SB216763

Catalog No.S1075

SB216763 Chemical Structure

Molecular Weight(MW): 371.22

SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.

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In DMSO USD 165 In stock
USD 147 In stock
USD 270 In stock
USD 870 In stock
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Cited by 11 Publications

4 Customer Reviews

  • Cancer Res, 2018, doi:10.1158/0008-5472. SB216763 purchased from Selleck.

    Viability assays for five additional GSK3 inhibitors in SY5Y cells.

    Mol Cancer Ther, 2013, 13(2): 454-67 . SB216763 purchased from Selleck.

  • Mol Cancer Ther 2014 13(1), 37-48. SB216763 purchased from Selleck.

    In the presence of SB216763 (2 nM), transwell migration and invasion assay was conducted to evaluate the cell metastasis after Serpine2 transfection. Data were from three independent experiments and were average ± S.E. values. **P < 0.01, compared to transfected vector cells; ##P < 0.01, compared to transfected Serpine2 cells.

    Am J Transl Res, 2016, 8(5):1985-97.. SB216763 purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.
Targets
GSK-3α [1]
(Cell-free assay)
GSK-3β [1]
(Cell-free assay)
34.3 nM ~34.3 nM
In vitro

SB 216763 displays similar potency for GSK-3β with 96% inhibition at 10 μM while exhibiting minimal activity against 24 other protein kinases including PKBα and PDK1 with IC50 of >10 μM. SB 216763 stimulates glycogen synthesis in human liver cells with EC50 of 3.6 μM, and induces dose-dependent transcription of a β-catenin-LEF/TCF regulated reporter gene in HEK293 cells with a maximum 2.5-fold induction at 5 μM. [1] SB 216763 protects the cerebellar granule neurones from apoptotic cell death induced by LY-294002 or potassium-deprivation in a concentration-dependent manner, with a maximal neuroprotection at 3 μM in contrast with the effect of lithium chloride at which 10 mM is required. SB 216763 at 3 μM also completely prevents death of chicken dorsal root ganglion sensory neurones induced by LY-294002 regardless of NGF. SB 216763 treatment at 5 μM markedly inhibits the GSK-3-dependent phosphorylation of neuronal-specific microtubule-associated protein tau in cerebellar granule neurones or recombinant tau in HEK293 cells, and induces increased levels of cytoplasmic β-catenin in both cells mimicking the effect of Wnt-mediated inhibition of GSK-3. [2] In pancreatic cancer cell lines including BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC, SB 216763 treatment at 25-50 μM reduces cell viability in a dose-dependent manner, and leads to significant increase in apoptosis by 50% at 72 hours due to the specific down regulation of GSK-3β, while has no effect in HMEC or WI38 cell lines. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells MX;GeY5kfGmxbjDhd5NigQ>? NXSzd|ZFT2y7Y3;n[Y4he3mwdHjhd4Uh[WO2aY\peJkhd2ZiSFXLNlk{KGOnbHzzMEBqdmirYnn0bY9vKG:oIFfTT|Mu[mW2YTygSWM2OD1yLkKg{txO NUnj[nBQOTJ7NEGzN|E>
human HDLM-2 cell NGXFVFZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoHMTY5pcWKrdHnvckBw\iCqdX3hckBJTEyPLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlIxQTh2IN88US=> NVzyRVZPW0GQR1XS
human NCI-H1770 cell NGTNcllIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mk\sTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEG3O|Ah[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigS5iSVO1NF0xNjJ7M{myJO69VQ>? M{f4PHNCVkeHUh?=
human HOP-62 cell NV32[m41T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NF;jTFBKdmirYnn0bY9vKG:oIHj1cYFvKEiRUD22NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDV{NU[g{txO M2H1b3NCVkeHUh?=
human JAR cell NIrBeGJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEHQSmZKdmirYnn0bY9vKG:oIHj1cYFvKEqDUjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNlExPDRizszN NX[yUGZYW0GQR1XS
human K5 cell NF;NR|NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4CxWGlvcGmkaYTpc44hd2ZiaIXtZY4hUzViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUwhUUN3ME2xMlU{Pjl6IN88US=> NUnIZlc5W0GQR1XS
human MV-4-11 cell NVLIOnA4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkjHTY5pcWKrdHnvckBw\iCqdX3hckBOXi12LUGxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk43QTZ6NTFOwG0> M3f3cXNCVkeHUh?=
human BT-549 cell NWK4No4{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NE\qSmtKdmirYnn0bY9vKG:oIHj1cYFvKEKWLUW0PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvOjl|N{[g{txO MXzTRW5ITVJ?
human LB2241-RCC cell M{PoS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2PsUGlvcGmkaYTpc44hd2ZiaIXtZY4hVEJ{MkSxMXJESyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTZwNEK4O|Qh|ryP MluwV2FPT0WU
human GAMG cell MmTJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYjJcohq[mm2aX;uJI9nKGi3bXHuJGdCVUdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14Lk[1OFA6KM7:TR?= MUHTRW5ITVJ?
human HMV-II cell NFXEe5dIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnnsTY5pcWKrdHnvckBw\iCqdX3hckBJVVZvSVmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlY4PjB5IN88US=> NEHxd3pUSU6JRWK=
human RS4-11 cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVjpT3ZnUW6qaXLpeIlwdiCxZjDoeY1idiCUU{StNVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjJ2MUSxJO69VQ>? NXTWUWhbW0GQR1XS
human NCI-H1993 cell NVPKNZp6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYexXVViUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE6QTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16LkO2OVM1KM7:TR?= Mlv1V2FPT0WU
human IST-SL1 cell M1Hi[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVfJcohq[mm2aX;uJI9nKGi3bXHuJGlUXC2VTEGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlAzODRizszN NX3PV5hYW0GQR1XS
human SK-OV-3 cell M2TyT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MW\Jcohq[mm2aX;uJI9nKGi3bXHuJHNMNU:YLUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlExODJizszN M2LwUXNCVkeHUh?=
human ESS-1 cell NXW5O2R{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVqyXZVDUW6qaXLpeIlwdiCxZjDoeY1idiCHU2OtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvPTl6N{Kg{txO MkLUV2FPT0WU
human G-361 cell NXX3NpdsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGrPbHBKdmirYnn0bY9vKG:oIHj1cYFvKEdvM{[xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvOTd7ODFOwG0> NG\iNnpUSU6JRWK=
human ALL-PO cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVHWTY5NUW6qaXLpeIlwdiCxZjDoeY1idiCDTFytVG8h[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOC5|NEe3JO69VQ>? NUPVS45JW0GQR1XS
human NB5 cell NFu3dWdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml3MTY5pcWKrdHnvckBw\iCqdX3hckBPSjViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT6yNVU4KM7:TR?= Mlz1V2FPT0WU
human DU-145 cell NGPTeVNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{\GbGlvcGmkaYTpc44hd2ZiaIXtZY4hTFVvMUS1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvPjV|NTFOwG0> MULTRW5ITVJ?
human NMC-G1 cell M16zWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFzYO3NKdmirYnn0bY9vKG:oIHj1cYFvKE6PQz3HNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjd{NkSg{txO MWLTRW5ITVJ?
human RPMI-7951 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGHzXIZKdmirYnn0bY9vKG:oIHj1cYFvKFKSTVmtO|k2OSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LjDJR|UxRTFzLke4OlQh|ryP NUTpfVdPW0GQR1XS
human NCI-H2009 cell MX3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYrJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkCwPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{NjJ2Mkeg{txO NWnjW|l4W0GQR1XS
human LoVo cell MlHhS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV7Jcohq[mm2aX;uJI9nKGi3bXHuJGxwXm9iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMz6zO|cyKM7:TR?= NWP6[4Z6W0GQR1XS
human A2058 cell M4jiUmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUjJcohq[mm2aX;uJI9nKGi3bXHuJGEzODV6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUOuOFY6KM7:TR?= NITKR2FUSU6JRWK=
human D-566MG cell Mm\kS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUPJcohq[mm2aX;uJI9nKGi3bXHuJGQuPTZ4TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{46PDhzIN88US=> MWLTRW5ITVJ?
human QIMR-WIL cell NH\oTVVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXfPWpFvUW6qaXLpeIlwdiCxZjDoeY1idiCTSV3SMXdKVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTF3Lk[zPVQh|ryP M4P3NnNCVkeHUh?=
human S-117 cell MkP5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4\GO2lvcGmkaYTpc44hd2ZiaIXtZY4hWy1zMUegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOk41ODJ{IN88US=> M3vLTXNCVkeHUh?=
human YH-13 cell M4L5[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mn30TY5pcWKrdHnvckBw\iCqdX3hckB[UC1zMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG2MlY5PjVizszN NHTiNHVUSU6JRWK=
human IGROV-1 cell MYDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{nVNWlvcGmkaYTpc44hd2ZiaIXtZY4hUUeUT2[tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE4NjJyMEGg{txO NEL3ZXJUSU6JRWK=
human BHT-101 cell MnrES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4P0PWlvcGmkaYTpc44hd2ZiaIXtZY4hSkiWLUGwNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE4NjZyOUmg{txO NILTT49USU6JRWK=
human MKN45 cell NGW4PGpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1jHcWlvcGmkaYTpc44hd2ZiaIXtZY4hVUuQNEWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPE41OTJ6IN88US=> NUj0[GpCW0GQR1XS
human A498 cell Mmr5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX3Jcohq[mm2aX;uJI9nKGi3bXHuJGE1QThiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zOT60OVQ6KM7:TR?= M1q5OHNCVkeHUh?=
human U-266 cell NWfKd2t6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVvmZ|NsUW6qaXLpeIlwdiCxZjDoeY1idiCXLUK2OkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIxNjh5OUeg{txO NGLkR2lUSU6JRWK=
human BFTC-905 cell M1fRUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmHHTY5pcWKrdHnvckBw\iCqdX3hckBDTlSFLUmwOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIyNjF6N{mg{txO M2XUeHNCVkeHUh?=
human BxPC-3 cell NFTobZpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUDJcohq[mm2aX;uJI9nKGi3bXHuJGJ5WENvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKxMlM2PTRizszN NW\JSHl3W0GQR1XS
human NCI-SNU-1 cell M2P5[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXvMSlYyUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtV25WNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{MT61OVU5KM7:TR?= MmT4V2FPT0WU
human SF539 cell MkfaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MojzTY5pcWKrdHnvckBw\iCqdX3hckBUTjV|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKyMlAyQThizszN Ml\5V2FPT0WU
human VA-ES-BJ cell Mnj5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGLqRWJKdmirYnn0bY9vKG:oIHj1cYFvKF[DLVXTMWJLKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjJwNUe2N{DPxE1? NGr0TnRUSU6JRWK=
human HuO-3N1 cell NEHDfVRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmPjTY5pcWKrdHnvckBw\iCqdX3hckBJfU9vM16xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlIvPjF3MTFOwG0> MWrTRW5ITVJ?
human EM-2 cell M3OwVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1rOU2lvcGmkaYTpc44hd2ZiaIXtZY4hTU1vMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKyMlY3QDlizszN MV;TRW5ITVJ?
human T98G cell M2OwdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHzBUI9KdmirYnn0bY9vKG:oIHj1cYFvKFR7OFegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yN{4{ODB7IN88US=> NXzhVYprW0GQR1XS
human SW1783 cell NH;RU3RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M37QRmlvcGmkaYTpc44hd2ZiaIXtZY4hW1dzN{izJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlMvPTJ2MzFOwG0> MUTTRW5ITVJ?
human NKM-1 cell MkSwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWXJcohq[mm2aX;uJI9nKGi3bXHuJG5MVS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkOuPFUyOiEQvF2= M3PwcXNCVkeHUh?=
human KOSC-2 cell NHPSVnZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmfDTY5pcWKrdHnvckBw\iCqdX3hckBMV1OFLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOE4yOjl{IN88US=> NUTPTmlYW0GQR1XS
human SW48 cell MVvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NF\lXpFKdmirYnn0bY9vKG:oIHj1cYFvKFOZNEigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOE43ODZ5IN88US=> M1LrS3NCVkeHUh?=
human MIAPaCa2 cells NIXZWpFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXS3NkBp MV3Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNTWFR[UOjMjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSVIHHzd4F6NCCLQ{WwQVI2KM7:TR?= MVGxPVM{QDN3NR?=
human NCI-H28 cell Mn;tS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NELCXJBKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPS5{MUC0JO69VQ>? MoDQV2FPT0WU
human 5637 cell Mn3BS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYOzR5ExUW6qaXLpeIlwdiCxZjDoeY1idiB3NkO3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlUvQDFzMTFOwG0> MVHTRW5ITVJ?
human NB69 cell NHrwUVVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYr4cpV2UW6qaXLpeIlwdiCxZjDoeY1idiCQQk[5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlYvOTd4NDFOwG0> NXj6dFc2W0GQR1XS
human HT-29 cell M1Xrfmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1TIfGlvcGmkaYTpc44hd2ZiaIXtZY4hUFRvMkmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yO{4yPzV{IN88US=> NH\0eodUSU6JRWK=
human PFSK-1 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M13iTmlvcGmkaYTpc44hd2ZiaIXtZY4hWE[VSz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlcvPTl|IN88US=> M4HmbnNCVkeHUh?=
human UACC-257 cell NXLKNWN6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4XSOGlvcGmkaYTpc44hd2ZiaIXtZY4hXUGFQz2yOVch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPy55NEOg{txO NI\WfXlUSU6JRWK=
human D-423MG cell M2r5emdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWPObmU6UW6qaXLpeIlwdiCxZjDoeY1idiCGLUSyN21IKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjdwOUGwOkDPxE1? MknNV2FPT0WU
human NH-12 cell M1H3bmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlPVTY5pcWKrdHnvckBw\iCqdX3hckBPUC1zMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK4MlQxPTlizszN NY\3ephKW0GQR1XS
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human LS-513 cell NFW3ZoJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHjiXpJKdmirYnn0bY9vKG:oIHj1cYFvKEyVLUWxN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMxNjF|M{Sg{txO MkDoV2FPT0WU
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human BV-173 cell M4m0b2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3mx[WlvcGmkaYTpc44hd2ZiaIXtZY4hSlZvMUezJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|AvPzZ2OTFOwG0> NF\zWJBUSU6JRWK=
human A101D cell NUfI[29rT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWDJcohq[mm2aX;uJI9nKGi3bXHuJGEyODGGIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{CuPVc5PCEQvF2= MoPxV2FPT0WU
human LU-134-A cell NHXScmdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkPHTY5pcWKrdHnvckBw\iCqdX3hckBNXS1zM{StRUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMyNjB{M{ig{txO NVzD[VZCW0GQR1XS
human ES3 cell NHX5dG9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX7Jcohq[mm2aX;uJI9nKGi3bXHuJGVUOyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTNzLkOzO|Yh|ryP NWPhXHhZW0GQR1XS
human NY cell NF3PUmJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF3XOYpKdmirYnn0bY9vKG:oIHj1cYFvKE6\IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{KuNlk3PSEQvF2= MV;TRW5ITVJ?
human NCI-H1975 cell M3[0XGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoLoTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEG5O|Uh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Oi5|NUiyJO69VQ>? NWj2T3k6W0GQR1XS
human A704 cell NFLsW2pIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWHJcohq[mm2aX;uJI9nKGi3bXHuJGE4ODRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|ND6yNFU6KM7:TR?= MmHrV2FPT0WU
human SK-MEL-24 cell M{nIVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEK4OXFKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0zPCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN2LkK1NlMh|ryP NXfSfnZRW0GQR1XS
human SW1088 cell MoLZS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnfsTY5pcWKrdHnvckBw\iCqdX3hckBUXzFyOEigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zOE4{PDV{IN88US=> M{fydnNCVkeHUh?=
human HepG2 cells MXHGeY5kfGmxbjDhd5NigQ>? MUOzOEDPxE1? Mke4NVIhcA>? MYrJcohq[mm2aX;uJI9nKEeVS{Pi[ZRiKGmwIHj1cYFvKEincFeyJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKHKjdHnvJI9nKHCqb4PwbI9zgWyjdHXkJGdUN0eVIHH0JFM1KHWPIHHmeIVzKDF{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= MnfKNlU1PjdzNUC=
mouse 3T3L1 cells NYLndIRsTnWwY4Tpc44h[XO|YYm= NGrDPHE{PCEQvF2= MkXZNVIhcA>? NV;VUpg1UW6lcnXhd4UhcW5iZ3z1Z49{\SCrbnPvdpBwemG2aX;uJIlvKG2xdYPlJFNVO0xzIHPlcIx{KGG2IEO0JJVOKGGodHXyJFEzKGi{czDifUBodHWlb4PlJIF{e2G7 NGHET48zPTR4N{G1NC=>
human ST14A cells NFHvNHlHfW6ldHnvckBie3OjeR?= NYHqTHZnPiCq NF3keGxKdmirYnn0bY9vKG:oIFfTT{0{NWKndHGtcYVlcWG2ZXSgW451KHOrZ37hcIlv\yCrbjDoeY1idiCVVEG0RUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCjY3P1cZVt[XSrb36gc4Yh[mW2YT3jZZNmcW5iYYLveY5lKG63Y3zleZMh[W[2ZYKgOkBpenNiYomgcYlkem:|Y3;wbYMh[W6jbInzbZM> MXWyNFcxQDl|Nx?=
human ReNcell VM cells MWLQdo9tcW[ncnH0bY9vKGG|c3H5 NGLQe2U{KM7:TR?= MlPhO|IhcA>? MWnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKnTnPlcIwhXk1iY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBifCB|IIXNJIFnfGW{IEeyJIhzew>? NXT5eW1iOjB5MEi5N|c>
human SK-MEL-1 cell M1rPNGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1LBTWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTVXMMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTygTWM2OD1|ND63NVQh|ryP M{S0RnNCVkeHUh?=
human MOLT-4 cell NH;zO4FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIf2[lZKdmirYnn0bY9vKG:oIHj1cYFvKE2RTGStOEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVM3NjF2Nkeg{txO NHXoUGdUSU6JRWK=
human T47D cell NIrC[JNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFe2emhKdmirYnn0bY9vKG:oIHj1cYFvKFR2N1SgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zO{4yPjR5IN88US=> MX7TRW5ITVJ?
human SW1710 cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnjqTY5pcWKrdHnvckBw\iCqdX3hckBUXzF5MUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zO{4{PjB6IN88US=> M13XcnNCVkeHUh?=
human MKN7 cell NGPzTotIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYThTnFlUW6qaXLpeIlwdiCxZjDoeY1idiCPS163JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|gvODlyOTFOwG0> NV3peGx1W0GQR1XS
human CAL-72 cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmnyTY5pcWKrdHnvckBw\iCqdX3hckBESUxvN{KgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPE4yOTd6IN88US=> NILUUlNUSU6JRWK=
human AGS cell M3rNUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXzJcohq[mm2aX;uJI9nKGi3bXHuJGFIWyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN6LkKwN|kh|ryP M3LPcXNCVkeHUh?=
human BE-13 cell M2HobWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4\5[2lvcGmkaYTpc44hd2ZiaIXtZY4hSkVvMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPE44Pzh|IN88US=> NXvZS2gyW0GQR1XS
human Calu-6 cell M{THT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4DYWWlvcGmkaYTpc44hd2ZiaIXtZY4hS2GudT22JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|kvPjl4NDFOwG0> MYHTRW5ITVJ?
human CAL-27 cell MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWrJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1{NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUS0MlAxPTRizszN MnT4V2FPT0WU
human BB49-HNC cell NV7RWFdZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NW\YU5k2UW6qaXLpeIlwdiCxZjDoeY1idiCEQkS5MWhPSyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTR2LkCyO|gh|ryP NYS4VWVFW0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo Administration of SB 216763 at 20 mg/kg significantly prevents lung inflammation and the subsequent fibrosis in bleomycin (BLM)-induced pulmonary inflammation and fibrosis model in mice by significantly blocking the production of inflammatory cytokines MCP-1 and TNF-α by macrophages, and significantly improves the survival of BLM-treated mice. SB 216763 treatment causes a significant reduction in BLM-induced alveolitis by inhibiting alveolar epithelial cell damage. [4]

Protocol

Kinase Assay:[1]
+ Expand

GSK-3 activity assay:

GSK-3 kinase activity is measured, in the presence of various concentrations of SB 216763, in a reaction mixture containing final concentrations of 1 nM human GSK-3α, 50 mM MOPS pH 7.0, 0.2 mM EDTA, 10 mM Mg-acetate, 7.5 mM β-mercaptoethanol, 5% (w/v) glycerol, 0.01% (w/v) Tween-20, 10% (v/v) DMSO, and 28 μM GS-2 peptide substrate. The GS-2 peptide sequence corresponds to a region of glycogen synthase that is phosphorylated by GSK-3. The assay is initiated by the addition of 0.34 μCi [33P]γ-ATP. The total ATP concentration is 10 μM. Following 30 minutes incubation at room temperature the assay is stopped by the addition of one third assay volume of 2.5% (v/v) H3PO4 containing 21 mM ATP. Samples are spotted onto P30 phosphocellulose mats and washed six times in 0.5% (v/v) H3PO4. The filter mats are sealed into sample bags containing Wallac betaplate scintillation fluid. 33P incorporation into the substrate peptide is determined by counting the mats in a Wallac microbeta scintillation counter.
Cell Research:[3]
+ Expand
  • Cell lines: BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC cells
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24, 48, and 72 hours
  • Method: Cells are exposed to various concentrations of SB 216763 for 24, 48 and 72 hours. Relative cell viability is measured using the MTS assay. Apoptotic cells are determined by staining with Hoechst.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: C57BL/6N mice with lung inflammation and fibrosis induced by bleomycin (BLM)
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 20 mg/kg
  • Administration: Intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 23 mg/mL (61.95 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+corn oil
For best results, use promptly after mixing.
4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.22
Formula

C19H12N2O2Cl2

CAS No. 280744-09-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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GSK-3 Signaling Pathway Map

GSK-3 Inhibitors with Unique Features

Related GSK-3 Products4

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID