CHIR-99021 (CT99021) HCl

Catalog No.S2924

CHIR-99021 (CT99021) HCl Chemical Structure

Molecular Weight(MW): 501.8

CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.

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Cited by 111 Publications

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. [1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). [2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
3T3-L1 MYrGeY5kfGmxbjDBd5NigQ>? MX6zJO69VQ>? NFnYRWE1KGh? MUXEUXNQ M1TLXWlvcGmkaYTzJJBz\WGmaYDvZ5l1\SCmaX\m[ZJmdnSrYYTpc44> MWqxNlA2PTJyMB?=
insulin receptor–expressing CHO-IR NFe0UW5HfW6ldHnvckBCe3OjeR?= Mmf4OVAxKG6P NF7wU5c{OCCvaX6= MYjEUXNQ NH;xZXZC[3SrdnH0[ZMhT2y7Y3;n[Y4hW3mwdHjhd4UhMEeVKR?= MV2xNlYxPjR7Nx?=
H4IIE NIrpcnVHfW6ldHnvckBCe3OjeR?= M2Pje|ExKM7:TR?= M{fNNVI1KGh? NF;ofoVFVVOR NIL2U5lT\We3bHH0[ZMh|rJvY3H0[Y5qdiCjY4Tpeol1gSCjbnSgWGlTTSClb370ZYlvcW6pIIDyc41wfGW{IHHjeIl3cXS7 NEDY[owyPTN3MEG5OS=>
Oct4/Sox2/Klf4-infected MEFs NX;YUItMTnWwY4Tpc44hSXO|YYm= MofGNVAh|ryP NYPrbG5OOjhiZB?= MWDEUXNQ NGPUTYNRem:vb4Tld{BOTU[|IILldJJw\3KjbX3pcochXHKjboPkeYNm\CCkeTDPZ5Q1NCCVb4iyMEBidmRiS3zmOC=> NGnISFMyQTh|OUC1OS=>
Sca-1+ HPC Mn[0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHztb44zKM7:TR?= MY[2JIQ> MWnEUXNQ MVXTfY5memerc4TpZ4FtdHlicILvcY91\XNiU3PhMVEsyqCKUFOgdJJwdGmoZYLheIlwdiCjbnSgZ49td267IH\vdo1ifGmxbjD0c4dmfGincjD3bZRpKEWJRh?= M2Oyb|IyPjJ2M{S4
HMC1.2 Ml;aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYKzNEDPxE1? MUG3NkBp NFXwXlFFVVOR MkPJVoVlfWOnczDj[YxtKHO3co\peoFt Mn\3NlIxOzl|MEG=
Endothelial cell(EC) NEHuVZJHfW6ldHnvckBCe3OjeR?= M1m2WVMh|ryP M{Xa[2ROW09? MX7Qdo9ud3SnczDlcoRwfGinbHnhcEBk\WyuIDjFR{kh\GmoZnXy[Y51cWG2aX;u NULuXnpwOjJyNUKwNFY>
PANC1 NEHySnVCeG:ydH;zbZMhSXO|YYm= MnuyxsA2KM7:TR?= MnKwO|IhcA>? M{XIN2ROW09? MUPUdolo\2W{czDhckBieG:ydH;0bYMhemW|cH;ud4U> M2Gx[lIzOjBzMUi2
INS-1E NYq2U3N3TnWwY4Tpc44hSXO|YYm= Mk\KNk42KM7:TR?= MUGyOEBp MkPYSG1UVw>? NYrwPWFLUW6mdXPld{BiKHKnZIXjeIlwdiCxZjDOVFEhd3[ncnX4dJJme3Orb36= NWq3OW1IOjJ2Mke3NFQ>
hiPSC-NPs NEj1Zo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUSz5qCK|ryP NEnQOXA4OiCq NH7zdoRFVVOR NUe1e|JqUW6lcnXhd4V{KGOnbHygdJJwdGmoZYLheIlwdsLi NYjicoRvOjJ4NEK2PFc>
platelets NXHOSVhuTnWwY4Tpc44hSXO|YYm= NI\NWlIyOCEQvF2= NWWyPHZnOTVibXnu MV\EUXNQ MXrJcoNz\WG|ZYOgeIhzd22kaX6tcYVlcWG2ZXSgdIxifGWuZYSgZYdoemWpYYTpc44h[W6mIFHUVEB{\WO{ZYTpc44> MVOyN|I{QTh5Nx?=
DN3 M{nhc2Z2dmO2aX;uJGF{e2G7 Mm\NNVAh|ryP Ml73PVYhcA>? M{H3N2ROW09? Mn\oTY5kemWjc3XzJINmdGxiZHX2[YxweG2nboSgZY5lKHC{b3zp[oVz[XSrb36= NIjnb2IzOzV{Nkm4PS=>
embryonic stem cells (ESC) M2TVfGZ2dmO2aX;uJGF{e2G7 MYWzJOK2VQ>? M1TzcFEyKGR? NETxRmtFVVOR MojQVJJwdW:2ZYOgd4Vt\i2{ZX7le4FtKG:oIH7hx893\S22eYDlJI1wfXOnIHXtZpJ6d26rYzDzeIVuKGOnbHzzJEhGW0Nr MXuyN|g1OzNzMh?=
H460 M1m4e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;zOUDDvU1? NULM[llbOjRiaB?= NWDkOmpJTE2VTx?= Mkj2VJJwdW:2ZYOgZ4VtdCCycn;sbYZmemG2aX;u NGX1cmIzPDN4N{[4PC=>
H157 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIrWRXQ2KML3TR?= MXKyOEBp NULaTpdiTE2VTx?= MUfQdo9ud3SnczDj[YxtKHC{b3zp[oVz[XSrb36= NIO3eHczPDN4N{[4PC=>
H460 M3uyPWFxd3C2b4Ppd{BCe3OjeR?= NU[4cI15PSEEtV2= MYGyOEBp MVrEUXNQ MnvURZR1\W63YYTld{Bk\WyuIHHwc5B1d3Orcx?= NHrOblQzPDN4N{[4PC=>
H157 NGrHZZVCeG:ydH;zbZMhSXO|YYm= M33pS|UhyrWP MkCyNlQhcA>? NY\QUmdkTE2VTx?= M2XQNWRw\XNibn;0JJNq\26rZnnjZY51dHliYX\m[YN1KGGyb4D0c5Nqew>? MVSyOFM3PzZ6OB?=
A549 NUfOUphkSXCxcITvd4l{KEG|c3H5 M4OyZlUhyrWP NVfQRnNxOjRiaB?= MkLaSG1UVw>? NHzS[pVKdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYO= Mo\hNlQ{Pjd4OEi=
Neural progenitor cells NWDx[XhqTnWwY4Tpc44hSXO|YYm= NWnTe|lXOyEQvF2= MUWxNEBl NYPQbmd6TE2VTx?= M1nqT2lv\HWlZYOg[4Vv\XKjdHnvckBw\iCwZYXyZYwheHKxZ3XubZRweiClZXzsdy=> Mnu0NlQ3OzhyM{S=
ES-D3 MnvZR5l1d3SxeHnjJGF{e2G7 MV2xNOKh|ryP M3uyVlMh\A>? NXrBTZVOTE2VTx?= MVTJcoR2[2W|IH3v[IVz[XSnIHP5eI91d3irY3n0fUB4cXSqIFnDOVAhd2ZiND65JO69VQ>? MmrxNlQ4Pzl|NkW=
ES-CCE M4PXVmN6fG:2b4jpZ{BCe3OjeR?= MmT1NVDDqM7:TR?= NI\DN2M{KGR? MUnEUXNQ MoHaTY5lfWOnczDtc4RmemG2ZTDjfZRwfG:6aXPpeJk> M2PORVI1Pzd7M{[1
ES-D3 MmfWSpVv[3Srb36gRZN{[Xl? NHLrVHg2yqEQvF2= M4\OPVMh\A>? MXrEUXNQ MYrTeJJwdmeueTDpcoR2[2W|IITo[UBi[3Srdnn0fUBw\iCZboSvZoV1[S2lYYTlcolvKHCjdHj3ZZk> NHLVXW0zPDd5OUO2OS=>
ES-CCE NVrYXllGTnWwY4Tpc44hSXO|YYm= MnH3OeKh|ryP NEXHeYY{KGR? M3;ueGROW09? NXP6V3RTW3S{b37ncJkhcW6mdXPld{B1cGViYXP0bZZqfHlib3[gW451N2KndHGtZ4F1\W6rbjDwZZRpf2G7 MVWyOFc4QTN4NR?=
SW982 NGr5UYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTBwMkG0NVYh|ryP NHHINY1USU6JRWK=
GCT MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2O4[GlEPTB;MD6zNFY4OiEQvF2= NVjZUHk5W0GQR1XS
769-P NECwZoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknlTWM2OD1yLkW4OFk6KM7:TR?= M{K5UHNCVkeHUh?=
LOXIMVI M2G5Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;Q[HRKSzVyPUCuOVg5OzJizszN NV\oNpFRW0GQR1XS
D-336MG NFnueXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRTBwOES2NkDPxE1? NGHRdpdUSU6JRWK=
S-117 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnK5TWM2OD1yLkmyNVMyKM7:TR?= M2[3SnNCVkeHUh?=
D-247MG NFHrZYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTBwOUS4OVIh|ryP NUjkOZdzW0GQR1XS
TYK-nu MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XXZmlEPTB;MT6wO|kyOiEQvF2= M{DD[3NCVkeHUh?=
A498 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnzjTWM2OD1zLkOwOFY6KM7:TR?= M{HjbHNCVkeHUh?=
BCPAP NFuyPVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3X5WWlEPTB;MT62Olg6QCEQvF2= MYfTRW5ITVJ?
KYSE-180 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LuWGlEPTB;MT63Nlc5QCEQvF2= NIjBN|FUSU6JRWK=
MIA-PaCa-2 NYTzdZEzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4WzbGlEPTB;MT63OVk4PyEQvF2= MoXsV2FPT0WU
HCC1395 MojkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIC2b41KSzVyPUGuO|gzPzNizszN MlXJV2FPT0WU
RS4-11 M2C3UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTFwOUW0NVUh|ryP NX[3NmppW0GQR1XS
NCI-H2122 MmTtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrSSGNvUUN3ME2yMlI4Pjh2IN88US=> MoC1V2FPT0WU
SNU-423 NHTxV2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHHC[VBKSzVyPUKuOFk4QDdizszN MVrTRW5ITVJ?
SK-LMS-1 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoH2TWM2OD1{Lk[0NVI5KM7:TR?= MkTxV2FPT0WU
RPMI-7951 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrhVoluUUN3ME2yMlcxOTl|IN88US=> MVrTRW5ITVJ?
COLO-829 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTJwN{[1OEDPxE1? NXHjSFBHW0GQR1XS
SCC-9 NXvZXYpjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn35TWM2OD1{Lkm1OFg2KM7:TR?= MVzTRW5ITVJ?
A704 NX3wb3lpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4HaeWlEPTB;Mz6wPFEzKM7:TR?= Mmj5V2FPT0WU
HOP-62 MnnVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;4O2tKSzVyPUOuN|YxPTRizszN Mmj1V2FPT0WU
HCC1569 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVHOU49NUUN3ME2zMlQ5OzB3IN88US=> MWXTRW5ITVJ?
A172 NV3LbmlpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2ThOWlEPTB;Mz63OVQ6QCEQvF2= MXfTRW5ITVJ?
MOLT-16 M1rqSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGDoT3NKSzVyPUOuPFg2PTZizszN MmLkV2FPT0WU
TE-15 NYjrc3hkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXjJR|UxRTNwOUWzOFMh|ryP Mn7uV2FPT0WU
OE19 MljoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHoUoRKSzVyPUOuPVY1PTFizszN NWPlVW1OW0GQR1XS
MHH-ES-1 MomzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3fCXWlEPTB;ND6wOFU1PSEQvF2= NVfEWXp1W0GQR1XS
NKM-1 MoHoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rJTGlEPTB;ND6xN|k4OSEQvF2= M{SyWnNCVkeHUh?=
RCC10RGB MoPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37LZ2lEPTB;ND6yPVQ4QSEQvF2= M{[4SHNCVkeHUh?=
BxPC-3 M2LINGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHC[pVGUUN3ME20MlcxOzlzIN88US=> MYLTRW5ITVJ?
ALL-PO NI\NNG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXNTWM2OD12LkizPFU5KM7:TR?= NFPUWZZUSU6JRWK=
SK-OV-3 MmTGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGXSfoNKSzVyPUSuPVk{PCEQvF2= M3HZVnNCVkeHUh?=
SW1710 M4DyfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTVwNEK0OVYh|ryP NFPJRnRUSU6JRWK=
NCI-H2452 NHrnTJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF33XFJKSzVyPUWuOFU3OjhizszN MYrTRW5ITVJ?
D-392MG M3i3Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXHhfWRxUUN3ME21MlQ6OTR3IN88US=> NHLJcZVUSU6JRWK=
LB2518-MEL Mn;YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUL1dGFjUUN3ME21MlczPjJ5IN88US=> MmDFV2FPT0WU
BL-41 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\TTHFLUUN3ME21MlgyOjZ4IN88US=> MWHTRW5ITVJ?
WM-115 NFrnNFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTVwOEG1NFkh|ryP NWXXVJpuW0GQR1XS
