CHIR-99021 (CT99021)

For research use only.

Catalog No.S1263

454 publications

CHIR-99021 (CT99021) Chemical Structure

CAS No. 252917-06-9

CHIR-99021 (CT99021) is a GSK-3α and GSK-3β inhibitor with IC50 of 10 nM and 6.7 nM, respectively. CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3β compared to CDKs. CHIR99021 functions as a Wnt/β-catenin activator and induces autophagy.

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Selleck's CHIR-99021 (CT99021) has been cited by 454 publications

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Choose Selective GSK-3 Inhibitors

Biological Activity

Description CHIR-99021 (CT99021) is a GSK-3α and GSK-3β inhibitor with IC50 of 10 nM and 6.7 nM, respectively. CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3β compared to CDKs. CHIR99021 functions as a Wnt/β-catenin activator and induces autophagy.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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SN12C NWnj[pVDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXLJR|UxRTF6Mj64Nlkh|ryP Mk\iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
A427 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTF6ND62N|Eh|ryP NV24V5ZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
DOK MnH4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTF6ND62OFMh|ryP MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
MDA-MB-415 MlrMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV2xR|RxUUN3ME2xPFQvPjVzIN88US=> MnWzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MRK-nu-1 NVvB[5FLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVn0bJRmUUN3ME2xPFQvQTd{IN88US=> M4DWOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
LB2241-RCC MmXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3TYfWlEPTB;MUi1MlE1PSEQvF2= MkfTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
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HCE-4 M{DJ[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\ob5hKSzVyPUG5NE46QTJizszN NGnyV|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
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HT-1080 NVq1OXVYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlnTTWM2OD1zM{KuNlA1KM7:TR?= NHzMOZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
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OPM-2 NIe5VXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkH3TWM2OD1zMkOuNVc3KM7:TR?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
HT-29 NU\PXZhGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk[5TWM2OD1zMkKuO|c5KM7:TR?= M4DYUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
HAL-01 NG\rWpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M17kVmlEPTB;MUKyMlQ6QSEQvF2= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
KYSE-140 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M33KcWlEPTB;MUKyMlE1OSEQvF2= NIfGc4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
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NCI-N87 NHXLNJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;wfHVlUUN3ME2xNlEvODR2IN88US=> NGWzT3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
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ST486 M4XsTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;SflhjUUN3ME2xOE4zOjVzIN88US=> MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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SF539 MnnXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF74cJpKSzVyPUG0MlAyOTZizszN M1r2OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SW48 NGDoWmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nLSmlEPTB;MUOuO|I3PSEQvF2= M3zVW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
MEL-HO NU\VZ3RmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4T6e2lEPTB;MUOuPFgzQSEQvF2= M3\tXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
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T98G NFqzWYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkSwTWM2OD1zMz60O|E4KM7:TR?= M{[wXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
DSH1 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\TTmlEPTB;MUOuN|g4QSEQvF2= NYraSIpmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
NCI-H82 MoDwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\KV5FKSzVyPUGzMlI6ODlizszN MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
C8166 NGX5V4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXfMXG9xUUN3ME2xN{4zOzF6IN88US=> MkTSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
PC-14 NI[3dFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInJS3BKSzVyPUGyMlMzPCEQvF2= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
PA-1 NVfKWXR{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;STWM2OD1zMT65N{DPxE1? NHvacVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
HEL MmXaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmfGTWM2OD1zMT62OVc6KM7:TR?= NV7hSZp6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
NCI-H526 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHhUXpKSzVyPUGxMlE{PjVizszN Ml\CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
SNU-387 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXzJR|UxRTFyLkeyN|Uh|ryP NUDVTYdpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
HT-3 M3X0TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLlT|dFUUN3ME2xNE43OTd7IN88US=> M{DyeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
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NCI-H28 NYH2[mZ[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnXSTWM2OD1zMD60OFE{KM7:TR?= NYrpcpFDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
LB1047-RCC MnL4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWfxT2pIUUN3ME25Mlk6Pzl6IN88US=> M4TETVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
U-118-MG MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTlwOUS0OFQh|ryP NYrzd|VkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
DBTRG-05MG NH;2SXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTGTHFLUUN3ME25Mlc4PzdizszN NX7WT5lLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
EFM-19 M3rU[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mln6TWM2OD17Lke1OVY3KM7:TR?= M3\UUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SK-N-DZ MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoC0TWM2OD17Lk[1O|QzKM7:TR?= MkC5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
LB996-RCC MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnfvTWM2OD17LkWyN|k4KM7:TR?= NYOw[nNZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
U-2-OS MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW[3U2VbUUN3ME25MlQ2ODB4IN88US=> NH\6VW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
CTV-1 Mlv5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH:0WnFKSzVyPUmuOFM5QTRizszN Mn\KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
COLO-205 NEXjc45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrHenVKSzVyPUmuOFM5OjFizszN NFHIZnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
NCI-H64 MofKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTlwNEKyOVUh|ryP NFruRms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
NCI-H1755 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYiyPI5qUUN3ME25MlM6QTh4IN88US=> MlizQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
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MEG-01 NF;hS5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXi4dJRGUUN3ME24Mlk6ODR|IN88US=> MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
MKN28 M1HnNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljzTWM2OD16Lki4OVE3KM7:TR?= MljJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
CAL-72 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDDTWM2OD16Lke2NVE{KM7:TR?= NIntemg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
MV-4-11 NH31ZXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfHTWM2OD16LkW0N|A1KM7:TR?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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RT-112 MlG3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlXpTWM2OD15LkG2OFU3KM7:TR?= NF:3PWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
CAL-85-1 M2LubGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE[0NlJKSzVyPU[uOlA6OzZizszN NVm0[IN7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
BB65-RCC M4qxSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVjiTodxUUN3ME22MlU{OTl3IN88US=> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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WM-115 MlLvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVy3c2h1UUN3ME21MlgyPTB7IN88US=> M4LVU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
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LB2518-MEL NVO2b4l4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn2wTWM2OD13LkeyOlI4KM7:TR?= NVy5OJlURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
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NCI-H2452 NGXybZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGDvN3NKSzVyPUWuOFU3OjhizszN MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
SW1710 NEnq[3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7MTINkUUN3ME21MlQzPDV4IN88US=> NG\qb4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
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RCC10RGB M4nFNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzSXmlyUUN3ME20MlI6PDd7IN88US=> NWj5[pA{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
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MHH-ES-1 NEfufXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\EU48zUUN3ME20MlA1PTR3IN88US=> Mmm3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
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MOLT-16 M1nyVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRTNwOEi1OVYh|ryP MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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HOP-62 NFjhfG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVWz[WdGUUN3ME2zMlM3ODV2IN88US=> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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SCC-9 NVPWTId3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2e0UGlEPTB;Mj65OVQ5PSEQvF2= NVvhOJhXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
COLO-829 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHX3PFhKSzVyPUKuO|Y2PCEQvF2= NWfNc2dvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
RPMI-7951 NEXnWpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLBfphKSzVyPUKuO|AyQTNizszN NH7LTG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
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SNU-423 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX:zXY43UUN3ME2yMlQ6Pzh5IN88US=> Mmj6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
NCI-H2122 NImySFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFu4PFNKSzVyPUKuNlc3QDRizszN MmjhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
RS4-11 M{TPfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXXzTmNEUUN3ME2xMlk2PDF3IN88US=> M4XtNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
HCC1395 NFjtfWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTFwN{iyO|Mh|ryP MoCzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MIA-PaCa-2 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHITWM2OD1zLke1PVc4KM7:TR?= NXf1XIp3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
KYSE-180 MkSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHK3R5dKSzVyPUGuO|I4QDhizszN NX7wXXZ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
BCPAP MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXjTWM2OD1zLk[2PFk5KM7:TR?= M{jSSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
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TYK-nu MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vObWlEPTB;MT6wO|kyOiEQvF2= MnnQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
D-247MG NWLsdlV2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWTmOndmUUN3ME2wMlk1QDV{IN88US=> NYniN4RrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
S-117 NVjsNWozT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHS2eJJKSzVyPUCuPVIyOzFizszN NHvUcYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
D-336MG NWHycWRbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3JTWM2OD1yLki0OlIh|ryP NV\MWmRiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
LOXIMVI MoH0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnXxTWM2OD1yLkW4PFMzKM7:TR?= MlLJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
769-P M17vVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4i0XmlEPTB;MD61PFQ6QSEQvF2= NHexcXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
GCT M3HlTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4m2OWlEPTB;MD6zNFY4OiEQvF2= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
SW982 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEn3V3JKSzVyPUCuNlE1OTZizszN MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
ES-CCE MUDGeY5kfGmxbjDBd5NigQ>? MXK1xsDPxE1? MlPFN{Bl NUfPXnZ4TE2VTx?= NEPkWI5UfHKxbnfsfUBqdmS3Y3XzJJRp\SCjY4Tpeol1gSCxZjDXcpQw[mW2YT3jZZRmdmmwIIDheIh4[Xl? M4\lVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{e5N|Y2Lz5{NEe3PVM3PTxxYU6=
ES-D3 MoHQSpVv[3Srb36gRZN{[Xl? MUO1xsDPxE1? M4n4fVMh\A>? MYnEUXNQ NIe4cGZUfHKxbnfsfUBqdmS3Y3XzJJRp\SCjY4Tpeol1gSCxZjDXcpQw[mW2YT3jZZRmdmmwIIDheIh4[Xl? M4HGV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{e5N|Y2Lz5{NEe3PVM3PTxxYU6=
ES-CCE NE[yd|NEgXSxdH;4bYMhSXO|YYm= M4TFNFExyqEQvF2= NVr3fo5{OyCm MYPEUXNQ NVjZZW5VUW6mdXPld{Bud2SncnH0[UBkgXSxdH;4bYNqfHl? NYLPcWR7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3O|k{PjVpPkK0O|c6OzZ3PD;hQi=>
ES-D3 NXnT[245S3m2b4TvfIlkKEG|c3H5 MWqxNOKh|ryP MonhN{Bl MkHYSG1UVw>? M13uVGlv\HWlZYOgcY9l\XKjdHWgZ5l1d3SxeHnjbZR6KHerdHigTWM2OCCxZjC0Mlkh|ryP NIiyVI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe3PVM3PSd-MkS3O|k{PjV:L3G+
Neural progenitor cells MU\GeY5kfGmxbjDBd5NigQ>? MYSzJO69VQ>? M1jGNVExKGR? NYXDWVB3TE2VTx?= NFniN2tKdmS3Y3XzJIdmdmW{YYTpc44hd2ZibnX1doFtKHC{b3flcol1d3JiY3XscJM> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ|OECzOEc,OjR4M{iwN|Q9N2F-
A549 MkniRZBweHSxc3nzJGF{e2G7 M3nxSFUhyrWP MluzNlQhcA>? MV3EUXNQ MmnVTY5kemWjc3XzJINmdGxiYYDvdJRwe2m| NEm5V2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2O|Y5QCd-MkSzOlc3QDh:L3G+
H157 M13CfmFxd3C2b4Ppd{BCe3OjeR?= NUnWWGFrPSEEtV2= M2q0XFI1KGh? NWPxV2wxTE2VTx?= NGTsNHpFd2W|IH7veEB{cWewaX\pZ4FvfGy7IHHm[oVkfCCjcH;weI9{cXN? MoPqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|Nke2PFgoRjJ2M{[3Olg5RC:jPh?=
H460 NG\QXpdCeG:ydH;zbZMhSXO|YYm= NIj2cXM2KML3TR?= NGjvPI4zPCCq MlnCSG1UVw>? Mne3RZR1\W63YYTld{Bk\WyuIHHwc5B1d3Orcx?= MlqxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|Nke2PFgoRjJ2M{[3Olg5RC:jPh?=
H157 NFjCUIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXW1JOK2VQ>? M2\6TlI1KGh? MnLBSG1UVw>? M2DDfHBzd22xdHXzJINmdGxicILvcIln\XKjdHnvci=> NYrjTXBORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlc3QDhpPkK0N|Y4Pjh6PD;hQi=>
H460 NWS3VHRUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHrBdW02KML3TR?= MmHENlQhcA>? MoW4SG1UVw>? MkizVJJwdW:2ZYOgZ4VtdCCycn;sbYZmemG2aX;u NXK0W3RKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlc3QDhpPkK0N|Y4Pjh6PD;hQi=>
embryonic stem cells (ESC) MlHiSpVv[3Srb36gRZN{[Xl? M{\Xc|MhyrWP M4\vcVEyKGR? MlLvSG1UVw>? MYfQdo9ud3SnczDz[YxnNXKnbnX3ZYwhd2ZibnJDs5ZmNXS7cHWgcY92e2ViZX3idplwdmmlIIP0[Y0h[2WubIOgLGVUSyl? M4TQfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OESzN|EzLz5{M{i0N|MyOjxxYU6=
DN3 MVvGeY5kfGmxbjDBd5NigQ>? MmfoNVAh|ryP MVq5OkBp NFXuUoFFVVOR MUjJcoNz\WG|ZYOgZ4VtdCCmZY\lcI9xdWWwdDDhcoQheHKxbHnm[ZJifGmxbh?= MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV{Nkm4PUc,OjN3Mk[5PFk9N2F-
platelets MYLGeY5kfGmxbjDBd5NigQ>? NWWzOJdSOTBizszN MkL5NVUhdWmw NFPoSJJFVVOR Mnj3TY5kemWjc3XzJJRpem:vYnnuMY1m\GmjdHXkJJBt[XSnbHX0JIFo\3KnZ3H0bY9vKGGwZDDBWHAhe2WlcnX0bY9v MoThQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{M{m4O|coRjJ|MkO5PFc4RC:jPh?=
hiPSC-NPs MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXOz5qCK|ryP MmTIO|IhcA>? NF3nV2pFVVOR NHPYVG9KdmO{ZXHz[ZMh[2WubDDwdo9tcW[ncnH0bY9vyqB? NX:0dY1XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFI3QDdpPkKyOlQzPjh5PD;hQi=>
INS-1E NYK0[WU4TnWwY4Tpc44hSXO|YYm= MWKyMlUh|ryP NWTG[lV4OjRiaB?= MXnEUXNQ NEXITmZKdmS3Y3XzJIEhemWmdXP0bY9vKG:oIF7QNUBwfmW{ZYjwdoV{e2mxbh?= MkLNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2Mke3NFQoRjJ{NEK3O|A1RC:jPh?=
PANC1 MmW5RZBweHSxc3nzJGF{e2G7 MXNCpFUh|ryP MUW3NkBp NVLhR2lSTE2VTx?= MorMWJJq\2encoOgZY4h[XCxcITveIlkKHKnc4DvcpNm M4e2c|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkCxNVg3Lz5{MkKwNVE5PjxxYU6=
Endothelial cell(EC) MUDGeY5kfGmxbjDBd5NigQ>? M3vQPFMh|ryP MlLLSG1UVw>? NYjhT|dRWHKxbX;0[ZMh\W6mb4To[Yxq[WxiY3XscEApTUNrIHTp[oZmemWwdHnheIlwdg>? MnzWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyNUKwNFYoRjJ{MEWyNFA3RC:jPh?=
HMC1.2 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1;0UFMxKM7:TR?= NH7aWVY4OiCq MVzEUXNQ NFLnfnlT\WS3Y3XzJINmdGxic4Xyeol3[Wx? NIHxXIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkCzPVMxOSd-MkKwN|k{ODF:L3G+
Sca-1+ HPC NUDCe|lDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2iyeFIh|ryP M1nhe|Yh\A>? NVS1fXJNTE2VTx?= NVrSR|lzW3mwZYLnbZN1cWOjbHz5JJBzd22xdHXzJHNk[S1zK9MgTHBEKHC{b3zp[oVz[XSrb36gZY5lKGOxbH;ufUBnd3KvYYTpc44hfG:pZYTo[ZIhf2m2aDDFS2Y> NETpcXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MU[yOFM1QCd-MkG2NlQ{PDh:L3G+
Oct4/Sox2/Klf4-infected MEFs MWPGeY5kfGmxbjDBd5NigQ>? MX2xNEDPxE1? M4\wVFI5KGR? MnX3SG1UVw>? MWrQdo9ud3SnczDNSWZ{KHKncILv[5JidW2rbnegWJJidnOmdXPl[EBjgSCRY4S0MEBUd3h{LDDhcoQhU2yoNB?= NGXxcmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUizPVA2PSd-MUm4N|kxPTV:L3G+
H4IIE M{HsOmZ2dmO2aX;uJGF{e2G7 M3jVN|ExKM7:TR?= MoDQNlQhcA>? NHrLOGtFVVOR MV3S[Yd2dGG2ZYOg{tIu[2G2ZX7pckBi[3Srdnn0fUBidmRiVFnSSUBkd262YXnubY5oKHC{b33veIVzKGGldHn2bZR6 MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTN3MEG5OUc,OTV|NUCxPVU9N2F-
insulin receptor–expressing CHO-IR MofhSpVv[3Srb36gRZN{[Xl? NH24b2k2ODBibl2= MX2zNEBucW5? NV7CR21WTE2VTx?= MYXBZ5RqfmG2ZYOgS4x6[2:pZX6gV5lvfGijc3WgLGdUMQ>? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjZyNkS5O{c,OTJ4ME[0PVc9N2F-
3T3-L1 MnriSpVv[3Srb36gRZN{[Xl? M4\iPVMh|ryP NI\WRpM1KGh? M1uzNWROW09? M1vYd2lvcGmkaYTzJJBz\WGmaYDvZ5l1\SCmaX\m[ZJmdnSrYYTpc44> NXTCb2RHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKwOVUzODBpPkGyNFU2OjByPD;hQi=>
CHO-IR MmWxSpVv[3Srb36gRZN{[Xl? M3Tt[VMxKG2rbh?= Mn[wSWM2OD1yLke3JO69VQ>? M1LXeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MEG2NVIyLz5{OUCxOlEzOTxxYU6=
HepG2 M2XpbGZ2dmO2aX;uJGF{e2G7 NYXn[nJXOyCq Mle2SWM2OD1zLkWg{txO M1;WcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[xNFI{Lz5{MkK2NVAzOzxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cleaved PARP / CD31 / VE-Cadherin / α-SMA / vimentin; 

PubMed: 30709379     


NCI-H460 cells were co-cultured with HUVECs in a 3D culture system, and then treated with 1 μM CHIR-99021, 10 μM or 20 μM Cisplatin, or a combination of both, for 24 h. Cells were harvested and immunoblotted with anti-cleaved PARP, anti-CD31, anti-VE-cadherin, anti-α-SMA, anti-vimentin, and anti-β-actin antibodies. 

CTNNB1; 

PubMed: 23993102     


Treatment with the GSK3β inhibitor CHIR-99021 led to increased levels of β-catenin in RKO and HT29 (non-mutant) cells.

β-catenin; 

PubMed: 24165128     


HeLa cells were treated with 5 μm CHIR-99021 for the indicated times. The samples were analyzed by Western blot.

30709379 23993102 24165128
Growth inhibition assay
Cell viability; 

PubMed: 22039301     


HMC-1.2 cells were cultured in the absence or presence of GSK3β inhibitor (CHIR 99021, 3-30 µM) and viability cell count was assessed by trypan blue dye exclusion after 24 h, 48 h or 72 h. All data are presented as the mean ± SEM of three independent experiments conducted in triplicate. p < 0.05*, p < 0.001** p < 0.0001*** vs vehicle treated control cells.

22039301
Immunofluorescence
SOX10/EdU; 

PubMed: 28174705     


Incorporation of EdU (white arrowheads) into SOX10+ ENS (enteric nervous system) cells with and without CHIR-99021.

Oct3/4; 

PubMed: 24779365     


Fluorescence micrographs showing the protein expression of Brachyury/Oct3/4 after a three day differentiation of ES-D3 cells with BIO (0.5 μM), SB-216763 (5 μM), CHIR-99021 (5 μM), or CHIR-98014 (1 μM). Nuclei were counterstained with DAPI (blue). The merge is shown in yellow. Original magnification 400x. Scale bar = 50 μm.

28174705 24779365
In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge[1].

Protocol

Cell Research:

[1]

- Collapse
  • Cell lines: Insulin receptor–expressing CHO-IR cells; Primary rat hepatocytes
  • Concentrations: 0.01-10 μM
  • Incubation Time: 30 min
  • Method:

    CHO-IR cells expressing human insulin receptor are grown to 80% confluence in Hamm’s F12 medium with 10% fetal bovine serum and without hypoxanthine. Trypsinized cells are seeded in 6-well plates at 1 × 106 cells/well in 2 ml of medium without fetal bovine serum. After 24 h, medium is replaced with 1 ml of serum-free medium containing GSK-3 inhibitor or control (final DMSO concentration <0.1%) for 30 min at 37°C. Cells are lysed and centrifuged 15 min at 4°C/14000g. The activity ratio of GS is calculated as the GS activity in the absence of glucose-6-phosphate divided by the activity in the presence of 5 mmol/l glucose-6-phosphate, using the filter paper assay of Thomas et al.


    (Only for Reference)
Animal Research:

[1]

- Collapse
  • Animal Models: Female db/db mice; Male ZDF rats
  • Dosages: 8-48 mg/kg
  • Administration: oral administration
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 10 mg/mL warmed (21.48 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
3.5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.34
Formula

C22H18Cl2N8

CAS No. 252917-06-9
Storage powder
in solvent
Synonyms N/A
Smiles CC1=CN=C(N1)C2=CN=C(N=C2C3=C(C=C(C=C3)Cl)Cl)NCCNC4=NC=C(C=C4)C#N

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID