Onametostat (JNJ-64619178)

For research use only.

Catalog No.S8624

1 publication

Onametostat (JNJ-64619178) Chemical Structure

CAS No. 2086772-26-9

Onametostat (JNJ-64619178) is a PRMT5 inhibitor with high selectivity and potency (subnanomolar range, PRMT5-MEP-50 IC50=0.14 nM) under different in vitro and cellular conditions, paired with favorable pharmacokinetics and safety properties.

Selleck's Onametostat (JNJ-64619178) has been cited by 1 publication

Purity & Quality Control

Choose Selective Histone Methyltransferase Inhibitors

Biological Activity

Description Onametostat (JNJ-64619178) is a PRMT5 inhibitor with high selectivity and potency (subnanomolar range, PRMT5-MEP-50 IC50=0.14 nM) under different in vitro and cellular conditions, paired with favorable pharmacokinetics and safety properties.
PRMT5 [1]
0.14 nM
In vitro

JNJ-64619178 binds into the SAM binding pocket and reaches the substrate binding pocket to inhibit PRMT5/MEP50 function in a time-dependent manner. Broad cell line panel profiling of JNJ-64619178 reveals a wide range of sensitivity, which is indicative of a genomic dependency instead of a general cytotoxic on-target consequence of PRMT5 inhibition[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Sf9 NU\Ibo1QTnWwY4Tpc44h[XO|YYm= MYfJcohq[mm2aX;uJI9nKG[3bHytcIVv\3SqIHj1cYFvKE5vdHXycYlv[WxiRlzBS{11[WepZXSgVHJOXDVxZoXscEBt\W6pdHigUk11\XKvaX7hcEBJcXN4LYTh[4dm\CCPRWC1NEApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3czDpcoZm[3SnZDDT[lkhcW6|ZXP0JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBw\iCVLXHk[Y5we3muLVytbI9ud2O7c4TlbY5mKG[xcn3heIlwdiC3c3nu[{BpfW2jbjDy[YNwdWKrbnHueEAtKEmFNUC9NE4xODBzM988US=> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODl3NkCxNUc,OzB7NU[wNVE9N2F-
Sf9 MVfGeY5kfGmxbjDhd5NigQ>? NXLUem04OTJyIH3pcpM> MmexTY5pcWKrdHnvckBw\iCodXzsMYxmdme2aDDoeY1idiCQLYTldo1qdmGuIF\MRWcufGGpZ3XkJHBTVVR3IHX4dJJme3OnZDDpckBU\jliaX7z[YN1KGOnbHzzJJV{cW6pIHjpd5RwdmViSELBJIF{KHCncITp[IUh[W[2ZYKgNVIxKG2rboOgbY4heHKnc3XuZ4Uhd2ZiU1HNJIJ6KGirZ3igeIhzd3WpaID1eEBu[XO|IIPw[YN1em:vZYTldkBie3OjeTygTWM2OD1yLkCwNFE1|ryP MmfXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB|Nk[2NVcoRjNyM{[2OlE4RC:jPh?=
A549 M37ER2Z2dmO2aX;uJIF{e2G7 NGTrc5k1QCCqcoO= M1vkSWlvcGmkaYTpc44hd2ZiUGLNWFUhcW5iaIXtZY4hSTV2OTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4he0SPQTDwdo9lfWO2aX;uJIFnfGW{IES4JIhzeyCkeTDIc4VkcHO2IGP0ZYlvN0iFUzDD[YxtVWG|azDE[YVxKFKnZDDTeIFqdiCkYYPl[EBqdW23bn;obZN1d2OqZX3pd5RzgSCvZYToc4QtKEmFNUC9NE4xODB{Nd88US=> NFfEemo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEm1OlAyOSd-M{C5OVYxOTF:L3G+
HEK293 M1K4NGZ2dmO2aX;uJIF{e2G7 MnLCOlAhdWmwcx?= MWLJcohq[mm2aX;uJI9nKGi3bXHuJG4ufGW{bXnuZYwhTkyDRz30ZYdo\WRiUGLNWFUhMDJidH:g[Y5lMSCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KHW|aX7nJIJqd3SrbonsZZRm\CCKMlGgZZMhe3Wkc4TyZZRmKG2nYYP1doVlKGGodHXyJFYxKG2rboOgbY4heHKnc3XuZ4Uhd2ZiU1HNJIJ6KGirZ3igeIhzd3WpaID1eEBu[XO|IIPw[YN1em:vZYTldkBie3OjeTygTWM2OD1yLkCwOlPPxE1? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTh5MEK1PEc,Ojl6N{CyOVg9N2F-

... Click to View More Cell Line Experimental Data

In vivo JNJ-64619178, dosed orally (10 mg/kg, every day), shows selective and efficient blockage of the methylation of SMD1/3 proteins, which are crucial components of the spliceosome and substrates of PRMT5/MEP50. JNJ-64619178 also demonstrates tumor regression in a biomarker-driven human small cell lung cancer xenograft model (NCI-H1048) and prolongs tumor growth inhibition after dosing cessation. In rodent and nonrodent toxicology studies, a tolerated dose of JNJ-64619178 has been identified, with the observed toxicity consistent with on-target activity. In summary, JNJ-64619178 has a favorable preclinical package that supports clinical testing in patients diagnosed with lung cancer and hematologic malignancies1[1].


Solubility (25°C)

In vitro DMSO 100 mg/mL (206.88 mM)
Ethanol '-1 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 483.36


CAS No. 2086772-26-9
Storage powder
in solvent
Synonyms N/A
Smiles C1C(C(C(C1N2C=CC3=C(N=CN=C32)N)O)O)CCC4=CC5=NC(=C(C=C5C=C4)Br)N

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03573310 Active not recruiting Drug: JNJ-64619178 Neoplasms|Solid Tumor Adult|Non-Hodgkin Lymphoma|Myelodysplastic Syndromes Janssen Research & Development LLC July 13 2018 Phase 1

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Histone Methyltransferase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID