHTBcoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WwO\|IzPSd-MUe1NFczOjV:L3G+
NIH3T3	NW\jOIllTnWwY4Tpc44h[XO\|YYm=			NWfQPZRpSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIF7JTFNVOyClZXzsd{BjgSCJQVy0JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwM{Kg{txONg>?	MmLsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4MkS3O\|coRjF5NkK0O\|c4RC:jPh?=
HEK293	NU\ybFQ5TnWwY4Tpc44h[XO\|YYm=			NGr5c3dC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHHQNkBo\W6nIHnu[JVkfGmxbjygSWM2OCB;IECuNVIh\|ryPLh?=	NWPIRWk5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiwNlkyPzZpPkG4NFI6OTd4PD;hQi=>
HEK293	MXHGeY5kfGmxbjDhd5NigQ>?			MlSwRYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{BjgSCJQVy0JJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMUmg{txONg>?	NIfLNFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOECyPVE4Pid-MUiwNlkyPzZ:L3G+
CV1	MmfpSpVv[3Srb36gZZN{[Xl?			NVn4UYhNSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFPWNUBk\WyuczDifUBz\WOncITvdkB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkOwPEDPxE1w	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB{OUG3PEc,OThyMkmxO\|g9N2F-
HEK293	NFu2O4xHfW6ldHnvckBie3OjeR?=			MXnB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCKRVuyPVMh[2WubIOgZpkhT0GONDD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjB2NTFOwG0v	M1m4T\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkmxOlQ2Lz5zOEK5NVY1PTxxYU6=
U2OS	MVXGeY5kfGmxbjDhd5NigQ>?			MnPMRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgbY4hXTKRUzDj[YxteyCkeTD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjB{IN88UU4>	M{e4SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{K5O\|UyLz5zOEOyPVc2OTxxYU6=
HepG2	NHnKN2dHfW6ldHnvckBie3OjeR?=			NYj5eoxrSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjldGczKGOnbHzzJIJ6KEeDTESgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5{MkOg{txONg>?	NXPJe\|dIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizPVQ6ODdpPkG4N\|k1QTB5PD;hQi=>
HepG2	MnjHSpVv[3Srb36gZZN{[Xl?			NUn3R3pySWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiZYjwdoV{e2WmIHnuJIh2dWGwIFjldGczKGOnbHzzJIF{e2W\|c3XkJIF{KGmwZIXjeIlwdiCxZjDy[YNmeHSxcjD0doFve2GldHn2ZZRqd25iYomgdoVxd3K2ZYKg[4Vv\SCjc4PhfUBz\WyjdHn2[UB1dyClb370do9tNCCHQ{WwJF0hOC5yNTFOwG0v	M1fRSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkK1OVU6Lz5zOE[yOVU2QTxxYU6=
RAW264.7	NGW3N2pHfW6ldHnvckBie3OjeR?=			Mk\iV5VxeHKnc4Ppc44hd2ZiTGDTM2lHVi2pYX3tZU1{fGmvdXzheIVlKE6RIIDyc4R2[3Srb36gbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNuIFnDOVAhRSBzNz61JO69VS5?	NHS0WIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEiwPVMzPSd-MUi4NFk{OjV:L3G+
CV1	NYTmXGdxTnWwY4Tpc44h[XO\|YYm=			NW\LZ21QSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEhdGmpYX7kJIJqdmSrbneg[I9u[WmwIHX4dJJme3OnZDDpckBi\nKrY3HuJIdz\WWwIH3vcotmgSCFVkGgZ4VtdHNiY3:teJJidnOoZXP0[YQhf2m2aDDmeZNm\CCJYXy0MWRDTCCkeTD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjB\|MzFOwG0v	M2XBOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OEK2NlA2Lz5zOEiyOlIxPTxxYU6=
CV1	MVnGeY5kfGmxbjDhd5NigQ>?			MXzB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJidHCqYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIHHmdolk[W5iZ4Ll[Y4hdW:wa3X5JGNXOSClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKG[3c3XkJGdidDRvRFLEJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IEOuOFYh\|ryPLh?=	MlXHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh6Mk[yNFUoRjF6OEK2NlA2RC:jPh?=
HepG2	M2fTeWZ2dmO2aX;uJIF{e2G7			MV;B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJidHCqYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIHj1cYFvKEincFeyJINmdGy\|IHPvMZRz[W6\|ZnXjeIVlKHerdHigS4FtPCCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjB\|OTFOwG0v	M3zqWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OEO1O\|E6Lz5zOEizOVcyQTxxYU6=
HepG2	NVr3dmF5TnWwY4Tpc44h[XO\|YYm=			M4TIRmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gbJVu[W5iSHXwJGczKGOnbHzzJINwNXS{YX7z[oVkfGWmIIfpeIghT2GuND3ERmQh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4xPCEQvF2u	M4PPUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEWzO\|c3Lz5zOUC1N\|c4PjxxYU6=
HeLa	M4LkZ2Z2dmO2aX;uJIF{e2G7			M3HZTmFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO\|ZXSgbY4hcHWvYX6gTIVN[SClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKEejbEStSGJFKGG\|c3Xzd4VlKGG\|IITyZY5{[3KrcITpc45idCCjY4TpeoF1cW:wIHL5JGdidDRicnXzdI9ve2ViZXzlcYVvfC2mcnn2[Y4hdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxKD1iMD6wNVUh\|ryPLh?=	NGDBNos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUK3OVk3Oyd-MUmyO\|U6PjN:L3G+
BL21	MnHqSpVv[3Srb36gZZN{[Xl?			M3y1c2Rqe3CuYXPlcYVvfCCxZjDyZYRqdyCuYXLlcIVlKDJqUzmtLFIu[mWwen;5cE1xcGWweXzhcYlvdylvMz37OE1cOSxzLXTpeJJqfGmxLUKtLFUudWW2aInsMVIueGinbonsMY95[XqxbD20MZltMS2ndHjvfJleNXCqZX75cJ0ueHKxcHnvcolkKGGlaXSg[pJwdSCJU2St[pV{\WRiaIXtZY4hWFCDUnfhcY1iKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEh[2WubIOgZpkhe2OrboTpcIxifGmxbjDwdo95cW2rdImgZZN{[XluIFnDOVAhRSByLkS1JO69VS5?	NHjtZVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO0PVE4Pid-MUmzOFkyPzZ:L3G+
BHK21	MX7GeY5kfGmxbjDhd5NigQ>?			M1TY[2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCESFuyNUBk\WyuczDhd5Nme3OnZDDhd{BUTUGSIHHjeIl3cXS7IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD60OUDPxE1w	NGTWN5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO0PVE4Pid-MUmzOFkyPzZ:L3G+
COS1	NWm3VnNLTnWwY4Tpc44h[XO\|YYm=		NYPa[mZtPDhiaILz	NEP0UGpC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJJJm[2:vYnnuZY51KFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gR29UOSClZXzsd{Bkdy2neIDy[ZN{cW6pIFfBUFQh[XO\|ZYPz[YQh[XNidILhcpNkemmydHnvcoFtKGGldHn2bZR6KGGodHXyJFQ5KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCyJO69VS5?	NFfzWJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWwO\|g3OSd-MUm1NFc5PjF:L3G+
COS1	M4rSV2Z2dmO2aX;uJIF{e2G7			MoruRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgdoVk\XC2b4Kg[ZhxemW\|c3XkJIlvKGGocnnjZY4h\3KnZX6gcY9vc2W7IFPPV\|Eh[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBnfXOnZDD5[YF{fCCJYXy0MWRDTCCkeTD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjB{IN88UU4>	M3LYbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUOwOlgyLz5zOUWzNFY5OTxxYU6=
U2OS	MYTGeY5kfGmxbjDhd5NigQ>?			Ml7QRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKFV{T2OgZ4VtdHNiYomgcJVkcW[ncnHz[UB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkC1JO69VS5?	NF7vXHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUW3OFA2Pid-MUm1O\|QxPTZ:L3G+
MCF7	MYXGeY5kfGmxbjDhd5NigQ>?	Mn:yNE4yKHWP	NW\MNphvOjRiaILz	NGC2TZBC[3SrdnH0bY9vKG:oIHj1cYFvKFCSQWKt[4FudWFvZHXw[Y5l\W62IITyZY5{[3KrcITpc44h\XiycnXzd4VlKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKFCSUlWtdoVxd3K2ZYKg[4Vv\SCneIDy[ZN{cW:wIHH0JFAvOSC3TTDh[pRmeiB{NDDodpMh[nliZIXhcEBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XlicnXsZZRqfmVidH:gdo9{cWeuaYThfo9v\Q>?	MonWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl5MUmyN\|YoRjF7N{G5NlM3RC:jPh?=
HepG2	MlfZSpVv[3Srb36gZZN{[Xl?			NU\MenJnSWexbnnzeEBi[3Srdnn0fUBifCCJQVy0MZRi\2enZDDoeY1idiCSUFHS[4FudWFibHnnZY5lKGKrbnTpcoch\G:vYXnuJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\XCJMjDj[YxteyCjc4Pld5Nm\CCjczDy[YNmeHSxcjD0doFve2GldHn2ZZRqd25iYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yM{mg{txONg>?	NXntPWd3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm3O\|UyPjlpPkG5O\|c2OTZ7PD;hQi=>
HepG2	MUDGeY5kfGmxbjDhd5NigQ>?			NXPnOGlqSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjldGczKGOnbHzzJIJ6KEeDTESgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5{MkOg{txONg>?	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd6M{S0OEc,OTl5OEO0OFQ9N2F-
COS7	Ml7YSpVv[3Srb36gZZN{[Xl?			NXLSbFBOSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBz\WOxbXLpcoFvfCCSUFHS[4FudWFzIFzCSEBmgHC{ZYPz[YQhcW5iYX\ybYNidiCpcnXlckBud26tZYmgR29UPyClZXzsd{Bkd2W6cILld5NqdmdiR1HMOEBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6wN{DPxE1w	NIPpXmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEGzPFc3Oid-MkCxN\|g4PjJ:L3G+
COS7	NGr4S2ZHfW6ldHnvckBie3OjeR?=			NWDnUHJvSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBz\WOxbXLpcoFvfCCSUFHS[4FudWF{IFzCSEBmgHC{ZYPz[YQhcW5iYX\ybYNidiCpcnXlckBud26tZYmgR29UPyClZXzsd{Bkd2W6cILld5NqdmdiR1HMOEBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6xJO69VS5?	NIKybZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEGzPFc3Oid-MkCxN\|g4PjJ:L3G+
HEK	NGL2UmhHfW6ldHnvckBie3OjeR?=			NHnNSYlC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGdCVDRvdHHn[4VlKFCSQWLnZY1u[SClaHnt[ZJq[yC{ZXPldJRweiCneIDy[ZN{\WRiaX6gTGVMKGOnbHzzJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNFM2KM7:TT6=	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODJ7OUKxOEc,OjB{OUmyNVQ9N2F-
HEK	NHPZZphHfW6ldHnvckBie3OjeR?=			MmfuRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDHRWw1NXSjZ3fl[EBRWEGUYXzwbIEh[2irbXXybYMhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT{Bk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSBzMD61PEDPxE1w	Mnu5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB{OUmyNVQoRjJyMkm5NlE1RC:jPh?=
CHO-K1	MX7GeY5kfGmxbjDhd5NigQ>?			M4TxZnBienSrYXygZYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGtUGJFKGW6cILld5Nm\CCrbjDDTG8uUzFiY3XscJMh[29vdILhcpNn\WO2ZXSge4l1cCCJQVy0JIF{e2W\|c3XkJIF{KGy3Y3nm[ZJie2ViYXP0bZZqfHliYomgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5zIN88UU4>	M1;tSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNUK3PVY6Lz5{MEWyO\|k3QTxxYU6=
3T3L1	M1[4NmZ2dmO2aX;uJIF{e2G7		MWm3JIRigXN?	MmnPTY5lfWO2aX;uJI9nKGGmaYDv[4Vv\XOrczDpckBud3W\|ZTCzWFNNOSClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDsbZBq\CCjY3P1cZVt[XSrb36gZYZ1\XJiNzDkZZl{KGK7IF;pcEBz\WRiTzDzeIFqdmmwZx?=	NUe4bG1WRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1Nlc6PjlpPkKwOVI4QTZ7PD;hQi=>
HEK293T	NHXBWmxHfW6ldHnvckBie3OjeR?=	MUGxJJVO	M4HZN\|I1KGi{cx?=	NYLTTJFFWGG{dHnhcEBi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZU1NSkRiZYjwdoV{e2WmIHnuJGhGUzJ7M2SgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKHKnY3XweI9zKHS{YX7zZYN1cX[jdHnvckBifCBzIIXNJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk>	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB\|MEK2N{c,OjFyM{CyOlM9N2F-
CV1	M2fQNGZ2dmO2aX;uJIF{e2G7			M2HYbHRz[W6\|YXP0bZZifGmxbjDv[kBI[Wx2LX\1d4VlKGi3bXHuJHBRSVKpYX3tZUBFVkFiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIHHmdolk[W5iZ4Ll[Y4hdW:wa3X5JGNXOSClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlEh\|ryPLh?=	M2DmUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUGyO\|g1Lz5{MUGxNlc5PDxxYU6=
HEK293	M3XXTWZ2dmO2aX;uJIF{e2G7		MmPGNVYhfG9iMkCgbJJ{	MV;UdoFve2GldHn2ZZRqd25ib3[gS4FtPC2odYPl[EBpfW2jbjDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDh[pRmeiBzNjD0c{AzOCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxKD1iMD6wOFMh\|ryPLh?=	M4DXfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUK4OlAxLz5{MUGyPFYxODxxYU6=
HepG2	MY\GeY5kfGmxbjDhd5NigQ>?	NGnDT2cyOCC3bX;sM2w>	NVfCPYN[OjRiaILz	NHnmZ5ZKdmO{ZXHz[UBqdiCpbIXjc5NmKGOxboP1cZB1cW:wIHnuJIh2dWGwIFjldGczKGOnbHzzJIF1KDFyIIXtc4wwVCCjZoTldkAzPCCqcoOgdoVt[XSrdnWgeI8h[2:wdILvcC=>	NWPsWXo{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyOVY4PDhpPkKxNlU3PzR6PD;hQi=>
HepG2	MWfGeY5kfGmxbjDhd5NigQ>?			NUTuVW5yXHKjboPhZ5RqfmG2aX;uJI9nKGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiY3:teJJidnOoZXP0[YQhf2m2aDDQVHJGOy2WSz3MeYMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4xPSEQvF2u	MkXkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2NUC0OlgoRjJzNEWwOFY5RC:jPh?=
HepG2	NV3qSZpDTnWwY4Tpc44h[XO\|YYm=			Mm\4RYdwdmm\|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIHj1cYFvKEincFeyJINmdGy\|IHL5JHBRWkVvbIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4xOSEQvF2u	M3\3VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEiyOFQ3Lz5{MUS4NlQ1PjxxYU6=
CV1	M2rN[2Z2dmO2aX;uJIF{e2G7		MYq0NEBpenN?	NFrGN\|NC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZU1NSkRiZYjwdoV{e2WmIHnuJGNXOSClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKEejbESgZYZ1\XJiNECgbJJ{KGK7IHz1Z4ln\XKjc3WgZoF{\WRidILhcpNi[3SrdnH0bY9vKGG\|c3H5MEBGSzVyIE2gNE4yKM7:TT6=	M1m2UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUG1NFY{Lz5{MUWxOVA3OzxxYU6=
3T3L1	NWXhN4lNTnWwY4Tpc44h[XO\|YYm=	M1fBS\|ExKHWP		NXO1W49iUW6lcnXhd4UhcW5iYXTpdI9v\WO2aX6gcXJPSSCuZY\lcJMhcW5iVF7GZYxxcGFvaX7keYNm\CCvb4Xz[UA{XDOOMTDj[YxteyCycnX0doVifGWmIHH0JFExKHWPIHLl[o9z\SCWTl[tZYxxcGFiY3jhcIxmdmenIILlcIF1cX[nIITvJINwdnS{b3y=	NGS0flA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi1NVA5QSd-MkG4OVExQDl:L3G+
COS7	MkfpSpVv[3Srb36gZZN{[Xl?			MYXNc4R2dGG2aX;uJI9nKGi3bXHuJHBRSVKpYX3tZU1NSkRiZYjwdoV{e2WmIHnuJIFnemmlYX6g[5Jm\W5ibX;ub4V6KEORU{egZ4VtdHNiY3:teJJidnOoZXP0[YQhf2m2aDDHZYw1KGG\|c3Xzd4VlKGG\|IHHjeIl3[XSrb36gc4YhfHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JIJ6KGy3Y3nm[ZJie2ViYYPzZZktKEWFNUCgQUAxNjB2MzFOwG0v	NXvaNYJlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4O\|MxPzBpPkKxPFc{ODdyPD;hQi=>
Sf21	NGDaOnFHfW6ldHnvckBie3OjeR?=			Ml:wTY5pcWKrdHnvckBw\iCqdX3hckBDW0WSIHX4dJJme3OnZDDpckBxdGG\|bXGgcYVu[nKjbnWgeoV{cWOuZYOgc4YhW2Z{MTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEGWUD3k[ZBmdmSnboSgX\|NJZXSjdYLvZ4hwdGG2ZTD1dJRic2VuIFnDOVAhRSB4LkSg{txONg>?	M4qyelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOU[1OlI{Lz5{MUm2OVYzOzxxYU6=
Sf21	M2G1OmZ2dmO2aX;uJIF{e2G7			NUTtPIRpUW6qaXLpeIlwdiCxZjDTdJJi\3WnLVThe4xmgSC{YYSgRpNmeCCneIDy[ZN{\WRiaX6gdIxie22jIH3lcYJz[W6nII\ld4lkdGW\|IH;mJHNnOjFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBCXFBvZHXw[Y5l\W62IGuzTH11[XW{b3Poc4xifGVidYD0ZYtmNCCLQ{WwJF0hPDBizszNMi=>	NFLUfnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm2OVYzOyd-MkG5OlU3OjN:L3G+
COS-1	MnTvSpVv[3Srb36gZZN{[Xl?		NYOwRmZiOjRiaILz	NEPMPVFC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJGNQWy1zIHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWtZoF{\WRibIXtbY5wdWW2cnnjJIFv[Wy7c3nzMEBGSzVyIE2gNE4xPDhizszNMi=>	MoGyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyNUGwOVQoRjJ{MEWxNFU1RC:jPh?=
COS1	MWTGeY5kfGmxbjDhd5NigQ>?			MX;CbY5lcW6pIHHm[olvcXS7IITvJIh2dWGwIIfpcIQhfHmyZTDQVGFT\2GvbXGgUGJFKGW6cILld5Nm\CCrbjDDU3MyKGOnbHzzJINwNWW6cILld5NqdmdiR1HMOEBjgSC\|Y3nueIltdGG2aX;uJJBzd3irbXn0fUBie3OjeR?=	M1XTTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEewOlA1Lz5{MkC3NFYxPDxxYU6=
HepG2	NHjwO4xHfW6ldHnvckBie3OjeR?=		MVWyNEBpenN?	M4H6UmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gS2FNPC2odYPl[EBRWEGUZ3HtcYEhdGmpYX7kJIJqdmSrbneg[I9u[WmwIHX4dJJme3OnZDDpckBJ\XCJMjDj[YxteyCjZoTldkAzOCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJJRz[W6\|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEKg{txONg>?	NIrXc4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC4NVk{Oid-MkKwPFE6OzJ:L3G+
BL21 DE3	MoTtSpVv[3Srb36gZZN{[Xl?			MnvBSIl{eGyjY3Xt[Y51KG:oIGuzTH1zd3OrZ3zpeIF7d26nIH\yc40hVi22ZYLtbY5idCCKaYOteIFo\2WmIHj1cYFvKFCSQWLnZY1u[SCuaXfhcoQh[mmwZHnu[{Bld22jaX6g[ZhxemW\|c3XkJIlvKEW\|Y3jldolkcGmjIHPvcIkhSkx{MTDESVMh[2WubIOgZpkhe2OrboTpcIxifGmxbjDwdo95cW2rdImgZZN{[XluIFvpJF0hOC5yN{Sg{txONg>?	NILyXVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC4NVk{Oid-MkKwPFE6OzJ:L3G+
COS1	NG\FWolHfW6ldHnvckBie3OjeR?=		MYeyOEBpenN?	M3vqbXRz[W6\|YXP0bZZifGmxbjDv[kBpfW2jbjDmeYxtKGynbnf0bEBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFPPV\|Eh[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBTYFKjbIDoZUBi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5zMjFOwG0v	NUDwe\|lGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxPVc{QTZpPkKyNVk4Ozl4PD;hQi=>
MG-63	NWrDUotpTnWwY4Tpc44h[XO\|YYm=			MV\B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYTDlfJBz\XO\|ZXSgbY4hVUdvNkOgZ4VtdHNiYomgdoVxd3K2ZYKg[4Vv\S2kYYPl[EB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkCxNUDPxE1w	NHH0[Gc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkKyOVY1OSd-MkKyNlU3PDF:L3G+
293H DA	NHHFNppHfW6ldHnvckBie3OjeR?=		NULtcYZpOTZiaILz	MWnB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBNSkRiaX6gbJVu[W5iMkmzTEBFSSClZXzsd{Bi\nSncjCxOkBpenNiYomgWHIuTlKHVDDhZ5RqfmG2aX;uJJJmeG:{dHXyJIF{e2G7LDDFR\|UxKD1iMD6wNFI1KM7:TT6=	NF7MSoc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW4Nlk4Oyd-MkK1PFI6PzN:L3G+
MG-63	MmrKSpVv[3Srb36gZZN{[Xl?		NXvNeopnOjRiaILz	M13hZm1w\HWuYYTpc44hd2ZiZoXscE1t\W6pdHigbJVu[W5icGPHOU1nfXOnZDDQVGFT\2GvbXGg[ZhxemW\|c3XkJIlvKE2JLU[zJINmdGy\|IHPvMYV5eHKnc4PpcocheEeYLWCyMXBRWkViYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBj[XOnZDD0doFve2GldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjBzMTFOwG0v	NGS0cIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkeyO\|Q1QCd-MkK3Nlc1PDh:L3G+
Ac2F	MWfGeY5kfGmxbjDhd5NigQ>?	NITVS5IxNjFidH:gNVAhfU1?	MUe2JIhzew>?	NHnZS5FC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCrbjDyZZQhSWN{RjDj[YxteyCjc4Pld5Nm\CCjczDseYNq\mW{YYPlJIFkfGm4aYT5JIF1KDBwMTD0c{AyOCC3TTDh[pRmeiB4IHjyd{BjgSC{ZYDvdpRmeiCpZX7lJIF{e2G7	NIrQdFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkixPVE6OCd-MkK4NVkyQTB:L3G+
HepG2	M4nOd2Z2dmO2aX;uJIF{e2G7			M4\ydXRz[W6\|YXP0bZZifGmxbjDv[kBISUx2LX\1d4VlKFCSQWLnZY1u[SCuaXfhcoQh[mmwZHnu[{Bld22jaX6geJJidnOoZXP0[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5zIN88UU4>	M1vYNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUO0OVM4Lz5{MkmzOFU{PzxxYU6=
THP1	MWnGeY5kfGmxbjDhd5NigQ>?	MonsNVAxKHWP	MUezJIhzew>?	Mli2WJJidnOjY4TpeoF1cW:wIH;mJHBRSVKpYX3tZUBqdiCqdX3hckBVUFBzIHPlcIx{KGG\|c3Xzd4VlKGG\|IHTlZ5Jm[XOnIHnuJGxRWy2rbnT1Z4VlKE2FUEGgcXJPSSCneIDy[ZN{cW:wIHH0JFExOCC3TTDwdoVqdmO3YnH0[YQh\m:{IEOgbJJ{KHC{aX;yJJRwKEySUzDjbIFtdGWwZ3WgcYVie3W{ZXSgZYZ1\XJiMUigbJJ{KGK7IGLUMXBEWiCjbnHsfZNqeyC{ZXzheIl3\SC2bzD1cpRz\WG2ZXSgZ49vfHKxbB?=	NEjleng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmzOFU{Pyd-MkK5N\|Q2Ozd:L3G+
THP1	NYf1[IN5TnWwY4Tpc44h[XO\|YYm=	NUXjeVdWOTByIIXN	MYWzJIhzew>?	MVPUdoFve2GldHn2ZZRqd25ib3[gVHBCWmejbX3hJIlvKGi3bXHuJHRJWDFiY3XscJMh[XO\|ZYPz[YQh[XNiZHXjdoVie2ViaX6gUHBUNWmwZIXj[YQhUUx4IH3SUmEh\XiycnXzd4lwdiCjdDCxNFAhfU1icILlbY5kfWKjdHXkJIZweiB\|IHjyd{BxemmxcjD0c{BNWFNiY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDF6IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZMhemWuYYTpeoUhfG9idX70doVifGWmIHPvcpRzd2x?	NFXCNJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmzOFU{Pyd-MkK5N\|Q2Ozd:L3G+
HepG2	NUPQU4pHTnWwY4Tpc44h[XO\|YYm=	Mn\UNVAhfU1?	NUjWeXlbOjRiaILz	NUe4cpM6WmWmdXP0bY9vKG:oIHfseYNwe2ViY3;ud5VueHSrb36gbY4hcW6\|dXzpck1z\XOrc4ThcpQhcHWvYX6gTIVxTzJiY3XscJMh[XRiMUCgeW0h[W[2ZYKgNlQhcHK\|IHL5JIdtfWOxc3Wgc5hq\GG\|ZTDt[ZRpd2RiaX6gdJJme2WwY3Wgc4YhOC5zIIXNJI9nKGmwc4XsbY4>	NWi0NINlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNlUzPDRpPkKzNFI2OjR2PD;hQi=>
HepG2	M3HsZmZ2dmO2aX;uJIF{e2G7	M3rNN\|ExKHWP	M1fjRlI1KGi{cx?=	NX:zRlY4WmWmdXP0bY9vKG:oIHfseYNwe2ViY3;ud5VueHSrb36gbY4hcW6\|dXzpck1z\XOrc4ThcpQhcHWvYX6gTIVxTzJiY3XscJMh[XRiMUCgeW0h[W[2ZYKgNlQhcHK\|IHL5JIdtfWOxc3Wgc5hq\GG\|ZTDt[ZRpd2RiaX6gdJJme2WwY3Wgc4YhOjJwMjDtUUBw\iCpbIXjc5Nm	M{XOU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MEK1NlQ1Lz5{M{CyOVI1PDxxYU6=
HepG2	MmTWSpVv[3Srb36gZZN{[Xl?		MkfaNlAhcHK\|	MnPwWJJidnOjY4TpeoF1cW:wIH;mJGdCVDRvZoXz[YQhcHWvYX6gVHBCWmejbX3hJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gTIVxTzJiY3XscJMh[W[2ZYKgNlAhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMEO5JO69VS5?	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF5MUC0OUc,OjNzN{GwOFU9N2F-
L02	NUmyZoJ3TnWwY4Tpc44h[XO\|YYm=		M3rvPVI1KGi{cx?=	NYjhdoxVSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFvTFLEJIV5eHKnc4Pl[EBqdiCqdX3hckBNODJiY3XscJMh[29vZYjwdoV{e2mwZzDwS2w{NVOYNECtS2FNPCCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIJie2WmIITyZY5{[WO2aY\heIlwdiCjc4PhfUwhTUN3MDC9JFAvQSEQvF2u	M{KxTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MUi2N\|A4Lz5{M{G4OlMxPzxxYU6=
HEK293	MWTGeY5kfGmxbjDhd5NigQ>?		NWHYNJZxPDhiaILz	NWrSO\|NjSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{Bkd3S{YX7z[oVkfGWmIIfpeIghWFCURYi0MXRMNWy3YzDhd5Nme3OnZDDhd{Bi[3SrdnH0bY9vKG:oIHz1Z4ln\XKjc3WgZYN1cX[rdImgcYVie3W{ZXSgZYZ1\XJiNEigbJJ{KGK7IITyZY5{[WO2aY\heIlwdiCjc4PhfUwhTUN3MDC9JFQ{NjdzIN88UU4>	MlTEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NkW4OFQoRjJ\|Mk[1PFQ1RC:jPh?=
HEK293	MWTGeY5kfGmxbjDhd5NigQ>?	NVHYN2E{OTBidV2=		M3O4enRz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJINwNXS{YX7z[oVkfGWmIIfpeIghSVB{LWDQVmUh[XRiMUCgeW0h[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5	NFyzZWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K3N\|UyQSd-MkOyO\|M2OTl:L3G+
HEK293	MYrGeY5kfGmxbjDhd5NigQ>?			M{PQeWFod26rc4SgZZQhcHWvYX6gVHBCWmejbX3hJGxDTCCneIDy[ZN{\WRiaX6gbJVu[W5iSFXLNlk{KGOnbHzzJINwfHKjboPm[YN1\WRid3n0bEBISUx2LVz1Z{Bie3Onc4Pl[EBieyC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjByNDFOwG0v	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ6Nke4O{c,OjN{OE[3PFc9N2F-
HEK293	MV7GeY5kfGmxbjDhd5NigQ>?		NVGxWm9yOjRiaILz	NFvkdXVVemGwc3HjeIl3[XSrb36gc4YhT0GONDDERmQu\nW\|ZXSgbJVu[W5iUGDBVodidW2jLVzCSEBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjFizszNMi=>	NY\aS2ViRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyPVQ5OzBpPkKzNlk1QDNyPD;hQi=>
CMT93	M1vNb2Z2dmO2aX;uJIF{e2G7	MoPiNVAhfU1?	MVezNEBucW6\|	Mk\5TY5lfWO2aX;uJI9nKFCSQWKt[4FudWFicILvcY91\XJiYXP0bZZqfHliaX6gUHBUNXO2aX31cIF1\WRibX;1d4UhS02WOUOgZ4VtdHNiYYSgNVAhfU1icILlbY5kfWKjdHXkJIZweiB\|MDDtbY5{KGKnZn;y[UBNWFNvY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDZiaILzJJBwe3RiY3jhcIxmdmenIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigQ>?	M{H6T\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEG2NVkxLz5{M{SxOlE6ODxxYU6=
HepG2	M4i0O2Z2dmO2aX;uJIF{e2G7			MX\B[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRvZoXz[YQhWFCDUnfhcY1iKE12NkPBJI12fGGwdDCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBpfW2jbjDI[ZBIOiClZXzsd{BjgSC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlAxOiEQvF2u	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzVyMkKxNkc,OjN3MEKyNVI9N2F-
HepG2	NGXtZldHfW6ldHnvckBie3OjeR?=			NEWxfJFC\2:waYP0JIFkfGm4aYT5JIF1KEeDTESt[pV{\WRiUGDBVodidW2jIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJIh2dWGwIFjldGczKGOnbHzzJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNFEh\|ryPLh?=	MojUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MEKyNVIoRjJ\|NUCyNlEzRC:jPh?=
L6	MnntSpVv[3Srb36gZZN{[Xl?		Ml[5NlQhcHK\|	MojnTY5lfWO2aX;uJI9nKGeudXPvd4UhfXC2YXvlJIlvKHKjdDDMOkBk\WyuczDweYx{\WRid3n0bEBEOTRvZHXvfJkh\2y3Y3;z[UBi\nSncjCyOEBpenNiaX6gdJJme2WwY3Wgc4YhcW6\|dXzpckwhTUN3MDC9JFQvPDlizszNMi=>	NG\zWG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m5Nlg3Oid-MkO5PVI5PjJ:L3G+
L6	MV3GeY5kfGmxbjDhd5NigQ>?		NUTESXhuOTZiaILz	M4DlUGlv[3KnYYPlJIlvKDJvW{PIYU1l\W:6eXfseYNwe2VidYD0ZYtmKGmwIILheEBNPiClZXzsd{Bi\nSncjCxOkBpenNiYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yCjbnHsfZNqeyxiSVO1NEA:KDRwODFOwG0v	NGHRXnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEixN\|c{QCd-MkS4NVM4Ozh:L3G+
HEK293	M4jobmZ2dmO2aX;uJIF{e2G7	MXKxNEB2VQ>?	M2rLNFI1KGi{cx?=	M2nUe3Rz[W6\|YXP0bZZifGmxbjDv[kBRWEGULXfhcY1iKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{BifCBzMDD1UUBi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6	MkfsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6OUCwPVAoRjJ2OEmwNFkxRC:jPh?=
HEK293	NFq4eI5HfW6ldHnvckBie3OjeR?=		NVfFeVlbOjRiaILz	MYLB[49vcXO2IHHjeIl3cXS7IHH0JI1wfXOnIGDQRXJo[W2vYTDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHPvMYV5eHKnc4Ppcochf2m2aDDHZYw1KHKncH;yeIVzKH[nY4TvdkBi\nSncjCyOEBpenNiYomg[JVidC2udXPp[oVz[XOnIILldI9zfGW{IHHzd4F6NCCHQ{WwJF0hOC5yMzFOwG0v	MnfLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NUW4PFkoRjJ2OUW1PFg6RC:jPh?=
DU145	M2jnZ2N6fG:2b4jpZ4l1gSCjc4PhfS=>		M2PUSFQ5KGi{cx?=	M2HtTmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGRWOTR3IHPlcIx{KGG\|c3Xzd4VlKGG\|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzNjFOwG0v	M1vifFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUm2NVQ{Lz5{NEm5OlE1OzxxYU6=
PC3	NWexTIZMS3m2b4TvfIlkcXS7IHHzd4F6		M4LtXlQ5KGi{cx?=	NXvw[YFlS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWEN\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB{MD6zJO69VS5?	MlK5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7OU[xOFMoRjJ2OUm2NVQ{RC:jPh?=
MDA-MB-231	MnPDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NXvaUmp1OjRiaILz	MVrHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNSGEuVUJvMkOxJINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB3LkKzJO69VS5?	M4GzblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mke4NlM3Lz5{NUK3PFI{PjxxYU6=
HEK293	MknhSpVv[3Srb36gZZN{[Xl?			NXXudHUxSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlAyKM7:TT6=	M3v5fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C1Olg5Lz5{NUOwOVY5QDxxYU6=
HepG2	Ml7ISpVv[3Srb36gZZN{[Xl?		NHHrPZQzOCCqcoO=	M3nJWnRz[W6\|YXP0bZZifGmxbjDv[kBRWEGUIITyZY5{\mWldHXkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFIxKGi{czDifUBRWEGULXz1Z4ln\XKjc3WgZZN{[XluIFXDOVAhRSBzLk[g{txONg>?	MnW0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{ezNFQoRjJ3NEO3N\|A1RC:jPh?=
HEK293	M2LjdGZ2dmO2aX;uJIF{e2G7		MX[yOEBpenN?	NID0OodVemGwc3HjeIl3[XSrb36gc4YhWFCDUnfhcY1iKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{BqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk>	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR2MkOyNkc,OjV2NEKzNlI9N2F-
HepG2	Mmm5SpVv[3Srb36gZZN{[Xl?			MoruRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDHRWw1NVCSQWLnZY1u[SCuaXfhcoQh[mmwZHnu[{Bld22jaX6g[ZhxemW\|c3XkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODN7IN88UU4>	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MkK4NUc,OjV2NkKyPFE9N2F-
HepG2	NGj1PJJHfW6ldHnvckBie3OjeR?=		MkniNlAhcHK\|	NGL4eHpC\2:waYP0JIFkfGm4aYT5JIF1KEeDTEStSG5CKGKrbnTpcoch\G:vYXnuJIZ2e2WmIHj1cYFvKFCSQWLnZY1u[SCuaXfhcoQh[mmwZHnu[{Bld22jaX6g[ZhxemW\|c3XkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGG\|c3Xzd4VlKGG\|IILlZ4VxfG:{IITyZY5{[WO2aY\heIlwdiCrbnP1ZoF1\WRiZn;yJFIxKGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkC0JO69VS5?	Mo\nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUexN\|IoRjJ3NEm3NVMzRC:jPh?=
HepaR	M4roeGZ2dmO2aX;uJIF{e2G7	NVTnbop1OSC3TR?=	NGC2b5EyKGSjeR?=	NWj4WpZbSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gbJVu[W5iSHXwZXIh[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iRlHCVFEh\2WwZTDlfJBz\XO\|aX;uJIF1KDFidV2gbY5kfWKjdHXkJIZweiBzIHThfUBjgSCzdXHueIl1[XSrdnWgVGNTKG2ndHjv[EBz\WyjdHn2[UB1dyC3boTy[YF1\WRiY3;ueJJwdA>?	MnzqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUexN\|IoRjJ3NEm3NVMzRC:jPh?=
HepaR	M3z2ZWZ2dmO2aX;uJIF{e2G7	MUSxJJVO	NIPYT48yKGSjeR?=	NGTsPXZC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCrbjDoeY1idiCKZYDhVkBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCDTlfQWGw1KGenbnWg[ZhxemW\|c3nvckBifCBzIIXNJIlv[3WkYYTl[EBnd3JiMTDkZZkh[nlicYXhcpRqfGG2aY\lJHBEWiCvZYToc4QhemWuYYTpeoUhfG9idX70doVifGWmIHPvcpRzd2x?	NFG4XI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS5O\|E{Oid-MkW0PVcyOzJ:L3G+
HepaR	NGThSIVHfW6ldHnvckBie3OjeR?=	MlvkNUB2VQ>?	NYjoRm8xOSCmYYm=	MUDB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCqdX3hckBJ\XCjUjDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBJVUeFU{Kg[4Vv\SCneIDy[ZN{cW:wIHH0JFEhfU1iaX7jeYJifGWmIH\vdkAyKGSjeTDifUByfWGwdHn0ZZRqfmViUFPSJI1mfGixZDDy[YxifGm4ZTD0c{B2dnS{ZXH0[YQh[2:wdILvcC=>	Mo\kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUexN\|IoRjJ3NEm3NVMzRC:jPh?=
C2C12	Ml\OSpVv[3Srb36gZZN{[Xl?	MmnRN\|AhfU1?	M4fF[\|IhcHK\|	MoT5TY5lfWO2aX;uJI9nKDJvZHXvfJkuYzOKXT3ncJVkd3OnIIXweIFs\SCrbjDtc5V{\SCFMlOxNkBk\WyuczDheEA{OCC3TTDh[pRmeiB{IHjyd{BjgSCuaYH1bYQhe2OrboTpcIxifGmxbjDjc5VvfGmwZzDhcoFtgXOrcx?=	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh\|NUWzO{c,OjV6M{W1N\|c9N2F-
MCF7	M4PIbmZ2dmO2aX;uJIF{e2G7		NULTN3ZOOTZiaILz	M3i3NWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJJRz[W6\|ZnXjeIVlKGmwIHj1cYFvKE2FRkegZ4VtdHNiYX\0[ZIhOTZiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFg4KM7:TT6=	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ{NkS5NEc,OjZ{Mk[0PVA9N2F-
HEK293	MlXjSpVv[3Srb36gZZN{[Xl?	M3fCbVExKHWP	MXKyOEBpenN?	MXLB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZZN{\XO\|ZXSgZZMhemWlZYD0c5IhfHKjboPhZ5RqfmG2aX;uJIF1KDFyIIXNJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	NUDQZ3pGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zPFQzQDZpPkK2N\|g1Ojh4PD;hQi=>
3T3L1	MUjGeY5kfGmxbjDhd5NigQ>?	NIDqfYQzKHWP		M2C0THRz[W6\|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5ibX;1d4UhO1R\|TEGgZ4VtdHNiYYPz[ZN{\WRiYYOgZYRqeG:leYTlJIRq\m[ncnXueIlifGmxbjDifUBu\WG\|dYLpcochdGmyaXSgZYNkfW23bHH0bY9vKGG2IEKgeW0h[nliT3nsJHJm\CCRIIP0ZYlvcW6pLXLhd4VlKGG\|c3H5	Mlm0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUW3OFkoRjJ4NUm1O\|Q6RC:jPh?=
3T3L1	MnL4SpVv[3Srb36gZZN{[Xl?	MWGyJJVO		NInLVJVVemGwc3HjeIl3[XSrb36gc4YhWFCDUnfhcY1iKGmwIH3veZNmKDOWM1yxJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKE[DQmC0JIV5eHKnc4Ppc44h[XRiMjD1UUBjgSCzUFPSJI1mfGixZB?=	NYLD[HhGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVU4PDlpPkK2OVk2PzR7PD;hQi=>
3T3L1	Mne3SpVv[3Srb36gZZN{[Xl?	NY\W[21QOiC3TR?=		NHfDbpdVemGwc3HjeIl3[XSrb36gc4YhWFCDUnfhcY1iKGmwIH3veZNmKDOWM1yxJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEeWVWS0JIV5eHKnc4Ppc44h[XRiMjD1UUBjgSCzUFPSJI1mfGixZB?=	MkLKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUW3OFkoRjJ4NUm1O\|Q6RC:jPh?=
3T3L1	NFXaVZJHfW6ldHnvckBie3OjeR?=	NYLB[4FEOiC3TR?=		MlrlWJJidnOjY4TpeoF1cW:wIH;mJHBRSVKpYX3tZUBqdiCvb4Xz[UA{XDOOMTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBi\Gmyb37lZ5RqdiCneIDy[ZN{cW:wIHH0JFIhfU1iYomgdXBEWiCvZYToc4Q>	NWHyU49IRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVU4PDlpPkK2OVk2PzR7PD;hQi=>
3T3L1	NXr5c2tSTnWwY4Tpc44h[XO\|YYm=	NEj6b3IzKHWP		NG\QOpBVemGwc3HjeIl3[XSrb36gc4YhWFCDUnfhcY1iKGmwIH3veZNmKDOWM1yxJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEySTDDlfJBz\XO\|aX;uJIF1KDJidV2gZpkheVCFUjDt[ZRpd2R?	M2TKZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUm1O\|Q6Lz5{NkW5OVc1QTxxYU6=
HEK293	MkHjSpVv[3Srb36gZZN{[Xl?	MYexJJVO	NXfIdpNrOjRiaILz	M2LMeWFod26rc4SgZYN1cX[rdImgZZQhWFCDUjDnZY1u[SBqdX7rco94diCxcnnnbY4qKHS{YX7z[oVkfGWmIHnuJGhGUzJ7MzDj[YxteyCjdDCxJJVOKGGodHXyJFI1KGi{czDifUBlfWGuLXz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjl6OEOwOEc,OjZ7OEizNFQ9N2F-
C2C12	M1WwUmZ2dmO2aX;uJIF{e2G7	M1v3WlIxKHWP	MoO0OEBpenN?	MkLWWJJidnOjY4TpeoF1cW:wIH;mJHBRSVKpYX3tZUBqdiCvb4Xz[UBEOkNzMjDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBIVFWWLUSgcYVlcWG2ZXSgNk1PSkSJIIXweIFs\SCjdDCyNEB2VSCjdDC0JIhzeyCkeTDmcJVwemW\|Y3XuZ4Uh[XO\|YYm=	MoPxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMEC5PVMoRjJ5MUCwPVk{RC:jPh?=
HEK293	MmT3SpVv[3Srb36gZZN{[Xl?		M2fVSlI1KGi{cx?=	M{Lp[WFod26rc4SgZYN1cX[rdImgZZQhT0GOND30ZYdo\WRiaIXtZY4hWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDjbIlu\XKrYzDy[YNmeHSxcjDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlI3KM7:TT6=	Ml3FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3OUGwNFYoRjJ5NUmxNFA3RC:jPh?=
HepG2	NHn6[3FHfW6ldHnvckBie3OjeR?=		M2nXcFIxKGi{cx?=	MUjUdoFve2GldHn2ZZRqd25ib3[gS2FNPC2odYPl[EBpfW2jbjDQVGFT\2GvbXGgUGJFKGW6cILld5Nm\CCrbjDoeY1idiCKZYDHNkBk\WyuczDh[pRmeiB{MDDodpMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyIE2gNE4xPCEQvF2u	Mlf2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyN{[4NlcoRjJ6MEe2PFI4RC:jPh?=
HT-29	NHfmcoZHfW6ldHnvckBie3OjeR?=	MmnaNVAhfU1?	MX:yOEBpenN?	NIHy[YhC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SCrbjDoeY1idiCKVD2yPUBk\WyuczDoZZJjd3KrbnegRXBEKG23dHHueEBie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHdvfC:kZYThMYNifGWwaX6gd4lodmGuaX7nJJBifGi5YYmgZpkhdWWjc4XybY5oKGSnY4LlZZNmKGmwIFP5Z4xqdkRzIHzleoVtKGG2IEGwJJVOKHS{ZXH0[YQh\m:{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5QhdWW2aH;k	NFu1THY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC3OlgzPyd-MkiwO\|Y5Ojd:L3G+
COS7	NGjxbVdHfW6ldHnvckBie3OjeR?=		M2nTOVI1KGi{cx?=	NE[0OY9VemGwc3HjeIl3[XSrb36gc4YhcHWvYX6gS4FtPC2odYPl[EBRWEGUZ3HtcYEhVEKGIHX4dJJme3OnZDDpckBC\nKrY3HuJIdz\WWwIH3vcotmgSCFT2O3JINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlAxPCEQvF2u	NIHtTYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[wPVcxQCd-Mki2NFk4ODh:L3G+
COS7	MW\GeY5kfGmxbjDhd5NigQ>?		NXflflZGOjRiaILz	NIO3SnpVemGwc3HjeIl3[XSrb36gc4YhcHWvYX6gS4FtPC2odYPl[EBRWEGUYXzwbIEhVEKGIHX4dJJme3OnZDDpckBC\nKrY3HuJIdz\WWwIH3vcotmgSCFT2O3JINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCxNEDPxE1w	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZyOUewPEc,Ojh4MEm3NFg9N2F-
HEK293	MljlSpVv[3Srb36gZZN{[Xl?		M1;BXVE5KGi{cx?=	M4HsV2Fod26rc4SgZYN1cX[rdImgZZQhT0GOND3meZNm\CCSUFHS[4FudWFiTFLEJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WOncITvdkB1emGwc3HjeIl3[XSrb36gZYZ1\XJiMUigbJJ{KGK7IHT1ZYwhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxKD1iMD6wOEDPxE1w	NFGzTXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[2O\|g4Pid-Mki2Olc5PzZ:L3G+
Ac2F	MYHGeY5kfGmxbjDhd5NigQ>?	NUSxTIJ{PSC3TR?=	M32zPFYhcHK\|	NYLrb4NZSWexbnnzeEBi[3Srdnn0fUBifCCodXzsJIxmdme2aDDoeY1idiCobHHnMZRi\2enZDDQVGFT\2GvbXGxJGxDTCCneIDy[ZN{\WRiaX6gdoF1KEGlMl[gZ4VtdHNiY3;lfJBz\XO\|aX7nJHBRWkVvWEOtWGsh[XRiNTD1UUBi\nSncjC2JIhzeyCkeTDvcoUu\2yxIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	M4\BSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{i5NVIyLz5{OUO4PVEzOTxxYU6=
SK-N-MC	NF74ZYtyUFSVIHHzd4F6			MlHDdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO=	NGXyVG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
3T3-L1	M{O2S2Z2dmO2aX;uJIF{e2G7	NV2zfnM5OSC3TR?=	MVWxOUBl[Xm\|	MU\B[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCvb4Xz[UA{XDNvTEGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hS0R\|NjDtVm5CKGW6cILld5Nqd25iYYSgNUB2VSCjZoTldkAyPSCmYYnzJIJ6KHG3YX70bZRifGm4ZTDy[YFtNXSrbXWgVGNTKG2ndHjv[C=>	M{GwS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OEe4O\|Y4Lz5{OUi3PFc3PzxxYU6=
3T3-L1	NI[3O4dHfW6ldHnvckBie3OjeR?=	MV6xJJVO	M1n2dVE2KGSjeYO=	NU[wU3pjSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gcY92e2ViM2SzMWwyKGOnbHzzJIF{e2W\|c3XkJIF{KGmwZIXjeIlwdiCxZjDh[Ilxd2O7dHWg[Iln\mW{ZX70bYF1cW:wIHH0JFEhfU1iYX\0[ZIhOTViZHH5d{BjgSCRaXygVoVlKE9ic4ThbY5qdmdvYnHz[YQh[XO\|YYm=	Mom3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl6N{i3OlcoRjJ7OEe4O\|Y4RC:jPh?=
3T3-L1	Mmf4SpVv[3Srb36gZZN{[Xl?	MnLmNUB2VQ>?	M{fiOFE2KGSjeYO=	M1vvbWFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKGmwIH3veZNmKDOWMz3MNUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCFUGSxRUBuWk6DIHX4dJJme3Orb36gZZQhOSC3TTDh[pRmeiBzNTDkZZl{KGK7IIH1ZY51cXSjdHn2[UBz\WGuLYTpcYUhWEOUIH3leIhw\A>?	NGrD[og9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUi3PFc3Pyd-Mkm4O\|g4Pjd:L3G+
HepG2	NIDwV49HfW6ldHnvckBie3OjeR?=		NH3yOWkzOCCqcoO=	NWjTNZRUXHKjboPhZ5RqfmG2aX;uJI9nKEeDTESteIFo\2WmIHj1cYFvKFCSQWLnZY1u[SCOQlSg[ZhxemW\|c3XkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFIxKGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCzPUDPxE1w	Ml:3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzOUmyOVMoRjNyMUm5NlU{RC:jPh?=
3T3L1	MUjGeY5kfGmxbjDhd5NigQ>?	NInKeJY2KHWP		NGDPO\|NKdmS3Y4Tpc44hd2ZibHnwbYQh[WOldX31cIF1cW:wIHnuJI1wfXOnIEPUN2wyKGOnbHzzJIF1KDVidV2gZpkhV2muIGLl[EBQKHO2YXnubY5oNWKjc3XkJIF{e2G7	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODF7OUK1N{c,OzBzOUmyOVM9N2F-
3T3L1	NWTsV2N3TnWwY4Tpc44h[XO\|YYm=	Mn3jNkB2VQ>?		NGi4TmJKdmS3Y4Tpc44hd2ZibHnwbYQh[WOldX31cIF1cW:wIHnuJI1wfXOnIEPUN2wyKGOnbHzzJIF1KDJidV2gZpkhV2muIGLl[EBQKHO2YXnubY5oNWKjc3XkJIF{e2G7	MkXPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzOUmyOVMoRjNyMUm5NlU{RC:jPh?=
HepG2	NInUOlJHfW6ldHnvckBie3OjeR?=	MXi+NVAxKG6P	MlrnNlAhcHK\|	NYXXU4I6XHKjboPhZ5RqfmG2aX;uJI9nKEeDTESteIFo\2WmIHj1cYFvKFCSQWLnZY1u[SCOQlSg[ZhxemW\|c3XkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGG2IE6xNFAhdk1iYX\0[ZIhOjBiaILzJIlvKHC{ZYPlcoNmKG:oIEWgeW0hWFCDUnfhcY1iKGGwdHHnc45qe3RiR2e5OlYzKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	NVn0bIM{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CxPVkzPTNpPkOwNVk6OjV\|PD;hQi=>
COS7	MnHKSpVv[3Srb36gZZN{[Xl?		NI\tSYEzPCCqcoO=	NXjqcYtnSWexbnnzeEBi[3Srdnn0fUBifCC{ZXPvcYJqdmGwdDDoeY1idiCJQVy0MWRDTCCodYPl[EBRWEGUZ3HtcYEhVEKGIHX4dJJme3OnZDDpckBEV1N5IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkO5OkDPxE1w	Mn;JQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{MkC2NFIoRjNyMkKwOlAzRC:jPh?=
COS7	MWPGeY5kfGmxbjDhd5NigQ>?		MY[yOEBpenN?	NWjwOlNkSWexbnnzeEBi[3Srdnn0fUBifCC{ZXPvcYJqdmGwdDDoeY1idiCJQVy0MWRDTCCodYPl[EBRWEGUZ3HtcYEhVEKGIHX4dJJme3OnZDDpckBEV1N5IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkO5OkDPxE1w	M3f1WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkSxPVA4Lz5\|MEK0NVkxPzxxYU6=
HEK293	MWLGeY5kfGmxbjDhd5NigQ>?		NYPCW2NoOThiaILz	MlvHRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDHRWw1KG[3c3XkJHBRSVKpYX3tZU1NSkRiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjZoTldkAyQCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxKD1iMD6xNkDPxE1w	M3LxWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkm2Olg2Lz5\|MEK5OlY5PTxxYU6=
HEL 293H	MkPySpVv[3Srb36gZZN{[Xl?		NXfOdGFmOTZiaILz	NG\zSJlVemGwc3HjeIl3[XSrb36gc4YhT0GOND3ERmQh\nW\|ZXSgbJVu[W5iUGDBVodidW2jIHzp[4Fv\CCkaX7kbY5oKGSxbXHpckBmgHC{ZYPz[YQhcW5iVVHTMYJt[SCKRVygNlk{UCClZXzsd{BxemWrbnP1ZoF1\WRiZn;yJFE3KGi{czDmc4xtd3enZDDifUBHWkWWIIP1ZpN1emG2ZTDh[IRqfGmxbjDhcoQhdWWjc4Xy[YQh[W[2ZYKgNkBpenNiYomgWHIuTlKHVDDhd5NigSxiRVO1NEA:KDBwMEC4JO69VS5?	MkD3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB2MkmwPVcoRjNyNEK5NFk4RC:jPh?=
HEK293	M3;TbmZ2dmO2aX;uJIF{e2G7		Mlu4NVghcHK\|	MUnUdoFve2GldHn2ZZRqd25ib3[gbJVu[W5iUGDBVodidW2jIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiaX7jeYJifGWmIH\vdkAyQCCqcoOgZpkh\HWjbDDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjB\|OTFOwG0v	NETrUWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjFxJ{7DbGVOSkx:L3G+
CV-1	NUnnNIUzTnWwY4Tpc44h[XO\|YYm=			NWnR[VNXXGW\|dHXkJIZweiC2cnHud4FkfGm4YYTpc44hd2ZiaIXtZY4hWFCDUjDnZY1u[S2JYXy0JINpcW2ncnGgbY4hS1ZvMTDj[YxteyxiRVO1NEA:KDBwMEWg{txONg>?	MmDDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVIyNyd-Q3jFUWJNRC:jPh?=
3T3L1	M1O1N2Z2dmO2aX;uJIF{e2G7		NV;LWmFxPyCmYYnz	M2P3NXVxemWpdXzheIlwdiCxZjDGRXMheHKxdHXpckBmgHC{ZYPzbY9vKGmwIHTp[oZmemWwdHnheIVlKG2xdYPlJFNVO0xzIHPlcIx{KGGodHXyJFch\GG7czDifUB4\XO2ZYLuJIJtd3RiYX7hcJl{cXN?	NXzrSIdXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJzLze+R4hGVUKOPD;hQi=>
3T3L1	NUPhSWJrTnWwY4Tpc44h[XO\|YYm=		MmCwO{Bl[Xm\|	MkDGWZBz\We3bHH0bY9vKG:oIHHQNkBxem:2ZXnuJIV5eHKnc4Ppc44hcW5iZHnm[oVz\W62aXH0[YQhdW:3c3WgN3Q{VDFiY3XscJMh[W[2ZYKgO{Bl[Xm\|IHL5JJdme3Sncn6gZoxwfCCjbnHsfZNqew>?	M3vMUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=
3T3L1	MYHGeY5kfGmxbjDhd5NigQ>?		M4\1dVI1KGi{cx?=	MkHrTY5pcWKrdHnvckBw\iClaILvcolkKGmwc4XsbY4ue3SrbYXsZZRm\CCrboP1cIlvKHKnc3nzeIFv[2ViaX6g[Iln\mW{ZX70bYF1\WRibX;1d4UhO1R\|TEGgZ4VtdHNiYYPz[ZN{\WRiYYOgNk1l\W:6eT3EMXs{UF1vZ3z1Z49{\SC3cIThb4Uh[W[2ZYKgNlQhcHK\|IHL5JJNkcW62aXzsZZRqd25iY3;1cpRqdmdiYX7hcJl{cXN?	NWPxZXJwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJzLze+R4hGVUKOPD;hQi=>
3T3L1	MoPWSpVv[3Srb36gZZN{[Xl?	MUWxNEB2VQ>?	NGDLVWgzPCCqcoO=	NVPkbXg2UW6mdXP0bY9vKG:oIHnud5VtcW5vc4TpcZVt[XSnZDCyMYRmd3i7LVStX\|NJZS2pbIXjc5NmKHWydHHr[UBqdiCmaX\m[ZJmdnSrYYTl[EBud3W\|ZTCzWFNNOSClZXzsd{BifCBzMDD1UUBi\nSncjCyOEBpenNiYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yCjbnHsfZNqew>?	Mn;DQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVIyNyd-Q3jFUWJNRC:jPh?=
3T3L1	MkTGSpVv[3Srb36gZZN{[Xl?	MmDUNkB2VQ>?	NGXxXZUyPCCmYYnz	MXTB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVJiaX6gcY92e2ViM2SzUFEh[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iYXTpdI9kgXSnIHTp[oZmemWwdHnheIlwdiCjdDCyJJVOKGGodHXyJFE1KGSjeYOgZpkhV2muIGLl[EBQKHO2YXnubY5oKG2ndHjv[C=>	M3fuTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=
U2OS	M1nLU2Z2dmO2aX;uJIF{e2G7	NH:yTFQyOCC3TR?=	NXzoWnRoOjRiaILz	MUjBZ5RqfmG2aX;uJI9nKFCSQWLHJEh2dmuwb4fuJI9zcWerbjmg[ZhxemW\|c3XkJIlvKGi3bXHuJHUzV1NiY3XscJMh[XRiMUCgeW0hcW6ldXLheIVlKG[xcjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6	M{DhcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=

Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	Cyclin D1 / CDK2 / CDK4 / Bax / Bcl-2	29725405
Immunofluorescence	PPARγ / E-cadherin	27145370
Growth inhibition assay	Cell viability	29725405

In vivo

Rosiglitazone administration results in significant bone loss, including a decrease in bone volume, trabecular width, and trabecular number and an increase in trabecular spacing. Rosiglitazone also leads to a decrease in bone formation rate, with a simultaneous increase in fat content in the bone marrow. Rosiglitazone decreases the expression of the osteoblast-specific genes Runx2/Cbfa1, Dlx5, and alpha1(I)collagen, whereas the expression of the adipocyte-specific fatty acid binding protein aP2 is increased. ^[2] Rosiglitazone upregulates gene transcripts encoding mitochondrial proteins in white adipocytes from ob/ob mice accompanied by an increase in mitochondrial mass and changes in mitochondrial structure. ^[4]

Protocol (from reference)

References

+ Expand

Solubility (25°C)

In vitro	DMSO	71 mg/mL (198.64 mM)
	Water	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): 4% DMSO+30% PEG 300+5% Tween 80+ddH2O For best results, use promptly after mixing. Click to purchase: PEG300 Tween 80	5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	357.43
Formula	C₁₈H₁₉N₃O₃S
CAS No.	122320-73-4
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CN(CCOC1=CC=C(C=C1)CC2C(=O)NC(=O)S2)C3=CC=CC=N3

Download Rosiglitazone (BRL 49653) SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

Clinical Trial Information

NCT Number	Recruitment	Interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT02737709	Terminated	Drug: Paclitaxel and Carboplatin regimen	Non-small Cell Lung Cancer	Sun Yat-sen University	March 2016	Phase 2
NCT01402076	Completed	Drug: Tasimelteon\|Drug: Rosiglitazone\|Drug: Midazolam	Healthy Volunteers	Vanda Pharmaceuticals	August 2011	Phase 1
NCT01100619	Completed	Drug: rosiglitazone\|Drug: XL184	Papillary Thyroid Cancer\|Follicular Thyroid Cancer\|Huerthle Cell Thyroid Cancer\|Renal Cell Carcinoma	Exelixis	April 2010	Phase 1
NCT01415128	Completed	Drug: Omeprazole\|Drug: Rosiglitazone\|Drug: Desipramine	Erectile Dysfunction	VIVUS LLC	April 2010	Phase 1
NCT01376063	Completed	Drug: FG-4592	Healthy Adult Subjects	FibroGen	March 2010	Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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