HLCL-61 HCL

Catalog No.S8209 Synonyms: HLCL61

For research use only.

HLCL-61 hydrochloride is a potent and selective PRMT5 inhibitor for the treatment of acute myeloid leukemia.

HLCL-61 HCL Chemical Structure

CAS No. 1158279-20-9

Selleck's HLCL-61 HCL has been cited by 2 Publications

Purity & Quality Control

Choose Selective Histone Methyltransferase Inhibitors

Biological Activity

Description HLCL-61 hydrochloride is a potent and selective PRMT5 inhibitor for the treatment of acute myeloid leukemia.
Targets
PRMT5 [1]
(In THP-1 cells)
16.74 μM
In vitro

HLCL-61 shows no inhibitory activity against the type I (PRMT1 and PRMT4) and type II (PRMT7) PRMT family members. HLCL-61 shows effective inhibition of symmetric arginine dimethylation (me2) of histones H3 and H4 in AML samples.Treatment of AML cell lines (MV4-11 and THP-1) and primary blasts with HLCL-61 also results in a decrease of cell viability. HLCL-61 is also effective in promoting apoptosis in MV4-11 and THP-1 cells after 48 h[1].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: AML cell lines
  • Concentrations: 25,50uM
  • Incubation Time: 48h
  • Method: CD11b expression is tested by flow cytometry on cells treated with DMSO or HLCL-61 (25 and 50 μM) for 48 h.

Solubility (25°C)

In vitro

DMSO 76 mg/mL
(199.52 mM)
Ethanol 76 mg/mL
(199.52 mM)
Water Insoluble

Chemical Information

Molecular Weight 380.91
Formula

C23H24N2O.HCl

CAS No. 1158279-20-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCN1C2=C(C=C(C=C2)CNCC3=CC=CC=C3OC)C4=CC=CC=C41.Cl

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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