Home Angiogenesis EGFR activator NSC228155 For research use only.

NSC228155

NSC228155 is an activator of EGFR. It binds to the sEGFR dimerization domain II and modulates EGFR tyrosine phosphorylation.

NSC228155 Chemical Structure

NSC228155 Chemical Structure

CAS: 113104-25-9

Selleck's NSC228155 has been cited by 6 publications

Purity & Quality Control

Batch: Purity: 99.65%
99.65

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Biological Activity

Description NSC228155 is an activator of EGFR. It binds to the sEGFR dimerization domain II and modulates EGFR tyrosine phosphorylation.
Targets
EGFR [2] KIX-KID interaction [3]
0.36 μM
In vitro
In vitro NSC 228155 promotes transactivation of several RTKs, including ErbB2 and ErbB3, Insulin R and IGF-1 R receptors in the cells. It stimulates dimerization of sEGFR domain II[1]. NSC 228155 can rapidly move across cell membranes and disperse within both cytoplasmic and nuclear compartments. It rapidly generates hydrogen peroxide within cells[2]. NSC 228155 is also a potent inhibitor of KIX-KID interaction(IC50 = 0.36 μM), but it is not particularly selective against CREB-mediated gene transcription in HEK 293T cells[3].
Cell Research Cell lines MDA MB468 cells
Concentrations 100 μM
Incubation Time 15 min
Method MDA MB468 cells serum-starved overnight are pre-incubated (or not) with 10 μM AG1478 or 2 μM PD 153035 for 90 min and then, where indicated, incubated with 100 μM NSC 228155 or CN 009543V or 150 ng/ml EGF or vehicle (0.2% DMSO) for 15 min. Proteins are blotted to nitrocellulose membrane and analyzed with biotinylated anti-pTyr P100, anti-pEGFR Y1068 and anti-EGFR (epitope in cytoplasmic region) antibodies.

Chemical Information & Solubility

Molecular Weight 290.25 Formula

C11H6N4O4S
CAS No. 113104-25-9 SDF Download NSC228155 SDF
Smiles C1=CC=[N+](C(=C1)SC2=CC=C(C3=NON=C23)[N+](=O)[O-])[O-]
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 10 mg/mL ( (34.45 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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