WY-14643 (Pirinixic Acid)

Catalog No.S8029 Synonyms: NSC 310038

For research use only.

WY-14643 (Pirinixic Acid, NSC 310038) is a potent and selective PPARα activator with an EC50 of 1.5 μM.

WY-14643 (Pirinixic Acid) Chemical Structure

CAS No. 50892-23-4

Selleck's WY-14643 (Pirinixic Acid) has been cited by 13 publications

Purity & Quality Control

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Biological Activity

Description WY-14643 (Pirinixic Acid, NSC 310038) is a potent and selective PPARα activator with an EC50 of 1.5 μM.
Targets
PPARα [1]
1.5 μM(EC50)
In vitro

WY 14643 (10 μM) almost completely inhibits interleukin-1-induced production of interleukin-6 and prostaglandin and expression of cyclooxygenase-2 in aortic smooth-muscle cells, through repression of NF-κB signaling. [2] WY14643 (250 μM) reduces VCAM-1 expression levels significantly, to 52 % of TNF-α-stimulated human endothelial cells. Pretreatment of endothelial cells with WY 14643 (10 μM) before TNF-α stimulation reduces U937 cell adhesion by 50%. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Hep G2 cells NHHDVGtHfW6ldHnvckBie3OjeR?= MXXB[49vcXO2IHHjeIl3cXS7IHH0JI1wfXOnIGDQRXJidHCqYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIHj1cYFvKEincDDHNkBk\WyuczDjc{11emGwc3\lZ5Rm\CC5aYToJGdidDRvRFLEJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OD1yLkC0JO69VQ>? MXixPVA2Ozd5Nh?=
MCF7 cells MYjGeY5kfGmxbjDhd5NigQ>? MXjB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGDQRXJidHCqYTDlfJBz\XO|ZXSgbY4hVUOINzDj[YxteyClbz30doFve2[nY4Tl[EBEWFSLIFTSNU11gXCnIGLFJIFnfGW{IE[gbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2wMlU1OiEQvF2= MWqyOFk{PjJ|Mh?=
U2OS cells NVTaR4gzTnWwY4Tpc44h[XO|YYm= NUfKc5p6SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUYXzwbIEhcW5iVULPV{Bk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVA:OTJizszN M4jr[lE5OzJ7N{Wx
HEK293 cells M4D4eWZ2dmO2aX;uJIF{e2G7 NU[2UW9wSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBRWEGUYXzwbIEh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bkd3S{YX7z[oVkfGWmIIfpeIghWFCURYi0MXRMNWy3YzDhd5Nme3OnZDDhd{Bi[3SrdnH0bY9vKG:oIHz1Z4ln\XKjc3WgZYN1cX[rdImgcYVie3W{ZXSgZYZ1\XJiNEigbJJ{KGK7IITyZY5{[WO2aY\heIlwdiCjc4PhfUwhTUN3ME2yN{4{OyEQvF2= NEHCfmUzOzJ4NUi0OC=>
COS7 cells MofTSpVv[3Srb36gZZN{[Xl? MV7BZ5RqfmG2aX;uJI9nKGi3bXHuJHBRSVKjbIDoZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJGNQWzdiY3XscJMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUO2MlMh|ryP MnL4NlUxOzd7MUS=
EAhy926 cells  MoLKSpVv[3Srb36gZZN{[Xl? NVTkfmoyOTBidV2= M3vtXlEzKGh? NFfCZ3VKdmirYnn0bY9vKG:oIIT1ZoUh\m:{bXH0bY9vKGmwIHj1cYFvKEWDaIm5NlYh[2WubIOgZZQhOTBidV2gdJJmNWmwY4XiZZRm\CCob4KgNVIhcHK|IH3lZZN2emWmIHHmeIVzKDJ2IHjyd{BjgSCyaHHz[U1kd262cnHzeEBucWO{b4Pjc5B6 MojLNVkxOzZ3OUS=
mouse NIH3T3 cells NEXKUoRHfW6ldHnvckBie3OjeR?= NHnIdnMxNjFvMUCg{txO MVnUdoFve2GldHn2ZZRqd25ib3[gcY92e2VicnXjc41jcW6jboSgVHBCWmGucHjhJIV5eHKnc4Pl[EBqdiCvb4Xz[UBPUUh|VEOgZ4VtdHNiYYSgNE4yKHWPIITvJFExKHWPIHL5JHBRWkViYXP0bZZifGmxbjDiZZNm\CCmdXHsJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSC{ZXzheIl3\SC2bzDjc451em:u M1jDTFE6OjN5Mkiz
HepaR cells NHjTXIpHfW6ldHnvckBie3OjeR?= NIK1OG8zPSEQvF2= NHfRUGEyKGSjeR?= Mn\QRYdwdmm|dDDhZ5Rqfmm2eTDheEBRWEGUYXzwbIEhcW5iaIXtZY4hUGWyYWKgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hUE2JQ2OyJIdmdmViZYjwdoV{e2mxbjDheEAzPSC3TTDpcoN2[mG2ZXSg[o9zKDFiZHH5JIJ6KHG3YX70bZRifGm4ZTDQR3IhdWW2aH;kJJJmdGG2aY\lJJRwKHWwdILlZZRm\CClb370do9t MkjNNlU1QTdzM{K=
In vivo WY 14643 (1 mg/kg i.v. bolus) administration at 30 min before left anterior descending occlusion, causes significant reduction in infarct size of ∼44% in rats subjected to regional myocardial ischemia (25 min) and reperfusion (2 h). [4] WY 14643 (3 mg/kg) lowers basal plasma levels of glucose, triglycerides (-16% vs. untreated), and leptin (-52%), and also muscle triglyceride (-34%) and total long-chain acyl-CoAs (LCACoAs) (-41%) in high fat-fed rats. WY14643 substantially reduces visceral fat weight and total liver triglyceride content without increasing body weight gain. WY14643 enhances whole body insulin sensitivity (clamp glucose infusion rate increases 35% and glucose disposals 22%, vs. untreated). WY 14643 enhances insulin-mediated muscle glucose metabolic index (Rg') in red (47%) and white (63%) muscles as well as in white adipose tissue (90%), and reduces muscle triglyceride and LCACoA accumulation. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
For best results, use promptly after mixing.

3.25mg/mL

Chemical Information

Molecular Weight 323.8
Formula

C14H14ClN3O2S

CAS No. 50892-23-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C(=CC=C1)NC2=CC(=NC(=N2)SCC(=O)O)Cl)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO % % Tween 80 % ddH2O
%DMSO %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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