Genistein (NPI 031L)

For research use only.

Catalog No.S1342

20 publications

Genistein (NPI 031L) Chemical Structure

CAS No. 446-72-0

Genistein (NPI 031L), a phytoestrogen found in soy products, is a highly specific inhibitor of protein tyrosine kinase (PTK) which blocks the mitogenic effect mediated by EGF on NIH-3T3 cells with IC50 of 12μM or by insulin with IC50 of 19 μM.

Selleck's Genistein (NPI 031L) has been cited by 20 publications

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Biological Activity

Description Genistein (NPI 031L), a phytoestrogen found in soy products, is a highly specific inhibitor of protein tyrosine kinase (PTK) which blocks the mitogenic effect mediated by EGF on NIH-3T3 cells with IC50 of 12μM or by insulin with IC50 of 19 μM.
Targets
EGFR [1] topo II [1]
In vitro

Genistein is an ATP competitive inhibitor. Genistein inhibits tyrosine phosphorylation in isolated enzyme and receptor preparations and in whole cells including platelets, lymphocytes and a variety of cultured cells. It also inhibits EGF-stimulated phosphorylation in cultured cells as well as inhibition of Topo II (topoisomerase II). Genistein inhibits EGF-stimulated tyrosine phosphorylation in cultured A431 epidermoid carcinoma cells. Inhibition is competitive with ATP and noncompetitive with substrate. [1] Genistein blocks the mitogenic effect mediated by EGF, insulin and thrombin on NIH-3T3 cells.[2] Genistein also acts as an agonist at the GPR30 receptor and binds to PPARγ and estrogen receptors. Genistein also binds to PPARγ, acting as an agonist at this receptor with Ki of 5.7 μM. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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ANN-1 M{S0O2N6fG:2b4jpZ{Bie3OjeR?= NEXHS4ZEgXSxdH;4bYMh\W[oZXP0JI9vKHZvYXLsJJRz[W6|Zn;ycYVlKG23cnnu[UBCVk5vMTDj[YxteyxiSVO1NF05|ryP Ml;3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDJyMU[wN{c,QDJyMU[wN|ww[T5?
breast carcinoma cells NWjzVZpnS3m2b4TvfIlkKGG|c3H5 MUfDfZRwfG:6aXOg[YZn\WO2IH;uJG1ETi15IHj1cYFvKGK{ZXHzeEBk[XKlaX7vcYEh[2WubIOsJGlEPTB;MUWuNe69VQ>? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek85OjBzNkCzK|45OjBzNkCzQE9iRg>?
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Ishikawa M3qzeGV{fHKxZ3XubYMh[XO|YYm= Mlr5OEBl[Xm| NELLeWtGe3S{b3flcolkKGGldHn2bZR6KGmwIHj1cYFvKEm|aHnrZZdiKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDhcItidGmwZTDwbI9{eGijdHHz[UBi[3Srdnn0fUBi\nSncjC0JIRigXNiYomgdIFz[S2waYTyc5Bp\W6xbDDy[Yxm[XOnIHHzd4F6NCCLQ{WwQVAvPTIQvF2= M2PkflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{NUCyN|A4Lz5zMkWwNlMxPzxxYU6=
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MCF-7 MX MUnGeY5kfGmxbjDhd5NigQ>? MVTJcohq[mm2aX;uJI9nKEKFUmCg[ZhxemW|c3XkJIlvKE2FRj23JG1ZKGOnbHzzJJV{cW6pIFjv[YNpe3RiM{OzOFIhe3SjaX7pcoctKEmFNUC9PE45|ryP NV;RcVZjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzOVQ5ODBpPkKxN|U1QDByPD;hQi=>
NCI-ADR-RES M{TUSWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NUXETYxyPDhiaILz MYXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCQQ1mtRWRTNVKHUzDj[YxteyCrbnP1ZoF1\WRid3n0bEAxNjB4IH7NJI9nKGW|dILh[IlwdCCjbnSgNE4xPyCwTTDv[kB1\XO2b4P0[ZJwdmViYX\0[ZIhPDhiaILzJIJ6KHO3bH\vdohw\GGvaX7lJGIh[XO|YYmgbY4heHKnc3XuZ4Uhd2ZiZnX0ZYwh[m:4aX7lJJNmenWvIHHu[EBPfVOncoXtMEBIUTVyPUG2Mlk5OjUQvF2= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
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NCI-ADR-RES NF;adXdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M3PxV|Q5KGi{cx?= NUKx[HViSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBme3S{b3flckBz\WOncITvdk1l\W[rY3nlcpQhcHWvYX6gUmNKNUGGUj3SSXMh[2WubIOgbY5kfWKjdHXkJJdqfGhiMUCwJI5OKG:oIHXzeJJi\GmxbDDhcoQhOC5yNzDuUUBw\iC2ZYP0c5N1\XKxbnWgZYZ1\XJiNEigbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJiYYPzZZkhcW5icILld4Vv[2Vib3[g[oV1[WxiYn;2bY5mKHOncoXtJIFv\CCQdWPldpVuNCCJSUWwQVI3NjlzNUVOwG0> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
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MCF7 M2HReGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MUK0PEBpenN? M1fFWWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDlfJBz\XO|aX7nJIV{fHKxZ3XuJJJm[2WydH;yJIlv[3WkYYTl[EB4cXSqIECuNFYhdk1ib3[g[ZN1emGmaX;sJIFv\CByLkC3JI5OKG:oIITld5Rwe3Sncn;u[UBi\nSncjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSCrbjDwdoV{\W6lZTDv[kBn\XSjbDDic5ZqdmVic3XyeY0h[W6mIF71V4VzfW1uIFfJOVA:PDVwN{C4PO69VQ>? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
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MCF7 M1fXO2FvfGmnc4Tyc4dmdmmlIHHzd4F6 M1L6bVEhfG9iMkCgeW0> NFr2Z201QCCqcoO= MX7BcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCHUj3wc5NqfGm4ZTDNR2Y4KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gNVdj\XSjIHXzeJJi\GmxbD3pcoR2[2WmIIPlZ5JmfGWmIHHsb4FtcW6nIIDoc5NxcGG2YYPlJIFkfGm4aYT5JIF1KDFidH:gNlAhfU1iYX\0[ZIhPDhiaILzJIJ6KHCqb4PwbIEudGmpaISgdoVxd3K2ZYKgZ4hmdWmudX3pcoV{[2WwY3WgZZN{[Xl? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOThyMEi1PUc,OjF6MEC4OVk9N2F-
Jurkat T MorERZBweHSxc3nzJIF{e2G7 MnrBN|YhcHK| MmP2TY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCMdYLrZZQhXCClZXzsd{BwfmW{ZYjwdoV{e2mwZzDO[Y8h[W[2ZYKgN|YhcHK|IHL5JIZtd3diY4n0c41mfHK7 NYLuUIlyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxPVc{QTNpPkKyNVk4Ozl|PD;hQi=>
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Human Huh7.5.1 NV;lTVRqSW62aY\pdoFtKGG|c3H5 NHi4PJc4OiCqcoO= NHLHZWdCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJS1ZiSl\IMVEhUjN7OVXNJIlv\mWldHXkJIlvKEi3bXHuJGh2cDdwNT6xJINmdGy|IHHzd4V{e2WmIHHzJJN2eHC{ZYPzbY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wIHHmeIVzKDd{IHjyd{BjgSCHR1\QJIF{e2G7LDDFR|UxRTF2LkVOwG0> M2TjNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NES1N|I5Lz5{MkS0OVMzQDxxYU6=
CHO M3m1TmZ2dmO2aX;uJIF{e2G7 NFL1SIhMcSC4YXz1[ZMh\m:{IIPv[Il2dSCobIXvdoV{[2WrbjCoNVAhfU1rIIXweIFs\SCrbjDPRXRROUJ|LYTyZY5{\mWldHXkJGNJVyClZXzsd{whU2l;Mj65Ou69VQ>? M{jkeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUexOFE2Lz5{M{W3NVQyPTxxYU6=
