Anacardic Acid

Catalog No.S7582 Synonyms: 6-pentadecylsalicylic Acid

For research use only.

Anacardic Acid (6-pentadecylsalicylic Acid) is a potent inhibitor of p300 and p300/CBP-associated factor histone acetyltranferases, which also has antibacterial activity, antimicrobial activity, prostaglandin synthase inhibition, and tyrosinase and lipoxygenase inhibition.

Anacardic Acid Chemical Structure

CAS No. 16611-84-0

Selleck's Anacardic Acid has been cited by 19 publications

Purity & Quality Control

Choose Selective Histone Acetyltransferase Inhibitors

Biological Activity

Description Anacardic Acid (6-pentadecylsalicylic Acid) is a potent inhibitor of p300 and p300/CBP-associated factor histone acetyltranferases, which also has antibacterial activity, antimicrobial activity, prostaglandin synthase inhibition, and tyrosinase and lipoxygenase inhibition.
Targets
Tip60 [2]
(Cell-free assay)
PCAF [1]
(Cell-free assay)
p300/CBP [1]
(Cell-free assay)
5 μM 8.5 μM
In vitro

Anacardic acid is found to be a common inhibitor of several clinically targeted enzymes such as NFkB kinase, histone acetyltransferase (HATs), lipoxygenase (LOX-1), xanthine oxidase, tyrosinase and ureases[4]. Anacardic acid is also an efficient inhibitor of Tip60 in vitro.It inhibits the Tip60-dependent acetylation and activation of the ATM protein kinase in HeLa cells, and sensitizes the cells to the cytotoxic effects of radiation[2]. Anacardic acid can effectively prevent cell damage induced by H2O2 because this can be converted to ROS, hydroxyl radicals, in the presence of metal ions[4].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Hi5 cells NUjGUW5VTnWwY4Tpc44h[XO|YYm= Mk\tUo9vNWOxbYDleIl1cX[nIHnubIljcXSrb36gc4YhcHWvYX6gdoVkd22kaX7hcpQhUGm|LVfTWEB1[WepZXSgWmNRKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EBJcTViY3XscJM> MUOyN|I1PTNzMR?=
HCT116 cells MXnDfZRwfG:6aXPpeJkh[XO|YYm= M3PPTlczKGh? M2jZdmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCjZoTldkA4OiCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxRTBwM{ig{txO MkfMNlMzPDV|MUG=
Hi5 cells M136NmZ2dmO2aX;uJIF{e2G7 M1HwSGlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiSHnzMWdUXCC2YXfn[YQhXkOSIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2eyCrbn\lZ5Rm\CCKaUWgZ4VtdHNiYYPz[ZN{\WRiYYOgRWRRKG[xcn3heIlwdiCrbnP1ZoF1\WRiZn;yJFIxKG2rboOgdJJwcXJidH:gd5Vje3S{YYTlJIFl\Gm2aX;uJI1m[XO3cnXkJIFnfGW{IEmwJI1qdnNiYomgUmFFUCClb4XwcIVlKGG|c3H5MEBKSzVyPUCuNFI1KM7:TR?= NH;SN2wzOzJ2NUOxNS=>
Assay
Methods Test Index PMID
Growth inhibition assay Cell viability 30210681
Western blot GRP78 / Hsp70 / CDK4 ; E-cadherin / Vimentin / MMP-9 ; ErbB2 30210681 24120871
In vivo Anacardic acid attenuates over-expression of Gata4, α-MHC and cTnT in fetal mouse hearts exposed to ethanol. Anacardic acid inhibits binding of P300, PCAF to the promoter of Gata4[3].

Protocol (from reference)

Kinase Assay:[3]
  • HAT and HDAC Activity Assays :

    After homogenization of cardiac tissues, nucleoproteins are extracted using a Nuclear Extract Kit according to the manufacturer's instructions. HAT and HDAC activities of the nuclear protein extracts are determined using a colorimetric assay included in the HAT and HDAC assay kits.

Cell Research:[2]
  • Cell lines: HeLa and 293T cells, SQ20B and SCC35 squamous cell carcinoma cell lines
  • Concentrations: 0-100 μM
  • Incubation Time: 40 min
  • Method: HeLa and 293T cells are grown in Dulbecco’s modified Eagle’s medium/10% fetal calf serum. SQ20B and SCC35 squamous cell carcinoma cell lines are cultured in modified eagles medium/10% fetal calf serum.For anacardic acid (EMD Biosciences, CA) exposure, cells are preincubated with anacardic acid for 40 min, irradiated, then allowed to recover for 4 h. Cells are then switched to fresh media and allowed to grow for 10 days.
Animal Research:[3]
  • Animal Models: 9 to 11 week old Kunming mice
  • Dosages: 0, 1.25, 2.5, 5, 10 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5% DMSO+40%PEG300+5% tween80+50% H2O
For best results, use promptly after mixing.

8mg/mL

Chemical Information

Molecular Weight 348.52
Formula

C22H36O3

CAS No. 16611-84-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCCCCCCCCCCCCCCC1=C(C(=CC=C1)O)C(=O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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