Chloroquine diphosphate

Catalog No.S4157

Chloroquine diphosphate Chemical Structure

Molecular Weight(MW): 515.86

Chloroquine diphosphate is a 4-aminoquinoline anti-malarial and anti-rheumatoid agent, also acting as an ATM activator.

Size Price Stock Quantity  
USD 97 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 17 Publications

5 Customer Reviews

  • NCI-H929 EV and miR-137 OE cells were treated with ATM activator Chloroquine Phosphate (CQ), specific ATM inhibitor KU-55933, and ATR inhibitor AZ20 for 12 hr. Immunoblotting showed the expression of p-ATM, p-Chk2, p-BRCA1, p-ATR and p-Chk1.

    Leukemia, 2017, 31(5):1123-1135. Chloroquine diphosphate purchased from Selleck.

    Western blot showing expression of autophagy-related proteins in four pancreatic cancer cell lines treated with gemcitabine alone or chloroquine alone or both

    Cancer Lett, 2018, 436:129-138. Chloroquine diphosphate purchased from Selleck.

  • Cells were pre-treated with rapamycin (Rapa, 1 μM) for 12 h, or KRIBB3 (KR, 0.5 μM) or chloroquine (CQ, 10 μM) for 2 h, followed by treatment with of palmitate (PA, 150 μM) for 24 h. Then the lipid in L02 cells was observed with Oil Red O (ORO) staining and ORO absorbance detection. Values are means ± SD (n = 3), *p b 0.05, versus matched controls.

    Cell Signal, 2016, 28(8):1086-98. . Chloroquine diphosphate purchased from Selleck.

    Migration assays in T24 and 5637 cells performed after HBSS treatment for 6 hours with or without autophagy inhibitors (CQ and 3MA). Cells cultured in complete medium served as a control. Control versus HBSS, HBSS+CQ, HBSS+3MA; HBSS vs HBSS+CQ, HBSS+3MA. *P < 0.05, **P < 0.01.

    J Cell Biochem, 2018, doi:10.1002/jcb.27788. Chloroquine diphosphate purchased from Selleck.

  • Rapamycin aggravates the autophagic reaction induced by CVB3 infection. HeLa cells were treated with 10 nM rapamycin and chloroquine phosphate (CQ group) for 2 h. The cells were washed thrice and infected with CVB3. In the CVB3 group, HeLa cells were incubated with DMEM containing 10% FBS for 2 h and infected with CVB3. The sham group was incubated with DMEM containing 10% FBS. Two hours later, the medium was refreshed with DMEM containing 2% FBS. At 24 h.p.i., cells were collected for western blot analysis. The LC3-II/LC3-I ratio was increased in the CVB3 and rapamycin group (p<0.01), and p-mTOR was decreased in the CVB3 group (p<0.05) and rapamycin group (p<0.01) compared with the sham group. The changes in the LC3-II/LC3-I ratio and p-mTOR were more obvious in the Rap group compared with the CVB3 group (p<0.05). The LC3-II/LC3-I ratio and p62 was increased in the CQ group compared with the CVB3 group (p<0.05). *p<0.05, compared with the sham group; **p<0.01, compared with the sham group; #p<0.05, compared with the CVB3 group. CVB3, coxsackievirus B3; DMEM, Dulbecco's modified Eagle's medium; FBS, fetal bovine serum; h.p.i., hours post-inoculation; LC3, light chain 3; mTOR, mammalian target of rapamycin.

    Int J Mol Med, 2017, 40(1):182-192. Chloroquine diphosphate purchased from Selleck.

Purity & Quality Control

Choose Selective ATM/ATR Inhibitors

Biological Activity

Description Chloroquine diphosphate is a 4-aminoquinoline anti-malarial and anti-rheumatoid agent, also acting as an ATM activator.
Targets
ATM [1]
(Cell-free assay)
In vitro

Chloroquine is a chemotherapeutic agent for the clinical treatment of malaria. Chloroquine is able to bind to DNA, and inhibit DNA replication and RNA synthesis which in turn results in cell death. The effect of Chloroquine may also be related to the formation of a toxic heme-Chloroquine complex. Chloroquine inhibits trophozoite hemoglobin degradation through increasing vacuolar pH and inhibiting the activity of vacuolar phospholipase, vacuolar proteases, and heme polymerase[1]. Chloroquine possesses definite antirheumatic properties. Chloroquine has immuno-modulatory effects, suppressing the production/release of tumour necrosis factor and interleukin 6. Moreover, Chloroquine exerts direct antiviral effects, inhibiting pH-dependent steps of the replication of several viruses including members of the flaviviruses, retroviruses, and coronaviruses. Its best-studied effects are those against HIV replication[2]. Chloroquine can accumulate inside the macrophage phagolysosome by ion trapping where it exerts potent antifungal activity against Histoplasma capsulatum and Cryptococcus neoformans by distinct mechanisms. Chloroquine inhibits growth of H. capsulatum by pH-dependent iron deprivation, whereas it is directly toxic to C. neoformans[3].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human K562 cells MYjDfZRwfG:6aXRCpIF{e2G7 M4W5S2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGs2PjJiY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxRTNzLkizJI5O MoLZNVg2Ozh3Nke=
human KB cells M2O3OmN6fG:2b4jpZ:Kh[XO|YYm= Mm\HR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2Ih[2WubIOgZpkhdWmlcn;wcIF1\SCvZYToc4QtKEmFNUC9NE43KM7:TR?= MUGxOFY1ODV{NB?=
human MDA-MB-468 cells NG\2XWtEgXSxdH;4bYPDqGG|c3H5 M{XKTFQ5KGh? M1fp[GN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj20Olgh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IGPSRkBie3OjeTDpckBxemW|ZX7j[UBw\iB|LkWgeW0hPC1qMj3jbIxwem9vMUDIMZBp\W6xeHH6bY4uOTBveXypMW4tVi2maXX0bJlt[nW2YX6tNU1idWmwZTDofYRzd2OqbH;ybYRmNCCJSUWwQVIvQDFizszN NEP6WVQyQTl2NUG5Oy=>
human MCF7 cells MYXDfZRwfG:6aXRCpIF{e2G7 NYGxU|hCPDhiaB?= NWrrSnpHS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJFAvPCC3TTCxMUgyNSh2LTi3MZBp\W67bD2xTE1qdWmmYYrvX|QtPS2pXYH1bY5wgGGuaX6tOk16dCmkZX76fYwqeGmyZYLp[IlvNTRveXypMVFJNWKnborvX4RecW2rZHH6c4wuOih|SDmtc45mNCCJSUWwQVUvPTFizszN NVTQe3lmOTl7NEWxPVc>
human MDA-MB-231 cells MYLDfZRwfG:6aXRCpIF{e2G7 M1i2W|Q5KGh? MVrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XliaX6gdJJme2WwY3Wgc4YhOy53IIXNJFQuMDJvY3jsc5JwNTFySD3wbIVvd3ijennuMVExNXmuKT3OMG4u\GmndHj5cIJ2fGGwLUGtZY1qdmViaInkdo9kcGyxcnnk[UwhT0l3ME22MlA5KM7:TR?= M3fYUlE6QTR3MUm3
human HeLa cells Mn20R5l1d3SxeHnjxsBie3OjeR?= MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIF{e2W|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGK7IF3UWEBie3OjeTygTWM2OD1|MDFOwG0> MWKyOFM2PDN{Mh?=
human HepG2 cells NV:wfY5HS3m2b4TvfIlkyqCjc4PhfS=> NITLeGQ1QCCq MV;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUO3MlY5KM7:TR?= Mnf0NlM5OTVzOE[=

... Click to View More Cell Line Experimental Data

Protocol

Solubility (25°C)

In vitro Water 100 mg/mL (193.85 mM)
DMSO Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 515.86
Formula

C18H26ClN3.2H3O4P

CAS No. 50-63-5
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02432417 Not yet recruiting Glioblastoma|Astrocytoma Grade IV Maastricht Radiation Oncology January 2020 Phase 2
NCT02432417 Not yet recruiting Glioblastoma|Astrocytoma Grade IV Maastricht Radiation Oncology January 2020 Phase 2
NCT03503058 Not yet recruiting Malaria Sanaria Inc.|Eijkman Oxford Clinical Research Unit Eijkman Institute|Indonesia University|Congressionally Directed Medical Research Programs March 2019 Phase 2
NCT03503058 Not yet recruiting Malaria Sanaria Inc.|Eijkman Oxford Clinical Research Unit Eijkman Institute|Indonesia University|Congressionally Directed Medical Research Programs March 2019 Phase 2
NCT03636152 Recruiting Chronic Kidney Disease|Cardiovascular Disease|Inflammation|Atherosclerosis|Aortic Stiffness VA Office of Research and Development December 14 2018 Phase 2
NCT03636152 Recruiting Chronic Kidney Disease|Cardiovascular Disease|Inflammation|Atherosclerosis|Aortic Stiffness VA Office of Research and Development December 14 2018 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

ATM/ATR Signaling Pathway Map

Related ATM/ATR Products0

Tags: buy Chloroquine diphosphate | Chloroquine diphosphate ic50 | Chloroquine diphosphate price | Chloroquine diphosphate cost | Chloroquine diphosphate solubility dmso | Chloroquine diphosphate purchase | Chloroquine diphosphate manufacturer | Chloroquine diphosphate research buy | Chloroquine diphosphate order | Chloroquine diphosphate mouse | Chloroquine diphosphate chemical structure | Chloroquine diphosphate mw | Chloroquine diphosphate molecular weight | Chloroquine diphosphate datasheet | Chloroquine diphosphate supplier | Chloroquine diphosphate in vitro | Chloroquine diphosphate cell line | Chloroquine diphosphate concentration | Chloroquine diphosphate nmr
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID