Y-27632 2HCl

Catalog No.S1049

Y-27632 2HCl Chemical Structure

Molecular Weight(MW): 320.26

Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.

Size Price Stock Quantity  
In DMSO USD 156 In stock
USD 70 In stock
USD 120 In stock
USD 170 In stock
USD 570 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 359 Publications

Purity & Quality Control

Choose Selective ROCK Inhibitors

Biological Activity

Description Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.
Targets
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
In vitro

Y-27632 2HCl inhibits ROCK-II while displaying little activity against PKC, cAMP-dependent protein kinase and myosin light-chain kinase (MLCK) with Ki of 26 μM, 25 μM and > 250 μM, respectively, as well as PKA activated by another Rho-family GTPase member, Cdc42. Y-27632 2HCl inhibits smooth-muscle contraction induces by various agonists including phenylephrine, histamine, acetylcholine, serotonin, endothelin, and thromboxane with IC50 of 0.3-1 μM, by selectively inhibiting Ca2+ sensitization. Y-27632 2HCl suppresses Rho-induced, p160ROCK-mediated formation of stress fibres in cultured cells. [1] Y-27632 2HCl treatment blocks both Rho-mediated activation of actomyosin and LPA-stimulated invasive activity of MM1 cells in a concentration-dependent manner. [2] Y-27632 2HCl treatment is not only sufficient to initiate formation of exuberant axonal processes but also facilitates axonal maturation during the very early stages of axonogenesis, while largely sparing axon elongation. [3] In human embryonic stem (hES) cells, Y-27632 2HCl treatment at 10 μM markedly diminishes dissociation-induced apoptosis even in serum-free suspension (SFEB) culture, increases cloning efficiency (from ~1% to ~27%), facilitates subcloning after gene transfer, and enables SFEB-cultured hES cells to survive and differentiate into Bf1+ cortical and basal telencephalic progenitors. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Swiss 3T3 cells NXLFfXRHTnWwY4Tpc44hSXO|YYm= NXfwSopoOTBizszN MmrmNkBp NH\FdIVFVVOR NWfUTlZ{UW6qaXLpeJMhfGinIHHzd4Vu[my7IH;mJI1q[3KxdIXieYxmeyCjbnSgbY51\XKvZXTpZZRmKG[rbHHt[Y51eyC2bzDmc5JuKGW6dHXu[IVlKHC{b3Pld5Nmew>? NX2xSox6QTZ2N{[1OC=>
N1E-115 MmDCSpVv[3Srb36gRZN{[Xl? NELoOWMyOCEQvF2= Ml:0NkBp NFH1e4xFVVOR NH[0dINKdmirYnn0d{B1cGViYYPz[Y1jdHlib3[gcYlkem:2dXL1cIV{KGGwZDDpcpRmem2nZHnheIUh\mmuYX3lcpR{KHSxIH\vdo0h\Xi2ZX7k[YQheHKxY3Xzd4V{ NIPUZZg6PjR5NkW0
HeLa MnXQSpVv[3Srb36gRZN{[Xl? Mnv6NVAh|ryP MnTuN|AhdWmw NGfB[HRKdmirYnn0d{B1cGViZn;ycYF1cW:wIH;mJJN1emW|czDmbYJmenNiYX7kJJRp\SCjc4PlcYJtgSCxZjD2bY5kfWyrbj3jc451[WmwaX7nJIZw[2GuIHHkbIV{cW:wcx?= NIr2eVU6PjZ6MEey
CCL39 MlP3SpVv[3Srb36gRZN{[Xl? NXPmRXl6OzBizszN NWDOeYxqOzBibXnu NVnlSWRlS2:vcHzleIVtgSCjYn;sbZNp\XNiYXP0bZZifGmxbjDv[kBP[S2KIHX4Z4hidmencjDOTGUyKGK7IHnueIVoemmwcx?= NFHoUZI6Pjl|M{iy