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LNCAP NEfJelVHfW6ldHnvckBie3OjeR?= MkD6Nk42KHWP NYTEWm53OjRiaILz MX;Jcohq[mm2aX;uJI9nKEiGQVO2M2hUWDlyIHnuJIh2dWGwIFzOR2FRKGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKEGUIIDyc5RmcW5ibHX2[Ywh[XRiMj61JJVOKGGodHXyJFI1KGi{czDifUBY\XO2ZYLuJIJtd3RibXX0bI9l MkHUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2NU[xNVMoRjJ7NEW2NVE{RC:jPh?=
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MDA-MB-231/beta41 NVHCe4NlTXO2cn;n[Y5q[yCjc4PhfS=> NHLablAyQCCqcoO= MV;Fd5Rzd2enbnnjJIFkfGm4aYT5JIF1KEWUYnX0ZUApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCqdX3hckBOTEFvTVKtNlMyN2KndHG0NUBk\WyuczDh[pRmeiBzODDodpMh[nlicnXubYxt[SCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;MD6wNFI1|ryP M4nJVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkSxNlA3Lz5{OU[0NVIxPjxxYU6=
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HEK293 MXjGeY5kfGmxbjDhd5NigQ>? Mor1OFghcHK| NFu5WZlC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGVTNWKndHGgeJJidnOoZXP0[YQhcW5iSFXLNlk{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4xOTl7Nd88US=> NX;OVHI2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDRxJ{7DbGVOSkx:L3G+
HEK293 NHGwXIJHfW6ldHnvckBie3OjeR?= MXK0PEBpenN? M1nXNWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSXIu[WyyaHGgeJJidnOoZXP0[YQhcW5iSFXLNlk{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE42ODFzOd88US=> MnLNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQwLz6FaFXNRmw9N2F-
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HL60 M{fPXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIX4bZlIem:5dHigbY5pcWKrdHnvckBw\iCKb33vJJNieGmnboOgLIh2dWGwKTDIUFYxKGOnbHzzJIJ6KE2WVDDhd5NigSxiR1m9OE45Os7:TR?= MoTzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFQwLz6FaFXNRmw9N2F-
SGC7901 Moq5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3ruXWdzd3e2aDDpcohq[mm2aX;uJI9nKEixbX:gd4FxcWWwczCobJVu[W5rIGPHR|c6ODFiY3XscJMh[nliTWTUJIF{e2G7LDDHTV02Njd6zszN M2PBOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1Nyd-Q3jFUWJNRC:jPh?=
HL60 NXrOZYRVTnWwY4Tpc44h[XO|YYm= M3TVVGNwdXCndHn0bZZmKGmwaHnibZRqd25ib3[gS2xWXDFiaX6gbJVu[W5iSFy2NEBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iMj3bNUwzNTOKXXTlc5h6NURvZ3z1Z49{\SC3cIThb4UhdWWjc4Xy[YQh\m:{IEOwJJNm[3NiYomg[I92[mynIILlZ4lxem:lYXygdIxwfCCjbnHsfZNqeyxiS3m9O:69VQ>? M37Lc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ1Nyd-Q3jFUWJNRC:jPh?=
CHO M2HUZWZ2dmO2aX;uJIF{e2G7 MmTmR49ueGW2aYTpeoUhcW6qaXLpeIlwdiCxZjDHUHVVOSBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIEKtX|EtOi1|SG3k[Y95gS2GLXfseYNwe2VidYD0ZYtmKG2nYYP1doVlKG[xcjCzNEB{\WO|IHL5JIRwfWKuZTDy[YNqeHKxY3HsJJBtd3RiYX7hcJl{cXNuIFvpQVfPxE1? NH;4Nng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PC9pPlPoSW1DVDxxYU6=
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
DNMT-1 / DNMT-3a / DNMT-3b ; 

PubMed: 25931004     


Western blot analysis of the effect of physiological concentrations of genistein (0.5–10 µmol/L) on protein expression of the three DNMT subtypes, DNMT1, DNMT3a and DNMT3b in PCa cells.

p-AKT / AKT ; 

PubMed: 27574003     


Genistein inhibits Akt phosphorylation. MCF-7-C3 and T47D cells were treated with genistein at indicated concentrations for 16 h. Phospho-Akt and Akt were detected with western blotting. *P<0.05.

CIP2A / PARP / Cleaved PARP / Caspase-3 / Cleaved Caspase-3 ; 

PubMed: 27574003     


MCF-7, MCF-7-C3 and T47D cells were treated with genistein (GENI) at indicated concentrations for 48 h. Protein levels of CIP2A and PARP in all three cell lines, and caspase-3, cleaved caspase-3 (C-caspase-3) in MCF-7-C3 and T47D cells were detected with western blot analysis.

Foxo3; 

PubMed: 21639915     


Genistein inhibits EGF-induced translocation of FOXO3. Immunofluorescent staining of experimental monolayers revealed that EGF-induced FOXO3 translocation from the nucleus to the cytosol was inhibited by genistein. This experiment was repeated two times in triplicate.

p-ERK; 

PubMed: 18384126     


Genistein dose-dependently inhibits the phosphorylation of ERK1/2. Cells were treated as above and harvested at day 5 after 5 min exposure to adipogenic cocktail, and whole cell protein were subjected to western blotting analysis.

PPARγ / C/EBPα / αP2 ; 

PubMed: 18384126     


Genistein dose-dependently inhibits adipogenic gene expression. Mouse BMSC cultures were exposed to adipogenic cocktail in the presence of increasing concentrations of genistein. Cells were harvested at day 21, and whole cell proteins were subjected to western blotting analysis. 

25931004 27574003 21639915 18384126
Immunofluorescence
ER-β / pS87 ER-β ; 

PubMed: 25931004     


Cells were treated with DMSO (vehicle) or genistein 1 µmol/L and 10 µmol/L for 4hr. Then, cells were fixed, immunostained with pan ER-β and pS87 antibodies simultaneously and visualized by the confocal microscope. The red fluorescent staining in the first column is for pan ER-β, the green fluorescent staining in the second column is for pS87 and the third column is for both images merged together using image J software.

25931004
In vivo Genistein has chemopreventive effects on breast, prostate, and other endocrine-dependent tumors in adult animals. Genistein in the diet reduced the incidence of poorly differentiated prostatic adenocarcinomas in a dose-dependent manner and down-regulated androgen receptor, estrogen receptor-alpha, progesterone receptor, epidermal growth factor receptor, insulin-like growth factor-I, and extracellular signal-regulated kinase-1 but not estrogen receptor-beta and transforming growth factor-alpha mRNA expressions. Dietary genistein protects against mammary and prostate cancers by regulating specific sex steroid receptors and growth factor signaling pathways.[4] Genistein combined with prostate tumor irradiation causes greater inhibition of primary tumor growth and increases control of spontaneous metastasis to para-aortic lymph nodes, increasing mouse survival. Paradoxically, treatment with genistein alone increases metastasis to lymph nodes. [5]

Protocol

Solubility (25°C)

In vitro DMSO 54 mg/mL (199.82 mM)
Water Insoluble
Ethanol ''2 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 270.24
Formula

C15H10O5

CAS No. 446-72-0
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC(=CC=C1C2=COC3=CC(=CC(=C3C2=O)O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01982578 Unknown status Dietary Supplement: Genistein|Other: Placebo Alzheimer''s Disease Fundación para la Investigación del Hospital Clínico de Valencia|University of Valencia September 1 2017 Not Applicable
NCT02499861 Completed Drug: Decitabine and Genistein Cancer St. Justine''s Hospital July 2015 Phase 1|Phase 2
NCT01628471 Completed Drug: decitabine in combination with genistein Non Small Cell Lung Cancer Uman Pharma|DSM Nutritional Products Inc.|MDEIE Ministry Québec Government|INRS-Institut Armand Frappier Université du Québec November 2012 Phase 1|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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EGFR Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID