CHIR-99021 (CT99021)

Catalog No.S1263

For research use only.

CHIR-99021 (CT99021) is a GSK-3α and GSK-3β inhibitor with IC50 of 10 nM and 6.7 nM, respectively. CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3β compared to CDKs. CHIR99021 functions as a Wnt/β-catenin activator and induces autophagy.

CHIR-99021 (CT99021) Chemical Structure

CAS No. 252917-06-9

Selleck's CHIR-99021 (CT99021) has been cited by 585 publications

Purity & Quality Control

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Biological Activity

Description CHIR-99021 (CT99021) is a GSK-3α and GSK-3β inhibitor with IC50 of 10 nM and 6.7 nM, respectively. CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3β compared to CDKs. CHIR99021 functions as a Wnt/β-catenin activator and induces autophagy.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LOUCY MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnj1TWM2OD1zOEKuOlg5KM7:TR?= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
SN12C Mn;5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFq5XoNKSzVyPUG4Nk45OjlizszN Mn2yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
A427 Ml;JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFX0fYhKSzVyPUG4OE43OzFizszN NH:2NZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
DOK NHXhZ|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRTF6ND62OFMh|ryP NID6ZYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
MDA-MB-415 NGPRS|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX\K[WFUUUN3ME2xPFQvPjVzIN88US=> MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
MRK-nu-1 NF3oem5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF7n[lRKSzVyPUG4OE46PzJizszN MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
LB2241-RCC MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4qwTGlEPTB;MUi1MlE1PSEQvF2= NUjGN2dsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
NCI-H1563 M2TyPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7UTmNkUUN3ME2xPFUvOjB7IN88US=> NFiybVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
MDA-MB-453 NHTZPJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn3iTWM2OD1zOE[uPVQyKM7:TR?= NVXJN5pqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
MONO-MAC-6 NXjEVmt3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUKwcG9YUUN3ME2xPFcvOzl{IN88US=> NW[5SZo3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
CAPAN-1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M360RWlEPTB;MUi3MlQ2OyEQvF2= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
SK-LU-1 NIjIN3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTF6OD60Nlgh|ryP NY\wNFE2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
NCI-H1299 NWXDfnpXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGDMS2RKSzVyPUG4PE44OTlizszN MnHOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
KG-1 NHvqUpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MojMTWM2OD1zOEmuO|k5KM7:TR?= M32ybFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
ETK-1 M1S3[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTF7MD6xPFEh|ryP MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
NCI-H838 NVS1fFBjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmPLTWM2OD1zOUCuN|Qh|ryP M4P4TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
HCE-4 M4izUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M13YfGlEPTB;MUmwMlk6OiEQvF2= NHL1Om49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
A2780 MlHYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHXGcnZKSzVyPUG5Nk45QTFizszN MlG4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
COLO-668 NF\kZW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTWTWM2OD1zOUOuNVU4KM7:TR?= M1zFOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
HGC-27 M{XYNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXT3RYhxUUN3ME2xPVUvPTN6IN88US=> M4qy[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SW1088 NF;BbFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTF7NT62O|ch|ryP MnHaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
KOSC-2 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGrYXYJKSzVyPUG5OU45OThizszN NGPyfnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
SW780 Ml3NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTF7Nj63N|Qh|ryP NVnPW|VORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
TE-1 NXnCVFNQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYr2VplDUUN3ME2xPVYvOjZizszN NV\r[nRFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
22RV1 NGPoc2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPnVWN1UUN3ME2xPVcvPDJ2IN88US=> Mn7RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
IA-LM MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTF7Nz64NlIh|ryP M4K4OlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
TE-5 MknkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV60flNRUUN3ME2xPVgvODN|IN88US=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
JVM-3 MlSwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn:zTWM2OD1zOUiuOFE6KM7:TR?= M3zQcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
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SJRH30 NWe1bVBYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIDmeIlKSzVyPUKwNE42OTVizszN MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
COLO-679 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;6[mlKSzVyPUKwNE45PTlizszN M1u2R|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
MFM-223 MnrZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIT5NpBKSzVyPUKwNU42QDdizszN NUj6W4JMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
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NCI-H345 NYDpeZlnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MknmTWM2OD1{MEOuNFI4KM7:TR?= NUL1bWY3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
KYSE-70 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4TXTGlEPTB;MkCzMlg4QSEQvF2= NVn6W21pRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
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DJM-1 M4fiVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF34ZplKSzVyPUKwPU45QTdizszN MkDmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
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HT-1080 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnnzTWM2OD1zM{KuNlA1KM7:TR?= NGLFVWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
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OPM-2 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1y0R2lEPTB;MUKzMlE4PiEQvF2= MljGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
HT-29 NGPPUYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUCx[I11UUN3ME2xNlIvPzd6IN88US=> MlHrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
HAL-01 NFW3fVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rtUWlEPTB;MUKyMlQ6QSEQvF2= NVPmVWZpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
KYSE-140 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrURXRmUUN3ME2xNlIvOTRzIN88US=> NH3OXmk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
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NCI-N87 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnzRTWM2OD1zMkGuNFQ1KM7:TR?= NWDhbJZvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
OVCAR-3 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTFzOT65NVUh|ryP M1LKclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
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MKN28 M4L4XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfKO|ZHUUN3ME24Mlg5PTF4IN88US=> M1\FSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
CAL-72 M4DkOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2C3[2lEPTB;OD63OlEyOyEQvF2= Mm\EQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MV-4-11 M1WyfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV21eWZFUUN3ME24MlU1OzB2IN88US=> MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
SW1417 NXT4XGtnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mki1TWM2OD15Lk[0OVg1KM7:TR?= NFPTUms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
G-361 MkPwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYTJR|UxRTdwNE[1OlEh|ryP NVLzZ4t2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
MC116 NHfXVlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTdwMki2PVEh|ryP M4HRVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
RT-112 NEnrdJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTdwMU[0OVYh|ryP M2DYOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
CAL-85-1 MofjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkLCTWM2OD14Lk[wPVM3KM7:TR?= NH;rOJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
BB65-RCC NU\QTGtzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvDdGVKSzVyPU[uOVMyQTVizszN NFu4TWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
CGTH-W-1 NYG3VJd7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{fxO2lEPTB;Nj60NVch|ryP NHXUOXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
WM-115 NY\LeFlJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTVwOEG1NFkh|ryP MmLIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
SK-MEL-2 M4fLUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{DmNGlEPTB;Nj6zPFM1PCEQvF2= M2\J[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
BL-41 MkPSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnixTWM2OD13LkixNlY3KM7:TR?= Mn3TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
LB2518-MEL M3LjSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nQS2lEPTB;NT63NlYzPyEQvF2= NHraWm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
D-392MG NFnSWVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUnCSJBHUUN3ME21MlQ6OTR3IN88US=> NHfoVWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
NCI-H2452 NFvJWHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2r2PWlEPTB;NT60OVYzQCEQvF2= NW\iRoFsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
SW1710 M2XyR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NW\ve3RlUUN3ME21MlQzPDV4IN88US=> NVvlNm41RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
SK-OV-3 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGXKenhKSzVyPUSuPVk{PCEQvF2= NW\SeGlxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
ALL-PO NH:3eY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37BOGlEPTB;ND64N|g2QCEQvF2= M{WxNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
BxPC-3 Ml3mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIix[lNKSzVyPUSuO|A{QTFizszN MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
RCC10RGB MkDmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEH0VGlKSzVyPUSuNlk1PzlizszN MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
NKM-1 MnrFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3wbnBKSzVyPUSuNVM6PzFizszN NU[1N3BMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
MHH-ES-1 NUjUS4l[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGG4VnlKSzVyPUSuNFQ2PDVizszN NWH4O|JQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
OE19 MorjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjqTFQ5UUN3ME2zMlk3PDVzIN88US=> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
TE-15 MkfzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnJc4ZVUUN3ME2zMlk2OzR|IN88US=> MlTTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MOLT-16 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIqyW|FKSzVyPUOuPFg2PTZizszN NFviO5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
A172 MkPQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkHETWM2OD1|Lke1OFk5KM7:TR?= MlK2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
HCC1569 M1XPOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvqTWM2OD1|LkS4N|A2KM7:TR?= NFfrbVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
HOP-62 NWr0ZnB{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTNwM{[wOVQh|ryP MojMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
A704 M4\qfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFS3PGZKSzVyPUOuNFgyOiEQvF2= NYXDSWhORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
SCC-9 NWrsZ4F7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlrwTWM2OD1{Lkm1OFg2KM7:TR?= Ml\qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
COLO-829 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjpWHVbUUN3ME2yMlc3PTRizszN MoeyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
RPMI-7951 NVzOPY1iT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\LTWM2OD1{LkewNVk{KM7:TR?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
SK-LMS-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MluzTWM2OD1{Lk[0NVI5KM7:TR?= NWm4S3pZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
SNU-423 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2Cy[GlEPTB;Mj60PVc5PyEQvF2= NHfK[Wg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
NCI-H2122 NVXOZ2VLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnTTI5KSzVyPUKuNlc3QDRizszN MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
RS4-11 MnnLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{LkSWlEPTB;MT65OVQyPSEQvF2= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
HCC1395 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3;Pd2lEPTB;MT63PFI4OyEQvF2= NIX1cmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
MIA-PaCa-2 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHfycIhKSzVyPUGuO|U6PzdizszN MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
KYSE-180 NX3meodLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYX1WJR[UUN3ME2xMlczPzh6IN88US=> Mn[0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
BCPAP MojoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\o[o5lUUN3ME2xMlY3QDl6IN88US=> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
A498 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkTSTWM2OD1zLkOwOFY6KM7:TR?= M{m1T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
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S-117 MlTzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPBem9KSzVyPUCuPVIyOzFizszN NG\a[o09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
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LOXIMVI M2T1UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlH5TWM2OD1yLkW4PFMzKM7:TR?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
769-P MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkfqTWM2OD1yLkW4OFk6KM7:TR?= NH3ZPFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
GCT MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NICzdoRKSzVyPUCuN|A3PzJizszN M3L1PVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SW982 MnzRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1SydWlEPTB;MD6yNVQyPiEQvF2= Mo\2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
ES-CCE M{fJV2Z2dmO2aX;uJGF{e2G7 NHH1WJc2yqEQvF2= NVnpUVVIOyCm NF7MeWNFVVOR MWDTeJJwdmeueTDpcoR2[2W|IITo[UBi[3Srdnn0fUBw\iCZboSvZoV1[S2lYYTlcolvKHCjdHj3ZZk> NHfUNo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe3PVM3PSd-MkS3O|k{PjV:L3G+
ES-D3 NInHUlNHfW6ldHnvckBCe3OjeR?= M3;OdlXDqM7:TR?= NXrKVopVOyCm M2[1V2ROW09? M4DzTnN1em:wZ3z5JIlv\HWlZYOgeIhmKGGldHn2bZR6KG:oIGfueE9j\XSjLXPheIVvcW5icHH0bJdigQ>? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd5OUO2OUc,OjR5N{mzOlU9N2F-
ES-CCE NH;5d4FEgXSxdH;4bYMhSXO|YYm= MoPhNVDDqM7:TR?= NFSxflY{KGR? MW\EUXNQ NXPxRo9tUW6mdXPld{Bud2SncnH0[UBkgXSxdH;4bYNqfHl? M3Sx[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{e5N|Y2Lz5{NEe3PVM3PTxxYU6=
ES-D3 M4LIRmN6fG:2b4jpZ{BCe3OjeR?= NEm5XpUyOMLizszN NY\NZ2FROyCm Mny2SG1UVw>? MlXETY5lfWOnczDtc4RmemG2ZTDjfZRwfG:6aXPpeJkhf2m2aDDJR|UxKG:oIESuPUDPxE1? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd5OUO2OUc,OjR5N{mzOlU9N2F-
Neural progenitor cells Mkn0SpVv[3Srb36gRZN{[Xl? MoW3N{DPxE1? NUTZeZpFOTBiZB?= MlfBSG1UVw>? M3n5TWlv\HWlZYOg[4Vv\XKjdHnvckBw\iCwZYXyZYwheHKxZ3XubZRweiClZXzsdy=> NYTRS5ZNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N|gxOzRpPkK0OlM5ODN2PD;hQi=>
A549 NWL2O3FoSXCxcITvd4l{KEG|c3H5 NHnzVVA2KML3TR?= MkTCNlQhcA>? MVnEUXNQ MXPJcoNz\WG|ZYOgZ4VtdCCjcH;weI9{cXN? NYPI[ZhYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlc3QDhpPkK0N|Y4Pjh6PD;hQi=>
H157 M4nvdWFxd3C2b4Ppd{BCe3OjeR?= M4HBcFUhyrWP NYnv[lk4OjRiaB?= MYHEUXNQ M2nZbGRw\XNibn;0JJNq\26rZnnjZY51dHliYX\m[YN1KGGyb4D0c5Nqew>? Ml;1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|Nke2PFgoRjJ2M{[3Olg5RC:jPh?=
H460 MXfBdI9xfG:|aYOgRZN{[Xl? MWK1JOK2VQ>? NYnFOY5IOjRiaB?= NVjUOoVMTE2VTx?= M1fyR2F1fGWwdXH0[ZMh[2WubDDhdI9xfG:|aYO= MoD3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|Nke2PFgoRjJ2M{[3Olg5RC:jPh?=
H157 NIHMNWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILaZ3c2KML3TR?= MYeyOEBp MXjEUXNQ M33wdXBzd22xdHXzJINmdGxicILvcIln\XKjdHnvci=> NWTDVWN2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlc3QDhpPkK0N|Y4Pjh6PD;hQi=>
H460 NIT5UXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4nPN|UhyrWP NUjlfVY6OjRiaB?= NUGyPHg6TE2VTx?= NGrZT3lRem:vb4Tld{Bk\WyuIIDyc4xq\mW{YYTpc44> M{SzbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{[3Olg5Lz5{NEO2O|Y5QDxxYU6=
embryonic stem cells (ESC) MX\GeY5kfGmxbjDBd5NigQ>? MWezJOK2VQ>? NYH3XnJ5OTFiZB?= Mlf0SG1UVw>? NVK4VYhvWHKxbX;0[ZMhe2WuZj3y[Y5mf2GuIH;mJI5iy6:4ZT30fZBmKG2xdYPlJIVu[nK7b37pZ{B{fGWvIHPlcIx{KCiHU1Op NVXvXYpQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OFM{OTJpPkKzPFQ{OzF{PD;hQi=>
DN3 MofFSpVv[3Srb36gRZN{[Xl? NHrpZ2IyOCEQvF2= M{XTfFk3KGh? M{LrUGROW09? MoLjTY5kemWjc3XzJINmdGxiZHX2[YxweG2nboSgZY5lKHC{b3zp[oVz[XSrb36= MnTPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3Mk[5PFkoRjJ|NUK2PVg6RC:jPh?=
platelets MonFSpVv[3Srb36gRZN{[Xl? NFjZPZcyOCEQvF2= NInsdXgyPSCvaX6= M4Kxc2ROW09? NFXrfnlKdmO{ZXHz[ZMhfGi{b33ibY4udWWmaXH0[YQheGyjdHXs[ZQh[WepcnXnZZRqd25iYX7kJGFVWCC|ZXPy[ZRqd25? NWHuRVZ[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyN|k5PzdpPkKzNlM6QDd5PD;hQi=>
hiPSC-NPs MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjBN-KBkc7:TR?= NH3GW444OiCq M2W4TmROW09? NUO0OI1yUW6lcnXhd4V{KGOnbHygdJJwdGmoZYLheIlwdsLi NVHYPVhuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFI3QDdpPkKyOlQzPjh5PD;hQi=>
INS-1E M4jZR2Z2dmO2aX;uJGF{e2G7 M2XSfFIvPSEQvF2= NELWNmYzPCCq NX7neGNmTE2VTx?= NXjTPIdLUW6mdXPld{BiKHKnZIXjeIlwdiCxZjDOVFEhd3[ncnX4dJJme3Orb36= NVnTT4t2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0Nlc4ODRpPkKyOFI4PzB2PD;hQi=>
PANC1 M2nUWmFxd3C2b4Ppd{BCe3OjeR?= M4T2VOKhPSEQvF2= MmrRO|IhcA>? MVTEUXNQ NUT0cIZMXHKrZ3fldpMh[W5iYYDvdJRwfGmlIILld5BwdnOn NIXXV|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkKwNVE5Pid-MkKyNFEyQDZ:L3G+
Endothelial cell(EC) MlLBSpVv[3Srb36gRZN{[Xl? NFzV[Y0{KM7:TR?= Ml7wSG1UVw>? MX;Qdo9ud3SnczDlcoRwfGinbHnhcEBk\WyuIDjFR{kh\GmoZnXy[Y51cWG2aX;u Mn6xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyNUKwNFYoRjJ{MEWyNFA3RC:jPh?=
HMC1.2 M1GzOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlyzN|Ah|ryP NGnkZ3c4OiCq Mn\nSG1UVw>? NI\mbllT\WS3Y3XzJINmdGxic4Xyeol3[Wx? NVe1VXdHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwN|k{ODFpPkKyNFM6OzBzPD;hQi=>
Sca-1+ HPC M2P5TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWGyJO69VQ>? MoDQOkBl NHLDcHpFVVOR MVvTfY5memerc4TpZ4FtdHlicILvcY91\XNiU3PhMVEsyqCKUFOgdJJwdGmoZYLheIlwdiCjbnSgZ49td267IH\vdo1ifGmxbjD0c4dmfGincjD3bZRpKEWJRh?= NXLLWIFlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG2NlQ{PDhpPkKxOlI1OzR6PD;hQi=>
Oct4/Sox2/Klf4-infected MEFs MmruSpVv[3Srb36gRZN{[Xl? MYGxNEDPxE1? M4G0RVI5KGR? NYjhcXN2TE2VTx?= NFm0bIVRem:vb4Tld{BOTU[|IILldJJw\3KjbX3pcochXHKjboPkeYNm\CCkeTDPZ5Q1NCCVb4iyMEBidmRiS3zmOC=> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh|OUC1OUc,OTl6M{mwOVU9N2F-
H4IIE MXPGeY5kfGmxbjDBd5NigQ>? NVHnUZFNOTBizszN M3Lle|I1KGh? MWTEUXNQ MWTS[Yd2dGG2ZYOg{tIu[2G2ZX7pckBi[3Srdnn0fUBidmRiVFnSSUBkd262YXnubY5oKHC{b33veIVzKGGldHn2bZR6 NH3rV249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUO1NFE6PSd-MUWzOVAyQTV:L3G+
insulin receptor–expressing CHO-IR MkDLSpVv[3Srb36gRZN{[Xl? MVy1NFAhdk1? NFvrTXg{OCCvaX6= Mn;HSG1UVw>? NIPsbYlC[3SrdnH0[ZMhT2y7Y3;n[Y4hW3mwdHjhd4UhMEeVKR?= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjZyNkS5O{c,OTJ4ME[0PVc9N2F-
3T3-L1 M4jtWGZ2dmO2aX;uJGF{e2G7 NWiyTWRtOyEQvF2= MXS0JIg> NILBN5JFVVOR MUnJcohq[mm2czDwdoVi\Gmyb3P5eIUh\GmoZnXy[Y51cWG2aX;u NUXkcpFLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKwOVUzODBpPkGyNFU2OjByPD;hQi=>
CHO-IR MWPGeY5kfGmxbjDBd5NigQ>? MnjqN|AhdWmw NYPVZ2RQTUN3ME2wMlc4KM7:TR?= M{PSZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MEG2NVIyLz5{OUCxOlEzOTxxYU6=
HepG2 NGH1OHhHfW6ldHnvckBCe3OjeR?= M{j4c|MhcA>? NXXJSXFMTUN3ME2xMlUh|ryP MoLoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{NkGwNlMoRjJ{Mk[xNFI{RC:jPh?=
Assay
Methods Test Index PMID
Western blot Cleaved PARP / CD31 / VE-Cadherin / α-SMA / vimentin ; β-catenin ; CTNNB1 30709379 24165128 23993102
Growth inhibition assay Cell viability 22039301
Immunofluorescence Oct3/4 ; SOX10/EdU 24779365 28174705
In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: Insulin receptor–expressing CHO-IR cells; Primary rat hepatocytes
  • Concentrations: 0.01-10 μM
  • Incubation Time: 30 min
  • Method:

    CHO-IR cells expressing human insulin receptor are grown to 80% confluence in Hamm’s F12 medium with 10% fetal bovine serum and without hypoxanthine. Trypsinized cells are seeded in 6-well plates at 1 × 106 cells/well in 2 ml of medium without fetal bovine serum. After 24 h, medium is replaced with 1 ml of serum-free medium containing GSK-3 inhibitor or control (final DMSO concentration <0.1%) for 30 min at 37°C. Cells are lysed and centrifuged 15 min at 4°C/14000g. The activity ratio of GS is calculated as the GS activity in the absence of glucose-6-phosphate divided by the activity in the presence of 5 mmol/l glucose-6-phosphate, using the filter paper assay of Thomas et al.

  • (Only for Reference)
Animal Research:

[1]

  • Animal Models: Female db/db mice; Male ZDF rats
  • Dosages: 8-48 mg/kg
  • Administration: oral administration
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 10 mg/mL
(21.48 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.

3.5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.34
Formula

C22H18Cl2N8

CAS No. 252917-06-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=CN=C(N1)C2=CN=C(N=C2C3=C(C=C(C=C3)Cl)Cl)NCCNC4=NC=C(C=C4)C#N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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