Genistein (NPI 031L)

Catalog No.S1342 Synonyms: NPI 031L

For research use only.

Genistein (NPI 031L), a phytoestrogen found in soy products, is a highly specific inhibitor of protein tyrosine kinase (PTK) which blocks the mitogenic effect mediated by EGF on NIH-3T3 cells with IC50 of 12μM or by insulin with IC50 of 19 μM.

Genistein (NPI 031L) Chemical Structure

CAS No. 446-72-0

Selleck's Genistein (NPI 031L) has been cited by 26 publications

Purity & Quality Control

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Biological Activity

Description Genistein (NPI 031L), a phytoestrogen found in soy products, is a highly specific inhibitor of protein tyrosine kinase (PTK) which blocks the mitogenic effect mediated by EGF on NIH-3T3 cells with IC50 of 12μM or by insulin with IC50 of 19 μM.
Targets
EGFR [1] topo II [1]
In vitro

Genistein is an ATP competitive inhibitor. Genistein inhibits tyrosine phosphorylation in isolated enzyme and receptor preparations and in whole cells including platelets, lymphocytes and a variety of cultured cells. It also inhibits EGF-stimulated phosphorylation in cultured cells as well as inhibition of Topo II (topoisomerase II). Genistein inhibits EGF-stimulated tyrosine phosphorylation in cultured A431 epidermoid carcinoma cells. Inhibition is competitive with ATP and noncompetitive with substrate. [1] Genistein blocks the mitogenic effect mediated by EGF, insulin and thrombin on NIH-3T3 cells.[2] Genistein also acts as an agonist at the GPR30 receptor and binds to PPARγ and estrogen receptors. Genistein also binds to PPARγ, acting as an agonist at this receptor with Ki of 5.7 μM. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BxPC3 MlrHVJJwdGmoZYLheIlwdiCjc4PhfS=> M1LRSGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQojQR|Mh[2WubDDsbY5mKGK7IF3UWEBie3OjeTygTWM2OD1|MDFOwG0> MkGzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ5OEm3N|coRjF4N{i5O|M4RC:jPh?=
BT20 MWDQdo9tcW[ncnH0bY9vKGG|c3H5 MWTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEKWMkCgZ4VtdCCuaX7lJIJ6KE2WVDDhd5NigSxiSVO1NF01PiEQvF2= MoLPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ5OEm3N|coRjF4N{i5O|M4RC:jPh?=
ANN-1 M4LIfGN6fG:2b4jpZ{Bie3OjeR?= NGnVdGlEgXSxdH;4bYMh\W[oZXP0JI9vKHZvYXLsJJRz[W6|Zn;ycYVlKG23cnnu[UBCVk5vMTDj[YxteyxiSVO1NF05|ryP MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85OjBzNkCzK|45OjBzNkCzQE9iRg>?
breast carcinoma cells MlnMR5l1d3SxeHnjJIF{e2G7 NHnPbGxEgXSxdH;4bYMh\W[oZXP0JI9vKE2FRj23JIh2dWGwIHLy[YF{fCClYYLjbY5wdWFiY3XscJMtKEmFNUC9NVUvOc7:TR?= Mn3RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDJyMU[wN{c,QDJyMU[wN|ww[T5?
3T3 MoDzR5l1d3SxeHnjJIF{e2G7 M4jse2N6fG:2b4jpZ{Bm\m[nY4Sgc44hO1R|IHPlcIx{NCCLQ{WwQVI1|ryP MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85OjBzNkCzK|45OjBzNkCzQE9iRg>?
