SB216763

Catalog No.S1075

SB216763 Chemical Structure

Molecular Weight(MW): 371.22

SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.

Size Price Stock Quantity  
In DMSO USD 165 In stock
USD 147 In stock
USD 270 In stock
USD 870 In stock
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4 Customer Reviews

  • Cancer Res, 2018, doi:10.1158/0008-5472. SB216763 purchased from Selleck.

    Viability assays for five additional GSK3 inhibitors in SY5Y cells.

    Mol Cancer Ther, 2013, 13(2): 454-67 . SB216763 purchased from Selleck.

  • Mol Cancer Ther 2014 13(1), 37-48. SB216763 purchased from Selleck.

    In the presence of SB216763 (2 nM), transwell migration and invasion assay was conducted to evaluate the cell metastasis after Serpine2 transfection. Data were from three independent experiments and were average ± S.E. values. **P < 0.01, compared to transfected vector cells; ##P < 0.01, compared to transfected Serpine2 cells.

    Am J Transl Res, 2016, 8(5):1985-97.. SB216763 purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.
Targets
GSK-3α [1]
(Cell-free assay)
GSK-3β [1]
(Cell-free assay)
34.3 nM ~34.3 nM
In vitro

SB 216763 displays similar potency for GSK-3β with 96% inhibition at 10 μM while exhibiting minimal activity against 24 other protein kinases including PKBα and PDK1 with IC50 of >10 μM. SB 216763 stimulates glycogen synthesis in human liver cells with EC50 of 3.6 μM, and induces dose-dependent transcription of a β-catenin-LEF/TCF regulated reporter gene in HEK293 cells with a maximum 2.5-fold induction at 5 μM. [1] SB 216763 protects the cerebellar granule neurones from apoptotic cell death induced by LY-294002 or potassium-deprivation in a concentration-dependent manner, with a maximal neuroprotection at 3 μM in contrast with the effect of lithium chloride at which 10 mM is required. SB 216763 at 3 μM also completely prevents death of chicken dorsal root ganglion sensory neurones induced by LY-294002 regardless of NGF. SB 216763 treatment at 5 μM markedly inhibits the GSK-3-dependent phosphorylation of neuronal-specific microtubule-associated protein tau in cerebellar granule neurones or recombinant tau in HEK293 cells, and induces increased levels of cytoplasmic β-catenin in both cells mimicking the effect of Wnt-mediated inhibition of GSK-3. [2] In pancreatic cancer cell lines including BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC, SB 216763 treatment at 25-50 μM reduces cell viability in a dose-dependent manner, and leads to significant increase in apoptosis by 50% at 72 hours due to the specific down regulation of GSK-3β, while has no effect in HMEC or WI38 cell lines. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells MXvGeY5kfGmxbjDhd5NigQ>? NWrEdY1DT2y7Y3;n[Y4he3mwdHjhd4Uh[WO2aY\peJkhd2ZiSFXLNlk{KGOnbHzzMEBqdmirYnn0bY9vKG:oIFfTT|Mu[mW2YTygSWM2OD1yLkKg{txO M2Pub|EzQTRzM{Ox
human HDLM-2 cell M4P4[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVnzTGFIUW6qaXLpeIlwdiCxZjDoeY1idiCKRFzNMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjJyOUi0JO69VQ>? MWXTRW5ITVJ?
human NCI-H1770 cell NX3rSWhxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3XaUGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixO|cxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XlwIFnDOVA:OC5{OUO5NkDPxE1? M4DrN3NCVkeHUh?=
human HOP-62 cell Mn3VS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2fvU2lvcGmkaYTpc44hd2ZiaIXtZY4hUE:SLU[yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45PTJ3NjFOwG0> MYfTRW5ITVJ?
human JAR cell NV\MXYVZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MX3Jcohq[mm2aX;uJI9nKGi3bXHuJGpCWiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMkGwOFQh|ryP NHjue3VUSU6JRWK=
human K5 cell NWn4VI9ST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYLRXphwUW6qaXLpeIlwdiCxZjDoeY1idiCNNTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6NCCLQ{WwQVEvPTN4OUig{txO NYq2OFRuW0GQR1XS
human MV-4-11 cell NHT0PHRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWLJcohq[mm2aX;uJI9nKGi3bXHuJG1XNTRvMUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlY6Pjh3IN88US=> M4\nVXNCVkeHUh?=
human BT-549 cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX2zbYhqUW6qaXLpeIlwdiCxZjDoeY1idiCEVD21OFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjJ7M{e2JO69VQ>? M{jlVnNCVkeHUh?=
human LB2241-RCC cell NUDzfmF2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MX3Jcohq[mm2aX;uJI9nKGi3bXHuJGxDOjJ2MT3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03NjR{OEe0JO69VQ>? MnfvV2FPT0WU
human GAMG cell NXLZTYVyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MofaTY5pcWKrdHnvckBw\iCqdX3hckBISU2JIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj62OVQxQSEQvF2= MVTTRW5ITVJ?
human HMV-II cell NYX5eod7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoHITY5pcWKrdHnvckBw\iCqdX3hckBJVVZvSVmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlY4PjB5IN88US=> M4fSS3NCVkeHUh?=
human RS4-11 cell MnX1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEfPPYtKdmirYnn0bY9vKG:oIHj1cYFvKFKVND2xNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvOjRzNEGg{txO M4nFcXNCVkeHUh?=
human NCI-H1993 cell MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{jjWWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixPVk{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QC5|NkWzOEDPxE1? NWnPcVRvW0GQR1XS
human IST-SL1 cell NFTsWVJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVLJcohq[mm2aX;uJI9nKGi3bXHuJGlUXC2VTEGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlAzODRizszN NG[3TpZUSU6JRWK=
human SK-OV-3 cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXmxPVJqUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3PWk0{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QS5zMECyJO69VQ>? MWLTRW5ITVJ?
human ESS-1 cell NVr1c4ZnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEfxTYVKdmirYnn0bY9vKG:oIHj1cYFvKEWVUz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU42QTh5MjFOwG0> M1\yV3NCVkeHUh?=
human G-361 cell MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4G4UmlvcGmkaYTpc44hd2ZiaIXtZY4hTy1|NkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE4yPzl6IN88US=> NGPVVIpUSU6JRWK=
human ALL-PO cell MmjXS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUHJcohq[mm2aX;uJI9nKGi3bXHuJGFNVC2STzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlM1PzdizszN M{HhZnNCVkeHUh?=
human NB5 cell NEXs[5pIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUfJcohq[mm2aX;uJI9nKGi3bXHuJG5DPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFzLkKxOVch|ryP M2q4S3NCVkeHUh?=
human DU-145 cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2nPPWlvcGmkaYTpc44hd2ZiaIXtZY4hTFVvMUS1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvPjV|NTFOwG0> MmLSV2FPT0WU
human NMC-G1 cell NGXMS49Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVTJcohq[mm2aX;uJI9nKGi3bXHuJG5OSy2JMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlczPjRizszN MkSwV2FPT0WU
human RPMI-7951 cell Mk[3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4DoWWlvcGmkaYTpc44hd2ZiaIXtZY4hWlCPST23PVUyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XlwIFnDOVA:OTFwN{i2OEDPxE1? NHL0V|lUSU6JRWK=
human NCI-H2009 cell Mo\4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2TK[2lvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFA6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTNwMkSyO{DPxE1? MVHTRW5ITVJ?
human LoVo cell NUPmdJd7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWnkfIo3UW6qaXLpeIlwdiCxZjDoeY1idiCOb2\vJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvOzd5MTFOwG0> M1O0TXNCVkeHUh?=
human A2058 cell NVXX[mc4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M37pNmlvcGmkaYTpc44hd2ZiaIXtZY4hSTJyNUigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{41PjlizszN NEGzVXlUSU6JRWK=
human D-566MG cell MknlS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M37HOWlvcGmkaYTpc44hd2ZiaIXtZY4hTC13Nk\NS{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{Njl2OEGg{txO M3TJSXNCVkeHUh?=
human QIMR-WIL cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MX7Jcohq[mm2aX;uJI9nKGi3bXHuJHFKVVJvV1nMJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvPjN7NDFOwG0> MWrTRW5ITVJ?
human S-117 cell M3jO[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUDlSZJkUW6qaXLpeIlwdiCxZjDoeY1idiCVLUGxO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE3NjRyMkKg{txO NV\JeZZTW0GQR1XS
human YH-13 cell NYjy[nZzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4PLS2lvcGmkaYTpc44hd2ZiaIXtZY4hYUhvMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOk43QDZ3IN88US=> NVHofJJzW0GQR1XS
human IGROV-1 cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NICwcItKdmirYnn0bY9vKG:oIHj1cYFvKEmJUl;WMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPy5{MECxJO69VQ>? M{LxNHNCVkeHUh?=
human BHT-101 cell NFjOe|JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV3Jcohq[mm2aX;uJI9nKGi3bXHuJGJJXC1zMEGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xO{43ODl7IN88US=> MUPTRW5ITVJ?
human MKN45 cell Mnu0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWPVPItwUW6qaXLpeIlwdiCxZjDoeY1idiCPS160OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE5NjRzMkig{txO NWXyUoNuW0GQR1XS
human A498 cell NILuXmxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MW\Jcohq[mm2aX;uJI9nKGi3bXHuJGE1QThiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zOT60OVQ6KM7:TR?= NYjqS|dJW0GQR1XS
human U-266 cell NHLxPGtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M164RWlvcGmkaYTpc44hd2ZiaIXtZY4hXS1{Nk[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNE45Pzl5IN88US=> MXPTRW5ITVJ?
human BFTC-905 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXXJcohq[mm2aX;uJI9nKGi3bXHuJGJHXENvOUC1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlEvOTh5OTFOwG0> MnLLV2FPT0WU
human BxPC-3 cell M1KxVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3;ReGlvcGmkaYTpc44hd2ZiaIXtZY4hSniSQz2zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlEvOzV3NDFOwG0> NVyxOHhbW0GQR1XS
human NCI-SNU-1 cell Ml3PS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWLicnVJUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtV25WNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{MT61OVU5KM7:TR?= MUnTRW5ITVJ?
human SF539 cell NUT6TGZ{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYPJcohq[mm2aX;uJI9nKGi3bXHuJHNHPTN7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkKuNFE6QCEQvF2= MWDTRW5ITVJ?
human VA-ES-BJ cell NXzSeJEzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3HRVWlvcGmkaYTpc44hd2ZiaIXtZY4hXkFvRWOtRmoh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOi53N{[zJO69VQ>? NIK3SYhUSU6JRWK=
human HuO-3N1 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkC5TY5pcWKrdHnvckBw\iCqdX3hckBJfU9vM16xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlIvPjF3MTFOwG0> MoX2V2FPT0WU
human EM-2 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NE\ueWxKdmirYnn0bY9vKG:oIHj1cYFvKEWPLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNk43Pjh7IN88US=> NGHIcm9USU6JRWK=
human T98G cell NGDQZpVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnPOTY5pcWKrdHnvckBw\iCqdX3hckBVQTiJIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkOuN|AxQSEQvF2= NGPXdXVUSU6JRWK=
human SW1783 cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MknUTY5pcWKrdHnvckBw\iCqdX3hckBUXzF5OEOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yN{42OjR|IN88US=> NFyzPW5USU6JRWK=
human NKM-1 cell NH\PVVBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NE\heJJKdmirYnn0bY9vKG:oIHj1cYFvKE6NTT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlMvQDVzMjFOwG0> NHHrS5NUSU6JRWK=
human KOSC-2 cell NXX0UJkxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV7KdZZMUW6qaXLpeIlwdiCxZjDoeY1idiCNT2PDMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPC5zMkmyJO69VQ>? M3XvfnNCVkeHUh?=
human SW48 cell M1S2Vmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2DwNGlvcGmkaYTpc44hd2ZiaIXtZY4hW1d2ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK0MlYxPjdizszN NVvlO4VLW0GQR1XS
human MIAPaCa2 cells M1X2NWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEjJS5U4OiCq MkHtS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUWlCWGGFYUKgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF0zPSEQvF2= NUTlSmZoOTl|M{izOVU>
human NCI-H28 cell MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIrUOIhKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPS5{MUC0JO69VQ>? Mmm5V2FPT0WU
human 5637 cell MlHMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1jHN2lvcGmkaYTpc44hd2ZiaIXtZY4hPTZ|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK1MlgyOTFizszN MkDrV2FPT0WU
human NB69 cell NXHudpZKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUfJcohq[mm2aX;uJI9nKGi3bXHuJG5DPjliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{Nj6xO|Y1KM7:TR?= M4fSS3NCVkeHUh?=
human HT-29 cell NIW4[4dIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NH3EU|BKdmirYnn0bY9vKG:oIHj1cYFvKEiWLUK5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlcvOTd3MjFOwG0> NVfIN3ZOW0GQR1XS
human PFSK-1 cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVfJcohq[mm2aX;uJI9nKGi3bXHuJHBHW0tvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK3MlU6OyEQvF2= MYnTRW5ITVJ?
human UACC-257 cell NYTje2xXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIXZOVlKdmirYnn0bY9vKG:oIHj1cYFvKFWDQ1OtNlU4KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjdwN{SzJO69VQ>? MUPTRW5ITVJ?
human D-423MG cell M{nWS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWPJcohq[mm2aX;uJI9nKGi3bXHuJGQuPDJ|TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yO{46OTB4IN88US=> MljDV2FPT0WU
human NH-12 cell MlvmS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHfvcXBKdmirYnn0bY9vKG:oIHj1cYFvKE6KLUGyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlgvPDB3OTFOwG0> M12yN3NCVkeHUh?=
human DEL cell NHjLbJlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHy3cYtKdmirYnn0bY9vKG:oIHj1cYFvKESHTDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK5MlA1PzRizszN MYnTRW5ITVJ?
human LS-513 cell M4C5fGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmG1TY5pcWKrdHnvckBw\iCqdX3hckBNWy13MUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zNE4yOzN2IN88US=> MoPNV2FPT0WU
human NBsusSR cell NX7CNXEzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NU[zW3JsUW6qaXLpeIlwdiCxZjDoeY1idiCQQoP1d3NTKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzBwNU[3PEDPxE1? NUHaNWdrW0GQR1XS
human BV-173 cell NYrUd2g4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NETqUm9KdmirYnn0bY9vKG:oIHj1cYFvKEKYLUG3N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMxNjd4NEmg{txO NV7wZlMyW0GQR1XS
human A101D cell M1nmOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV\pephHUW6qaXLpeIlwdiCxZjDoeY1idiCDMUCxSEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMxNjl5OESg{txO MXrTRW5ITVJ?
human LU-134-A cell NF3iOo9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkC0TY5pcWKrdHnvckBw\iCqdX3hckBNXS1zM{StRUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMyNjB{M{ig{txO M2XCcnNCVkeHUh?=
human ES3 cell M{jVe2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF7G[mZKdmirYnn0bY9vKG:oIHj1cYFvKEWVMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOxMlM{PzZizszN NH7LdWFUSU6JRWK=
human NY cell NHnoVnpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVHJcohq[mm2aX;uJI9nKGi3bXHuJG5[KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzJwMkm2OUDPxE1? MmnlV2FPT0WU
human NCI-H1975 cell NVraW3k4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2rjc2lvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixPVc2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzJwM{W4NkDPxE1? MXPTRW5ITVJ?
human A704 cell NHq0bG1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NH;K[pBKdmirYnn0bY9vKG:oIHj1cYFvKEF5MESgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zOE4zODV7IN88US=> NX7jSoFMW0GQR1XS
human SK-MEL-24 cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NG\PXnZKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0zPCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN2LkK1NlMh|ryP MVvTRW5ITVJ?
human SW1088 cell NWnJXmhET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3Lv[GlvcGmkaYTpc44hd2ZiaIXtZY4hW1dzMEi4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|QvOzR3MjFOwG0> NGWxelRUSU6JRWK=
human HepG2 cells NFfTb|NHfW6ldHnvckBie3OjeR?= M4HJTFM1KM7:TR?= NIC0OIkyOiCq MYfJcohq[mm2aX;uJI9nKEeVS{Pi[ZRiKGmwIHj1cYFvKEincFeyJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKHKjdHnvJI9nKHCqb4PwbI9zgWyjdHXkJGdUN0eVIHH0JFM1KHWPIHHmeIVzKDF{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= NFHJdHUzPTR4N{G1NC=>
mouse 3T3L1 cells MX;GeY5kfGmxbjDhd5NigQ>? M{L3PFM1KM7:TR?= NED2ZWcyOiCq MnHwTY5kemWjc3WgbY4h\2y3Y3;z[UBqdmOxcoDvdoF1cW:wIHnuJI1wfXOnIEPUN2wyKGOnbHzzJIF1KDN2IIXNJIFnfGW{IEGyJIhzeyCkeTDncJVkd3OnIHHzd4F6 NHSyclIzPTR4N{G1NC=>
human ST14A cells NH31Z3hHfW6ldHnvckBie3OjeR?= MWi2JIg> MknXTY5pcWKrdHnvckBw\iCJU1utN{1j\XSjLX3l[IlifGWmIGfueEB{cWewYXzpcochcW5iaIXtZY4hW1RzNFGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4h[WOldX31cIF1cW:wIH;mJIJmfGFvY3Hz[YlvKGG{b4Xu[EBvfWOuZYXzJIFnfGW{IE[gbJJ{KGK7IH3pZ5Jwe2OxcHnjJIFv[Wy7c3nz M2rKdVIxPzB6OUO3
human ReNcell VM cells MV;Qdo9tcW[ncnH0bY9vKGG|c3H5 M{i4TlMh|ryP NXLQeHNCPzJiaB?= NF;uUG9CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGLlUoNmdGxiVl2gZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJI9nKGOnbHygdJJwdGmoZYLheIlwdiCjdDCzJJVOKGGodHXyJFczKGi{cx?= M2TiV|IxPzB6OUO3
human SK-MEL-1 cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1H3W2lvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTVXMMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTygTWM2OD1|ND63NVQh|ryP MVHTRW5ITVJ?
human MOLT-4 cell MkP0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnXGTY5pcWKrdHnvckBw\iCqdX3hckBOV0yWLUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zOk4yPDZ5IN88US=> MXPTRW5ITVJ?
human T47D cell MYfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnjoTY5pcWKrdHnvckBw\iCqdX3hckBVPDeGIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{euNVY1PyEQvF2= NHXxXpFUSU6JRWK=
human SW1710 cell M3K2TWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHTlTZpKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUexNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVM4NjN4MEig{txO NX3OdY5wW0GQR1XS
human MKN7 cell NW\OeXFvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NF;Tb|VKdmirYnn0bY9vKG:oIHj1cYFvKE2NTkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPE4xQTB7IN88US=> MUPTRW5ITVJ?
human CAL-72 cell M2ToUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkiwTY5pcWKrdHnvckBw\iCqdX3hckBESUxvN{KgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPE4yOTd6IN88US=> NUjZSJpzW0GQR1XS
human AGS cell NUHJSpNrT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4LYcGlvcGmkaYTpc44hd2ZiaIXtZY4hSUeVIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{iuNlA{QSEQvF2= M{LldXNCVkeHUh?=
human BE-13 cell M1HEVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NELJd3dKdmirYnn0bY9vKG:oIHj1cYFvKEKHLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|gvPzd6MzFOwG0> MUHTRW5ITVJ?
human Calu-6 cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3XHVmlvcGmkaYTpc44hd2ZiaIXtZY4hS2GudT22JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|kvPjl4NDFOwG0> M{XQVXNCVkeHUh?=
human CAL-27 cell NFjMS4tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NV;nfXBbUW6qaXLpeIlwdiCxZjDoeY1idiCFQVytNlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01PC5yMEW0JO69VQ>? MmjVV2FPT0WU
human BB49-HNC cell NX7RcWxIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1\LOGlvcGmkaYTpc44hd2ZiaIXtZY4hSkJ2OT3IUmMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01PC5yMke4JO69VQ>? M1LLSXNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo Administration of SB 216763 at 20 mg/kg significantly prevents lung inflammation and the subsequent fibrosis in bleomycin (BLM)-induced pulmonary inflammation and fibrosis model in mice by significantly blocking the production of inflammatory cytokines MCP-1 and TNF-α by macrophages, and significantly improves the survival of BLM-treated mice. SB 216763 treatment causes a significant reduction in BLM-induced alveolitis by inhibiting alveolar epithelial cell damage. [4]

Protocol

Kinase Assay:[1]
+ Expand

GSK-3 activity assay:

GSK-3 kinase activity is measured, in the presence of various concentrations of SB 216763, in a reaction mixture containing final concentrations of 1 nM human GSK-3α, 50 mM MOPS pH 7.0, 0.2 mM EDTA, 10 mM Mg-acetate, 7.5 mM β-mercaptoethanol, 5% (w/v) glycerol, 0.01% (w/v) Tween-20, 10% (v/v) DMSO, and 28 μM GS-2 peptide substrate. The GS-2 peptide sequence corresponds to a region of glycogen synthase that is phosphorylated by GSK-3. The assay is initiated by the addition of 0.34 μCi [33P]γ-ATP. The total ATP concentration is 10 μM. Following 30 minutes incubation at room temperature the assay is stopped by the addition of one third assay volume of 2.5% (v/v) H3PO4 containing 21 mM ATP. Samples are spotted onto P30 phosphocellulose mats and washed six times in 0.5% (v/v) H3PO4. The filter mats are sealed into sample bags containing Wallac betaplate scintillation fluid. 33P incorporation into the substrate peptide is determined by counting the mats in a Wallac microbeta scintillation counter.
Cell Research:[3]
+ Expand
  • Cell lines: BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC cells
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24, 48, and 72 hours
  • Method: Cells are exposed to various concentrations of SB 216763 for 24, 48 and 72 hours. Relative cell viability is measured using the MTS assay. Apoptotic cells are determined by staining with Hoechst.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: C57BL/6N mice with lung inflammation and fibrosis induced by bleomycin (BLM)
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 20 mg/kg
  • Administration: Intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 23 mg/mL (61.95 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+corn oil
For best results, use promptly after mixing.
4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.22
Formula

C19H12N2O2Cl2

CAS No. 280744-09-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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GSK-3 Signaling Pathway Map

GSK-3 Inhibitors with Unique Features

Related GSK-3 Products4

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID