SB216763

Catalog No.S1075

SB216763 Chemical Structure

Molecular Weight(MW): 371.22

SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.

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In DMSO USD 165 In stock
USD 147 In stock
USD 270 In stock
USD 870 In stock
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Cited by 15 Publications

4 Customer Reviews

  • Mol Cancer Ther 2014 13(1), 37-48. SB216763 purchased from Selleck.

  • Cancer Res, 2018, doi:10.1158/0008-5472. SB216763 purchased from Selleck.

  • Viability assays for five additional GSK3 inhibitors in SY5Y cells.

    Mol Cancer Ther, 2013, 13(2): 454-67 . SB216763 purchased from Selleck.

  • In the presence of SB216763 (2 nM), transwell migration and invasion assay was conducted to evaluate the cell metastasis after Serpine2 transfection. Data were from three independent experiments and were average ± S.E. values. **P < 0.01, compared to transfected vector cells; ##P < 0.01, compared to transfected Serpine2 cells.

    Am J Transl Res, 2016, 8(5):1985-97.. SB216763 purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.
Targets
GSK-3α [1]
(Cell-free assay)
GSK-3β [1]
(Cell-free assay)
34.3 nM ~34.3 nM
In vitro

SB 216763 displays similar potency for GSK-3β with 96% inhibition at 10 μM while exhibiting minimal activity against 24 other protein kinases including PKBα and PDK1 with IC50 of >10 μM. SB 216763 stimulates glycogen synthesis in human liver cells with EC50 of 3.6 μM, and induces dose-dependent transcription of a β-catenin-LEF/TCF regulated reporter gene in HEK293 cells with a maximum 2.5-fold induction at 5 μM. [1] SB 216763 protects the cerebellar granule neurones from apoptotic cell death induced by LY-294002 or potassium-deprivation in a concentration-dependent manner, with a maximal neuroprotection at 3 μM in contrast with the effect of lithium chloride at which 10 mM is required. SB 216763 at 3 μM also completely prevents death of chicken dorsal root ganglion sensory neurones induced by LY-294002 regardless of NGF. SB 216763 treatment at 5 μM markedly inhibits the GSK-3-dependent phosphorylation of neuronal-specific microtubule-associated protein tau in cerebellar granule neurones or recombinant tau in HEK293 cells, and induces increased levels of cytoplasmic β-catenin in both cells mimicking the effect of Wnt-mediated inhibition of GSK-3. [2] In pancreatic cancer cell lines including BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC, SB 216763 treatment at 25-50 μM reduces cell viability in a dose-dependent manner, and leads to significant increase in apoptosis by 50% at 72 hours due to the specific down regulation of GSK-3β, while has no effect in HMEC or WI38 cell lines. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells NV\LNlVoTnWwY4Tpc44h[XO|YYm= NWKxe3I5T2y7Y3;n[Y4he3mwdHjhd4Uh[WO2aY\peJkhd2ZiSFXLNlk{KGOnbHzzMEBqdmirYnn0bY9vKG:oIFfTT|Mu[mW2YTygSWM2OD1yLkKg{txO M4f4dFEzQTRzM{Ox
human HDLM-2 cell NGW0W2tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWTwfI1jUW6qaXLpeIlwdiCxZjDoeY1idiCKRFzNMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjJyOUi0JO69VQ>? M{PNNXNCVkeHUh?=
human NCI-H1770 cell NGPhXGJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIHpfnZKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVc4OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LjDJR|UxRTBwMkmzPVIh|ryP NFjZ[ldUSU6JRWK=
human HOP-62 cell M2C0b2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH;oZ5JKdmirYnn0bY9vKG:oIHj1cYFvKEiRUD22NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDV{NU[g{txO NHK4bW1USU6JRWK=
human JAR cell NWDLbGoxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYrJcohq[mm2aX;uJI9nKGi3bXHuJGpCWiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMkGwOFQh|ryP Mn:4V2FPT0WU
human K5 cell NU\sTIl7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIS0d5NKdmirYnn0bY9vKG:oIHj1cYFvKEt3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJktKEmFNUC9NU42OzZ7ODFOwG0> MVHTRW5ITVJ?
human MV-4-11 cell MnjuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX3Jcohq[mm2aX;uJI9nKGi3bXHuJG1XNTRvMUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlY6Pjh3IN88US=> Mn;IV2FPT0WU
human BT-549 cell MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEPkUZVKdmirYnn0bY9vKG:oIHj1cYFvKEKWLUW0PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvOjl|N{[g{txO MnXXV2FPT0WU
human LB2241-RCC cell MlHKS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUTJcohq[mm2aX;uJI9nKGi3bXHuJGxDOjJ2MT3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03NjR{OEe0JO69VQ>? MmrEV2FPT0WU
human GAMG cell MX\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWi5TY9VUW6qaXLpeIlwdiCxZjDoeY1idiCJQV3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok43PTRyOTFOwG0> NIDtT2pUSU6JRWK=
human HMV-II cell M2WyWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1HCRWlvcGmkaYTpc44hd2ZiaIXtZY4hUE2YLVnJJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{43PzZyNzFOwG0> NF3Be3lUSU6JRWK=
human RS4-11 cell NX;hNmcxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYDXc|FTUW6qaXLpeIlwdiCxZjDoeY1idiCUU{StNVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjJ2MUSxJO69VQ>? NFzsU3BUSU6JRWK=
human NCI-H1993 cell NHraZ4lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHrSO4pKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVk6OyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRThwM{[1N|Qh|ryP NWrUVJU6W0GQR1XS
human IST-SL1 cell NILxTItIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkPYTY5pcWKrdHnvckBw\iCqdX3hckBKW1RvU1yxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU4xOjB2IN88US=> MlzJV2FPT0WU
human SK-OV-3 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkXOTY5pcWKrdHnvckBw\iCqdX3hckBUUy2RVj2zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU4yODB{IN88US=> MXHTRW5ITVJ?
human ESS-1 cell NH3FRWpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{LIWmlvcGmkaYTpc44hd2ZiaIXtZY4hTVOVLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlU6QDd{IN88US=> M3KzPXNCVkeHUh?=
human G-361 cell MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHzF[HJKdmirYnn0bY9vKG:oIHj1cYFvKEdvM{[xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvOTd7ODFOwG0> M2f2PHNCVkeHUh?=
human ALL-PO cell Mor5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4C1XWlvcGmkaYTpc44hd2ZiaIXtZY4hSUyOLWDPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvOzR5NzFOwG0> NEPrXYhUSU6JRWK=
human NB5 cell M{fye2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVvJcohq[mm2aX;uJI9nKGi3bXHuJG5DPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFzLkKxOVch|ryP NIjkeplUSU6JRWK=
human DU-145 cell NGHMeVJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYXJcohq[mm2aX;uJI9nKGi3bXHuJGRWNTF2NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlY2OzVizszN NW\XT|ZTW0GQR1XS
human NMC-G1 cell NVqwZ5VKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVnQO|dIUW6qaXLpeIlwdiCxZjDoeY1idiCQTVOtS|Eh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOS55Mk[0JO69VQ>? M2HCbnNCVkeHUh?=
human RPMI-7951 cell NFrWepJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mlv6TY5pcWKrdHnvckBw\iCqdX3hckBTWE2LLUe5OVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigS5iSVO1NF0yOS55OE[0JO69VQ>? M1fxUHNCVkeHUh?=
human NCI-H2009 cell M1TsTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFvkcI5KdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlAxQSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTF|LkK0Nlch|ryP NFXDW49USU6JRWK=
human LoVo cell MkDkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mk\nTY5pcWKrdHnvckBw\iCqdX3hckBNd1[xIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUOuN|c4OSEQvF2= MoP3V2FPT0WU
human A2058 cell M2HzSmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M361d2lvcGmkaYTpc44hd2ZiaIXtZY4hSTJyNUigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{41PjlizszN NEW4OmtUSU6JRWK=
human D-566MG cell NVHFc4g2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVHPeo1EUW6qaXLpeIlwdiCxZjDoeY1idiCGLUW2Om1IKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTNwOUS4NUDPxE1? MVTTRW5ITVJ?
human QIMR-WIL cell MlvSS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1TZdWlvcGmkaYTpc44hd2ZiaIXtZY4hWUmPUj3XTWwh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPS54M{m0JO69VQ>? M2\w[HNCVkeHUh?=
human S-117 cell NXnVc|FbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYKyV3oxUW6qaXLpeIlwdiCxZjDoeY1idiCVLUGxO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE3NjRyMkKg{txO M{PYdHNCVkeHUh?=
human YH-13 cell MkLYS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NX\QcGJ7UW6qaXLpeIlwdiCxZjDoeY1idiC\SD2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE3NjZ6NkWg{txO NYXnTFZbW0GQR1XS
human IGROV-1 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NW\jcGRCUW6qaXLpeIlwdiCxZjDoeY1idiCLR2LPWk0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTdwMkCwNUDPxE1? NGnrfIhUSU6JRWK=
human BHT-101 cell MnzmS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFmybHpKdmirYnn0bY9vKG:oIHj1cYFvKEKKVD2xNFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPy54MEm5JO69VQ>? MnLoV2FPT0WU
human MKN45 cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2e0VmlvcGmkaYTpc44hd2ZiaIXtZY4hVUuQNEWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPE41OTJ6IN88US=> MVLTRW5ITVJ?
human A498 cell MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mmj2TY5pcWKrdHnvckBw\iCqdX3hckBCPDl6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUmuOFU1QSEQvF2= MUXTRW5ITVJ?
human U-266 cell NUnjT5pbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWrJSmtoUW6qaXLpeIlwdiCxZjDoeY1idiCXLUK2OkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIxNjh5OUeg{txO MUfTRW5ITVJ?
human BFTC-905 cell MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUnJcohq[mm2aX;uJI9nKGi3bXHuJGJHXENvOUC1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlEvOTh5OTFOwG0> M3XnR3NCVkeHUh?=
human BxPC-3 cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWPJcohq[mm2aX;uJI9nKGi3bXHuJGJ5WENvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKxMlM2PTRizszN MojHV2FPT0WU
human NCI-SNU-1 cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYfJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2VTmWtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIyNjV3NUig{txO MVTTRW5ITVJ?
human SF539 cell NXf6UZJWT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoXITY5pcWKrdHnvckBw\iCqdX3hckBUTjV|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKyMlAyQThizszN NX7ON3M3W0GQR1XS
human VA-ES-BJ cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUTJcohq[mm2aX;uJI9nKGi3bXHuJHZCNUWVLVLKJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlIvPTd4MzFOwG0> MVjTRW5ITVJ?
human HuO-3N1 cell NIjNb4hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnfRTY5pcWKrdHnvckBw\iCqdX3hckBJfU9vM16xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlIvPjF3MTFOwG0> M4\LeXNCVkeHUh?=
human EM-2 cell NX;YWGhzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1vD[WlvcGmkaYTpc44hd2ZiaIXtZY4hTU1vMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKyMlY3QDlizszN MXLTRW5ITVJ?
human T98G cell MlTuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXTXZnNkUW6qaXLpeIlwdiCxZjDoeY1idiCWOUjHJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlMvOzByOTFOwG0> NYL6W49SW0GQR1XS
human SW1783 cell M{PJNmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MX3Jcohq[mm2aX;uJI9nKGi3bXHuJHNYOTd6MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKzMlUzPDNizszN MnrTV2FPT0WU
human NKM-1 cell Mlj2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWXJcohq[mm2aX;uJI9nKGi3bXHuJG5MVS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkOuPFUyOiEQvF2= M3zWd3NCVkeHUh?=
human KOSC-2 cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVfmVWdIUW6qaXLpeIlwdiCxZjDoeY1idiCNT2PDMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPC5zMkmyJO69VQ>? MojpV2FPT0WU
human SW48 cell NHG4PGNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmHhTY5pcWKrdHnvckBw\iCqdX3hckBUXzR6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkSuOlA3PyEQvF2= MojzV2FPT0WU
human MIAPaCa2 cells NViyOY5YT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUCz[nY2PzJiaB?= M2Xrb2dzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1KSVCjQ3GyJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGOgZZN{[XluIFnDOVA:OjVizszN M1HCbFE6OzN6M{W1
human NCI-H28 cell M2fRdmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFrsWZhKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPS5{MUC0JO69VQ>? MWDTRW5ITVJ?
human 5637 cell NHjQfmtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{DicWlvcGmkaYTpc44hd2ZiaIXtZY4hPTZ|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK1MlgyOTFizszN NFrFR4dUSU6JRWK=
human NB69 cell M1TMeWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NG\Zc5RKdmirYnn0bY9vKG:oIHj1cYFvKE6ENkmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOk4yPzZ2IN88US=> M2DybHNCVkeHUh?=
human HT-29 cell NEjhfWRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MonnTY5pcWKrdHnvckBw\iCqdX3hckBJXC1{OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK3MlE4PTJizszN NG\LXlNUSU6JRWK=
human PFSK-1 cell NVTNcZZiT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGTKcJRKdmirYnn0bY9vKG:oIHj1cYFvKFCIU1utNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI4NjV7MzFOwG0> MVrTRW5ITVJ?
human UACC-257 cell NWfEfld5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVPJcohq[mm2aX;uJI9nKGi3bXHuJHVCS0NvMkW3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlcvPzR|IN88US=> Mkm0V2FPT0WU
human D-423MG cell M122Smdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVPJcohq[mm2aX;uJI9nKGi3bXHuJGQuPDJ|TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yO{46OTB4IN88US=> NGXjZo5USU6JRWK=
human NH-12 cell NHXXOXFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4XTUGlvcGmkaYTpc44hd2ZiaIXtZY4hVkhvMUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yPE41ODV7IN88US=> NYDr[5ZjW0GQR1XS
human DEL cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mk\PTY5pcWKrdHnvckBw\iCqdX3hckBFTUxiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{OT6wOFc1KM7:TR?= NECwTYhUSU6JRWK=
human LS-513 cell NHTYdYJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml3qTY5pcWKrdHnvckBw\iCqdX3hckBNWy13MUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zNE4yOzN2IN88US=> MlXNV2FPT0WU
human NBsusSR cell M4O3emdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4f1eGlvcGmkaYTpc44hd2ZiaIXtZY4hVkK|dYPTVkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMxNjV4N{ig{txO NUjzfHR3W0GQR1XS
human BV-173 cell NXjBUXZZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHLYXmRKdmirYnn0bY9vKG:oIHj1cYFvKEKYLUG3N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMxNjd4NEmg{txO M2LkS3NCVkeHUh?=
human A101D cell NXS0WpZkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2\4dGlvcGmkaYTpc44hd2ZiaIXtZY4hSTFyMVSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zNE46Pzh2IN88US=> MUjTRW5ITVJ?
human LU-134-A cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXPJcohq[mm2aX;uJI9nKGi3bXHuJGxWNTF|ND3BJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|EvODJ|ODFOwG0> NEjZNY1USU6JRWK=
human ES3 cell NVmxSoF5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHfw[4NKdmirYnn0bY9vKG:oIHj1cYFvKEWVMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOxMlM{PzZizszN NYHN[2dPW0GQR1XS
human NY cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4DMcmlvcGmkaYTpc44hd2ZiaIXtZY4hVlliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|Mj6yPVY2KM7:TR?= M4\NcXNCVkeHUh?=
human NCI-H1975 cell M2TiNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1z3cWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixPVc2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzJwM{W4NkDPxE1? Mmj6V2FPT0WU
human A704 cell Mmn5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkPUTY5pcWKrdHnvckBw\iCqdX3hckBCPzB2IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{SuNlA2QSEQvF2= M3LaXnNCVkeHUh?=
human SK-MEL-24 cell NYLK[Y93T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVPJcohq[mm2aX;uJI9nKGi3bXHuJHNMNU2HTD2yOEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVM1NjJ3MkOg{txO M1\hN3NCVkeHUh?=
human SW1088 cell M3LUVmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXrJcohq[mm2aX;uJI9nKGi3bXHuJHNYOTB6ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO0MlM1PTJizszN NUn1bXlDW0GQR1XS
human HepG2 cells NX7oeGJbTnWwY4Tpc44h[XO|YYm= MX[zOEDPxE1? MXGxNkBp MWLJcohq[mm2aX;uJI9nKEeVS{Pi[ZRiKGmwIHj1cYFvKEincFeyJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKHKjdHnvJI9nKHCqb4PwbI9zgWyjdHXkJGdUN0eVIHH0JFM1KHWPIHHmeIVzKDF{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= MWCyOVQ3PzF3MB?=
mouse 3T3L1 cells NGDVWmFHfW6ldHnvckBie3OjeR?= NGDL[4w{PCEQvF2= MonSNVIhcA>? MnXVTY5kemWjc3WgbY4h\2y3Y3;z[UBqdmOxcoDvdoF1cW:wIHnuJI1wfXOnIEPUN2wyKGOnbHzzJIF1KDN2IIXNJIFnfGW{IEGyJIhzeyCkeTDncJVkd3OnIHHzd4F6 MVGyOVQ3PzF3MB?=
human ST14A cells M1\Wd2Z2dmO2aX;uJIF{e2G7 MU[2JIg> MUnJcohq[mm2aX;uJI9nKEeVSz2zMYJmfGFvbXXkbYF1\WRiV370JJNq\26jbHnu[{BqdiCqdX3hckBUXDF2QTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBi[2O3bYXsZZRqd25ib3[gZoV1[S2lYYPlbY4h[XKxdX7kJI52[2yndYOgZYZ1\XJiNjDodpMh[nlibXnjdo9{[2:yaXOgZY5idHm|aYO= MWmyNFcxQDl|Nx?=
human ReNcell VM cells NFjBO2FRem:uaX\ldoF1cW:wIHHzd4F6 NVnNU2lmOyEQvF2= M{C1cFczKGh? MXXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKnTnPlcIwhXk1iY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBifCB|IIXNJIFnfGW{IEeyJIhzew>? M4r4VlIxPzB6OUO3
human SK-MEL-1 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4XaeWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTVXMMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTygTWM2OD1|ND63NVQh|ryP M1PIUXNCVkeHUh?=
human MOLT-4 cell NVWwO3RkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUHJcohq[mm2aX;uJI9nKGi3bXHuJG1QVFRvNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO2MlE1PjdizszN NYPKR|liW0GQR1XS
human T47D cell NXrOemtoT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2fEdmlvcGmkaYTpc44hd2ZiaIXtZY4hXDR5RDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO3MlE3PDdizszN MkX2V2FPT0WU
human SW1710 cell M1WxTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3XSVmlvcGmkaYTpc44hd2ZiaIXtZY4hW1dzN{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|cvOzZyODFOwG0> NFuyNJFUSU6JRWK=
human MKN7 cell NIK3UJNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXjJcohq[mm2aX;uJI9nKGi3bXHuJG1MVjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|OD6wPVA6KM7:TR?= M{jaeHNCVkeHUh?=
human CAL-72 cell MkLkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFvBcYxKdmirYnn0bY9vKG:oIHj1cYFvKEODTD23NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVM5NjFzN{ig{txO M3fhOXNCVkeHUh?=
human AGS cell Mlv1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUTJcohq[mm2aX;uJI9nKGi3bXHuJGFIWyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN6LkKwN|kh|ryP MkSwV2FPT0WU
human BE-13 cell NYjybo5JT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUDJcohq[mm2aX;uJI9nKGi3bXHuJGJGNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{iuO|c5OyEQvF2= MnjEV2FPT0WU
human Calu-6 cell MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NULHdItLUW6qaXLpeIlwdiCxZjDoeY1idiCFYXz1MVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{QS54OU[0JO69VQ>? Mlj5V2FPT0WU
human CAL-27 cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWnJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1{NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUS0MlAxPTRizszN MonpV2FPT0WU
human BB49-HNC cell M2fMVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWXJcohq[mm2aX;uJI9nKGi3bXHuJGJDPDlvSF7DJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OFQvODJ5ODFOwG0> MlXRV2FPT0WU

... Click to View More Cell Line Experimental Data

In vivo Administration of SB 216763 at 20 mg/kg significantly prevents lung inflammation and the subsequent fibrosis in bleomycin (BLM)-induced pulmonary inflammation and fibrosis model in mice by significantly blocking the production of inflammatory cytokines MCP-1 and TNF-α by macrophages, and significantly improves the survival of BLM-treated mice. SB 216763 treatment causes a significant reduction in BLM-induced alveolitis by inhibiting alveolar epithelial cell damage. [4]

Protocol

Kinase Assay:[1]
+ Expand

GSK-3 activity assay:

GSK-3 kinase activity is measured, in the presence of various concentrations of SB 216763, in a reaction mixture containing final concentrations of 1 nM human GSK-3α, 50 mM MOPS pH 7.0, 0.2 mM EDTA, 10 mM Mg-acetate, 7.5 mM β-mercaptoethanol, 5% (w/v) glycerol, 0.01% (w/v) Tween-20, 10% (v/v) DMSO, and 28 μM GS-2 peptide substrate. The GS-2 peptide sequence corresponds to a region of glycogen synthase that is phosphorylated by GSK-3. The assay is initiated by the addition of 0.34 μCi [33P]γ-ATP. The total ATP concentration is 10 μM. Following 30 minutes incubation at room temperature the assay is stopped by the addition of one third assay volume of 2.5% (v/v) H3PO4 containing 21 mM ATP. Samples are spotted onto P30 phosphocellulose mats and washed six times in 0.5% (v/v) H3PO4. The filter mats are sealed into sample bags containing Wallac betaplate scintillation fluid. 33P incorporation into the substrate peptide is determined by counting the mats in a Wallac microbeta scintillation counter.
Cell Research:[3]
+ Expand
  • Cell lines: BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC cells
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24, 48, and 72 hours
  • Method: Cells are exposed to various concentrations of SB 216763 for 24, 48 and 72 hours. Relative cell viability is measured using the MTS assay. Apoptotic cells are determined by staining with Hoechst.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: C57BL/6N mice with lung inflammation and fibrosis induced by bleomycin (BLM)
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 20 mg/kg
  • Administration: Intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 23 mg/mL (61.95 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+corn oil
For best results, use promptly after mixing.
4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.22
Formula

C19H12N2O2Cl2

CAS No. 280744-09-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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GSK-3 Signaling Pathway Map

GSK-3 Inhibitors with Unique Features

Related GSK-3 Products4

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID