Chloroquine diphosphate

For research use only.

Catalog No.S4157

90 publications

Chloroquine diphosphate Chemical Structure

Molecular Weight(MW): 515.86

Chloroquine diphosphate is a 4-aminoquinoline anti-malarial and anti-rheumatoid agent, also acting as an ATM activator. Chloroquine is also an inhibitor of toll-like receptors (TLRs).

Size Price Stock Quantity  
USD 97 In stock
USD 797 In stock
Bulk Discount
Bulk Inquiry

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Chloroquine diphosphate has been cited by 90 publications

Purity & Quality Control

Choose Selective ATM/ATR Inhibitors

Biological Activity

Description Chloroquine diphosphate is a 4-aminoquinoline anti-malarial and anti-rheumatoid agent, also acting as an ATM activator. Chloroquine is also an inhibitor of toll-like receptors (TLRs).
Targets
ATM [1]
(Cell-free assay)
In vitro

Chloroquine is a chemotherapeutic agent for the clinical treatment of malaria. Chloroquine is able to bind to DNA, and inhibit DNA replication and RNA synthesis which in turn results in cell death. The effect of Chloroquine may also be related to the formation of a toxic heme-Chloroquine complex. Chloroquine inhibits trophozoite hemoglobin degradation through increasing vacuolar pH and inhibiting the activity of vacuolar phospholipase, vacuolar proteases, and heme polymerase[1]. Chloroquine possesses definite antirheumatic properties. Chloroquine has immuno-modulatory effects, suppressing the production/release of tumour necrosis factor and interleukin 6. Moreover, Chloroquine exerts direct antiviral effects, inhibiting pH-dependent steps of the replication of several viruses including members of the flaviviruses, retroviruses, and coronaviruses. Its best-studied effects are those against HIV replication[2]. Chloroquine can accumulate inside the macrophage phagolysosome by ion trapping where it exerts potent antifungal activity against Histoplasma capsulatum and Cryptococcus neoformans by distinct mechanisms. Chloroquine inhibits growth of H. capsulatum by pH-dependent iron deprivation, whereas it is directly toxic to C. neoformans[3].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human K562 cells NIewVIdEgXSxdH;4bYPDqGG|c3H5 Mnv6R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVA:OzFwOEOgcm0> NYK0NHVMOTh3M{i1Olc>
human KB cells M1z1O2N6fG:2b4jpZ:Kh[XO|YYm= NEPvTW5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMSiClZXzsd{BjgSCvaXPyc5Bt[XSnIH3leIhw\CxiSVO1NF0xNjZizszN NFPqSGQyPDZ2MEWyOC=>
human MDA-MB-468 cells NYHrN4dQS3m2b4TvfIlkyqCjc4PhfS=> MVW0PEBp MX7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNE[4JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XliaX6gdJJme2WwY3Wgc4YhOy53IIXNJFQuMDJvY3jsc5JwNTFySD3wbIVvd3ijennuMVExNXmuKT3OMG4u\GmndHj5cIJ2fGGwLUGtZY1qdmViaInkdo9kcGyxcnnk[UwhT0l3ME2yMlgyKM7:TR?= M2X0OlE6QTR3MUm3
human MCF7 cells NYXqbW1kS3m2b4TvfIlkyqCjc4PhfS=> M3nXNlQ5KGh? M3TBZ2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUBqdiCycnXz[Y5k\SCxZjCwMlQhfU1iMT2oNU0pPC1qNz3wbIVvgWxvMVitbY1q\GG8b2u0MFUu\12zdXnuc5hidGmwLU[tfYwq[mWweonsLZBqeGW{aXTpck01NXmuKT2xTE1j\W68b2vkYYlucWSjen;sMVIpO0hrLX;u[UwhT0l3ME21MlUyKM7:TR?= MX[xPVk1PTF7Nx?=
human MDA-MB-231 cells NFv2[mhEgXSxdH;4bYPDqGG|c3H5 NVLSUppXPDhiaB?= MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XliaX6gdJJme2WwY3Wgc4YhOy53IIXNJFQuMDJvY3jsc5JwNTFySD3wbIVvd3ijennuMVExNXmuKT3OMG4u\GmndHj5cIJ2fGGwLUGtZY1qdmViaInkdo9kcGyxcnnk[UwhT0l3ME22MlA5KM7:TR?= NXvCTlc1OTl7NEWxPVc>
human HeLa cells MnW3R5l1d3SxeHnjxsBie3OjeR?= Mm\DR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCkeTDNWHQh[XO|YYmsJGlEPTB;M{Cg{txO NHvBSI8zPDN3NEOyNi=>
human HepG2 cells NW\CbYltS3m2b4TvfIlkyqCjc4PhfS=> NWq4VmJWPDhiaB?= MWfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUO3MlY5KM7:TR?= NYPWO2tMOjN6MUWxPFY>

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot
ZEBRA / p-S824 KAP1 ; 

PubMed: 28249048     


HH514-16 BL cells were treated with chloroquine and harvested at different times post-treatment for immunoblotting with antibodies as indicated. 

p62 / LC3 ; 

PubMed: 26677873     


Antiautophagic activity of Lys01. Panc 10.05 cells were treated for 24 h with the indicated doses of chloroquine or Lys01 and were probed by Western blot.

28249048 26677873
Immunofluorescence
p-S824 KAP1 / ZEBRA ; 

PubMed: 28249048     


HH514-16 BL cells were treated with chloroquine (CQ) or chloroquine plus KU-55933 (CQ+KU) and harvested at 24 hours followed by staining with anti-phospho KAP1 (S824) plus anti-ZEBRA antibodies and visualized at 1000X magnification.

CD1d-β2m / LAMP1 ; 

PubMed: 20368272     


HEK293-CD1d WT cells were treated overnight with 20 μM of chloroquine (or vehicle). The cells were then fixed, permeabilized and stained with the anti-CD1d-β2m mAb (red), anti-LAMP-1 (green) and Hoechst (blue). The stained cells were then analyzed by confocal microscopy. 

SQSTM1 / MAP1LC3B ; 

PubMed: 29872540     


Immunofluorescent images of astrocytoma SNB-19 cells treated with DMSO, emetine or chloroquine for 24 h, then stained with SQSTM1 protein (green), MAP1LC3B protein (red) and nuclei (blue). 

28249048 20368272 29872540
Growth inhibition assay
Cell viability ; 

PubMed: 25521075     


A549 cells were treated with serial concentrations of chloroquine for 72 h, and Huh7 cells were treated with the same concentrations for 48 h. Tumour cell viability was determined by cell viability assay. Data represent means from at least three independent experiments ± SD. 

25521075

Protocol

Solubility (25°C)

In vitro Water 100 mg/mL (193.85 mM)
DMSO Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 515.86
Formula

C18H26ClN3.2H3O4P
CAS No. 50-63-5
Storage powder
in solvent
Synonyms N/A
Smiles CCN(CC)CCCC(C)NC1=CC=NC2=C1C=CC(=C2)Cl.O[P](O)(O)=O.O[P](O)(O)=O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04340544 Recruiting Drug: Hydroxychloroquine|Drug: Placebo COVID-19 University Hospital Tuebingen|Robert Bosch Medical Center|Universitätsklinikum Hamburg-Eppendorf|Bernhard Nocht Institute for Tropical Medicine April 22 2020 Phase 3
NCT02378532 Completed Drug: Chloroquine|Radiation: Radiotherapy|Drug: Temozolomide Glioblastoma Multiforme Maastricht Radiation Oncology August 2016 Phase 1
NCT02496741 Completed Drug: Metformin and chloroquine combination Glioma|Cholangiocarcinoma|Chondrosarcoma Academisch Medisch Centrum - Universiteit van Amsterdam (AMC-UvA) November 2015 Phase 1|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
selleck catalog

ATM/ATR Signaling Pathway Map

Related ATM/ATR Products

  • AZD6738(Ceralasertib) New

    AZD6738 is an orally active, and selective ATR kinase inhibitor with IC50 of 1 nM. Phase 1/2.

  • CHIR-99021 (CT99021) New

    CHIR-99021 (CT99021) is a GSK-3α and GSK-3β inhibitor with IC50 of 10 nM and 6.7 nM, respectively. CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3β compared to CDKs. CHIR99021 functions as a Wnt/β-catenin activator and induces autophagy.

  • Dorsomorphin (Compound C) New

    Dorsomorphin is a potent, reversible, selective AMPK inhibitor with Ki of 109 nM in cell-free assays, exhibiting no significant inhibition of several structurally related kinases including ZAPK, SYK, PKCθ, PKA, and JAK3. Dorsomorphin selectively inhibits the BMP type I receptors ALK2, ALK3 and ALK6. Dorsomorphin is used in promoting specific cell differentiation and inducing cancer cell line autophagy.

  • KU-55933 (ATM Kinase Inhibitor)

    KU-55933 (ATM Kinase Inhibitor) is a potent and specific ATM inhibitor with IC50/Ki of 12.9 nM/2.2 nM in cell-free assays, and is highly selective for ATM as compared to DNA-PK, PI3K/PI4K, ATR and mTOR. KU‑55933 (ATM Kinase Inhibitor) inhibits the activation of autophagy‑initiating kinase ULK1 and results in a significant decrease of autophagy.

  • VE-821

    VE-821 is a potent and selective ATP competitive inhibitor of ATR with Ki/IC50 of 13 nM/26 nM in cell-free assays, shows inhibition of H2AX phosphorylation, minimal activity against PIKKs ATM, DNA-PK, mTOR and PI3Kγ.

  • KU-60019

    KU-60019 is an improved analogue of KU-55933, with IC50 of 6.3 nM for ATM in cell-free assays, 270- and 1600-fold more selective for ATM than DNA-PK and ATR,and is a highly effective radiosensitizer.

    Features:Improved analog of KU-55933, and is more effective at blocking ATM-mediated DDR events.

  • Berzosertib (VE-822)

    VE-822 is an ATR inhibitor with IC50 of 19 nM in HT29 cells.

    Features:VE-822 is a highly selective and potent derivative of the ATR inhibitor VE-821.

  • AZ20

    AZ20 is a novel potent and selective inhibitor of ATR kinase with IC50 of 5 nM in a cell-free assay, 8-fold selectivity over mTOR.

    Features:AZ20 is the first reported inhibitor of ATR protein kinase demonstrating tumor growth inhibition in vivo.

  • CP-466722

    CP-466722 is a potent and reversible ATM inhibitor, does not affect ATR and inhibits PI3K or PIKK family members in cells.

  • ETP-46464

    ETP-46464 is a potent and selective inhibitor of ATR with IC50 of 25 nM.

    Features:Selective ATR inhibitor and particularly toxic to p53-deficient cancer cells. Often used as a pharmacologic probe due to its ideal pharmacological properties.

Tags: buy Chloroquine diphosphate | Chloroquine diphosphate ic50 | Chloroquine diphosphate price | Chloroquine diphosphate cost | Chloroquine diphosphate solubility dmso | Chloroquine diphosphate purchase | Chloroquine diphosphate manufacturer | Chloroquine diphosphate research buy | Chloroquine diphosphate order | Chloroquine diphosphate mouse | Chloroquine diphosphate chemical structure | Chloroquine diphosphate mw | Chloroquine diphosphate molecular weight | Chloroquine diphosphate datasheet | Chloroquine diphosphate supplier | Chloroquine diphosphate in vitro | Chloroquine diphosphate cell line | Chloroquine diphosphate concentration | Chloroquine diphosphate nmr
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID