MS049

Catalog No.S8147

For research use only.

MS049 is a potent and selective inhibitor of PRMT4 and PRMT6 with IC50s of 34 nM and 43 nM, respectively.

MS049 Chemical Structure

CAS No. 2095432-59-8

Selleck's MS049 has been cited by 2 Publications

Purity & Quality Control

Choose Selective Histone Methyltransferase Inhibitors

Biological Activity

Description MS049 is a potent and selective inhibitor of PRMT4 and PRMT6 with IC50s of 34 nM and 43 nM, respectively.
Targets
PRMT4 [1]
(Cell-free assay)
PRMT6 [1]
(Cell-free assay)
34 nM 43 nM
In vitro

MS049 does not inhibit any of the methyltransferases at 50 μM or demethylases at 10 μM and does not display appreciable binding to any of the methyllysine/methylarginine reader proteins using the DSF assay at 200 μM. MS049 demonstrated excellent selectivity over a broad range of epigenetic and nonepigenetic targets. In cellular assays, MS049 potently inhibits the methyltransferase activity of PRMT4 and PRMT6 and reduces levels of Med12me2a and H3R2me2a in HEK293 cells[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: HEK293 cells
  • Concentrations: 0, 1, 10, and 50 μM
  • Incubation Time: 96 h
  • Method:

    HEK293 cells grown in DMEM supplemented with 10% FBS, penicillin (100 U/mL), and streptomycin (100 μg/mL) were seeded on 96-well plates at density 3000/well and treated with compounds at 0, 1, 10, and 50 μM concentrations for 96 h. The confluency was measured using IncuCyte ZOOM live cell imaging device and analyzed with IncuCyte ZOOM software based on phase contrast images.

Solubility (25°C)

In vitro

DMSO 56 mg/mL
(174.29 mM)
Water 56 mg/mL
(174.29 mM)
Ethanol 50 mg/mL
(155.62 mM)

Chemical Information

Molecular Weight 321.29
Formula

C15H26Cl2N2O

CAS No. 2095432-59-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CNCCN1CCC(CC1)OCC2=CC=CC=C2.Cl.Cl

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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