Catalog No.S7198 Synonyms: GSK-3 Inhibitor IX, 6-bromoindirubin-3-oxime

BIO Chemical Structure

Molecular Weight(MW): 356.17

BIO is a specific inhibitor of GSK-3 with IC50 of 5 nM for GSK-3α/β in a cell-free assay, shows >16-fold selectivity over CDK5, also a pan-JAK inhibitor.

Size Price Stock Quantity  
USD 147 In stock
USD 570 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 4 Publications

2 Customer Reviews

  • Lysates of HCT116p53KO cells were harvested 24 hs after treatment with different GSK3 inhibitors and GSK3A/B activation/inactivation checked by western blot: a mix of pSer21-GSK3A and pSer9-GSK3B antibodies and antibody cross-reacting with both pTyr279-GSK3A and pTyr216-GSK3B were used to assess the specificity of the inhibitor for GSK3A. BIO: 6-bromoindirubin-3'-oxime, TWS: TWS119, SB2: SB216763, SB4: SB415286.

    PLoS One 2014 9(7), e100947. BIO purchased from Selleck.

    (e and f) Confocal immunofluorescence staining. U87 GSLCs and U251 GSLCs were seeded in suspension and treated with control, Tet, BIO or ICG-001 for 48 h. Blue fluorescence represents DAPI, red fluorescence represents β-catenin, and green fluorescence represents TUNEL. Scale bars, 40 μm

    Int J Oncol, 2017, 50(1):101-110. BIO purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description BIO is a specific inhibitor of GSK-3 with IC50 of 5 nM for GSK-3α/β in a cell-free assay, shows >16-fold selectivity over CDK5, also a pan-JAK inhibitor.
Features The first pharmacological agent shown to maintain self-renewal in human and mouse embryonic stem cells.
GSK-3 [1]
(Cell-free assay)
TYK2 [4]
(Cell-free assay)
CDK5/p35 [1]
(Cell-free assay)
CDK2/CyclinA [1]
(Cell-free assay)
CDK1/CyclinB [1]
(Cell-free assay)
5 nM 30 nM 0.08 μM 0.30 μM 0.32 μM
In vitro

BIO (6-bromoindirubin-3'-oxime) is a specific inhibitor of glycogen synthase kinase-3 (GSK-3), with IC50 of 5 nM for GSK-3α/β, shows >16-fold selectivity over CDK5. BIO interacts within the ATP binding pocket of these kinases, reduces β-catenin phosphorylation on a GSK-3-specific site in cellular models, closely mimicks Wnt signaling in Xenopus embryos. [1] In human and mouse embryonic stem cells, BIO maintains the undifferentiated phenotype and sustains expression of the pluripotent state-specific transcription factors Oct-3/4, Rex-1 and Nanog. BIO-mediated Wnt activation is functionally reversible, as withdrawal of the compound leads to normal multidifferentiation programs in both human and mouse embryonic stem cells. [2] BIO promotes proliferation in mammalian cardiomyocytes. [3]6BIO is also a pan-JAK inhibitor, with IC50 values of 0.03, 1.5, 8.0, 0.5 μM for TYK2, JAK1, JAK2 and JAK3. BIO selectively inhibits phosphorylation of STAT3 and induces apoptosis of human melanoma cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human SH-SY5Y cells NFPjbnFHfW6ldHnvckBie3OjeR?= NVnQdVg5UW6qaXLpeIlwdiCxZjDHV2s{NW2nZHnheIVlKGKndHGtZ4F{\WmwIIDoc5NxcG:{eXzheIlwdiCrbjDoeY1idiCVSD3TXVV[KGOnbHzzJIlvKHC{ZYPlcoNmKG:oIF3HNVMzKGK7IGfld5Rmem5iYnzveEBidmGueYPpd{whUUN3ME2wMlI6KM7:TR?= MYixPFgyPjFzMB?=
human K562 cells NYO5fnpiWHKxbHnm[ZJifGmxbjDhd5NigQ>? NXr2e2xvPzJiaB?= NH7udGRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFu1OlIh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zLkOg{txO MXKxPVc5OzF2OR?=
human IMR90 cells NFL2ZYJRem:uaX\ldoF1cW:wIHHzd4F6 MV23NkBp NH3QcXhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFnNVlkxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MT65JO69VQ>? NGP4S3kyQTd6M{G0PS=>
human HCT116 cells M{jhcnBzd2yrZnXyZZRqd25iYYPzZZk> NX73T2xHPzJiaB?= MmThRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKQ2SxNVYh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD13LkKg{txO NFLuS3AyQTd6M{G0PS=>
human HL60 cells NUTsUJh7WHKxbHnm[ZJifGmxbjDhd5NigQ>? M{\lNVUh\GG7cx?= NVf2VW9YSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IEWg[IF6eyCkeTDNWHQh[XO|YYm= M1Lv[VE6Pzh|MUS5
human HuH7 cells MoHxVJJwdGmoZYLheIlwdiCjc4PhfS=> MVy3NkBp Mof1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKdVi3JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[Xl? NHTPc5MyQTd6M{G0PS=>
human SH-SY5Y cells MYDDfZRwfG:6aXRCpIF{e2G7 M1\B[FQ5KGh? NXXHdWFSS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0hvU2m1XUBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTTJJJm\HWldHnvckBie3OjeTygTWM2OD17IN88US=> MlfiNVg5OTZzMUC=
human NB39 cells M{DQ[2N6fG:2b4jpZ:Kh[XO|YYm= NUnmTW13PDhiaB?= NGXkT2hEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBPSjN7IHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZk> MofpNlE5ODJ7NEe=
human SK-N-SH cells MlHPR5l1d3SxeHnjxsBie3OjeR?= MnvtOFghcA>? MXvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT{1PNVOKIHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZk> MlHBNlE5ODJ7NEe=
mouse HT22 cells M125TWZ2dmO2aX;uJIF{e2G7 NGjIco8yOCEQvF2= MY[yOEBp MnrDTY5pcWKrdHnvckBw\iCJU1uzMY1m\GmjdHXkJIJmfGFiY3Hz[YlvKHCqb4PwbI9zgWyjdHnvckBqdiCvb4Xz[UBJXDJ{IHPlcIx{KGG2IEGwJJVOKGGodHXyJFI1KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXNiaX6gdJJme2WwY3Wgc4YhVUdzM{K= MXmyNlk6QDR2Mx?=
HEK293 cells NEO2cVlHfW6ldHnvckBie3OjeR?= M325ZlAvPS1zIN88US=> MoOxTY5pcWKrdHnvckBw\iCqdX3hckBIW0t|IHHjeIl3cXS7IHnuJGhGUzJ7MzDj[YxteyClb370ZYlvcW6pIITo[UBYdnRxYnX0ZU1k[XSnbnnuJIFkfGm4YYTl[EBz\XCxcoTldkBxW3WyZYLUU3BHVEGVSDCoV3RHOjl|IHPlcIx{MSCjdDCwMlUhfG9iMTD1UUBjgSCZboSgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> MoHGNlQ3QTd{NES=

... Click to View More Cell Line Experimental Data

In vivo BIO suppresses melanoma tumor growth in a mouse xenograft model. [4]


Kinase Assay:[1]
+ Expand

Kinase assay:

