SB216763

Catalog No.S1075

SB216763 Chemical Structure

Molecular Weight(MW): 371.22

SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.

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In DMSO USD 165 In stock
USD 147 In stock
USD 270 In stock
USD 870 In stock
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Cited by 11 Publications

4 Customer Reviews

  • Cancer Res, 2018, doi:10.1158/0008-5472. SB216763 purchased from Selleck.

    Viability assays for five additional GSK3 inhibitors in SY5Y cells.

    Mol Cancer Ther, 2013, 13(2): 454-67 . SB216763 purchased from Selleck.

  • Mol Cancer Ther 2014 13(1), 37-48. SB216763 purchased from Selleck.

    In the presence of SB216763 (2 nM), transwell migration and invasion assay was conducted to evaluate the cell metastasis after Serpine2 transfection. Data were from three independent experiments and were average ± S.E. values. **P < 0.01, compared to transfected vector cells; ##P < 0.01, compared to transfected Serpine2 cells.

    Am J Transl Res, 2016, 8(5):1985-97.. SB216763 purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.
Targets
GSK-3α [1]
(Cell-free assay)
GSK-3β [1]
(Cell-free assay)
34.3 nM ~34.3 nM
In vitro

SB 216763 displays similar potency for GSK-3β with 96% inhibition at 10 μM while exhibiting minimal activity against 24 other protein kinases including PKBα and PDK1 with IC50 of >10 μM. SB 216763 stimulates glycogen synthesis in human liver cells with EC50 of 3.6 μM, and induces dose-dependent transcription of a β-catenin-LEF/TCF regulated reporter gene in HEK293 cells with a maximum 2.5-fold induction at 5 μM. [1] SB 216763 protects the cerebellar granule neurones from apoptotic cell death induced by LY-294002 or potassium-deprivation in a concentration-dependent manner, with a maximal neuroprotection at 3 μM in contrast with the effect of lithium chloride at which 10 mM is required. SB 216763 at 3 μM also completely prevents death of chicken dorsal root ganglion sensory neurones induced by LY-294002 regardless of NGF. SB 216763 treatment at 5 μM markedly inhibits the GSK-3-dependent phosphorylation of neuronal-specific microtubule-associated protein tau in cerebellar granule neurones or recombinant tau in HEK293 cells, and induces increased levels of cytoplasmic β-catenin in both cells mimicking the effect of Wnt-mediated inhibition of GSK-3. [2] In pancreatic cancer cell lines including BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC, SB 216763 treatment at 25-50 μM reduces cell viability in a dose-dependent manner, and leads to significant increase in apoptosis by 50% at 72 hours due to the specific down regulation of GSK-3β, while has no effect in HMEC or WI38 cell lines. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells NV\pXJd7TnWwY4Tpc44h[XO|YYm= MlHsS4x6[2:pZX6gd5lvfGijc3WgZYN1cX[rdImgc4YhUEWNMkmzJINmdGy|LDDpcohq[mm2aX;uJI9nKEeVS{OtZoV1[SxiRVO1NF0xNjJizszN NUXXZnV1OTJ7NEGzN|E>
human HDLM-2 cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUTJcohq[mm2aX;uJI9nKGi3bXHuJGhFVE1vMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNlA6QDRizszN MVHTRW5ITVJ?
human NCI-H1770 cell M{LO[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFrafoxKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVc4OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LjDJR|UxRTBwMkmzPVIh|ryP NEHJdGdUSU6JRWK=
human HOP-62 cell M1r1SWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NY\HUIJuUW6qaXLpeIlwdiCxZjDoeY1idiCKT2CtOlIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjh3MkW2JO69VQ>? NWfGb445W0GQR1XS
human JAR cell NHnJNVJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{nNUWlvcGmkaYTpc44hd2ZiaIXtZY4hUkGUIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6yNVA1PCEQvF2= MWDTRW5ITVJ?
human K5 cell NHnVOZFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn3YTY5pcWKrdHnvckBw\iCqdX3hckBMPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5MEBKSzVyPUGuOVM3QThizszN NUHqUI1QW0GQR1XS
human MV-4-11 cell MnrMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYrndoU5UW6qaXLpeIlwdiCxZjDoeY1idiCPVj20MVEyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi54OU[4OUDPxE1? NX7kT|Z3W0GQR1XS
human BT-549 cell NXLyVWtET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXXJcohq[mm2aX;uJI9nKGi3bXHuJGJVNTV2OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuNlk{PzZizszN NF\4UHdUSU6JRWK=
human LB2241-RCC cell MnvxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV3Jcohq[mm2aX;uJI9nKGi3bXHuJGxDOjJ2MT3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03NjR{OEe0JO69VQ>? MVPTRW5ITVJ?
human GAMG cell NIXLT4dIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUnJcohq[mm2aX;uJI9nKGi3bXHuJGdCVUdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14Lk[1OFA6KM7:TR?= MVrTRW5ITVJ?
human HMV-II cell NFyyR4pIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NE[4OVNKdmirYnn0bY9vKG:oIHj1cYFvKEiPVj3JTUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvPjd4MEeg{txO NUTocW9pW0GQR1XS