SK-MEL-2 MmDoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2T5XGlEPTB;Nj6zPFM1PCEQvF2= NVrheIljW0GQR1XS
CGTH-W-1 MkS1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\PcmJ5UUN3ME22MlQyPyEQvF2= M{DYc3NCVkeHUh?=
BB65-RCC NH\2N2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn:0TWM2OD14LkWzNVk2KM7:TR?= NUDab5pLW0GQR1XS
CAL-85-1 M3LRdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M33TSmlEPTB;Nj62NFk{PiEQvF2= M4PzcHNCVkeHUh?=
RT-112 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkfOTWM2OD15LkG2OFU3KM7:TR?= NVHsNXJZW0GQR1XS
MC116 MlXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NETBeo5KSzVyPUeuNlg3QTFizszN M2CwNnNCVkeHUh?=
G-361 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;ne|dKSzVyPUeuOFY2PjFizszN NELP[W9USU6JRWK=
SW1417 NXXJNpA1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTdwNkS1PFQh|ryP MVPTRW5ITVJ?
MV-4-11 Mn;CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlPvTWM2OD16LkW0N|A1KM7:TR?= MojZV2FPT0WU
CAL-72 NE\CR25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRThwN{[xNVMh|ryP NF2zVldUSU6JRWK=
MKN28 NVrTZVN4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYDpeIJmUUN3ME24Mlg5PTF4IN88US=> NHz3e4xUSU6JRWK=
MEG-01 NUL5TZJuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPOdlZKSzVyPUiuPVkxPDNizszN MnTMV2FPT0WU
ACHN M2izbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\wVmlEPTB;OT6zNFU1PiEQvF2= MojzV2FPT0WU
NCI-H1755 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTlwM{m5PFYh|ryP NH7Ve5BUSU6JRWK=
NCI-H64 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjDcFdsUUN3ME25MlQzOjV3IN88US=> M2nLO3NCVkeHUh?=
COLO-205 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;oNJZxUUN3ME25MlQ{QDJzIN88US=> NGDUPFFUSU6JRWK=
CTV-1 M4nqbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXz3XHJRUUN3ME25MlQ{QDl2IN88US=> M3HIb3NCVkeHUh?=
U-2-OS NXm5V4s6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF[zNmVKSzVyPUmuOFUxODZizszN MUPTRW5ITVJ?
LB996-RCC MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;yVYNwUUN3ME25MlUzOzl5IN88US=> M4Hj[nNCVkeHUh?=
SK-N-DZ Moe0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jqemlEPTB;OT62OVc1OiEQvF2= MnvQV2FPT0WU
EFM-19 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTlwN{W1OlYh|ryP MYLTRW5ITVJ?
DBTRG-05MG NGWxe4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHzjPZRKSzVyPUmuO|c4PyEQvF2= MUPTRW5ITVJ?
U-118-MG MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4LEeWlEPTB;OT65OFQ1PCEQvF2= NX;VSYRMW0GQR1XS
LB1047-RCC M{OwcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnaTWM2OD17Lkm5O|k5KM7:TR?= NYHmb3RwW0GQR1XS
NCI-H28 M{WxV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoHBTWM2OD1zMD60OFE{KM7:TR?= MV7TRW5ITVJ?
MZ2-MEL MlrkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTFyLkS2O|ch|ryP MmnxV2FPT0WU
M059J MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTFyLk[xNFIh|ryP NXLVb2xQW0GQR1XS
HT-3 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHRWHdKSzVyPUGwMlYyPzlizszN NXjVc4JPW0GQR1XS
SNU-387 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3fmU2lEPTB;MUCuO|I{PSEQvF2= NF64Wo5USU6JRWK=
NCI-H526 NF21TYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHtTWM2OD1zMT6xN|Y2KM7:TR?= M1fUfXNCVkeHUh?=
HEL MkTmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFfSR3pKSzVyPUGxMlY2PzlizszN NIX4W|BUSU6JRWK=
PA-1 NUTFOGZHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnPETWM2OD1zMT65N{DPxE1? Ml61V2FPT0WU
PC-14 NUnycHBFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTF{LkOyOEDPxE1? NF7KdoZUSU6JRWK=
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NCI-H2405 NFLUcHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHTwTFlKSzVyPUW2MlE3QTNizszN MXPTRW5ITVJ?
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MFE-296 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTZ2LkS0O|Mh|ryP MX\TRW5ITVJ?
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TE-12 MnvVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTh7LkO3OFEh|ryP M3rObHNCVkeHUh?=
NCI-H650 NYLXepVrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{Gy[2lEPTB;OEmuPFczPCEQvF2= Mn;1V2FPT0WU
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CAL-33 NHHuOGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Moi1TWM2OD17Mz6wOlAyKM7:TR?= MnfBV2FPT0WU
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MEL-JUSO Mlr0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGf6e4dKSzVyPUm0Mlk2PCEQvF2= M3HYOHNCVkeHUh?=
RMG-I NFvqN2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTl3LkGwO|Ih|ryP NFPFeGNUSU6JRWK=
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Caov-4 M1eyb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTl3Lk[4OUDPxE1? MlXsV2FPT0WU
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NCI-H292 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1P1WGlEPTB;OUmuN|AxPCEQvF2= NWKyU2tyW0GQR1XS
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KE-37 NV3CVWpET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVjoeVd3UUN3ME2xNFIvQTVizszN MVnTRW5ITVJ?
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PANC-08-13 MlW2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnrRTWM2OD1zME[uPFUh|ryP NHvO[3BUSU6JRWK=
NCI-H23 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTFyOD6zOVch|ryP NHzOdGFUSU6JRWK=
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D-263MG MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojpTWM2OD1zMEiuPVYyKM7:TR?= MVXTRW5ITVJ?
DU-145 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxRTFyOT65OFUh|ryP NGnOUVRUSU6JRWK=
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WSU-NHL M2SyZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTFzMj6wNUDPxE1? M1fse3NCVkeHUh?=
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MDA-MB-157 NUTrSlJ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2fDN2lEPTB;MUGyMlQ6KM7:TR?= NIjlemVUSU6JRWK=
NEC8 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;oTWM2OD1zMUKuOlI1KM7:TR?= MnnKV2FPT0WU
SIG-M5 M1Pv[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX[5VnZUUUN3ME2xNVMvOTJ3IN88US=> MnjnV2FPT0WU
786-0 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWTJR|UxRTFzMz60OVMh|ryP MlPyV2FPT0WU
DMS-273 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\OOopLUUN3ME2xNVUvPjF5IN88US=> M3f0[HNCVkeHUh?=
Calu-6 NUP0eph{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEDYbpFKSzVyPUGxOk44QThizszN NXvRU29CW0GQR1XS
HCC1599 NV\HcIdrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2WwUmlEPTB;MUG3MlE2PiEQvF2= NIfQPHlUSU6JRWK=
OVCAR-8 M2n2OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHPyV|BKSzVyPUGxO{43PjhizszN NXzTdJU{W0GQR1XS
ATN-1 NGmxclZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTFzNz64Nlgh|ryP MmHPV2FPT0WU
RKO MoXNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHj5[oZKSzVyPUGxO{45QThizszN NHLab3pUSU6JRWK=
SKG-IIIa NGCyVVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MliwTWM2OD1zMUiuNVE3KM7:TR?= NHzudVlUSU6JRWK=
SUP-T1 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{PwbWlEPTB;MUG4Mlk2PiEQvF2= MlLPV2FPT0WU
KNS-81-FD M{HTPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVLre|dJUUN3ME2xNVkvPDF5IN88US=> NH3xem9USU6JRWK=
OVCAR-3 NVK2SYMzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTFzOT65NVUh|ryP NVTu[I4{W0GQR1XS
NCI-N87 NHjCeIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\XTWM2OD1zMkGuNFQ1KM7:TR?= MmjsV2FPT0WU
HuP-T4 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWW1fpNRUUN3ME2xNlEvPTVizszN MVjTRW5ITVJ?
KYSE-140 NIrRboRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnyZZVWUUN3ME2xNlIvOTRzIN88US=> MlLqV2FPT0WU
HAL-01 NYPr[Yl7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPaPZFKSzVyPUGyNk41QTlizszN NXn0[JN1W0GQR1XS
HT-29 M4LoXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\2TGlEPTB;MUKyMlc4QCEQvF2= MmrWV2FPT0WU
OPM-2 M3nGXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPqTVFWUUN3ME2xNlMvOTd4IN88US=> NFz2UolUSU6JRWK=
SW1463 M1P5Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml7rTWM2OD1zMkSuNFc4KM7:TR?= NYTOXm11W0GQR1XS
NCI-H2087 MlPQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPGSJZKSzVyPUGyOE4zQDNizszN MknDV2FPT0WU
UMC-11 NH7qZ4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzITWM2OD1zMk[uNFM5KM7:TR?= MWHTRW5ITVJ?
EB-3 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjZOINqUUN3ME2xNlcvODF|IN88US=> M2DKWnNCVkeHUh?=
HEC-1 MnXsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYTJR|UxRTF{OT6xPVgh|ryP NH;pNZhUSU6JRWK=
LU-99A M2LofGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2TwPGlEPTB;MUK5Mlk{OSEQvF2= NF;NdXJUSU6JRWK=
VMRC-RCZ NHjY[oFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTF|MD6zO|Uh|ryP NEnh[2VUSU6JRWK=
MFH-ino MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDqeXZnUUN3ME2xN|EvOTF2IN88US=> MmLWV2FPT0WU
HT-1080 MmfTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2[2dWlEPTB;MUOyMlIxPCEQvF2= M1;KT3NCVkeHUh?=
CTB-1 NWS2XFBmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;aV|ZKSzVyPUGzNk45QTdizszN Mn75V2FPT0WU
Capan-2 M4C3Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mkn4TWM2OD1zM{OuPVc2KM7:TR?= MnvIV2FPT0WU
BB30-HNC MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTF|ND6xNVYh|ryP NYO0VFgyW0GQR1XS
CP50-MEL-B M{\PXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPab5NKSzVyPUGzOE4zKM7:TR?= NV;WZmY2W0GQR1XS
JEG-3 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jXWmlEPTB;MUO0MlM2QSEQvF2= NVfpUndnW0GQR1XS
NCI-H1437 M{HYU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTF|ND61PUDPxE1? MoGyV2FPT0WU
NOS-1 MoDiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzkWXBqUUN3ME2xN|QvPzZ3IN88US=> M4Th[HNCVkeHUh?=
G-402 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlrTTWM2OD1zM{WuNlk2KM7:TR?= NYDmXYpQW0GQR1XS
OAW-42 NF;TTpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jFSmlEPTB;MUO3Mlg2PiEQvF2= M1XGWXNCVkeHUh?=
Ca-Ski M2rWO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXuUpk4UUN3ME2xN|gvQDNzIN88US=> MlTqV2FPT0WU
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OVCAR-4 M4rMVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTF2MD63OFkh|ryP NEm1[|JUSU6JRWK=
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KP-4 MlzzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;zZ5NMUUN3ME2zOFMvPzN|IN88US=> MofIV2FPT0WU
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NB13 MkS4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDkXnA2UUN3ME2zOFUvPThizszN NV\TdZNYW0GQR1XS
D-502MG MmD1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1vrfGlEPTB;M{S1MlgyOiEQvF2= NVW0[YV3W0GQR1XS
CAL-54 NUPieVJsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{HtcmlEPTB;M{S1Mlg4QSEQvF2= M17pNHNCVkeHUh?=
DOHH-2 Ml3CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIq2XWRKSzVyPUO0Ok43PSEQvF2= Mnv0V2FPT0WU
SK-PN-DW MorJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnu2TWM2OD1|NEeuOFI3KM7:TR?= NHHRfHhUSU6JRWK=
NCI-H720 M4TmNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTN2OT64OFYh|ryP M3\2ZXNCVkeHUh?=
SW1990 M{HxT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYDGV4tRUUN3ME2zOVEvOjhizszN M2KzWnNCVkeHUh?=
NCI-H2196 NEnTXFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{LMe2lEPTB;M{WyMlYzPCEQvF2= NI\5Uo1USU6JRWK=
P30-OHK M4\uZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{XSPGlEPTB;M{WzMlA3OyEQvF2= MXnTRW5ITVJ?
NCI-H358 NXjndVNrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYX5bXVNUUN3ME2zOVUvOzV4IN88US=> M3XZR3NCVkeHUh?=
CW-2 NHjIUmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4rzc2lEPTB;M{W2MlUzQSEQvF2= M{jaSHNCVkeHUh?=
COLO-678 NHPYco9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTN3Nz64OlMh|ryP M4HHfXNCVkeHUh?=
NCI-H2126 Mn6xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPOdmRMUUN3ME2zOVgvPTJzIN88US=> NYP2SHVUW0GQR1XS
DMS-53 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTN3OD63NVEh|ryP M4PjdXNCVkeHUh?=
NCI-H1838 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4ryUWlEPTB;M{W5Mlk5PyEQvF2= NEfkSJlUSU6JRWK=
SJSA-1 Mn\GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnkW3hoUUN3ME2zOlAvQTN7IN88US=> M{\uSXNCVkeHUh?=
ChaGo-K-1 Ml;uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XFTmlEPTB;M{[zMlkzPCEQvF2= M3;KVnNCVkeHUh?=
IST-SL2 NIjhUnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjsXll7UUN3ME2zO|IvQTJ4IN88US=> MkXVV2FPT0WU
U-266 NH\o[WZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2nLdmlEPTB;M{ezMlE{OiEQvF2= M1TaR3NCVkeHUh?=
P31-FUJ NFzLV4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M164fmlEPTB;M{e2Mlk3PCEQvF2= NH;YWohUSU6JRWK=
HCC2157 M4PsR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTN5OD6xOlEh|ryP M2X0[3NCVkeHUh?=
COR-L88 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFzaNGlKSzVyPUO4N{43PzdizszN MWrTRW5ITVJ?
NCI-H596 NV\3RZNjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIrK[3JKSzVyPUO4OU4{PCEQvF2= Mn;UV2FPT0WU
GOTO MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jFN2lEPTB;M{mwMlY1OSEQvF2= NUjqTpViW0GQR1XS
NCI-H719 MlX1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjYfGU1UUN3ME2zPVEvQDl|IN88US=> MU\TRW5ITVJ?
MN-60 NEPFSG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUXufmE1UUN3ME2zPVMvQTR6IN88US=> Mn3xV2FPT0WU
IST-SL1 NEnP[4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTN7ND60NFYh|ryP M3TYOnNCVkeHUh?=
TE-6 NYXIc|lNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1jyVGlEPTB;M{m3MlI2PCEQvF2= NITsWnBUSU6JRWK=
SW962 M1W0XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jyTmlEPTB;M{m4MlY6KM7:TR?= MmfjV2FPT0WU
FADU Mm[yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTrZZc4UUN3ME20NFMvODh|IN88US=> MWDTRW5ITVJ?
ECC4 M3nF[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlH4TWM2OD12MEiuOFU5KM7:TR?= M4DyUXNCVkeHUh?=
MES-SA NUPYXoJDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWrsdHZzUUN3ME20NFkvQThizszN Mn7uV2FPT0WU
NCI-H716 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXTsN2pKUUN3ME20NVAvODd3IN88US=> NFrjd5lUSU6JRWK=
GR-ST MknYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHKx[IFKSzVyPUSxNE41QTdizszN MV\TRW5ITVJ?
KY821 Mn;TS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTRzND6wPFYh|ryP M3X1U3NCVkeHUh?=
NCI-H2141 NXroVGZ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXzJR|UxRTR{ND6zPFgh|ryP NHzHfmlUSU6JRWK=
MDA-MB-134-VI MkDoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4ns[WlEPTB;NEOxMlA{OyEQvF2= Mn;oV2FPT0WU
RH-1 MknhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3vTWM2OD12M{WuNFkh|ryP M17ET3NCVkeHUh?=
AM-38 M3zP[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVLJR|UxRTR|OD61OEDPxE1? M1y3ZnNCVkeHUh?=
BL-70 M2XU[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTR|OT60Nlkh|ryP MnvTV2FPT0WU
COLO-684 NIK2OGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjMTWM2OD12NEiuPFg5KM7:TR?= MXzTRW5ITVJ?
DMS-79 NIX6[pRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1rQd2lEPTB;NES5MlY5KM7:TR?= NVrNUnJOW0GQR1XS
EW-1 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxRTR3Mj64PFgh|ryP Mn;qV2FPT0WU
MS-1 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2rCZ2lEPTB;NEW0MlM5PSEQvF2= MWDTRW5ITVJ?
A4-Fuk NHf6eHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEX2OIZKSzVyPUS1OE42ODdizszN MlPDV2FPT0WU
TALL-1 NV\aOlFiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTEPYFKSzVyPUS1OU4zQTdizszN NHS1b|lUSU6JRWK=
SCLC-21H NFnKdnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTD[WVXUUN3ME20OVUvPjl7IN88US=> NXLHeZd4W0GQR1XS
KMS-12-PE M3Tifmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV\JR|UxRTR3Nj64NlIh|ryP M1LX[3NCVkeHUh?=
TE-441-T Mo\kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{n3eGlEPTB;NEW3MlE5PiEQvF2= NWK4W3h5W0GQR1XS
A3-KAW NGTJTWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MonRTWM2OD12NkCuPFQyKM7:TR?= MUfTRW5ITVJ?
NCI-H1092 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzNTWM2OD12NkOuPVQ{KM7:TR?= NUe0SVVXW0GQR1XS
COR-L279 NHzBV2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUX4b2VLUUN3ME20OlQvQDR5IN88US=> NVr0U41QW0GQR1XS
NCI-SNU-5 NHz5dmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmO2TWM2OD12N{KuNlgh|ryP M3;2[3NCVkeHUh?=
JAR MlG2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHTEeItKSzVyPUS3OE44QDhizszN NH;RTHFUSU6JRWK=
NCI-H446 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxRTR5Nz6wPEDPxE1? M17aTnNCVkeHUh?=
HDLM-2 MonXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{mxT2lEPTB;NEiwMlg3QSEQvF2= NFfWTXFUSU6JRWK=
D-283MED NUHOS2FZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{H1N2lEPTB;NEi4MlkzPCEQvF2= M3;RNXNCVkeHUh?=
NCI-H1395 NY\henA1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTR6OT6wNFQh|ryP MkfvV2FPT0WU
RPMI-6666 MmT0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jEdmlEPTB;NEmxMlM6QCEQvF2= NFPobZhUSU6JRWK=
CPC-N M2i0[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTVyMj6wOlMh|ryP MkXSV2FPT0WU
EC-GI-10 NYLLOWRCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXm[Il5UUN3ME21NFIvOTZ7IN88US=> NHG0dmJUSU6JRWK=
CA46 NXv1[YpXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUDJR|UxRTVyND60NFch|ryP NVvZXIJLW0GQR1XS
EHEB NIXsfotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoriTWM2OD13MEeuNlA5KM7:TR?= NW[4W4FUW0GQR1XS
RPMI-8402 MoD2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHrsbWpKSzVyPUWxNE4xOjFizszN M4fVWnNCVkeHUh?=
DU-4475 Mk\mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTrTWM2OD13MUGuNVAyKM7:TR?= M4jTVXNCVkeHUh?=
P12-ICHIKAWA MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvqTWM2OD13MUGuOFE3KM7:TR?= MWPTRW5ITVJ?
NCI-H1694 Mny3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXvpc3R3UUN3ME21NVIvPDN7IN88US=> NX:0PXNLW0GQR1XS
NCI-H2171 NGezcXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTVzMj63NFUh|ryP NV\SWmdFW0GQR1XS
Mo-T NWq1N|JpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWfh[457UUN3ME21NVUvOTF4IN88US=> NXKwTHpbW0GQR1XS
HL-60 NV;yS|FLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXTJSFRuUUN3ME21NlIvQTJ2IN88US=> NFLlS4tUSU6JRWK=
NCI-H1436 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH65d|RKSzVyPUWyN{4xPjRizszN MYfTRW5ITVJ?
NCI-H510A MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M33S[GlEPTB;NUK0MlY6PiEQvF2= NHLvUZVUSU6JRWK=
NCI-H2081 NGTFcodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEO4S|lKSzVyPUWyOU42OTNizszN Ml3vV2FPT0WU
NB5 NHrxTodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFLhOFlKSzVyPUWyOk41OyEQvF2= NWrrVJIzW0GQR1XS
NCI-H2227 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2jJfmlEPTB;NUOwMlI2PCEQvF2= NV72Vpg1W0GQR1XS
HSC-4 NXzERoxZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;wZ|BFUUN3ME21N|kvOjl|IN88US=> MnrlV2FPT0WU
RL Mn\lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTV2Mj60OFIh|ryP NEPJUXVUSU6JRWK=
EVSA-T MmPhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHu1UotKSzVyPUW0N{43QDRizszN NXj0fldbW0GQR1XS
NCI-H889 NFvuVWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWrJR|UxRTV2Nz6yN{DPxE1? MmX2V2FPT0WU
COLO-320-HSR NWjtfnVKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVTJR|UxRTV3MT6zOUDPxE1? M4\xZ3NCVkeHUh?=
EW-11 Mn\MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYH6VlFCUUN3ME21OVIvOzl7IN88US=> MkSwV2FPT0WU
UACC-812 NWXifWFET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYnJR|UxRTV3OT6zPVEh|ryP MoexV2FPT0WU
CAL-148 NF[zOoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUD2e2V1UUN3ME21OlUvODh7IN88US=> NV7pVGEzW0GQR1XS
K052 NF3MeXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPZfFdKSzVyPUW3PU4zOTNizszN M{fCd3NCVkeHUh?=
L-428 NFL1TVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4D3SmlEPTB;NkCwMlY4OSEQvF2= NEjWToVUSU6JRWK=
LP-1 M{XYRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHETnpKSzVyPU[wN{42QDdizszN NYfiU3NsW0GQR1XS
ML-2 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vWXGlEPTB;NkC2MlU4QSEQvF2= M2TYNnNCVkeHUh?=
KARPAS-45 MlnFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLETWM2OD14MUiuNlIyKM7:TR?= MnPrV2FPT0WU
HT NHnYPYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoXyTWM2OD14MkKuO|g2KM7:TR?= M1X2[nNCVkeHUh?=
NCI-H226 M1HrWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHjXcplKSzVyPU[yPE4{PTNizszN M2nnSXNCVkeHUh?=
L-363 MkPVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzn[2JCUUN3ME22OFgvODZ2IN88US=> NH72[5NUSU6JRWK=
IM-9 Mn;mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTZ3MT61OFEh|ryP Mmj4V2FPT0WU
TUR MkToS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF3yeXdKSzVyPU[3NU4{OjJizszN NVSzUYhKW0GQR1XS
NCI-SNU-16 NHPP[ppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3rtdGlEPTB;NkiwMlQ3OiEQvF2= MYLTRW5ITVJ?
NCI-SNU-1 MlfVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HYcWlEPTB;NkmwMlE4OiEQvF2= NFW1ZlBUSU6JRWK=
U-87-MG NV\OeFNUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml\qTWM2OD14OUSuOVg2KM7:TR?= NGXuUmNUSU6JRWK=
KM-H2 Mny0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4Tl[GlEPTB;Nkm5MlA2QSEQvF2= NEHYTZRUSU6JRWK=
DEL NEG2e3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUjMZXN4UUN3ME23NFAvQDN6IN88US=> NXj5PIxNW0GQR1XS
KARPAS-422 M1POUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLFUYZKSzVyPUe4PU4{OTVizszN MnP6V2FPT0WU
BC-3 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRThyNT6yNFMh|ryP NIfadmZUSU6JRWK=
NCI-H1155 M4PYSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLtTWM2OD16MUiuNFU6KM7:TR?= MXfTRW5ITVJ?
NCI-H1581 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\YTWM2OD17MECuNlMyKM7:TR?= NF\SRYVUSU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cleaved PARP / CD31 / VE-Cadherin / α-SMA / vimentin; 

PubMed: 30709379     


NCI-H460 cells were co-cultured with HUVECs in a 3D culture system, and then treated with 1 μM CHIR-99021, 10 μM or 20 μM Cisplatin, or a combination of both, for 24 h. Cells were harvested and immunoblotted with anti-cleaved PARP, anti-CD31, anti-VE-cadherin, anti-α-SMA, anti-vimentin, and anti-β-actin antibodies. 

CTNNB1; 

PubMed: 23993102     


Treatment with the GSK3β inhibitor CHIR-99021 led to increased levels of β-catenin in RKO and HT29 (non-mutant) cells.

β-catenin; 

PubMed: 24165128     


HeLa cells were treated with 5 μm CHIR-99021 for the indicated times. The samples were analyzed by Western blot.

30709379 23993102 24165128
Growth inhibition assay
Cell viability; 

PubMed: 22039301     


HMC-1.2 cells were cultured in the absence or presence of GSK3β inhibitor (CHIR 99021, 3–30 µM) and viability cell count was assessed by trypan blue dye exclusion after 24 h, 48 h or 72 h. All data are presented as the mean ± SEM of three independent experiments conducted in triplicate. p < 0.05*, p < 0.001** p < 0.0001*** vs vehicle treated control cells.

22039301
Immunofluorescence
SOX10 / EdU; 

PubMed: 28174705     


Incorporation of EdU (white arrowheads) into SOX10+ ENS (enteric nervous system) cells with and without CHIR-99021.

Oct3/4; 

PubMed: 24779365     


Fluorescence micrographs showing the protein expression of Brachyury/Oct3/4 after a three day differentiation of ES-D3 cells with BIO (0.5 μM), SB-216763 (5 μM), CHIR-99021 (5 μM), or CHIR-98014 (1 μM). Nuclei were counterstained with DAPI (blue). The merge is shown in yellow. Original magnification 400x. Scale bar = 50 μm.

28174705 24779365
In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. [1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. [4]

Protocol

Kinase Assay:

[1]

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Cell-free kinase assays:

Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research:

[3]

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  • Cell lines: INS-1E
  • Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM
  • Incubation Time: 1, or 4 days
  • Method:

    Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes
  • Formulation: Formulated as solutions in 20 mM citrate-buffered 15% Captisol or as fine suspensions in 0.5% carboxymethylcellulose.
  • Dosages: ~48 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (185.33 mM)
Ethanol 2 mg/mL (3.98 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
24mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 501.8
Formula

C22H18Cl2N8.HCl

CAS No. 1797989-42-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?

  • Answer:

    The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.

  • Question 2:

    How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?

  • Answer:

    Please change the culture medium each day to add the new comound in it if the duration is more than 72h.

  • Question 3:

    What is the difference between S2924 and S1263? Which one is better for differentiation?

  • Answer:

    S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID