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Genistein (NPI 031L)

Name: Genistein (NPI 031L)
Brand: Selleck Chemicals
SKU: S1342
Rating: 5 (26 reviews)

Catalog No.S1342 Synonyms: NPI 031L

For research use only.

Genistein (NPI 031L), a phytoestrogen found in soy products, is a highly specific inhibitor of protein tyrosine kinase (PTK) which blocks the mitogenic effect mediated by EGF on NIH-3T3 cells with IC50 of 12μM or by insulin with IC50 of 19 μM.

CAS No. 446-72-0

Selleck's Genistein (NPI 031L) has been cited by 26 publications

Purity & Quality Control

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EGFR Signaling Pathway Map

Biological Activity

Description

Targets

EGFR ^[1]

topo II ^[1]

In vitro

Genistein is an ATP competitive inhibitor. Genistein inhibits tyrosine phosphorylation in isolated enzyme and receptor preparations and in whole cells including platelets, lymphocytes and a variety of cultured cells. It also inhibits EGF-stimulated phosphorylation in cultured cells as well as inhibition of Topo II (topoisomerase II). Genistein inhibits EGF-stimulated tyrosine phosphorylation in cultured A431 epidermoid carcinoma cells. Inhibition is competitive with ATP and noncompetitive with substrate. ^[1] Genistein blocks the mitogenic effect mediated by EGF, insulin and thrombin on NIH-3T3 cells.^[2] Genistein also acts as an agonist at the GPR30 receptor and binds to PPARγ and estrogen receptors. Genistein also binds to PPARγ, acting as an agonist at this receptor with K_i of 5.7 μM. ^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

BxPC3	MlrHVJJwdGmoZYLheIlwdiCjc4PhfS=>			M1LRSGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQojQR\|Mh[2WubDDsbY5mKGK7IF3UWEBie3OjeTygTWM2OD1\|MDFOwG0>	MkGzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ5OEm3N\|coRjF4N{i5O\|M4RC:jPh?=
BT20	MWDQdo9tcW[ncnH0bY9vKGG\|c3H5			MWTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEKWMkCgZ4VtdCCuaX7lJIJ6KE2WVDDhd5NigSxiSVO1NF01PiEQvF2=	MoLPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ5OEm3N\|coRjF4N{i5O\|M4RC:jPh?=
ANN-1	M4LIfGN6fG:2b4jpZ{Bie3OjeR?=			NGnVdGlEgXSxdH;4bYMh\W[oZXP0JI9vKHZvYXLsJJRz[W6\|Zn;ycYVlKG23cnnu[UBCVk5vMTDj[YxteyxiSVO1NF05\|ryP	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85OjBzNkCzK\|45OjBzNkCzQE9iRg>?
breast carcinoma cells	MlnMR5l1d3SxeHnjJIF{e2G7			NHnPbGxEgXSxdH;4bYMh\W[oZXP0JI9vKE2FRj23JIh2dWGwIHLy[YF{fCClYYLjbY5wdWFiY3XscJMtKEmFNUC9NVUvOc7:TR?=	Mn3RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDJyMU[wN{c,QDJyMU[wN\|ww[T5?
3T3	MoDzR5l1d3SxeHnjJIF{e2G7			M4jse2N6fG:2b4jpZ{Bm\m[nY4Sgc44hO1R\|IHPlcIx{NCCLQ{WwQVI1\|ryP	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85OjBzNkCzK\|45OjBzNkCzQE9iRg>?
colon cells	MUXDfZRwfG:6aXOgZZN{[Xl?			Mn23R5l1d3SxeHnjJIVn\mWldDDvckBYcUS{IHj1cYFvKGOxbH;uJINmdGy\|LDDJR\|UxRTJ5LkhOwG0>	NFTVcJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96MkCxOlA{Lz56MkCxOlA{RC:jPh?=
Hepa1clc7	NXrjVlVXS3m2b4TvfIlkcXS7IHHzd4F6		M1njN\|czKGi{cx?=	MmnsR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgTIVx[TGlbHO3JINmdGy\|IHHmeIVzKDd{IHjyd{whUUN3ME2xNe69VQ>?	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB5NUe0Nkc,OTByN{W3OFI9N2F-
MCF7	NYLmd4tNTnWwY4Tpc44h[XO\|YYm=		M4PsblYhcHK\|	MoTWTY5pcWKrdHnvckBw\iCyaH;yZo9tKGW\|dHXyMYlv\HWlZXSgc5JvcXSqaX7lJIRm[2G{Yn;4fYxie2ViaX6gbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB4IHjyd{whUUN3ME2yOu69VQ>?	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB5NUe0Nkc,OTByN{W3OFI9N2F-
3T3/A31	NHXxWW5EgXSxdH;4bYNqfHliYYPzZZk>	MnXNNUB1dyBzMECgeYcwdUx?	MVu3NkBpenN?	NW\yV\|VRS3m2b4TvfIlkcXS7IHHnZYlve3RiQlHMRk9kKG2xdYPlJINtd26nZDCzWFMwSTNzIHPlcIx{KGG2IEGgeI8hOTByIIXnM41NKGGodHXyJFczKGi{czDifUBvcWe{b4PpckBie3OjeR?=	MmLhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByOU[4OlMoRjFyMEm2PFY{RC:jPh?=
SH-SY5Y	NYXMcm5XTnWwY4Tpc44h[XO\|YYm=			NH\OdHBC\2:waYP0JIFkfGm4aYT5JIlvKHS{YX7zZ5JqeHSrb37hcEBi[3SrdnH0bY9vKGG\|c3H5JIlvKFOKLWPZOXkhdmW3cn;icIF{fG:vYTDj[YxteyCneIDy[ZN{cW6pIFXzeJJw\2WwIILlZ4VxfG:{IHLleIEtKEWFNUC9NE4xODF5zszN	Mn7VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF7ME[yPFAoRjFzOUC2NlgxRC:jPh?=
HeLa	NFLnTIFHfW6ldHnvckBie3OjeR?=			MXLBZ5RqfmG2aX;uJI9nKGW\|dILv[4VvKHKnc4DvcpNmKGWuZX3lcpQhcW5iSHXMZUBk\WyuczDzeIFjdHlidILhcpNn\WO2ZXSge4l1cCCqdX3hckBGe3S{b3flckBz\WOncITvdkBj\XSjLjygSWM2OD1yLkCwOFHPxE1?	NHjNU4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUmwOlI5OCd-MUG5NFYzQDB:L3G+
HeLa	NX3hOoJSTnWwY4Tpc44h[XO\|YYm=			NIrlenpC[3SrdnH0bY9vKG:oIHXzeJJw\2WwIILld5BwdnOnIHXs[Y1mdnRiaX6gTIVN[SClZXzsd{B{fGGkbImgeJJidnOoZXP0[YQhf2m2aDDoeY1idiCHc4Tyc4dmdiC{ZXPldJRweiCjbIDoZU4tKEWFNUC9NE4xPDkQvF2=	Ml7zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF7ME[yPFAoRjFzOUC2NlgxRC:jPh?=
Ishikawa	MoruSZN1em:pZX7pZ{Bie3OjeR?=		NXzHZXF4PCCmYYnz	MmDsSZN1em:pZX7pZ{Bi[3Srdnn0fUBqdiCqdX3hckBKe2ira3H3ZUBk\WyuczDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZiYXzrZYxqdmVicHjvd5Bp[XSjc3WgZYN1cX[rdImgZYZ1\XJiNDDkZZl{KGK7IIDhdoEudmm2cn;wbIVvd2xicnXs[YF{\SCjc4PhfUwhUUN3ME2wMlUy\|ryP	NI\zO289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkWwNlMxPyd-MUK1NFI{ODd:L3G+
Hepa-1c1c7	NVPwUoU5TnWwY4Tpc44h[XO\|YYm=			MlTOTY5lfWO2aX;uJI9nKE6DRGDIPpF2cW6xbnWgdoVlfWO2YYPlJIlvKG2xdYPlJGhmeGFvMXOxZ\|ch[2WubIOgZZN{\XO\|ZXSgZZMh\HK3ZzDs[ZZmdCC{ZYH1bZJm\CC2bzDkc5VjdGViZX76fY1mKGGldHn2bZR6KGK7IF3UWEBie3OjeTygR2Q:OjJwOd88US=>	MkSwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR3MUC1PVAoRjF2NUGwOVkxRC:jPh?=
Hepa-1c1c7	M2nOcWZ2dmO2aX;uJIF{e2G7			Mk\0TY5pcWKrdHnvckBw\iCvb4Xz[UBJ\XCjLUHjNYM4KGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NF01PS5{zszN	NUHkS4xTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS1NVA2QTBpPkG0OVExPTlyPD;hQi=>
T47D	NEfxVIhGe3S{b3flcolkKGG\|c3H5			M3;BXWV{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hXDR5RDDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegd5RqdXWuYYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:wIHXxeYl3[WynboSgeI8hOTBicF2g[ZN1emGmaX;sJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gT1yLkCwNe69VQ>?	M{DOZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{i3OFM3Lz5zNUe4O\|Q{PjxxYU6=
MCF7	M2j3XWV{fHKxZ3XubYMh[XO\|YYm=			M{TTemV{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegd5RqdXWuYYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:wIHXxeYl3[WynboSgeI8hOTBicF2g[ZN1emGmaX;sJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gT1yLkCwN:69VQ>?	M4\pPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{i3OFM3Lz5zNUe4O\|Q{PjxxYU6=
T47D	NXjKZoNiTXO2cn;n[Y5q[yCjc4PhfS=>			NEXafIFGe3S{b3flcolkKGGldHn2bZR6KGmwIHj1cYFvKFR2N1SgZ4VtdHNiYYPz[ZN{\WRiYYOg[JJ2\yCuZY\lcEBk[XW\|aX7nJJN1cW23bHH0bY9vKG:oIHPlcIwheHKxbHnm[ZJifGmxbjDldZVqfmGuZX70JJRwKDFyMDDwUUBme3S{YXTpc4wh[nliYXzhcYFzKGKudXWgZZN{[XluIFHjeIl3cXS7PUCuNFA6\|ryP	NWX5RYVpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3PFc1OzZpPkG1O\|g4PDN4PD;hQi=>
MCF7	NX6ySZR[TXO2cn;n[Y5q[yCjc4PhfS=>			MXfFd5Rzd2enbnnjJIFkfGm4aYT5JIlvKGi3bXHuJG1ETjdiY3XscJMh[XO\|ZYPz[YQh[XNiZIL1[{Bt\X[nbDDjZZV{cW6pIIP0bY12dGG2aX;uJI9nKGOnbHygdJJwdGmoZYLheIlwdiCncYXpeoFt\W62IITvJFExOCCyTTDld5Rz[WSrb3ygZpkh[WyjbXHyJIJtfWViYYPzZZktKEGldHn2bZR6RTBwMEGz{txO	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd6N{SzOkc,OTV5OEe0N\|Y9N2F-
293T	M4jCeWZ2dmO2aX;uJIF{e2G7			MorWSIl{eGyjY3Xt[Y51KG:oIGuzTH0yP2KndHGt[ZN1emGmaX;sJIZzd21icnXjc41jcW6jboSgbJVu[W5iRWLi[ZRiKGW6cILld5Nm\CCrbjCyPVNVKGOnbHzzMEBKSzVyPUCuNFAyO87:TR?=	M1nRcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4MkG5OFY{Lz5zNkKxPVQ3OzxxYU6=
293T	Mn7aSpVv[3Srb36gZZN{[Xl?			M{XtSmRqe3CuYXPlcYVvfCCxZjDbN2heOTekZYThMYV{fHKjZHnvcEBnem:vIILlZ49u[mmwYX70JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJFI6O1RiY3XscJMtKEmFNUC9NE4xOjl2zszN	MnvuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ{MUm0OlMoRjF4MkG5OFY{RC:jPh?=
Vero	NY\OU3NYS3m2b4TvfIlkcXS7IHHzd4F6			NFmwRVdEgXSxdH;4bYNqfHliYXfhbY5{fCCjZoLpZ4FvKGe{ZXXuJI1wdmuneTDW[ZJwKGOnbHzzMEBKSzVyPUOyMlnPxE1?	NEXteoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkS0NVA3Pid-MU[0OFExPjZ:L3G+
U937	MVHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MUS3NkBpenN?	MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV7M{egZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KFeVVD24JIF{e2G7LDDJR\|UxRTR6zszN	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF3OEC1OEc,OTdzNUiwOVQ9N2F-
RAW 264	NX65ZnVNTnWwY4Tpc44h[XO\|YYm=		MVKyOEBpenN?	M3;MUGlvcGmkaYTpc44hd2ZiMTD1[{9udCCOUGOtd5RqdXWuYYTl[EBVVk[jbIDoZUBi[2O3bYXsZZRqd25iaX6gVmFYKDJ4NDDj[YxteyCjZoTldkAzPCCqcoOsJGlEPTB;MUiuNe69VQ>?	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN{MEK0Okc,OTd\|MkCyOFY9N2F-
K562	NF2wZVVIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		Ml;IOUBl[Xm\|	NUPvVXlKT3Kxd4ToJIlvcGmkaYTpc44hd2ZiS{W2NkBk\WyuczDifUBZXFRiYYPzZZkh[W[2ZYKgOUBl[Xm\|LDDJR\|UxRTF5LkW2{txO	NVvqZVV2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVExQTJpPkG3OFEyODl{PD;hQi=>
HUVE12	MonlSpVv[3Srb36gZZN{[Xl?			MlvPVoV3\XK\|YXygc4YhcHmmcn;n[Y4heGW{b4jp[IUucW6mdXPl[EBNTEhiYXP0bZZqfHliaX6gbJVu[W5iSGXWSVEzKGOnbHzz	NVPJTIxHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiwOlg6QDBpPkG4NFY5QThyPD;hQi=>
HL60	NX;FXHVESW62aXzleYtmdWmlIHHzd4F6		NWPqUWpLOjRiaILz	NFnrdo1CdnSrbHX1b4VucWNiYXP0bZZqfHliYXfhbY5{fCCqdX3hckBJVDZyIHPlcIx{KGGodHXyJFI1KGi{czDifUBkdG:wb3flcolkKGG\|c3H5MEBNSzVyPU[uN:69VQ>?	M1ewOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MU[zOVg6Lz5zOEG2N\|U5QTxxYU6=
L1210	MXzBcpRqdGW3a3XtbYMh[XO\|YYm=		MmrXNlQhcHK\|	NYCxN4xiSW62aXzleYtmdWmlIHHjeIl3cXS7IHHnZYlve3RibX;1d4UhVDF{MUCgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KGOub37v[4VvcWNiYYPzZZktKEyFNUC9Ok42\|ryP	MoPBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThzNkO1PFkoRjF6MU[zOVg6RC:jPh?=
MOLT3	MlTHRY51cWyndXvlcYlkKGG\|c3H5		MmC1NlQhcHK\|	NH\FTYNCdnSrbHX1b4VucWNiYXP0bZZqfHliYXfhbY5{fCCqdX3hckBOV0yWMzDj[YxteyCjZoTldkAzPCCqcoOgZpkh[2yxbn;n[Y5q[yCjc4PhfUwhVEN3ME23MlXPxE1?	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDF4M{W4PUc,OThzNkO1PFk9N2F-
MOLT3	MoO3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		M4m1eFQ5KGi{cx?=	MYjHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNU2xVOyClZXzsd{Bi\nSncjC0PEBpenNuIFnHOVA:OTJwN988US=>	MnvIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThzNkO1PFkoRjF6MU[zOVg6RC:jPh?=
Raji	MXHBcpRqdGW3a3XtbYMh[XO\|YYm=		NVrDcotPOjRiaILz	MnjqRY51cWyndXvlcYlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gVoFrcSClZXzsd{Bi\nSncjCyOEBpenNiYomgZ4xwdm:pZX7pZ{Bie3OjeTygUGM2OD1zMz63{txO	Mn3rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThzNkO1PFkoRjF6MU[zOVg6RC:jPh?=
HL60	MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		MY[0PEBpenN?	MnfKS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTGw3OCClZXzsd{Bi\nSncjC0PEBpenNuIFnHOVA:OThwMt88US=>	NGniR4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEG2N\|U5QSd-MUixOlM2QDl:L3G+
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MDCK	MnHlSpVv[3Srb36gZZN{[Xl?			NVLoUXF{UW6qaXLpeIlwdiCxZjDCR3JRKGW6cILld5Nm\CCrbjDNSGNMKGOnbHzzJJV{cW6pIFjv[YNpe3RiM{OzOFIhe3SjaX7pcoctKEmFNUC9Ok46\|ryP	NIPIXmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO1OFgxOCd-MkGzOVQ5ODB:L3G+
MCF-7 MX	NYfGTHF5TnWwY4Tpc44h[XO\|YYm=			NXL3bYpRUW6qaXLpeIlwdiCxZjDCR3JRKGW6cILld5Nm\CCrbjDNR2YuPyCPWDDj[YxteyC3c3nu[{BJd2WlaIP0JFM{OzR{IIP0ZYlvcW6pLDDJR\|UxRThwON88US=>	Mo\qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF\|NUS4NFAoRjJzM{W0PFAxRC:jPh?=
NCI-ADR-RES	M{HW[mFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NF7NU4U1QCCqcoO=	M4HJXWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[ZN1em:pZX6gdoVk\XC2b4Kt[IVncWOrZX70JIh2dWGwIF7DTU1CTFJvUlXTJINmdGy\|IHnuZ5Vj[XSnZDD3bZRpKDBwME[gcm0hd2ZiZYP0doFlcW:uIHHu[EAxNjB5IH7NJI9nKHSnc4Tvd5Rmem:wZTDh[pRmeiB2ODDodpMh[nlic4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUBqdiCycnXz[Y5k\SCxZjDm[ZRidCCkb4\pcoUhe2W{dX2gZY5lKE63U3XyeY0tKEeLNUC9NVYvQTh{NN88US=>	NGLzemg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxN\|I4PSd-MkG1NVMzPzV:L3G+
MCF12A	Mmf3RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NFjxWZY1QCCqcoO=	MVrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCPQ1[xNmEh[2WubIOgbY5kfWKjdHXkJJdqfGhiMD6wOkBvVSCxZjDld5Rz[WSrb3ygZY5lKDBwMEegcm0hd2ZidHXzeI9{fGW{b37lJIFnfGW{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJIZmfGGuIHLveolv\SC\|ZYL1cUBidmRiToXT[ZJ2dSxiR1m1NF0zOS56N{e2{txO	M3rYPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlc2Lz5{MUWxN\|I4PTxxYU6=
NCI-ADR-RES	NEPlXW9CdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NUXRWJhlPDhiaILz	MXnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCQQ1mtRWRTNVKHUzDj[YxteyCrbnP1ZoF1\WRid3n0bEAyODBibl2gc4Yh\XO2cnHkbY9tKGGwZDCwMlA4KG6PIH;mJJRme3Sxc4Tldo9v\SCjZoTldkA1QCCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTDpckBxemW\|ZX7j[UBw\iCoZYThcEBjd3[rbnWgd4VzfW1iYX7kJG52W2W{dX2sJGdKPTB;Mk[uPVE2PM7:TR?=	M4PIeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlc2Lz5{MUWxN\|I4PTxxYU6=
MCF12A	NYrsNFJ2SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NUGwOIVoPDhiaILz	MVXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCPQ1[xNmEh[2WubIOgbY5kfWKjdHXkJJdqfGhiMUCwJI5OKG:oIHXzeJJi\GmxbDDhcoQhOC5yNzDuUUBw\iC2ZYP0c5N1\XKxbnWgZYZ1\XJiNEigbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJiYYPzZZkhcW5icILld4Vv[2Vib3[g[oV1[WxiYn;2bY5mKHOncoXtJIFv\CCQdWPldpVuNCCJSUWwQVMxNjF7OUZOwG0>	NHz2OIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxN\|I4PSd-MkG1NVMzPzV:L3G+
NCI-ADR-RES	MUXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		M2TwdFQ5KGi{cx?=	MlrwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDld5Rzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iTlPJMWFFWi2URWOgZ4VtdHNiaX7jeYJifGWmIIfpeIghOC5yMEOgcm0hd2ZiZYP0doFlcW:uIHHu[EAxNjBzIH7NJI9nKHSnc4Tvd5Rmem:wZTDh[pRmeiB2ODDodpMh[nlic4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUBqdiCycnXz[Y5k\SCxZjC1KUBn\XSjbDDic5ZqdmVic3XyeY0tKEeLNUC9N\|IvOzV7NN88US=>	NVP0TIZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzPzVpPkKxOVE{Ojd3PD;hQi=>
MCF12A	NYDsc4RZSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MUi0PEBpenN?	MmCxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDld5Rzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iTVPGNVJCKGOnbHzzJIlv[3WkYYTl[EB4cXSqIECuNFA{KG6PIH;mJIV{fHKjZHnvcEBidmRiMD6wNUBvVSCxZjD0[ZN1d3O2ZYLvcoUh[W[2ZYKgOFghcHK\|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XliaX6gdJJme2WwY3Wgc4YhPSViZnX0ZYwh[m:4aX7lJJNmenWvLDDHTVUxRTN{LkO1PVTPxE1?	NYX0Z\|BtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzPzVpPkKxOVE{Ojd3PD;hQi=>
LNCAP	NVLSNGV2SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MWC0PEBpenN?	NHXXNYFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzOR2FRKGOnbHzzJIV5eHKnc4Ppcoch[W6mcn;n[Y4hemWlZYD0c5IhcW6ldXLheIVlKHerdHigNE43KG6PIH;mJIV{fHKjZHnvcEBidmRiMD63JI5OKG:oIITld5Rwe3Sncn;u[UBi\nSncjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiR1m1NF0{QC57MES1{txO	NGHJdmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxN\|I4PSd-MkG1NVMzPzV:L3G+
PC3	M17yTWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MoHwOFghcHK\|	MmXjRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDhcoRzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iUFOzJINmdGy\|IHnuZ5Vj[XSnZDD3bZRpKDBwNjDuUUBw\iCnc4TyZYRqd2xiYX7kJFAvPyCwTTDv[kB1\XO2b4P0[ZJwdmViYX\0[ZIhPDhiaILzJIJ6KHO3bH\vdohw\GGvaX7lJGIh[XO\|YYmsJGdKPTB;NEKuOlU4Qc7:TR?=	MnzQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUOyO\|UoRjJzNUGzNlc2RC:jPh?=
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FRT	MkH5SpVv[3Srb36gZZN{[Xl?		MYCyOEBpenN?	NFP1VG9DcW6maX7nJIFn\mmwaYT5JJRwKEOIVGKgSlUxQCCmZXzleIlwdiCvdYThcpQh\XiycnXzd4VlKGmwIH\vdpNsd2yrbj3zeIlufWyjdHXkJGZTXCClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDpc4RqdmViaX7mcJV5KG2nYYP1doVlKGG\|IGnGVEByfWWwY3jpcochemG2ZTDh[pRmeiB{NDDodpMh[nliZnz1c5Jme2OnbnPlJIF{e2G7LDDL[F06NjUQvF2=	NFXqXlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUW2PFMzOyd-MkG1Olg{OjN:L3G+
MCF7	MofvRY51cWW\|dILv[4VvcWNiYYPzZZk>	MXqxJJRwKDJyIIXN	MkLBOFghcHK\|	MXLBcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCHUj3wc5NqfGm4ZTDNR2Y4KGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gNVdj\XSjIHXzeJJi\GmxbD3pcoR2[2WmIIPlZ5JmfGWmIHHsb4FtcW6nIIDoc5NxcGG2YYPlJIFkfGm4aYT5JIF1KDFidH:gNlAhfU1iYX\0[ZIhPDhiaILzJIJ6KHCqb4PwbIEudGmpaISgdoVxd3K2ZYKgZ4hmdWmudX3pcoV{[2WwY3WgZZN{[Xl?	Ml3nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6MEC4OVkoRjJzOECwPFU6RC:jPh?=
Jurkat T	MmPVRZBweHSxc3nzJIF{e2G7		NG\xN2Y{PiCqcoO=	NULuS40xUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDKeZJs[XRiVDDj[YxteyCxdnXy[ZhxemW\|c3nu[{BP\W9iYX\0[ZIhOzZiaILzJIJ6KG[ub4egZ5l1d22ndIL5	NWHacG5oRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxPVc{QTNpPkKyNVk4Ozl\|PD;hQi=>
FRT	NGDpN49HfW6ldHnvckBie3OjeR?=			MVjQc5RmdnSrYYTpc44h[WO2aY\peJkh[XRiaIXtZY4hS0[WUjDk[Yx1[SCINUC4JI12fGGwdD3t[YRq[XSnZDDpc4RqdmViZnz1fEBmgHC{ZYPz[YQhcW5icnH0JGZTXCClZXzsd{Bkd2W6cILld5NqdmdiZnz1c5Jme2OnboSgdJJwfGWrbjDZSnAuUDF2OGGvNVE2OkxiYomg[ox2d3Knc3PlcoNmKGG\|c3H5MEBGSzVyPUhOwG0>	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ6MUG4OUc,OjJ{OEGxPFU9N2F-
Human Huh7.5.1	MYPBcpRqfmm{YXygZZN{[Xl?		NGTNWGc4OiCqcoO=	NYLPc25ISW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEOYIFrGTE0yKEp\|OUnFUUBqdm[nY4Tl[EBqdiCKdX3hckBJfWh5LkWuNUBk\WyuczDhd5Nme3OnZDDhd{B{fXCycnXzd4lwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDh[pRmeiB5MjDodpMh[nliRVfGVEBie3OjeTygSWM2OD1zND60{txO	M1\iZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NES1N\|I5Lz5{MkS0OVMzQDxxYU6=
CHO	MWjGeY5kfGmxbjDhd5NigQ>?			Mle2T4khfmGudXXzJIZweiC\|b3TpeY0h\my3b4Lld4NmcW5iKEGwJJVOMSC3cIThb4UhcW5iT1HUVFFDOy22cnHud4Zm[3SnZDDDTG8h[2WubIOsJGtqRTJwOUdOwG0>	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV5MUSxOUc,OjN3N{G0NVU9N2F-
CHO	NX;qSnU6TnWwY4Tpc44h[XO\|YYm=			NVj5fFBVeEmFNUCgeoFtfWW\|IH\vdkB{d2SrdX2g[ox2d3Knc3PlbY4hMDFyIIXNLUB2eHSja3WgbY4hV0GWUEHCN{11emGwc3\lZ5Rm\CCFSF:gZ4VtdHNuIFnDOVA:Oy56OUC0Oe69VQ>?	MkLXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3N{G0NVUoRjJ\|NUexOFE2RC:jPh?=
CHO	NWD4SJBqTnWwY4Tpc44h[XO\|YYm=			NUXYeWREU2lidnHseYV{KG[xcjDzc4RqfW1iZnz1c5Jme2OnaX6gLFExKHWPKTD1dJRic2ViaX6gU2FVWDGEMT30doFve2[nY4Tl[EBEUE9iY3XscJMtKEurPUZOwG0>	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV5MUSxOUc,OjN3N{G0NVU9N2F-
CHO	NWTqNItzTnWwY4Tpc44h[XO\|YYm=			M4L5[5BKSzVyII\hcJVmeyCob4Kgd49lcXWvIH\seY9z\XOlZXnuJEgyOCC3TTmgeZB1[WunIHnuJG9CXFBzQkGteJJidnOoZXP0[YQhS0iRIHPlcIx{NCCLQ{WwQVkvOTJyMUJOwG0>	M1zHSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NUexOFE2Lz5{M{W3NVQyPTxxYU6=
HuH7	MYjBcpRqeGyjc33v[IlidCCjc4PhfS=>		NXv6bm5tOjRiaILz	M1PZd2FvfGmybHHzcY9lcWGuIHHjeIl3cXS7IHHnZYlve3RibHn2[ZIhe3Cxcn;6c4l1\SC\|dHHn[UBw\iCSbHHzcY9lcXWvIHLldodp\WliQV7LRUBmgHC{ZYPzbY5oKEeIUDDpcoZm[3SnZDDpckBpfW2jbjDIeWg4KGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gdIFz[XOrdHWg[IV3\WyxcH3lcpQh[W[2ZYKgNlQhcHK\|IHL5JJFTXC2SQ2KgZY5idHm\|aYOsJGlEPTB;MkFOwG0>	NGLUbYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGyOVg1QSd-MkSxNlU5PDl:L3G+
SKBR3	MYXD[YxtKGO7Y3zlJIF{e2G7	NYP0XoczPTBidH:gO\|UhfU1?	MnrtN\|YhcHK\|	MkHQR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gV2tDWjNiY3XscJMh[XO\|ZYPz[YQh[XNiZHXjdoVie2ViaX6gZYNkfW23bHH0bY9vKGG2IFewM2cyKHCqYYPlJIF1KDVyIITvJFc2KHWPIHHmeIVzKDN4IHjyd{BjgSCycn;wbYRqfW1iaX;kbYRmKHO2YXnubY5oNWKjc3XkJIZtd3diY4n0c41mfHKrYzDhcoFtgXOrcx?=	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR3NkCwOEc,OjR2NU[wNFQ9N2F-
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J774A1	M3G5bWZ2dmO2aX;uJIF{e2G7	MWexN{B2VQ>?		M{TMdWlvcGmkaYTpc44hd2Zibnn0dolkKG:6aXTlJJBzd2S3Y4Tpc44hcW5iTGDTM2lHVmejbX3hMZN1cW23bHH0[YQhdW:3c3WgTlc4PEFzIHPlcIx{KGG\|c3Xzd4VlKGG\|IH7peJJqfGW\|IHzleoVtKGG2IEGzJJVOKGK7IFfybYV{eyCvZYToc4QhMFK4YjC9JFE3NjViKz:tJFEvPCC3TTmsJGFkfGm4aYT5QVE{NjkQvF2=	NUHoUVVVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlcyPTNpPkK1NVI4OTV\|PD;hQi=>
J774A1	M2DMSmZ2dmO2aX;uJIF{e2G7	NVz2PXJnOjZidV2=		NXPZVIoyUW6qaXLpeIlwdiCxZjDubZRzcWNib4jp[IUheHKxZIXjeIlwdiCrbjDMVHMwUU[QZ3HtcYEue3SrbYXsZZRm\CCvb4Xz[UBLPzd2QUGgZ4VtdHNiYYPz[ZN{\WRiYYOgcol1emm2ZYOgcIV3\WxiYYSgNlYhfU1iYomgS5Jq\XO\|IH3leIhw\CBqUo\iJF0hOTZwNTCrM{0hOS52IIXNLUwhSWO2aY\peJk:OTRwNd88US=>	NIOzdVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyO\|E2Oyd-MkWxNlcyPTN:L3G+
HEK293	NF;6PGtHfW6ldHnvckBie3OjeR?=			M1PBVmlvcGmkaYTpc44hd2ZiRVfGVkApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZZN{\XO\|ZXSgZZMh\GWlcnXhd4UhcW5icHjvd5Bpd3K7bHH0bY9vKGK7IHPo[Y1qdHWvaX7ld4NmdmOnIHHzd4F6NCCLQ{WwQVI2NjF{zszN	NELX[\|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUKwOVE6OCd-MkWyNFUyQTB:L3G+
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NCI-H460	M3P5XGN6fG:2b4jpZ4l1gSCjc4PhfS=>		MVS0PEBpenN?	NVH6XIFRS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVkOLLVi0OlAh[2WubIOgZZN{\XO\|ZXSgZZMh\GWlcnXhd4UhcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK\|IHL5JG1VXCCjc4PhfUwhUUN3ME20O:69VQ>?	MmO5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4NUSyOlUoRjJ6NkW0NlY2RC:jPh?=
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LNCAP	NWTKRXByTnWwY4Tpc44h[XO\|YYm=	MWSyMlUhfU1?	Ml;tNlQhcHK\|	MmfPTY5pcWKrdHnvckBw\iCKRFHDOk9JW1B7MDDpckBpfW2jbjDMUmNCWCClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCDUjDwdo91\WmwIHzleoVtKGG2IEKuOUB2VSCjZoTldkAzPCCqcoOgZpkhX2W\|dHXyckBjdG:2IH3leIhw\A>?	MormQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2NU[xNVMoRjJ7NEW2NVE{RC:jPh?=
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MDA-MB-231/beta41	MXvFd5Rzd2enbnnjJIF{e2G7		NHrFfIUyQCCqcoO=	MVzFd5Rzd2enbnnjJIFkfGm4aYT5JIF1KEWUYnX0ZUApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCqdX3hckBOTEFvTVKtNlMyN2KndHG0NUBk\WyuczDh[pRmeiBzODDodpMh[nlicnXubYxt[SCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTB;MD6wNFI1\|ryP	NFPDPWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[0NVIxPid-Mkm2OFEzODZ:L3G+
Ishikawa	NYXyT4lvTXO2cn;n[Y5q[yCjc4PhfS=>		M2DYZ\|k3KGi{cx?=	MWPFd5Rzd2enbnnjJIFkfGm4aYT5JIF1KEWUYXzwbIEhcW5iaIXtZY4hUXOqaXvhe4Eh[2WubIOgZZN{\XO\|ZXSgZZMhcW6mdXP0bY9vKG:oIHHsb4FtcW6nIIDoc5NxcGG2YYPlJIFkfGm4aYT5JJV{cW6pIICtcol1em:yaHXuc4wh[XNic4Xid5Rz[XSnIITy[YF1\WRiZn;yJFk3KGi{czDmc4xtd3enZDDifUB{fWK\|dILheIUh[WSmaYTpc44h[nlic4DlZ5Rzd3Cqb4TvcYV1emmlIH3leIhw\CxiRVO1NF0xNjJ2zszN	M37QT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkSxNlA3Lz5{OU[0NVIxPjxxYU6=
HEK293	NFTrXohHfW6ldHnvckBie3OjeR?=		M3vae\|Q5KGi{cx?=	NVLue4JGSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWi2kZYThJJRz[W6\|ZnXjeIVlKGmwIFjFT\|I6OyClZXzsd{Bi\nSncjC0PEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODF7OUZOwG0>	Ml\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQwLz6FaFXNRmw9N2F-
HEK293	Ml\1SpVv[3Srb36gZZN{[Xl?		MX:0PEBpenN?	MWrB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSMYFteGijIITyZY5{\mWldHXkJIlvKEiHS{K5N{Bk\WyuczDh[pRmeiB2ODDodpMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyPUCuOVAyOToQvF2=	M4jiT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1Nyd-Q3jFUWJNRC:jPh?=
HT-29	NFrzUGZIem:5dHigbY5pcWKrdHnvckBie3OjeR?=			MnPyS5Jwf3SqIHnubIljcXSrb36gc4YhUG:vbzDzZZBq\W6\|IDjoeY1idiliSGStNlkh[2WubIOgZpkhVVSWIHHzd4F6NCCJSU20MlEy\|ryP	Mki1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQwLz6FaFXNRmw9N2F-
HL60	M{XJWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7			NHjic45Iem:5dHigbY5pcWKrdHnvckBw\iCKb33vJJNieGmnboOgLIh2dWGwKTDIUFYxKGOnbHzzJIJ6KE2WVDDhd5NigSxiR1m9OE45Os7:TR?=	M2\I[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1Nyd-Q3jFUWJNRC:jPh?=
SGC7901	M2PETWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7			MoLtS5Jwf3SqIHnubIljcXSrb36gc4YhUG:vbzDzZZBq\W6\|IDjoeY1idiliU1fDO\|kxOSClZXzsd{BjgSCPVGSgZZN{[XluIFfJQVUvPzkQvF2=	MlG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQwLz6FaFXNRmw9N2F-
HL60	MWrGeY5kfGmxbjDhd5NigQ>?			Ml3YR49ueGW2aYTpeoUhcW6qaXLpeIlwdiCxZjDHUHVVOSCrbjDoeY1idiCKTE[wJINmdGy\|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiB{LWuxMFIuO0ifZHXvfJkuTC2pbIXjc5NmKHWydHHr[UBu\WG\|dYLl[EBnd3JiM{Cgd4VkeyCkeTDkc5VjdGVicnXjbZBzd2OjbDDwcI91KGGwYXz5d4l{NCCNaU23{txO	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OE8oRkOqRV3CUFww[T5?
CHO	Mn7HSpVv[3Srb36gZZN{[Xl?			MnnrR49ueGW2aYTpeoUhcW6qaXLpeIlwdiCxZjDHUHVVOSBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwIEKtX\|EtOi1\|SG3k[Y95gS2GLXfseYNwe2VidYD0ZYtmKG2nYYP1doVlKG[xcjCzNEB{\WO\|IHL5JIRwfWKuZTDy[YNqeHKxY3HsJJBtd3RiYX7hcJl{cXNuIFvpQVfPxE1?	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OE8oRkOqRV3CUFww[T5?
3T3L1	NHP3dIdHfW6ldHnvckBie3OjeR?=			NXzOfo14UW6qaXLpeIlwdiCxZjDHUHVVPCCrbjDtc5V{\SB\|VEPMNUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iaX7zeYxqdi2\|dHnteYxifGWmIEKt[IVwgHlvRD3bNVREZWeudXPvd4UhfXC2YXvlMEBKSzVyPUKw{txO	NFO0Xo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PC9pPlPoSW1DVDxxYU6=

Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	PPARγ / C/EBPα / αP2 ; p-ERK ; Foxo3 ; CIP2A / PARP / Cleaved PARP / Caspase-3 / Cleaved Caspase-3 ; p-AKT / AKT ; DNMT-1 / DNMT-3a / DNMT-3b	18384126 21639915 27574003 25931004
Immunofluorescence	ER-β / pS87 ER-β	25931004

In vivo

Genistein has chemopreventive effects on breast, prostate, and other endocrine-dependent tumors in adult animals. Genistein in the diet reduced the incidence of poorly differentiated prostatic adenocarcinomas in a dose-dependent manner and down-regulated androgen receptor, estrogen receptor-alpha, progesterone receptor, epidermal growth factor receptor, insulin-like growth factor-I, and extracellular signal-regulated kinase-1 but not estrogen receptor-beta and transforming growth factor-alpha mRNA expressions. Dietary genistein protects against mammary and prostate cancers by regulating specific sex steroid receptors and growth factor signaling pathways.^[4] Genistein combined with prostate tumor irradiation causes greater inhibition of primary tumor growth and increases control of spontaneous metastasis to para-aortic lymph nodes, increasing mouse survival. Paradoxically, treatment with genistein alone increases metastasis to lymph nodes. ^[5]

Protocol (from reference)

References

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Solubility (25°C)

In vitro

Chemical Information

Molecular Weight	270.24
Formula	C₁₅H₁₀O₅
CAS No.	446-72-0
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	C1=CC(=CC=C1C2=COC3=CC(=CC(=C3C2=O)O)O)O

Download Genistein (NPI 031L) SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

Clinical Trial Information

NCT Number	Recruitment	Interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05073523	Recruiting	Other: Diet	Healthy	Chalmers University of Technology	September 27 2021	Not Applicable
NCT01982578	Completed	Dietary Supplement: Genistein\|Other: Placebo	Alzheimer''s Disease	Fundación para la Investigación del Hospital Clínico de Valencia\|University of Valencia	September 1 2017	Not Applicable
NCT02499861	Completed	Drug: Decitabine and Genistein	Cancer	St. Justine''s Hospital	July 2015	Phase 1\|Phase 2
NCT01628471	Completed	Drug: decitabine in combination with genistein	Non Small Cell Lung Cancer	Uman Pharma\|DSM Nutritional Products Inc.\|MDEIE Ministry Québec Government\|INRS-Institut Armand Frappier Université du Québec	November 2012	Phase 1\|Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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