Kinase activities are assayed in Buffer A or C at 30°C, at a final ATP concentration of 15 μM. Blank values are subtracted and activities calculated as pmoles of phosphate incorporated during a 10 min incubation. Controls are performed with appropriate dilutions of dimethylsulfoxide. In a few cases phosphorylation of the substrate is assessed by autoradiography after SDS-PAGE. GSK-3α/β is purified from porcine brain by affinity chromatography on immobilized axin. It is assayed, following a 1/100 dilution in 1 mg BSA/ml 10 mM DTT, with 5 μl 40 μM GS-1 peptide, a specific GSK-3 substrate, (YRRAAVPPSPSLSRHSSPHQSpEDEEE), in buffer A, in the presence of 15 μM [γ-32P] ATP (3,000 Ci/mmol; 1 mCi/ml) in a final volume of 30 μl. After 30 min incubation at 30°C, 25 μl aliquots of supernatant are spotted onto 2.5 × 3 cm pieces of Whatman P81 phosphocellulose paper, and 20 seconds later, the filters are washed five times (for at least 5 min each time) in a solution of 10 ml phosphoric acid/liter of water. The wet filters are counted in the presence of 1 ml ACS scintillation fluid.
Cell Research:[1]
+ Expand
  • Cell lines: COS1, Hepa or SH-SY5Y cells
  • Concentrations: ~10 μM
  • Incubation Time: 12 or 24 h
  • Method: COS1, Hepa (wild-type, CEM/LM AhR deficient and ELB1 ARNT deficient), or SH-SY5Y cells are grown in 6 cm culture dishes in Dulbecco's Modified Medium (DMEM) containing 10% fetal bovine serum. For treatment, IO (5 μM), BIO (5 or 10 μM), MeBIO (5 or 50 μM), LiCl (20 or 40 mM), or mock solution (DMSO, 0.5% final concentration) is added to medium when cell density reaches ∼70% confluence. After 12 (SH-SY5Y) or 24 hours, the cells, while still in plate, are lysed with lysis buffer (1% SDS, 1 mM sodium orthovanadate, 10 mM Tris [pH 7.4]). The lysate is passed several times through a 26G needle, centrifuged at 10,000 × g for 5 min, and adjusted to equal protein concentration. About 8 μg of each sample is loaded for immunoblotting. Enhanced chemiluminescence is used for detection. The following primary antibodies are used: mouse anti-β-catenin CT (Upstate Biotechnolgies, Clone 7D8, recognizes total β-catenin), mouse anti-phospho-β-catenin (Upstate Biotechnologies, Clone 8E7, recognizes dephosphorylated β-catenin), mouse anti-GSK-3 β, mouse anti-GSK-3 phosphoTyr216, rabbit anti-AhR (Aryl hydrocarbon receptor), and rabbit anti-actin.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: mouse
  • Formulation: freshly prepared in 30% Solutol (Basf) at a concentration of 10 mg/mL.
  • Dosages: 50 mg/kg
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 71 mg/mL (199.34 mM)
Ethanol 6 mg/mL (16.84 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 356.17


CAS No. 667463-62-9
Storage powder
in solvent
Synonyms GSK-3 Inhibitor IX, 6-bromoindirubin-3-oxime

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03789721 Not yet recruiting ALD (Adrenoleukodystrophy) Masonic Cancer Center University of Minnesota February 2020 --
NCT03789721 Not yet recruiting ALD (Adrenoleukodystrophy) Masonic Cancer Center University of Minnesota February 2020 --
NCT03875729 Not yet recruiting Type 1 Diabetes Mellitus Provention Bio Inc. June 2019 Phase 3
NCT03875729 Not yet recruiting Type 1 Diabetes Mellitus Provention Bio Inc. June 2019 Phase 3
NCT03761394 Not yet recruiting Stroke|Atrial Fibrillation University of Massachusetts Worcester|University of Connecticut|Northeastern University|Brigham and Women''s Hospital|National Heart Lung and Blood Institute (NHLBI) May 2019 Not Applicable
NCT03865017 Not yet recruiting Alzheimer Disease GMP BIO Co. LTD.|BHT Lifescience Austrailia Pty Ltd. May 21 2019 Phase 1|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

GSK-3 Signaling Pathway Map

Related GSK-3 Products0

Tags: buy BIO | BIO supplier | purchase BIO | BIO cost | BIO manufacturer | order BIO | BIO distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID