For research use only.

Catalog No.S1570

79 publications

KU-60019 Chemical Structure

CAS No. 925701-49-1

KU-60019 is an improved analogue of KU-55933, with IC50 of 6.3 nM for ATM in cell-free assays, 270- and 1600-fold more selective for ATM than DNA-PK and ATR,and is a highly effective radiosensitizer.

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10mM (1mL in DMSO) USD 195 In stock
USD 150 In stock
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Selleck's KU-60019 has been cited by 79 publications

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Description KU-60019 is an improved analogue of KU-55933, with IC50 of 6.3 nM for ATM in cell-free assays, 270- and 1600-fold more selective for ATM than DNA-PK and ATR,and is a highly effective radiosensitizer.
Features Improved analog of KU-55933, and is more effective at blocking ATM-mediated DDR events.
ATM [1]
(Cell-free assay)
6.3 nM
In vitro

Compared to KU-55933, KU-60019 is an improved more water-soluble inhibitor of the ATM kinase, while displaying similar target selectivity. KU-60019 has little activity against DNA-PKcs and ATR with IC50 values of 1.7 μM and >10 μM, respectively, as well as 229 other protein kinases such as PI3K, mTOR and mTOR/FKBP12. KU-60019 displays 3- to 10-fold more potency than KU-55933 at blocking radiation-induced phosphorylation of key ATM protein targets such as p53, γ-H2AX, and CHK2, in human glioma U87 and U1242 cells, as 1 μM of KU-60019 significantly induces >70% decrease of p53 (S15) phosphorylation to which extent ~10 μM of KU-55933 is required to achieve. KU-60019 effectively radiosensitizes human glioma cells with dose-enhancement ratio of 1.7 and 4.4 at 1 μM and 10 μM, respectively, and also radiosensitizes the normal fibroblasts but not the A-T fibroblasts. KU-60019 treatment (3 μM) blocks basal and insulin-induced AKT S473 phosphorylation by 70% and ~50%, respectively, and completely reduces radiation-induced AKT phosphorylation below the level of control. The effect of KU-60019 on AKT S473 phosphorylation can be seen in glioma cell lines and normal fibroblasts but not in A-T (h-TERT) cells, and can be significantly blocked by phosphatase inhibitor okadaic acid, suggesting a critical role of ATM kinase in regulating AKT phosphorylation via unknown phosphatase. Consistent with the inhibition of prosurvival AKT signaling, KU-60019 at 3 μM significantly inhibits migration and invasion of human glioma U87 cells by >70% and ~60%, respectively, as well as U1242 cells by >50% and ~60% respectively. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BAECs NEnMVnVHfW6ldHnvckBCe3OjeR?= MYWxNOKh|ryP NE\pZoMyyqCqwrC= M2fIeGROW09? MmXnZYJwdGm|aHXzJJRp\SCyaH;zdIhwenmuYYTpc44hd2ZiQWTNMXNmejF7OEG= NVfWOldIOjV2OUi1OFI>
BAECs MY\GeY5kfGmxbjDBd5NigQ>? NGPTeYcyOMLizszN NWjP[WtROcLiaNMg MYnEUXNQ MW\pcohq[mm2czD0bIUhcW6lcnXhd4UhcW5iTl;TJIFkfGm4aYT5 M3fPclI2PDl6NUSy
U87 MXXLbY5ie2ViQYPzZZk> MkXhN{DPxE4EoB?= NHL2cnYxNjViaB?= MUjEUXNQ M{DuR:KhcW6qaXLpeJMhfGinIFHUUUBscW6jc3W= Mn7kNlM3OjB2MEm=
U1242 M4\wZ2Fxd3C2b4Ppd{BCe3OjeR?= MWGzJO69VcLi NVzU[Jp{OcLiaNMg NGX4T5VFVVOR NYLSOFdkemGmaX;z[Y5{cXSrenXzJIh2dWGwIHfsbY9u[SClZXzsdy=> M{DmOVI{PjJyNEC5
U87 NI\mOZBCeG:ydH;zbZMhSXO|YYm= NFzlVJg{KM7:TdMg MWqxxsBpyqB? M1S2W2ROW09? M3TjOJJi\Gmxc3Xud4l1cXqnczDoeY1idiCpbHnvcYEh[2WubIO= NFvT[WczOzZ{MESwPS=>
U1242  MVnLbY5ie2ViQYPzZZk> NV\TU4VlOC5yMT2zJO69VcLi MmfZNUBp NVrFcGFXTE2VTx?= M2HveYJtd2OtczDBWG0hc2mwYYPlJIFkfGm4aYT5JIF1KGyxdzDjc45k\W62cnH0bY9vew>? M3zvXVIzOzdyNEi1

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
p-ATM / ATM / p-AKT / AKT / PKM2 ; 

PubMed: 30799198     

CAFs (Cancer-associated fibroblasts) were cultured in normoxia or hypoxia for 8 h and treated with or without KU60019 (5 μM), protein levels of p-ATM, ATM, p-AKT, AKT and PKM2 were determined by Western blotting.


PubMed: 30799198     

CAFs (Cancer-associated fibroblasts) were cultured in normoxia or hypoxia for 8 h and treated with or without KU60019 (5 μM), the membrane GLUT1 was shown by immunofluorescence staining. Scale bar, 50 μm. 

In vivo In orthotopic glioma U1242/luc-GFP xenograft models, the combination of KU-60019 and radiation significantly increases survival of mice than KU-60019 alone, radiation alone, or no treatment. In addition, p53-mutant gliomas is much more sensitive to KU-60019 radiosensitization than wild-type glioma. [2]


Cell Research:[1]
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  • Cell lines: U87 and U1242
  • Concentrations: Dissolved in water, final concentrations ~3 μM
  • Incubation Time: 1, 3, and 5 days
  • Method: Cells are exposed to KU-60019 for 1, 3, and 5 days. Cell growth is determined by AlamarBlue. AlamarBlue is added to the medium to the recommended final concentration. Plates are incubated for 1 hour at 37 °C, fluorescence is determined on a Fluoro-Count plate reader (excitation 530 nm, emission 590 nm), and values are taken as a measure of cell growth. Cell survival is determined by trypan blue/fluorescence activated cell sorting (FACS) assay.
    (Only for Reference)
Animal Research:[2]
- Collapse
  • Animal Models: Athymic female mice harboring orthotopic glioma U1242/luc-GFP tumors or human glioma U1242/luc-GFP tumors
  • Dosages: KU-60019 (10 μM) is delivered at a rate of 0.5 μL/h by osmotic pump; KU-60019 (250 μM) in 12.5 μL is infused by CED.
  • Administration: Administered intratumorally by convection-enhanced delivery or osmotic pump
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 18 mg/mL warmed (32.86 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 547.67


CAS No. 925701-49-1
Storage powder
in solvent
Synonyms N/A
Smiles CC1CN(CC(O1)C)CC(=O)NC2=CC3=C(C=C2)SC4=C(C3)C=CC=C4C5=CC(=O)C=C(O5)N6CCOCC6

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Frequently Asked Questions

  • Question 1:

    what vehicle do you recommend for in vivo use of this compound?

  • Answer:

    The formula for i.p. injections: 5% stock solution (100mg/ml) +30% PEG 300+ddH2O could reach a final concentration of 5mg/ml.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID