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Catalog No.S2298

9 publications

Fisetin Chemical Structure

CAS No. 528-48-3

Fisetin (Fustel) is a potent sirtuin activating compound (STAC) and an agent that modulates sirtuins.

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Selleck's Fisetin has been cited by 9 publications

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  • Liver pathology and serum markers of liver injury in mice fed control (Ctrl), or ethanol (EtOH), or ethanol combined with fisetin (E/Fisetin) diet for 4 weeks. (A) Light microscopy with hematoxylin and eosin staining shows lipid accumulation (arrows) in the liver of mice after alcohol exposure, which was alleviated by fisetin administration. (B) Plasma alanine aminotransferase (ALT) and (C) aspartate aminotransferase (AST) activities. Infinity ALT and AST reagents were used formeasuring ALT and AST activities. Scale bar: 50 lm. Results are mean±SD (n = 3 to 6). Results for bars that do not share a letter differed significantly among groups (p < 0.05). Significant differences among groups are determined by analysis of variance followed by Tukey’s test.

    Alcohol Clin Exp Res, 2016, 40(10):2076-2084.. Fisetin purchased from Selleck.

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Biological Activity

Description Fisetin (Fustel) is a potent sirtuin activating compound (STAC) and an agent that modulates sirtuins.
Sirtuin [1]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MDA-MB-453 MXrD[YxtKH[rYXLpcIl1gSCjc4PhfS=> NGTXcIcxNCB{MDygOFAtKDZyLDC4NEwh[W6mIEGwNEDDvU1? M4Dvc|I1NCB2ODygZY5lKDd{IHjveZJ{ NGHDcnBYcXSqIHnuZ5Jm[XOrbnegZ49v[2WwdILheIlwdnNib3[g[ol{\XSrbjCoNEwhOjBuIESwMEA3OCxiOECsJIFv\CBzMECgxtVOMSxidHjldoUhf2G|IHGg[IVkemWjc3Wgc4J{\XK4ZXSgbY4h[2WubDDueY1j\XJiaX6g[Iln\mW{ZX70JJRqdWViaX70[ZJ3[Wy|IDiyOEwhPDhuIHHu[EA4OiCqb4Xyd{kv NG[wdJg{ODR|MU[5Ni=>
SGC7901 NH\uS2RRem:uaX\ldoF1cW:wIHHzd4F6 NFnQ[Y4xNCBzLDC1MEAyOCxiMUWgc5IhOjBiwsXN NUPSVZJJPDhiaB?= NVu3SVZ[cW6qaXLpeJMheHKxbHnm[ZJifGmxbh?= MXiyPVgxPTV6MB?=
A549 NELoOGlE\WyuII\pZYJqdGm2eTDhd5NigQ>? MVuwMEAyOCxiMkCsJFMxKG:{IESwJOK2VQ>? NWDZ[YIyOjRuIES4JI9zKDd{IHi= NXzTSmU6\mm|ZYTpckB4[XNiZX\m[YN1cX[nIHnuJIlvcGmkaYTpcochfGinIHfyc5d1cCCxZjDBOVQ6KGOnbHzzJIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> NUjKfIZrOjl2Nke4OVk>
ARPE-19 M3\kSGNmdGxibXnndoF1cW:wIHHzd4F6 NVO1W|ZiPSCxcjCxNEDPxE1? MnTBNlQhcA>? NIj2R3JxemW2cnXheI1mdnRid3n0bEBncXOndHnuJI1iemunZHz5JJJm\HWlZXSgZ4VtdCCvaXfyZZRqd25iaX6gSWdHNXS{ZXH0[YQhSVKSRT2xPUBk\Wyucx?= NVXuZ2lFOjl{OU[wO|A>
SCC-23 M1PMRWNmdGxidnnhZoltcXS7IHHzd4F6 NXvwcVVHOC1zMECg{txO NVnVN5pHOjRiaB?= NWPad|h4\mm|ZYTpckB{cWewaX\pZ4FvfGy7IHnubIljcXSnZDDj[YxtKH[rYXLpcIl1gSCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meiBqc3nncolncWOjboTsfUB4cGWwIITo[UBkd26lZX70doF1cW:wIHnzJIhq\2incjD0bIFvKDJyIN88UUk> MUmyPVMyOjV{MB?=
Tca-8113 M2XBTGNmdGxidnnhZoltcXS7IHHzd4F6 NIDhbFUxNTFyMDFOwG0> NHnv[IIzPCCq Mmf2[ol{\XSrbjDzbYdvcW[rY3HueIx6KGmwaHnibZRm\CClZXzsJJZq[WKrbHn0fUBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldkApe2mpbnnmbYNidnSueTD3bIVvKHSqZTDjc45k\W62cnH0bY9vKGm|IHjp[4hmeiC2aHHuJFIxKM7:TTm= M3f3XFI6OzF{NUKw

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
p-IKKα/β / IKKβ / IKKα / p-IκBα / IκBα / p-p65 / p65 ; 

PubMed: 23861901     

Similar experiments were conducted with increasing doses of fisetin (1 to 10 µM), for a 15 minutes incubation with RANKL. Blots are representative of 3 independent experiments.

XBP1s / ATF6 / CHOP / ATF4 / PERK; 

PubMed: 25016296     

A375 and 451Lu cells were treated with varying doses of fisetin for 24 h and western blot analysis was performed. Equal loading was confirmed by reprobing for β-actin. Data shown are representative of three independent experiments.

DR3 / pNF-κB / NF-κB ; 

PubMed: 19670328     

Representative immunoblots showing expression of DR3, pNF-κB/p65 and NF-κB/p65 in cells treated with fisetin (0-40 μM).

p-mTOR / mTOR / Raptor / Rictor ; 

PubMed: 20530556     

Effect of fisetin on mTOR, Raptor and Rictor shown by western blot.

23861901 25016296 19670328 20530556
p-4EBP1 / p-eIF4B / p-rpS6 ; 

PubMed: 20530556     

Effect of fisetin on mTOR target proteins. Immunofluorescent imaging of mTOR target proteins upon fisetin treatment is shown. Cells were seeded and cultured for 24 h in two-chamber glass slides followed by treatment with indicated doses of fisetin for 48 h before fixing and labeling with antibodies. For labeling, anti-rabbit antibodies against (A) p-4EBP1 (S65), (B) p-eIF4B (S422) and (C) p-rpS6 (S235/S236) (all from Cell Signaling Technology) were used. Alexa Fluor goat and anti-rabbit IgGs (Invitrogen) were used as secondary antibodies. 4′,6-Diamidino-2-phenylindole (DAPI) was used to counter stain the nucleus; ×600.

uPA ; 

PubMed: 23940799     

Cells were fixed, permeabilized, and immunostained with anti-uPA antibody (red), and cell nuclei were counter-stained with DAPI reagent. 

20530556 23940799
Growth inhibition assay
Cell viability; 

PubMed: 19670328     

Cell growth and viability of human PaC AsPC-1cells treated with fisetin (0-80 μM for 24 and 48 h) as measured by MTT assay. Each concentration of fisetin was repeated in ten wells. The values are represented as percent viable cells where vehicle (DMSO)-treated cells were regarded as 100% viable. Data is represented as mean ± SE of three independent experiments. Inset: Structure of fisetin.



Solubility (25°C)

In vitro DMSO 57 mg/mL (199.13 mM)
Ethanol 3 mg/mL (10.48 mM)
Water Insoluble

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Chemical Information

Molecular Weight 286.24


CAS No. 528-48-3
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC(=C(C=C1C2=C(C(=O)C3=C(O2)C=C(C=C3)O)O)O)O

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID