Y-27632 2HCl

Catalog No.S1049

For research use only.

Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.

Y-27632 2HCl Chemical Structure

CAS No. 129830-38-2

Selleck's Y-27632 2HCl has been cited by 776 publications

Purity & Quality Control

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Biological Activity

Description Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.
Targets
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
In vitro

Y-27632 2HCl inhibits ROCK-II while displaying little activity against PKC, cAMP-dependent protein kinase and myosin light-chain kinase (MLCK) with Ki of 26 μM, 25 μM and > 250 μM, respectively, as well as PKA activated by another Rho-family GTPase member, Cdc42. Y-27632 2HCl inhibits smooth-muscle contraction induces by various agonists including phenylephrine, histamine, acetylcholine, serotonin, endothelin, and thromboxane with IC50 of 0.3-1 μM, by selectively inhibiting Ca2+ sensitization. Y-27632 2HCl suppresses Rho-induced, p160ROCK-mediated formation of stress fibres in cultured cells. [1] Y-27632 2HCl treatment blocks both Rho-mediated activation of actomyosin and LPA-stimulated invasive activity of MM1 cells in a concentration-dependent manner. [2] Y-27632 2HCl treatment is not only sufficient to initiate formation of exuberant axonal processes but also facilitates axonal maturation during the very early stages of axonogenesis, while largely sparing axon elongation. [3] In human embryonic stem (hES) cells, Y-27632 2HCl treatment at 10 μM markedly diminishes dissociation-induced apoptosis even in serum-free suspension (SFEB) culture, increases cloning efficiency (from ~1% to ~27%), facilitates subcloning after gene transfer, and enables SFEB-cultured hES cells to survive and differentiate into Bf1+ cortical and basal telencephalic progenitors. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Salivary gland stem cells NH7BSmZHfW6ldHnvckBCe3OjeR?= NFvLW5EyOCEEtV2= M3X0S|ch\A>? NULjS5FUWmWmdXPld{BUT1OFIIPlcoV{[2WwY3W= NXzLSnZWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4NFQ2PjBpPkK1PFA1PTZyPD;hQi=>
HT22 MVrDfZRwfG:6aXOgRZN{[Xl? NYPPRlhtOTBiwsXN Ml\hNVMhcA>? NVPWUYJ7WHKxdHXjeJMh[WejaX7zeEBodHW2YX3heIUucW6mdXPl[EBv\XW{b37hcEBl\WG2aB?= NGHFNoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkixNFg{PSd-MkK4NVA5OzV:L3G+
Swiss3T3 MoXOR49td267LX\vdo1qdmdiQYPzZZk> NHPJUmcyOCEEtV2= NXWxXI1xOTNiZB?= M1H2PGlv[3KnYYPld{Bxem:|dHH0[UBk\WyuIHPvcI9vgS2ob4LtbY5oKGGldHn2bZR6 NX7aWpRzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0OlQ6ODJpPkKxOFY1QTB{PD;hQi=>
TSGH 8301 MoPTUYloemG2aX;uJGF{e2G7 MVOyNEDDvU1? Mmf0NUBp MkfxTY5kemWjc3XzJINmdGxibXnndoF1cW:w NVrmb2ZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4PVY1PzVpPkG5PFk3PDd3PD;hQi=>
Cynomolgus monkey embryonic stem cells NEHSfIdEgXSxdH;4bYMhSXO|YYm= MWOyNEDDvU1? MYqyOEBp Mn\6VJJwdW:2ZYOgZ5lGWyClZXzsJJN2en[rdnHs M4XGUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OUSwPFU2Lz5zOEm0NFg2PTxxYU6=
PC 12 MnS1SpVv[3Srb36gRZN{[Xl? NXjtOllHOTEEoN88US=> MUSyOEBp MoPaRZR1\W63YYTld{Bk[XSnY3jvcIFucW6nIHLpc5N6dnSqZYPpdy=> M4fhUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4MkG5OFI1Lz5zNkKxPVQzPDxxYU6=
C2C12 MYfGeY5kfGmxbjDBd5NigQ>? MkO3NVDDqM7:TR?= NVTUNlQxPiCq MXzQdoV3\W62czD0bIUhe2W{aX7lJJBpd3OyaH;yfYxifGmxbjDv[kBKWlNvMTDpcoR2[2WmIHL5JIlve3WuaX6gZY5lN2:{IGTOSk3PuQ>? M1XpOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4Mk[3NVI1Lz5zNkK2O|EzPDxxYU6=
Pancreatic acinar cells MlzFSpVv[3Srb36gRZN{[Xl? M{HE[FExyqEQvF2= NFXHcI44OCCvaX6= MY\Qc5RmdnSrYYTld{BES0tvc4TpcZVt[XSnZDDwZY5kemWjdHnjJIVvgnmvZTDz[YNz\XSrb36= NEP6bow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMke0OVA5OCd-MUK3OFUxQDB:L3G+
Rat hepatic stellate cells M2T1fGZ2dmO2aX;uJGF{e2G7 Mm[xN|Ah|ryP M4\UZlQ5KGh? NHrYXmNFcW2rbnnzbIV{KHSqZTDwbI9{eGixconsZZRqd25ib3[gSZJsOixiYX7kJIRm[3KnYYPld{Bv\XdiRF7BJJN6dnSqZYPpdy=> NI\xbIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEi0OVY3Oyd-MUC4OFU3PjN:L3G+
LNCaP NILRV5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWWyOUDPxE1? MXKxO{Bp M3nOSGRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NI[wVXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEeyNFQ4OSd-MUC3NlA1PzF:L3G+
PC3 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXSyOUDPxE1? NGLFcFAyPyCq M{PFXWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NFLZdJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEeyNFQ4OSd-MUC3NlA1PzF:L3G+
PC3 NWf4dJJmVWmpcnH0bY9vKEG|c3H5 M3rzSVI2KM7:TR?= NEK1bZkyKGh? NVfH[I9nUW6qaXLpeJMhfGinIFLNSmIuS01iYX7kJJRp\SCHR1[td5RqdXWuYYTl[EBucWe{YYTpc44> M2PUflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyN{KwOFcyLz5zMEeyNFQ4OTxxYU6=
PC3 NFHmW3BHfW6ldHnvckBCe3OjeR?= NXLqVFFQOjVizszN NVTzTmw4OSCq NW\NdWxKUW6mdXPld{Bud3KyaH;sc4dq[2GuIHPoZY5o\XN? M{PDOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyN{KwOFcyLz5zMEeyNFQ4OTxxYU6=
Rat Vascular Smooth Muscle Cells NYfmd5FSTnWwY4Tpc44hSXO|YYm= NXv3VWVCOTBizszN NGHqPHEzKGh? M4\XN2lvcGmkaYTzJIFv\2mxdHXud4lvKEmLLXnu[JVk\WRiaInw[ZJ1em:yaIm= NVrHeZFXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC2OFI{OTdpPkGwOlQzOzF5PD;hQi=>
Stellate Cell NXTVNWxVTnWwY4Tpc44hSXO|YYm= NUnHWmFnOjVizszN MnXxNVUhdWmw MnrpTY5pcWKrdIOg[o9zdWG2aX;uJI9nKEZvYXP0bY4he3S{ZYPzJIZq[mW{czDhcoQheGixc4Doc5J6dGG2aX;uJI9nKG27b4PpckBtcWeqdDDjbIFqdg>? NX3pfYF3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC2NFA1QTZpPkGwOlAxPDl4PD;hQi=>
Neonatal rat ventricular myocytes M3Lp[GZ2dmO2aX;uJGF{e2G7 M{L0e|ExKM7:TR?= NHT1cGU1QCCq Mn\BTY5pcWKrdIOgSXQuOS2rbnT1Z4VlKGmwY4LlZZNmeyCrbjDwdo91\WmwIIP5cpRp\XOrczygZ4VtdCC|aYrlJIFv\CCveX;mbYJzcWyuYYKgc5Jo[W6renH0bY9v M2Dh[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyM{i2OlE{Lz5zMEO4OlYyOzxxYU6=
LS174T NF7qcWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIO5ZWEyOCEQvF2= M1P0VVE5KGR? M2[wc21w\GW{YYTlcJkhcW6qaXLpeJMh[2WubDDndo94fGh? MlXrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
HCT116 M1z1bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXuxNEDPxE1? Mn;lNVgh\A>? M1\CPHN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp NIfsSWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
HCT15 NX;YOHkxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXuxNEDPxE1? NEnwfHIyQCCm NEXTW4FFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NYXlSZV[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
SW620 M4jxR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\vSlExKM7:TR?= MnnVNVgh\A>? MkHNSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> Mk\qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
Src-2 Ml3ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;HZ|cyOCEQvF2= NH3GfYwyQCCm Mo\USI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> M4rIdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
Src-1 NGjaeZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3uXJcyOCEQvF2= M3nMXVE5KGR? NXXQfWhpTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MmnGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
NIH3T3 MonhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX2xNEDPxE1? NXnI[4VSOThiZB?= MorPSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
Src-4 Mn;IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWqxNEDPxE1? NUjlNYprOThiZB?= M3zLSmRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NIC0d3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
Src-1 M{Cz[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnHlNVAh|ryP MWexPEBl M2fvTWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NUfHSlFuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Ras-4 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{XacVExKM7:TR?= NHe1R5YyQCCm Mk[xV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> NUDWdpNQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Ras-2 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLwNVAh|ryP NWn6elRvOThiZB?= MWXTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> NHq1clA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
mNET1-e Mm\4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3WzUlExKM7:TR?= NV\uWpFEOThiZB?= MlXWV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> M3zVc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
mNET1-d MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{TROlExKM7:TR?= NWHy[4FCOThiZB?= MUPTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> NYLVfJlFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Dbl-e M1fHRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4exe|ExKM7:TR?= MljDNVgh\A>? MljUUY9l\XKjdHXsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
Dbl-d NG\SfFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXBNVAh|ryP NF3JfZMyQCCm MlXFV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> NYnvVGI5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
NIH3T3 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DmblExKM7:TR?= NVHTVnF4OThiZB?= MVHEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To NVTu[mlqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Mesothelial cells from rat mesentery NEDjTYhKdn[jc3n2[UBCe3OjeR?= MUKzNEDPxE1? MWmyNEBp M2PF[mJtd2OtczDpcpZie2m4ZTDhZ5Rqfmm2eR?= M{fHTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl7M{C4O|IoRjl7M{C4O|I9N2F-
CCL39 NHHWZ5FHfW6ldHnvckBCe3OjeR?= MoPtN|Ah|ryP MnKyN|AhdWmw NGTERVlEd22ybHX0[Yx6KGGkb3zpd4hmeyCjY4TpeoF1cW:wIH;mJG5iNUhiZYjjbIFv\2W{IF7ISVEh[nliaX70[YdzcW6| NILGVWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97NkmzN|gzLz57NkmzN|gzRC:jPh?=
HeLa M1PPUWZ2dmO2aX;uJGF{e2G7 NE\TSZoyOCEQvF2= MYizNEBucW5? MmTQTY5pcWKrdIOgeIhmKG[xcn3heIlwdiCxZjDzeJJme3NiZnni[ZJ{KGGwZDD0bIUh[XO|ZX3icJkhd2ZidnnuZ5VtcW5vY3;ueIFqdmmwZzDmc4NidCCjZHjld4lwdnN? M3rNTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl4NkiwO|IoRjl4NkiwO|I9N2F-
N1E-115 MVLGeY5kfGmxbjDBd5NigQ>? NF7oZnAyOCEQvF2= MnizNkBp M3XP[GROW09? NET4[2lKdmirYnn0d{B1cGViYYPz[Y1jdHlib3[gcYlkem:2dXL1cIV{KGGwZDDpcpRmem2nZHnheIUh\mmuYX3lcpR{KHSxIH\vdo0h\Xi2ZX7k[YQheHKxY3Xzd4V{ Ml33QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTZ2N{[1OEc,QTZ2N{[1OFww[T5?
Swiss 3T3 cells M3XXTGZ2dmO2aX;uJGF{e2G7 NUG2V2k{OTBizszN MWGyJIg> MlPXSG1UVw>? MoXGTY5pcWKrdIOgeIhmKGG|c3XtZox6KG:oIH3pZ5JwfHWkdXzld{BidmRiaX70[ZJu\WSrYYTlJIZqdGGvZX70d{B1dyCob4LtJIV5fGWwZHXkJJBzd2Onc4Pldy=> NY\xS5N3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOU[0O|Y2PCd-OU[0O|Y2PDxxYU6=
Assay
Methods Test Index PMID
Western Blot p-MLC2(Ser19) ; E-cadherin ; ROCK2 ; KRT7 ; CDK2 ; p-Cofilin(Ser3) ; p-LIMK2(Thr505) ; p-LIMK1(Thr508) 27399334 26555939 24704720
Transwell migration assay cell migration inhibition 27694793
Immunofluorescence Phalloidin ; beta-catenin ; E-cadherin ; Neurotoxicity Assay 27399334 24523903 21362567
Growth inhibition assay cell proliferation 24523903
ELISA caspase-9 30320378
In vivo Oral administration of Y-27632 2HCl at 30 mg/kg significantly decreases the blood pressure in a dose-dependent manner in spontaneous hypertensive rats, renal hypertensive rats, as well as deoxycorticosterone acetate (DOCA)-salt hypertensive rats. [1] When Y-27632 2HCl is continuously administered at a rate of 0.55 μL per hour by implanted pumps for 11 days tumor cell invasion (MM1 cells expressing Val14-RhoA in rats) is significantly delayed. [2] By inhibiting ROCK, Y-27632 2HCl treatment attenuates hypoxia-induced angiogenesis and vascular remodeling in the pulmonary circulation. [5] Pretreatment with Y-27632 has a protective effect against tumor formation in albino mice with Ehrlich ascites carcinoma. [7]

Protocol (from reference)

Animal Research:[1] [7]
  • Animal Models: Male Wistar rats with spontaneous or induced hypertension; Swiss albino mice with Ehrlich ascites carcinoma
  • Dosages: 30 mg/kg/day (Rat); 0-10 mg/kg (mice)
  • Administration: Orally (Rat); i.p. (Mice)
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 64 mg/mL warmed
(199.83 mM)
Water 14 mg/mL
(43.71 mM)
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
saline
For best results, use promptly after mixing.

10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 320.26
Formula

C14H21N3O.2HCl

CAS No. 129830-38-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C1CCC(CC1)C(=O)NC2=CC=NC=C2)N.Cl.Cl

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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Frequently Asked Questions

Question 1:
Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

Answer:
The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

Question 2:
Could this product be used in cell culture? Do you have any reference for this application?

Answer:
Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

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