Mesothelial cells from rat mesentery NF3nRZdKdn[jc3n2[UBCe3OjeR?= Mon1N|Ah|ryP NYLIeYQzOjBiaB?= M{H4SGJtd2OtczDpcpZie2m4ZTDhZ5Rqfmm2eR?= MWC5PVMxQDd{
NIH3T3 NW\zTpJbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nVe|ExKM7:TR?= M4rlTlE5KGR? M2fTbWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MmDvNVAxOjF|OE[=
Dbl-d MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUTLZYNTOTBizszN M4L1R|E5KGR? NHfGbZZUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? Mn:0NVAxOjF|OE[=
Dbl-e MoroS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVexNEDPxE1? M1vwNVE5KGR? NEjDZYlOd2SncnH0[Yx6KGmwaHnibZR{KGOnbHyg[5Jwf3Sq MmXJNVAxOjF|OE[=
mNET1-d NVy4cYhxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWWxNEDPxE1? NV;zflVyOThiZB?= NYLHcZdTW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? NUL0emVTOTByMkGzPFY>
mNET1-e NGiweG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWmxNEDPxE1? MnnXNVgh\A>? M3;yN3N1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp NHW2dIgyODB{MUO4Oi=>
Ras-2 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPhdG9iOTBizszN M2T5[|E5KGR? NGrrcoJUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? MUSxNFAzOTN6Nh?=
Ras-4 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfS[|NEOTBizszN MYixPEBl MWLTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> MlPJNVAxOjF|OE[=
Src-1 NELuZ3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF70RYoyOCEQvF2= M1zqPFE5KGR? NFj6cVZFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MXuxNFAzOTN6Nh?=
Src-4 M2r1XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnETJFNOTBizszN NXfYbHFLOThiZB?= M1SySmRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> M4L2UFExODJzM{i2
NIH3T3 NH7FNGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX\UUm1vOTBizszN NXXL[2d3OThiZB?= NHPDXldFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MlnaNVAxOjF|OE[=
Src-1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojnNVAh|ryP NVzofpFROThiZB?= NW\VcWh5TG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MXWxNFAzOTN6Nh?=
Src-2 NVfsR5FVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\ONVAh|ryP NYfQSWh3OThiZB?= NIHGUVRFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NXLxUVN1OTByMkGzPFY>
SW620 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;KW2VMOTBizszN MVmxPEBl NXq0b2gzTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MVWxNFAzOTN6Nh?=
HCT15 MnS5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYexNEDPxE1? NHXldFgyQCCm NF;1OWdFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NEHJ[pQyODB{MUO4Oi=>
HCT116 NYe3O3lkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUixNEDPxE1? NV7OTHpbOThiZB?= M2fucXN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp M{TyPVExODJzM{i2
LS174T MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX6xNEDPxE1? MVexPEBl MVnNc4RmemG2ZXz5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp MnW3NVAxOjF|OE[=
Neonatal rat ventricular myocytes NYXOdpdMTnWwY4Tpc44hSXO|YYm= MVixNEDPxE1? NGTtZWo1QCCq NFnpUpRKdmirYnn0d{BGXC1zLXnu[JVk\WRiaX7jdoVie2W|IHnuJJBzd3SnaX6gd5lvfGinc3nzMEBk\WyuIIPpfoUh[W6mIH35c4Zq[nKrbHzhdkBwemejbnn6ZZRqd25? M3XtVlExOzh4NkGz
Stellate Cell MYnGeY5kfGmxbjDBd5NigQ>? M3PqOVI2KM7:TR?= NIDnZoYyPSCvaX6= MU\Jcohq[mm2czDmc5Ju[XSrb36gc4YhTi2jY4TpckB{fHKnc4Og[olj\XK|IHHu[EBxcG:|cHjvdplt[XSrb36gc4YhdXmxc3nuJIxq\2i2IHPoZYlv MUCxNFYxODR7Nh?=
Rat Vascular Smooth Muscle Cells NVHzOo16TnWwY4Tpc44hSXO|YYm= NHryTI0yOCEQvF2= M3vtN|IhcA>? NV3VUY5CUW6qaXLpeJMh[W6paX;0[Y5{cW5iSVmtbY5lfWOnZDDofZBmenS{b4DofS=> NVjC[5dYOTB4NEKzNVc>
PC3 MoH4SpVv[3Srb36gRZN{[Xl? NYHNbGRbOjVizszN MXOxJIg> Mmm3TY5lfWOnczDtc5JxcG:ub3fpZ4FtKGOqYX7n[ZM> MYixNFczODR5MR?=
PC3 NHLqS3BOcWe{YYTpc44hSXO|YYm= NF;iVGQzPSEQvF2= NXrJcHVnOSCq NVvE[W43UW6qaXLpeJMhfGinIFLNSmIuS01iYX7kJJRp\SCHR1[td5RqdXWuYYTl[EBucWe{YYTpc44> M2joTlExPzJyNEex
PC3 M1uzS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jLVFI2KM7:TR?= MV:xO{Bp MlXQSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> MnvwNVA4OjB2N{G=
LNCaP MkOwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFqxVWszPSEQvF2= M1jkcVE4KGh? NEPuRm9Fd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NWrnfJlGOTB5MkC0O|E>
Rat hepatic stellate cells MU\GeY5kfGmxbjDBd5NigQ>? MoS1N|Ah|ryP NELC[Xk1QCCq Mo\QSIlucW6rc3jld{B1cGVicHjvd5Bpd3K7bHH0bY9vKG:oIFXyb|ItKGGwZDDk[YNz\WG|ZYOgcoV4KESQQTDzfY51cGW|aYO= NGO4cGwyODh2NU[2Ny=>
Pancreatic acinar cells Ml\BSpVv[3Srb36gRZN{[Xl? NVXsbWFVOTEEoN88US=> MX63NEBucW5? MnfVVI91\W62aXH0[ZMhS0ONLYP0bY12dGG2ZXSgdIFv[3KnYYTpZ{Bmdnq7bXWgd4VkemW2aX;u MVixNlc1PTB6MB?=
C2C12 MojpSpVv[3Srb36gRZN{[Xl? NHzE[5UyOMLizszN MWi2JIg> NFXDO4FRemW4ZX70d{B1cGVic3XybY5mKHCqb4PwbI9zgWyjdHnvckBw\iCLUmOtNUBqdmS3Y3XkJIJ6KGmwc4XsbY4h[W6mL3;yJHRPTi4QsR?= NX;ORVFTOTZ{NkexNlQ>
PC 12 M4PQZ2Z2dmO2aX;uJGF{e2G7 M4rHR|ExyqEQvF2= MlnpNlQhcA>? MnK1RZR1\W63YYTld{Bk[XSnY3jvcIFucW6nIHLpc5N6dnSqZYPpdy=> M2X3XlE3OjF7NEK0
Cynomolgus monkey embryonic stem cells NF31dFFEgXSxdH;4bYMhSXO|YYm= MXmyNEDDvU1? NHHKVGczPCCq MYLQdo9ud3SnczDjfWVUKGOnbHygd5Vzfmm4YXy= M3jzNFE5QTRyOEW1
TSGH 8301 MlHIUYloemG2aX;uJGF{e2G7 Ml\INlAhyrWP M{TNclEhcA>? MlXTTY5kemWjc3XzJINmdGxibXnndoF1cW:w MlHCNVk5QTZ2N{W=
Swiss3T3 MXHDc4xwdnlvZn;ycYlv\yCDc4PhfS=> Mn;QNVAhyrWP MlHPNVMh\A>? MWrJcoNz\WG|ZYOgdJJwe3SjdHWgZ4VtdCClb3zvcpku\m:{bXnu[{Bi[3Srdnn0fS=> NVj1UlNoOjF2NkS5NFI>
HT22 MkfwR5l1d3SxeHnjJGF{e2G7 NVLhN|FzOTBiwsXN Mmr3NVMhcA>? MV3Qdo91\WO2czDh[4FqdnO2IHfseZRidWG2ZT3pcoR2[2WmIH7leZJwdmGuIHTlZZRp MlHuNlI5OTB6M{W=
Salivary gland stem cells MkPDSpVv[3Srb36gRZN{[Xl? NGjZeZUyOCEEtV2= MXq3JIQ> M2C3fHJm\HWlZYOgV2dUSyC|ZX7ld4NmdmOn MWqyOVgxPDV4MB?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western Blot
p-LIMK1(Thr508); 

PubMed: 24704720     


Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.

p-LIMK2(Thr505); 

PubMed: 24704720     


Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.

p-Cofilin(Ser3); 

PubMed: 24704720     


Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.

CDK2; 

PubMed: 26555939     


Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells cultured without Y-27632 in the same condition are used as control. β-ACTIN serves as an endogenous control. 

KRT7; 

PubMed: 26555939     


Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells cultured without Y-27632 in the same condition are used as control. β-ACTIN serves as an endogenous control. 

ROCK2; 

PubMed: 26555939     


Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells cultured without Y-27632 in the same condition are used as control. β-ACTIN serves as an endogenous control. 

E-cadherin; 

PubMed: 27399334     


The expression and/or phosphorylation of E-cadherin and ROCKs downstream targets in SW-480-FOXM1D cells treated with Y-27632.

p-MLC2(Ser19); 

PubMed: 27399334     


The expression and/or phosphorylation of E-cadherin and ROCKs downstream targets in SW-480-FOXM1D cells treated with Y-27632.

24704720 26555939 27399334
Transwell migration assay
cell migration inhibition ; 

PubMed: 27694793     


Data represent mean ± SEM, n=3. Compared with control, * P<0.05, ** P<0.01. Compared with control, Y-27632, or EGCG, *** P<0.01.

27694793
Immunofluorescence
Neurotoxicity Assay; 

PubMed: 21362567     


Representations of neurodegeneration in H9-, HUF5- and G2019S-iPSC derived neurons when treated with neurotoxins, H2O2 and MG-132, and ROCK inhibitor Y-27632. Scale bar = 50 µm.

E-cadherin; 

PubMed: 24523903     


The localized patterns of E-cadherin and b-catenin were examined using specific antibodies through confocal laser microscopy. MCF-7 cells were cultured for 24 hrs in the absence or presence of Y-27632 (20 µM) to inhibit ROCK activity. 

beta-catenin; 

PubMed: 24523903     


The localized patterns of E-cadherin and b-catenin were examined using specific antibodies through confocal laser microscopy. MCF-7 cells were cultured for 24 hrs in the absence or presence of Y-27632 (20 µM) to inhibit ROCK activity. 

Phalloidin; 

PubMed: 27399334     


Triple IF staining for F-actin (phalloidin, green) E-cadherin (b) (red) and nuclei (DAPI, blue) showed decreased F-actin, increased E-cadherin and altered cell shape and size in SW-480-FOXM1D cells treated with the ROCKs inhibitor Y-27632 or fasudil (both 10 μM, for 24 h). Scale bar, 25 μm. 

21362567 24523903 27399334
Growth inhibition assay
cell proliferation ; 

PubMed: 24523903     


MCF-7 cells and MDA-MB-231 cells were cultured on tissue culture plates for four days with or without Y-27632 (20 µM). Relative cell proliferation rates were determined by the cell number assayed using CCK-8 kit. The data are expressed as the mean ± SD. n = 3 culture dishes. The p-value was less than 0.05 for comparisons between control (Control) and treatment groups (Y-27632) in MCF-7 cells. *, p<0.05.

24523903
ELISA
caspase-9; 

PubMed: 30320378     


Caspase-9 activity was measured via ELISA. Mst1-mediated casapse-9 activation was inhibited by Y-27632 in A549 cells. *P<0.05. Ad, adenovirus; Mst1, mammalian STE20-like kinase 1.

30320378
In vivo Oral administration of Y-27632 2HCl at 30 mg/kg significantly decreases the blood pressure in a dose-dependent manner in spontaneous hypertensive rats, renal hypertensive rats, as well as deoxycorticosterone acetate (DOCA)-salt hypertensive rats. [1] When Y-27632 2HCl is continuously administered at a rate of 0.55 μL per hour by implanted pumps for 11 days tumor cell invasion (MM1 cells expressing Val14-RhoA in rats) is significantly delayed. [2] By inhibiting ROCK, Y-27632 2HCl treatment attenuates hypoxia-induced angiogenesis and vascular remodeling in the pulmonary circulation. [5] Pretreatment with Y-27632 has a protective effect against tumor formation in albino mice with Ehrlich ascites carcinoma. [7]

Protocol

Animal Research:[1] [7]
- Collapse
  • Animal Models: Male Wistar rats with spontaneous or induced hypertension; Swiss albino mice with Ehrlich ascites carcinoma
  • Formulation: Dissolved in DMSO, and diluted in saline (Rat); 0.9% NaCl (Mice)
  • Dosages: 30 mg/kg/day (Rat); 0-10 mg/kg (mice)
  • Administration: Orally (Rat); i.p. (Mice)
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 64 mg/mL warmed (199.83 mM)
Water 14 mg/mL (43.71 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
saline
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 320.26
Formula

C14H21N3O.2HCl

CAS No. 129830-38-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

  • Answer:

    The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

  • Question 2:

    Could this product be used in cell culture? Do you have any reference for this application?

  • Answer:

    Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

ROCK Signaling Pathway Map

ROCK Inhibitors with Unique Features

Related ROCK Products

Tags: buy Y-27632 2HCl | Y-27632 2HCl supplier | purchase Y-27632 2HCl | Y-27632 2HCl cost | Y-27632 2HCl manufacturer | order Y-27632 2HCl | Y-27632 2HCl distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID