Y-27632

Name: Y-27632
Brand: Selleck Chemicals
SKU: S6390
Rating: 5 (26 reviews)

For research use only.

Catalog No.S6390

26 publications

CAS No. 146986-50-7

Y-27632 is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.

Selleck's Y-27632 has been cited by 26 publications

Sci Adv, 2021, 7(33)eabf4416

PubMed: 34380613 ( click the link to review the publication )

Ocul Surf, 2021, 22:213-223

PubMed: 34419637 ( click the link to review the publication )

Nat Commun, 2021, 12(1):5315

PubMed: 34493720 ( click the link to review the publication )

Nat Commun, 2021, 12(1):4580

PubMed: 34321477 ( click the link to review the publication )

Genome Med, 2021, 13(1):142

PubMed: 34470667 ( click the link to review the publication )

Genome Med, 2021, 13(1):116

PubMed: 34271981 ( click the link to review the publication )

Stem Cell Res, 2021, 55:102455

PubMed: 34352619 ( click the link to review the publication )

Stem Cell Res, 2021, 56:102532

PubMed: 34509917 ( click the link to review the publication )

Stem Cell Res, 2021, 55:102505

PubMed: 34438319 ( click the link to review the publication )

Acta Biomater, 2021, S1742-7061(21)00586-9

PubMed: 34492369 ( click the link to review the publication )

FASEB J, 2021, 35(9):e21799

PubMed: 34339055 ( click the link to review the publication )

Cell Commun Signal, 2021, 19(1):95

PubMed: 34530870 ( click the link to review the publication )

Stem Cell Rev Rep, 2021, 10.1007/s12015-021-10232-9

PubMed: 34415483 ( click the link to review the publication )

Int J Mol Sci, 2021, 22(15)8224

PubMed: 34360992 ( click the link to review the publication )

ACS Biomater Sci Eng, 2021, 7(9):4602-4613

PubMed: 34365789 ( click the link to review the publication )

Sci Rep, 2021, 11(1):17100

PubMed: 34429452 ( click the link to review the publication )

Cell Transplant, 2021, 30:9636897211017830

PubMed: 34053246 ( click the link to review the publication )

Hum Cell, 2021, 10.1007/s13577-021-00612-1

PubMed: 34535876 ( click the link to review the publication )

Methods Mol Biol, 2021, 2319:51-60

PubMed: 34331242 ( click the link to review the publication )

Cell Regen, 2021, 10(1):30

PubMed: 34487238 ( click the link to review the publication )

Theranostics, 2020, 10(24):11215-11229

PubMed: 33042279 ( click the link to review the publication )

J Mol Med (Berl), 2018, 96(2):119-133

PubMed: 29098317 ( click the link to review the publication )

Sci Signal, 2016, 9(450):rs12

PubMed: 27811184 ( click the link to review the publication )

Neurochem Res, 2016, 41(9):2457-69

PubMed: 27216618 ( click the link to review the publication )
Haematologica, 2015, 100(2):157-66

PubMed: 25381126 ( click the link to review the publication )

J Biol Chem, 2015, 290(20):12463-73

PubMed: 25770213 ( click the link to review the publication )

Purity & Quality Control

Choose Selective ROCK Inhibitors

Biological Activity

Description

Targets

ROCK1 ^[1] (Cell-free assay)	ROCK2 ^[1] (Cell-free assay)
140 nM(Ki)	300 nM(Ki)

Protocol

References

[1] M Uehata, et al. Nature. 1997 Oct 30;389(6654):990-4.

Solubility (25°C)

In vitro	DMSO	49 mg/mL (198.1 mM)
	Water	Insoluble
	Ethanol	''49 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	247.34
Formula	C₁₄H₂₁N₃O
CAS No.	146986-50-7
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

ROCK Signaling Pathway Map

Related ROCK Products

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ML141 ^New

ML141 (CID-2950007) is demonstrated to be a potent, selective and reversible non-competitive inhibitor of Cdc42 GTPase suitable for in vitro assays, with IC50 of 200 nM and selectivity against other members of the Rho family of GTPases (Rac1, Rab2, Rab7). ML141 is associated with an increase in p38 activation and may induce p38-dependent apoptosis/senescence. ML141 also protects neuroblastoma cells from metformin-induced apoptosis.
Salirasib ^New

Salirasib (Farnesylthiosalicylic acid, FTS) is a potent competitive prenylated protein methyltransferase (PPMTase) inhibitor with K_i of 2.6 μM, which inhibits _Ras methylation. Salirasib exerts antitumor effects and induces autophagy. Salirasib exerts antitumor effects and induces autophagy. Phase 2.
Y-27632 2HCl

Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with K_i of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.
Thiazovivin

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GSK429286A

GSK429286A (RHO-15) is a selective inhibitor of ROCK1 and ROCK2 with IC50 of 14 nM and 63 nM, respectively.

Features:Greater selectivity than Y-27632.
Fasudil (HA-1077) HCl

Fasudil (HA-1077, AT-877), a potent and selective inhibitor of Rho kinase, displays less potent inhibiton over PKA, PKG, PKC and MLCK with Ki of 1.6, 1.6, 3.3, and 36 μM in cell-free assays, respectively. Fasudil is also a calcium channel blocker.
Palbociclib (PD-0332991) HCl

Palbociclib (PD-0332991) HCl is a highly selective inhibitor of CDK4/6 with IC50 of 11 nM/16 nM in cell-free assays, respectively. It shows no activity against CDK1/2/5, EGFR, FGFR, PDGFR, InsR, etc. Phase 3.

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Alisertib (MLN8237)

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Features:First orally available inhibitor of Aurora A.
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Features:The first reported Wee1 inhibitor.

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Y-27632