Rosiglitazone (BRL 49653)

For research use only.

Catalog No.S2556

36 publications

Rosiglitazone (BRL 49653) Chemical Structure

CAS No. 122320-73-4

Rosiglitazone (BRL 49653) is a potent antihyperglycemic agent and a potent thiazolidinedione insulin sensitizer with IC50 of 12, 4 and 9 nM for rat, 3T3-L1 and human adipocytes, respectively. Rosiglitazone is a pure ligand of PPAR-gamma, and has no PPAR-alpha-binding action. Rosiglitazone modulates TRP channels and induces autophagy. Rosiglitazone prevents ferroptosis.

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Selleck's Rosiglitazone (BRL 49653) has been cited by 36 publications

Purity & Quality Control

Choose Selective PPAR Inhibitors

Biological Activity

Description Rosiglitazone (BRL 49653) is a potent antihyperglycemic agent and a potent thiazolidinedione insulin sensitizer with IC50 of 12, 4 and 9 nM for rat, 3T3-L1 and human adipocytes, respectively. Rosiglitazone is a pure ligand of PPAR-gamma, and has no PPAR-alpha-binding action. Rosiglitazone modulates TRP channels and induces autophagy. Rosiglitazone prevents ferroptosis.
Targets
PPAR [1]
(Cell-free assay)
TRPC [5]
()
Ferroptosis [6]
()
In vitro

Rosiglitazone markedly increase phosphorylation of threonine 172 within the α subunit of AMPK, with increased AMP:ATP ratio. Rosiglitazone has been reported to decrease cholesterol synthesis in a number of cell lines in a peroxisome proliferator-activated receptor γ-independent manner. Rosiglitazone activates both α1- and α2-containing AMPK complexes, and this leads to a marked increase in the phosphorylation of acetyl-CoA carboxylase. [1] Rosiglitazone activates PPAR-gamma2 which acts as a dominant inhibitor of osteoblastogenesis in murine bone marrow in vitro.[2] Rosiglitazone increases adiponectin secretion from omental cells up to 2.3-fold higher, whereas secretion from subcutaneous adipose cells is unaffected. [3] Rosiglitazone changes the morphological features and protein profiles of mitochondria in 3T3-L1 adipocytes. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 M4L0RWZ2dmO2aX;uJIF{e2G7 MXTF[oZm[3SrdnWgZ49v[2WwdILheIlwdiCjZ3HpcpN1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSHXwS|Ih[2WubIOsJGVEPTB;MD6wNkDPxE1? M4joVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OECxPFE4Lz5zNUiwNVgyPzxxYU6=
CV-1 M175OGZ2dmO2aX;uJIF{e2G7 NEHYNplKdiC4aYTyc{B1emGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBw\iCSZYLvfIl{d22nIIDyc4xq\mW{YYTvdkBi[3SrdnH0[YQhemWlZYD0c5Ih\2GvbXGgLHBRSVJrIHX4dJJme3OnZDDpckBEXi1zIHPlcIx{NCCHQ{WwJF0hOC5yNjFOwG0v NEfOO3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96NUe2PVA4Lz56NUe2PVA4RC:jPh?=
CV-1 NWTPd21xTnWwY4Tpc44h[XO|YYm= NE\ZbGNC[3SrdnH0bY9vKG:oIIDldo95cXOxbXWgdJJwdGmoZYLheI9zKGGldHn2ZZRm\CC{ZXPldJRweiCpYX3tZUBu\WG|dYLl[EBjgSCrbnT1Z5Rqd25ib3[gOVAmKG:oIH3hfIlufW1iYXzrZYxqdmVicHjvd5Bp[XSjc3WgZYN1cX[rdImsJJRz[W6|ZnXjeIlwdiCjc4PhfUBqdiCFVj2xJINmdGy|LDDFR|UxKD1iMD6wPFkyOyEQvF2u MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86QDN4NkKwK|46QDN4NkKwQE9iRg>?
3T3-L1 NYDC[IE1TnWwY4Tpc44h[XO|YYm= MnzTV5RqdXWuYYTpc44hd2ZiYXTpdI9o\W6nc3nzJIlvKDOWMz3MNUBk\WyuczDpd{BmgHC{ZYPz[YQh[XNiY3;uZ4VvfHKjdHnvckBmeXWrdnHs[Y51KHSxIITo[UBcKDFvMUTDYUB2eHSja3WgZ492dnS|IHHmeIVzKHS{ZXH0cYVvfCC5aYToJFAvOiC3Zz;tUEB1em:pbHn0ZZpwdmVuIFXDJF0hOC5yOTFOwG0v MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODl3NkKxN{c,OTB7NU[yNVM9N2F-
CV-1 NGXrSWpHfW6ldHnvckBie3OjeR?= NEfkR29O[XirbXHsJJJmeG:{dHXyJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUHXyc5hqe2:vZTDwdo9tcW[ncnH0c5Ih[WO2aY\heIVlKHKnY3XweI9zKGejbX3hJGdidDRiY3jpcYVzcWNiaX6geJJidnOrZX70cJkhfHKjboPm[YN1\WRiQ2[tNUBk\WyuczDifUBnfW6ldHnvcoFtKGG|c3H5MkwhTUN3MDC9JFAvODR|IN88UU4> NFnhO5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUeyNFg2PCd-MUG3NlA5PTR:L3G+
COS1 NY\2e4hHTnWwY4Tpc44h[XO|YYm= MmT6RYdwdmm|dHnjJIFkfGm4aYT5JJdieyCmZYTldo1qdmWmIHnuJGNQWzFiY3XscJMhfHKjboPm[YN1\WRid3n0bEBISUxiND3QVGFTKGejbX3hJJJm[2WydH;yMEBGSzVyIE2gNE4xOjFizszNMi=> MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjZzN{myOEc,OTJ4MUe5NlQ9N2F-
COS1 NYr3R3FrTnWwY4Tpc44h[XO|YYm= NIDKTVdKdiC4aYTyc{Bi\2:waYP0JIFkfGm4aYT5JJRme3SnZDDmc5IhfHKjboPhZ5RqfmG2aX;uJIlvKGi3bXHuJHBRSVJiZ3HtcYEuT2GuNDDjbIlu\XKrYzDDU3MuOSClZXzsd{whTUN3MDC9JFAvODJizszNMi=> M33wU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{N{K5OlY5Lz5zMkeyPVY3QDxxYU6=
COS1 NYXBdXgxTnWwY4Tpc44h[XO|YYm= NGXwUXNKdiC4aYTyc{B1emGwc3HjeIl3[XSrb36gc4YhcHWvYX6gdIVzd3irc3;t[UBxem:uaX\ldoF1d3JiYXP0bZZifGWmIILlZ4VxfG:{IHfhcY1iKG2nYYP1doVlKGmwIGDQRXIuT0GONDDjbIlu\XKrYzDDU3MuOSClZXzsd{whTUN3MDC9JFAvODJizszNMi=> M3\nSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OEezOVE4Lz5zMki3N|UyPzxxYU6=
CV-1 NV64TJFNTnWwY4Tpc44h[XO|YYm= M{HuOGlvKH[rdILvJIV3[Wy3YYTpc44h[WejaX7zeEBTYFJvYXzwbIEwWFCDUj3nZY1u[SCrbjDDWk0yKGOnbHzzJIJ6KGOxdILhcpNn\WO2aX;uJIF{e2G7IIfhd{Bl\XSncn3pcoVlNCCHQ{WwJF0hOC5|MkWg{txONg>? MlvCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ7NUSwOlEoRjF{OUW0NFYyRC:jPh?=
3T3-L1 MX\GeY5kfGmxbjDhd5NigQ>? MXXF[oZm[3SrdnWgZ49v[2WwdILheIlwdiCob4Kg[Y5p[W6lZX3lcpQhd2ZiaX7zeYxqdi2rbnT1Z4VlKHS{aXfsfYNmemmmZTDhZ4N2dXWuYYTpc44hcW5iM2SzMWwyKGOnbHzzMEBGSzNyIE2gNE4xOiEQvF2u MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDVyNU[0OEc,OTR3MEW2OFQ9N2F-
HepG2 NFWxRnRHfW6ldHnvckBie3OjeR?= NHPTVIpG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDh[4FqdnO2IHj1cYFvKFCSQWLnZY1u[SCneIDy[ZN{\WRiaX6gTIVxTzJiY3XscJMtKEWFNUCgQUAxNjB|OTFOwG0v M3ztNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4MUC3NVUxLz5zNkGwO|E2ODxxYU6=
U2OS NWDNUHdiTnWwY4Tpc44h[XO|YYm= MmfMSYZn\WO2IH;uJHBRSVKpYX3tZUB1emGwc3HjeIl3[XSrb36gZYN1cX[rdImgbY4hXTKRUzDj[YxteyxiRVO1NEA:KDBwMEOg{txONg>? NFLGZ3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkOwNFk1PCd-MU[zNFA6PDR:L3G+
Huh7 NY\q[5EzTnWwY4Tpc44h[XO|YYm= MU\GeY5kfGmxbnHsJIFkfGm4aYT5JIF1KGi3bXHuJHBRSVJiZ3HtcYEhcW5iSIXoO{Bk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkKyJO69VS5? NWDDNndFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[zOlY3ODFpPkG2N|Y3PjBzPD;hQi=>
Huh7 NX;EZ2s{TnWwY4Tpc44h[XO|YYm= MkfsSYZn\WO2IH;uJHBRSVJiZ3HtcYEhfHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JIlvKEi3aEegZ4VtdHNuIFXDOVAhRSByLkKyJO69VS5? NXnPO|ZjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0OVExQDdpPkG2OFUyODh5PD;hQi=>
HEK293 MmS0SpVv[3Srb36gZZN{[Xl? MVfB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDy[YNmeHSxcjDpcpRmemGldHnvckB4cXSqIIP0[ZJwcWRicnXj[ZB1d3JiY3;hZ5RqfmG2b4KtNUBjgSCHWV\QJIJie2WmIILldI9zfGW{IHflcoUh[XO|YYm= NVz3ZW4xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[2PFAyPTlpPkG2OlgxOTV7PD;hQi=>
HEK293 M3nmcGZ2dmO2aX;uJIF{e2G7 NV:1UVYzSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhemWlZYD0c5IhcW62ZYLhZ5Rqd25id3n0bEBz\XSrbn;p[EBZNXKnY3XweI9zKGGucHjhJIJ6KEW\RmCgZoF{\WRicnXwc5J1\XJiZ3Xu[UBie3OjeR?= NVLYeZUyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[2PFAyPTlpPkG2OlgxOTV7PD;hQi=>
CV1 MUPGeY5kfGmxbjDhd5NigQ>? M1Xxd3Rz[W6|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5iQ2[xJINmdGy|LDDFR|UxKD1iMD6wO|Yh|ryPLh?= NEW0cWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkiyNVc3QSd-MU[4NlE4Pjl:L3G+
NIH3T3 M2TOdGZ2dmO2aX;uJIF{e2G7 MWPBZ5Rqfmm2eTDheEBpfW2jbjDQVGFTKGejbX3hJJRz[W6|ZnXjeIVlKGmwIF7JTFNVOyClZXzsd{BjgSCudXPp[oVz[XOnIHHjeIl3cXS7IHHzd4F6NCCHQ{WwJF0hOC5|MjFOwG0v M{HQW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OEW0NFg2Lz5zNki1OFA5PTxxYU6=
CV1 NVHvU2FPTnWwY4Tpc44h[XO|YYm= MnHxWJJidnOjY4TpeoF1cW:wIH;mJIh2dWGwIGDQRXJo[W2vYTDpckBEXjFiY3XscJMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4{ODhizszNMi=> M17SWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUewN|kyLz5zNkm3NFM6OTxxYU6=
HepG2 MYfGeY5kfGmxbjDhd5NigQ>? M2P2V2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWiCpYX3tZUBqdiCjIFjldGczKGOnbHzzJIJ6KFCSQWKtS2FNPCC2cnHud4FkfGm4YYTpc44h[XO|YYmsJGVEPTBiPTCwMlE2QCEQvF2u MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl5M{O1PEc,OTZ7N{OzOVg9N2F-
HepG2 M4TBRmZ2dmO2aX;uJIF{e2G7 MV7B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXIh\2GvbXGgbY4hUGWyR{KgZ4VtdHNiYomgVHBCWi2JQVy0JJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMUW4JO69VS5? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl5OUO0NUc,OTZ7N{mzOFE9N2F-
CV1 M3fjZmZ2dmO2aX;uJIF{e2G7 MWfBZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGgbY4hS1ZzIHPlcIx{NCCHQ{WwJF0hOC5|MEig{txONg>? MlP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdyMEWzPVQoRjF5MEC1N|k1RC:jPh?=
Huh7 MnTGSpVv[3Srb36gZZN{[Xl? M2DlWGFkfGm4aYT5JIF1KGi3bXHuJHBRSVJiZ3HtcYEhcW5iSIXoO{Bk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkKyJO69VS5? NU\1WoNlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUewN|QyPDlpPkG3NFM1OTR7PD;hQi=>
CV1 MWXGeY5kfGmxbjDhd5NigQ>? MkPPRYdwdmm|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhcW5iQ2[xJINmdGy|IHL5JJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwM{C4JO69VS5? NUXCe5lpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexNlk4OjVpPkG3NVI6PzJ3PD;hQi=>
HepG2 MYrGeY5kfGmxbjDhd5NigQ>? NX3YN5U1SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUZ3HtcYEhcW5iSHXwS|Ih[2WubIOgZpkhWFCDUj3HRWw1KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuNFgzKM7:TT6= M{T1T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUW3NFE6Lz5zN{G1O|AyQTxxYU6=
HEK293 NHrUdGFHfW6ldHnvckBie3OjeR?= NG[3NWVC\2:waYP0JIFkfGm4aYT5JIF1KFCSQWLnZY1u[SC{ZXPldJRweiCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGK7IFfBUFQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6wN|Uh|ryPLh?= NWO0fHpoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUeyPVI3ODZpPkG3NlkzPjB4PD;hQi=>
HEK293 MoLqSpVv[3Srb36gZZN{[Xl? MnT3RYN1cX[rdImgZZQhcHWvYX6gZYRqeG:|ZTD0bZN{fWViUGDBVkBo[W2vYTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHL5JHBRSVJvR1HMOEB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkOxJO69VS5? NIjoWZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{O0N|M4OSd-MUezOFM{PzF:L3G+
L6 NX:4V2dOTnWwY4Tpc44h[XO|YYm= MX3F[oZm[3Rib36g[oF1fHliYXPp[EBwgGmmYYTpc44hcW5icnH0JGw3KGOnbHzzMEBGSzVyIE2gOUDPxE1w NYLnNmRyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezOFM{PzFpPkG3N|Q{OzdzPD;hQi=>
HepG2/C3A NGnOcI1HfW6ldHnvckBie3OjeR?= MkCyRYN1cX[jdHnvckBw\iCqdX3hckBRWEGUZ3HtcYEhdGmpYX7kJIJqdmSrbneg[I9u[WmwLX3l[IlifGWmIITyZY5{[3KrcITpc45idCCjY4Tpeol1gSCrbjDoeY1idiCKZYDHNk9EO0FiY3XscJMh[29vdILhcpNn\WO2ZXSge4l1cCCodYPl[EBI[Wx2LVzCSEBjgSClb4TyZY5{\mWldHnvckBie3OjeTygSWM2OCB;IECuNFA{QCEQvF2u NEH5UJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SwN|Y5QCd-MUe0NFM3QDh:L3G+
CV1 M3LmVWZ2dmO2aX;uJIF{e2G7 M1nKflI1KGi{cx?= M2rJXmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIlvKEOYMTDj[YxteyCkeTDHRWw1KHS{YX7zZYN1cX[jdHnvckBie3OjeTDh[pRmeiB{NDDodpMtKEWFNUCgQUAxNjB|IN88UU4> M1fDeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUC3NlI2Lz5zN{WwO|IzPTxxYU6=
CV1 NF3vWplHfW6ldHnvckBie3OjeR?= NUDSVphCOjRiaILz NX;wXHpGSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUYXzwbIEhcW5iQ2[xJINmdGy|IHL5JGdCVDRidILhcpNi[3SrdnH0bY9vKGG|c3H5JIFnfGW{IEK0JIhzeyxiRVO1NEA:KDNwNE[g{txONg>? M{j0UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUC3NlI2Lz5zN{WwO|IzPTxxYU6=
NIH3T3 NGP1ZopHfW6ldHnvckBie3OjeR?= NHzUNIhC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iTlnIN3Q{KGOnbHzzJIJ6KEeDTESgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5|MjFOwG0v M37hVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkK0O|c4Lz5zN{[yOFc4PzxxYU6=
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CV1 NWq1WWpOTnWwY4Tpc44h[XO|YYm= M4nyeGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmGucHjhJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS1ZzIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiZoXz[YQhT2GuND3ERmQh[nlidILhcpNi[3SrdnH0bY9vKGG|c3H5MEBGSzVyIE2gN{41PiEQvF2u MmrMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh6Mk[yNFUoRjF6OEK2NlA2RC:jPh?=
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CV1 NV:0eplMTnWwY4Tpc44h[XO|YYm= NYPKdmtFXHKjboPhZ5RqfmG2aX;uJI9nKEejbESt[pV{\WRiaIXtZY4hWFCDUnfhcY1iKESQQTDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS1ZzIHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvOSEQvF2u NYPXO2FPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxNVI4QDRpPkKxNVEzPzh2PD;hQi=>
HEK293 NVzBUVBiTnWwY4Tpc44h[XO|YYm= M{DsTVE3KHSxIEKwJIhzew>? M2PIN3Rz[W6|YXP0bZZifGmxbjDv[kBI[Wx2LX\1d4VlKGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIFnfGW{IEG2JJRwKDJyIHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTBiPTCwMlA1OyEQvF2u NXTOXJFTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxNlg3ODBpPkKxNVI5PjByPD;hQi=>
HepG2 Mkm2SpVv[3Srb36gZZN{[Xl? NGjibVAyOCC3bX;sM2w> MkX5NlQhcHK| NV3GbGZ4UW6lcnXhd4UhcW5iZ3z1Z49{\SClb37zeY1xfGmxbjDpckBpfW2jbjDI[ZBIOiClZXzsd{BifCBzMDD1cY9tN0xiYX\0[ZIhOjRiaILzJJJmdGG2aY\lJJRwKGOxboTyc4w> M4TXU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkW2O|Q5Lz5{MUK1Olc1QDxxYU6=
HepG2 NYX2NVBWTnWwY4Tpc44h[XO|YYm= NXXz[IppXHKjboPhZ5RqfmG2aX;uJI9nKGi3bXHuJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiY3:teJJidnOoZXP0[YQhf2m2aDDQVHJGOy2WSz3MeYMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4xPSEQvF2u NFvmRZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS1NFQ3QCd-MkG0OVA1Pjh:L3G+
HepG2 NH\5THpHfW6ldHnvckBie3OjeR?= M13mOGFod26rc4SgZYN1cX[rdImgZZQhWFCDUnfhcY1iKHKnY3XweI9zKGW6cILld5Nm\CCrbjDoeY1idiCKZYDHNkBk\WyuczDifUBRWFKHLXz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODFizszNMi=> NHzKVFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS4NlQ1Pid-MkG0PFI1PDZ:L3G+
CV1 MWHGeY5kfGmxbjDhd5NigQ>? NGLDdZc1OCCqcoO= MYDB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJo[W2vYT3MRmQh\XiycnXzd4VlKGmwIFPWNUBk\WyuczDjc{11emGwc3\lZ5Rm\CC5aYToJGdidDRiYX\0[ZIhPDBiaILzJIJ6KGy3Y3nm[ZJie2ViYnHz[YQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6xJO69VS5? NEHoVWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxOVA3Oyd-MkG1NVUxPjN:L3G+
3T3L1 NFTHe|dHfW6ldHnvckBie3OjeR?= M{nXfVExKHWP MUfJcoNz\WG|ZTDpckBi\Gmyb37lZ5RqdiCvUl7BJIxmfmWuczDpckBVVk[jbIDoZU1qdmS3Y3XkJI1wfXOnIEPUN2wyKGOnbHzzJJBz\XS{ZXH0[YQh[XRiMUCgeW0h[mWob4LlJHRPTi2jbIDoZUBkcGGubHXu[4UhemWuYYTpeoUhfG9iY3;ueJJwdA>? MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh3MUC4PUc,OjF6NUGwPFk9N2F-
COS7 MXnGeY5kfGmxbjDhd5NigQ>? NGf2bmlOd2S3bHH0bY9vKG:oIHj1cYFvKFCSQWLnZY1u[S2OQlSg[ZhxemW|c3XkJIlvKGGocnnjZY4h\3KnZX6gcY9vc2W7IFPPV|ch[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBI[Wx2IHHzd4V{e2WmIHHzJIFkfGm4YYTpc44hd2ZidILhcpNi[3SrdnH0bY9vKGGldHn2bZR6KGK7IHz1Z4ln\XKjc3WgZZN{[XluIFXDOVAhRSByLkC0N{DPxE1w MorLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{OwO|AoRjJzOEezNFcxRC:jPh?=
Sf21 NGrsUXVHfW6ldHnvckBie3OjeR?= NEfIeXZKdmirYnn0bY9vKG:oIHj1cYFvKEKVRWCg[ZhxemW|c3XkJIlvKHCuYYPtZUBu\W2kcnHu[UB3\XOrY3zld{Bw\iCVZkKxJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiQWTQMYRmeGWwZHXueEBcO0ifdHH1do9kcG:uYYTlJJVxfGGtZTygTWM2OCB;IE[uOEDPxE1w M3XOUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOU[1OlI{Lz5{MUm2OVYzOzxxYU6=
Sf21 NFq3[FlHfW6ldHnvckBie3OjeR?= NXf3d2JOUW6qaXLpeIlwdiCxZjDTdJJi\3WnLVThe4xmgSC{YYSgRpNmeCCneIDy[ZN{\WRiaX6gdIxie22jIH3lcYJz[W6nII\ld4lkdGW|IH;mJHNnOjFiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBCXFBvZHXw[Y5l\W62IGuzTH11[XW{b3Poc4xifGVidYD0ZYtmNCCLQ{WwJF0hPDBizszNMi=> Moi1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NkW2NlMoRjJzOU[1OlI{RC:jPh?=
COS-1 MnTZSpVv[3Srb36gZZN{[Xl? NIPmT4szPCCqcoO= NHzkcY5C\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJGNQWy1zIHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWtZoF{\WRibIXtbY5wdWW2cnnjJIFv[Wy7c3nzMEBGSzVyIE2gNE4xPDhizszNMi=> M3LRVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEWxNFU1Lz5{MkC1NVA2PDxxYU6=
COS1 MYLGeY5kfGmxbjDhd5NigQ>? MoDZRolv\GmwZzDh[oZqdmm2eTD0c{BpfW2jbjD3bYxlKHS7cHWgVHBCWmejbX3hJGxDTCCneIDy[ZN{\WRiaX6gR29UOSClZXzsd{Bkdy2neIDy[ZN{cW6pIFfBUFQh[nlic3PpcpRqdGyjdHnvckBxem:6aX3peJkh[XO|YYm= MkXxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyN{C2NFQoRjJ{MEewOlA1RC:jPh?=
HepG2 M{nISWZ2dmO2aX;uJIF{e2G7 NVG1d5Y1OjBiaILz NWXUbHhrSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBISUx2LX\1d4VlKFCSQWLnZY1u[SCuaXfhcoQh[mmwZHnu[{Bld22jaX6g[ZhxemW|c3XkJIlvKEincFeyJINmdGy|IHHmeIVzKDJyIHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6wNkDPxE1w MnLoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyOEG5N|IoRjJ{MEixPVMzRC:jPh?=
BL21 DE3 M2fSZ2Z2dmO2aX;uJIF{e2G7 NXT2fVJlTGm|cHzhZ4Vu\W62IH;mJHs{UF2{b4Pp[4xqfGG8b37lJIZzd21iTj30[ZJucW6jbDDIbZMufGGpZ3XkJIh2dWGwIGDQRXJo[W2vYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQlyyNUBFTTNiY3XscJMh[nlic3PpcpRqdGyjdHnvckBxem:6aX3peJkh[XO|YYmsJGtqKD1iMD6wO|Qh|ryPLh?= NFnaO5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC4NVk{Oid-MkKwPFE6OzJ:L3G+
COS1 M1nvUmZ2dmO2aX;uJIF{e2G7 M3HnNVI1KGi{cx?= MlfNWJJidnOjY4TpeoF1cW:wIH;mJIh2dWGwIH\1cIwhdGWwZ4ToJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iQ1;TNUBk\WyuczDjc{11emGwc3\lZ5Rm\CC5aYToJHJZWmGucHjhJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjF{IN88UU4> M{LCflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUm3N|k3Lz5{MkG5O|M6PjxxYU6=
MG-63 NUGxT3FITnWwY4Tpc44h[XO|YYm= MoDPRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW|c3XkJIlvKE2JLU[zJINmdGy|IHL5JJJmeG:{dHXyJIdmdmVvYnHz[YQhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6wNVEh|ryPLh?= M2PUN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkK1OlQyLz5{MkKyOVY1OTxxYU6=
293H DA M2nVe2Z2dmO2aX;uJIF{e2G7 MWexOkBpenN? NWfGbJFpSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiTFLEJIlvKGi3bXHuJFI6O0hiRFGgZ4VtdHNiYX\0[ZIhOTZiaILzJIJ6KFSULV\SSXQh[WO2aY\heIlwdiC{ZYDvdpRmeiCjc4PhfUwhTUN3MDC9JFAvODB{NDFOwG0v NIPSWIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW4Nlk4Oyd-MkK1PFI6PzN:L3G+
MG-63 NXfwfIh{TnWwY4Tpc44h[XO|YYm= NUDmWoRpOjRiaILz NIPSZlNOd2S3bHH0bY9vKG:oIH\1cIwudGWwZ4ToJIh2dWGwIIDTS|Uu\nW|ZXSgVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCPRz22N{Bk\WyuczDjc{1mgHC{ZYPzbY5oKHCJVj3QNk1RWFKHIHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[mG|ZXSgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMUGg{txONg>? NYnJU3cyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3Nlc1PDhpPkKyO|I4PDR6PD;hQi=>
Ac2F NV7z[pZ5TnWwY4Tpc44h[XO|YYm= MmL1NE4yKHSxIEGwJJVO MlvZOkBpenN? MX;B[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiC{YYSgRYMzTiClZXzsd{Bie3Onc4Pl[EBieyCudXPp[oVz[XOnIHHjeIl3cXS7IHH0JFAvOSC2bzCxNEB2VSCjZoTldkA3KGi{czDifUBz\XCxcoTldkBo\W6nIHHzd4F6 MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjhzOUG5NEc,OjJ6MUmxPVA9N2F-
HepG2 NFHpRlZHfW6ldHnvckBie3OjeR?= MmXDWJJidnOjY4TpeoF1cW:wIH;mJGdCVDRvZoXz[YQhWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjD0doFve2[nY4Tl[EBqdiCqdX3hckBJ\XCJMjDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjFizszNMi=> NWj4ZmZZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5N|Q2OzdpPkKyPVM1PTN5PD;hQi=>
THP1 MlrBSpVv[3Srb36gZZN{[Xl? MWqxNFAhfU1? NYn6VFJ{OyCqcoO= NWXVO3V1XHKjboPhZ5RqfmG2aX;uJI9nKFCSQWLnZY1u[SCrbjDoeY1idiCWSGCxJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKEySUz3pcoR2[2WmIF3DVFEhdVKQQTDlfJBz\XO|aX;uJIF1KDFyMDD1UUBxemWrbnP1ZoF1\WRiZn;yJFMhcHK|IIDybY9zKHSxIFzQV{BkcGGubHXu[4UhdWWjc4Xy[YQh[W[2ZYKgNVghcHK|IHL5JHJVNVCFUjDhcoFtgXOrczDy[YxifGm4ZTD0c{B2dnS{ZXH0[YQh[2:wdILvcC=> MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl|NEWzO{c,OjJ7M{S1N|c9N2F-
THP1 MmTrSpVv[3Srb36gZZN{[Xl? Mn3ZNVAxKHWP MWqzJIhzew>? M3vWeXRz[W6|YXP0bZZifGmxbjDv[kBRWEGUZ3HtcYEhcW5iaIXtZY4hXEiSMTDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckBNWFNvaX7keYNm\CCLTE[gcXJPSSCneIDy[ZN{cW:wIHH0JFExOCC3TTDwdoVqdmO3YnH0[YQh\m:{IEOgbJJ{KHC{aX;yJJRwKEySUzDjbIFtdGWwZ3WgcYVie3W{ZXSgZYZ1\XJiMUigbJJ{KGK7IGLUMXBEWiCjbnHsfZNqeyC{ZXzheIl3\SC2bzD1cpRz\WG2ZXSgZ49vfHKxbB?= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl|NEWzO{c,OjJ7M{S1N|c9N2F-
HepG2 NVzaRW91TnWwY4Tpc44h[XO|YYm= M1fUcVExKHWP M3SxcFI1KGi{cx?= NYC4eHlHWmWmdXP0bY9vKG:oIHfseYNwe2ViY3;ud5VueHSrb36gbY4hcW6|dXzpck1z\XOrc4ThcpQhcHWvYX6gTIVxTzJiY3XscJMh[XRiMUCgeW0h[W[2ZYKgNlQhcHK|IHL5JIdtfWOxc3Wgc5hq\GG|ZTDt[ZRpd2RiaX6gdJJme2WwY3Wgc4YhOC5zIIXNJI9nKGmwc4XsbY4> M124[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEK1NlQ1Lz5{M{CyOVI1PDxxYU6=
HepG2 MmrYSpVv[3Srb36gZZN{[Xl? MYexNEB2VQ>? NEe0XmQzPCCqcoO= M{S5[nJm\HWldHnvckBw\iCpbIXjc5NmKGOxboP1cZB1cW:wIHnuJIlve3WuaX6tdoV{cXO2YX70JIh2dWGwIFjldGczKGOnbHzzJIF1KDFyIIXNJIFnfGW{IEK0JIhzeyCkeTDncJVkd3OnIH;4bYRie2VibXX0bI9lKGmwIIDy[ZNmdmOnIH;mJFIzNjJibV2gc4Yh\2y3Y3;z[S=> NH;n[lQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CyOVI1PCd-MkOwNlUzPDR:L3G+
HepG2 NILadJZHfW6ldHnvckBie3OjeR?= MoCxNlAhcHK| M1z1U3Rz[W6|YXP0bZZifGmxbjDv[kBISUx2LX\1d4VlKGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJGhmeEd{IHPlcIx{KGGodHXyJFIxKGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCzPUDPxE1w NUf6XJJoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxO|ExPDVpPkKzNVcyODR3PD;hQi=>
L02 Mlu3SpVv[3Srb36gZZN{[Xl? MlvQNlQhcHK| MlT5RYdwdmm|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEuVEKGIHX4dJJme3OnZDDpckBpfW2jbjDMNFIh[2WubIOgZ48u\XiycnXzd4lv\yCyR1yzMXNXPDBvR1HMOEBi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHLhd4VlKHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OCB;IECuPUDPxE1w M3\CdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MUi2N|A4Lz5{M{G4OlMxPzxxYU6=
HEK293 MojTSpVv[3Srb36gZZN{[Xl? NEi4bGQ1QCCqcoO= M3Ttd2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZ491emGwc3\lZ5Rm\CC5aYToJHBRWkW6ND3UT{1tfWNiYYPz[ZN{\WRiYYOgZYN1cX[jdHnvckBw\iCudXPp[oVz[XOnIHHjeIl3cXS7IH3lZZN2emWmIHHmeIVzKDR6IHjyd{BjgSC2cnHud4FkfGm4YYTpc44h[XO|YYmsJGVEPTBiPTC0N{44OSEQvF2u NF[yOng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K2OVg1PCd-MkOyOlU5PDR:L3G+
HEK293 MW\GeY5kfGmxbjDhd5NigQ>? M1ntPFExKHWP NEPDcYlVemGwc3HjeIl3[XSrb36gc4YhWFCDUnfhcY1iKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKEGSMj3QVHJGKGG2IEGwJJVOKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> NUn3N3NwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyO|M2OTlpPkKzNlc{PTF7PD;hQi=>
HEK293 MkXJSpVv[3Srb36gZZN{[Xl? M{TVcGFod26rc4SgZZQhcHWvYX6gVHBCWmejbX3hJGxDTCCneIDy[ZN{\WRiaX6gbJVu[W5iSFXLNlk{KGOnbHzzJINwfHKjboPm[YN1\WRid3n0bEBISUx2LVz1Z{Bie3Onc4Pl[EBieyC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjByNDFOwG0v M{nIN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Mki2O|g4Lz5{M{K4Olc5PzxxYU6=
HEK293 NFXHPY5HfW6ldHnvckBie3OjeR?= MVOyOEBpenN? MkHIWJJidnOjY4TpeoF1cW:wIH;mJGdCVDRiRFLEMYZ2e2WmIHj1cYFvKFCSQWLnZY1u[S2OQlSg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4yKM7:TT6= Mkj4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OUS4N|AoRjJ|Mkm0PFMxRC:jPh?=
CMT93 Mn;MSpVv[3Srb36gZZN{[Xl? M3T3ZVExKHWP NFvPeIw{OCCvaX7z MXjJcoR2[3Srb36gc4YhWFCDUj3nZY1u[SCycn;tc5RmeiCjY4Tpeol1gSCrbjDMVHMue3SrbYXsZZRm\CCvb4Xz[UBEVVR7MzDj[YxteyCjdDCxNEB2VSCycnXpcoN2[mG2ZXSg[o9zKDNyIH3pcpMh[mWob4LlJGxRWy2laHHscIVv\2VibXXhd5Vz\WRiYX\0[ZIhPiCqcoOgdI9{fCClaHHscIVv\2ViYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 NH;pPVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SxOlE6OCd-MkO0NVYyQTB:L3G+
HepG2 MUDGeY5kfGmxbjDhd5NigQ>? NV30UoM2SWexbnnzeEBi[3Srdnn0fUBifCCJQVy0MYZ2e2WmIGDQRXJo[W2vYTDNOFY{SSCvdYThcpQhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiYomgeJJidnOjY4TpeoF1cW:wIHHzd4F6NCCHQ{WwJF0hOC5yMEKg{txONg>? MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzVyMkKxNkc,OjN3MEKyNVI9N2F-
HepG2 NHPCNolHfW6ldHnvckBie3OjeR?= MV3B[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRvZoXz[YQhWFCDUnfhcY1iKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIHj1cYFvKEincFeyJINmdGy|IHL5JJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMEGg{txONg>? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzVyMkKxNkc,OjN3MEKyNVI9N2F-
L6 MXfGeY5kfGmxbjDhd5NigQ>? MVmyOEBpenN? NFTye5BKdmS3Y4Tpc44hd2ZiZ3z1Z49{\SC3cIThb4UhcW5icnH0JGw3KGOnbHzzJJB2dHOnZDD3bZRpKENzND3k[Y95gSCpbIXjc5NmKGGodHXyJFI1KGi{czDpckBxemW|ZX7j[UBw\iCrboP1cIlvNCCHQ{WwJF0hPC52OTFOwG0v NUW1TYNRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5PVI5PjJpPkKzPVkzQDZ{PD;hQi=>
L6 M1f5cGZ2dmO2aX;uJIF{e2G7 NYfFOVg4OTZiaILz NH7OSllKdmO{ZXHz[UBqdiB{LWuzTH0u\GWxeInncJVkd3OnIIXweIFs\SCrbjDyZZQhVDZiY3XscJMh[W[2ZYKgNVYhcHK|IHL5JJNkcW62aXzsZZRqd25iY3;1cpRqdmdiYX7hcJl{cXNuIFnDOVAhRSB2Lkig{txONg>? MmjaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUO3N|goRjJ2OEGzO|M5RC:jPh?=
HEK293 NWHENZR1TnWwY4Tpc44h[XO|YYm= M1zhfVExKHWP NX7UdHlpOjRiaILz M1zpTHRz[W6|YXP0bZZifGmxbjDv[kBRWEGULXfhcY1iKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{BifCBzMDD1UUBi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 NI\XTHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi5NFA6OCd-MkS4PVAxQTB:L3G+
HEK293 M{jjOmZ2dmO2aX;uJIF{e2G7 M{m5W|I1KGi{cx?= MU\B[49vcXO2IHHjeIl3cXS7IHH0JI1wfXOnIGDQRXJo[W2vYTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHPvMYV5eHKnc4Ppcochf2m2aDDHZYw1KHKncH;yeIVzKH[nY4TvdkBi\nSncjCyOEBpenNiYomg[JVidC2udXPp[oVz[XOnIILldI9zfGW{IHHzd4F6NCCHQ{WwJF0hOC5yMzFOwG0v MkH5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NUW4PFkoRjJ2OUW1PFg6RC:jPh?=
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MDA-MB-231 NYXX[ph5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnPUNlQhcHK| NWDGPVJtT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gOU4zOyEQvF2u M4[4XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mke4NlM3Lz5{NUK3PFI{PjxxYU6=
HEK293 M4TQemZ2dmO2aX;uJIF{e2G7 MnPFRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDQVGFT\2GvbXGg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkCxJO69VS5? M37CblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C1Olg5Lz5{NUOwOVY5QDxxYU6=
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HEK293 MkjvSpVv[3Srb36gZZN{[Xl? MmjpNlQhcHK| NV35[JVYXHKjboPhZ5RqfmG2aX;uJI9nKFCSQWLnZY1u[SBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMhcW6ldXLheIVlKG[xcjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 M3zvfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NESyN|IzLz5{NUS0NlMzOjxxYU6=
HepG2 MXjGeY5kfGmxbjDhd5NigQ>? MXfB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFfBUFQuWFCDUnfhcY1iKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO|ZXSgbY4hcHWvYX6gTIVxTzJiY3XscJMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gNE4xOzlizszNMi=> MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MkK4NUc,OjV2NkKyPFE9N2F-
HepG2 MV;GeY5kfGmxbjDhd5NigQ>? M{SwTFIxKGi{cx?= MWLB[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRvRF7BJIJqdmSrbneg[I9u[WmwIH\1d4VlKGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJIh2dWGwIFjldGczKGOnbHzzJIF{e2W|c3XkJIF{KHKnY3XweI9zKHS{YX7zZYN1cX[jdHnvckBqdmO3YnH0[YQh\m:{IEKwJIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjB2IN88UU4> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR7N{GzNkc,OjV2OUexN|I9N2F-
HepaR M3Ty[WZ2dmO2aX;uJIF{e2G7 NF:1emEyKHWP NEj4TXEyKGSjeR?= MYLB[49vcXO2IHHjeIl3cXS7IHH0JHBRSVKpYX3tZUBqdiCqdX3hckBJ\XCjUjDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBHSUKSMTDn[Y5mKGW6cILld5Nqd25iYYSgNUB2VSCrbnP1ZoF1\WRiZn;yJFEh\GG7IHL5JJF2[W62aYTheIl3\SCSQ2KgcYV1cG:mIILlcIF1cX[nIITvJJVvfHKnYYTl[EBkd262cn;s MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR7N{GzNkc,OjV2OUexN|I9N2F-
HepaR MYjGeY5kfGmxbjDhd5NigQ>? M1rOTVEhfU1? M3TQcVEh\GG7 NV7KcIFoSWexbnnzeEBi[3Srdnn0fUBifCCSUFHS[4FudWFiaX6gbJVu[W5iSHXwZXIh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iQV7HVHRNPCCpZX7lJIV5eHKnc4Ppc44h[XRiMTD1UUBqdmO3YnH0[YQh\m:{IEGg[IF6KGK7IIH1ZY51cXSjdHn2[UBRS1JibXX0bI9lKHKnbHH0bZZmKHSxIIXueJJm[XSnZDDjc451em:u NV\Sfmw4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PVcyOzJpPkK1OFk4OTN{PD;hQi=>
HepaR M3zPeWZ2dmO2aX;uJIF{e2G7 M2X0WVEhfU1? M4TBRVEh\GG7 MmTWRYdwdmm|dDDhZ5Rqfmm2eTDheEBRWEGUZ3HtcYEhcW5iaIXtZY4hUGWyYWKgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hUE2JQ2OyJIdmdmViZYjwdoV{e2mxbjDheEAyKHWPIHnuZ5Vj[XSnZDDmc5IhOSCmYYmgZpkheXWjboTpeIF1cX[nIGDDVkBu\XSqb3SgdoVt[XSrdnWgeI8hfW62cnXheIVlKGOxboTyc4w> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR7N{GzNkc,OjV2OUexN|I9N2F-
C2C12 M{TjOmZ2dmO2aX;uJIF{e2G7 Ml[4N|AhfU1? M4jTU|IhcHK| Mn;1TY5lfWO2aX;uJI9nKDJvZHXvfJkuYzOKXT3ncJVkd3OnIIXweIFs\SCrbjDtc5V{\SCFMlOxNkBk\WyuczDheEA{OCC3TTDh[pRmeiB{IHjyd{BjgSCuaYH1bYQhe2OrboTpcIxifGmxbjDjc5VvfGmwZzDhcoFtgXOrcx?= NWT5UpI5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4N|U2OzdpPkK1PFM2PTN5PD;hQi=>
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HEK293 NYLLV3dVTnWwY4Tpc44h[XO|YYm= M1W3cVI1KGi{cx?= MUHB[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRvdHHn[4VlKGi3bXHuJHBRSVKpYX3tZUBtcWejbnSgZolv\GmwZzDkc41icW5iY3jpcYVzcWNicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxKD1iMD6yOkDPxE1w MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzV7MUCwOkc,Ojd3OUGwNFY9N2F-
HepG2 NYXwTmhoTnWwY4Tpc44h[XO|YYm= MYqyNEBpenN? MkHmWJJidnOjY4TpeoF1cW:wIH;mJGdCVDRvZoXz[YQhcHWvYX6gVHBCWmejbX3hJGxDTCCneIDy[ZN{\WRiaX6gbJVu[W5iSHXwS|Ih[2WubIOgZYZ1\XJiMkCgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODRizszNMi=> MmHiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyN{[4NlcoRjJ6MEe2PFI4RC:jPh?=
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HepG2 NEDUT4ZHfW6ldHnvckBie3OjeR?= M4\L[VIxKGi{cx?= MXHUdoFve2GldHn2ZZRqd25ib3[gS2FNPC22YXfn[YQhcHWvYX6gVHBCWmejbX3hJGxDTCCneIDy[ZN{\WRiaX6gbJVu[W5iSHXwS|Ih[2WubIOgZYZ1\XJiMkCgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFAvODN7IN88UU4> MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODF7OUK1N{c,OzBzOUmyOVM9N2F-
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3T3L1 NGX3[HVHfW6ldHnvckBie3OjeR?= NW\oOpJ4OiC3TR?= MmrYTY5lfWO2aX;uJI9nKGyrcHnkJIFk[3WvdXzheIlwdiCrbjDtc5V{\SB|VEPMNUBk\WyuczDheEAzKHWPIHL5JG9qdCCUZXSgU{B{fGGrbnnu[{1j[XOnZDDhd5NigQ>? MkPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzOUmyOVMoRjNyMUm5NlU{RC:jPh?=
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COS7 MkLGSpVv[3Srb36gZZN{[Xl? MoXGNlQhcHK| NVWyS4E4SWexbnnzeEBi[3Srdnn0fUBifCC{ZXPvcYJqdmGwdDDoeY1idiCJQVy0MWRDTCCodYPl[EBRWEGUZ3HtcYEhVEKGIHX4dJJme3OnZDDpckBEV1N5IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkO5OkDPxE1w NETKVGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEKyNFYxOid-M{CyNlA3ODJ:L3G+
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3T3L1 M{j4V2Z2dmO2aX;uJIF{e2G7 MUG3JIRigXN? M4jZNXVxemWpdXzheIlwdiCxZjDGRXMheHKxdHXpckBmgHC{ZYPzbY9vKGmwIHTp[oZmemWwdHnheIVlKG2xdYPlJFNVO0xzIHPlcIx{KGGodHXyJFch\GG7czDifUB4\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlEwLz6FaFXNRmw9N2F-
3T3L1 M{njeWZ2dmO2aX;uJIF{e2G7 NFXBUlI4KGSjeYO= MnHyWZBz\We3bHH0bY9vKG:oIHHQNkBxem:2ZXnuJIV5eHKnc4Ppc44hcW5iZHnm[oVz\W62aXH0[YQhdW:3c3WgN3Q{VDFiY3XscJMh[W[2ZYKgO{Bl[Xm|IHL5JJdme3Sncn6gZoxwfCCjbnHsfZNqew>? NGLVbIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjFxJ{7DbGVOSkx:L3G+
3T3L1 MorNSpVv[3Srb36gZZN{[Xl? NWfreo95OjRiaILz NW\GUHJ4UW6qaXLpeIlwdiCxZjDjbJJwdmmlIHnud5VtcW5vc4TpcZVt[XSnZDDpcpN2dGmwIILld4l{fGGwY3WgbY4h\GmoZnXy[Y51cWG2ZXSgcY92e2ViM2SzUFEh[2WubIOgZZN{\XO|ZXSgZZMhOi2mZX;4fU1FNVt|SG2t[4x2[2:|ZTD1dJRic2ViYX\0[ZIhOjRiaILzJIJ6KHOlaX70bYxt[XSrb36gZ492dnSrbnegZY5idHm|aYO= M13iZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzOS9pPlPoSW1DVDxxYU6=
3T3L1 NEOzSVZHfW6ldHnvckBie3OjeR?= MoTZNVAhfU1? M3HOcFI1KGi{cx?= MVzJcoR2[3Srb36gc4YhcW6|dXzpck1{fGmvdXzheIVlKDJvZHXvfJkuTC2dM1jdMYdtfWOxc3WgeZB1[WunIHnuJIRq\m[ncnXueIlifGWmIH3veZNmKDOWM1yxJINmdGy|IHH0JFExKHWPIHHmeIVzKDJ2IHjyd{BjgSC|Y3nueIltdGG2aX;uJINwfW62aX7nJIFv[Wy7c3nz NV\hVYRpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJzLze+R4hGVUKOPD;hQi=>
3T3L1 M3jXZ2Z2dmO2aX;uJIF{e2G7 MViyJJVO NHjzTWYyPCCmYYnz MkPDRYdwdmm|dDDhZ5Rqfmm2eTDheEBRWEGUIHnuJI1wfXOnIEPUN2wyKGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHHkbZBw[3m2ZTDkbYZn\XKnboTpZZRqd25iYYSgNkB2VSCjZoTldkAyPCCmYYnzJIJ6KE:rbDDS[YQhVyC|dHHpcolv\yCvZYToc4Q> NYjXPHE1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJzLze+R4hGVUKOPD;hQi=>
U2OS MmnvSpVv[3Srb36gZZN{[Xl? NHr4V40yOCC3TR?= MYeyOEBpenN? MkT6RYN1cX[jdHnvckBw\iCSUFHSS{ApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCqdX3hckBWOk:VIHPlcIx{KGG2IEGwJJVOKGmwY4XiZZRm\CCob4KgNlQhcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigQ>? NI\0dFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjFxJ{7DbGVOSkx:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cyclin D1 / CDK2 / CDK4 / Bax / Bcl-2 ; 

PubMed: 29725405     


A431 cells at 80% confluence were stimulated with rosiglitazone for 24 h, and the effects on Bax, Bcl-2, cyclin D1, cdk2 and cdk4 expression were analyzed by western blotting. Data were quantified by scanning densitometry, and the ratio of the protein of interest was normalized to GAPDH. Data are presented as means ± SEM (n=3). #P<0.05 (10, 20, 30, 40 mM rosiglitazone treatment compared with control treatment for cyclin D1/GAPDH and Cdk4/GAPDH; 30, 40 mM rosiglitazone treatment compared with control treatment for Cdk2/GAPDH) as analyzed with one-way ANOVA. 

29725405
Immunofluorescence
PPARγ / E-cadherin ; 

PubMed: 27145370     


Increased PPARγ (green) and E-cadherin (red) with rosiglitazone treatment and overlaid image showing colocalization (yellow, arrows) in high glucose condition (30 mM).

27145370
Growth inhibition assay
Cell viability ; 

PubMed: 29725405     


MTS assay of A431 cell viability. A431 cells were incubated with different concentrations (0–100 µM) rosiglitazone for 24 h. Cell viability was determined by MTS assay kit. Data are presented as the mean ± standard deviation of three independent experiments. #P<0.05 (0 µM rosiglitazone vs. 100 µM rosiglitazone) as analyzed with one-way analysis of variance.

29725405
In vivo

Rosiglitazone administration results in significant bone loss, including a decrease in bone volume, trabecular width, and trabecular number and an increase in trabecular spacing. Rosiglitazone also leads to a decrease in bone formation rate, with a simultaneous increase in fat content in the bone marrow. Rosiglitazone decreases the expression of the osteoblast-specific genes Runx2/Cbfa1, Dlx5, and alpha1(I)collagen, whereas the expression of the adipocyte-specific fatty acid binding protein aP2 is increased. [2] Rosiglitazone upregulates gene transcripts encoding mitochondrial proteins in white adipocytes from ob/ob mice accompanied by an increase in mitochondrial mass and changes in mitochondrial structure. [4]

Protocol

Solubility (25°C)

In vitro DMSO 71 mg/mL (198.64 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 357.43
Formula

C18H19N3O3S

CAS No. 122320-73-4
Storage powder
in solvent
Synonyms N/A
Smiles CN(CCOC1=CC=C(C=C1)CC2C(=O)NC(=O)S2)C3=CC=CC=N3

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01100619 Completed Drug: rosiglitazone|Drug: XL184 Papillary Thyroid Cancer|Follicular Thyroid Cancer|Huerthle Cell Thyroid Cancer|Renal Cell Carcinoma Exelixis April 2010 Phase 1
NCT00609362 Completed Drug: Rosiglitazone|Drug: Placebo pill Change in Bone Mineral Density|Change in Bone Marrow Fat Content University of Aarhus January 2008 Phase 2
NCT00405015 Completed Drug: rosiglitazone Ischemia-Reperfusion Injury|The Metabolic Syndrome Radboud University|GlaxoSmithKline April 2007 Phase 2
NCT00486187 Completed Drug: rosiglitazone|Drug: metformin or sulfonylurea Type 2 Diabetes Mellitus Dr. Milan Gupta|GlaxoSmithKline|Canadian Collaborative Research Network April 2006 Not Applicable
NCT00370305 Completed Drug: rosiglitazone Metabolic Syndrome|Impaired Glucose Tolerance Charite University Berlin Germany May 2004 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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PPAR Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID