SU1498

Catalog No.S6535

SU1498 Chemical Structure

Molecular Weight(MW): 390.52

SU1498, a powerful inhibitor of KDR (IC50 = 0.7 μM), stimulates accumulation of phosphorylated ERK1/2 in endothelial cells.

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Biological Activity

Description SU1498, a powerful inhibitor of KDR (IC50 = 0.7 μM), stimulates accumulation of phosphorylated ERK1/2 in endothelial cells.
Targets
KDR [1]
()
In vitro

SU1498 accentuates accumulation of phosphorylated ERK in endothelial cells and inhibits its kinase activity while having no effect on phosphorylation of Akt. SU1498 does not affect phospho-ERK translocation to nucleus[1].

Protocol

Cell Research:

[1]

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  • Cell lines: HUVECs
  • Concentrations: 10 μM
  • Incubation Time: 15 min
  • Method:

    Cells are inoculated into the wells of 6-well culture plates (3 × 105 cells/well) and grown for 16 h in DMEM-based medium at 37 °C in the atmosphere of 5% CO2. After an overnight starvation (0.5% fetal bovine serum), the medium is removed, and the cells are washed with PBS and overlaid with 0.4 ml of DMEM. From this point on, all incubations are done at 25 °C. The inhibitors (SU1498, 10 μM; AG1433, 10 μM; AG1296, 25 μM; PD153035, 1 μM) are added and followed 15 min later by the growth factors: SPP, 1 μm; VEGF, 100 ng/ml; HGF, 100 ng/ml; bFGF, 10 ng/ml; EGF, 10 ng/ml. Forskolin (10 μm), a gratuitous inducer of adenylyl cyclase, is used as a control because of its known ability to enhance ERK phosphorylation. After a 10-min incubation, the medium is siphoned off, and the cells are lysed with 100 μl of lysis buffer. Samples (10 μl) of each lysate are fractionated by SDS-PAGE, and proteins are transferred to nitrocellulose and probed with anti-phospho-Akt or anti-phospho-ERK1/2 antibody. To show comparable loading, anti-actin antibody is used.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 78 mg/mL (199.73 mM)
Ethanol 78 mg/mL (199.73 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 390.52
Formula

C25H30N2O2

CAS No. 168835-82-3
Storage powder
in solvent
Synonyms N/A

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID