Indirubin (NSC 105327)

Catalog No.S2386

For research use only.

Indirubin (NSC 105327) is a potent cyclin-dependent kinases and GSK-3β inhibitor with IC50 of about 5 μM and 0.6 μM.

Indirubin (NSC 105327) Chemical Structure

CAS No. 479-41-4

Selleck's Indirubin (NSC 105327) has been cited by 6 Publications

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Biological Activity

Description Indirubin (NSC 105327) is a potent cyclin-dependent kinases and GSK-3β inhibitor with IC50 of about 5 μM and 0.6 μM.
GSK-3β [2] CDK2/CyclinA [1] CDK5/p35 [1] CDK1/CyclinB [1] CDK4/CyclinD1 [1] Click to View More Targets
0.6 μM 2.2 μM 5.5 μM 10 μM 12 μM
In vitro

Indirubin is the active ingredient of Danggui Longhui Wan, a mixture of plants that is used in traditional Chinese medicine to treat chronic diseases. Indirubin inhibits CDKs activity with IC50 of 2.2 - 10 μM resulting in cell cycle arrest in the G2/M phase. [1] Indirubin also inhibits GSK-3β with an IC50 of 0.6 μM, attenuating CDK5- and GSK-3β-mediated tau phosphorylation, a process over-active in Alzheimer disease states. [2] It also suppresses tumor necrosis factor (TNF)-induced NF-κB activation in a dose- and time-dependent manner. Indirubin also suppresses the NF-κB activation induced by various inflammatory agents and carcinogens. Indirubin blocks the phosphorylation and degradation of IκBα through the inhibition of activation of IκBα kinase and phosphorylation and nuclear translocation of p65. [3]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 262.26


CAS No. 479-41-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC=C2C(=C1)C(=C(N2)O)C3=NC4=CC=CC=C4C3=O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02088281 Completed Drug: Indigo naturalis extract in oil ointment Psoriasis Vulgaris Chang Gung Memorial Hospital|National Science Council Taiwan November 2012 Phase 2

(data from, updated on 2022-08-01)

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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