Y-27632 2HCl

Catalog No.S1049

For research use only.

Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.

Y-27632 2HCl Chemical Structure

CAS No. 129830-38-2

Selleck's Y-27632 2HCl has been cited by 865 publications

Purity & Quality Control

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Biological Activity

Description Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.
Targets
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
In vitro

Y-27632 2HCl inhibits ROCK-II while displaying little activity against PKC, cAMP-dependent protein kinase and myosin light-chain kinase (MLCK) with Ki of 26 μM, 25 μM and > 250 μM, respectively, as well as PKA activated by another Rho-family GTPase member, Cdc42. Y-27632 2HCl inhibits smooth-muscle contraction induces by various agonists including phenylephrine, histamine, acetylcholine, serotonin, endothelin, and thromboxane with IC50 of 0.3-1 μM, by selectively inhibiting Ca2+ sensitization. Y-27632 2HCl suppresses Rho-induced, p160ROCK-mediated formation of stress fibres in cultured cells. [1] Y-27632 2HCl treatment blocks both Rho-mediated activation of actomyosin and LPA-stimulated invasive activity of MM1 cells in a concentration-dependent manner. [2] Y-27632 2HCl treatment is not only sufficient to initiate formation of exuberant axonal processes but also facilitates axonal maturation during the very early stages of axonogenesis, while largely sparing axon elongation. [3] In human embryonic stem (hES) cells, Y-27632 2HCl treatment at 10 μM markedly diminishes dissociation-induced apoptosis even in serum-free suspension (SFEB) culture, increases cloning efficiency (from ~1% to ~27%), facilitates subcloning after gene transfer, and enables SFEB-cultured hES cells to survive and differentiate into Bf1+ cortical and basal telencephalic progenitors. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Salivary gland stem cells NXnBbVg1TnWwY4Tpc44hSXO|YYm= MliyNVAhyrWP NWLlN5FiPyCm NFe4SYpT\WS3Y3XzJHNIW0Nic3Xu[ZNk\W6lZR?= MmjZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MES1OlAoRjJ3OEC0OVYxRC:jPh?=
HT22 MXXDfZRwfG:6aXOgRZN{[Xl? NV3OR5c4OTBiwsXN Mkm5NVMhcA>? M171fnBzd3SnY4TzJIFo[Wmwc4Sg[4x2fGGvYYTlMYlv\HWlZXSgcoV2em:wYXyg[IVifGh? Mmj0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6MUC4N|UoRjJ{OEGwPFM2RC:jPh?=
Swiss3T3 MYrDc4xwdnlvZn;ycYlv\yCDc4PhfS=> NInNbncyOCEEtV2= NELRVJUyOyCm MUjJcoNz\WG|ZYOgdJJwe3SjdHWgZ4VtdCClb3zvcpku\m:{bXnu[{Bi[3Srdnn0fS=> M1nYeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNE[0PVAzLz5{MUS2OFkxOjxxYU6=
TSGH 8301 NYL4PJdrVWmpcnH0bY9vKEG|c3H5 MYSyNEDDvU1? NXi0TYp1OSCq MlfZTY5kemWjc3XzJINmdGxibXnndoF1cW:w MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh7NkS3OUc,OTl6OU[0O|U9N2F-
Cynomolgus monkey embryonic stem cells Mn;sR5l1d3SxeHnjJGF{e2G7 M3PHZVIxKML3TR?= NIK5[GYzPCCq MWLQdo9ud3SnczDjfWVUKGOnbHygd5Vzfmm4YXy= M4DvNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OUSwPFU2Lz5zOEm0NFg2PTxxYU6=
PC 12 NV\WUoIyTnWwY4Tpc44hSXO|YYm= MmLxNVDDqM7:TR?= MVyyOEBp M4L1dGF1fGWwdXH0[ZMh[2G2ZXPoc4xidWmwZTDibY9{gW62aHXzbZM> NHvF[4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkKxPVQzPCd-MU[yNVk1OjR:L3G+
C2C12 NXXLPFlZTnWwY4Tpc44hSXO|YYm= MUexNOKh|ryP NYnXOo5yPiCq MV\QdoV3\W62czD0bIUhe2W{aX7lJJBpd3OyaH;yfYxifGmxbjDv[kBKWlNvMTDpcoR2[2WmIHL5JIlve3WuaX6gZY5lN2:{IGTOSk3PuQ>? NWPFWGx1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[yOlcyOjRpPkG2NlY4OTJ2PD;hQi=>
Pancreatic acinar cells MVHGeY5kfGmxbjDBd5NigQ>? MWqxNOKh|ryP MX23NEBucW5? NUS4VplXWG:2ZX70bYF1\XNiQ1PLMZN1cW23bHH0[YQheGGwY4LlZZRq[yCnbor5cYUhe2WlcnX0bY9v NGDwSlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMke0OVA5OCd-MUK3OFUxQDB:L3G+
Rat hepatic stellate cells MnOySpVv[3Srb36gRZN{[Xl? M4fQTlMxKM7:TR?= NXnDfVQyPDhiaB?= NEjxfoFFcW2rbnnzbIV{KHSqZTDwbI9{eGixconsZZRqd25ib3[gSZJsOixiYX7kJIRm[3KnYYPld{Bv\XdiRF7BJJN6dnSqZYPpdy=> MkX2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB6NEW2OlMoRjFyOES1OlY{RC:jPh?=
LNCaP NHy2Vm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXSyOUDPxE1? M1vhe|E4KGh? NH;yVIRFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NX\IWFNsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC3NlA1PzFpPkGwO|IxPDdzPD;hQi=>
PC3 NGTOfVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnq[okzPSEQvF2= NIi5WnAyPyCq NHew[|BFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp M125S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyN{KwOFcyLz5zMEeyNFQ4OTxxYU6=
PC3 NGPKc4tOcWe{YYTpc44hSXO|YYm= NXfRdGlYOjVizszN NIXHZVYyKGh? M2WzPGlvcGmkaYTzJJRp\SCETV\CMWNOKGGwZDD0bIUhTUeILYP0bY12dGG2ZXSgcYloemG2aX;u NIrRe2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEeyNFQ4OSd-MUC3NlA1PzF:L3G+
PC3 NEDoWYNHfW6ldHnvckBCe3OjeR?= M{Dkd|I2KM7:TR?= M1jzWFEhcA>? NVXYeJBtUW6mdXPld{Bud3KyaH;sc4dq[2GuIHPoZY5o\XN? NWjIWIExRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC3NlA1PzFpPkGwO|IxPDdzPD;hQi=>
Rat Vascular Smooth Muscle Cells MULGeY5kfGmxbjDBd5NigQ>? M1:5PFExKM7:TR?= NYXUb3AyOiCq NWXVXHdOUW6qaXLpeJMh[W6paX;0[Y5{cW5iSVmtbY5lfWOnZDDofZBmenS{b4DofS=> MmP1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB4NEKzNVcoRjFyNkSyN|E4RC:jPh?=
Stellate Cell MWPGeY5kfGmxbjDBd5NigQ>? Mn\aNlUh|ryP M2Kz[VE2KG2rbh?= MX7Jcohq[mm2czDmc5Ju[XSrb36gc4YhTi2jY4TpckB{fHKnc4Og[olj\XK|IHHu[EBxcG:|cHjvdplt[XSrb36gc4YhdXmxc3nuJIxq\2i2IHPoZYlv MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODZyMES5Okc,OTB4MEC0PVY9N2F-
Neonatal rat ventricular myocytes MnfmSpVv[3Srb36gRZN{[Xl? NXLOV|JYOTBizszN NVj6OHhrPDhiaB?= NHLofHZKdmirYnn0d{BGXC1zLXnu[JVk\WRiaX7jdoVie2W|IHnuJJBzd3SnaX6gd5lvfGinc3nzMEBk\WyuIIPpfoUh[W6mIH35c4Zq[nKrbHzhdkBwemejbnn6ZZRqd25? M3LHeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyM{i2OlE{Lz5zMEO4OlYyOzxxYU6=
LS174T NGrvW2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\4UlExKM7:TR?= M1XPTVE5KGR? MVnNc4RmemG2ZXz5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp M4TaVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
HCT116 M4nKXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHPQWZMyOCEQvF2= M4HQN|E5KGR? NXzqVFJXW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? NEXzTYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
HCT15 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXKxNEDPxE1? Mo\0NVgh\A>? M2rLcGRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NFPFN3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
SW620 M13HR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3TOTFExKM7:TR?= NWTzWnh[OThiZB?= NU[4NlA5TG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? NXPsc3YyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Src-2 NHXvdXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVyxNEDPxE1? MXyxPEBl NH3ERZpFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NWTHW5dqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Src-1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3JfHZLOTBizszN NXyzbnN7OThiZB?= MV7Ec4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To NIi1V4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
NIH3T3 NHP2WYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXi2cFg5OTBizszN M1H4blE5KGR? MVzEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To M1;BV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
Src-4 MmniS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYOxNEDPxE1? MmD2NVgh\A>? M3W1dWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
Src-1 MnvIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUexNEDPxE1? M3rJeVE5KGR? MnqySI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> NVrTTWVbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Ras-4 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXvNVAh|ryP NXjjN2RGOThiZB?= NImx[HJUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? MmXqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
Ras-2 NGOwbFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{CxbVExKM7:TR?= M3mxTlE5KGR? M1vZcHN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp NUS0RmdFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
mNET1-e MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYnR[WRbOTBizszN NFnGUY0yQCCm Mnm2V5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> NF3s[oU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
mNET1-d M1jFTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYXITYZJOTBizszN M1;jRlE5KGR? M3jROHN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp M3LkRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
Dbl-e NFG2[2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVqxNEDPxE1? MVexPEBl NVvvOpo2VW:mZYLheIVtgSCrbnjpZol1eyClZXzsJIdzd3e2aB?= NV;i[YFZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Dbl-d M3vCb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3npcVExKM7:TR?= MUKxPEBl M3XTSXN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp NEDaW|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
NIH3T3 M1TMUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYmxNEDPxE1? NGr1RpMyQCCm Mne4SI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> NEDsNJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
Mesothelial cells from rat mesentery M{\LU2lvfmG|aY\lJGF{e2G7 Mn\4N|Ah|ryP MX[yNEBp MWjCcI9kc3NiaX72ZZNqfmViYXP0bZZqfHl? NV3KfVR2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUmzNFg4Oid-OUmzNFg4OjxxYU6=
CCL39 MVPGeY5kfGmxbjDBd5NigQ>? NG\hUoM{OCEQvF2= MoriN|AhdWmw M3G5cmNwdXCuZYTlcJkh[WKxbHnzbIV{KGGldHn2ZZRqd25ib3[gUoEuUCCneHPoZY5o\XJiTljFNUBjgSCrboTl[5JqdnN? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86Pjl|M{iyK|46Pjl|M{iyQE9iRg>?
HeLa NIm2VmpHfW6ldHnvckBCe3OjeR?= NITubowyOCEQvF2= M2exbFMxKG2rbh?= M{\MSmlvcGmkaYTzJJRp\SCob4LtZZRqd25ib3[gd5Rz\XO|IH\pZoVzeyCjbnSgeIhmKGG|c3XtZox6KG:oII\pcoN2dGmwLXPvcpRicW6rbneg[o9k[WxiYXTo[ZNqd26| MljLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTZ4OEC3Nkc,QTZ4OEC3Nlww[T5?
N1E-115 NGq3N5VHfW6ldHnvckBCe3OjeR?= NGPPWXgyOCEQvF2= NV74VFJCOiCq NV30ZoNqTE2VTx?= MYTJcohq[mm2czD0bIUh[XO|ZX3icJkhd2ZibXnjdo91fWK3bHXzJIFv\CCrboTldo1m\GmjdHWg[olt[W2nboTzJJRwKG[xcn2g[Zh1\W6mZXSgdJJw[2W|c3Xz NEHKNFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97NkS3OlU1Lz57NkS3OlU1RC:jPh?=
Swiss 3T3 cells NV31VINsTnWwY4Tpc44hSXO|YYm= M2HXRlExKM7:TR?= Mk\zNkBp MmLGSG1UVw>? NELySGpKdmirYnn0d{B1cGViYYPz[Y1jdHlib3[gcYlkem:2dXL1cIV{KGGwZDDpcpRmem2nZHnheIUh\mmuYX3lcpR{KHSxIH\vdo0h\Xi2ZX7k[YQheHKxY3Xzd4V{ MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86PjR5NkW0K|46PjR5NkW0QE9iRg>?
Assay
Methods Test Index PMID
Western Blot p-LIMK1(Thr508) ; p-LIMK2(Thr505) ; p-Cofilin(Ser3) ; CDK2 ; KRT7 ; ROCK2 ; E-cadherin ; p-MLC2(Ser19) 24704720 26555939 27399334
Transwell migration assay cell migration inhibition 27694793
Immunofluorescence Neurotoxicity Assay ; E-cadherin ; beta-catenin ; Phalloidin 21362567 24523903 27399334
Growth inhibition assay cell proliferation 24523903
ELISA caspase-9 30320378
In vivo Oral administration of Y-27632 2HCl at 30 mg/kg significantly decreases the blood pressure in a dose-dependent manner in spontaneous hypertensive rats, renal hypertensive rats, as well as deoxycorticosterone acetate (DOCA)-salt hypertensive rats. [1] When Y-27632 2HCl is continuously administered at a rate of 0.55 μL per hour by implanted pumps for 11 days tumor cell invasion (MM1 cells expressing Val14-RhoA in rats) is significantly delayed. [2] By inhibiting ROCK, Y-27632 2HCl treatment attenuates hypoxia-induced angiogenesis and vascular remodeling in the pulmonary circulation. [5] Pretreatment with Y-27632 has a protective effect against tumor formation in albino mice with Ehrlich ascites carcinoma. [7]

Protocol (from reference)

Animal Research:[1] [7]
  • Animal Models: Male Wistar rats with spontaneous or induced hypertension; Swiss albino mice with Ehrlich ascites carcinoma
  • Dosages: 30 mg/kg/day (Rat); 0-10 mg/kg (mice)
  • Administration: Orally (Rat); i.p. (Mice)

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
saline
For best results, use promptly after mixing.

10mg/mL

Chemical Information

Molecular Weight 320.26
Formula

C14H21N3O.2HCl

CAS No. 129830-38-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C1CCC(CC1)C(=O)NC2=CC=NC=C2)N.Cl.Cl

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Frequently Asked Questions

Question 1:
Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

Answer:
The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

Question 2:
Could this product be used in cell culture? Do you have any reference for this application?

Answer:
Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

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