Cabozantinib (BMS-907351)

For research use only.

Catalog No.S1119 Synonyms: XL184

63 publications

Cabozantinib (BMS-907351) Chemical Structure

Molecular Weight(MW): 501.51

Cabozantinib (XL184, BMS-907351) is a potent VEGFR2 inhibitor with IC50 of 0.035 nM and also inhibits c-Met, Ret, Kit, Flt-1/3/4, Tie2, and AXL with IC50 of 1.3 nM, 4 nM, 4.6 nM, 12 nM/11.3 nM/6 nM, 14.3 nM and 7 nM in cell-free assays, respectively. Cabozantinib induces PUMA-dependent apoptosis in colon cancer cells via AKT/GSK-3β/NF-κB signaling pathway.

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Selleck's Cabozantinib (BMS-907351) has been cited by 63 publications

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Choose Selective VEGFR Inhibitors

Biological Activity

Description Cabozantinib (XL184, BMS-907351) is a potent VEGFR2 inhibitor with IC50 of 0.035 nM and also inhibits c-Met, Ret, Kit, Flt-1/3/4, Tie2, and AXL with IC50 of 1.3 nM, 4 nM, 4.6 nM, 12 nM/11.3 nM/6 nM, 14.3 nM and 7 nM in cell-free assays, respectively. Cabozantinib induces PUMA-dependent apoptosis in colon cancer cells via AKT/GSK-3β/NF-κB signaling pathway.
Targets
VEGFR2/KDR [1]
(Cell-free assay)
c-Met [1]
(Cell-free assay)
0.035 nM 1.3 nM
In vitro

XL184 has weak inhibitory activity against RON and PDGFRβ with IC50 of 124 nM and 234 nM, respectivey, and has low activity against FGFR1 with IC50 of 5.294 μM. [1] XL184 at low concentration (0.1-0.5 μM) is sufficient to induce marked inhibition of constitutive and inducible Met phosphorylation and its resultant downstream signaling in MPNST cells, and inhibit HGF-induced MPNST cell migration and invasion. XL184 also induces marked inhibition of Met and VEGFR2 phosphorylation in cytokine-stimulated human umbilical vein endothelial cells (HUVECs). Although XL-184 has no significant effect on MPNST cell growth at 0.1 μM, XL184 at 5-10 μM significantly inhibits the MPNST cell growth. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
E98NT  MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHnoPHoxNjBzLUGwJO69VQ>? M33sTmROW09? NI\l[WVKSzVyPUi5JI5O NUS5N|A5OjN2OESwNFY>
SNU-5  NVTlNJh2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRSBzOTDuUS=> NGn4OVMzOTl{NkG5NS=>
Hs746T  NGLzfYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXBeohKSzVyPUmuPUBvVQ>? NXXmSndtOjF7Mk[xPVE>
SNU-1  Mn\1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mni0TWM2OD13MkKzJI5O NYfrWm84OjF7Mk[xPVE>
SNU-16 M1fMVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFr6R2dKSzVyPUGxOFkhdk1? MWKyNVkzPjF7MR?=
MDA-MB-231 NVK3foNIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFSyXpZKSzVyPTC2OFIyKG6P NVLtXW4{OjF7Mk[xPVE>
U87MG MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVjJR|UxRTF6NUGgcm0> NUfMT|JDOjF7Mk[xPVE>
H441  M13lUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4Xie2lEPTB;MkG3NFAhdk1? MXyyNVkzPjF7MR?=
H69 M3Kyfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvUTWM2OD1{MEKwNEBvVQ>? MViyNVkzPjF7MR?=
PC3 MlfQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\aTWM2OD1zMEiwNEBvVQ>? Mlz0NlE6OjZzOUG=
MTC-TT NEX3clRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\LUW1vUUN3ME2wMlA1KCtiMD6wN{DPxE1? NWjFRpRGOjF2N{C5PVU>
MZ-CRC MkPDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRiB3IN88US=> M4K0fFIyPDdyOUm1
TPC-1 NW\OXnBYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFXFSnBKSzVyPUCuNFYhMyByLkCyJO69VQ>? NUXQT4xKOjF2N{C5PVU>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-MET(Y1234/1235) / MET / p-EGFR(Y1068) / EGFR / p-Gab1(Y627) / Gab1 / p-AKT(S473) / p-ERK / p-EIF4E; 

PubMed: 29188469     


MET kinase inhibitors but not emibetuzumab inhibited MET signaling pathway in Hs746t cells in vitro by western blot analysis. Hs746t cells were treated for 24 h with 133 nM (20 μg/ml) or 667 nM (100 μg/ml) emibetuzumab or MET kinase inhibitors (merestinib, PF04217903, crizotinib, cabozantinib) at 15.6, 62.5, 250 or 1000 nM.

p-MET / p-ERK / p-AKT; 

PubMed: 29520051     


Western blot analysis of cellular pathways modifications after CBZ 5 μM treatment in HOS, MG-63, Saos-2 and U-2 OS cells; (A) CBZ 5 μM inhibits c-MET phosphorilation after 1 hour of treatment; downstream ERK activation kinetic adopts a sigmoidal shape in all OS cell lines, whereas AKT activation is strongly inhibited in HOS, Saos-2 and U-2OS, but not in MG-63 cells.

29188469 29520051
Immunofluorescence
α-tubulin; 

PubMed: 29520051     


Immunostaining of mitotic spindle in control and CBZ 5 μM treated HOS cells using a monoclonal antibody against α-tubulin (in green); white arrows indicate the mitotic spindle poles; control cells show normal bipolar spindles during metaphase formation, whereas treated cells show tripolar spindles during prometaphase (II), metaphase (III) and anaphase (IV); bar scale = 10 μm.

29520051
Growth inhibition assay
Cell viability; 

PubMed: 23661005     


Cells were left untreated or were treated with 5, 7.5 or 10 μM cabozantinib (XL184). Seventy-two hours later viability was detected by MTT-assay and apoptosis by staining with annexinV-FITC followed by FACS-analysis.

23661005
In vivo XL184 treatment at 30 mg/kg in RIP-Tag2 mice with spontaneous pancreatic islet tumors disrupts 83% of the tumor vasculature, reduces pericytes and empty basement membrane sleeves, causes widespread intratumoral hypoxia and extensive tumor cell apoptosis, and slows regrowth of the tumor vasculature after drug withdrawal, more significantly compared with XL999 that blocks VEGFR but not c-Met, leading to only 43% reduction in vascularity, suggesting that concurrent inhibition of VEGFR and other functionally relevant receptor tyrosine kinases (RTK) amplifies angiogenesis inhibition. XL184 also decreases invasiveness of primary tumors and reduces metastasis. [1] XL184 at 30 mg/kg/day significantly abrogates human MPNST xenografts growth and metastasis in SCID mice. [2] Administration of XL184 induces dose-dependent inhibition of tumor growth in breast, lung, and glioma tumor models, in association with decreased tumor and endothelial cell proliferation as well as increased apoptosis. A single oral dose of XL184 is sufficient to induce sustained tumor growth inhibition in MDA-MB-231 tumor-bearing mice and C6 tumor-bearing rats at 100 mg/kg and 10 mg/kg, respectively. [3]

Protocol

Cell Research:

[2]

- Collapse
  • Cell lines: ST88-14, STS26T, and MPNST724
  • Concentrations: Dissolved in DMSO, final concentrations ~10 μM
  • Incubation Time: 48 hours
  • Method:

    Cells are exposed to various concentrations of XL184 for 48 hours. Cell growth is determined by MTS assays using CellTiter96 Aqueous Non-Radioactive Cell Proliferation Assay kit. Absorbance is measured at a wavelength of 490 nm, and the absorbance values of treated cells are presented as a percentage of the absorbance of untreated cells.


    (Only for Reference)
Animal Research:

[1]

- Collapse
  • Animal Models: RIP-Tag2 transgenic mice in a C57BL/6 background with spontaneous pancreatic islet tumors
  • Dosages: ~60 mg/kg
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (199.39 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 501.51
Formula

C28H24FN3O5

CAS No. 849217-68-1
Storage powder
in solvent
Synonyms XL184
Smiles COC1=CC2=C(C=C1OC)C(=CC=N2)OC3=CC=C(NC(=O)C4(CC4)C(=O)NC5=CC=C(F)C=C5)C=C3

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Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04338269 Not yet recruiting Drug: Atezolizumab|Drug: Cabozantinib Carcinoma Renal Cell Hoffmann-La Roche July 31 2020 Phase 3
NCT04091750 Recruiting Drug: Nivolumab|Drug: Ipilimumab|Drug: Cabozantinib Melanoma Georgetown University|MedStar Franklin Square Medical Center|Hackensack Meridian Health|Exelixis March 2 2020 Phase 2
NCT03964337 Suspended Drug: Cabozantinib|Procedure: Radical Prostatectomy Prostate Cancer|Prostate Cancer Adenocarcinoma|Non-Metastatic Duke University|Exelixis March 17 2020 Phase 2
NCT04300140 Not yet recruiting Drug: AVB-S6-500|Drug: Cabozantinib (Cabo) Clear Cell Renal Cell Carcinoma Aravive Inc. March 2020 Phase 1|Phase 2

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID