SB216763

Catalog No.S1075

SB216763 Chemical Structure

Molecular Weight(MW): 371.22

SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.

Size Price Stock Quantity  
In DMSO USD 165 In stock
USD 147 In stock
USD 270 In stock
USD 870 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

4 Customer Reviews

  • Cancer Res, 2018, doi:10.1158/0008-5472. SB216763 purchased from Selleck.

    Viability assays for five additional GSK3 inhibitors in SY5Y cells.

    Mol Cancer Ther, 2013, 13(2): 454-67 . SB216763 purchased from Selleck.

  • Mol Cancer Ther 2014 13(1), 37-48. SB216763 purchased from Selleck.

    In the presence of SB216763 (2 nM), transwell migration and invasion assay was conducted to evaluate the cell metastasis after Serpine2 transfection. Data were from three independent experiments and were average ± S.E. values. **P < 0.01, compared to transfected vector cells; ##P < 0.01, compared to transfected Serpine2 cells.

    Am J Transl Res, 2016, 8(5):1985-97.. SB216763 purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.
Targets
GSK-3α [1]
(Cell-free assay)
GSK-3β [1]
(Cell-free assay)
34.3 nM ~34.3 nM
In vitro

SB 216763 displays similar potency for GSK-3β with 96% inhibition at 10 μM while exhibiting minimal activity against 24 other protein kinases including PKBα and PDK1 with IC50 of >10 μM. SB 216763 stimulates glycogen synthesis in human liver cells with EC50 of 3.6 μM, and induces dose-dependent transcription of a β-catenin-LEF/TCF regulated reporter gene in HEK293 cells with a maximum 2.5-fold induction at 5 μM. [1] SB 216763 protects the cerebellar granule neurones from apoptotic cell death induced by LY-294002 or potassium-deprivation in a concentration-dependent manner, with a maximal neuroprotection at 3 μM in contrast with the effect of lithium chloride at which 10 mM is required. SB 216763 at 3 μM also completely prevents death of chicken dorsal root ganglion sensory neurones induced by LY-294002 regardless of NGF. SB 216763 treatment at 5 μM markedly inhibits the GSK-3-dependent phosphorylation of neuronal-specific microtubule-associated protein tau in cerebellar granule neurones or recombinant tau in HEK293 cells, and induces increased levels of cytoplasmic β-catenin in both cells mimicking the effect of Wnt-mediated inhibition of GSK-3. [2] In pancreatic cancer cell lines including BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC, SB 216763 treatment at 25-50 μM reduces cell viability in a dose-dependent manner, and leads to significant increase in apoptosis by 50% at 72 hours due to the specific down regulation of GSK-3β, while has no effect in HMEC or WI38 cell lines. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells MW\GeY5kfGmxbjDhd5NigQ>? NXfqfXZwT2y7Y3;n[Y4he3mwdHjhd4Uh[WO2aY\peJkhd2ZiSFXLNlk{KGOnbHzzMEBqdmirYnn0bY9vKG:oIFfTT|Mu[mW2YTygSWM2OD1yLkKg{txO MVSxNlk1OTN|MR?=
human HDLM-2 cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUDJcohq[mm2aX;uJI9nKGi3bXHuJGhFVE1vMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNlA6QDRizszN MVHTRW5ITVJ?
human NCI-H1770 cell MkjuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGLRNG9KdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVc4OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LjDJR|UxRTBwMkmzPVIh|ryP M{DvZ3NCVkeHUh?=
human HOP-62 cell M1Xo[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUnJcohq[mm2aX;uJI9nKGi3bXHuJGhQWC14MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPFUzPTZizszN M2LxUHNCVkeHUh?=
human JAR cell MmnES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWrS[3lIUW6qaXLpeIlwdiCxZjDoeY1idiCMQWKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlIyODR2IN88US=> MWPTRW5ITVJ?
human K5 cell M{fIS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGm2THNKdmirYnn0bY9vKG:oIHj1cYFvKEt3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJktKEmFNUC9NU42OzZ7ODFOwG0> MlruV2FPT0WU
human MV-4-11 cell NIfLZ4FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWnPc2ZtUW6qaXLpeIlwdiCxZjDoeY1idiCPVj20MVEyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi54OU[4OUDPxE1? NV3JemJCW0GQR1XS
human BT-549 cell MXnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX\rflhNUW6qaXLpeIlwdiCxZjDoeY1idiCEVD21OFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjJ7M{e2JO69VQ>? MU\TRW5ITVJ?
human LB2241-RCC cell NX;EcVFsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUPJcohq[mm2aX;uJI9nKGi3bXHuJGxDOjJ2MT3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03NjR{OEe0JO69VQ>? MljZV2FPT0WU
human GAMG cell NXjLWW9qT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWjJcohq[mm2aX;uJI9nKGi3bXHuJGdCVUdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14Lk[1OFA6KM7:TR?= NITS[4RUSU6JRWK=
human HMV-II cell MojKS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYnJcohq[mm2aX;uJI9nKGi3bXHuJGhOXi2LSTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuOlc3ODdizszN MorqV2FPT0WU
human RS4-11 cell MkPqS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MonGTY5pcWKrdHnvckBw\iCqdX3hckBTWzRvMUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlI1OTRzIN88US=> NEPKTJFUSU6JRWK=
human NCI-H1993 cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYXJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUm5N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvOzZ3M{Sg{txO MW\TRW5ITVJ?
human IST-SL1 cell M3e1NWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHPKN45KdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3TUFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF06NjB{MESg{txO NITMZ25USU6JRWK=
human SK-OV-3 cell MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYLUdoxmUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3PWk0{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QS5zMECyJO69VQ>? NHfOPZdUSU6JRWK=
human ESS-1 cell NWP4fGI4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIrsV49KdmirYnn0bY9vKG:oIHj1cYFvKEWVUz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU42QTh5MjFOwG0> MYjTRW5ITVJ?
human G-361 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH3OSWtKdmirYnn0bY9vKG:oIHj1cYFvKEdvM{[xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvOTd7ODFOwG0> MXHTRW5ITVJ?
human ALL-PO cell NVfZd4tHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4HoeGlvcGmkaYTpc44hd2ZiaIXtZY4hSUyOLWDPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvOzR5NzFOwG0> NVn2OW1mW0GQR1XS
human NB5 cell NGDiRnNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYXJcohq[mm2aX;uJI9nKGi3bXHuJG5DPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFzLkKxOVch|ryP Ml;oV2FPT0WU
human DU-145 cell M4XzfWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4HyUmlvcGmkaYTpc44hd2ZiaIXtZY4hTFVvMUS1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvPjV|NTFOwG0> M4jFTHNCVkeHUh?=
human NMC-G1 cell M33uVmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXvJcohq[mm2aX;uJI9nKGi3bXHuJG5OSy2JMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlczPjRizszN NYjZd2p2W0GQR1XS
human RPMI-7951 cell M1XRfWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXrJcohq[mm2aX;uJI9nKGi3bXHuJHJRVUlvN{m1NUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NiCLQ{WwQVEyNjd6NkSg{txO MoL4V2FPT0WU
human NCI-H2009 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXHJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkCwPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{NjJ2Mkeg{txO NX3ZZ|ZWW0GQR1XS
human LoVo cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NG\pUHZKdmirYnn0bY9vKG:oIHj1cYFvKEyxVn:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{4{PzdzIN88US=> MkTMV2FPT0WU
human A2058 cell MnS3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkToTY5pcWKrdHnvckBw\iCqdX3hckBCOjB3ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlQ3QSEQvF2= MWLTRW5ITVJ?
human D-566MG cell MlniS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3i1O2lvcGmkaYTpc44hd2ZiaIXtZY4hTC13Nk\NS{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{Njl2OEGg{txO MX3TRW5ITVJ?
human QIMR-WIL cell MnW5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXTJcohq[mm2aX;uJI9nKGi3bXHuJHFKVVJvV1nMJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvPjN7NDFOwG0> MljEV2FPT0WU
human S-117 cell M3zvb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NETNNZpKdmirYnn0bY9vKG:oIHj1cYFvKFNvMUG3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVYvPDB{MjFOwG0> MXrTRW5ITVJ?
human YH-13 cell NGT1N|JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIW2NWRKdmirYnn0bY9vKG:oIHj1cYFvKFmKLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVYvPjh4NTFOwG0> MlnYV2FPT0WU
human IGROV-1 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEnmPYNKdmirYnn0bY9vKG:oIHj1cYFvKEmJUl;WMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPy5{MECxJO69VQ>? NFv4[IdUSU6JRWK=
human BHT-101 cell M1XCSWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFK2PWFKdmirYnn0bY9vKG:oIHj1cYFvKEKKVD2xNFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPy54MEm5JO69VQ>? NWG4c45uW0GQR1XS
human MKN45 cell NILyU4FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mo\vTY5pcWKrdHnvckBw\iCqdX3hckBOU052NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG4MlQyOjhizszN MUfTRW5ITVJ?
human A498 cell NVLGTXVxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV\Jcohq[mm2aX;uJI9nKGi3bXHuJGE1QThiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zOT60OVQ6KM7:TR?= MWPTRW5ITVJ?
human U-266 cell NEfieIVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUfJcohq[mm2aX;uJI9nKGi3bXHuJHUuOjZ4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkCuPFc6PyEQvF2= M{X3OXNCVkeHUh?=
human BFTC-905 cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXrJcohq[mm2aX;uJI9nKGi3bXHuJGJHXENvOUC1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlEvOTh5OTFOwG0> MWLTRW5ITVJ?
human BxPC-3 cell M{PHOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFyxTopKdmirYnn0bY9vKG:oIHj1cYFvKEK6UFOtN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIyNjN3NUSg{txO MUjTRW5ITVJ?
human NCI-SNU-1 cell MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1zKR2lvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLWPOWU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjFwNUW1PEDPxE1? M1K2OXNCVkeHUh?=
human SF539 cell NH;yeppIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVLJcohq[mm2aX;uJI9nKGi3bXHuJHNHPTN7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkKuNFE6QCEQvF2= MVzTRW5ITVJ?
human VA-ES-BJ cell NVq0flhoT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MonZTY5pcWKrdHnvckBw\iCqdX3hckBXSS2HUz3CTkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIzNjV5NkOg{txO M3rwZXNCVkeHUh?=
human HuO-3N1 cell NV\sZ2VoT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGPqOllKdmirYnn0bY9vKG:oIHj1cYFvKEi3Tz2zUlEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOi54MUWxJO69VQ>? Mon3V2FPT0WU
human EM-2 cell NH6zUIpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MULJcohq[mm2aX;uJI9nKGi3bXHuJGVONTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{Mj62Olg6KM7:TR?= NYS5OVJ5W0GQR1XS
human T98G cell NIPnZXFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYDHd3F7UW6qaXLpeIlwdiCxZjDoeY1idiCWOUjHJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlMvOzByOTFOwG0> NGTQfVhUSU6JRWK=
human SW1783 cell NFSyT5hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFW2ZXZKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUe4N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI{NjV{NEOg{txO MkjnV2FPT0WU
human NKM-1 cell MnXLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX3Jcohq[mm2aX;uJI9nKGi3bXHuJG5MVS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkOuPFUyOiEQvF2= M1HFbHNCVkeHUh?=
human KOSC-2 cell NFL4VlNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUTJcohq[mm2aX;uJI9nKGi3bXHuJGtQW0NvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK0MlEzQTJizszN MmO3V2FPT0WU
human SW48 cell MnfuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NH:xNGxKdmirYnn0bY9vKG:oIHj1cYFvKFOZNEigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOE43ODZ5IN88US=> NYH3dHFKW0GQR1XS
human MIAPaCa2 cells MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV;uVWZmPzJiaB?= NIDDTnlIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBOUUGSYVPhNkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTTJIF{e2G7LDDJR|UxRTJ3IN88US=> M3vOclE6OzN6M{W1
human NCI-H28 cell M136eGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MX3Jcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOU4zOTB2IN88US=> NGDRTHFUSU6JRWK=
human 5637 cell NWfEZ3kzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUT3N2JPUW6qaXLpeIlwdiCxZjDoeY1idiB3NkO3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlUvQDFzMTFOwG0> MlfqV2FPT0WU
human NB69 cell M4DCOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{TFU2lvcGmkaYTpc44hd2ZiaIXtZY4hVkJ4OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK2MlE4PjRizszN MknMV2FPT0WU
human HT-29 cell MYLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYD6Om9ZUW6qaXLpeIlwdiCxZjDoeY1idiCKVD2yPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI4NjF5NUKg{txO MkPuV2FPT0WU
human PFSK-1 cell NHvTW4NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV\Jcohq[mm2aX;uJI9nKGi3bXHuJHBHW0tvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK3MlU6OyEQvF2= M37qNHNCVkeHUh?=
human UACC-257 cell MnvXS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NITucGNKdmirYnn0bY9vKG:oIHj1cYFvKFWDQ1OtNlU4KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjdwN{SzJO69VQ>? MmjhV2FPT0WU
human D-423MG cell NV:4dIVjT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYjJcohq[mm2aX;uJI9nKGi3bXHuJGQuPDJ|TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yO{46OTB4IN88US=> NYe1fnZjW0GQR1XS
human NH-12 cell NX\BSWpFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1P5OGlvcGmkaYTpc44hd2ZiaIXtZY4hVkhvMUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yPE41ODV7IN88US=> NI\ZSoNUSU6JRWK=
human DEL cell NVm1UGxIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1Hoc2lvcGmkaYTpc44hd2ZiaIXtZY4hTEWOIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkmuNFQ4PCEQvF2= NVPyTlJMW0GQR1XS
human LS-513 cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MX3Jcohq[mm2aX;uJI9nKGi3bXHuJGxUNTVzMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOwMlE{OzRizszN MoHJV2FPT0WU
human NBsusSR cell M1LUb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHjmWFJKdmirYnn0bY9vKG:oIHj1cYFvKE6Ec4XzV3Ih[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{OC53Nke4JO69VQ>? NH[0XXNUSU6JRWK=
human BV-173 cell M3TZOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFvOPIJKdmirYnn0bY9vKG:oIHj1cYFvKEKYLUG3N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMxNjd4NEmg{txO NYLZbnd7W0GQR1XS
human A101D cell NXvBZ|RvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M13OXmlvcGmkaYTpc44hd2ZiaIXtZY4hSTFyMVSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zNE46Pzh2IN88US=> M{myW3NCVkeHUh?=
human LU-134-A cell MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3vUWmlvcGmkaYTpc44hd2ZiaIXtZY4hVFVvMUO0MWEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{OS5yMkO4JO69VQ>? MnHSV2FPT0WU
human ES3 cell MnizS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVjJcohq[mm2aX;uJI9nKGi3bXHuJGVUOyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTNzLkOzO|Yh|ryP Ml;1V2FPT0WU
human NY cell M{S0Zmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFj0OpJKdmirYnn0bY9vKG:oIHj1cYFvKE6\IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{KuNlk3PSEQvF2= M2jy[HNCVkeHUh?=
human NCI-H1975 cell NV33WpRTT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NU\GT3ExUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE6PzViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|Mj6zOVgzKM7:TR?= MX7TRW5ITVJ?
human A704 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NInENnRKdmirYnn0bY9vKG:oIHj1cYFvKEF5MESgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zOE4zODV7IN88US=> NGXvTGpUSU6JRWK=
human SK-MEL-24 cell NYC0eINJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoLvTY5pcWKrdHnvckBw\iCqdX3hckBUUy2PRVytNlQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{PC5{NUKzJO69VQ>? MYTTRW5ITVJ?
human SW1088 cell M3fwNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFfnb4JKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUC4PEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVM1NjN2NUKg{txO MnnqV2FPT0WU
human HepG2 cells M{T5N2Z2dmO2aX;uJIF{e2G7 NXHzbpN4OzRizszN NXPCSYxWOTJiaB?= MWPJcohq[mm2aX;uJI9nKEeVS{Pi[ZRiKGmwIHj1cYFvKEincFeyJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKHKjdHnvJI9nKHCqb4PwbI9zgWyjdHXkJGdUN0eVIHH0JFM1KHWPIHHmeIVzKDF{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= M4LielI2PDZ5MUWw
mouse 3T3L1 cells Mmm4SpVv[3Srb36gZZN{[Xl? MXizOEDPxE1? M3XX[|EzKGh? MlLOTY5kemWjc3WgbY4h\2y3Y3;z[UBqdmOxcoDvdoF1cW:wIHnuJI1wfXOnIEPUN2wyKGOnbHzzJIF1KDN2IIXNJIFnfGW{IEGyJIhzeyCkeTDncJVkd3OnIHHzd4F6 Mm\3NlU1PjdzNUC=
human ST14A cells MkTtSpVv[3Srb36gZZN{[Xl? MYq2JIg> M3TSUGlvcGmkaYTpc44hd2ZiR2PLMVMu[mW2YT3t[YRq[XSnZDDXcpQhe2mpbnHsbY5oKGmwIHj1cYFvKFOWMUTBJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGGlY4XteYxifGmxbjDv[kBj\XSjLXPhd4VqdiCjcn;1coQhdnWlbHX1d{Bi\nSncjC2JIhzeyCkeTDtbYNzd3Olb4DpZ{BidmGueYPpdy=> NYXoOVJGOjB5MEi5N|c>
human ReNcell VM cells NYL4Z3lyWHKxbHnm[ZJifGmxbjDhd5NigQ>? MVizJO69VQ>? M1rTRVczKGh? MY\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKnTnPlcIwhXk1iY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBifCB|IIXNJIFnfGW{IEeyJIhzew>? MnnNNlA4ODh7M{e=
human SK-MEL-1 cell M3:yT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUXjOYZ[UW6qaXLpeIlwdiCxZjDoeY1idiCVSz3NSWwuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5MEBKSzVyPUO0MlcyPCEQvF2= MkC4V2FPT0WU
human MOLT-4 cell MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYDTU2xSUW6qaXLpeIlwdiCxZjDoeY1idiCPT1zUMVQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Pi5zNE[3JO69VQ>? M{XwWXNCVkeHUh?=
human T47D cell MoTIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVHJcohq[mm2aX;uJI9nKGi3bXHuJHQ1P0RiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|Nz6xOlQ4KM7:TR?= M3faVnNCVkeHUh?=
human SW1710 cell MnzoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkLTTY5pcWKrdHnvckBw\iCqdX3hckBUXzF5MUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zO{4{PjB6IN88US=> MX7TRW5ITVJ?
human MKN7 cell MmHPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWXHR4RVUW6qaXLpeIlwdiCxZjDoeY1idiCPS163JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|gvODlyOTFOwG0> MUDTRW5ITVJ?
human CAL-72 cell MnK5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnHDTY5pcWKrdHnvckBw\iCqdX3hckBESUxvN{KgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPE4yOTd6IN88US=> M1G5UXNCVkeHUh?=
human AGS cell MkPxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MniyTY5pcWKrdHnvckBw\iCqdX3hckBCT1NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|OD6yNFM6KM7:TR?= M1rQSXNCVkeHUh?=
human BE-13 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHvJeIlKdmirYnn0bY9vKG:oIHj1cYFvKEKHLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|gvPzd6MzFOwG0> NWX0[GpDW0GQR1XS
human Calu-6 cell Mm\1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlLDTY5pcWKrdHnvckBw\iCqdX3hckBE[Wy3LU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPU43QTZ2IN88US=> NWjDW4Q5W0GQR1XS
human CAL-27 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mm\BTY5pcWKrdHnvckBw\iCqdX3hckBESUxvMkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20OE4xODV2IN88US=> MWPTRW5ITVJ?
human BB49-HNC cell NHnmdnVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlXCTY5pcWKrdHnvckBw\iCqdX3hckBDSjR7LVjOR{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQ1NjB{N{ig{txO NWHiOpFDW0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo Administration of SB 216763 at 20 mg/kg significantly prevents lung inflammation and the subsequent fibrosis in bleomycin (BLM)-induced pulmonary inflammation and fibrosis model in mice by significantly blocking the production of inflammatory cytokines MCP-1 and TNF-α by macrophages, and significantly improves the survival of BLM-treated mice. SB 216763 treatment causes a significant reduction in BLM-induced alveolitis by inhibiting alveolar epithelial cell damage. [4]

Protocol

Kinase Assay:[1]
+ Expand

GSK-3 activity assay:

GSK-3 kinase activity is measured, in the presence of various concentrations of SB 216763, in a reaction mixture containing final concentrations of 1 nM human GSK-3α, 50 mM MOPS pH 7.0, 0.2 mM EDTA, 10 mM Mg-acetate, 7.5 mM β-mercaptoethanol, 5% (w/v) glycerol, 0.01% (w/v) Tween-20, 10% (v/v) DMSO, and 28 μM GS-2 peptide substrate. The GS-2 peptide sequence corresponds to a region of glycogen synthase that is phosphorylated by GSK-3. The assay is initiated by the addition of 0.34 μCi [33P]γ-ATP. The total ATP concentration is 10 μM. Following 30 minutes incubation at room temperature the assay is stopped by the addition of one third assay volume of 2.5% (v/v) H3PO4 containing 21 mM ATP. Samples are spotted onto P30 phosphocellulose mats and washed six times in 0.5% (v/v) H3PO4. The filter mats are sealed into sample bags containing Wallac betaplate scintillation fluid. 33P incorporation into the substrate peptide is determined by counting the mats in a Wallac microbeta scintillation counter.
Cell Research:[3]
+ Expand
  • Cell lines: BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC cells
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24, 48, and 72 hours
  • Method: Cells are exposed to various concentrations of SB 216763 for 24, 48 and 72 hours. Relative cell viability is measured using the MTS assay. Apoptotic cells are determined by staining with Hoechst.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: C57BL/6N mice with lung inflammation and fibrosis induced by bleomycin (BLM)
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 20 mg/kg
  • Administration: Intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 23 mg/mL (61.95 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+corn oil
For best results, use promptly after mixing.
4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.22
Formula

C19H12N2O2Cl2

CAS No. 280744-09-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

GSK-3 Signaling Pathway Map

GSK-3 Inhibitors with Unique Features

Related GSK-3 Products

Tags: buy SB216763 | SB216763 supplier | purchase SB216763 | SB216763 cost | SB216763 manufacturer | order SB216763 | SB216763 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID