Glabridin

For research use only.

Catalog No.S3786 Synonyms: Q-100692, KB-289522, LS-176045

1 publication

Glabridin Chemical Structure

CAS No. 59870-68-7

Glabridin (Q-100692, KB-289522, LS-176045), one of the active phytochemicals in licorice extract, binds to and activates the ligand binding domain of PPARγ, as well as the full length receptor. It is also a GABAA receptor positive modulator promoting fatty acid oxidation and improving learning and memory.

Selleck's Glabridin has been cited by 1 publication

Purity & Quality Control

Choose Selective PPAR Inhibitors

Biological Activity

Description Glabridin (Q-100692, KB-289522, LS-176045), one of the active phytochemicals in licorice extract, binds to and activates the ligand binding domain of PPARγ, as well as the full length receptor. It is also a GABAA receptor positive modulator promoting fatty acid oxidation and improving learning and memory.
Targets
PPARγ [1]
(Cell-free assay)
α1β2γ2 (GABAA receptor) [3]
()
α1β3γ2 (GABAA receptor) [3]
()
α1β2 (GABAA receptor) [3]
()
α1β1γ2 (GABAA receptor) [3]
()
6.3 μM(EC50) 6.83 μM(EC50) 9.63 μM(EC50) 17.23 μM(EC50)
In vitro

Glabridin induces dose-dependent increase in estrogenic activity and cell proliferation in Ishikawa cells. Glabridin exerts estrogenic activity via the ER-α-SRC-1-co-activator complex[1]. Glabridin has substantial neuroprotective effects, as indicated by the reduced cell death, apoptosis, DNA fragmentation, probably through the regulation of bcl-2, Bax and caspase-3 which are all closely related to cell apoptotic process[2].

In vivo Glabridin at 25mg/kg by intraperitoneal injection, but not at 5mg/kg, significantly decreases the focal infarct volume, cerebral histological damage and apoptosis in MCAO rats compared to sham-operated rats. Glabridin significantly attenuates the level of brain malonyldialdehyde (MDA) in MCAO rats, while it elevates the level of two endogenous antioxidants in the brain, i.e. superoxide dismutase (SOD) and reduced glutathione (GSH). Co-treatment with glabridin significantly inhibits the staurosporine-induced cytotoxicity and apoptosis of cultured rat cortical neurons in a concentration-dependent manner. Consistently, glabridin significantly reduces the DNA laddering caused by staurosporine in a concentration-dependent manner. Glabridin has a neuroprotective effect via modulation of multiple pathways associated with apoptosis. Glabridin appears to achieve steady state concentrations slowly in humans. The limited penetration of glabridin into the brain due to the activity of P-glycoprotein in the blood-brain barrier may also lessen the activity of glabridin at low doses[2].

Protocol

Cell Research:[1]
- Collapse
  • Cell lines: Ishikawa cells
  • Concentrations: 1nM to 10μM
  • Incubation Time: 72 h
  • Method: The effect of the extracts on cell proliferation are estimated using MTT assay. Briefly, 10μL of 5 mg/ml MTT is added to each well after incubation with test compounds for 72 h. DMSO is then added to dissolve the formazan and the plate read at 595 nm. The cell proliferation of control cells is 100.00% and any increase or decrease in cell proliferation of treated cells is compared to the control cells. All experimental conditions are assayed in triplicate.
    (Only for Reference)
Animal Research:[2]
- Collapse
  • Animal Models: Male healthy Sprague-Dawley rats
  • Dosages: 5 mg/kg and 25 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 64 mg/mL (197.3 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 324.37
Formula

C20H20O4

CAS No. 59870-68-7
Storage powder
in solvent
Synonyms Q-100692, KB-289522, LS-176045
Smiles CC1(C=CC2=C(O1)C=CC3=C2OCC(C3)C4=C(C=C(C=C4)O)O)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

PPAR Signaling Pathway Map

Related PPAR Products

Tags: buy Glabridin | Glabridin supplier | purchase Glabridin | Glabridin cost | Glabridin manufacturer | order Glabridin | Glabridin distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID