SB216763

Catalog No.S1075

SB216763 Chemical Structure

Molecular Weight(MW): 371.22

SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.

Size Price Stock Quantity  
In DMSO USD 165 In stock
USD 147 In stock
USD 270 In stock
USD 870 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

4 Customer Reviews

  • Cancer Res, 2018, doi:10.1158/0008-5472. SB216763 purchased from Selleck.

    Viability assays for five additional GSK3 inhibitors in SY5Y cells.

    Mol Cancer Ther, 2013, 13(2): 454-67 . SB216763 purchased from Selleck.

  • Mol Cancer Ther 2014 13(1), 37-48. SB216763 purchased from Selleck.

    In the presence of SB216763 (2 nM), transwell migration and invasion assay was conducted to evaluate the cell metastasis after Serpine2 transfection. Data were from three independent experiments and were average ± S.E. values. **P < 0.01, compared to transfected vector cells; ##P < 0.01, compared to transfected Serpine2 cells.

    Am J Transl Res, 2016, 8(5):1985-97.. SB216763 purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.
Targets
GSK-3α [1]
(Cell-free assay)
GSK-3β [1]
(Cell-free assay)
34.3 nM ~34.3 nM
In vitro

SB 216763 displays similar potency for GSK-3β with 96% inhibition at 10 μM while exhibiting minimal activity against 24 other protein kinases including PKBα and PDK1 with IC50 of >10 μM. SB 216763 stimulates glycogen synthesis in human liver cells with EC50 of 3.6 μM, and induces dose-dependent transcription of a β-catenin-LEF/TCF regulated reporter gene in HEK293 cells with a maximum 2.5-fold induction at 5 μM. [1] SB 216763 protects the cerebellar granule neurones from apoptotic cell death induced by LY-294002 or potassium-deprivation in a concentration-dependent manner, with a maximal neuroprotection at 3 μM in contrast with the effect of lithium chloride at which 10 mM is required. SB 216763 at 3 μM also completely prevents death of chicken dorsal root ganglion sensory neurones induced by LY-294002 regardless of NGF. SB 216763 treatment at 5 μM markedly inhibits the GSK-3-dependent phosphorylation of neuronal-specific microtubule-associated protein tau in cerebellar granule neurones or recombinant tau in HEK293 cells, and induces increased levels of cytoplasmic β-catenin in both cells mimicking the effect of Wnt-mediated inhibition of GSK-3. [2] In pancreatic cancer cell lines including BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC, SB 216763 treatment at 25-50 μM reduces cell viability in a dose-dependent manner, and leads to significant increase in apoptosis by 50% at 72 hours due to the specific down regulation of GSK-3β, while has no effect in HMEC or WI38 cell lines. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells M2HpeGZ2dmO2aX;uJIF{e2G7 NUD2WI5HT2y7Y3;n[Y4he3mwdHjhd4Uh[WO2aY\peJkhd2ZiSFXLNlk{KGOnbHzzMEBqdmirYnn0bY9vKG:oIFfTT|Mu[mW2YTygSWM2OD1yLkKg{txO MoDnNVI6PDF|M{G=
human HDLM-2 cell NVHufJVXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3nVRmlvcGmkaYTpc44hd2ZiaIXtZY4hUESOTT2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE4zODl6NDFOwG0> M2r6VnNCVkeHUh?=
human NCI-H1770 cell NFjB[VRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEnNSXNKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVc4OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LjDJR|UxRTBwMkmzPVIh|ryP MmC1V2FPT0WU
human HOP-62 cell M4D3[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkTMTY5pcWKrdHnvckBw\iCqdX3hckBJV1BvNkKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg2OjV4IN88US=> NXzQS|h2W0GQR1XS
human JAR cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWj0PXV3UW6qaXLpeIlwdiCxZjDoeY1idiCMQWKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlIyODR2IN88US=> NGDjOldUSU6JRWK=
human K5 cell Ml[xS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mor2TY5pcWKrdHnvckBw\iCqdX3hckBMPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5MEBKSzVyPUGuOVM3QThizszN M1fSTnNCVkeHUh?=
human MV-4-11 cell NV64fnczT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXPFTldHUW6qaXLpeIlwdiCxZjDoeY1idiCPVj20MVEyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi54OU[4OUDPxE1? MXHTRW5ITVJ?
human BT-549 cell NXq1R45OT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mm\nTY5pcWKrdHnvckBw\iCqdX3hckBDXC13NEmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlI6Ozd4IN88US=> MWjTRW5ITVJ?
human LB2241-RCC cell MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUDGZplpUW6qaXLpeIlwdiCxZjDoeY1idiCOQkKyOFEuWkOFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj60Nlg4PCEQvF2= M2D6fnNCVkeHUh?=
human GAMG cell Mmq5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{TpNGlvcGmkaYTpc44hd2ZiaIXtZY4hT0GPRzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPU[uOlU1ODlizszN NWHGOJdFW0GQR1XS
human HMV-II cell NGjZbYhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M17EWGlvcGmkaYTpc44hd2ZiaIXtZY4hUE2YLVnJJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{43PzZyNzFOwG0> M3njW3NCVkeHUh?=
human RS4-11 cell NEDDdJRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4rTXmlvcGmkaYTpc44hd2ZiaIXtZY4hWlN2LUGxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE4zPDF2MTFOwG0> M33RNXNCVkeHUh?=
human NCI-H1993 cell NEfSTIxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXf0Z5JCUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE6QTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16LkO2OVM1KM7:TR?= NH32bXdUSU6JRWK=
human IST-SL1 cell NFXVZ4NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWriTm04UW6qaXLpeIlwdiCxZjDoeY1idiCLU2StV2wyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QS5yMkC0JO69VQ>? M3PZeHNCVkeHUh?=
human SK-OV-3 cell NFnEPG1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3HrPWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvT2[tN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvOTByMjFOwG0> NV3zUmd4W0GQR1XS
human ESS-1 cell NX3i[3hvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXfJcohq[mm2aX;uJI9nKGi3bXHuJGVUWy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OT61PVg4OiEQvF2= MWTTRW5ITVJ?
human G-361 cell M2W3PGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnHrTY5pcWKrdHnvckBw\iCqdX3hckBINTN4MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlE4QThizszN M3jtS3NCVkeHUh?=
human ALL-PO cell MlnUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlvvTY5pcWKrdHnvckBw\iCqdX3hckBCVExvUF:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE4{PDd5IN88US=> NULD[npXW0GQR1XS
human NB5 cell M4fnT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlPFTY5pcWKrdHnvckBw\iCqdX3hckBPSjViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT6yNVU4KM7:TR?= NYm2O4NyW0GQR1XS
human DU-145 cell MlmxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYTJcohq[mm2aX;uJI9nKGi3bXHuJGRWNTF2NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlY2OzVizszN NXnvWZcxW0GQR1XS
human NMC-G1 cell NYj3[nVXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NU\ue2t3UW6qaXLpeIlwdiCxZjDoeY1idiCQTVOtS|Eh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOS55Mk[0JO69VQ>? MUTTRW5ITVJ?
human RPMI-7951 cell M2jlVmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mn7DTY5pcWKrdHnvckBw\iCqdX3hckBTWE2LLUe5OVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigS5iSVO1NF0yOS55OE[0JO69VQ>? MYPTRW5ITVJ?
human NCI-H2009 cell MlvsS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVjvSlFQUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFIxODliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMz6yOFI4KM7:TR?= NXu1bmxXW0GQR1XS
human LoVo cell NXfO[XBIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MX\Jcohq[mm2aX;uJI9nKGi3bXHuJGxwXm9iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMz6zO|cyKM7:TR?= NWOxSZVKW0GQR1XS
human A2058 cell M{fqNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlTHTY5pcWKrdHnvckBw\iCqdX3hckBCOjB3ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlQ3QSEQvF2= MYrTRW5ITVJ?
human D-566MG cell Mn30S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYDJcohq[mm2aX;uJI9nKGi3bXHuJGQuPTZ4TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{46PDhzIN88US=> MWPTRW5ITVJ?
human QIMR-WIL cell M4f6cWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnrvTY5pcWKrdHnvckBw\iCqdX3hckBSUU2ULWfJUEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE2NjZ|OUSg{txO NXHyZ2o5W0GQR1XS
human S-117 cell NGPSNoRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXTJcohq[mm2aX;uJI9nKGi3bXHuJHMuOTF5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MU[uOFAzOiEQvF2= M2e0V3NCVkeHUh?=
human YH-13 cell M1T4RWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYLJcohq[mm2aX;uJI9nKGi3bXHuJHlJNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MU[uOlg3PSEQvF2= MmPmV2FPT0WU
human IGROV-1 cell NV3rWVYyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4r0[mlvcGmkaYTpc44hd2ZiaIXtZY4hUUeUT2[tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE4NjJyMEGg{txO MmH0V2FPT0WU
human BHT-101 cell NVHBO|FlT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIHBWVRKdmirYnn0bY9vKG:oIHj1cYFvKEKKVD2xNFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPy54MEm5JO69VQ>? NUPPVIpvW0GQR1XS
human MKN45 cell MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2rreGlvcGmkaYTpc44hd2ZiaIXtZY4hVUuQNEWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPE41OTJ6IN88US=> MV7TRW5ITVJ?
human A498 cell Ml3sS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1rUXWlvcGmkaYTpc44hd2ZiaIXtZY4hSTR7ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG5MlQ2PDlizszN NUHpVplkW0GQR1XS
human U-266 cell MYDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYrXbGdZUW6qaXLpeIlwdiCxZjDoeY1idiCXLUK2OkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIxNjh5OUeg{txO M1rFcnNCVkeHUh?=
human BFTC-905 cell M1zIOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mnf6TY5pcWKrdHnvckBw\iCqdX3hckBDTlSFLUmwOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIyNjF6N{mg{txO NFjVW2xUSU6JRWK=
human BxPC-3 cell M{TZbWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mlz1TY5pcWKrdHnvckBw\iCqdX3hckBDgFCFLUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNU4{PTV2IN88US=> NG\aelNUSU6JRWK=
human NCI-SNU-1 cell MkPhS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3XQcWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLWPOWU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjFwNUW1PEDPxE1? NVTRTYl4W0GQR1XS
human SF539 cell NYPlT|NPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4\JV2lvcGmkaYTpc44hd2ZiaIXtZY4hW0Z3M{mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNk4xOTl6IN88US=> MV\TRW5ITVJ?
human VA-ES-BJ cell M{\USWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEHybZNKdmirYnn0bY9vKG:oIHj1cYFvKF[DLVXTMWJLKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjJwNUe2N{DPxE1? NXy4[HNsW0GQR1XS
human HuO-3N1 cell M{TXO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3PxbmlvcGmkaYTpc44hd2ZiaIXtZY4hUHWRLUPONUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIzNjZzNUGg{txO NEe5fWNUSU6JRWK=
human EM-2 cell NUPySXdNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NH\kSmNKdmirYnn0bY9vKG:oIHj1cYFvKEWPLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNk43Pjh7IN88US=> NITybGNUSU6JRWK=
human T98G cell MVvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2e0UWlvcGmkaYTpc44hd2ZiaIXtZY4hXDl6RzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKzMlMxODlizszN NF;HWFBUSU6JRWK=
human SW1783 cell MmmwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVi1cHR3UW6qaXLpeIlwdiCxZjDoeY1idiCVV{G3PFMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOy53MkSzJO69VQ>? NIjkXHdUSU6JRWK=
human NKM-1 cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGnZZodKdmirYnn0bY9vKG:oIHj1cYFvKE6NTT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlMvQDVzMjFOwG0> M1y0[XNCVkeHUh?=
human KOSC-2 cell NVLVXGpiT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXzJcohq[mm2aX;uJI9nKGi3bXHuJGtQW0NvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK0MlEzQTJizszN NYPpcmpCW0GQR1XS
human SW48 cell M2PnWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4nvUmlvcGmkaYTpc44hd2ZiaIXtZY4hW1d2ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK0MlYxPjdizszN MVXTRW5ITVJ?
human MIAPaCa2 cells MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX;BcFFQPzJiaB?= MYDHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNTWFR[UOjMjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSVIHHzd4F6NCCLQ{WwQVI2KM7:TR?= NVrNbphZOTl|M{izOVU>
human NCI-H28 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MY\Jcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOU4zOTB2IN88US=> NF7seYxUSU6JRWK=
human 5637 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2XObGlvcGmkaYTpc44hd2ZiaIXtZY4hPTZ|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK1MlgyOTFizszN NUTxdI9jW0GQR1XS
human NB69 cell NI\BU4JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWi3bo9xUW6qaXLpeIlwdiCxZjDoeY1idiCQQk[5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlYvOTd4NDFOwG0> M4PGc3NCVkeHUh?=
human HT-29 cell MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWPPdHFDUW6qaXLpeIlwdiCxZjDoeY1idiCKVD2yPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI4NjF5NUKg{txO NHnoNY5USU6JRWK=
human PFSK-1 cell NV32Z2FLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmTNTY5pcWKrdHnvckBw\iCqdX3hckBRTlONLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yO{42QTNizszN NF3LNo5USU6JRWK=
human UACC-257 cell M1vMV2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWjJcohq[mm2aX;uJI9nKGi3bXHuJHVCS0NvMkW3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlcvPzR|IN88US=> NVvmV241W0GQR1XS
human D-423MG cell NEnKOnhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGrBPWhKdmirYnn0bY9vKG:oIHj1cYFvKERvNEKzUWch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPy57MUC2JO69VQ>? MWHTRW5ITVJ?
human NH-12 cell NVLtNWpsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHzyTndKdmirYnn0bY9vKG:oIHj1cYFvKE6KLUGyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlgvPDB3OTFOwG0> M4HnbXNCVkeHUh?=
human DEL cell M1vlcGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYXJcohq[mm2aX;uJI9nKGi3bXHuJGRGVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJ7LkC0O|Qh|ryP M4fGWHNCVkeHUh?=
human LS-513 cell NYXmWnlRT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NInr[VRKdmirYnn0bY9vKG:oIHj1cYFvKEyVLUWxN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMxNjF|M{Sg{txO M1z0VHNCVkeHUh?=
human NBsusSR cell NUe1cJBOT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFvlOI1KdmirYnn0bY9vKG:oIHj1cYFvKE6Ec4XzV3Ih[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{OC53Nke4JO69VQ>? NWTO[ZAxW0GQR1XS
human BV-173 cell NFXGUmdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVLJcohq[mm2aX;uJI9nKGi3bXHuJGJXNTF5MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOwMlc3PDlizszN M{HQbnNCVkeHUh?=
human A101D cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{HQZWlvcGmkaYTpc44hd2ZiaIXtZY4hSTFyMVSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zNE46Pzh2IN88US=> M2\wXHNCVkeHUh?=
human LU-134-A cell MnrqS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWOweXdGUW6qaXLpeIlwdiCxZjDoeY1idiCOVT2xN|QuSSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTNzLkCyN|gh|ryP NX71OoJoW0GQR1XS
human ES3 cell M3LOT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYLk[GFnUW6qaXLpeIlwdiCxZjDoeY1idiCHU{OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zNU4{Ozd4IN88US=> NGq4R5pUSU6JRWK=
human NY cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFuxV2NKdmirYnn0bY9vKG:oIHj1cYFvKE6\IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{KuNlk3PSEQvF2= NWPVXnJDW0GQR1XS
human NCI-H1975 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{LpdGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixPVc2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzJwM{W4NkDPxE1? NHHHXpNUSU6JRWK=
human A704 cell NIX1fndIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3G2c2lvcGmkaYTpc44hd2ZiaIXtZY4hSTdyNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO0MlIxPTlizszN MVPTRW5ITVJ?
human SK-MEL-24 cell MlTxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3nRZWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTVXMMVI1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzRwMkWyN{DPxE1? NG\C[21USU6JRWK=
human SW1088 cell MkXpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWrJcohq[mm2aX;uJI9nKGi3bXHuJHNYOTB6ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO0MlM1PTJizszN NYfBXmhVW0GQR1XS
human HepG2 cells Ml\PSpVv[3Srb36gZZN{[Xl? NH;tfIo{PCEQvF2= NWDQbVhrOTJiaB?= NF3rTopKdmirYnn0bY9vKG:oIFfTT|Nj\XSjIHnuJIh2dWGwIFjldGczKGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIILheIlwKG:oIIDoc5NxcG:{eXzheIVlKEeVL1fTJIF1KDN2IIXNJIFnfGW{IEGyJIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> MWGyOVQ3PzF3MB?=
mouse 3T3L1 cells M1TjSmZ2dmO2aX;uJIF{e2G7 NFjabVY{PCEQvF2= MXmxNkBp NH7oS4hKdmO{ZXHz[UBqdiCpbIXjc5NmKGmwY3;ydI9z[XSrb36gbY4hdW:3c3WgN3Q{VDFiY3XscJMh[XRiM{SgeW0h[W[2ZYKgNVIhcHK|IHL5JIdtfWOxc3WgZZN{[Xl? NG\LUpEzPTR4N{G1NC=>
human ST14A cells MmXQSpVv[3Srb36gZZN{[Xl? MYC2JIg> M33rWGlvcGmkaYTpc44hd2ZiR2PLMVMu[mW2YT3t[YRq[XSnZDDXcpQhe2mpbnHsbY5oKGmwIHj1cYFvKFOWMUTBJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGGlY4XteYxifGmxbjDv[kBj\XSjLXPhd4VqdiCjcn;1coQhdnWlbHX1d{Bi\nSncjC2JIhzeyCkeTDtbYNzd3Olb4DpZ{BidmGueYPpdy=> NUG3Z2JHOjB5MEi5N|c>
human ReNcell VM cells MVvQdo9tcW[ncnH0bY9vKGG|c3H5 MlPkN{DPxE1? MYm3NkBp MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKnTnPlcIwhXk1iY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBifCB|IIXNJIFnfGW{IEeyJIhzew>? M{LJN|IxPzB6OUO3
human SK-MEL-1 cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFv2UZRKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImsJGlEPTB;M{SuO|E1KM7:TR?= M{\WZ3NCVkeHUh?=
human MOLT-4 cell NVPNTZNyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXTJcohq[mm2aX;uJI9nKGi3bXHuJG1QVFRvNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO2MlE1PjdizszN NV20S4xTW0GQR1XS
human T47D cell MmjQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEjTV|dKdmirYnn0bY9vKG:oIHj1cYFvKFR2N1SgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zO{4yPjR5IN88US=> MmfaV2FPT0WU
human SW1710 cell NH;CZWNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXXjU4YzUW6qaXLpeIlwdiCxZjDoeY1idiCVV{G3NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Py5|NkC4JO69VQ>? MYPTRW5ITVJ?
human MKN7 cell M4OyNGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnvlTY5pcWKrdHnvckBw\iCqdX3hckBOU055IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{iuNFkxQSEQvF2= NYfrNVFvW0GQR1XS
human CAL-72 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXfn[nJsUW6qaXLpeIlwdiCxZjDoeY1idiCFQVytO|Ih[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{QC5zMUe4JO69VQ>? M135THNCVkeHUh?=
human AGS cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVfJcohq[mm2aX;uJI9nKGi3bXHuJGFIWyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN6LkKwN|kh|ryP NX23Rm5FW0GQR1XS
human BE-13 cell NHrOVoJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn\tTY5pcWKrdHnvckBw\iCqdX3hckBDTS1zMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO4Mlc4QDNizszN NEHleYJUSU6JRWK=
human Calu-6 cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlrPTY5pcWKrdHnvckBw\iCqdX3hckBE[Wy3LU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPU43QTZ2IN88US=> NIG2UmZUSU6JRWK=
human CAL-27 cell NEnsb5VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3G1W2lvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUK3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OFQvODB3NDFOwG0> MnTuV2FPT0WU
human BB49-HNC cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUe1V5c6UW6qaXLpeIlwdiCxZjDoeY1idiCEQkS5MWhPSyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTR2LkCyO|gh|ryP NIHNNpVUSU6JRWK=

... Click to View More Cell Line Experimental Data

In vivo Administration of SB 216763 at 20 mg/kg significantly prevents lung inflammation and the subsequent fibrosis in bleomycin (BLM)-induced pulmonary inflammation and fibrosis model in mice by significantly blocking the production of inflammatory cytokines MCP-1 and TNF-α by macrophages, and significantly improves the survival of BLM-treated mice. SB 216763 treatment causes a significant reduction in BLM-induced alveolitis by inhibiting alveolar epithelial cell damage. [4]

Protocol

Kinase Assay:[1]
+ Expand

GSK-3 activity assay:

GSK-3 kinase activity is measured, in the presence of various concentrations of SB 216763, in a reaction mixture containing final concentrations of 1 nM human GSK-3α, 50 mM MOPS pH 7.0, 0.2 mM EDTA, 10 mM Mg-acetate, 7.5 mM β-mercaptoethanol, 5% (w/v) glycerol, 0.01% (w/v) Tween-20, 10% (v/v) DMSO, and 28 μM GS-2 peptide substrate. The GS-2 peptide sequence corresponds to a region of glycogen synthase that is phosphorylated by GSK-3. The assay is initiated by the addition of 0.34 μCi [33P]γ-ATP. The total ATP concentration is 10 μM. Following 30 minutes incubation at room temperature the assay is stopped by the addition of one third assay volume of 2.5% (v/v) H3PO4 containing 21 mM ATP. Samples are spotted onto P30 phosphocellulose mats and washed six times in 0.5% (v/v) H3PO4. The filter mats are sealed into sample bags containing Wallac betaplate scintillation fluid. 33P incorporation into the substrate peptide is determined by counting the mats in a Wallac microbeta scintillation counter.
Cell Research:[3]
+ Expand
  • Cell lines: BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC cells
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24, 48, and 72 hours
  • Method: Cells are exposed to various concentrations of SB 216763 for 24, 48 and 72 hours. Relative cell viability is measured using the MTS assay. Apoptotic cells are determined by staining with Hoechst.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: C57BL/6N mice with lung inflammation and fibrosis induced by bleomycin (BLM)
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 20 mg/kg
  • Administration: Intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 23 mg/mL (61.95 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+corn oil
For best results, use promptly after mixing.
4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.22
Formula

C19H12N2O2Cl2

CAS No. 280744-09-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

GSK-3 Signaling Pathway Map

GSK-3 Inhibitors with Unique Features

Related GSK-3 Products4

Tags: buy SB216763 | SB216763 supplier | purchase SB216763 | SB216763 cost | SB216763 manufacturer | order SB216763 | SB216763 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID