Genistein (NPI 031L)

Name: Genistein (NPI 031L)
Brand: Selleck Chemicals
SKU: S1342
Rating: 5 (20 reviews)

For research use only.

Catalog No.S1342

20 publications

CAS No. 446-72-0

Genistein (NPI 031L), a phytoestrogen found in soy products, is a highly specific inhibitor of protein tyrosine kinase (PTK) which blocks the mitogenic effect mediated by EGF on NIH-3T3 cells with IC50 of 12μM or by insulin with IC50 of 19 μM.

Selleck's Genistein (NPI 031L) has been cited by 20 publications

Nat Chem Biol, 2021, 10.1038/s41589-021-00772-z

PubMed: 33723432 ( click the link to review the publication )

EMBO J, 2021, e107403

PubMed: 34223653 ( click the link to review the publication )

Theranostics, 2021, 11(5):2364-2380

PubMed: 33500730 ( click the link to review the publication )

Elife, 2021, 10e65962

PubMed: 33890572 ( click the link to review the publication )

Acta Biomater, 2021, S1742-7061(21)00586-9

PubMed: 34492369 ( click the link to review the publication )

Hum Cell, 2021, 10.1007/s13577-021-00579-z

PubMed: 34304386 ( click the link to review the publication )

Theranostics, 2021, 11(5):2364-2380

PubMed: 33500730 ( click the link to review the publication )

Cancer Cell Int, 2020, 19;20:58

PubMed: 32099531 ( click the link to review the publication )

Hum Cell, 2020, 10.1007/s13577-020-00420-z

PubMed: 32870449 ( click the link to review the publication )

Hum Cell, 2020, 10.1007/s13577-020-00425-8

PubMed: 32886306 ( click the link to review the publication )

Int J Mol Sci, 2020, 21(14):5025

PubMed: 32708687 ( click the link to review the publication )

Front Aging Neurosci, 2020, 12:153

PubMed: 32625078 ( click the link to review the publication )

Mol Cell, 2019, 75(6):1103-1116.e9

PubMed: 31420216 ( click the link to review the publication )

Sci Rep, 2018, 8(1):10204

PubMed: 29977044 ( click the link to review the publication )

Cancer Manag Res, 2018, 10:6205-6216

PubMed: 30538567 ( click the link to review the publication )

Chem Res Toxicol, 2018, 31(2):127-136

PubMed: 29156121 ( click the link to review the publication )

Biosci Rep, 2018, 38(4)

PubMed: 29884767 ( click the link to review the publication )

Cell Physiol Biochem, 2018, 51(6):2564-2574

PubMed: 30562733 ( click the link to review the publication )

Drug Deliv, 2018, 25(1):1258-1265

PubMed: 29847210 ( click the link to review the publication )

J Electroanal Chem, 2015, 742:54–61

PubMed: / ( click the link to review the publication )

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description

Targets

EGFR ^[1]

topo II ^[1]

In vitro

Genistein is an ATP competitive inhibitor. Genistein inhibits tyrosine phosphorylation in isolated enzyme and receptor preparations and in whole cells including platelets, lymphocytes and a variety of cultured cells. It also inhibits EGF-stimulated phosphorylation in cultured cells as well as inhibition of Topo II (topoisomerase II). Genistein inhibits EGF-stimulated tyrosine phosphorylation in cultured A431 epidermoid carcinoma cells. Inhibition is competitive with ATP and noncompetitive with substrate. ^[1] Genistein blocks the mitogenic effect mediated by EGF, insulin and thrombin on NIH-3T3 cells.^[2] Genistein also acts as an agonist at the GPR30 receptor and binds to PPARγ and estrogen receptors. Genistein also binds to PPARγ, acting as an agonist at this receptor with K_i of 5.7 μM. ^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
BxPC3	NHLJe3pRem:uaX\ldoF1cW:wIHHzd4F6			M1HwWWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQojQR\|Mh[2WubDDsbY5mKGK7IF3UWEBie3OjeTygTWM2OD1\|MDFOwG0>	NF3OPYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNke4PVc{Pyd-MU[3PFk4Ozd:L3G+
BT20	NXjZbFMzWHKxbHnm[ZJifGmxbjDhd5NigQ>?			MXfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEKWMkCgZ4VtdCCuaX7lJIJ6KE2WVDDhd5NigSxiSVO1NF01PiEQvF2=	NIHCNVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNke4PVc{Pyd-MU[3PFk4Ozd:L3G+
ANN-1	M{S0O2N6fG:2b4jpZ{Bie3OjeR?=			NEXHS4ZEgXSxdH;4bYMh\W[oZXP0JI9vKHZvYXLsJJRz[W6\|Zn;ycYVlKG23cnnu[UBCVk5vMTDj[YxteyxiSVO1NF05\|ryP	Ml;3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDJyMU[wN{c,QDJyMU[wN\|ww[T5?
breast carcinoma cells	NWjzVZpnS3m2b4TvfIlkKGG\|c3H5			MUfDfZRwfG:6aXOg[YZn\WO2IH;uJG1ETi15IHj1cYFvKGK{ZXHzeEBk[XKlaX7vcYEh[2WubIOsJGlEPTB;MUWuNe69VQ>?	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85OjBzNkCzK\|45OjBzNkCzQE9iRg>?
3T3	NVTHXmxTS3m2b4TvfIlkKGG\|c3H5			NXTre21nS3m2b4TvfIlkKGWoZnXjeEBwdiB\|VEOgZ4VtdHNuIFnDOVA:OjUQvF2=	M2XxWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzh{MEG2NFMoRjh{MEG2NFM9N2F-
colon cells	MlLnR5l1d3SxeHnjJIF{e2G7			MYnDfZRwfG:6aXOg[YZn\WO2IH;uJHdqTHJiaIXtZY4h[2:ub36gZ4VtdHNuIFnDOVA:OjdwN988US=>	M3;OR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzh{MEG2NFMoRjh{MEG2NFM9N2F-
Hepa1clc7	NFnzb4ZEgXSxdH;4bYNqfHliYYPzZZk>		M1S0TlczKGi{cx?=	NYrnbVBMS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhUGWyYUHjcIM4KGOnbHzzJIFnfGW{IEeyJIhzeyxiSVO1NF0yOc7:TR?=	MkTNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByN{W3OFIoRjFyMEe1O\|QzRC:jPh?=
MCF7	M3fX[2Z2dmO2aX;uJIF{e2G7		MVm2JIhzew>?	MoLyTY5pcWKrdHnvckBw\iCyaH;yZo9tKGW\|dHXyMYlv\HWlZXSgc5JvcXSqaX7lJIRm[2G{Yn;4fYxie2ViaX6gbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB4IHjyd{whUUN3ME2yOu69VQ>?	M3njblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEe1O\|QzLz5zMEC3OVc1OjxxYU6=
3T3/A31	M{HIUGN6fG:2b4jpZ4l1gSCjc4PhfS=>	MWixJJRwKDFyMDD1[{9uVA>?	NILIbmw4OiCqcoO=	NH70PY1EgXSxdH;4bYNqfHliYXfhbY5{fCCEQVzCM4MhdW:3c3WgZ4xwdmWmIEPUN{9COzFiY3XscJMh[XRiMTD0c{AyODBidXevcWwh[W[2ZYKgO\|IhcHK\|IHL5JI5q\3Kxc3nuJIF{e2G7	Mn3iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByOU[4OlMoRjFyMEm2PFY{RC:jPh?=
SH-SY5Y	MkfiSpVv[3Srb36gZZN{[Xl?			NIH3dodC\2:waYP0JIFkfGm4aYT5JIlvKHS{YX7zZ5JqeHSrb37hcEBi[3SrdnH0bY9vKGG\|c3H5JIlvKFOKLWPZOXkhdmW3cn;icIF{fG:vYTDj[YxteyCneIDy[ZN{cW6pIFXzeJJw\2WwIILlZ4VxfG:{IHLleIEtKEWFNUC9NE4xODF5zszN	Ml3zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF7ME[yPFAoRjFzOUC2NlgxRC:jPh?=
HeLa	NHPWUZBHfW6ldHnvckBie3OjeR?=			Mk\JRYN1cX[jdHnvckBw\iCnc4Tyc4dmdiC{ZYPwc45{\SCnbHXt[Y51KGmwIFjlUIEh[2WubIOgd5Ri[my7IITyZY5{\mWldHXkJJdqfGhiaIXtZY4hTXO2cn;n[Y4hemWlZYD0c5Ih[mW2YT6sJGVEPTB;MD6wNFQy\|ryP	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTlyNkK4NEc,OTF7ME[yPFA9N2F-
HeLa	M{O1U2Z2dmO2aX;uJIF{e2G7			M4mzUGFkfGm4YYTpc44hd2ZiZYP0do9o\W5icnXzdI9ve2ViZXzlcYVvfCCrbjDI[WxiKGOnbHzzJJN1[WKueTD0doFve2[nY4Tl[EB4cXSqIHj1cYFvKEW\|dILv[4VvKHKnY3XweI9zKGGucHjhMkwhTUN3ME2wMlA1QM7:TR?=	NV;CR4k{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG5NFYzQDBpPkGxPVA3OjhyPD;hQi=>
Ishikawa	M3qzeGV{fHKxZ3XubYMh[XO\|YYm=		Mlr5OEBl[Xm\|	NELLeWtGe3S{b3flcolkKGGldHn2bZR6KGmwIHj1cYFvKEm\|aHnrZZdiKGOnbHzzJIF{e2W\|c3XkJIF{KGmwZIXjeIlwdiCxZjDhcItidGmwZTDwbI9{eGijdHHz[UBi[3Srdnn0fUBi\nSncjC0JIRigXNiYomgdIFz[S2waYTyc5Bp\W6xbDDy[Yxm[XOnIHHzd4F6NCCLQ{WwQVAvPTIQvF2=	M2PkflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{NUCyN\|A4Lz5zMkWwNlMxPzxxYU6=
Hepa-1c1c7	MoO0SpVv[3Srb36gZZN{[Xl?			MW\JcoR2[3Srb36gc4YhVkGGUFi6dZVqdm:wZTDy[YR2[3Sjc3WgbY4hdW:3c3WgTIVx[S1zY{HjO{Bk\WyuczDhd5Nme3OnZDDhd{BlenWpIHzleoVtKHKncYXpdoVlKHSxIHTveYJt\SCnbor5cYUh[WO2aY\peJkh[nliTWTUJIF{e2G7LDDDSF0zOi57zszN	NYj3WIRORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS1NVA2QTBpPkG0OVExPTlyPD;hQi=>
Hepa-1c1c7	MWTGeY5kfGmxbjDhd5NigQ>?			MoDhTY5pcWKrdHnvckBw\iCvb4Xz[UBJ\XCjLUHjNYM4KGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NF01PS5{zszN	NWPxW\|M{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS1NVA2QTBpPkG0OVExPTlyPD;hQi=>
T47D	Ml7LSZN1em:pZX7pZ{Bie3OjeR?=			NX;QcplDTXO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCWNEfEJINmdGy\|IHHzd4V{e2WmIHHzJIRzfWdibHX2[Ywh[2G3c3nu[{B{fGmvdXzheIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36g[ZF2cX[jbHXueEB1dyBzMDDwUUBme3S{YXTpc4wh[nliYXzhcYFzKGKudXWgZZN{[XluIFHjeIl3cXS7PUCuNFAy\|ryP	NGWweVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe4O\|Q{Pid-MUW3PFc1OzZ:L3G+
MCF7	M2rwT2V{fHKxZ3XubYMh[XO\|YYm=			M1\6SmV{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegd5RqdXWuYYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:wIHXxeYl3[WynboSgeI8hOTBicF2g[ZN1emGmaX;sJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gT1yLkCwN:69VQ>?	MlrOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5OEe0N\|YoRjF3N{i3OFM3RC:jPh?=
T47D	MXfFd5Rzd2enbnnjJIF{e2G7			NXHi[5EzTXO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCWNEfEJINmdGy\|IHHzd4V{e2WmIHHzJIRzfWdibHX2[Ywh[2G3c3nu[{B{fGmvdXzheIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36g[ZF2cX[jbHXueEB1dyBzMECgdG0h\XO2cnHkbY9tKGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDBZ5Rqfmm2eU2wMlAxQc7:TR?=	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd6N{SzOkc,OTV5OEe0N\|Y9N2F-
MCF7	MlrkSZN1em:pZX7pZ{Bie3OjeR?=			MV3Fd5Rzd2enbnnjJIFkfGm4aYT5JIlvKGi3bXHuJG1ETjdiY3XscJMh[XO\|ZYPz[YQh[XNiZIL1[{Bt\X[nbDDjZZV{cW6pIIP0bY12dGG2aX;uJI9nKGOnbHygdJJwdGmoZYLheIlwdiCncYXpeoFt\W62IITvJFExOCCyTTDld5Rz[WSrb3ygZpkh[WyjbXHyJIJtfWViYYPzZZktKEGldHn2bZR6RTBwMEGz{txO	M{X0WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{i3OFM3Lz5zNUe4O\|Q{PjxxYU6=
293T	NVrJS2tzTnWwY4Tpc44h[XO\|YYm=			MlHwSIl{eGyjY3Xt[Y51KG:oIGuzTH0yP2KndHGt[ZN1emGmaX;sJIZzd21icnXjc41jcW6jboSgbJVu[W5iRWLi[ZRiKGW6cILld5Nm\CCrbjCyPVNVKGOnbHzzMEBKSzVyPUCuNFAyO87:TR?=	NXHxPXN[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[yNVk1PjNpPkG2NlE6PDZ\|PD;hQi=>
293T	NYXqVY92TnWwY4Tpc44h[XO\|YYm=			M2T0OGRqe3CuYXPlcYVvfCCxZjDbN2heOTekZYThMYV{fHKjZHnvcEBnem:vIILlZ49u[mmwYX70JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJFI6O1RiY3XscJMtKEmFNUC9NE4xOjl2zszN	NH7oVWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkKxPVQ3Oyd-MU[yNVk1PjN:L3G+
Vero	NUPkdoNRS3m2b4TvfIlkcXS7IHHzd4F6			NW\UUII6S3m2b4TvfIlkcXS7IHHnZYlve3RiYX\ybYNidiCpcnXlckBud26tZYmgWoVzdyClZXzsd{whUUN3ME2zNk46\|ryP	MlO1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2NEGwOlYoRjF4NESxNFY3RC:jPh?=
U937	MmT5RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NWqzWZRbPzJiaILz	NGT6R\|JCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGW5N\|ch[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IGfTWE05KGG\|c3H5MEBKSzVyPUS4{txO	NXn1VI1vRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexOVgxPTRpPkG3NVU5ODV2PD;hQi=>
RAW 264	NW\OPHZTTnWwY4Tpc44h[XO\|YYm=		M1HGTlI1KGi{cx?=	NXHhNHdSUW6qaXLpeIlwdiCxZjCxJJVoN22uIFzQV{1{fGmvdXzheIVlKFSQRnHsdIhiKGGlY4XteYxifGmxbjDpckBTSVdiMk[0JINmdGy\|IHHmeIVzKDJ2IHjyd{whUUN3ME2xPE4y\|ryP	M{TsRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{KwNlQ3Lz5zN{OyNFI1PjxxYU6=
K562	NULseIpjT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NULE[4ZDPSCmYYnz	M2XncGdzd3e2aDDpcohq[mm2aX;uJI9nKEt3NkKgZ4VtdHNiYomgXHRVKGG\|c3H5JIFnfGW{IEWg[IF6eyxiSVO1NF0yPy53Nt88US=>	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRzMUC5Nkc,OTd2MUGwPVI9N2F-
HUVE12	NFq3O\|BHfW6ldHnvckBie3OjeR?=			NHnxcZpT\X[ncoPhcEBw\iCqeXTyc4dmdiCyZYLvfIll\S2rbnT1Z4VlKEyGSDDhZ5Rqfmm2eTDpckBpfW2jbjDIWXZGOTJiY3XscJM>	Mk\NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyNki5PFAoRjF6ME[4PVgxRC:jPh?=
HL60	M4TPV2FvfGmuZYXr[Y1q[yCjc4PhfS=>		NGjYWHEzPCCqcoO=	NIfTd3lCdnSrbHX1b4VucWNiYXP0bZZqfHliYXfhbY5{fCCqdX3hckBJVDZyIHPlcIx{KGGodHXyJFI1KGi{czDifUBkdG:wb3flcolkKGG\|c3H5MEBNSzVyPU[uN:69VQ>?	NVrOepp4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUixOlM2QDlpPkG4NVY{PTh7PD;hQi=>
L1210	MWfBcpRqdGW3a3XtbYMh[XO\|YYm=		NV3WWHhtOjRiaILz	NIqwO4FCdnSrbHX1b4VucWNiYXP0bZZqfHliYXfhbY5{fCCvb4Xz[UBNOTJzMDDj[YxteyCjZoTldkAzPCCqcoOgZpkh[2yxbn;n[Y5q[yCjc4PhfUwhVEN3ME22MlXPxE1?	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDF4M{W4PUc,OThzNkO1PFk9N2F-
MOLT3	MlnJRY51cWyndXvlcYlkKGG\|c3H5		MlyzNlQhcHK\|	NFvh[nhCdnSrbHX1b4VucWNiYXP0bZZqfHliYXfhbY5{fCCqdX3hckBOV0yWMzDj[YxteyCjZoTldkAzPCCqcoOgZpkh[2yxbn;n[Y5q[yCjc4PhfUwhVEN3ME23MlXPxE1?	NGHwO4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEG2N\|U5QSd-MUixOlM2QDl:L3G+
MOLT3	M4XtOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		Mn7yOFghcHK\|	Mly3S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUW9NXDNiY3XscJMh[W[2ZYKgOFghcHK\|LDDJS\|UxRTF{LkhOwG0>	MnTXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThzNkO1PFkoRjF6MU[zOVg6RC:jPh?=
Raji	NYPkdGhnSW62aXzleYtmdWmlIHHzd4F6		M3HGNVI1KGi{cx?=	MlfDRY51cWyndXvlcYlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gVoFrcSClZXzsd{Bi\nSncjCyOEBpenNiYomgZ4xwdm:pZX7pZ{Bie3OjeTygUGM2OD1zMz63{txO	M2LjTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MU[zOVg6Lz5zOEG2N\|U5QTxxYU6=
HL60	M1f0fmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		MVq0PEBpenN?	MWDHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IES4JIhzeyxiSVe1NF0yQC5{zszN	NWnHSYxORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUixOlM2QDlpPkG4NVY{PTh7PD;hQi=>
L1210	MlTzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NEnxO4EzPCCqcoO=	M37oOmdzd3e2aDDpcohq[mm2aX;uJI9nKG2xdYPlJGwyOjFyIHPlcIx{KGGodHXyJFI1KGi{czygTWc2OD1zOD61{txO	M{LOSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MU[zOVg6Lz5zOEG2N\|U5QTxxYU6=
Raji	NGfBbZhIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		MV60PEBpenN?	M2ruUWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJHJicmliY3XscJMh[W[2ZYKgOFghcHK\|LDDJS\|UxRTF6LkdOwG0>	M2PPOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MU[zOVg6Lz5zOEG2N\|U5QTxxYU6=
KG1a	MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NF3YSGc1QCCqcoO=	NETr[2lIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBMTzGjIHPlcIx{KGGodHXyJFQ5KGi{czygTWc2OD1{M988US=>	M1ewS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MU[zOVg6Lz5zOEG2N\|U5QTxxYU6=
L1210	MVLGeY5kfGmxbjDhd5NigQ>?	M2jETlEhfG9iMkCgeW0>	NW\pZoVqOjRiaILz	M4C3TWlv\HWldHnvckBw\iCyMUXDSGtPOkJicnWt[ZhxemW\|c3nvckBqdiCvb4Xz[UBNOTJzMDDj[YxteyCjdDCxJJRwKDJyIIXNJIFnfGW{IEK0JIhzeyCkeTDSWE1RS1J?	NH\DTIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEG2N\|U5QSd-MUixOlM2QDl:L3G+
HEK293	NFnBVItHfW6ldHnvckBie3OjeR?=			MV7BcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJJJm[2:vYnnuZY51KEy[UnLleIEh\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGlEPTB;MkNOwG0>	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN2M{GyOkc,OTh\|NEOxNlY9N2F-
HEK293	MlX6SpVv[3Srb36gZZN{[Xl?			M{XoW2FvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hemWlb33ibY5idnRiTGjSZYxxcGFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUC9N\|HPxE1?	M1[5W\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{SzNVI3Lz5zOEO0N\|EzPjxxYU6=
NIH3T3	NEPOPFdHfW6ldHnvckBie3OjeR?=			NXz4WZB3SWexbnnzeEBi[3Srdnn0fUBifCCFRmTSMYRmdHSjRkWwPEBufXSjboSg[ZhxemW\|c3XkJIlvKE6LSEPUN{Bk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCob4Lzb49tcW5vc4TpcZVt[XSnZDDjeZJz\W62LDDFR\|UxRTRwNN88US=>	MmDZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3OUW2PVYoRjF6NUm1Olk3RC:jPh?=
NHEM	MV;GeY5kfGmxbjDhd5NigQ>?		M4Sx[FczKGi{cx?=	NX;UdXVwUW6qaXLpeIlwdiCxZjDt[YxidmmwIIP5cpRp\XOrczDpckBPUEWPIHPlcIx{KGG\|c3Xzd4VlKGG\|IGuxOGNefGirb4XyZYNqdCCrbnPvdpBwemG2aX;uJIFnfGW{IEeyJIhzeyCkeTDsbZF2cWRic3PpcpRqdGyjdHnvckBkd3WwdHnu[{whUUN3ME2yPU4z\|ryP	NYfKUHQ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxN\|I6OzRpPkG5NVMzQTN2PD;hQi=>
RAW264.7	NWDqVI4xTnWwY4Tpc44h[XO\|YYm=			MWfJcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIGTOSoFteGijIILlcIVie2ViaX6gcY92e2ViUlHXNlY1NjdiY3XscJMheHKndILlZZRm\CBzIHjyJIJm\m:{ZTDMVHMh[2ijbHzlcodmKGK7IHXufplu\SCrbX31co9ie3OjeTygTWM2OD1{Nj61{txO	M2fYZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7Mke4PFU1Lz5zOUK3PFg2PDxxYU6=
MDA-kb2	NFXyeGFHfW6ldHnvckBie3OjeR?=			M1\NfWFod26rc4SgZYN1cX[rdImgZZQh[W6mcn;n[Y4hemWlZYD0c5IhcW5iaIXtZY4hVUSDLXviNkBk\WyuczDhd5Nme3OnZDDhd{B{fGmvdXzheIlwdiCxZjDseYNq\mW{YYPlJIFkfGm4aYT5JIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWMyPTB;ND60{txO	MnnDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3OUKyOFUoRjF7NUmyNlQ2RC:jPh?=
MDA-kb2	NYK0c21qTnWwY4Tpc44h[XO\|YYm=			MlLJRYdwdmm\|dDDhZ5Rqfmm2eTDheEBodHWlb3PvdpRq[2:rZDDy[YNmeHSxcjDpckBpfW2jbjDNSGEuc2J{IHPlcIx{KGG\|c3Xzd4VlKGG\|IIP0bY12dGG2aX;uJI9nKGy3Y3nm[ZJie2ViYXP0bZZqfHliYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{G1NF01NjUQvF2=	Ml\ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3OUKyOFUoRjF7NUmyNlQ2RC:jPh?=
MCF7	MnjsRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?			MoPsRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDld5Rzd2enbjDy[YNmeHSxcjDlfJBz\XO\|aX7nJIh2dWGwIF3DSlch[2WubIOsJGdKPTB;MUFOwG0>	M4rscFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OEG4OlEzLz5zOUixPFYyOjxxYU6=
MDA-MB-436	MoHlRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?			M2j4emFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[ZN1em:pZX6gdoVk\XC2b4Kg[ZhxemW\|c3nu[{BpfW2jbjDNSGEuVUJvNEO2JINmdGy\|LDDHTVUxRTF5zszN	M{HpUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OEG4OlEzLz5zOUixPFYyOjxxYU6=
FRT	MUDGeY5kfGmxbjDhd5NigQ>?			M1rURWJqdmSrbnegZYZncW6rdImgeI8hS0[WUjDk[Yx1[UZ3MEigcZV1[W62IHX4dJJme3OnZDDpckBHWlRiY3XscJMh[2:neIDy[ZN{cW6pIHjhcIll\SC\|ZX7zbZRqfmViWV\QMWgyPDiTL1mxOVJNKG23dHHueEBxem:2ZXnuJIJ6KGmxZHnk[UBqdm[udYigZZN{[XluIFvkQVE3NjgQvF2=	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh6MEOyN{c,OTl6OECzNlM9N2F-
T47D	MUHFd5Rzd2enbnnjJIF{e2G7		M{XQeVk3KGi{cx?=	NULNSYRXTXO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCWNEfEJINmdGy\|IHHzd4V{e2WmIHHzJIRzfWdibHX2[Ywh[2G3c3nu[{B{fGmvdXzheIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36g[ZF2cX[jbHXueEB1dyBzMDDwUUBme3S{YXTpc4wh[W[2ZYKgPVYhcHK\|IHL5JIFt[W2jcjDicJVmKGG\|c3H5MEBC[3Srdnn0fV0xNjBzzszN	NXq3WYJuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5Nlg5OzJpPkG5PVI5QDN{PD;hQi=>
MCF7	MX3Fd5Rzd2enbnnjJIF{e2G7		NVfBN\|F2QTZiaILz	NIXOSYVGe3S{b3flcolkKGGldHn2bZR6KGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOg[JJ2\yCuZY\lcEBk[XW\|aX7nJJN1cW23bHH0bY9vKG:oIHPlcIwheHKxbHnm[ZJifGmxbjDldZVqfmGuZX70JJRwKDFyIIDNJIV{fHKjZHnvcEBi\nSncjC5OkBpenNiYomgZYxidWG{IHLseYUh[XO\|YYmsJGFkfGm4aYT5QVAvODIQvF2=	NY[4b45YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5Nlg5OzJpPkG5PVI5QDN{PD;hQi=>
MCF7	NXexR\|l3TXO2cn;n[Y5q[yCjc4PhfS=>			NF:2T4pGe3S{b3flcolkKGGldHn2bZR6KGmwIHz1Z4ln\XKjc3WgeJJidnOoZXP0[YQhcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyCmcoXnJIxmfmWuIHPheZNqdmdic4TpcZVt[XSrb36gc4Yh[2WubDDwdo9tcW[ncnH0bY9vKGWzdXn2ZYxmdnRidH:gNVAheE1iZYP0doFlcW:uIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiQXP0bZZqfHl;MD6wNe69VQ>?	NGToSIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUmyPFg{Oid-MUm5Nlg5OzJ:L3G+
MCF7	NH;zdW9Ge3S{b3flcolkKGG\|c3H5			MoDwSZN1em:pZX7pZ{Bi[3Srdnn0fUBqdiCudXPp[oVz[XOnIITyZY5{\mWldHXkJIh2dWGwIF3DSlch[2WubIOgZZN{\XO\|ZXSgZZMh\HK3ZzDs[ZZmdCClYYXzbY5oKHO2aX31cIF1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBmeXWrdnHs[Y51KHSxIEGwNEBxVSCnc4TyZYRqd2xiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCDY4Tpeol1gT1yLkCx{txO	M{Hl[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OUK4PFMzLz5zOUmyPFg{OjxxYU6=
T47D	NFHqR\|hGe3S{b3flcolkKGG\|c3H5			Mm\BSZN1em:pZX7pZ{Bi[3Srdnn0fUBqdiCudXPp[oVz[XOnIITyZY5{\mWldHXkJIh2dWGwIGS0O2Qh[2WubIOgZZN{\XO\|ZXSgZZMh\HK3ZzDs[ZZmdCClYYXzbY5oKHO2aX31cIF1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBmeXWrdnHs[Y51KHSxIEGwJJBOKGW\|dILh[IlwdCCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEGldHn2bZR6RTBwMEJOwG0>	MofuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Mki4N\|IoRjF7OUK4PFMzRC:jPh?=
T47D	M1HDcWV{fHKxZ3XubYMh[XO\|YYm=		M1\LeFk3KGi{cx?=	M2H1NGV{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hXDR5RDDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegd5RqdXWuYYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:wIHXxeYl3[WynboSgeI8hOTByIIDNJIV{fHKjZHnvcEBi\nSncjC5OkBpenNiYomgZYxidWG{IHLseYUh[XO\|YYmsJGFkfGm4aYT5QVAvODkQvF2=	NGq1PJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUmyPFg{Oid-MUm5Nlg5OzJ:L3G+
MCF7	NGGzdHlGe3S{b3flcolkKGG\|c3H5		MX:5OkBpenN?	M1[4PWV{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegd5RqdXWuYYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:wIHXxeYl3[WynboSgeI8hOTByIIDNJIV{fHKjZHnvcEBi\nSncjC5OkBpenNiYomgZYxidWG{IHLseYUh[XO\|YYmsJGFkfGm4aYT5QVAvOTIQvF2=	Ml\nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Mki4N\|IoRjF7OUK4PFMzRC:jPh?=
T47D	MlLRSZN1em:pZX7pZ{Bie3OjeR?=			M2n2e2V{fHKxZ3XubYMh[WO2aY\peJkhcW5ibIXjbYZmemG\|ZTD0doFve2[nY4Tl[EBpfW2jbjDUOFdFKGOnbHzzJIF{e2W\|c3XkJIF{KGS{dXegcIV3\WxiY3H1d4lv\yC\|dHnteYxifGmxbjDv[kBk\WyuIIDyc4xq\mW{YYTpc44h\XG3aY\hcIVvfCC2bzCxNFAheE1iZYP0doFlcW:uIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiQXP0bZZqfHl;MD62{txO	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl{OEizNkc,OTl7Mki4N\|I9N2F-
RAW264.7	NXH0WWdQSW62aXnu[oxidW2jdH;yfUBie3OjeR?=		NGi4TGwzPCCqcoO=	MmrFRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBNWFNvc4TpcZVt[XSnZDDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJI5qfHKrYzDvfIll\SCycn;keYN1cW:wIHHmeIVzKDJ2IHjyd{BjgSCJcnnld5MhemWjZ3XueEwhUUN3ME2yOu69VQ>?	MkjuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB\|NkOxOFUoRjJyM{[zNVQ2RC:jPh?=
MCF7	MYPDfZRwfG:6aXPpeJkh[XO\|YYm=		M3XXXVQ5KGi{cx?=	MnP6R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyPUJOwG0>	MmLpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6MUO1NlQoRjJyOEGzOVI1RC:jPh?=
LoVo	M{fxPWN6fG:2b4jpZ4l1gSCjc4PhfS=>		NUHabmJSPzJiaILz	MX3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMc3ZwKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MUWuPFjPxE1?	NH7aXlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUGyPVk4Pyd-MkGxNlk6Pzd:L3G+
LNCAP	NFi1Z2REgXSxdH;4bYNqfHliYYPzZZk>		MnnKO\|IhcHK\|	MVPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMUmNCWCClZXzsd{Bi\nSncjC3NkBpenNiYomgV3JDKGG\|c3H5MEBKSzVyPUOwMlY2\|ryP	NWPGW4lYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxNlk6PzdpPkKxNVI6QTd5PD;hQi=>
HCT116	NYDQeGR4S3m2b4TvfIlkcXS7IHHzd4F6		MYK3NkBpenN?	NYDUOFlMS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUG2JINmdGy\|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OzRwOd88US=>	NWnLW\|JORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxNlk6PzdpPkKxNVI6QTd5PD;hQi=>
AGS	NE\PRlZEgXSxdH;4bYNqfHliYYPzZZk>		MlvlO\|IhcHK\|	MoT3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRWdUKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;NEGuOlfPxE1?	NH3QPXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUGyPVk4Pyd-MkGxNlk6Pzd:L3G+
A549	MY\DfZRwfG:6aXPpeJkh[XO\|YYm=		MnSwO\|IhcHK\|	NVTDPVZuS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVQ{NjB7zszN	NI\VdHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUGyPVk4Pyd-MkGxNlk6Pzd:L3G+
DU145	MYXDfZRwfG:6aXPpeJkh[XO\|YYm=		Mlm5O\|IhcHK\|	MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDEWVE1PSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG\|c3H5MEBKSzVyPUS3MlI6\|ryP	M1;JUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUK5PVc4Lz5{MUGyPVk4PzxxYU6=
RAW264.7	M4qxemFvfGmrbn\sZY1u[XSxcomgZZN{[Xl?		M1TIPFI1KGi{cx?=	NVzwWnQ3SW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJHRPTmGucHjhJJBzd2S3Y4Tpc44hfHKnYYTl[EAzKGi{czDi[YZwemViTGDTJINp[WyuZX7n[UBu\WG\|dYLl[EBi\nSncjCyOEBpenNiYomgSWxKW0FuIFnDOVA:OTlwMd88US=>	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ6OEeyO{c,OjF{OEi3Nlc9N2F-
MDCK	M2rnOmZ2dmO2aX;uJIF{e2G7			NV;Wc\|drUW6qaXLpeIlwdiCxZjDCR3JRKGW6cILld5Nm\CCrbjDNSGNMKGOnbHzzJJV{cW6pIFjv[YNpe3RiM{OzOFIhe3SjaX7pcoctKEmFNUC9Ok46\|ryP	MlOzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF\|NUS4NFAoRjJzM{W0PFAxRC:jPh?=
MCF-7 MX	MUnGeY5kfGmxbjDhd5NigQ>?			MVTJcohq[mm2aX;uJI9nKEKFUmCg[ZhxemW\|c3XkJIlvKE2FRj23JG1ZKGOnbHzzJJV{cW6pIFjv[YNpe3RiM{OzOFIhe3SjaX7pcoctKEmFNUC9PE45\|ryP	NV;RcVZjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzOVQ5ODBpPkKxN\|U1QDByPD;hQi=>
NCI-ADR-RES	M{TUSWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NUXETYxyPDhiaILz	MYXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCQQ1mtRWRTNVKHUzDj[YxteyCrbnP1ZoF1\WRid3n0bEAxNjB4IH7NJI9nKGW\|dILh[IlwdCCjbnSgNE4xPyCwTTDv[kB1\XO2b4P0[ZJwdmViYX\0[ZIhPDhiaILzJIJ6KHO3bH\vdohw\GGvaX7lJGIh[XO\|YYmgbY4heHKnc3XuZ4Uhd2ZiZnX0ZYwh[m:4aX7lJJNmenWvIHHu[EBPfVOncoXtMEBIUTVyPUG2Mlk5OjUQvF2=	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO\|U9N2F-
MCF12A	MlHJRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NUf3bG5pPDhiaILz	MVzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCPQ1[xNmEh[2WubIOgbY5kfWKjdHXkJJdqfGhiMD6wOkBvVSCxZjDld5Rz[WSrb3ygZY5lKDBwMEegcm0hd2ZidHXzeI9{fGW{b37lJIFnfGW{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJIZmfGGuIHLveolv\SC\|ZYL1cUBidmRiToXT[ZJ2dSxiR1m1NF0zOS56N{e2{txO	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO\|U9N2F-
NCI-ADR-RES	NF;adXdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M3PxV\|Q5KGi{cx?=	NUKx[HViSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBme3S{b3flckBz\WOncITvdk1l\W[rY3nlcpQhcHWvYX6gUmNKNUGGUj3SSXMh[2WubIOgbY5kfWKjdHXkJJdqfGhiMUCwJI5OKG:oIHXzeJJi\GmxbDDhcoQhOC5yNzDuUUBw\iC2ZYP0c5N1\XKxbnWgZYZ1\XJiNEigbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJiYYPzZZkhcW5icILld4Vv[2Vib3[g[oV1[WxiYn;2bY5mKHOncoXtJIFv\CCQdWPldpVuNCCJSUWwQVI3NjlzNUVOwG0>	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO\|U9N2F-
MCF12A	NVnwdJpzSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MV[0PEBpenN?	MVXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCPQ1[xNmEh[2WubIOgbY5kfWKjdHXkJJdqfGhiMUCwJI5OKG:oIHXzeJJi\GmxbDDhcoQhOC5yNzDuUUBw\iC2ZYP0c5N1\XKxbnWgZYZ1\XJiNEigbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJiYYPzZZkhcW5icILld4Vv[2Vib3[g[oV1[WxiYn;2bY5mKHOncoXtJIFv\CCQdWPldpVuNCCJSUWwQVMxNjF7OUZOwG0>	M1XMZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlc2Lz5{MUWxN\|I4PTxxYU6=
NCI-ADR-RES	NWnJUmhbSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NFzqOVA1QCCqcoO=	MVzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCQQ1mtRWRTNVKHUzDj[YxteyCrbnP1ZoF1\WRid3n0bEAxNjByMzDuUUBw\iCnc4TyZYRqd2xiYX7kJFAvODFibl2gc4YhfGW\|dH;zeIVzd26nIHHmeIVzKDR6IHjyd{BjgSC\|dXzmc5Jpd2SjbXnu[UBDKGG\|c3H5JIlvKHC{ZYPlcoNmKG:oIEWlJIZmfGGuIHLveolv\SC\|ZYL1cUwhT0l3ME2zNk4{PTl2zszN	NGnxUG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxN\|I4PSd-MkG1NVMzPzV:L3G+
MCF12A	NEnkZlVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		MoWxOFghcHK\|	MWfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCPQ1[xNmEh[2WubIOgbY5kfWKjdHXkJJdqfGhiMD6wNFMhdk1ib3[g[ZN1emGmaX;sJIFv\CByLkCxJI5OKG:oIITld5Rwe3Sncn;u[UBi\nSncjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSCrbjDwdoV{\W6lZTDv[kA2LSCoZYThcEBjd3[rbnWgd4VzfW1uIFfJOVA:OzJwM{W5OO69VQ>?	NF\wW4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxN\|I4PSd-MkG1NVMzPzV:L3G+
LNCAP	NXPPeHQ2SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NELkZoE1QCCqcoO=	MWrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEyQQ1HQJINmdGy\|IHX4dJJme3OrbnegZY5lem:pZX6gdoVk\XC2b4KgbY5kfWKjdHXkJJdqfGhiMD62JI5OKG:oIHXzeJJi\GmxbDDhcoQhOC55IH7NJI9nKHSnc4Tvd5Rmem:wZTDh[pRmeiB2ODDodpMh[nlic4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUwhT0l3ME2zPE46ODR3zszN	NV3leVc4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzPzVpPkKxOVE{Ojd3PD;hQi=>
PC3	MUPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NVLsfY5NPDhiaILz	NXvTO\|BjSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBidmS{b3flckBz\WOncITvdk1l\W[rY3nlcpQhcHWvYX6gVGM{KGOnbHzzJIlv[3WkYYTl[EB4cXSqIECuOkBvVSCxZjDld5Rz[WSrb3ygZY5lKDBwNzDuUUBw\iC2ZYP0c5N1\XKxbnWgZYZ1\XJiNEigbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJiYYPzZZktKEeLNUC9OFIvPjV5Od88US=>	MmS5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUOyO\|UoRjJzNUGzNlc2RC:jPh?=
MCF7	M2HReGFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MUK0PEBpenN?	M1fFWWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDlfJBz\XO\|aX7nJIV{fHKxZ3XuJJJm[2WydH;yJIlv[3WkYYTl[EB4cXSqIECuNFYhdk1ib3[g[ZN1emGmaX;sJIFv\CByLkC3JI5OKG:oIITld5Rwe3Sncn;u[UBi\nSncjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSCrbjDwdoV{\W6lZTDv[kBn\XSjbDDic5ZqdmVic3XyeY0h[W6mIF71V4VzfW1uIFfJOVA:PDVwN{C4PO69VQ>?	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO\|U9N2F-
FRT	NYW0W41pTnWwY4Tpc44h[XO\|YYm=		NFrQVZkzPCCqcoO=	NHnBbHRDcW6maX7nJIFn\mmwaYT5JJRwKEOIVGKgSlUxQCCmZXzleIlwdiCvdYThcpQh\XiycnXzd4VlKGmwIH\vdpNsd2yrbj3zeIlufWyjdHXkJGZTXCClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDpc4RqdmViaX7mcJV5KG2nYYP1doVlKGG\|IGnGVEByfWWwY3jpcochemG2ZTDh[pRmeiB{NDDodpMh[nliZnz1c5Jme2OnbnPlJIF{e2G7LDDL[F06NjUQvF2=	NEO3boQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUW2PFMzOyd-MkG1Olg{OjN:L3G+
MCF7	M1fXO2FvfGmnc4Tyc4dmdmmlIHHzd4F6	M1L6bVEhfG9iMkCgeW0>	NFr2Z201QCCqcoO=	MX7BcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCHUj3wc5NqfGm4ZTDNR2Y4KGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gNVdj\XSjIHXzeJJi\GmxbD3pcoR2[2WmIIPlZ5JmfGWmIHHsb4FtcW6nIIDoc5NxcGG2YYPlJIFkfGm4aYT5JIF1KDFidH:gNlAhfU1iYX\0[ZIhPDhiaILzJIJ6KHCqb4PwbIEudGmpaISgdoVxd3K2ZYKgZ4hmdWmudX3pcoV{[2WwY3WgZZN{[Xl?	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOThyMEi1PUc,OjF6MEC4OVk9N2F-
Jurkat T	MorERZBweHSxc3nzJIF{e2G7		MnrBN\|YhcHK\|	MmP2TY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCMdYLrZZQhXCClZXzsd{BwfmW{ZYjwdoV{e2mwZzDO[Y8h[W[2ZYKgN\|YhcHK\|IHL5JIZtd3diY4n0c41mfHK7	NYLuUIlyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxPVc{QTNpPkKyNVk4Ozl\|PD;hQi=>
FRT	Mk\tSpVv[3Srb36gZZN{[Xl?			M1\hRXBwfGWwdHnheIlwdiCjY4Tpeol1gSCjdDDoeY1idiCFRmTSJIRmdHSjIF[1NFghdXW2YX70MY1m\GmjdHXkJIlw\GmwZTDmcJV5KGW6cILld5Nm\CCrbjDyZZQhTlKWIHPlcIx{KGOxZYjwdoV{e2mwZzDmcJVwemW\|Y3XueEBxem:2ZXnuJHlHWC2KMUS4VU8yOTV{TDDifUBndHWxcnXzZ4Vv[2ViYYPzZZktKEWFNUC9O:69VQ>?	M1PhVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkixNVg2Lz5{MkK4NVE5PTxxYU6=
Human Huh7.5.1	NV;lTVRqSW62aY\pdoFtKGG\|c3H5		NHi4PJc4OiCqcoO=	NHLHZWdCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJS1ZiSl\IMVEhUjN7OVXNJIlv\mWldHXkJIlvKEi3bXHuJGh2cDdwNT6xJINmdGy\|IHHzd4V{e2WmIHHzJJN2eHC{ZYPzbY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wIHHmeIVzKDd{IHjyd{BjgSCHR1\QJIF{e2G7LDDFR\|UxRTF2LkVOwG0>	M2TjNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NES1N\|I5Lz5{MkS0OVMzQDxxYU6=
CHO	M3m1TmZ2dmO2aX;uJIF{e2G7			NFL1SIhMcSC4YXz1[ZMh\m:{IIPv[Il2dSCobIXvdoV{[2WrbjCoNVAhfU1rIIXweIFs\SCrbjDPRXRROUJ\|LYTyZY5{\mWldHXkJGNJVyClZXzsd{whU2l;Mj65Ou69VQ>?	M{jkeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NUexOFE2Lz5{M{W3NVQyPTxxYU6=
CHO	MWHGeY5kfGmxbjDhd5NigQ>?			MYDwTWM2OCC4YXz1[ZMh\m:{IIPv[Il2dSCobIXvdoV{[2WrbjCoNVAhfU1rIIXweIFs\SCrbjDPRXRROUJ\|LYTyZY5{\mWldHXkJGNJVyClZXzsd{whUUN3ME2zMlg6ODR3zszN	NUHxfYtZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1O\|E1OTVpPkKzOVcyPDF3PD;hQi=>
CHO	NY\IS2J6TnWwY4Tpc44h[XO\|YYm=			NYXPNmZ3U2lidnHseYV{KG[xcjDzc4RqfW1iZnz1c5Jme2OnaX6gLFExKHWPKTD1dJRic2ViaX6gU2FVWDGEMT30doFve2[nY4Tl[EBEUE9iY3XscJMtKEurPUZOwG0>	NX:yZW9YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1O\|E1OTVpPkKzOVcyPDF3PD;hQi=>
CHO	MXPGeY5kfGmxbjDhd5NigQ>?			MkC0dGlEPTBidnHseYV{KG[xcjDzc4RqfW1iZnz1c5Jme2OnaX6gLFExKHWPKTD1dJRic2ViaX6gU2FVWDGEMT30doFve2[nY4Tl[EBEUE9iY3XscJMtKEmFNUC9PU4yOjBzMd88US=>	M4XWS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NUexOFE2Lz5{M{W3NVQyPTxxYU6=
HuH7	MlXKRY51cXCuYYPtc4Rq[WxiYYPzZZk>		NFf4WGQzPCCqcoO=	NVXuVHh7SW62aYDsZZNud2SrYXygZYN1cX[rdImgZYdicW6\|dDDsbZZmeiC\|cH;yc5pwcXSnIIP0ZYdmKG:oIGDsZZNud2SrdX2gZoVz\2inaTDBUmtCKGW6cILld5NqdmdiR1\QJIlv\mWldHXkJIlvKGi3bXHuJGh2UDdiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBx[XKjc3n0[UBl\X[nbH;wcYVvfCCjZoTldkAzPCCqcoOgZpkheVKWLWDDVkBidmGueYPpd{whUUN3ME2yNO69VQ>?	NWW1dIdURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNlU5PDlpPkK0NVI2QDR7PD;hQi=>
SKBR3	NGXqdmNE\WyuIHP5Z4xmKGG\|c3H5	M{DwbVUxKHSxIEe1JJVO	NWnDPGxyOzZiaILz	NH7VZphE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBUU0KUMzDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG\|ZTDpckBi[2O3bYXsZZRqd25iYYSgS\|AwTzFicHjhd4Uh[XRiNUCgeI8hPzVidV2gZYZ1\XJiM{[gbJJ{KGK7IIDyc5Bq\Gm3bTDpc4Rq\GVic4ThbY5qdmdvYnHz[YQh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nz	NG\6XoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES1OlAxPCd-MkS0OVYxODR:L3G+
A431	MYnD[YxtKGO7Y3zlJIF{e2G7	NVy3bZlkPTBidH:gO\|UhfU1?	MlzNN\|YhcHK\|	MWTD[YxtKGO7Y3zlJIFzemW\|dDDpckBpfW2jbjDBOFMyKGOnbHzzJIF{e2W\|c3XkJIF{KGSnY4LlZZNmKGmwIHHjZ5VufWyjdHnvckBifCCJMD;HNUBxcGG\|ZTDheEA2OCC2bzC3OUB2VSCjZoTldkA{PiCqcoOgZpkheHKxcHnkbZVuKGmxZHnk[UB{fGGrbnnu[{1j[XOnZDDmcI94KGO7dH;t[ZRzcWNiYX7hcJl{cXN?	NGDUO4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES1OlAxPCd-MkS0OVYxODR:L3G+
A431	NYS0T\|h5S2WubDDjfYNt\SCjc4PhfS=>	NGH0Wpo2OCC2bzC3OUB2VQ>?	NV;iW3dpOzZiaILz	M4XHV2NmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJGE1OzFiY3XscJMh[XO\|ZYPz[YQh[XNiYXPjeY12dGG2aX;uJIF1KEd{L12gdIhie2ViYYSgOVAhfG9iN{WgeW0h[W[2ZYKgN\|YhcHK\|IHL5JJBzd3CrZHn1cUBqd2SrZHWgd5RicW6rbnetZoF{\WRiZnzve{BkgXSxbXX0dolkKGGwYXz5d4l{KHKnbHH0bZZmKHSxIHPvcpRzd2x?	Mn\2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NU[wNFQoRjJ2NEW2NFA1RC:jPh?=
A431	NHfNbm5CeG:ydH;zbZMh[XO\|YYm=	Mlf0OVAhfG9iN{WgeW0>	MWe3NkBpenN?	NIn3eJBKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m\|IHnuJIh2dWGwIFG0N\|Eh[2WubIOgc5ZmemW6cILld5NqdmdiRYLiRkBqdiClb33wcIV1\SCvZXTpeY0h[XO\|ZYPz[YQh[XNibHH0[UBieG:ydH;0bYMwdmWlcn;0bYMh[2WubIOgZZQhPTBidH:gO\|UhfU1iYX\0[ZIhPzJiaILzJIJ6KGGwbnX4bY4hXi2ISWTDM\|cuSUGGIIP0ZYlvcW6pLXLhd4VlKG[ub4egZ5l1d22ndILpZ{BidmGueYPpdy=>	NFjzWo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES1OlAxPCd-MkS0OVYxODR:L3G+
MCF7	M1HsfGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	NXTJVmpIOC5zIITvJFIxKHWP	M2i1W\|Qh\GG7cx?=	NXTsc2YxW3SrbYXsZZRqd25ib3[g[5Jwf3SqIHnuJIh2dWGwIF3DSlch[2WubIOgZZQhOC5zIITvJFIxKHWPIHHmeIVzKDRiZHH5d{BjgSCPVGSgZZN{[Xl?	MljrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyN{izNVQoRjJ3MEe4N\|E1RC:jPh?=
MCF7	NFjUUZlIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	NU[wXIpTOjBidH:gNVAxKHWP	NGnw[2U1KGSjeYO=	NFT5PZhIem:5dHigbY5pcWKrdHnvckBqdiCqdX3hckBOS0Z5IHPlcIx{KGG2IEKwJJRwKDFyMDD1UUBi\nSncjC0JIRigXNiYomgUXRVKGG\|c3H5	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB5OEOxOEc,OjVyN{izNVQ9N2F-
RAW264.7	NWnYfndXTnWwY4Tpc44h[XO\|YYm=	M{LxN\|I3KHWP		MVnJcohq[mm2aX;uJI9nKG6rdILpZ{BwgGmmZTDwdo9lfWO2aX;uJIlvKEySUz;JSm5o[W2vYT3zeIlufWyjdHXkJI1wfXOnIGLBW\|I3PC55IHPlcIx{KGG\|c3Xzd4VlKGG\|IH7peJJqfGW\|IHzleoVtKGG2IEK2JJVOKGK7IFfybYV{eyCvZYToc4QhMFK4YjC9JFYvPCBtLz2gNE42KHWPKTygRYN1cX[rdIm9Ok4y\|ryP	NESzSpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyO\|E2Oyd-MkWxNlcyPTN:L3G+
RAW264.7	NWjzR4JxTnWwY4Tpc44h[XO\|YYm=	M{LTSFYvPSC3TR?=		M{n4dGlvcGmkaYTpc44hd2Zibnn0dolkKG:6aXTlJJBzd2S3Y4Tpc44hcW5iTGDTM2lHVmejbX3hMZN1cW23bHH0[YQhdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgcol1emm2ZYOgcIV3\WxiYYSgOk42KHWPIHL5JGdzcWW\|czDt[ZRpd2RiKGL2ZkA:KDZwNDCrM{0hOC53IIXNLUwhSWO2aY\peJk:Pi5\|zszN	NUnWNldGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlcyPTNpPkK1NVI4OTV\|PD;hQi=>
RAW264.7	MoTuSpVv[3Srb36gZZN{[Xl?	NXy0coNnOTNidV2=		M3P1emlvcGmkaYTpc44hd2Zibnn0dolkKG:6aXTlJJBzd2S3Y4Tpc44hcW5iTGDTM2lHVmejbX3hMZN1cW23bHH0[YQhdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgcol1emm2ZYOgcIV3\WxiYYSgNVMhfU1iYomgS5Jq\XO\|IH3leIhw\CBqUo\iJF0hPi52IDuvMUAxNjVidV2pMEBC[3Srdnn0fV03NjQQvF2=	M{KzXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUK3NVU{Lz5{NUGyO\|E2OzxxYU6=
J774A1	NFzSe\|dHfW6ldHnvckBie3OjeR?=	NEHFfXg3NjVidV2=		NFrtNHpKdmirYnn0bY9vKG:oIH7peJJq[yCxeHnk[UBxem:mdXP0bY9vKGmwIFzQV{9KTk6pYX3tZU1{fGmvdXzheIVlKG2xdYPlJGo4PzSDMTDj[YxteyCjc4Pld5Nm\CCjczDubZRzcXSnczDs[ZZmdCCjdDC2MlUhfU1iYomgS5Jq\XO\|IH3leIhw\CBqUo\iJF0hOTZwNTCrM{0hOS52IIXNLUwhSWO2aY\peJk:OTNwNN88US=>	NVrUT4pMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlcyPTNpPkK1NVI4OTV\|PD;hQi=>
J774A1	Mn7CSpVv[3Srb36gZZN{[Xl?	MorVNVMhfU1?		MofITY5pcWKrdHnvckBw\iCwaYTybYMhd3irZHWgdJJw\HWldHnvckBqdiCOUGOvTWZP\2GvbXGtd5RqdXWuYYTl[EBud3W\|ZTDKO\|c1STFiY3XscJMh[XO\|ZYPz[YQh[XNibnn0dol1\XNibHX2[Ywh[XRiMUOgeW0h[nliR4Lp[ZN{KG2ndHjv[EApWn[kIE2gNVYvPSBtLz2gNU41KHWPKTygRYN1cX[rdIm9NVMvQM7:TR?=	M3PkclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUK3NVU{Lz5{NUGyO\|E2OzxxYU6=
J774A1	M37kVmZ2dmO2aX;uJIF{e2G7	MmjUNlYhfU1?		M13INWlvcGmkaYTpc44hd2Zibnn0dolkKG:6aXTlJJBzd2S3Y4Tpc44hcW5iTGDTM2lHVmejbX3hMZN1cW23bHH0[YQhdW:3c3WgTlc4PEFzIHPlcIx{KGG\|c3Xzd4VlKGG\|IH7peJJqfGW\|IHzleoVtKGG2IEK2JJVOKGK7IFfybYV{eyCvZYToc4QhMFK4YjC9JFE3NjViKz:tJFEvPCC3TTmsJGFkfGm4aYT5QVE1NjYQvF2=	M3;KS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUK3NVU{Lz5{NUGyO\|E2OzxxYU6=
HEK293	NVvWeXRwTnWwY4Tpc44h[XO\|YYm=			NFnQ[XJKdmirYnn0bY9vKG:oIFXHSnIhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W\|c3XkJIF{KGSnY4LlZZNmKGmwIIDoc5NxcG:{eXzheIlwdiCkeTDjbIVucWy3bXnu[ZNk\W6lZTDhd5NigSxiSVO1NF0zPS5zMt88US=>	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJyNUG5NEc,OjV{MEWxPVA9N2F-
Sf9	MUXGeY5kfGmxbjDhd5NigQ>?			MnjlTY5pcWKrdHnvckBw\iCKaYO2MZRi\2enZDDoeY1idiC{ZXPvcYJqdmGwdDDEUm1VOSCneIDy[ZN{\WRiaX6gbY5{\WO2IGPmPUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iRF7BJI1mfGi7bITyZY5{\mW{YYPlJIFkfGm4aYT5JJV{cW6pIEWnMYJqd3SrbonsZZRm\CB2NT3idEB2dm2ndHj5cIF1\WRib4KgbIVucW2ndHj5cIF1\WRib3zp[49vfWOuZX;0bYRmKHO3YoP0doF1\XNiYX7kJHs{UF1vQXTvUYV1KGK7IHzpdZVq\CC\|Y3nueIltNCCLQ{WwQVMx\|ryP	NFH1[HA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSwOlk1PCd-MkW0NFY6PDR:L3G+
LNCAP	MXjDfZRwfG:6aXPpeJkh[XO\|YYm=		MXOzJIRigXN?	MlGyR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gZY5lem:pZX6t[IVx\W6mZX70JGxPS0GSIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4Yh[2WubDD2bYFjcWyrdImgZYZ1\XJiMzDkZZl{KGK7IGfTWE0yKGOnbHygdJJwdGmoZYLheIlwdiCjc4PhfUwhUUN3ME2zO{41\|ryP	M1zqNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{SxNVM2Lz5{NkO0NVE{PTxxYU6=
insect cells	M1\K[GZ2dmO2aX;uJIF{e2G7		M{HEUFExKG2rboO=	MYHJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKE2DTz3BJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3czDpcoZm[3SnZDDCWGkhcW6\|ZXP0JINmdGy\|IIDy[Ylv[3WkYYTl[EBnd3JiMUCgcYlveyCob3zsc5dm\CCkeTDh[IRqfGmxbjDv[kBsgW63cnHtbY5mKGG\|IIP1ZpN1emG2ZTygTWM2OD1\|LkpOwG0>	NIj3NIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{W3OVQ4Pid-Mke1O\|U1PzZ:L3G+
insect cells	M1;hUGZ2dmO2aX;uJIF{e2G7		MYixNEBucW6\|	M1y3TGlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iTVHPMWIh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzJIlv\mWldHXkJGJVUSCrboPlZ5Qh[2WubIOgdJJmcW6ldXLheIVlKG[xcjCxNEBucW6\|IH\vcIxwf2WmIHL5JIFl\Gm2aX;uJI9nKGKnbor5cIFucW6nIHHzJJN2[nO2cnH0[UwhUUN3ME20MlHPxE1?	M4TFblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUe1OFc3Lz5{N{W3OVQ4PjxxYU6=
insect cells	M2TPb2Z2dmO2aX;uJIF{e2G7		M1XqfFQxKG2rboO=	NWXRdnVJUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDNRW8uSiCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhSlSLIHnud4VkfCClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDhdoJqfHKjcomgcIlocHRidX7peJMheHKnaX7jeYJifGWmIH\vdkA1OCCvaX7zJIZwdGyxd3XkJIJ6KGGmZHn0bY9vKG:oIHz1Z4ln\XKrbjDk[ZJqfmG2aY\lJJN2[nO2cnH0[UBu\WG\|dYLl[EBi\nSncjCyJIhzeyCkeTDNRW8uT2yxdzDhd5NigSxiSVO1NF03NjkQvF2=	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzV5NUS3Okc,Ojd3N{W0O\|Y9N2F-
insect cells	M3O4dmZ2dmO2aX;uJIF{e2G7		MlvQOFAhdWmwcx?=	NEL0ZlBKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIF3BU{1CKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EBDXEliaX7z[YN1KGOnbHzzJIF{e2W\|c3XkJIF{KGSnY4LlZZNmKGmwIHHyZol1emG{eTDsbYdpfCC3bnn0d{BxemWrbnP1ZoF1\WRiZn;yJFQxKG2rboOg[o9tdG:5ZXSgZpkh[WSmaYTpc44hd2ZibIXjbYZmemmwIHTldol3[XSrdnWgd5Vje3S{YYTlJI1m[XO3cnXkJIFnfGW{IEKgbJJ{KGK7IF3BU{1IdG:5IHHzd4F6NCCLQ{WwQVkvP87:TR?=	M1fxO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUe1OFc3Lz5{N{W3OVQ4PjxxYU6=
RAW264.7	NHTHdHBCdnSrb4P0[Y9xd3KxdHnjJIF{e2G7		MUS0JJRwKDViZHH5dy=>	NWXLXndISW62aX;zeIVweG:{b4TpZ{Bi[3Srdnn0fUBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGLBUmtNNWmwZIXj[YQhd3O2ZX;jcIF{fCCmaX\m[ZJmdnSrYYTpc44h[W[2ZYKgOEB1dyB3IHThfZMh[nliVGLBVEBie3OjeTygTWM2OD1zMT60{txO	MonFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzNkm1N\|coRjJ6MU[5OVM4RC:jPh?=
SK-MEL-2	M2fKSGN6fG:2b4jpZ4l1gSCjc4PhfS=>		NXvEbIhHPDhiaILz	NXfWZlc{S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0tvTVXMMVIh[2WubIOgZZN{\XO\|ZXSgZZMh\GWlcnXhd4UhcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK\|IHL5JG1VXCCjc4PhfUwhUUN3ME2zOu69VQ>?	M3LVNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NkW0NlY2Lz5{OE[1OFI3PTxxYU6=
MML1	NXrTeFBFS3m2b4TvfIlkcXS7IHHzd4F6		M4LDcFQ5KGi{cx?=	M4WxO2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1OVDFiY3XscJMh[XO\|ZYPz[YQh[XNiZHXjdoVie2ViaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF01Os7:TR?=	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ3NEK2OUc,Ojh4NUSyOlU9N2F-
MDA-MB-231	NHPISmREgXSxdH;4bYNqfHliYYPzZZk>		NUK5fVV{PDhiaILz	NYe0PXRoS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVQ{\|ryP	M1zaclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NkW0NlY2Lz5{OE[1OFI3PTxxYU6=
LN229	M3nidmN6fG:2b4jpZ4l1gSCjc4PhfS=>		MnPoOFghcHK\|	NETqb4JEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNVjJ{OTDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG\|ZTDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTR2zszN	NIHwe\|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[1OFI3PSd-Mki2OVQzPjV:L3G+
NCI-H460	NIDFXI1EgXSxdH;4bYNqfHliYYPzZZk>		MlPYOFghcHK\|	MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDOR2kuUDR4MDDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG\|ZTDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTR5zszN	MmrrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4NUSyOlUoRjJ6NkW0NlY2RC:jPh?=
T47D	NVHnVJFYS3m2b4TvfIlkcXS7IHHzd4F6		MWO0PEBpenN?	NF34VGZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBVPDeGIHPlcIx{KGG\|c3Xzd4VlKGG\|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PDkQvF2=	NF24N409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[1OFI3PSd-Mki2OVQzPjV:L3G+
HepG2	NV\3PXBFTnWwY4Tpc44h[XO\|YYm=		NHX5cJoyKGi{	MnrUTY5pcWKrdHnvckBw\iCLTD22MWlv\HWlZXSgV3RCXDNiYXP0bZZifGmxbjCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBpfW2jbjDI[ZBIOiClZXzsd{BmgHC{ZYPzbY5oKHCVVFHUN{1tfWOrZnXyZZNmKHC{ZT3pcoN2[mG2ZXSg[o9zKDFiaIKgZoVnd3KnIFnMMVYhe3SrbYXsZZRqd25iZn;yJFYhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NF0zPC56zszN	MkfMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzNEC3NFUoRjJ7MUSwO\|A2RC:jPh?=
UOCB1	MX;BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NVv1SXAzPzJiaILz	M4XBO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVV;DRlEh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFPlcHRqfGW{LVfsc{Bie3OjeTygSWM2OD1\|NT62PVU5\|ryP	M1zVdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEC3PVc2Lz5{OUSwO\|k4PTxxYU6=
NB-EBc1	MWHxTHRUKGG\|c3H5			NYDSUoNYeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IF7CMWVD[zFiY3XscJM>	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
LNCAP	M17iUGFvfGmycn;sbYZmemG2aY\lJIF{e2G7			NHXwSFBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzOR2FRKGOnbHzzMEBKSzVyPUGw{txO	MkH0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2NU[xNVMoRjJ7NEW2NVE{RC:jPh?=
LNCAP	NGTXVVJCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		MV63NkBpenN?	M{nhUmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTF7DRXAh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UV{Bie3OjeTygTWM2OD1{NN88US=>	NUHZfmxnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0OVYyOTNpPkK5OFU3OTF\|PD;hQi=>
LNCAP	NXLNfIVXSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		Ml7rO\|IhcHK\|	NGfoOYRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzOR2FRKGOnbHzzJIFnfGW{IEeyJIhzeyCrbjDwdoV{\W6lZTDv[kBmdnqjbIX0ZY1q\GViYomgUXRUKGG\|c3H5MEBKSzVyPUOxMlfPxE1?	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR3NkGxN{c,Ojl2NU[xNVM9N2F-
LNCAP	NEfJelVHfW6ldHnvckBie3OjeR?=	MkD6Nk42KHWP	NYTEWm53OjRiaILz	MX;Jcohq[mm2aX;uJI9nKEiGQVO2M2hUWDlyIHnuJIh2dWGwIFzOR2FRKGOnbHzzJIF{e2W\|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKEGUIIDyc5RmcW5ibHX2[Ywh[XRiMj61JJVOKGGodHXyJFI1KGi{czDifUBY\XO2ZYLuJIJtd3RibXX0bI9l	MkHUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2NU[xNVMoRjJ7NEW2NVE{RC:jPh?=
BV2	MYLBcpRqcW6obHHtcYF1d3K7IHHzd4F6			M3vaXGFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgRnYzKGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gUHBUNWmwZIXj[YQhdmm2cnnjJI95cWSnIIDyc4R2[3Srb36sJGlEPTB;MkiuOO69VQ>?	MnzBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2OEK5OFAoRjJ7NEiyPVQxRC:jPh?=
MDA-MB-231/beta41	NVHCe4NlTXO2cn;n[Y5q[yCjc4PhfS=>		NHLablAyQCCqcoO=	MV;Fd5Rzd2enbnnjJIFkfGm4aYT5JIF1KEWUYnX0ZUApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCqdX3hckBOTEFvTVKtNlMyN2KndHG0NUBk\WyuczDh[pRmeiBzODDodpMh[nlicnXubYxt[SCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTB;MD6wNFI1\|ryP	M4nJVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkSxNlA3Lz5{OU[0NVIxPjxxYU6=
Ishikawa	NI\XXW1Ge3S{b3flcolkKGG\|c3H5		NHm1U3k6PiCqcoO=	NYjFb5dQTXO2cn;n[Y5q[yCjY4Tpeol1gSCjdDDFVoFteGijIHnuJIh2dWGwIFnzbIls[XejIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnu[JVkfGmxbjDv[kBidGujbHnu[UBxcG:\|cHjheIF{\SCjY4Tpeol1gSC3c3nu[{BxNW6rdILvdIhmdm:uIHHzJJN2[nO2cnH0[UB1emWjdHXkJIZweiB7NjDodpMh\m:ubH;3[YQh[nlic4Xid5Rz[XSnIHHk[Il1cW:wIHL5JJNx\WO2cn;wbI91d22ndILpZ{Bu\XSqb3SsJGVEPTB;MD6yOO69VQ>?	NVPDPVNkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OFEzODZpPkK5OlQyOjB4PD;hQi=>
HEK293	MXjGeY5kfGmxbjDhd5NigQ>?		Mor1OFghcHK\|	NFu5WZlC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGVTNWKndHGgeJJidnOoZXP0[YQhcW5iSFXLNlk{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4xOTl7Nd88US=>	NX;OVHI2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDRxJ{7DbGVOSkx:L3G+
HEK293	NHGwXIJHfW6ldHnvckBie3OjeR?=		MXK0PEBpenN?	M1nXNWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSXIu[WyyaHGgeJJidnOoZXP0[YQhcW5iSFXLNlk{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE42ODFzOd88US=>	MnLNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQwLz6FaFXNRmw9N2F-
HT-29	MnTCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?			MYTHdo94fGhiaX7obYJqfGmxbjDv[kBJd22xIIPhdIlmdnNiKHj1cYFvMSCKVD2yPUBk\WyuczDifUBOXFRiYYPzZZktKEeLPUSuNVHPxE1?	NYXoZnhiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDRxJ{7DbGVOSkx:L3G+
HL60	M{fPXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7			NIX4bZlIem:5dHigbY5pcWKrdHnvckBw\iCKb33vJJNieGmnboOgLIh2dWGwKTDIUFYxKGOnbHzzJIJ6KE2WVDDhd5NigSxiR1m9OE45Os7:TR?=	MoTzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQwLz6FaFXNRmw9N2F-
SGC7901	Moq5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?			M3ruXWdzd3e2aDDpcohq[mm2aX;uJI9nKEixbX:gd4FxcWWwczCobJVu[W5rIGPHR\|c6ODFiY3XscJMh[nliTWTUJIF{e2G7LDDHTV02Njd6zszN	M2PBOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1Nyd-Q3jFUWJNRC:jPh?=
HL60	NXrOZYRVTnWwY4Tpc44h[XO\|YYm=			M3TVVGNwdXCndHn0bZZmKGmwaHnibZRqd25ib3[gS2xWXDFiaX6gbJVu[W5iSFy2NEBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iMj3bNUwzNTOKXXTlc5h6NURvZ3z1Z49{\SC3cIThb4UhdWWjc4Xy[YQh\m:{IEOwJJNm[3NiYomg[I92[mynIILlZ4lxem:lYXygdIxwfCCjbnHsfZNqeyxiS3m9O:69VQ>?	M37Lc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1Nyd-Q3jFUWJNRC:jPh?=
CHO	M2HUZWZ2dmO2aX;uJIF{e2G7			MmTmR49ueGW2aYTpeoUhcW6qaXLpeIlwdiCxZjDHUHVVOSBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwIEKtX\|EtOi1\|SG3k[Y95gS2GLXfseYNwe2VidYD0ZYtmKG2nYYP1doVlKG[xcjCzNEB{\WO\|IHL5JIRwfWKuZTDy[YNqeHKxY3HsJJBtd3RiYX7hcJl{cXNuIFvpQVfPxE1?	NH;4Nng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PC9pPlPoSW1DVDxxYU6=
3T3L1	Ml7rSpVv[3Srb36gZZN{[Xl?			NVS2cXlbUW6qaXLpeIlwdiCxZjDHUHVVPCCrbjDtc5V{\SB\|VEPMNUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iaX7zeYxqdi2\|dHnteYxifGWmIEKt[IVwgHlvRD3bNVREZWeudXPvd4UhfXC2YXvlMEBKSzVyPUKw{txO	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OE8oRkOqRV3CUFww[T5?

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	DNMT-1 / DNMT-3a / DNMT-3b ; PubMed: 25931004 J Steroid Biochem Mol Biol Western blot analysis of the effect of physiological concentrations of genistein (0.5–10 µmol/L) on protein expression of the three DNMT subtypes, DNMT1, DNMT3a and DNMT3b in PCa cells. p-AKT / AKT ; PubMed: 27574003 Int J Oncol Genistein inhibits Akt phosphorylation. MCF-7-C3 and T47D cells were treated with genistein at indicated concentrations for 16 h. Phospho-Akt and Akt were detected with western blotting. *P<0.05. CIP2A / PARP / Cleaved PARP / Caspase-3 / Cleaved Caspase-3 ; PubMed: 27574003 Int J Oncol MCF-7, MCF-7-C3 and T47D cells were treated with genistein (GENI) at indicated concentrations for 48 h. Protein levels of CIP2A and PARP in all three cell lines, and caspase-3, cleaved caspase-3 (C-caspase-3) in MCF-7-C3 and T47D cells were detected with western blot analysis. Foxo3; PubMed: 21639915 BMC Cancer Genistein inhibits EGF-induced translocation of FOXO3. Immunofluorescent staining of experimental monolayers revealed that EGF-induced FOXO3 translocation from the nucleus to the cytosol was inhibited by genistein. This experiment was repeated two times in triplicate. p-ERK; PubMed: 18384126 J Cell Biochem Genistein dose-dependently inhibits the phosphorylation of ERK1/2. Cells were treated as above and harvested at day 5 after 5 min exposure to adipogenic cocktail, and whole cell protein were subjected to western blotting analysis. PPARγ / C/EBPα / αP2 ; PubMed: 18384126 J Cell Biochem Genistein dose-dependently inhibits adipogenic gene expression. Mouse BMSC cultures were exposed to adipogenic cocktail in the presence of increasing concentrations of genistein. Cells were harvested at day 21, and whole cell proteins were subjected to western blotting analysis.	25931004 27574003 21639915 18384126
Immunofluorescence	ER-β / pS87 ER-β ; PubMed: 25931004 J Steroid Biochem Mol Biol Cells were treated with DMSO (vehicle) or genistein 1 µmol/L and 10 µmol/L for 4hr. Then, cells were fixed, immunostained with pan ER-β and pS87 antibodies simultaneously and visualized by the confocal microscope. The red fluorescent staining in the first column is for pan ER-β, the green fluorescent staining in the second column is for pS87 and the third column is for both images merged together using image J software.	25931004

In vivo

Genistein has chemopreventive effects on breast, prostate, and other endocrine-dependent tumors in adult animals. Genistein in the diet reduced the incidence of poorly differentiated prostatic adenocarcinomas in a dose-dependent manner and down-regulated androgen receptor, estrogen receptor-alpha, progesterone receptor, epidermal growth factor receptor, insulin-like growth factor-I, and extracellular signal-regulated kinase-1 but not estrogen receptor-beta and transforming growth factor-alpha mRNA expressions. Dietary genistein protects against mammary and prostate cancers by regulating specific sex steroid receptors and growth factor signaling pathways.^[4] Genistein combined with prostate tumor irradiation causes greater inhibition of primary tumor growth and increases control of spontaneous metastasis to para-aortic lymph nodes, increasing mouse survival. Paradoxically, treatment with genistein alone increases metastasis to lymph nodes. ^[5]

Protocol

References

- Collapse

Solubility (25°C)

In vitro	DMSO	54 mg/mL (199.82 mM)
	Water	Insoluble
	Ethanol	''2 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Genistein (NPI 031L) SDF

Molecular Weight	270.24
Formula	C₁₅H₁₀O₅
CAS No.	446-72-0
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	C1=CC(=CC=C1C2=COC3=CC(=CC(=C3C2=O)O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT01982578	Unknown status	Dietary Supplement: Genistein\|Other: Placebo	Alzheimer''s Disease	Fundación para la Investigación del Hospital Clínico de Valencia\|University of Valencia	September 1 2017	Not Applicable
NCT02499861	Completed	Drug: Decitabine and Genistein	Cancer	St. Justine''s Hospital	July 2015	Phase 1\|Phase 2
NCT01628471	Completed	Drug: decitabine in combination with genistein	Non Small Cell Lung Cancer	Uman Pharma\|DSM Nutritional Products Inc.\|MDEIE Ministry Québec Government\|INRS-Institut Armand Frappier Université du Québec	November 2012	Phase 1\|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

EGFR Signaling Pathway Map

Related EGFR Products

Erlotinib (OSI-774) ^New

Erlotinib (OSI-774, CP358774, NSC 718781, Tarceva) is an EGFR inhibitor with IC50 of 2 nM, >1000-fold more sensitive for EGFR than human c-Src or v-Abl. Erlotinib induces autophagy.
Afatinib (BIBW2992) Dimaleate ^New

Afatinib (BIBW2992) Dimaleate irreversibly inhibits EGFR/HER2 including EGFR(wt), EGFR(L858R), EGFR(L858R/T790M) and HER2 with IC50 of 0.5 nM, 0.4 nM, 10 nM and 14 nM, respectively; 100-fold more active against Gefitinib-resistant L858R-T790M EGFR mutant. Afatinib (BIBW2992) Dimaleate induces autophagy.
Poziotinib (HM781-36B) ^New

Poziotinib (HM781-36B, NOV120101) is an irreversible pan-HER inhibitor with IC50 of 3.2 nM, 5.3 nM and 23.5 nM for HER1, HER2, and HER4, respectively. Poziotinib also induces apoptosis and G1 cell cycle arrest. Phase 2.
Gefitinib (ZD1839)

Gefitinib (ZD-1839, Iressa) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively. Gefitinib promotes autophagy and apoptosis of lung cancer cells via blockade of the PI3K/AKT/mTOR pathway.

Features:A potent EGFR tyrosine kinase inhibitor.
Erlotinib HCl (OSI-744)

Erlotinib HCl (OSI-744, CP358774, NSC 718781) is an EGFR inhibitor with IC50 of 2 nM in cell-free assays, >1000-fold more sensitive for EGFR than human c-Src or v-Abl.
Afatinib (BIBW2992)

Afatinib (BIBW2992) inhibits EGFR/ErbB irreversibly in vitro with IC50 of 0.5, 0.4, 10, 14, 1 nM for EGFR^wt, EGFR^L858R, EGFR^L858R/T790M ErbB2 (HER2) and ErbB4 (HER4), respectively. Afatinib induces autophagy.
Osimertinib (AZD9291)

Osimertinib (AZD9291) is an oral, irreversible, and mutant-selective EGFR inhibitor with IC50 of 12.92, 11.44 and 493.8 nM for Exon 19 deletion EGFR, L858R/T790M EGFR, and WT EGFR in LoVo cells, respectively. Phase 3.
Lapatinib (GW-572016)

Lapatinib (GW-572016, GSK572016, GW2016), used in the form of Lapatinib Ditosylate, is a potent EGFR and ErbB2 inhibitor with IC50 of 10.8 and 9.2 nM in cell-free assays, respectively. Lapatinib induces ferroptosis and autophagic cell death.
AG-490 (Tyrphostin B42)

AG-490 (Tyrphostin B42, Zinc02557947) is an inhibitor of EGFR with IC50 of 0.1 μM in cell-free assays, 135-fold more selective for EGFR versus ErbB2, also inhibits JAK2 with no activity to Lck, Lyn, Btk, Syk and Src.
Rociletinib (CO-1686)

Rociletinib (CO-1686, AVL-301) is an irreversible, mutant-selective EGFR inhibitor with K_i of 21.5 nM and 303.3 nM for EGFR^L858R/T790M and EGFR^WT in cell-free assays, respectively. Phase 2.

Choose Your Country or Region

By Signaling Pathways

By product type

Genistein (NPI 031L)