human RS4-11 cell NFfrcYNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUXJcohq[mm2aX;uJI9nKGi3bXHuJHJUPC1zMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNlQyPDFizszN NWrhPI5sW0GQR1XS
human NCI-H1993 cell MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWL0ZYFOUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE6QTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16LkO2OVM1KM7:TR?= NILQb|JUSU6JRWK=
human IST-SL1 cell M2\s[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVPJcohq[mm2aX;uJI9nKGi3bXHuJGlUXC2VTEGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlAzODRizszN MXLTRW5ITVJ?
human SK-OV-3 cell MmTRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXGyeJA1UW6qaXLpeIlwdiCxZjDoeY1idiCVSz3PWk0{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QS5zMECyJO69VQ>? M1XTW3NCVkeHUh?=
human ESS-1 cell M1u1XGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmPvTY5pcWKrdHnvckBw\iCqdX3hckBGW1NvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuOVk5PzJizszN MkDRV2FPT0WU
human G-361 cell MmDPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3LpdGlvcGmkaYTpc44hd2ZiaIXtZY4hTy1|NkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE4yPzl6IN88US=> NFK1blBUSU6JRWK=
human ALL-PO cell NYLZNFRCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWLJcohq[mm2aX;uJI9nKGi3bXHuJGFNVC2STzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlM1PzdizszN M3TUXXNCVkeHUh?=
human NB5 cell NXT3UW9NT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUfmUVBVUW6qaXLpeIlwdiCxZjDoeY1idiCQQkWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4zOTV5IN88US=> M4K5ZnNCVkeHUh?=
human DU-145 cell M{PCSmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXexblVqUW6qaXLpeIlwdiCxZjDoeY1idiCGVT2xOFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOS54NUO1JO69VQ>? M13qfHNCVkeHUh?=
human NMC-G1 cell NFPUcIFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUnE[GdIUW6qaXLpeIlwdiCxZjDoeY1idiCQTVOtS|Eh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOS55Mk[0JO69VQ>? NIDPZ4ZUSU6JRWK=
human RPMI-7951 cell NVPoRopPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mo\4TY5pcWKrdHnvckBw\iCqdX3hckBTWE2LLUe5OVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigS5iSVO1NF0yOS55OE[0JO69VQ>? NYrWVpNIW0GQR1XS
human NCI-H2009 cell M33tWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{DVcmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFA6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTNwMkSyO{DPxE1? M4[5ZnNCVkeHUh?=
human LoVo cell MYDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV\rVGRUUW6qaXLpeIlwdiCxZjDoeY1idiCOb2\vJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvOzd5MTFOwG0> NI\6ZYdUSU6JRWK=
human A2058 cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXTXcmlZUW6qaXLpeIlwdiCxZjDoeY1idiCDMkC1PEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{NjR4OTFOwG0> MYHTRW5ITVJ?
human D-566MG cell M1rUbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MonVTY5pcWKrdHnvckBw\iCqdX3hckBFNTV4Nl3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvQTR6MTFOwG0> M1j2bHNCVkeHUh?=
human QIMR-WIL cell NWLjfFRsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUTmfHZ2UW6qaXLpeIlwdiCxZjDoeY1idiCTSV3SMXdKVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTF3Lk[zPVQh|ryP MonTV2FPT0WU
human S-117 cell NGrrb5RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmrBTY5pcWKrdHnvckBw\iCqdX3hckBUNTFzNzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG2MlQxOjJizszN NWK2dIhlW0GQR1XS
human YH-13 cell NWPTXm9vT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUnEeWZsUW6qaXLpeIlwdiCxZjDoeY1idiC\SD2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE3NjZ6NkWg{txO MYDTRW5ITVJ?
human IGROV-1 cell NFr6W5NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGjjS2pKdmirYnn0bY9vKG:oIHj1cYFvKEmJUl;WMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPy5{MECxJO69VQ>? M1TjbXNCVkeHUh?=
human BHT-101 cell NHjpcohIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVLXdHp{UW6qaXLpeIlwdiCxZjDoeY1idiCESGStNVAyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTdwNkC5PUDPxE1? NUi5V2pMW0GQR1XS
human MKN45 cell M{fxUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXrLUGgxUW6qaXLpeIlwdiCxZjDoeY1idiCPS160OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE5NjRzMkig{txO MVrTRW5ITVJ?
human A498 cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnewTY5pcWKrdHnvckBw\iCqdX3hckBCPDl6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUmuOFU1QSEQvF2= NHf3O|VUSU6JRWK=
human U-266 cell NFexPIFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUjjbIx6UW6qaXLpeIlwdiCxZjDoeY1idiCXLUK2OkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIxNjh5OUeg{txO M2LHeHNCVkeHUh?=
human BFTC-905 cell M{n2dmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmHaTY5pcWKrdHnvckBw\iCqdX3hckBDTlSFLUmwOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIyNjF6N{mg{txO MmnYV2FPT0WU
human BxPC-3 cell MmjGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NILmfFhKdmirYnn0bY9vKG:oIHj1cYFvKEK6UFOtN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIyNjN3NUSg{txO NF;YcIVUSU6JRWK=
human NCI-SNU-1 cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmLUTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvU17VMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOS53NUW4JO69VQ>? M4q4bXNCVkeHUh?=
human SF539 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MV3Jcohq[mm2aX;uJI9nKGi3bXHuJHNHPTN7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkKuNFE6QCEQvF2= NV[2V|l2W0GQR1XS
human VA-ES-BJ cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIrUNW9KdmirYnn0bY9vKG:oIHj1cYFvKF[DLVXTMWJLKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjJwNUe2N{DPxE1? NY\lUVBOW0GQR1XS
human HuO-3N1 cell MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH;0S21KdmirYnn0bY9vKG:oIHj1cYFvKEi3Tz2zUlEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOi54MUWxJO69VQ>? MWHTRW5ITVJ?
human EM-2 cell M3Lhbmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1Lo[WlvcGmkaYTpc44hd2ZiaIXtZY4hTU1vMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKyMlY3QDlizszN MluxV2FPT0WU
human T98G cell NFLBWJJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn;mTY5pcWKrdHnvckBw\iCqdX3hckBVQTiJIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkOuN|AxQSEQvF2= M1\CU3NCVkeHUh?=
human SW1783 cell NWPxS4YyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHm4PHJKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUe4N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI{NjV{NEOg{txO MmrWV2FPT0WU
human NKM-1 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUXJcohq[mm2aX;uJI9nKGi3bXHuJG5MVS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkOuPFUyOiEQvF2= NH\IU5NUSU6JRWK=
human KOSC-2 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIq1RllKdmirYnn0bY9vKG:oIHj1cYFvKEuRU1OtNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI1NjF{OUKg{txO MoLWV2FPT0WU
human SW48 cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NICw[HdKdmirYnn0bY9vKG:oIHj1cYFvKFOZNEigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOE43ODZ5IN88US=> MVrTRW5ITVJ?
human MIAPaCa2 cells M{nHbmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXy3NkBp MoLuS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUWlCWGGFYUKgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF0zPSEQvF2= NYT0Tm9[OTl|M{izOVU>
human NCI-H28 cell NETKXplIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFjvdVJKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPS5{MUC0JO69VQ>? NX3V[JRIW0GQR1XS
human 5637 cell MoXUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NF6ze5JKdmirYnn0bY9vKG:oIHj1cYFvKDV4M{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOU45OTFzIN88US=> NV;0dm1UW0GQR1XS
human NB69 cell NE\penlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4PqOWlvcGmkaYTpc44hd2ZiaIXtZY4hVkJ4OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK2MlE4PjRizszN NV22U41GW0GQR1XS
human HT-29 cell MkfMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2jyOWlvcGmkaYTpc44hd2ZiaIXtZY4hUFRvMkmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yO{4yPzV{IN88US=> M4HJ[3NCVkeHUh?=
human PFSK-1 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYn4cnFHUW6qaXLpeIlwdiCxZjDoeY1idiCSRmPLMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPy53OUOg{txO MUHTRW5ITVJ?
human UACC-257 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVvJcohq[mm2aX;uJI9nKGi3bXHuJHVCS0NvMkW3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlcvPzR|IN88US=> M3HuWnNCVkeHUh?=
human D-423MG cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXzWeJJJUW6qaXLpeIlwdiCxZjDoeY1idiCGLUSyN21IKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjdwOUGwOkDPxE1? NFHjXoVUSU6JRWK=
human NH-12 cell MkLQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWrJcohq[mm2aX;uJI9nKGi3bXHuJG5JNTF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkiuOFA2QSEQvF2= M3LFUHNCVkeHUh?=
human DEL cell NWP1U|ZVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Ml;RTY5pcWKrdHnvckBw\iCqdX3hckBFTUxiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{OT6wOFc1KM7:TR?= MWjTRW5ITVJ?
human LS-513 cell MnHwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Ml3DTY5pcWKrdHnvckBw\iCqdX3hckBNWy13MUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zNE4yOzN2IN88US=> MUHTRW5ITVJ?
human NBsusSR cell MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYL1fnBKUW6qaXLpeIlwdiCxZjDoeY1idiCQQoP1d3NTKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzBwNU[3PEDPxE1? NIDvV5RUSU6JRWK=
human BV-173 cell NXn3V45HT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUHC[2FCUW6qaXLpeIlwdiCxZjDoeY1idiCEVj2xO|Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{OC55NkS5JO69VQ>? MV;TRW5ITVJ?
human A101D cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{[0b2lvcGmkaYTpc44hd2ZiaIXtZY4hSTFyMVSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zNE46Pzh2IN88US=> M2\rfHNCVkeHUh?=
human LU-134-A cell NXTa[GpVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{PncmlvcGmkaYTpc44hd2ZiaIXtZY4hVFVvMUO0MWEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{OS5yMkO4JO69VQ>? NV;4eWxoW0GQR1XS
human ES3 cell MkHnS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUPJcohq[mm2aX;uJI9nKGi3bXHuJGVUOyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTNzLkOzO|Yh|ryP M{S4TXNCVkeHUh?=
human NY cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV3mTYhmUW6qaXLpeIlwdiCxZjDoeY1idiCQWTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOyMlI6PjVizszN NH7BUodUSU6JRWK=
human NCI-H1975 cell NX;UdG1oT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVLJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUm3OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMzNjN3OEKg{txO NIe4eYJUSU6JRWK=
human A704 cell MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NY\rRYN4UW6qaXLpeIlwdiCxZjDoeY1idiCDN{C0JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|QvOjB3OTFOwG0> NHPaUZRUSU6JRWK=
human SK-MEL-24 cell M3TSe2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHLJVoFKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0zPCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN2LkK1NlMh|ryP MUnTRW5ITVJ?
human SW1088 cell NUPiUXY2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{jDU2lvcGmkaYTpc44hd2ZiaIXtZY4hW1dzMEi4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|QvOzR3MjFOwG0> NX7pOmYzW0GQR1XS
human HepG2 cells MXLGeY5kfGmxbjDhd5NigQ>? MnS0N|Qh|ryP Mk\tNVIhcA>? MWTJcohq[mm2aX;uJI9nKEeVS{Pi[ZRiKGmwIHj1cYFvKEincFeyJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKHKjdHnvJI9nKHCqb4PwbI9zgWyjdHXkJGdUN0eVIHH0JFM1KHWPIHHmeIVzKDF{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= NWm5TGxCOjV2NkexOVA>
mouse 3T3L1 cells MWPGeY5kfGmxbjDhd5NigQ>? NHL3[5I{PCEQvF2= M1r3XFEzKGh? NGrhTXRKdmO{ZXHz[UBqdiCpbIXjc5NmKGmwY3;ydI9z[XSrb36gbY4hdW:3c3WgN3Q{VDFiY3XscJMh[XRiM{SgeW0h[W[2ZYKgNVIhcHK|IHL5JIdtfWOxc3WgZZN{[Xl? NXfWZWRrOjV2NkexOVA>
human ST14A cells NFjEWHBHfW6ldHnvckBie3OjeR?= NV2yV2I{PiCq NY\H[IN{UW6qaXLpeIlwdiCxZjDHV2suOy2kZYThMY1m\GmjdHXkJHdvfCC|aXfuZYxqdmdiaX6gbJVu[W5iU2SxOGEh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iYXPjeY12dGG2aX;uJI9nKGKndHGtZ4F{\WmwIHHyc5Vv\CCwdXPs[ZV{KGGodHXyJFYhcHK|IHL5JI1q[3Kxc3PvdIlkKGGwYXz5d4l{ MYqyNFcxQDl|Nx?=
human ReNcell VM cells MoS2VJJwdGmoZYLheIlwdiCjc4PhfS=> NY\vdHBxOyEQvF2= M4n1[|czKGh? MWXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKnTnPlcIwhXk1iY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBifCB|IIXNJIFnfGW{IEeyJIhzew>? NWXFbFdNOjB5MEi5N|c>
human SK-MEL-1 cell NGjQbYdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHe3bZRKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImsJGlEPTB;M{SuO|E1KM7:TR?= NX3HS3FuW0GQR1XS
human MOLT-4 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUHJcohq[mm2aX;uJI9nKGi3bXHuJG1QVFRvNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO2MlE1PjdizszN NUjLT5pyW0GQR1XS
human T47D cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWfJcohq[mm2aX;uJI9nKGi3bXHuJHQ1P0RiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|Nz6xOlQ4KM7:TR?= NWfININbW0GQR1XS
human SW1710 cell NHL6S45Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUXQTY9xUW6qaXLpeIlwdiCxZjDoeY1idiCVV{G3NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Py5|NkC4JO69VQ>? NYfkdpA4W0GQR1XS
human MKN7 cell MkjRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Ml;TTY5pcWKrdHnvckBw\iCqdX3hckBOU055IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{iuNFkxQSEQvF2= NV3YVGZKW0GQR1XS
human CAL-72 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVnJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC15MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO4MlEyPzhizszN Mkn6V2FPT0WU
human AGS cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlzXTY5pcWKrdHnvckBw\iCqdX3hckBCT1NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|OD6yNFM6KM7:TR?= NV\IXHF5W0GQR1XS
human BE-13 cell MkewS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NE\1SYpKdmirYnn0bY9vKG:oIHj1cYFvKEKHLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|gvPzd6MzFOwG0> MoXHV2FPT0WU
human Calu-6 cell NXnjd5NnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUPmSlA1UW6qaXLpeIlwdiCxZjDoeY1idiCFYXz1MVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{QS54OU[0JO69VQ>? MkC0V2FPT0WU
human CAL-27 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV7rUZdbUW6qaXLpeIlwdiCxZjDoeY1idiCFQVytNlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01PC5yMEW0JO69VQ>? M1ruSHNCVkeHUh?=
human BB49-HNC cell MkjtS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnvFTY5pcWKrdHnvckBw\iCqdX3hckBDSjR7LVjOR{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQ1NjB{N{ig{txO MYrTRW5ITVJ?

... Click to View More Cell Line Experimental Data

In vivo Administration of SB 216763 at 20 mg/kg significantly prevents lung inflammation and the subsequent fibrosis in bleomycin (BLM)-induced pulmonary inflammation and fibrosis model in mice by significantly blocking the production of inflammatory cytokines MCP-1 and TNF-α by macrophages, and significantly improves the survival of BLM-treated mice. SB 216763 treatment causes a significant reduction in BLM-induced alveolitis by inhibiting alveolar epithelial cell damage. [4]

Protocol

Kinase Assay:[1]
+ Expand

GSK-3 activity assay:

GSK-3 kinase activity is measured, in the presence of various concentrations of SB 216763, in a reaction mixture containing final concentrations of 1 nM human GSK-3α, 50 mM MOPS pH 7.0, 0.2 mM EDTA, 10 mM Mg-acetate, 7.5 mM β-mercaptoethanol, 5% (w/v) glycerol, 0.01% (w/v) Tween-20, 10% (v/v) DMSO, and 28 μM GS-2 peptide substrate. The GS-2 peptide sequence corresponds to a region of glycogen synthase that is phosphorylated by GSK-3. The assay is initiated by the addition of 0.34 μCi [33P]γ-ATP. The total ATP concentration is 10 μM. Following 30 minutes incubation at room temperature the assay is stopped by the addition of one third assay volume of 2.5% (v/v) H3PO4 containing 21 mM ATP. Samples are spotted onto P30 phosphocellulose mats and washed six times in 0.5% (v/v) H3PO4. The filter mats are sealed into sample bags containing Wallac betaplate scintillation fluid. 33P incorporation into the substrate peptide is determined by counting the mats in a Wallac microbeta scintillation counter.
Cell Research:[3]
+ Expand
  • Cell lines: BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC cells
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24, 48, and 72 hours
  • Method: Cells are exposed to various concentrations of SB 216763 for 24, 48 and 72 hours. Relative cell viability is measured using the MTS assay. Apoptotic cells are determined by staining with Hoechst.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: C57BL/6N mice with lung inflammation and fibrosis induced by bleomycin (BLM)
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 20 mg/kg
  • Administration: Intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 23 mg/mL (61.95 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+corn oil
For best results, use promptly after mixing.
4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.22
Formula

C19H12N2O2Cl2

CAS No. 280744-09-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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GSK-3 Signaling Pathway Map

GSK-3 Inhibitors with Unique Features

Related GSK-3 Products4

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID