Y-27632 2HCl

Catalog No.S1049

For research use only.

Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.

Y-27632 2HCl Chemical Structure

CAS No. 129830-38-2

Selleck's Y-27632 2HCl has been cited by 765 publications

Purity & Quality Control

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Biological Activity

Description Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.
Targets
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
In vitro

Y-27632 2HCl inhibits ROCK-II while displaying little activity against PKC, cAMP-dependent protein kinase and myosin light-chain kinase (MLCK) with Ki of 26 μM, 25 μM and > 250 μM, respectively, as well as PKA activated by another Rho-family GTPase member, Cdc42. Y-27632 2HCl inhibits smooth-muscle contraction induces by various agonists including phenylephrine, histamine, acetylcholine, serotonin, endothelin, and thromboxane with IC50 of 0.3-1 μM, by selectively inhibiting Ca2+ sensitization. Y-27632 2HCl suppresses Rho-induced, p160ROCK-mediated formation of stress fibres in cultured cells. [1] Y-27632 2HCl treatment blocks both Rho-mediated activation of actomyosin and LPA-stimulated invasive activity of MM1 cells in a concentration-dependent manner. [2] Y-27632 2HCl treatment is not only sufficient to initiate formation of exuberant axonal processes but also facilitates axonal maturation during the very early stages of axonogenesis, while largely sparing axon elongation. [3] In human embryonic stem (hES) cells, Y-27632 2HCl treatment at 10 μM markedly diminishes dissociation-induced apoptosis even in serum-free suspension (SFEB) culture, increases cloning efficiency (from ~1% to ~27%), facilitates subcloning after gene transfer, and enables SFEB-cultured hES cells to survive and differentiate into Bf1+ cortical and basal telencephalic progenitors. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Salivary gland stem cells NHjYRW9HfW6ldHnvckBCe3OjeR?= NVvHbW5FOTBiwsXN M2TCVlch\A>? NVPjeJNFWmWmdXPld{BUT1OFIIPlcoV{[2WwY3W= M1PsOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEC0OVYxLz5{NUiwOFU3ODxxYU6=
HT22 M1zrXWN6fG:2b4jpZ{BCe3OjeR?= MYCxNEDDvU1? NFToUXQyOyCq NFLKPFFRem:2ZXP0d{Bi\2GrboP0JIdtfXSjbXH0[U1qdmS3Y3XkJI5mfXKxbnHsJIRm[XSq NX;SNolKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NVA5OzVpPkKyPFExQDN3PD;hQi=>
Swiss3T3 NG[zfZZEd2yxbomt[o9zdWmwZzDBd5NigQ>? MXexNEDDvU1? NG[z[3gyOyCm NXf5[m5yUW6lcnXhd4V{KHC{b4P0ZZRmKGOnbHygZ49td267LX\vdo1qdmdiYXP0bZZqfHl? MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR4NEmwNkc,OjF2NkS5NFI9N2F-
TSGH 8301 NXLFUZZsVWmpcnH0bY9vKEG|c3H5 MXGyNEDDvU1? NGP3dWgyKGh? MW\JcoNz\WG|ZYOgZ4VtdCCvaXfyZZRqd25? Mnm2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6OU[0O|UoRjF7OEm2OFc2RC:jPh?=
Cynomolgus monkey embryonic stem cells MWrDfZRwfG:6aXOgRZN{[Xl? MXiyNEDDvU1? MlX2NlQhcA>? NILNdHJRem:vb4Tld{BkgUWVIHPlcIwhe3W{dnn2ZYw> MlnQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh7NEC4OVUoRjF6OUSwPFU2RC:jPh?=
PC 12 NXHmeFF1TnWwY4Tpc44hSXO|YYm= NXHobI5rOTEEoN88US=> M3Wye|I1KGh? NEnib2ZCfHSnboXheIV{KGOjdHXjbI9t[W2rbnWgZolwe3mwdHjld4l{ M3H3WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4MkG5OFI1Lz5zNkKxPVQzPDxxYU6=
C2C12 Ml7sSpVv[3Srb36gRZN{[Xl? M1i3OlExyqEQvF2= NYnSUnF[PiCq M1uyc3Bz\X[nboTzJJRp\SC|ZYLpcoUheGixc4Doc5J6dGG2aX;uJI9nKEmUUz2xJIlv\HWlZXSgZpkhcW6|dXzpckBidmRxb4KgWG5HNc7z MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJ4N{GyOEc,OTZ{NkexNlQ9N2F-
Pancreatic acinar cells Ml\3SpVv[3Srb36gRZN{[Xl? M3XSd|ExyqEQvF2= MXu3NEBucW5? MlTSVI91\W62aXH0[ZMhS0ONLYP0bY12dGG2ZXSgdIFv[3KnYYTpZ{Bmdnq7bXWgd4VkemW2aX;u NFOwXpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMke0OVA5OCd-MUK3OFUxQDB:L3G+
Rat hepatic stellate cells NYPGSm9xTnWwY4Tpc44hSXO|YYm= MVezNEDPxE1? NEH6RW01QCCq MoT5SIlucW6rc3jld{B1cGVicHjvd5Bpd3K7bHH0bY9vKG:oIFXyb|ItKGGwZDDk[YNz\WG|ZYOgcoV4KESQQTDzfY51cGW|aYO= M4nZVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyOES1OlY{Lz5zMEi0OVY3OzxxYU6=
LNCaP MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4LVW|I2KM7:TR?= M{\tU|E4KGh? NUDTOYdWTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODd{MES3NUc,OTB5MkC0O|E9N2F-
PC3 NFvSeFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3K0dFI2KM7:TR?= NF3vR3oyPyCq M4LHfWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODd{MES3NUc,OTB5MkC0O|E9N2F-
PC3 NHWzZnJOcWe{YYTpc44hSXO|YYm= M2O4R|I2KM7:TR?= NYGyNYg{OSCq MWjJcohq[mm2czD0bIUhSk2IQj3DUUBidmRidHjlJGVITi2|dHnteYxifGWmIH3p[5JifGmxbh?= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODd{MES3NUc,OTB5MkC0O|E9N2F-
PC3 NX;oN3h4TnWwY4Tpc44hSXO|YYm= MVSyOUDPxE1? MWOxJIg> NYXsc3BmUW6mdXPld{Bud3KyaH;sc4dq[2GuIHPoZY5o\XN? MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODd{MES3NUc,OTB5MkC0O|E9N2F-
Rat Vascular Smooth Muscle Cells NGnFc|ZHfW6ldHnvckBCe3OjeR?= M1u4blExKM7:TR?= M2HEeVIhcA>? NHj2XopKdmirYnn0d{Bidmerb4TlcpNqdiCLST3pcoR2[2WmIHj5dIVzfHKxcHj5 MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODZ2MkOxO{c,OTB4NEKzNVc9N2F-
Stellate Cell M4XT[2Z2dmO2aX;uJGF{e2G7 M1;sPFI2KM7:TR?= MUCxOUBucW5? MVfJcohq[mm2czDmc5Ju[XSrb36gc4YhTi2jY4TpckB{fHKnc4Og[olj\XK|IHHu[EBxcG:|cHjvdplt[XSrb36gc4YhdXmxc3nuJIxq\2i2IHPoZYlv M4PrdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyNkCwOFk3Lz5zME[wNFQ6PjxxYU6=
Neonatal rat ventricular myocytes NHXET3BHfW6ldHnvckBCe3OjeR?= M1\xPFExKM7:TR?= MUe0PEBp MYrJcohq[mm2czDFWE0yNWmwZIXj[YQhcW6lcnXhd4V{KGmwIIDyc5RmcW5ic4nueIhme2m|LDDj[YxtKHOrenWgZY5lKG27b3\pZpJqdGyjcjDvdodidmm8YYTpc44> NWjOV204RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCzPFY3OTNpPkGwN|g3PjF|PD;hQi=>
LS174T MkLIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkW4NVAh|ryP MmL0NVgh\A>? M4P3O21w\GW{YYTlcJkhcW6qaXLpeJMh[2WubDDndo94fGh? MmCwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
HCT116 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVGxNEDPxE1? NHL5cGwyQCCm NFzmcG9UfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? NGrGeVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
HCT15 M2rvTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUTTZWEzOTBizszN NX;KO2gzOThiZB?= NXu5e2xCTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? M{TJelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
SW620 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml:zNVAh|ryP M{jQdVE5KGR? M375c2Rw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NWrlW2Q4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Src-2 NWfhTJc4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkTzNVAh|ryP MYixPEBl NVr4VXVPTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? M2\U[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
Src-1 NH31ZY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjid20yOCEQvF2= M3PlflE5KGR? NGLP[ohFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MojaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
NIH3T3 NXzId|NuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV[xNEDPxE1? M2jWWlE5KGR? Mom3SI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> MljIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
Src-4 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF73NGUyOCEQvF2= M4nUbVE5KGR? MkG2SI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
Src-1 NVPadpM6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVz2V4FLOTBizszN M4nUNlE5KGR? NGS0cmFFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MmK1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
Ras-4 M3HPUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4C1U|ExKM7:TR?= NUfSZmtqOThiZB?= NGe0NWhUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? M2jldlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
Ras-2 NVTnU5BST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPi[WwyOCEQvF2= MXyxPEBl M4TJVnN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
mNET1-e MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXtdGYyOCEQvF2= NIfVU5oyQCCm NXfJV4V[W3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? M1\0fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
mNET1-d NE\0RmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXP4SIJROTBizszN M{fBVlE5KGR? NInRUo1UfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? NWi1WGRnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Dbl-e NYjVdZRMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4\kWlExKM7:TR?= M1qwfVE5KGR? NFzJNm9Od2SncnH0[Yx6KGmwaHnibZR{KGOnbHyg[5Jwf3Sq MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
Dbl-d NGKzbI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVixNEDPxE1? M3zqV|E5KGR? Mn;JV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> NH;ZdIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
NIH3T3 NUPhdoJwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7vWoEzOTBizszN M2LGdVE5KGR? MXTEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
Mesothelial cells from rat mesentery M3z2XmlvfmG|aY\lJGF{e2G7 MXezNEDPxE1? NHvhO2IzOCCq M13uOGJtd2OtczDpcpZie2m4ZTDhZ5Rqfmm2eR?= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86QTNyOEeyK|46QTNyOEeyQE9iRg>?
CCL39 M{\y[GZ2dmO2aX;uJGF{e2G7 MVqzNEDPxE1? MWezNEBucW5? MV3Dc41xdGW2ZXz5JIFjd2yrc3jld{Bi[3SrdnH0bY9vKG:oIF7hMWgh\XilaHHu[4VzKE6KRUGgZpkhcW62ZXfybY5{ MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86Pjl|M{iyK|46Pjl|M{iyQE9iRg>?
HeLa MlTUSpVv[3Srb36gRZN{[Xl? NFTZVlcyOCEQvF2= Mn6xN|AhdWmw M1L3WWlvcGmkaYTzJJRp\SCob4LtZZRqd25ib3[gd5Rz\XO|IH\pZoVzeyCjbnSgeIhmKGG|c3XtZox6KG:oII\pcoN2dGmwLXPvcpRicW6rbneg[o9k[WxiYXTo[ZNqd26| NVvWN2pwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOU[2PFA4Oid-OU[2PFA4OjxxYU6=
N1E-115 MkT5SpVv[3Srb36gRZN{[Xl? MXixNEDPxE1? MmDaNkBp M4DZ[mROW09? NUHuS3Z7UW6qaXLpeJMhfGinIHHzd4Vu[my7IH;mJI1q[3KxdIXieYxmeyCjbnSgbY51\XKvZXTpZZRmKG[rbHHt[Y51eyC2bzDmc5JuKGW6dHXu[IVlKHC{b3Pld5Nmew>? NULrfYVwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOU[0O|Y2PCd-OU[0O|Y2PDxxYU6=
Swiss 3T3 cells MU\GeY5kfGmxbjDBd5NigQ>? NX;ZTIFCOTBizszN NVH1UGNwOiCq NIDW[5RFVVOR NFjLXotKdmirYnn0d{B1cGViYYPz[Y1jdHlib3[gcYlkem:2dXL1cIV{KGGwZDDpcpRmem2nZHnheIUh\mmuYX3lcpR{KHSxIH\vdo0h\Xi2ZX7k[YQheHKxY3Xzd4V{ NX;aVIhkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOU[0O|Y2PCd-OU[0O|Y2PDxxYU6=
Assay
Methods Test Index PMID
Western Blot p-LIMK1(Thr508) ; p-LIMK2(Thr505) ; p-Cofilin(Ser3) ; CDK2 ; KRT7 ; ROCK2 ; E-cadherin ; p-MLC2(Ser19) 24704720 26555939 27399334
Transwell migration assay cell migration inhibition 27694793
Immunofluorescence Neurotoxicity Assay ; E-cadherin ; beta-catenin ; Phalloidin 21362567 24523903 27399334
Growth inhibition assay cell proliferation 24523903
ELISA caspase-9 30320378
In vivo Oral administration of Y-27632 2HCl at 30 mg/kg significantly decreases the blood pressure in a dose-dependent manner in spontaneous hypertensive rats, renal hypertensive rats, as well as deoxycorticosterone acetate (DOCA)-salt hypertensive rats. [1] When Y-27632 2HCl is continuously administered at a rate of 0.55 μL per hour by implanted pumps for 11 days tumor cell invasion (MM1 cells expressing Val14-RhoA in rats) is significantly delayed. [2] By inhibiting ROCK, Y-27632 2HCl treatment attenuates hypoxia-induced angiogenesis and vascular remodeling in the pulmonary circulation. [5] Pretreatment with Y-27632 has a protective effect against tumor formation in albino mice with Ehrlich ascites carcinoma. [7]

Protocol (from reference)

Animal Research:[1] [7]
  • Animal Models: Male Wistar rats with spontaneous or induced hypertension; Swiss albino mice with Ehrlich ascites carcinoma
  • Dosages: 30 mg/kg/day (Rat); 0-10 mg/kg (mice)
  • Administration: Orally (Rat); i.p. (Mice)
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 64 mg/mL warmed
(199.83 mM)
Water 14 mg/mL
(43.71 mM)
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
saline
For best results, use promptly after mixing.

10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 320.26
Formula

C14H21N3O.2HCl

CAS No. 129830-38-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C1CCC(CC1)C(=O)NC2=CC=NC=C2)N.Cl.Cl

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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Frequently Asked Questions

Question 1:
Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

Answer:
The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

Question 2:
Could this product be used in cell culture? Do you have any reference for this application?

Answer:
Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

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