colon cells MUXDfZRwfG:6aXOgZZN{[Xl? Mn23R5l1d3SxeHnjJIVn\mWldDDvckBYcUS{IHj1cYFvKGOxbH;uJINmdGy|LDDJR|UxRTJ5LkhOwG0> NFTVcJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96MkCxOlA{Lz56MkCxOlA{RC:jPh?=
Hepa1clc7 NXrjVlVXS3m2b4TvfIlkcXS7IHHzd4F6 M1njN|czKGi{cx?= MmnsR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgTIVx[TGlbHO3JINmdGy|IHHmeIVzKDd{IHjyd{whUUN3ME2xNe69VQ>? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB5NUe0Nkc,OTByN{W3OFI9N2F-
MCF7 NYLmd4tNTnWwY4Tpc44h[XO|YYm= M4PsblYhcHK| MoTWTY5pcWKrdHnvckBw\iCyaH;yZo9tKGW|dHXyMYlv\HWlZXSgc5JvcXSqaX7lJIRm[2G{Yn;4fYxie2ViaX6gbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB4IHjyd{whUUN3ME2yOu69VQ>? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB5NUe0Nkc,OTByN{W3OFI9N2F-
3T3/A31 NHXxWW5EgXSxdH;4bYNqfHliYYPzZZk> MnXNNUB1dyBzMECgeYcwdUx? MVu3NkBpenN? NW\yV|VRS3m2b4TvfIlkcXS7IHHnZYlve3RiQlHMRk9kKG2xdYPlJINtd26nZDCzWFMwSTNzIHPlcIx{KGG2IEGgeI8hOTByIIXnM41NKGGodHXyJFczKGi{czDifUBvcWe{b4PpckBie3OjeR?= MmLhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByOU[4OlMoRjFyMEm2PFY{RC:jPh?=
SH-SY5Y NYXMcm5XTnWwY4Tpc44h[XO|YYm= NH\OdHBC\2:waYP0JIFkfGm4aYT5JIlvKHS{YX7zZ5JqeHSrb37hcEBi[3SrdnH0bY9vKGG|c3H5JIlvKFOKLWPZOXkhdmW3cn;icIF{fG:vYTDj[YxteyCneIDy[ZN{cW6pIFXzeJJw\2WwIILlZ4VxfG:{IHLleIEtKEWFNUC9NE4xODF5zszN Mn7VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF7ME[yPFAoRjFzOUC2NlgxRC:jPh?=
HeLa NFLnTIFHfW6ldHnvckBie3OjeR?= MXLBZ5RqfmG2aX;uJI9nKGW|dILv[4VvKHKnc4DvcpNmKGWuZX3lcpQhcW5iSHXMZUBk\WyuczDzeIFjdHlidILhcpNn\WO2ZXSge4l1cCCqdX3hckBGe3S{b3flckBz\WOncITvdkBj\XSjLjygSWM2OD1yLkCwOFHPxE1? NHjNU4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUmwOlI5OCd-MUG5NFYzQDB:L3G+
HeLa NX3hOoJSTnWwY4Tpc44h[XO|YYm= NIrlenpC[3SrdnH0bY9vKG:oIHXzeJJw\2WwIILld5BwdnOnIHXs[Y1mdnRiaX6gTIVN[SClZXzsd{B{fGGkbImgeJJidnOoZXP0[YQhf2m2aDDoeY1idiCHc4Tyc4dmdiC{ZXPldJRweiCjbIDoZU4tKEWFNUC9NE4xPDkQvF2= Ml7zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF7ME[yPFAoRjFzOUC2NlgxRC:jPh?=
Ishikawa MoruSZN1em:pZX7pZ{Bie3OjeR?= NXzHZXF4PCCmYYnz MmDsSZN1em:pZX7pZ{Bi[3Srdnn0fUBqdiCqdX3hckBKe2ira3H3ZUBk\WyuczDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZiYXzrZYxqdmVicHjvd5Bp[XSjc3WgZYN1cX[rdImgZYZ1\XJiNDDkZZl{KGK7IIDhdoEudmm2cn;wbIVvd2xicnXs[YF{\SCjc4PhfUwhUUN3ME2wMlUy|ryP NI\zO289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkWwNlMxPyd-MUK1NFI{ODd:L3G+
Hepa-1c1c7 NVPwUoU5TnWwY4Tpc44h[XO|YYm= MlTOTY5lfWO2aX;uJI9nKE6DRGDIPpF2cW6xbnWgdoVlfWO2YYPlJIlvKG2xdYPlJGhmeGFvMXOxZ|ch[2WubIOgZZN{\XO|ZXSgZZMh\HK3ZzDs[ZZmdCC{ZYH1bZJm\CC2bzDkc5VjdGViZX76fY1mKGGldHn2bZR6KGK7IF3UWEBie3OjeTygR2Q:OjJwOd88US=> MkSwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR3MUC1PVAoRjF2NUGwOVkxRC:jPh?=
Hepa-1c1c7 M2nOcWZ2dmO2aX;uJIF{e2G7 Mk\0TY5pcWKrdHnvckBw\iCvb4Xz[UBJ\XCjLUHjNYM4KGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NF01PS5{zszN NUHkS4xTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS1NVA2QTBpPkG0OVExPTlyPD;hQi=>
T47D NEfxVIhGe3S{b3flcolkKGG|c3H5 M3;BXWV{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hXDR5RDDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegd5RqdXWuYYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:wIHXxeYl3[WynboSgeI8hOTBicF2g[ZN1emGmaX;sJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gT1yLkCwNe69VQ>? M{DOZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{i3OFM3Lz5zNUe4O|Q{PjxxYU6=
MCF7 M2j3XWV{fHKxZ3XubYMh[XO|YYm= M{TTemV{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegd5RqdXWuYYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:wIHXxeYl3[WynboSgeI8hOTBicF2g[ZN1emGmaX;sJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gT1yLkCwN:69VQ>? M4\pPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{i3OFM3Lz5zNUe4O|Q{PjxxYU6=
T47D NXjKZoNiTXO2cn;n[Y5q[yCjc4PhfS=> NEXafIFGe3S{b3flcolkKGGldHn2bZR6KGmwIHj1cYFvKFR2N1SgZ4VtdHNiYYPz[ZN{\WRiYYOg[JJ2\yCuZY\lcEBk[XW|aX7nJJN1cW23bHH0bY9vKG:oIHPlcIwheHKxbHnm[ZJifGmxbjDldZVqfmGuZX70JJRwKDFyMDDwUUBme3S{YXTpc4wh[nliYXzhcYFzKGKudXWgZZN{[XluIFHjeIl3cXS7PUCuNFA6|ryP NWX5RYVpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3PFc1OzZpPkG1O|g4PDN4PD;hQi=>
MCF7 NX6ySZR[TXO2cn;n[Y5q[yCjc4PhfS=> MXfFd5Rzd2enbnnjJIFkfGm4aYT5JIlvKGi3bXHuJG1ETjdiY3XscJMh[XO|ZYPz[YQh[XNiZIL1[{Bt\X[nbDDjZZV{cW6pIIP0bY12dGG2aX;uJI9nKGOnbHygdJJwdGmoZYLheIlwdiCncYXpeoFt\W62IITvJFExOCCyTTDld5Rz[WSrb3ygZpkh[WyjbXHyJIJtfWViYYPzZZktKEGldHn2bZR6RTBwMEGz{txO MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd6N{SzOkc,OTV5OEe0N|Y9N2F-
293T M4jCeWZ2dmO2aX;uJIF{e2G7 MorWSIl{eGyjY3Xt[Y51KG:oIGuzTH0yP2KndHGt[ZN1emGmaX;sJIZzd21icnXjc41jcW6jboSgbJVu[W5iRWLi[ZRiKGW6cILld5Nm\CCrbjCyPVNVKGOnbHzzMEBKSzVyPUCuNFAyO87:TR?= M1nRcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4MkG5OFY{Lz5zNkKxPVQ3OzxxYU6=
293T Mn7aSpVv[3Srb36gZZN{[Xl? M{XtSmRqe3CuYXPlcYVvfCCxZjDbN2heOTekZYThMYV{fHKjZHnvcEBnem:vIILlZ49u[mmwYX70JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJFI6O1RiY3XscJMtKEmFNUC9NE4xOjl2zszN MnvuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ{MUm0OlMoRjF4MkG5OFY{RC:jPh?=
Vero NY\OU3NYS3m2b4TvfIlkcXS7IHHzd4F6 NFmwRVdEgXSxdH;4bYNqfHliYXfhbY5{fCCjZoLpZ4FvKGe{ZXXuJI1wdmuneTDW[ZJwKGOnbHzzMEBKSzVyPUOyMlnPxE1? NEXteoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkS0NVA3Pid-MU[0OFExPjZ:L3G+
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RAW 264 NX65ZnVNTnWwY4Tpc44h[XO|YYm= MVKyOEBpenN? M3;MUGlvcGmkaYTpc44hd2ZiMTD1[{9udCCOUGOtd5RqdXWuYYTl[EBVVk[jbIDoZUBi[2O3bYXsZZRqd25iaX6gVmFYKDJ4NDDj[YxteyCjZoTldkAzPCCqcoOsJGlEPTB;MUiuNe69VQ>? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN{MEK0Okc,OTd|MkCyOFY9N2F-
K562 NF2wZVVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml;IOUBl[Xm| NUPvVXlKT3Kxd4ToJIlvcGmkaYTpc44hd2ZiS{W2NkBk\WyuczDifUBZXFRiYYPzZZkh[W[2ZYKgOUBl[Xm|LDDJR|UxRTF5LkW2{txO NVvqZVV2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVExQTJpPkG3OFEyODl{PD;hQi=>
HUVE12 MonlSpVv[3Srb36gZZN{[Xl? MlvPVoV3\XK|YXygc4YhcHmmcn;n[Y4heGW{b4jp[IUucW6mdXPl[EBNTEhiYXP0bZZqfHliaX6gbJVu[W5iSGXWSVEzKGOnbHzz NVPJTIxHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiwOlg6QDBpPkG4NFY5QThyPD;hQi=>
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L1210 MXzBcpRqdGW3a3XtbYMh[XO|YYm= MmrXNlQhcHK| NYCxN4xiSW62aXzleYtmdWmlIHHjeIl3cXS7IHHnZYlve3RibX;1d4UhVDF{MUCgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KGOub37v[4VvcWNiYYPzZZktKEyFNUC9Ok42|ryP MoPBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThzNkO1PFkoRjF6MU[zOVg6RC:jPh?=
MOLT3 MlTHRY51cWyndXvlcYlkKGG|c3H5 MmC1NlQhcHK| NH\FTYNCdnSrbHX1b4VucWNiYXP0bZZqfHliYXfhbY5{fCCqdX3hckBOV0yWMzDj[YxteyCjZoTldkAzPCCqcoOgZpkh[2yxbn;n[Y5q[yCjc4PhfUwhVEN3ME23MlXPxE1? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDF4M{W4PUc,OThzNkO1PFk9N2F-
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Raji MXHBcpRqdGW3a3XtbYMh[XO|YYm= NVrDcotPOjRiaILz MnjqRY51cWyndXvlcYlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gVoFrcSClZXzsd{Bi\nSncjCyOEBpenNiYomgZ4xwdm:pZX7pZ{Bie3OjeTygUGM2OD1zMz63{txO Mn3rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThzNkO1PFkoRjF6MU[zOVg6RC:jPh?=
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L1210 NH3Qc|NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M176flI1KGi{cx?= MV;Hdo94fGhiaX7obYJqfGmxbjDv[kBud3W|ZTDMNVIyOCClZXzsd{Bi\nSncjCyOEBpenNuIFnHOVA:OThwNd88US=> NXz1dZNPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUixOlM2QDlpPkG4NVY{PTh7PD;hQi=>
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MCF-7 MX NYfGTHF5TnWwY4Tpc44h[XO|YYm= NXL3bYpRUW6qaXLpeIlwdiCxZjDCR3JRKGW6cILld5Nm\CCrbjDNR2YuPyCPWDDj[YxteyC3c3nu[{BJd2WlaIP0JFM{OzR{IIP0ZYlvcW6pLDDJR|UxRThwON88US=> Mo\qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NUS4NFAoRjJzM{W0PFAxRC:jPh?=
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NCI-ADR-RES NEPlXW9CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NUXRWJhlPDhiaILz MXnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCQQ1mtRWRTNVKHUzDj[YxteyCrbnP1ZoF1\WRid3n0bEAyODBibl2gc4Yh\XO2cnHkbY9tKGGwZDCwMlA4KG6PIH;mJJRme3Sxc4Tldo9v\SCjZoTldkA1QCCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTDpckBxemW|ZX7j[UBw\iCoZYThcEBjd3[rbnWgd4VzfW1iYX7kJG52W2W{dX2sJGdKPTB;Mk[uPVE2PM7:TR?= M4PIeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlc2Lz5{MUWxN|I4PTxxYU6=
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NCI-ADR-RES MUXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M2TwdFQ5KGi{cx?= MlrwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDld5Rzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iTlPJMWFFWi2URWOgZ4VtdHNiaX7jeYJifGWmIIfpeIghOC5yMEOgcm0hd2ZiZYP0doFlcW:uIHHu[EAxNjBzIH7NJI9nKHSnc4Tvd5Rmem:wZTDh[pRmeiB2ODDodpMh[nlic4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUBqdiCycnXz[Y5k\SCxZjC1KUBn\XSjbDDic5ZqdmVic3XyeY0tKEeLNUC9N|IvOzV7NN88US=> NVP0TIZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzPzVpPkKxOVE{Ojd3PD;hQi=>
MCF12A NYDsc4RZSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MUi0PEBpenN? MmCxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDld5Rzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iTVPGNVJCKGOnbHzzJIlv[3WkYYTl[EB4cXSqIECuNFA{KG6PIH;mJIV{fHKjZHnvcEBidmRiMD6wNUBvVSCxZjD0[ZN1d3O2ZYLvcoUh[W[2ZYKgOFghcHK|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XliaX6gdJJme2WwY3Wgc4YhPSViZnX0ZYwh[m:4aX7lJJNmenWvLDDHTVUxRTN{LkO1PVTPxE1? NYX0Z|BtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzPzVpPkKxOVE{Ojd3PD;hQi=>
LNCAP NVLSNGV2SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MWC0PEBpenN? NHXXNYFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzOR2FRKGOnbHzzJIV5eHKnc4Ppcoch[W6mcn;n[Y4hemWlZYD0c5IhcW6ldXLheIVlKHerdHigNE43KG6PIH;mJIV{fHKjZHnvcEBidmRiMD63JI5OKG:oIITld5Rwe3Sncn;u[UBi\nSncjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiR1m1NF0{QC57MES1{txO NGHJdmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxN|I4PSd-MkG1NVMzPzV:L3G+
PC3 M17yTWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MoHwOFghcHK| MmXjRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDhcoRzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iUFOzJINmdGy|IHnuZ5Vj[XSnZDD3bZRpKDBwNjDuUUBw\iCnc4TyZYRqd2xiYX7kJFAvPyCwTTDv[kB1\XO2b4P0[ZJwdmViYX\0[ZIhPDhiaILzJIJ6KHO3bH\vdohw\GGvaX7lJGIh[XO|YYmsJGdKPTB;NEKuOlU4Qc7:TR?= MnzQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUOyO|UoRjJzNUGzNlc2RC:jPh?=
MCF7 NUTU[FFiSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M{f2OFQ5KGi{cx?= MXXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiZYjwdoV{e2mwZzDld5Rzd2enbjDy[YNmeHSxcjDpcoN2[mG2ZXSge4l1cCByLkC2JI5OKG:oIHXzeJJi\GmxbDDhcoQhOC5yNzDuUUBw\iC2ZYP0c5N1\XKxbnWgZYZ1\XJiNEigbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJiYYPzZZkhcW5icILld4Vv[2Vib3[g[oV1[WxiYn;2bY5mKHOncoXtJIFv\CCQdWPldpVuNCCJSUWwQVQ2NjdyOElOwG0> M1PqSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlc2Lz5{MUWxN|I4PTxxYU6=
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MCF7 MofvRY51cWW|dILv[4VvcWNiYYPzZZk> MXqxJJRwKDJyIIXN MkLBOFghcHK| MXLBcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCHUj3wc5NqfGm4ZTDNR2Y4KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gNVdj\XSjIHXzeJJi\GmxbD3pcoR2[2WmIIPlZ5JmfGWmIHHsb4FtcW6nIIDoc5NxcGG2YYPlJIFkfGm4aYT5JIF1KDFidH:gNlAhfU1iYX\0[ZIhPDhiaILzJIJ6KHCqb4PwbIEudGmpaISgdoVxd3K2ZYKgZ4hmdWmudX3pcoV{[2WwY3WgZZN{[Xl? Ml3nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6MEC4OVkoRjJzOECwPFU6RC:jPh?=
Jurkat T MmPVRZBweHSxc3nzJIF{e2G7 NG\xN2Y{PiCqcoO= NULuS40xUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDKeZJs[XRiVDDj[YxteyCxdnXy[ZhxemW|c3nu[{BP\W9iYX\0[ZIhOzZiaILzJIJ6KG[ub4egZ5l1d22ndIL5 NWHacG5oRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxPVc{QTNpPkKyNVk4Ozl|PD;hQi=>
FRT NGDpN49HfW6ldHnvckBie3OjeR?= MVjQc5RmdnSrYYTpc44h[WO2aY\peJkh[XRiaIXtZY4hS0[WUjDk[Yx1[SCINUC4JI12fGGwdD3t[YRq[XSnZDDpc4RqdmViZnz1fEBmgHC{ZYPz[YQhcW5icnH0JGZTXCClZXzsd{Bkd2W6cILld5NqdmdiZnz1c5Jme2OnboSgdJJwfGWrbjDZSnAuUDF2OGGvNVE2OkxiYomg[ox2d3Knc3PlcoNmKGG|c3H5MEBGSzVyPUhOwG0> MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ6MUG4OUc,OjJ{OEGxPFU9N2F-
Human Huh7.5.1 MYPBcpRqfmm{YXygZZN{[Xl? NGTNWGc4OiCqcoO= NYLPc25ISW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEOYIFrGTE0yKEp|OUnFUUBqdm[nY4Tl[EBqdiCKdX3hckBJfWh5LkWuNUBk\WyuczDhd5Nme3OnZDDhd{B{fXCycnXzd4lwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDh[pRmeiB5MjDodpMh[nliRVfGVEBie3OjeTygSWM2OD1zND60{txO M1\iZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NES1N|I5Lz5{MkS0OVMzQDxxYU6=
CHO MWjGeY5kfGmxbjDhd5NigQ>? Mle2T4khfmGudXXzJIZweiC|b3TpeY0h\my3b4Lld4NmcW5iKEGwJJVOMSC3cIThb4UhcW5iT1HUVFFDOy22cnHud4Zm[3SnZDDDTG8h[2WubIOsJGtqRTJwOUdOwG0> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV5MUSxOUc,OjN3N{G0NVU9N2F-
CHO NX;qSnU6TnWwY4Tpc44h[XO|YYm= NVj5fFBVeEmFNUCgeoFtfWW|IH\vdkB{d2SrdX2g[ox2d3Knc3PlbY4hMDFyIIXNLUB2eHSja3WgbY4hV0GWUEHCN{11emGwc3\lZ5Rm\CCFSF:gZ4VtdHNuIFnDOVA:Oy56OUC0Oe69VQ>? MkLXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3N{G0NVUoRjJ|NUexOFE2RC:jPh?=
CHO NWD4SJBqTnWwY4Tpc44h[XO|YYm= NUXYeWREU2lidnHseYV{KG[xcjDzc4RqfW1iZnz1c5Jme2OnaX6gLFExKHWPKTD1dJRic2ViaX6gU2FVWDGEMT30doFve2[nY4Tl[EBEUE9iY3XscJMtKEurPUZOwG0> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV5MUSxOUc,OjN3N{G0NVU9N2F-
CHO NWTqNItzTnWwY4Tpc44h[XO|YYm= M4L5[5BKSzVyII\hcJVmeyCob4Kgd49lcXWvIH\seY9z\XOlZXnuJEgyOCC3TTmgeZB1[WunIHnuJG9CXFBzQkGteJJidnOoZXP0[YQhS0iRIHPlcIx{NCCLQ{WwQVkvOTJyMUJOwG0> M1zHSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUexOFE2Lz5{M{W3NVQyPTxxYU6=
HuH7 MYjBcpRqeGyjc33v[IlidCCjc4PhfS=> NXv6bm5tOjRiaILz M1PZd2FvfGmybHHzcY9lcWGuIHHjeIl3cXS7IHHnZYlve3RibHn2[ZIhe3Cxcn;6c4l1\SC|dHHn[UBw\iCSbHHzcY9lcXWvIHLldodp\WliQV7LRUBmgHC{ZYPzbY5oKEeIUDDpcoZm[3SnZDDpckBpfW2jbjDIeWg4KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gdIFz[XOrdHWg[IV3\WyxcH3lcpQh[W[2ZYKgNlQhcHK|IHL5JJFTXC2SQ2KgZY5idHm|aYOsJGlEPTB;MkFOwG0> NGLUbYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGyOVg1QSd-MkSxNlU5PDl:L3G+
SKBR3 MYXD[YxtKGO7Y3zlJIF{e2G7 NYP0XoczPTBidH:gO|UhfU1? MnrtN|YhcHK| MkHQR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gV2tDWjNiY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6gZYNkfW23bHH0bY9vKGG2IFewM2cyKHCqYYPlJIF1KDVyIITvJFc2KHWPIHHmeIVzKDN4IHjyd{BjgSCycn;wbYRqfW1iaX;kbYRmKHO2YXnubY5oNWKjc3XkJIZtd3diY4n0c41mfHKrYzDhcoFtgXOrcx?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR3NkCwOEc,OjR2NU[wNFQ9N2F-
A431 MW\D[YxtKGO7Y3zlJIF{e2G7 MmfYOVAhfG9iN{WgeW0> NWLzeG5sOzZiaILz MUPD[YxtKGO7Y3zlJIFzemW|dDDpckBpfW2jbjDBOFMyKGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIHHjZ5VufWyjdHnvckBifCCJMD;HNUBxcGG|ZTDheEA2OCC2bzC3OUB2VSCjZoTldkA{PiCqcoOgZpkheHKxcHnkbZVuKGmxZHnk[UB{fGGrbnnu[{1j[XOnZDDmcI94KGO7dH;t[ZRzcWNiYX7hcJl{cXN? M2[5fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEW2NFA1Lz5{NES1OlAxPDxxYU6=
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Assay
Methods Test Index PMID
Western blot PPARγ / C/EBPα / αP2 ; p-ERK ; Foxo3 ; CIP2A / PARP / Cleaved PARP / Caspase-3 / Cleaved Caspase-3 ; p-AKT / AKT ; DNMT-1 / DNMT-3a / DNMT-3b 18384126 21639915 27574003 25931004
Immunofluorescence ER-β / pS87 ER-β 25931004
In vivo Genistein has chemopreventive effects on breast, prostate, and other endocrine-dependent tumors in adult animals. Genistein in the diet reduced the incidence of poorly differentiated prostatic adenocarcinomas in a dose-dependent manner and down-regulated androgen receptor, estrogen receptor-alpha, progesterone receptor, epidermal growth factor receptor, insulin-like growth factor-I, and extracellular signal-regulated kinase-1 but not estrogen receptor-beta and transforming growth factor-alpha mRNA expressions. Dietary genistein protects against mammary and prostate cancers by regulating specific sex steroid receptors and growth factor signaling pathways.[4] Genistein combined with prostate tumor irradiation causes greater inhibition of primary tumor growth and increases control of spontaneous metastasis to para-aortic lymph nodes, increasing mouse survival. Paradoxically, treatment with genistein alone increases metastasis to lymph nodes. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 270.24
Formula

C15H10O5

CAS No. 446-72-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CC=C1C2=COC3=CC(=CC(=C3C2=O)O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05073523 Recruiting Other: Diet Healthy Chalmers University of Technology September 27 2021 Not Applicable
NCT01982578 Completed Dietary Supplement: Genistein|Other: Placebo Alzheimer''s Disease Fundación para la Investigación del Hospital Clínico de Valencia|University of Valencia September 1 2017 Not Applicable
NCT02499861 Completed Drug: Decitabine and Genistein Cancer St. Justine''s Hospital July 2015 Phase 1|Phase 2
NCT01628471 Completed Drug: decitabine in combination with genistein Non Small Cell Lung Cancer Uman Pharma|DSM Nutritional Products Inc.|MDEIE Ministry Québec Government|INRS-Institut Armand Frappier Université du Québec November 2012 Phase 1|Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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