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Eupatilin PPAR agonist

Name: Eupatilin
Brand: Selleck Chemicals
SKU: S3846
Availability: InStock
Rating: 5 (4 reviews)

Cat.No.S3846

Eupatilin (NSC 122413), a major flavonoid from Artemisia plants, possesses various beneficial biological effects including anti-inflammation, anti-tumor, anti-cancer, anti-allergy, and anti-oxidation activity. This compound, a lipophilic flavonoid isolated from Artemisia species, is a PPARα agonist, and possesses anti-apoptotic, anti-oxidative and anti-inflammatory activities.

Chemical Structure

Molecular Weight: 344.32

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Quality Control

Batch: Purity: 99.45%

99.45

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase Vitamin Carbohydrate Metabolism Mitochondrial Metabolism Drug Metabolite
Other PPAR Inhibitors	T0070907 GW9662 GW6471 WY-14643 (Pirinixic Acid) GSK3787 GW0742 AZ6102 Astaxanthin GSK0660 Oroxin A

Solubility

In vitro
Batch:

DMSO : 68 mg/mL (197.49 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.450mg/ml (10.02mM)	Taking the 1 mL working solution as an example, add 50 μL of 69 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.850mg/ml (2.47mM)	Taking the 1 mL working solution as an example, add 50 μL of 17 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	344.32	Formula	C₁₈H₁₆O₇	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	22368-21-4	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	NSC 122413			Smiles	COC1=C(C=C(C=C1)C2=CC(=O)C3=C(O2)C=C(C(=C3O)OC)O)OC

Mechanism of Action

In vitro	Eupatilin has been reported to induce apoptosis in human gastric cancer AGS cells, also triggers differentiation of these cells. Treatment of AGS cells with this compound induces cell cycle arrest at the G1 phase with the concomitant induction of p21^cip1, a cell cycle inhibitor. It also markedly induces trefoil factor1(TFF1). This compound dramatically induces redistribution of tight junction proteins such as occludin and ZO-1, and F-actin at the junctional region between cells. It also induces phosphorylation of extracellular signal-regulated kinase 2 and p38 kinase. This chemical is known to inhibit the growth of MCF-10A-ras cells by inhibiting the expression of cell cycle regulators such as cyclinD1, cyclinB1, Cdk2, and Cdc2.
In vivo	Treatment with eupatilin significantly decreases serum alanine aminotransferase and serum aspartate aminotransferase as well as liver histologic changes. This compound also preventes hepatic glutathione depletion and increases malondialdehyde levels induced by Ischemia-reperfusion injury (IRI). It improves the acute hepatic IRI by reducing inflammation and apoptosis. Oral administration of this chemical (10 mg/kg) in a therapeutic paradigm significantly reduces brain infarction and improves neurological functions in tMCAO-challenged mice. Its administration reduces the number of Iba1-immunopositive cells across ischemic brain and induces their morphological changes from amoeboid into ramified in the ischemic core, which is accompanied with reduced microglial proliferation in ischemic brain. This agent suppresses NF-κB signaling activities in ischemic brain by reducing IKKα/β phosphorylation, IκBα phosphorylation, and IκBα degradation.
References	[1] https://pubmed.ncbi.nlm.nih.gov/19281788/ [2] https://pubmed.ncbi.nlm.nih.gov/27320593/ [3] https://pubmed.ncbi.nlm.nih.gov/28178289/

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