AG-490 (Tyrphostin B42)

Name: AG-490 (Tyrphostin B42)
Brand: Selleck Chemicals
SKU: S1143
Rating: 5 (99 reviews)

For research use only.

Catalog No.S1143 Synonyms: Zinc02557947

99 publications

AG-490 (Tyrphostin B42) Chemical Structure

CAS No. 133550-30-8

AG-490 (Tyrphostin B42, Zinc02557947) is an inhibitor of EGFR with IC50 of 0.1 μM in cell-free assays, 135-fold more selective for EGFR versus ErbB2, also inhibits JAK2 with no activity to Lck, Lyn, Btk, Syk and Src.

Selleck's AG-490 (Tyrphostin B42) has been cited by 99 publications

Oncol Rep, 2021, 10.3892/or.2021.7926

PubMed: 33432366 ( click the link to review the publication )

Acta Neuropathol, 2020, 10.1007/s00401-020-02226-7

PubMed: 33009951 ( click the link to review the publication )

Aging (Albany NY), 2020, 12(18):18084-18098

PubMed: 32957082 ( click the link to review the publication )

Carcinogenesis, 2020, 41(7):993-1004

PubMed: 31740922 ( click the link to review the publication )

Oncogenesis, 2020, 10;9(2):15

PubMed: 32041943 ( click the link to review the publication )

Front Pharmacol, 2020, 26;11:273

PubMed: 32273843 ( click the link to review the publication )

J Neurodev Disord, 2020, 12(1):29

PubMed: 33172406 ( click the link to review the publication )

Food Funct, 2020, 11(5):3941-3951

PubMed: 32338270 ( click the link to review the publication )

BMC Cancer, 2020, 20(1):632

PubMed: 32641093 ( click the link to review the publication )

Mol Cells, 2020, 43(8):749-762

PubMed: 32868491 ( click the link to review the publication )

Exp Biol Med (Maywood), 2020, 245(7):654-666

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J Appl Toxicol, 2020, 24

PubMed: 32212198 ( click the link to review the publication )

Connect Tissue Res, 2020, 5;1-16

PubMed: 32370570 ( click the link to review the publication )

J Interferon Cytokine Res, 2020, 40(2):116-124

PubMed: 31834821 ( click the link to review the publication )

Int J Med Sci, 2020, 17(18):3174-3189

PubMed: 33173437 ( click the link to review the publication )

Pharmazie, 2020, 75(6):255-260

PubMed: 32539921 ( click the link to review the publication )

Arch Biochem Biophys, 2020, 685:108330

PubMed: 32156533 ( click the link to review the publication )

Cell Prolif, 2020, 53(8):e12856

PubMed: 32648622 ( click the link to review the publication )

Mol Med Rep, 2020, 21(1):89-96

PubMed: 31746349 ( click the link to review the publication )

Virus Res, 2020, 281:197907

PubMed: 32113834 ( click the link to review the publication )

J Cancer, 2020, 11(16):4771-4782

PubMed: 32626524 ( click the link to review the publication )

Biochem Biophys Res Commun, 2020, 521(2):360-367

PubMed: 31668806 ( click the link to review the publication )

Oxid Med Cell Longev, 2020, 2020:9187406

PubMed: 32832009 ( click the link to review the publication )

J Surg Res, 2020, 248:98-108

PubMed: 31877436 ( click the link to review the publication )
Biochem Biophys Res Commun, 2020, 524(4):963-969

PubMed: 32059851 ( click the link to review the publication )

Anim Biotechnol, 2020, 1-13

PubMed: 32657254 ( click the link to review the publication )

Cancers (Basel), 2019, 11(12)E1939

PubMed: 31817171 ( click the link to review the publication )

Cancer Discov, 2019, 9(6):756-777

PubMed: 30862724 ( click the link to review the publication )

Aging (Albany NY), 2019, 11(17):6674-6690

PubMed: 31481647 ( click the link to review the publication )

J Cell Physiol, 2019, 234(9):15395-15406

PubMed: 30710340 ( click the link to review the publication )

J Cell Physiol, 2019, 234(11):21182-21192

PubMed: 31032948 ( click the link to review the publication )

Int J Biol Sci, 2019, 15(9):1882-1891

PubMed: 31523190 ( click the link to review the publication )

Cancer Cell Int, 2019, 19:57

PubMed: 30918473 ( click the link to review the publication )

Front Pharmacol, 2019, 10:620

PubMed: 31231218 ( click the link to review the publication )

Toxicol Appl Pharmacol, 2019, 362:59-66

PubMed: 30352208 ( click the link to review the publication )

Eur J Pharmacol, 2019, 854:289-297

PubMed: 31004602 ( click the link to review the publication )

Onco Targets Ther, 2019, 12:9069-9076

PubMed: 31802914 ( click the link to review the publication )

Oral Dis, 2019, 25(7):1769-1779

PubMed: 31365165 ( click the link to review the publication )

Can Respir J, 2019, 2019:2697376

PubMed: 31379980 ( click the link to review the publication )

Clin Cancer Res, 2018, 24(10):2417-2429

PubMed: 29463558 ( click the link to review the publication )

J Cell Physiol, 2018, 234(1):454-463

PubMed: 29932210 ( click the link to review the publication )

Mol Carcinog, 2018, 57(3):429-439

PubMed: 29215754 ( click the link to review the publication )

Biomed Pharmacother, 2018, 101:107-114

PubMed: 29477470 ( click the link to review the publication )

Int J Oncol, 2018, 52(5):1515-1527

PubMed: 29512698 ( click the link to review the publication )

Toxicology, 2018, 408:62-69

PubMed: 29981841 ( click the link to review the publication )

Am J Transl Res, 2018, 10(2):465-473

PubMed: 29511440 ( click the link to review the publication )

Eur J Pharmacol, 2018, 833:221-229

PubMed: 29890157 ( click the link to review the publication )

Oncol Rep, 2018, 39(4):1892-1900

PubMed: 29393444 ( click the link to review the publication )
Biomed Res Int, 2018, 2018:2548378

PubMed: 30363706 ( click the link to review the publication )

Neurosci Lett, 2018, 662:219-226

PubMed: 29061394 ( click the link to review the publication )

Oncol Lett, 2018, 16(2):2517-2524

PubMed: 30013646 ( click the link to review the publication )

Int Urol Nephrol, 2018, 50(8):1535-1544

PubMed: 29508174 ( click the link to review the publication )

Antioxid Redox Signal, 2017, 27(5):251-268

PubMed: 27923278 ( click the link to review the publication )

Cardiovasc Res, 2017, 113(3):310-320

PubMed: 28158495 ( click the link to review the publication )

Cell Physiol Biochem, 2017, 44(1):293-302

PubMed: 29132131 ( click the link to review the publication )

Int J Mol Sci, 2017, 18(12)

PubMed: 29206176 ( click the link to review the publication )

Am J Transl Res, 2017, 9(10):4617-4626

PubMed: 29118922 ( click the link to review the publication )

Brain Res, 2017, 15;1657:202-207

PubMed: 27998796 ( click the link to review the publication )

Drug Des Devel Ther, 2017, 11:2787-2799

PubMed: 29033541 ( click the link to review the publication )

Biosci Rep, 2017, 37(6)BSR20171038

PubMed: 29074558 ( click the link to review the publication )

PLoS One, 2017, 12(1):e0169665

PubMed: 28068414 ( click the link to review the publication )

Onco Targets Ther, 2017, 10:5329-5336

PubMed: 29184419 ( click the link to review the publication )

Cell Biol Int, 2017, 41(6):659-668

PubMed: 28378938 ( click the link to review the publication )

Evid Based Complement Alternat Med, 2017, 2017:2942830

PubMed: 28630633 ( click the link to review the publication )

Oncotarget, 2017, 8(57):96993-97008

PubMed: 29228588 ( click the link to review the publication )

Oncotarget, 2017, 8(57):96958-96969

PubMed: 29228585 ( click the link to review the publication )

Oncotarget, 2017, 8(39):64853-64866

PubMed: 29029396 ( click the link to review the publication )

Oncotarget, 2017, 8(28):45848-45861

PubMed: 28507278 ( click the link to review the publication )

Oncogene, 2016,

PubMed: 26804171 ( click the link to review the publication )

PLoS Pathog, 2016, 12(4):e1005605

PubMed: 27128969 ( click the link to review the publication )

Stem Cells Transl Med, 2016, 5(7):960-9

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Clin Exp Immunol, 2016, 184(2):147-58

PubMed: 26646950 ( click the link to review the publication )
Cellular Signalling, 2016, 19;28(6):652-662

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Inflamm Res, 2016, 65(3):193-202

PubMed: 26621504 ( click the link to review the publication )

Int J Mol Med, 2016, 38(4):1125-34

PubMed: 27599586 ( click the link to review the publication )

J Bone Miner Metab, 2016, [Epub ahead of print]

PubMed: 27628046 ( click the link to review the publication )

Scand J Immunol, 2016,

PubMed: 26482544 ( click the link to review the publication )

Mol Med Rep, 2016, 13(4):3684-90

PubMed: 26936086 ( click the link to review the publication )

Oncotarget, 2016, 7(27):41445-41459

PubMed: 27213590 ( click the link to review the publication )

J Am Soc Nephrol, 2016, 27(10):3105-3116

PubMed: 27694161 ( click the link to review the publication )

Cancer Lett, 2015, 359(2):335-43

PubMed: 25641338 ( click the link to review the publication )

Carcinogenesis, 2015, 36(1):142-50

PubMed: 25411359 ( click the link to review the publication )

Carcinogenesis, 2015, 36(1):142-50

PubMed: ( click the link to review the publication )

Neoplasia, 2015, 17(8):625-33

PubMed: 26408255 ( click the link to review the publication )

Eur Neuropsychopharmacol, 2015, 25(8):1287-99

PubMed: 25979764 ( click the link to review the publication )

Eur Neuropsychopharm, 2015, 10.1016/j.euroneuro.2015.04.020

PubMed: ( click the link to review the publication )

Int J Neuropsychopharmacol, 2015, 19(2)

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Oncol Rep, 2015, 34(4):1875-82

PubMed: 26238612 ( click the link to review the publication )

J Geriatr Cardiol, 2015, 12(6):591-9

PubMed: 26788034 ( click the link to review the publication )

Mol Cancer, 2014, 13:176

PubMed: 25047660 ( click the link to review the publication )

Carcinogenesis, 2014, 35(4):795-806

PubMed: 24265293 ( click the link to review the publication )

Mol Cell Biol, 2014, 34(24):4368-78

PubMed: 25312644 ( click the link to review the publication )

Am J Physiol Cell Physiol, 2014, 307(7):C597-605

PubMed: 24944200 ( click the link to review the publication )

Oncotarget, 2014, 5(16):7051-64

PubMed: 25216522 ( click the link to review the publication )

Clin Cancer Res, 2013, 19(17):4697-705

PubMed: 23857601 ( click the link to review the publication )

Cancer Lett, 2013, 341(2):224-30

PubMed: 23941832 ( click the link to review the publication )
Biochem Biophys Res Commun, 2013, 429(3-4):156-62

PubMed: 23142594 ( click the link to review the publication )

Biochem Biophys Res Commun, 2013, 435(4):533-9

PubMed: 23665018 ( click the link to review the publication )

Prostate, 2012, 73(3):267-77

PubMed: 22821817 ( click the link to review the publication )

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description

Targets

JAK2 (V617F) ^[8] ()	EGFR ^[1] (Cell-free assay)
	0.1 μM

In vitro

AG-490 inhibits HER-2 driven cell proliferation with IC50 of 3.5 μM. ^[1] Corresponding to the specific dose-dependent inhibition of constitutively activated JAK2 in pre-B acute leukemia (ALL) cells, AG-490 (5 μM) almost completely blocks the growth of all ALL cells by inducing programmed cell death, with no deleterious effect on normal hematopoiesis. AG-490 does not inhibit the activities of Lck, Lyn, Btk, Syk, and Src. ^[2] AG-490 (60-100 μM) blocks the constitutive activation of Stat3sm, and inhibits spontaneous as well as interleukin 2-induced growth of mycosis fungoides (MF) tumor cells with IC50 values of 75 μM and 20 μM, respectively. ^[3] AG-490 potently inhibits IL-2-mediated human T cell growth with an IC50 of 25 μM by blocking the activities of JAK3 and STAT5a/b. ^[4] Although AG-490 alone has no effect on proliferation of FDrv210H cells at a concentration of 5 μM, AG-490 can synergize with STI571 to enhance its inhibitory effect on p210^bcr-abl driven proliferation. ^[5] AG-490 significantly inhibits the constitutive activation of Stat3 in MOPC, MPC11, and S194 cells, leading to dramatic dose-dependent apoptosis. ^[6] AG-490 (100 μM) inhibits Akt phosphorylation, inhibits the activation of nuclear factor-κB, and causes the activation of GSK-3β, leading to the reduction of c-Myc. AG-490 (50 μM) can induce apoptosis of imatinib-resistant BaF3 cells expressing T315I and E255K mutants of Bcr-Abl. ^[7] AG-490 at 30 μM inhibits not only Epo-induced phosphorylation of wild-type JAK2 but also constitutive phosphorylation of the JAK2 V617F mutant. AG-490 also potently inhibits cytokine-independent cell growth induced by the JAK2 V617F mutant in BaF3 cells. ^[8]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
BC3	M2XvbmZ2dmO2aX;uJGF{e2G7	MX[xNFDjiIoEtV2=	NFO2RmIzPCCq		NF\BUXZu\WSrYYTld{BRTUxiY3XscEBieG:ydH;zbZM>	MY[yOlE5PDl7OR?=
BCBL1	MVfGeY5kfGmxbjDBd5NigQ>?	NWLCdVc{OTBy4pEJxtVO	NFGxelYzPCCq		M2TwWY1m\GmjdHXzJHBGVCClZXzsJIFxd3C2b4Ppdy=>	NF3UdIQzPjF6NEm5PS=>
BC3	MYLGeY5kfGmxbjDBd5NigQ>?	NIjESWEyODEkgJpCuW0>	NIfKeFUzPCCq		NVLVTVA6dWWmaXH0[ZMh\GVvcHjvd5Bpd3K7bHH0bY9vKG:oIGPURXQ{KGOxcoLlcIF1\WRid3n0bEBJW1B5MDDhcoQhUFOIMTDy[YR2[3Srb36=	M3r1UVI3OTh2OUm5
BCBL1	MW\GeY5kfGmxbjDBd5NigQ>?	M1rzRVExOOLCidM1US=>	Mln0NlQhcA>?		MUHt[YRq[XSnczDk[U1xcG:\|cHjvdplt[XSrb36gc4YhW1SDVEOgZ49zemWuYYTl[EB4cXSqIFjTVFcxKGGwZDDIV2YzKHKnZIXjeIlwdg>?	NY\wSYc4OjZzOES5PVk>
BC3	NY\x[4lQTnWwY4Tpc44hSXO\|YYm=	MlLINVAx6oDLwsXN	NUj3bII6OjRiaB?=		NV7hVnFicW6mdXPld{BiKGOxbYDs[ZRmKGG3dH;wbIFocWNiZnz1fOKh	Ml;MNlYyQDR7OUm=
BCBL1	MY\GeY5kfGmxbjDBd5NigQ>?	Ml3RNVAx6oDLwsXN	MU[yOEBp		NYjPU3RKcW6mdXPld{BiKGOxbYDs[ZRmKGG3dH;wbIFocWNiZnz1fOKh	M{HITVI3OTh2OUm5
SK-MEL-28	NVXDXXpLTnWwY4Tpc44hSXO\|YYm=	MXm1NE8yODEkgJpCuW0>	NEnxNVA1QCCq	MWXEUXNQ	MVry[YR2[2W\|IHHuc4lscXNicnXzbZN1[W6lZR?=	M2HPUVI2OjF4NUKy
MeWo	M3PrNGZ2dmO2aX;uJGF{e2G7	MlPzOVAwOTBy4pEJxtVO	NEXLdYg1QCCq	NXrDUpQyTE2VTx?=	NH;0TnNz\WS3Y3XzJIFvd2mtaYOgdoV{cXO2YX7j[S=>	MlrJNlUzOTZ3MkK=
SK-MEL-5	MoSzSpVv[3Srb36gRZN{[Xl?	Ml3rOVAwOTBy4pEJxtVO	NIfoZXc1QCCq	M4n4VWROW09?	NHizT2Nz\WS3Y3XzJIFvd2mtaYOgdoV{cXO2YX7j[S=>	M{PHfFI2OjF4NUKy
SK-MEL-2	NUDpcmprTnWwY4Tpc44hSXO\|YYm=	NFzIXHo2OC9zMEFihKnDvU1?	M3XtPVQ5KGh?	NHPyb2pFVVOR	NXTxepZqemWmdXPld{Bidm:ra3nzJJJme2m\|dHHuZ4U>	M4rRdVI2OjF4NUKy
B16-F0	MV3GeY5kfGmxbjDBd5NigQ>?	MXq1NE8yODEkgJpCuW0>	NFftboI1QCCq	NXjObHQ{TE2VTx?=	MUTy[YR2[2W\|IHHuc4lscXNicnXzbZN1[W6lZR?=	MXyyOVIyPjV{Mh?=
TRPM2/HEK	MVXGeY5kfGmxbjDBd5NigQ>?	M1rsZ\|AvOeLCk{K1xsDDvU1?	NUTYXnd2OTYEoH3pci=>	MY\EUXNQ	NFPjT2Zz\WS3Y3XzJGgzVzJvaX7keYNm\CCFYUKrbY5kemWjc3WgbY4h[SClb37j[Y51emG2aX;uMYRmeGWwZHXueEBu[W6wZYKsJIFv\CC2aHWgTWM2OMLidnHseYUhf2G\|IEGuO:KhyrWP	NHizdJQzPTF5OUW3OC=>
U937	M3zCW2Z2dmO2aX;uJGF{e2G7	MVWwMlHjiJN{NdMgxtVO	NWPncHJFOTYEoH3pci=>	NWLlV5ZjTE2VTx?=	NX;0OYg1emWmdXPld{BJOk9{LXnu[JVk\WRiQ3GyL4lv[3KnYYPlJIlvKGFiY3;uZ4VvfHKjdHnvck1l\XCnbnTlcpQhdWGwbnXyMEBidmRidHjlJGlEPTEEoI\hcJVmKHejczCwMlTDqML3TR?=	NUfGSohQOjVzN{m1O\|Q>
TRPM2/HEK	Mlm3SpVv[3Srb36gRZN{[Xl?	MYqxNOKhyrWP	NGSzfVY1OMLibXnu	MXnEUXNQ	Mmi4doVlfWOnczDUVnBOOiCjY4TpeoF1cW:wIHX2[Y4h[XRiaHnnbEBkd26lZX70doF1cW:wczDv[kBJOk9{	MoL5NlUyPzl3N{S=
GL37	MYfD[YxtKF[rYXLpcIl1gSCDc4PhfS=>	MlvNNE0yOMLiwsXN	MmfXOFghcA>?		NU\6ZlQ3e3WycILld5NmeyCOYTDlfJBz\XO\|aX;u	MYCyOFk6QTZ3Nx?=
NRK-52E	NGfsOZFHfW6ldHnvckBCe3OjeR?=	MkXONeKhyrWP	M3XoeVExKG2rbh?=		NGrDN3ZjdG:la4OgeIhmKHO2aX31cIF1d3K7IHXm[oVkfCCxZjDBcochUUlib36gVIF5NTJiZYjwdoV{e2mxbtMg	NFvqXIgzPDdzMESyNy=>
NRK-52E	NIHveY9HfW6ldHnvckBCe3OjeR?=	NVnBSph[OcLiwsXN	NGPhZXYyOCCvaX6=		MkfOZoxw[2u\|IFHu[{BKUSCrbnT1Z4VlKEOGMkSg[ZhxemW\|c3nvci=>	NX7GclNoOjR5MUC0NlM>
HSC	M4P4cGZ2dmO2aX;uJGF{e2G7	Mn3KNlAh\|ryP	MkfUNUBp		M1XKOIFjem:pYYTld{B1cGViZHnm[oVz\W62aXHsJIVn\mWldIOgc4YhdGWydHnuJI9zKEGJRYO=	NXnkPWx[OjR4MUSxPVk>
EJ	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MYS1NE85OCEQvF2=	M3fvdVI1NzR6L{eyJIg>		NF7p[25qdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDic5RpKHSrbXWgZY5lKGSxc3Wg[IVx\W6mZX70JI1idm6nch?=	NVXBeXM1OjR3OEewOFk>
EJ	NUjQdJR{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M1PHWFUxNzhyIN88US=>	M3P2Z\|Q5KGh?		M3P6UYNifXOnczDTMZBp[XOnIHHydoV{fA>?	MWCyOFU5PzB2OR?=
EJ	MoXYSpVv[3Srb36gRZN{[Xl?	NFzvdG42OC96MDFOwG0>	NHTkPWs1QCCq		NYPKVoUy\G:5boLl[5Vt[XSnczDjMW16[yxiY4njcIlvTDFuIIP1dpZqfmmwIHHu[EBXTUeIIHX4dJJme3Orb37z	M1nZb\|I1PTh5MES5
HepG2	M3rVTGZ2dmO2aX;uJGF{e2G7	M1fEeVUxNTVyMDFOwG0>	MoL0OlDDqG2rbh?=		MWXpcohq[mm2czD0bIUhUUxvNj3pcoR2[2WmIIDoc5NxcG:{eXzheIlwdiCxZjDTWGFVOSBqVInyO\|A2MSCjbnSgV3RCXDNiKGT5dlcxPSliaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ?	MXqyOFI1OjB2Nh?=
SGC7901	MXXD[YxtKF[rYXLpcIl1gSCDc4PhfS=>	M2XIS\|AuOTByIN88US=>	NITiV5MzPC92OD:3NkBp		MkDUZ4F2e2W\|IHGgd4lodmmoaXPhcpQhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGSxc3Wt[IVx\W6mZX70cJkh[nW2IH7veEB1cW2nLXTldIVv\GWwdHz5	M{j4ZVI1OTVzMkW1
AGS	NX[1NGVrS2WubDDWbYFjcWyrdImgRZN{[Xl?	MVqwMVExOCEQvF2=	MlfxNlQwPDhxN{KgbC=>		MmSxZ4F2e2W\|IHGgd4lodmmoaXPhcpQhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGSxc3Wt[IVx\W6mZX70cJkh[nW2IH7veEB1cW2nLXTldIVv\GWwdHz5	M{nXVVI1OTVzMkW1
SGC7901	NUTORmpzTnWwY4Tpc44hSXO\|YYm=	NHT3fHE2OCEQvF2=	M2HJ[FI1NzR6L{eyJIg>		Mo\YeIhmKGyndnXsd{Bw\iCySlHLNkBj\WejbjD0c{Bl\WOuaX7lJIF1KDJ2IHjyMEBidmRicnXic5Vv\GWmIHH0JFczKGi{wrC=	MXSyOFE2OTJ3NR?=
AGS	NXW2S4MzTnWwY4Tpc44hSXO\|YYm=	NWnVd5hNPTBizszN	NYjUOJppOjRxNEivO\|IhcA>?		NFzVc3d1cGVibHX2[Yx{KG:oIIDKRWszKGKnZ3HuJJRwKGSnY3zpcoUh[XRiMkSgbJItKGGwZDDy[YJwfW6mZXSgZZQhPzJiaINCpC=>	NFjTfmYzPDF3MUK1OS=>
SGC7901	M1u3e2Z2dmO2aX;uJGF{e2G7	MXW1NEDPxE1?	M2X0bFI1NzR6L{eyJIg>		MlvSeIhmKGO7dH;wcIF{dWmlIHzvZ4FtcXqjdHnvckBw\iCySlHLNkApUkGNMjDwbI9{eGixconsZZRm\CCjdDDy[ZNq\HWnczDUfZIyODB5IHHu[EBVgXJzMEC4LUBl\WO{ZXHz[YQh[W[2ZYKgRWc1QTBidILlZZRu\W62IH\vdkAzPCCjbnSgOFghcHJuIHL1eEB{fGG{dHXkJJRwKHKnYn;1coQh[XRiN{KgbJI>	NFjzVpIzPDF3MUK1OS=>
AGS	NV7IRWp2TnWwY4Tpc44hSXO\|YYm=	M{jM[FUxKM7:TR?=	NFnuXXozPC92OD:3NkBp		M3TqWJRp\SCleYTvdIxie22rYzDsc4NidGm8YYTpc44hd2ZicFrBT\|IhMEqDS{KgdIhwe3Cqb4L5cIF1\WRiYYSgdoV{cWS3ZYOgWJlzOTByNzDhcoQhXHm{MUCwPEkh\GWlcnXhd4VlKGGodHXyJGFIPDlyIITy[YF1dWWwdDDmc5IhOjRiYX7kJFQ5KGi{LDDieZQhe3SjcoTl[EB1dyC{ZXLveY5lKGG2IEeyJIhz	NGfK[pYzPDF3MUK1OS=>
MC3T3-E1	M{e2RmZ2dmO2aX;uJGF{e2G7	MlnEOVAh\|ryP	NHHWbpc1KGh?		M3XlXIlvcGmkaYTzJGhUTS2rbnT1Z4VlKEKPUEegZY5lKEeKUjDwdo91\WmwIHX4dJJme3Orb39CpC=>	MXiyN\|g4Pzd\|NB?=
7TD1-DXM	NHv2NJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3i1R\|ExKM7:TR?=	NEn6R\|Q4OiCq	MU\EUXNQ	NH3W[Y9qdmirYnn0d{Bk\WyuIHfyc5d1cA>?	MUKyN\|g4OTF3OR?=
7TD1-WD-90	NH3J[\|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NH;MXpEyOCEQvF2=	NXzKU3RUPzJiaB?=	NWPtXXlTTE2VTx?=	NHXMXnFqdmirYnn0d{Bk\WyuIHfyc5d1cA>?	MVOyN\|g4OTF3OR?=
7TD1-DXM	NVTqOIF{SXCxcITvd4l{KEG\|c3H5	NFLkUG02OCEQvF2=	MX20PEBp	NX;nWJh7TE2VTx?=	M2fRNolv\HWlZYOgZZBweHSxc3nz	NI\LTVczOzh5MUG1PS=>
7TD1-WD-90	Mm\ORZBweHSxc3nzJGF{e2G7	NGfGTHc2OCEQvF2=	M4jUT\|Q5KGh?	MoX5SG1UVw>?	MnWzbY5lfWOnczDhdI9xfG:\|aYO=	MVyyN\|g4OTF3OR?=
7TD1-WD-90	NFvTSlVHfW6ldHnvckBCe3OjeR?=	NUDpNpNqPTBizszN	NUHvW4dzPiCq	MUjEUXNQ	M2XMZZNq\26rZnnjZY51dHliaX7obYJqfHNidHjlJJBpd3OyaH;yfYxifGmxbjDv[kBLSUt{IHHu[EBxcG:\|cHjvdplt[XSrb36gc4YhW1SDVEO=	MmPxNlM5PzFzNUm=
HepG2	M{ftVGZ2dmO2aX;uJGF{e2G7	NYjifZRGOTByIN88US=>	MofvNVIwOjRiaB?=		MnrWbY5pcWKrdIOgV3RCXDNidInyc5NqdmVicHjvd5Bpd3K7bHH0bY9v	M{XkbVI{QDN4NECw
RAW264.7	Mnn3SpVv[3Srb36gRZN{[Xl?	NFLjdHc2OMLizszNxsA>	NFXQeoIzPC92ODDo		NUmzb4N[e3WycILld5NmeyCUQV7LUE1qdmS3Y3XkJI9{fGWxY3zhd5Rw\2WwZYPpdy=>	MkK4NlM3PjVyMUi=
RAW264.7	NX:3PIdyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MorzNE02OMLizszNxsA>	MkHVOFjDqGh?		MkPKbY5pcWKrdIOgZ4VtdCCpcn;3eIgh\G:\|ZT3k[ZBmdmSnboTsfS=>	M1zuOlI{PjZ3MEG4
RAW264.7	Mm\KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MXWwMVUxyqEQvF5CpC=>	M3LIRVQ5yqCq		M3\ININifXOnczDhckBienKnc4Sgc4YhWkGZMk[0Mlch[2WubIOgZZQhfGinIFewM2cyKHCqYYPlJI9nKHSqZTDj[YxtKGO7Y3zl	MmrlNlM3PjVyMUi=
RAW264.7	NWPJRXdrTnWwY4Tpc44hSXO\|YYm=	NH;zRpA2OMLizszN	MoDFNlQwPDhiaB?=		MnHnbY5pcWKrdIOgVmFPU0xvaX7keYNm\CCQRlHUZ\|Eh\XiycnXzd4lwdiCjbnSgdIhwe3Cqb4L5cIF1cW:wIH;mJHNmejd{N2PURXQ{	MUKyN\|Y3PTBzOB?=
A549	NHXtVWJHfW6ldHnvckBCe3OjeR?=	Mlm0NlAwPDBizszN	MXqyNEBp		M3nUc\|IxKM7:TTDBS\|Q6OCC\|dYDwdoV{e2W\|IITo[UBz[WSrYYTpc44ucW6mdXPl[EBqdn[jc3nvckBw\iCDNUS5JINmdGy\|	NVXVU3REOjN4MkCxPVE>
A549	NGXsTlNHfW6ldHnvckBCe3OjeR?=	MWCxNE8zOC92MDFOwG0>	MknwNlQhcA>?		NFvoeWd{fXCycnXzd4V{KHSqZTDyZYRq[XSrb36tbY5lfWOnZDDlcIV3[XSrb36gc4YhXkWJRtMg	MVKyN\|YzODF7MR?=
HUVECs	MlnpR4VtdCCYaXHibYxqfHliQYPzZZk>	NUP2VoF6OjBiwsXN	NFTRcGE1KGh?		NUHh[Zdj[XS2ZX71ZZRmeyCKMl:yMYlv\HWlZXSgZ4VtdCC\|aILpcoti\2ViYX7kJIlueHKxdnXkJJRp\SCjdIThZ4hu\W62IILheIUhd2ZidHjlJINmdGy\|	NHGySGgzOzR6M{m0Oi=>
HUVECs	MWLBdI9xfG:\|aYOgRZN{[Xl?	NFzJdWIzOCEEtV2=	M4jPdVQhcA>?		NF;C[Hh{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnczD0bIUh[2WubDDhdI9xfG:2aXOgbY5l\Xh?	MoDJNlM1QDN7NE[=
BV-2	NEPZb49HfW6ldHnvckBCe3OjeR?=	M{\TdVIxKML3TR?=	M{PWZVE3KGh?		MmWwbY5pcWKrdIOgUHBUNWmwZIXj[YQhW1SDVEGgdIhwe3Cqb4L5cIF1cW:wIIfpeIgh[Wyvb4P0JINwdXCuZYTlcJkh\GmvaX7pd4hm\CCrTl;TJIV5eHKnc4Ppc44>	NYD4eGRQOjN{M{[zO\|A>
NRK-52E	MnnWSpVv[3Srb36gRZN{[Xl?	NVj0S\|NqPcLizszN	MlrLN\|DDqG2rbh?=		NWCwSo1G[XS2ZX71ZZRmeyCDbnetLFHjiJN5KT3pcohq[mm2ZXSgWGdHNc7{MTDtVm5CKGG2IEG2xsBpyqB?	M3nlOlI{OTd2N{W3
SW620	NFfqbIdHfW6ldHnvckBCe3OjeR?=	M2TKUVIxKML3TR?=	NFvWT3IyNzZiaB?=		NFPPe3lqdmirYnn0d{BxNVOWQWSzJIFkfGm4YYTpc44>	NF;KNYEzOzFzME[yOS=>
RPE	NEXldXJHfW6ldHnvckBCe3OjeR?=	NUjRdWlIOzBiwsXNxsA>	MnnFN{Bp		NVLoSFdbcW6qaXLpeJMhfGinIHnu[JVkfGmxbjDv[kBxNVOWQWSzJIV5eHKnc4Ppc44>	NVvMXlhPOjNyOUSwOlc>
SW1116	Mn7nSpVv[3Srb36gRZN{[Xl?	NHHiPW0yODBiwsXNxsA>	NFLDeWgzPC92OD:3NkBp		NUTQPWt{\GWlcnXhd4V{KHSqZTDlfJBz\XO\|aX;uJI9nKEqDS{KgZY5lKHCMQVuyJJRqdWVvZHXw[Y5l\W62bIm=	MUiyNlA2ODd7MB?=
HT29	NW\xRWxNTnWwY4Tpc44hSXO\|YYm=	M2WyWVExOCEEtV5CpC=>	MnzCNlQwPDhxN{KgbC=>		M33aWYRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBLSUt{IHHu[EBxUkGNMjD0bY1mNWSncHXu[IVvfGy7	MWCyNlA2ODd7MB?=
SW1116	NX[yRnR2TnWwY4Tpc44hSXO\|YYm=	M{TLcFExOCEEtV5CpC=>	MUSyOE81QC95MjDo		M{\xe4Rm[3KnYYPld{B1cGVicGPURXQ{KGyndnXsd{BqdiCjIITpcYUu\GWyZX7k[Y51KG2jbn7lduKh	NXu4NYlNOjJyNUC3PVA>
HT29	MVTGeY5kfGmxbjDBd5NigQ>?	NUnnUWg6OTByINM1UeKh	MmKwNlQwPDhxN{KgbC=>		MXHk[YNz\WG\|ZYOgeIhmKHCVVFHUN{Bt\X[nbIOgbY4h[SC2aX3lMYRmeGWwZHXueEBu[W6wZYNCpC=>	NFzvdHUzOjB3MEe5NC=>
ARPE-19	NYjVPY5jTnWwY4Tpc44hSXO\|YYm=	MUG1xsDPxE1?	M3XRUlMxyqCvaX6=		MknmbY5pcWKrdIOgTmFMOiCyaH;zdIhwemmuYYTpc44>	NEnUZm4zOTZ{MEm2Ny=>
HSC-T6	Mn7mRZBweHSxc3nzJGF{e2G7	NIXMW3AyOMLizszN	NIK3S44zyqCqwrC=		NEmxRmNqdmirYnn0d{B1cGViYYDvdJRwe2m\|IH;mJGhUSy2WNjDj[YxteyCrbnT1Z4VlKGK7IFPESS=>	NGq3[Y4zOTN7Nkm5PC=>
HSC-T6	NEflSXZHfW6ldHnvckBCe3OjeR?=	NVnW[2I4OTEEoN88US=>	MWCyxsBpyqB?		NHXBVYRqdmirYnn0d{B1cGViZYjwdoV{e2mxboOgc4YheFlvU2TBWFEh[W6mIFLh[EBqdmS3Y3XkJIJ6KEOGRR?=	MY[yNVM6Pjl7OB?=
Hep-2	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MWqyOU0yODBizszN	NVS1S\|ZbOjRxNEivO\|IhcA>?		M2LXS4lvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHLveIghfGmvZTDhcoQh\G:\|ZTDk[ZBmdmSnboSgcYFvdmW{	MmC4NlE{ODl2OEG=
Hep-2	M37RS2Fxd3C2b4Ppd{BCe3OjeR?=	Mn;WOVAh\|ryP	MkHJNlQwPDhxN{KgbC=>		M1X6fIlv\HWlZYOgZ4VtdCCjcH;weI9{cXNidHnt[UBl\XCnbnTlcpRtgQ>?	Mo\MNlE{ODl2OEG=
Hep-2	M17TVWZ2dmO2aX;uJGF{e2G7	MljTOVAh\|ryP	MUSyOE81QC95MjDo		MVTpcohq[mm2czDHNUB1dyCVIHPlcIwh[3mlbHWgeJJidnOrdHnvckBidmRiaX7keYNmeyCJMTDj[YxtKGO7Y3zlJIFzemW\|dB?=	M4TTWFIyOzB7NEix
Hep-2	NGC4VXJHfW6ldHnvckBCe3OjeR?=	NGTlR2o2OCEQvF2=	MUKyOE81QC95MjDo		M{LjToRwf26{ZXf1cIF1\XNidHjlJHNVSVR\|LDDwMXNVSVR\|IHHu[EB{fXK4aY\pckBxem:2ZXnuJIxmfmWucx?=	MnHUNlE{ODl2OEG=
KF8	Ml\tSpVv[3Srb36gRZN{[Xl?	NV\YUlZbOTEEoN88UeKh	NW\qPFY3OcLiaB?=	NHvC[ZBFVVORwrC=	Mlz3bY5pcWKrdIOgTWwuOzNvaX7keYNm\CCQRj5OvmIh[WO2aY\heIlwdg>?	MUOyNFk1ODB2NR?=
KF8	MoPqSpVv[3Srb36gRZN{[Xl?	NEjYboUyOMLizszNxsA>	NFrZUG4yyqCqwrC=	NUe0SVVrTE2VT9Mg	M2DNdYlvcGmkaYTzJGlNNTN\|LXnu[JVk\WRiSd86Ru6yKGSnZ4Lh[IF1cW:wIHHu[EBPTi4QulKgZYN1cX[jdHnvci=>	M1L5dVIxQTRyMES1
HEL	M2fXWGZ2dmO2aX;uJGF{e2G7	MUSxNFDDqM7:TR?=	M13WblEzNTd{IHi=		MVTpcohq[mm2czD0bIUhdGW4ZXygc4YheC2MQVuyMEBLSUt{	M4PVZ\|IxPjJzME[x
HEL	NEHrR3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MojkNVAxyqEQvF2=	NHPKR\|YxNTViZB?=		NUjhSnQzemWmdXPld{Boem:5dHigc4YhUkGNMm[2NVdHNWW6cILld5NqdmdiSFXMJINmdGy\|	Mnv2NlA3OjFyNkG=
A-172	MmfiSpVv[3Srb36gRZN{[Xl?	M3;oSFUxNzFyMNMg{txO	M4exfVQ5KGh?		MY\y[YR2[2W\|IITo[UBt\X[nbIOgc4Yh[2:wc4TpeJV1cX[nbImgZYN1cX[jdHXkJHNVSVR\|IHnuJIEhfGmvZT3k[ZBmdmSnboSgZY5lKGSxc3Wt[IVx\W6mZX70JIZie2irb36=	NUPMZVBoOjB3OEm1NlU>
MZ-18	MWLGeY5kfGmxbjDBd5NigQ>?	NW\oOmt3PTBxMUCwxsDPxE1?	NEDG[W81QCCq		Mn;KdoVlfWOnczD0bIUhdGW4ZXzzJI9nKGOxboP0bZR2fGm4ZXz5JIFkfGm4YYTl[EBUXEGWMzDpckBiKHSrbXWt[IVx\W6mZX70JIFv\CCmb4PlMYRmeGWwZHXueEBn[XOqaX;u	MWiyNFU5QTV{NR?=
MZ-54	NUOwNW5ZTnWwY4Tpc44hSXO\|YYm=	NHTSNWw2OC9zMEFCpO69VQ>?	M1Tz[lQ5KGh?		MV3y[YR2[2W\|IITo[UBt\X[nbIOgc4Yh[2:wc4TpeJV1cX[nbImgZYN1cX[jdHXkJHNVSVR\|IHnuJIEhfGmvZT3k[ZBmdmSnboSgZY5lKGSxc3Wt[IVx\W6mZX70JIZie2irb36=	MUeyNFU5QTV{NR?=
MZ-256	NHGxTYRHfW6ldHnvckBCe3OjeR?=	NGH1dJY2OC9zMEFCpO69VQ>?	NUHoW25tPDhiaB?=		NF3PWWxz\WS3Y3XzJJRp\SCuZY\lcJMhd2ZiY3;ud5RqfHW2aY\lcJkh[WO2aY\heIVlKFOWQWSzJIlvKGFidHnt[U1l\XCnbnTlcpQh[W6mIHTvd4Uu\GWyZX7k[Y51KG[jc3jpc44>	MnPONlA2QDl3MkW=
MZ-304	M1qxdmZ2dmO2aX;uJGF{e2G7	Ml3rOVAwOTBywrFOwG0>	NI\pOFA1QCCq		M2SyWZJm\HWlZYOgeIhmKGyndnXsd{Bw\iClb37zeIl1fXSrdnXsfUBi[3SrdnH0[YQhW1SDVEOgbY4h[SC2aX3lMYRmeGWwZHXueEBidmRiZH;z[U1l\XCnbnTlcpQh\mG\|aHnvci=>	MmixNlA2QDl3MkW=
A-172	MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M2jBPFUxNzFyMNMg{txO	NWOwOlB6PDhiaB?=		NUjYNJZtdGWjZIOgeI8h[SC\|dHH0bZN1cWOjbHz5JJNq\26rZnnjZY51KHKnZIXjeIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36gc5ZmeiCjIITpcYUheGW{aX;kJI9nKDR6wrDo	MYCyNFU5QTV{NR?=
MZ-18	M2DXO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MWS1NE8yODEEoN88US=>	M1WyS\|Q5KGh?		MlH2cIVi\HNidH:gZUB{fGG2aYP0bYNidGy7IIPp[45q\mmlYX70JJJm\HWldHnvckBw\iClZXzsJJBzd2yrZnXyZZRqd25ib4\ldkBiKHSrbXWgdIVzcW:mIH;mJFQ5yqCq	NInhdYkzODV6OUWyOS=>
MZ-54	NXfuS\|NDT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MYO1NE8yODEEoN88US=>	NGfFO5c1QCCq		NYrXe2dndGWjZIOgeI8h[SC\|dHH0bZN1cWOjbHz5JJNq\26rZnnjZY51KHKnZIXjeIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36gc5ZmeiCjIITpcYUheGW{aX;kJI9nKDR6wrDo	NXnxdHJ[OjB3OEm1NlU>
MZ-256	M2exOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Mo\GOVAwOTBywrFOwG0>	NYqzVYlFPDhiaB?=		MWLs[YFleyC2bzDhJJN1[XSrc4TpZ4FtdHlic3nncolncWOjboSgdoVlfWO2aX;uJI9nKGOnbHygdJJwdGmoZYLheIlwdiCxdnXyJIEhfGmvZTDw[ZJqd2Rib3[gOFjDqGh?	MVmyNFU5QTV{NR?=
MZ-304	NEfMNJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M2fEV\|UxNzFyMNMg{txO	NEDuclU1QCCq		NIXufldt\WGmczD0c{BiKHO2YYTpd5Rq[2GubImgd4lodmmoaXPhcpQhemWmdXP0bY9vKG:oIHPlcIwheHKxbHnm[ZJifGmxbjDveoVzKGFidHnt[UBx\XKrb3Sgc4YhPDkEoHi=	NVrXPVVZOjB3OEm1NlU>
A-172	NIrWSGVHfW6ldHnvckBCe3OjeR?=	NVj1bo06PTBxMUCwxsDPxE1?	Mn;HOFghcA>?		MnLlbY5pcWKrdIOgcYloemG2aX;u	M{X6OVIxPTh7NUK1
MZ-18	NHGyclJHfW6ldHnvckBCe3OjeR?=	NI\pbVg2OC9zMEFCpO69VQ>?	MUe0PEBp		M2LKR4lvcGmkaYTzJI1q\3KjdHnvci=>	NG\VW4wzODV6OUWyOS=>
MZ-54	Mmf0SpVv[3Srb36gRZN{[Xl?	MmHZOVAwOTBywrFOwG0>	MYm0PEBp		MlLzbY5pcWKrdIOgcYloemG2aX;u	NVjVZplzOjB3OEm1NlU>
MZ-256	MlXQSpVv[3Srb36gRZN{[Xl?	NGXtTWo2OC9zMEFCpO69VQ>?	MX:0PEBp		MoTpbY5pcWKrdIOgcYloemG2aX;u	NV;hPXMyOjB3OEm1NlU>
MZ-304	NV7zR3JjTnWwY4Tpc44hSXO\|YYm=	MVO1NE8yODEEoN88US=>	NV;JcZc3PDhiaB?=		NEPYOIJqdmirYnn0d{BucWe{YYTpc44>	MnXuNlA2QDl3MkW=
A-172	MYXGeY5kfGmxbjDBd5NigQ>?	NFPXfVcyODEEoN88US=>	NIrtcZE1QCCq		NEm2d4JqdmirYnn0d{Bqdn[jc3nvci=>	NWW1V5JrOjB3OEm1NlU>
MZ-18	MXfGeY5kfGmxbjDBd5NigQ>?	MnP4NVAxyqEQvF2=	M37rNVQ5KGh?		MlzSbY5pcWKrdIOgbY53[XOrb36=	NWTxNpRkOjB3OEm1NlU>
MZ-54	MojqSpVv[3Srb36gRZN{[Xl?	NHvaR2wyODEEoN88US=>	MknQOFghcA>?		MWPpcohq[mm2czDpcpZie2mxbh?=	MWGyNFU5QTV{NR?=
MZ-256	NX3DcIsyTnWwY4Tpc44hSXO\|YYm=	M3HpTFExOMLizszN	MmnoOFghcA>?		M{PQN4lvcGmkaYTzJIlvfmG\|aX;u	M4LNVFIxPTh7NUK1
MZ-304	MonrSpVv[3Srb36gRZN{[Xl?	MXixNFDDqM7:TR?=	M1jVT\|Q5KGh?		Ml\mbY5pcWKrdIOgbY53[XOrb36=	M{TxVlIxPTh7NUK1
A-172	MkTSSpVv[3Srb36gRZN{[Xl?	NVznbZN1PTBxMUCwxsDPxE1?	NWW5bXA4PDhiaB?=		MkfEdoVlfWOnczD0doFve2O{aYD0bY9vKG:oIF3NVEBo\W6nczDhcoQhemWmdXPld{Bmdnq7bXH0bYMh[WO2aY\peJkhd2ZiTV3Qdy=>	M3fT[VIxPTh7NUK1
MZ-18	M3LBOGZ2dmO2aX;uJGF{e2G7	M3nl[FUxNzFyMNMg{txO	M4jCXVQ5KGh?		NHHpR2dz\WS3Y3XzJJRz[W6\|Y4LpdJRqd25ib3[gUW1RKGenbnXzJIFv\CC{ZXT1Z4V{KGWweontZZRq[yCjY4Tpeol1gSCxZjDNUXB{	Mnf3NlA2QDl3MkW=
MZ-54	M2C5fmZ2dmO2aX;uJGF{e2G7	Ml\BOVAwOTBywrFOwG0>	M3;xZlQ5KGh?		MVzy[YR2[2W\|IITyZY5{[3KrcITpc44hd2ZiTV3QJIdmdmW\|IHHu[EBz\WS3Y3XzJIVvgnmvYYTpZ{Bi[3Srdnn0fUBw\iCPTWDz	NHTCVIIzODV6OUWyOS=>
MZ-256	NF7tVllHfW6ldHnvckBCe3OjeR?=	NYTPO\|QyPTBxMUCwxsDPxE1?	NWHhSVBZPDhiaB?=		Mmi1doVlfWOnczD0doFve2O{aYD0bY9vKG:oIF3NVEBo\W6nczDhcoQhemWmdXPld{Bmdnq7bXH0bYMh[WO2aY\peJkhd2ZiTV3Qdy=>	NEn0[5QzODV6OUWyOS=>
MZ-304	NVPEcIt{TnWwY4Tpc44hSXO\|YYm=	MVK1NE8yODEEoN88US=>	MWO0PEBp		M1O3bZJm\HWlZYOgeJJidnOlcnnweIlwdiCxZjDNUXAh\2WwZYOgZY5lKHKnZIXj[ZMh\W68eX3heIlkKGGldHn2bZR6KG:oIF3NVJM>	MmmwNlA2QDl3MkW=
SW1990	MoPVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MlnuNlAh\|ryP	M4nvUlI1NzR6L{eyJIg>		NGTnVFNqdmirYnn0d{Bk\WyuIHfyc5d1cCC2aX3lJIRmeGWwZHXueIx6	M3rIbVIxPDh{OEW4
SW1990	MYLGeY5kfGmxbjDBd5NigQ>?	NE\HbXUzOCEQvF2=	M4\jWVI1KGh?		NV\HTFhU\GWlcnXhd4V{KHSqZTDlfJBz\XO\|aX;uJI9nKE2PUD2yJIFv\CCYRVfGJI1TVkG\|	M2K1OlIxPDh{OEW4
SW1990	MoXMSpVv[3Srb36gRZN{[Xl?	MUSyNEDPxE1?	NGPpOGMzPCCq		NYTHXWtE\GWlcnXhd4V{KHSqZTDpcpRmdnOrdImgc4YheC2VdHH0N{BmgHC{ZYPzbY9v	NEXJSYkzODR6Mki1PC=>
SW1990	NFjvOGNKdn[jc3nvckBCe3OjeR?=	NXftcJhvOjBizszN	M3T2c\|I1KGh?		M2HibpJm\HWlZYOgbY53[XOrb36gc4YhW1dzOUmwJINmdGy\|wrC=	NEDYVHczODR6Mki1PC=>
THP1	Mo\vSpVv[3Srb36gRZN{[Xl?	NHfy[5AyOCC3TR?=	MkDXN\|AhdWmwwrC=		NGLteIFqdmirYnn0d{BUXEGWMzD0fZJwe2mwZTDwbI9{eGixconsZZRqd25iYomgc5ZmeiB4MDW=	M3niOVIxOzl\|Nkmw
BMMC	NGD2O21HfW6ldHnvckBCe3OjeR?=	Mmj1NE0yOCEQvF2=	Ml7GNVXDqG2rbh?=		NEPldG1qdmirYnn0d{BNXEN2wrDy[Yxm[XOnIHnuJIEh\G:\|ZT3k[ZBmdmSnboSg[oF{cGmxbjD3bZRpKG6nYYKgZ49ueGyndHWgbY5pcWKrdHnvckBifCClb37j[Y51emG2aX;ud{Djsb5zMNMg{txO	MkGzNVk5OzV6NEW=
A549	MlW5SpVv[3Srb36gRZN{[Xl?	NWLzXlR3OTVizszt	M2jXUFEhcA>?		MXPpcohq[mm2czD0bIUheGixc4Doc5J6dGG2aX;uJI9nKFOWQWSxJI9vKHS7cn;zbY5mKDdyMTD3ZZMh\GW2ZXP0[YQhOTVibXnuJIFnfGW{IGPQSUBDKHS{ZXH0cYVvfA>?	NUTHd\|FGOTl6MEG2OlU>
OVCAR-3	M{fQPWZ2dmO2aX;uJGF{e2G7	MmTQNVAhfU1?	NGCwbmMyKGh?		MonFbY5pcWKrdIOgUHBCNWmwZIXj[YQhW1SDVEOgdIhwe3Cqb4L5cIF1cW:w	NFrsfW4yQTZ2N{O2Ny=>
PA-1	M{DS[WZ2dmO2aX;uJGF{e2G7	M{DtdVExKHWP	M4Sy[FEhcA>?		NYTNWm9scW6qaXLpeJMhVFCDLXnu[JVk\WRiU2TBWFMheGixc4Doc5J6dGG2aX;u	NXXh[lZpOTl4NEezOlM>
OVCAR-3	MlThSpVv[3Srb36gRZN{[Xl?	NHrJOmwyOCC3TR?=	NU\2d25yOSCq		MlntbY5pcWKrdIOgJGxRSS2rbnT1Z4VlKG:4YYLpZY4h[2GwY3XyJINmdGxibX;0bYxqfHl?	MmfoNVk3PDd\|NkO=
PA-1	NWP4WZJ[TnWwY4Tpc44hSXO\|YYm=	MmqxNVAhfU1?	NFfrUpgyKGh?		NFHvV\|dqdmirYnn0d{AhVFCDLXnu[JVk\WRib4\hdolidiClYX7j[ZIh[2WubDDtc5RqdGm2eR?=	MVmxPVY1PzN4Mx?=
Jurkat	NHrMOHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MV[1NEDPxE1?	Mly5NlQwPDhxN{KgbC=>		NILVOFNmdmijbnPld{BVWkGLTD3pcoR2[2W\|IHPlcIwh\3Kxd4ToJIlvcGmkaYTpc44>	NX3HfnN1OTl3NkS4PVE>
SUPT1	MnPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M13Bd\|UxKM7:TR?=	NUXP[o5UOjRxNEivO\|IhcA>?		MX3lcohidmOnczDUVmFKVC2rbnT1Z4V{KGOnbHyg[5Jwf3SqIHnubIljcXSrb36=	NGPrR3AyQTV4NEi5NS=>
Jurkat	M1izUmFxd3C2b4Ppd{BCe3OjeR?=	NVXVVGVLPTBizszN	MmTqNlQwPDhiaB?=		NFfrPHhmdmijbnPld{BVWkGLTD3pcoR2[2W\|IHPlcIwh[XCxcITvd4l{	NVnYZXU1OTl3NkS4PVE>
SUPT1	MkS2RZBweHSxc3nzJGF{e2G7	M1jjOFUxKM7:TR?=	MnHCNlQwPDhiaB?=		NEPr[HNmdmijbnPld{BVWkGLTD3pcoR2[2W\|IHPlcIwh[XCxcITvd4l{	MVyxPVU3PDh7MR?=

... Click to View More Cell Line Experimental Data

In vivo

Administration of AG-490 drastically reduces the numbers of CD45⁺ and HLA-DR⁺ cells from 48 % and 46% in bone marrow of untreated mice, as well as 38% and 22% in the spleen of untreated mice to undetectale levels. ^[2] In vivo administration of AG-490 causes murine myeloma tumor cell apoptosis but does not inhibit IL-12-mediated macrophage activation and IFN-γ production by lymphocytes. ^[6] Consistent with the in vitro blocking of JAK2 V617F mutant activity, AG-490 treatment at 0.5 mg/day for 10 days effectively inhibits JAK2 V617F mutant-induced tumorigenesis and tumor cell invasion in nude mice. ^[8] Combined therapy with AG-490 and IL-12 induces greater antitumor effects than either agent alone in a murine myeloma tumor model. ^[6]

Protocol

Kinase Assay: ^[1]	- Collapse In vitro kinase autophosphorylation: AG-490 is dissolved in DMSO 10%-H₂O-ethanol 45%. Crude membrane extracts (0.125 μg/mL) are preactivated with EGF (20 nM) in 50 mM HEPES buffer, pH 7.6, and 125 mM NaCl, for 15 minutes at 4 °C. Autophosphorylation activity of EGFR or ErbB2 kinase is assayed at 4 °C for 30 seconds in V-shaped 96-well plates. Membrane extracts (8 μL) are added to each well containing reaction mixture (12 μL, 50mM, HEPES, pH 7.4,125 mM NaCl, 12 mM M₈Ac₂, 2 mM MnCl₂, 1 mM NaVO₃, 1 μM ATP, and 1 μCi[γ-³²P]ATP, final concentrations) and increasing concentrations of AG-490 (4 μL). After termination by addition of hot sample buffer, the samples are run on a 6% SDS-polyacrylamide gel electrophoresis minigel, the gels dried, and autoradiography performed during the linear exposure time period. The receptor bands are scanned densitrometrically, and the results analyzed by the Ez-Fit program. For the analysis of autophosphorylation of JAK2, JAK2 is immunoprecipitated by using anti-JAK2 antibody from lysates of G2 cells pretreated for 16 hours with increasing concentrations of AG-490 (0-50 μM). Immune complexes are then immunoblotted with anti-phosphotyrosine antibody. A dose-dependent inhibition of in vitro kinase activity is demonstrated by assessing JAK2 autophosphorylation.
Cell Research: ^[2]	- Collapse Cell lines: Pre-B ALL Concentrations: Dissolved in DMSO, final concentrations ~ 50 μM Incubation Time: 16 hours Method: Cells are exposed to different concentrations of AG-490 for 16 hours. For the determination of cell proliferation, [³H]tymidine (1 μCi) is added 6 hours or more before the cultures are terminated. Cells are then collected and samples counted in a liquid scintillation counter. (Only for Reference)
Animal Research: ^[2]	- Collapse Animal Models: SCID mice intravenously injected with ALL cells Dosages: 0.85 mg + 0.5 mg daily Administration: Continuous pump infusion supplemented with daily intraperitoneal injections (Only for Reference)

References

- Collapse

Solubility (25°C)

In vitro	DMSO	58 mg/mL (197.07 mM)
	Water	Insoluble
	Ethanol	'6 mg/mL
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 5% DMSO+40% PEG300+5% Tween80+50% ddH2O For best results, use promptly after mixing.	6.25 mg/ml

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download AG-490 (Tyrphostin B42) SDF

Molecular Weight	294.30
Formula	C₁₇H₁₄N₂O₃
CAS No.	133550-30-8
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	Zinc02557947
Smiles	C1=CC=C(C=C1)CNC(=O)C(=CC2=CC(=C(C=C2)O)O)C#N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

% DMSO+ % + % Tween 80+ % ddH₂O

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Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
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Computed Result

C1=C0/X	C1:	LOG(C1):
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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
I would like to know whether AG490 (S1143) goes to CNS through BBB, or not?
Answer:
AG-490 can go through the BBB. You can see this reference: http://bloodjournal.hematologylibrary.org/content/111/4/2062.full.html.

EGFR Signaling Pathway Map

EGFR Inhibitors with Unique Features

Selective EGFR Inhibitor

Erlotinib HCl (OSI-744) : EGFR-selective, IC50=2 nM.
Most Potent EGFR Inhibitor

Afatinib (BIBW2992) : EGFR(wt), IC50=0.5 nM; EGFR(L858R), IC50=0.4 nM; EGFR(L858R/T790M), IC50=10 nM; HER2, IC50=14 nM.
FDA-approved EGFR Inhibitor

Lapatinib : Approved by FDA for breast cancer.
Newest EGFR Inhibitor

CO-1686 (AVL-301) ^New : Irreversible, mutant-selective EGFR inhibitor with K_i of 21.5 nM and 303.3 nM for EGFRL858R/T790M and EGFRWT, respectively.

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Erlotinib ^New

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Afatinib (BIBW2992) Dimaleate ^New

Afatinib (BIBW2992) Dimaleate irreversibly inhibits EGFR/HER2 including EGFR(wt), EGFR(L858R), EGFR(L858R/T790M) and HER2 with IC50 of 0.5 nM, 0.4 nM, 10 nM and 14 nM, respectively; 100-fold more active against Gefitinib-resistant L858R-T790M EGFR mutant. Afatinib (BIBW2992) Dimaleate induces autophagy.
Poziotinib (HM781-36B) ^New

Poziotinib (HM781-36B, NOV120101) is an irreversible pan-HER inhibitor with IC50 of 3.2 nM, 5.3 nM and 23.5 nM for HER1, HER2, and HER4, respectively. Poziotinib also induces apoptosis and G1 cell cycle arrest. Phase 2.
Gefitinib (ZD1839)

Gefitinib (ZD-1839, Iressa) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively. Gefitinib promotes autophagy and apoptosis of lung cancer cells via blockade of the PI3K/AKT/mTOR pathway.

Features:A potent EGFR tyrosine kinase inhibitor.
Erlotinib HCl (OSI-744)

Erlotinib HCl (OSI-744, CP358774, NSC 718781) is an EGFR inhibitor with IC50 of 2 nM in cell-free assays, >1000-fold more sensitive for EGFR than human c-Src or v-Abl.
Afatinib (BIBW2992)

Afatinib (BIBW2992) inhibits EGFR/ErbB irreversibly in vitro with IC50 of 0.5, 0.4, 10, 14, 1 nM for EGFR^wt, EGFR^L858R, EGFR^L858R/T790M ErbB2 (HER2) and ErbB4 (HER4), respectively. Afatinib induces autophagy.
Osimertinib (AZD9291)

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Lapatinib

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Rociletinib (CO-1686)

Rociletinib (CO-1686, AVL-301) is an irreversible, mutant-selective EGFR inhibitor with K_i of 21.5 nM and 303.3 nM for EGFR^L858R/T790M and EGFR^WT in cell-free assays, respectively. Phase 2.
AG-1478 (Tyrphostin AG-1478)

AG-1478 (Tyrphostin AG-1478, NSC 693255) is a selective EGFR inhibitor with IC50 of 3 nM in cell-free assays, almost no activity on HER2-Neu, PDGFR, Trk, Bcr-Abl and InsR. AG-1478 (Tyrphostin AG-1478) inhibits encephalomyocarditis virus (EMCV) and hepatitis c virus (HCV) by targeting phosphatidylinositol 4-kinase IIIα (PI4KA).

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AG-490 (Tyrphostin B42)