NSC228155

Catalog No.S8312 Batch:S831202

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Technical Data

Formula

C11H6N4O4S
Molecular Weight 290.25 CAS No. 113104-25-9
Solubility (25°C)* In vitro DMSO 15 mg/mL (51.67 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description NSC228155 is an activator of EGFR. It binds to the sEGFR dimerization domain II and modulates EGFR tyrosine phosphorylation.
Targets
EGFR [2] KIX-KID interaction [3]
0.36 μM
In vitro NSC 228155 promotes transactivation of several RTKs, including ErbB2 and ErbB3, Insulin R and IGF-1 R receptors in the cells. It stimulates dimerization of sEGFR domain II[1]. NSC 228155 can rapidly move across cell membranes and disperse within both cytoplasmic and nuclear compartments. It rapidly generates hydrogen peroxide within cells[2]. NSC 228155 is also a potent inhibitor of KIX-KID interaction(IC50 = 0.36 μM), but it is not particularly selective against CREB-mediated gene transcription in HEK 293T cells[3].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    MDA MB468 cells

  • Concentrations

    100 μM

  • Incubation Time

    15 min

  • Method

    MDA MB468 cells serum-starved overnight are pre-incubated (or not) with 10 μM AG1478 or 2 μM PD 153035 for 90 min and then, where indicated, incubated with 100 μM NSC 228155 or CN 009543V or 150 ng/ml EGF or vehicle (0.2% DMSO) for 15 min. Proteins are blotted to nitrocellulose membrane and analyzed with biotinylated anti-pTyr P100, anti-pEGFR Y1068 and anti-EGFR (epitope in cytoplasmic region) antibodies.

Selleck's NSC228155 has been cited by 6 publications

Fibulin7 Mediated Pathological Cardiac Remodeling through EGFR Binding and EGFR-Dependent FAK/AKT Signaling Activation [ Adv Sci (Weinh), 2023, 10(24):e2207631] PubMed: 37344348
ADAM12 promotes clear cell renal cell carcinoma progression and triggers EMT via EGFR/ERK signaling pathway [ J Transl Med, 2023, 21(1):56] PubMed: 36717944
Integrating Network Pharmacology and Experimental Validation to Elucidate the Mechanism of Yiqi Yangyin Decoction in Suppressing Non-Small-Cell Lung Cancer [ Biomed Res Int, 2023, 2023:4967544] PubMed: 36874921
Maintaining homeostasis of mitochondria and endoplasmic reticulum with NSC228155 alleviates cisplatin-induced acute kidney injury [ Free Radic Biol Med, 2022, S0891-5849(22)00053-3] PubMed: 35134531
Upregulation of LINC01426 promotes the progression and stemness in lung adenocarcinoma by enhancing the level of SHH protein to activate the hedgehog pathway [ Cell Death Dis, 2021, 12(2):173] PubMed: 33568633
Ellagic acid inhibits cell proliferation, migration, and invasion in melanoma via EGFR pathway [ Am J Transl Res, 2020, 12(5):2295-2304] PubMed: 32509220

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.