Genistein (NPI 031L)

Catalog No.S1342

For research use only.

Genistein (NPI 031L), a phytoestrogen found in soy products, is a highly specific inhibitor of protein tyrosine kinase (PTK) which blocks the mitogenic effect mediated by EGF on NIH-3T3 cells with IC50 of 12μM or by insulin with IC50 of 19 μM.

Genistein (NPI 031L) Chemical Structure

CAS No. 446-72-0

Selleck's Genistein (NPI 031L) has been cited by 24 publications

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Biological Activity

Description Genistein (NPI 031L), a phytoestrogen found in soy products, is a highly specific inhibitor of protein tyrosine kinase (PTK) which blocks the mitogenic effect mediated by EGF on NIH-3T3 cells with IC50 of 12μM or by insulin with IC50 of 19 μM.
Targets
EGFR [1] topo II [1]
In vitro

Genistein is an ATP competitive inhibitor. Genistein inhibits tyrosine phosphorylation in isolated enzyme and receptor preparations and in whole cells including platelets, lymphocytes and a variety of cultured cells. It also inhibits EGF-stimulated phosphorylation in cultured cells as well as inhibition of Topo II (topoisomerase II). Genistein inhibits EGF-stimulated tyrosine phosphorylation in cultured A431 epidermoid carcinoma cells. Inhibition is competitive with ATP and noncompetitive with substrate. [1] Genistein blocks the mitogenic effect mediated by EGF, insulin and thrombin on NIH-3T3 cells.[2] Genistein also acts as an agonist at the GPR30 receptor and binds to PPARγ and estrogen receptors. Genistein also binds to PPARγ, acting as an agonist at this receptor with Ki of 5.7 μM. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BxPC3 M4q5cnBzd2yrZnXyZZRqd25iYYPzZZk> M3SzPWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQojQR|Mh[2WubDDsbY5mKGK7IF3UWEBie3OjeTygTWM2OD1|MDFOwG0> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjd6OUezO{c,OTZ5OEm3N|c9N2F-
BT20 NVTlbII6WHKxbHnm[ZJifGmxbjDhd5NigQ>? NHqwdGZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFLUNlAh[2WubDDsbY5mKGK7IF3UWEBie3OjeTygTWM2OD12NjFOwG0> NETjWXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNke4PVc{Pyd-MU[3PFk4Ozd:L3G+
ANN-1 MmrHR5l1d3SxeHnjJIF{e2G7 NI\rVXVEgXSxdH;4bYMh\W[oZXP0JI9vKHZvYXLsJJRz[W6|Zn;ycYVlKG23cnnu[UBCVk5vMTDj[YxteyxiSVO1NF05|ryP NYXBTVhnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOEKwNVYxOyd-OEKwNVYxOzxxYU6=
breast carcinoma cells MmTaR5l1d3SxeHnjJIF{e2G7 NIjUdJZEgXSxdH;4bYMh\W[oZXP0JI9vKE2FRj23JIh2dWGwIHLy[YF{fCClYYLjbY5wdWFiY3XscJMtKEmFNUC9NVUvOc7:TR?= MofqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDJyMU[wN{c,QDJyMU[wN|ww[T5?
3T3 NHrJdZVEgXSxdH;4bYMh[XO|YYm= MV3DfZRwfG:6aXOg[YZn\WO2IH;uJFNVOyClZXzsd{whUUN3ME2yOO69VQ>? NIH5NmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96MkCxOlA{Lz56MkCxOlA{RC:jPh?=
colon cells MnK3R5l1d3SxeHnjJIF{e2G7 M3jHPGN6fG:2b4jpZ{Bm\m[nY4Sgc44hX2mGcjDoeY1idiClb3zvckBk\WyuczygTWM2OD1{Nz63{txO MlnHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDJyMU[wN{c,QDJyMU[wN|ww[T5?
Hepa1clc7 NGPVSmlEgXSxdH;4bYNqfHliYYPzZZk> MoXDO|IhcHK| M4rBSWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGhmeGFzY3zjO{Bk\WyuczDh[pRmeiB5MjDodpMtKEmFNUC9NVHPxE1? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB5NUe0Nkc,OTByN{W3OFI9N2F-
MCF7 MVXGeY5kfGmxbjDhd5NigQ>? MnLPOkBpenN? MmDOTY5pcWKrdHnvckBw\iCyaH;yZo9tKGW|dHXyMYlv\HWlZXSgc5JvcXSqaX7lJIRm[2G{Yn;4fYxie2ViaX6gbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB4IHjyd{whUUN3ME2yOu69VQ>? MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB5NUe0Nkc,OTByN{W3OFI9N2F-
3T3/A31 NYS1d5VOS3m2b4TvfIlkcXS7IHHzd4F6 M{\0T|EhfG9iMUCwJJVoN22O NXvpSW1FPzJiaILz NVTp[4lDS3m2b4TvfIlkcXS7IHHnZYlve3RiQlHMRk9kKG2xdYPlJINtd26nZDCzWFMwSTNzIHPlcIx{KGG2IEGgeI8hOTByIIXnM41NKGGodHXyJFczKGi{czDifUBvcWe{b4PpckBie3OjeR?= MmmwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByOU[4OlMoRjFyMEm2PFY{RC:jPh?=
SH-SY5Y MWXGeY5kfGmxbjDhd5NigQ>? NH74[5dC\2:waYP0JIFkfGm4aYT5JIlvKHS{YX7zZ5JqeHSrb37hcEBi[3SrdnH0bY9vKGG|c3H5JIlvKFOKLWPZOXkhdmW3cn;icIF{fG:vYTDj[YxteyCneIDy[ZN{cW6pIFXzeJJw\2WwIILlZ4VxfG:{IHLleIEtKEWFNUC9NE4xODF5zszN NXrhdpJkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG5NFYzQDBpPkGxPVA3OjhyPD;hQi=>
HeLa MoD5SpVv[3Srb36gZZN{[Xl? NVfiTFR3SWO2aY\heIlwdiCxZjDld5Rzd2enbjDy[ZNxd26|ZTDlcIVu\W62IHnuJGhmVGFiY3XscJMhe3SjYnz5JJRz[W6|ZnXjeIVlKHerdHigbJVu[W5iRYP0do9o\W5icnXj[ZB1d3JiYnX0ZU4tKEWFNUC9NE4xODRzzszN MmLIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF7ME[yPFAoRjFzOUC2NlgxRC:jPh?=
HeLa NHH3[XpHfW6ldHnvckBie3OjeR?= MoCwRYN1cX[jdHnvckBw\iCnc4Tyc4dmdiC{ZYPwc45{\SCnbHXt[Y51KGmwIFjlUIEh[2WubIOgd5Ri[my7IITyZY5{\mWldHXkJJdqfGhiaIXtZY4hTXO2cn;n[Y4hemWlZYD0c5Ih[WyyaHGuMEBGSzVyPUCuNFQ5|ryP MoLWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF7ME[yPFAoRjFzOUC2NlgxRC:jPh?=
Ishikawa MVrFd5Rzd2enbnnjJIF{e2G7 NXrUdoZ6PCCmYYnz M3vr[WV{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hUXOqaXvhe4Eh[2WubIOgZZN{\XO|ZXSgZZMhcW6mdXP0bY9vKG:oIHHsb4FtcW6nIIDoc5NxcGG2YYPlJIFkfGm4aYT5JIFnfGW{IESg[IF6eyCkeTDwZZJiNW6rdILvdIhmdm:uIILlcIVie2ViYYPzZZktKEmFNUC9NE42Oc7:TR?= MoDTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ3MEKzNFcoRjF{NUCyN|A4RC:jPh?=
Hepa-1c1c7 MlTFSpVv[3Srb36gZZN{[Xl? MnixTY5lfWO2aX;uJI9nKE6DRGDIPpF2cW6xbnWgdoVlfWO2YYPlJIlvKG2xdYPlJGhmeGFvMXOxZ|ch[2WubIOgZZN{\XO|ZXSgZZMh\HK3ZzDs[ZZmdCC{ZYH1bZJm\CC2bzDkc5VjdGViZX76fY1mKGGldHn2bZR6KGK7IF3UWEBie3OjeTygR2Q:OjJwOd88US=> MlnkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR3MUC1PVAoRjF2NUGwOVkxRC:jPh?=
Hepa-1c1c7 MVXGeY5kfGmxbjDhd5NigQ>? M3W4TWlvcGmkaYTpc44hd2ZibX;1d4UhUGWyYT2xZ|FkPyClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVA:PDVwMt88US=> NVfHdGJ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS1NVA2QTBpPkG0OVExPTlyPD;hQi=>
T47D NYnI[Go2TXO2cn;n[Y5q[yCjc4PhfS=> NWLwVYZmTXO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCWNEfEJINmdGy|IHHzd4V{e2WmIHHzJIRzfWdibHX2[Ywh[2G3c3nu[{B{fGmvdXzheIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36g[ZF2cX[jbHXueEB1dyBzMDDwUUBme3S{YXTpc4wh[nliYXzhcYFzKGKudXWgZZN{[XluIFHjeIl3cXS7PUCuNFAy|ryP NW\ScJdmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3PFc1OzZpPkG1O|g4PDN4PD;hQi=>
MCF7 NWiyVJR7TXO2cn;n[Y5q[yCjc4PhfS=> M1;q[mV{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegd5RqdXWuYYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:wIHXxeYl3[WynboSgeI8hOTBicF2g[ZN1emGmaX;sJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gT1yLkCwN:69VQ>? Ml3CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5OEe0N|YoRjF3N{i3OFM3RC:jPh?=
T47D M4G0UWV{fHKxZ3XubYMh[XO|YYm= NHvrOWNGe3S{b3flcolkKGGldHn2bZR6KGmwIHj1cYFvKFR2N1SgZ4VtdHNiYYPz[ZN{\WRiYYOg[JJ2\yCuZY\lcEBk[XW|aX7nJJN1cW23bHH0bY9vKG:oIHPlcIwheHKxbHnm[ZJifGmxbjDldZVqfmGuZX70JJRwKDFyMDDwUUBme3S{YXTpc4wh[nliYXzhcYFzKGKudXWgZZN{[XluIFHjeIl3cXS7PUCuNFA6|ryP MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd6N{SzOkc,OTV5OEe0N|Y9N2F-
MCF7 NHy4XndGe3S{b3flcolkKGG|c3H5 MlTqSZN1em:pZX7pZ{Bi[3Srdnn0fUBqdiCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IHTyeYchdGW4ZXygZ4F2e2mwZzDzeIlufWyjdHnvckBw\iClZXzsJJBzd2yrZnXyZZRqd25iZYH1bZZidGWwdDD0c{AyODBicF2g[ZN1emGmaX;sJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gT1yLkCxN:69VQ>? NEPGNIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe4O|Q{Pid-MUW3PFc1OzZ:L3G+
293T NW\2XJF4TnWwY4Tpc44h[XO|YYm= MV3EbZNxdGGlZX3lcpQhd2ZiW{PIYVE4[mW2YT3ld5Rz[WSrb3yg[pJwdSC{ZXPvcYJqdmGwdDDoeY1idiCHUnLleIEh\XiycnXzd4VlKGmwIEK5N3Qh[2WubIOsJGlEPTB;MD6wNFE{|ryP MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJzOUS2N{c,OTZ{MUm0OlM9N2F-
293T MU\GeY5kfGmxbjDhd5NigQ>? NGqzUGFFcXOybHHj[Y1mdnRib3[gX|NJZTF5YnX0ZU1me3S{YXTpc4wh\nKxbTDy[YNwdWKrbnHueEBpfW2jbjDFVoFteGijIHX4dJJme3OnZDDpckAzQTOWIHPlcIx{NCCLQ{WwQVAvODJ7NN88US=> NVHXeoNtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[yNVk1PjNpPkG2NlE6PDZ|PD;hQi=>
Vero Mo\tR5l1d3SxeHnjbZR6KGG|c3H5 MUfDfZRwfG:6aXPpeJkh[WejaX7zeEBi\nKrY3HuJIdz\WWwIH3vcotmgSCYZYLvJINmdGy|LDDJR|UxRTN{LkpOwG0> M4nNSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NESxNFY3Lz5zNkS0NVA3PjxxYU6=
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RAW 264 Mk\MSpVv[3Srb36gZZN{[Xl? NUHyVVd[OjRiaILz NEfpT|lKdmirYnn0bY9vKG:oIEGgeYcwdWxiTGDTMZN1cW23bHH0[YQhXE6IYXzwbIEh[WOldX31cIF1cW:wIHnuJHJCXyB{NkSgZ4VtdHNiYX\0[ZIhOjRiaILzMEBKSzVyPUG4MlHPxE1? M1fTS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{KwNlQ3Lz5zN{OyNFI1PjxxYU6=
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HUVE12 NGDD[JdHfW6ldHnvckBie3OjeR?= NFPVZoNT\X[ncoPhcEBw\iCqeXTyc4dmdiCyZYLvfIll\S2rbnT1Z4VlKEyGSDDhZ5Rqfmm2eTDpckBpfW2jbjDIWXZGOTJiY3XscJM> MkexQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyNki5PFAoRjF6ME[4PVgxRC:jPh?=
HL60 MX\BcpRqdGW3a3XtbYMh[XO|YYm= MV2yOEBpenN? MX7BcpRqdGW3a3XtbYMh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDjcI9vd2enbnnjJIF{e2G7LDDMR|UxRTZwM988US=> MnvzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThzNkO1PFkoRjF6MU[zOVg6RC:jPh?=
L1210 MYrBcpRqdGW3a3XtbYMh[XO|YYm= NH;CRVQzPCCqcoO= NV\1bYFySW62aXzleYtmdWmlIHHjeIl3cXS7IHHnZYlve3RibX;1d4UhVDF{MUCgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KGOub37v[4VvcWNiYYPzZZktKEyFNUC9Ok42|ryP M{Dzc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MU[zOVg6Lz5zOEG2N|U5QTxxYU6=
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HL60 MkfCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYnDVJpJPDhiaILz M{LQWmdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhNPjBiY3XscJMh[W[2ZYKgOFghcHK|LDDJS|UxRTF6LkNOwG0> NYLhPI5GRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUixOlM2QDlpPkG4NVY{PTh7PD;hQi=>
L1210 M12xUmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWmyOEBpenN? NILJRnNIem:5dHigbY5pcWKrdHnvckBw\iCvb4Xz[UBNOTJzMDDj[YxteyCjZoTldkAzPCCqcoOsJGlIPTB;MUiuOe69VQ>? M2TDW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MU[zOVg6Lz5zOEG2N|U5QTxxYU6=
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NCI-ADR-RES MYDBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? Mm[zOFghcHK| MnXwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDld5Rzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iTlPJMWFFWi2URWOgZ4VtdHNiaX7jeYJifGWmIIfpeIghOC5yNjDuUUBw\iCnc4TyZYRqd2xiYX7kJFAvODdibl2gc4YhfGW|dH;zeIVzd26nIHHmeIVzKDR6IHjyd{BjgSC|dXzmc5Jpd2SjbXnu[UBDKGG|c3H5JIlvKHC{ZYPlcoNmKG:oIH\leIFtKGKxdnnu[UB{\XK3bTDhcoQhVnWVZYL1cUwhT0l3ME2xOk46QDJ2zszN MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
MCF12A MWnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MkDFOFghcHK| NYnpOWFbSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBme3S{b3flckBz\WOncITvdk1l\W[rY3nlcpQhcHWvYX6gUWNHOTKDIHPlcIx{KGmwY4XiZZRm\CC5aYToJFAvODZibl2gc4Yh\XO2cnHkbY9tKGGwZDCwMlA4KG6PIH;mJJRme3Sxc4Tldo9v\SCjZoTldkA1QCCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTDpckBxemW|ZX7j[UBw\iCoZYThcEBjd3[rbnWgd4VzfW1iYX7kJG52W2W{dX2sJGdKPTB;MkGuPFc4Ps7:TR?= M173SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlc2Lz5{MUWxN|I4PTxxYU6=
NCI-ADR-RES NYP0S4FDSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M2nVTVQ5KGi{cx?= M{O5O2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[ZN1em:pZX6gdoVk\XC2b4Kt[IVncWOrZX70JIh2dWGwIF7DTU1CTFJvUlXTJINmdGy|IHnuZ5Vj[XSnZDD3bZRpKDFyMDDuUUBw\iCnc4TyZYRqd2xiYX7kJFAvODdibl2gc4YhfGW|dH;zeIVzd26nIHHmeIVzKDR6IHjyd{BjgSC|dXzmc5Jpd2SjbXnu[UBDKGG|c3H5JIlvKHC{ZYPlcoNmKG:oIH\leIFtKGKxdnnu[UB{\XK3bTDhcoQhVnWVZYL1cUwhT0l3ME2yOk46OTV2zszN NYi0T2hNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzPzVpPkKxOVE{Ojd3PD;hQi=>
MCF12A NUPITlk{SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NYryRmUzPDhiaILz NGnWNYJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIV{fHKxZ3XuJJJm[2WydH;yMYRm\mmlaXXueEBpfW2jbjDNR2YyOkFiY3XscJMhcW6ldXLheIVlKHerdHigNVAxKG6PIH;mJIV{fHKjZHnvcEBidmRiMD6wO{BvVSCxZjD0[ZN1d3O2ZYLvcoUh[W[2ZYKgOFghcHK|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XliaX6gdJJme2WwY3Wgc4Yh\mW2YXygZo93cW6nIIPldpVuKGGwZDDOeXNmenWvLDDHTVUxRTNyLkG5PVXPxE1? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
NCI-ADR-RES NIrRe2lCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= Mn;BOFghcHK| MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCQQ1mtRWRTNVKHUzDj[YxteyCrbnP1ZoF1\WRid3n0bEAxNjByMzDuUUBw\iCnc4TyZYRqd2xiYX7kJFAvODFibl2gc4YhfGW|dH;zeIVzd26nIHHmeIVzKDR6IHjyd{BjgSC|dXzmc5Jpd2SjbXnu[UBDKGG|c3H5JIlvKHC{ZYPlcoNmKG:oIEWlJIZmfGGuIHLveolv\SC|ZYL1cUwhT0l3ME2zNk4{PTl2zszN NYjs[mxwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzPzVpPkKxOVE{Ojd3PD;hQi=>
MCF12A NXmzN2M6SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MlH2OFghcHK| NFXHN25CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIV{fHKxZ3XuJJJm[2WydH;yMYRm\mmlaXXueEBpfW2jbjDNR2YyOkFiY3XscJMhcW6ldXLheIVlKHerdHigNE4xODNibl2gc4Yh\XO2cnHkbY9tKGGwZDCwMlAyKG6PIH;mJJRme3Sxc4Tldo9v\SCjZoTldkA1QCCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTDpckBxemW|ZX7j[UBw\iB3JTDm[ZRidCCkb4\pcoUhe2W{dX2sJGdKPTB;M{KuN|U6PM7:TR?= NIDpbGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxN|I4PSd-MkG1NVMzPzV:L3G+
LNCAP M3u4NmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MUK0PEBpenN? NGPwRoJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzOR2FRKGOnbHzzJIV5eHKnc4Ppcoch[W6mcn;n[Y4hemWlZYD0c5IhcW6ldXLheIVlKHerdHigNE43KG6PIH;mJIV{fHKjZHnvcEBidmRiMD63JI5OKG:oIITld5Rwe3Sncn;u[UBi\nSncjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiR1m1NF0{QC57MES1{txO MlzXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUOyO|UoRjJzNUGzNlc2RC:jPh?=
PC3 MlXsRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MkXtOFghcHK| MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHHu[JJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCSQ{OgZ4VtdHNiaX7jeYJifGWmIIfpeIghOC54IH7NJI9nKGW|dILh[IlwdCCjbnSgNE44KG6PIH;mJJRme3Sxc4Tldo9v\SCjZoTldkA1QCCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTygS2k2OD12Mj62OVc6|ryP NEPpS5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxN|I4PSd-MkG1NVMzPzV:L3G+
MCF7 NIfWfXdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MWO0PEBpenN? M1XM[mFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDlfJBz\XO|aX7nJIV{fHKxZ3XuJJJm[2WydH;yJIlv[3WkYYTl[EB4cXSqIECuNFYhdk1ib3[g[ZN1emGmaX;sJIFv\CByLkC3JI5OKG:oIITld5Rwe3Sncn;u[UBi\nSncjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSCrbjDwdoV{\W6lZTDv[kBn\XSjbDDic5ZqdmVic3XyeY0h[W6mIF71V4VzfW1uIFfJOVA:PDVwN{C4PO69VQ>? NEPRR2k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxN|I4PSd-MkG1NVMzPzV:L3G+
FRT NHnLd2NHfW6ldHnvckBie3OjeR?= NYHoZ4hnOjRiaILz Ml33Rolv\GmwZzDh[oZqdmm2eTD0c{BETlSUIF[1NFgh\GWuZYTpc44hdXW2YX70JIV5eHKnc4Pl[EBqdiCob4Lzb49tcW5vc4TpcZVt[XSnZDDGVnQh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaX;kbY5mKGmwZnz1fEBu\WG|dYLl[EBieyC\RmCgdZVmdmOqaX7nJJJifGViYX\0[ZIhOjRiaILzJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiS3S9PU41|ryP NGrqeWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUW2PFMzOyd-MkG1Olg{OjN:L3G+
MCF7 MkXIRY51cWW|dILv[4VvcWNiYYPzZZk> M4XsXFEhfG9iMkCgeW0> NHXwZlk1QCCqcoO= MUfBcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCHUj3wc5NqfGm4ZTDNR2Y4KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gNVdj\XSjIHXzeJJi\GmxbD3pcoR2[2WmIIPlZ5JmfGWmIHHsb4FtcW6nIIDoc5NxcGG2YYPlJIFkfGm4aYT5JIF1KDFidH:gNlAhfU1iYX\0[ZIhPDhiaILzJIJ6KHCqb4PwbIEudGmpaISgdoVxd3K2ZYKgZ4hmdWmudX3pcoV{[2WwY3WgZZN{[Xl? MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOThyMEi1PUc,OjF6MEC4OVk9N2F-
Jurkat T Ml7CRZBweHSxc3nzJIF{e2G7 NYHjWXVxOzZiaILz M1vvS2lv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iSoXyb4F1KFRiY3XscJMhd3[ncnX4dJJme3OrbnegUoVwKGGodHXyJFM3KGi{czDifUBndG:5IHP5eI9u\XS{eR?= NFHW[FM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkG5O|M6Oyd-MkKxPVc{QTN:L3G+
FRT NYDjc2RsTnWwY4Tpc44h[XO|YYm= Ml3hVI91\W62aXH0bY9vKGGldHn2bZR6KGG2IHj1cYFvKEOIVGKg[IVtfGFiRkWwPEBufXSjboStcYVlcWG2ZXSgbY9lcW6nIH\seZgh\XiycnXzd4VlKGmwIILheEBHWlRiY3XscJMh[2:neIDy[ZN{cW6pIH\seY9z\XOlZX70JJBzd3SnaX6gXWZRNUhzNEjRM|EyPTKOIHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfUwhTUN3ME23{txO Mnu1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{OEGxPFUoRjJ{MkixNVg2RC:jPh?=
Human Huh7.5.1 MoSyRY51cX[rcnHsJIF{e2G7 M4D2blczKGi{cx?= MlnORY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGNXKEqISD2xJGo{QTmHTTDpcoZm[3SnZDDpckBJfW2jbjDIeYg4NjVwMTDj[YxteyCjc4Pld5Nm\CCjczDzeZBxemW|c3nvckBw\iC4aYLhcEBz\XCuaXPheIlwdiCjZoTldkA4OiCqcoOgZpkhTUeIUDDhd5NigSxiRVO1NF0yPC52zszN M{PF[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NES1N|I5Lz5{MkS0OVMzQDxxYU6=
CHO NGrTN4NHfW6ldHnvckBie3OjeR?= NXT0U2RGU2lidnHseYV{KG[xcjDzc4RqfW1iZnz1c5Jme2OnaX6gLFExKHWPKTD1dJRic2ViaX6gU2FVWDGEMz30doFve2[nY4Tl[EBEUE9iY3XscJMtKEurPUKuPVbPxE1? M{fZdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUexOFE2Lz5{M{W3NVQyPTxxYU6=
CHO MnflSpVv[3Srb36gZZN{[Xl? NG\TXVVxUUN3MDD2ZYx2\XNiZn;yJJNw\Gm3bTDmcJVwemW|Y3XpckApOTBidV2pJJVxfGGtZTDpckBQSVSSMVKzMZRz[W6|ZnXjeIVlKEOKTzDj[YxteyxiSVO1NF0{Njh7MES1{txO NGi2Z4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W3NVQyPSd-MkO1O|E1OTV:L3G+
CHO NEjSSm1HfW6ldHnvckBie3OjeR?= MYDLbUB3[Wy3ZYOg[o9zKHOxZHn1cUBndHWxcnXzZ4VqdiBqMUCgeW0qKHWydHHr[UBqdiCRQWTQNWIyNXS{YX7z[oVkfGWmIFPIU{Bk\WyuczygT4k:Pc7:TR?= MnS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3N{G0NVUoRjJ|NUexOFE2RC:jPh?=
CHO MkLxSpVv[3Srb36gZZN{[Xl? NETiV|FxUUN3MDD2ZYx2\XNiZn;yJJNw\Gm3bTDmcJVwemW|Y3XpckApOTBidV2pJJVxfGGtZTDpckBQSVSSMVKxMZRz[W6|ZnXjeIVlKEOKTzDj[YxteyxiSVO1NF06NjF{MEGx{txO NYjhVHVtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1O|E1OTVpPkKzOVcyPDF3PD;hQi=>
HuH7 MnviRY51cXCuYYPtc4Rq[WxiYYPzZZk> M1vNdVI1KGi{cx?= NYPJ[GJ6SW62aYDsZZNud2SrYXygZYN1cX[rdImgZYdicW6|dDDsbZZmeiC|cH;yc5pwcXSnIIP0ZYdmKG:oIGDsZZNud2SrdX2gZoVz\2inaTDBUmtCKGW6cILld5NqdmdiR1\QJIlv\mWldHXkJIlvKGi3bXHuJGh2UDdiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBx[XKjc3n0[UBl\X[nbH;wcYVvfCCjZoTldkAzPCCqcoOgZpkheVKWLWDDVkBidmGueYPpd{whUUN3ME2yNO69VQ>? Mn3wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMkW4OFkoRjJ2MUK1PFQ6RC:jPh?=
SKBR3 M4rydWNmdGxiY4njcIUh[XO|YYm= NX\JcplqPTBidH:gO|UhfU1? M2\hclM3KGi{cx?= NGTufpJE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBUU0KUMzDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckBi[2O3bYXsZZRqd25iYYSgS|AwTzFicHjhd4Uh[XRiNUCgeI8hPzVidV2gZYZ1\XJiM{[gbJJ{KGK7IIDyc5Bq\Gm3bTDpc4Rq\GVic4ThbY5qdmdvYnHz[YQh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nz NU\6TpV7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OVYxODRpPkK0OFU3ODB2PD;hQi=>
A431 MWPD[YxtKGO7Y3zlJIF{e2G7 M3HSNlUxKHSxIEe1JJVO NVXkOI9DOzZiaILz M13Mc2NmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJGE1OzFiY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6gZYNkfW23bHH0bY9vKGG2IFewM2cyKHCqYYPlJIF1KDVyIITvJFc2KHWPIHHmeIVzKDN4IHjyd{BjgSCycn;wbYRqfW1iaX;kbYRmKHO2YXnubY5oNWKjc3XkJIZtd3diY4n0c41mfHKrYzDhcoFtgXOrcx?= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR3NkCwOEc,OjR2NU[wNFQ9N2F-
A431 MknnR4VtdCCleXPs[UBie3OjeR?= MWq1NEB1dyB5NTD1US=> MkftN|YhcHK| NFzJ[ZJE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBCPDNzIHPlcIx{KGG|c3Xzd4VlKGG|IHHjZ5VufWyjdHnvckBifCCJMj;NJJBp[XOnIHH0JFUxKHSxIEe1JJVOKGGodHXyJFM3KGi{czDifUBxem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nMYJie2WmIH\sc5ch[3m2b33leJJq[yCjbnHsfZNqeyC{ZXzheIl3\SC2bzDjc451em:u MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR3NkCwOEc,OjR2NU[wNFQ9N2F-
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3T3L1 M3m2bWZ2dmO2aX;uJIF{e2G7 NXv4[opUUW6qaXLpeIlwdiCxZjDHUHVVPCCrbjDtc5V{\SB|VEPMNUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iaX7zeYxqdi2|dHnteYxifGWmIEKt[IVwgHlvRD3bNVREZWeudXPvd4UhfXC2YXvlMEBKSzVyPUKw{txO MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OE8oRkOqRV3CUFww[T5?
Assay
Methods Test Index PMID
Western blot PPARγ / C/EBPα / αP2 ; p-ERK ; Foxo3 ; CIP2A / PARP / Cleaved PARP / Caspase-3 / Cleaved Caspase-3 ; p-AKT / AKT ; DNMT-1 / DNMT-3a / DNMT-3b 18384126 21639915 27574003 25931004
Immunofluorescence ER-β / pS87 ER-β 25931004
In vivo Genistein has chemopreventive effects on breast, prostate, and other endocrine-dependent tumors in adult animals. Genistein in the diet reduced the incidence of poorly differentiated prostatic adenocarcinomas in a dose-dependent manner and down-regulated androgen receptor, estrogen receptor-alpha, progesterone receptor, epidermal growth factor receptor, insulin-like growth factor-I, and extracellular signal-regulated kinase-1 but not estrogen receptor-beta and transforming growth factor-alpha mRNA expressions. Dietary genistein protects against mammary and prostate cancers by regulating specific sex steroid receptors and growth factor signaling pathways.[4] Genistein combined with prostate tumor irradiation causes greater inhibition of primary tumor growth and increases control of spontaneous metastasis to para-aortic lymph nodes, increasing mouse survival. Paradoxically, treatment with genistein alone increases metastasis to lymph nodes. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 54 mg/mL
(199.82 mM)
Water Insoluble
Ethanol ''2 mg/mL

Chemical Information

Molecular Weight 270.24
Formula

C15H10O5

CAS No. 446-72-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CC=C1C2=COC3=CC(=CC(=C3C2=O)O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05073523 Recruiting Other: Diet Healthy Chalmers University of Technology September 27 2021 Not Applicable
NCT01982578 Completed Dietary Supplement: Genistein|Other: Placebo Alzheimer''s Disease Fundación para la Investigación del Hospital Clínico de Valencia|University of Valencia September 1 2017 Not Applicable
NCT02499861 Completed Drug: Decitabine and Genistein Cancer St. Justine''s Hospital July 2015 Phase 1|Phase 2
NCT01628471 Completed Drug: decitabine in combination with genistein Non Small Cell Lung Cancer Uman Pharma|DSM Nutritional Products Inc.|MDEIE Ministry Québec Government|INRS-Institut Armand Frappier Université du Québec November 2012 Phase 1|Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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