Laduviglusib (CHIR-99021) HCl

Catalog No.S2924 Synonyms: CT99021 HCl

For research use only.

Laduviglusib (CHIR-99021; CT99021) HCl is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2. CHIR-99021 is a potent pharmacological activators of the Wnt/beta-catenin signaling pathway. CHIR-99021 significantly rescues light-induced autophagy and augments GR, RORα and autophagy-related proteins.

Laduviglusib (CHIR-99021) HCl Chemical Structure

CAS No. 1797989-42-4

Selleck's Laduviglusib (CHIR-99021) HCl has been cited by 469 publications

Purity & Quality Control

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Biological Activity

Description Laduviglusib (CHIR-99021; CT99021) HCl is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2. CHIR-99021 is a potent pharmacological activators of the Wnt/beta-catenin signaling pathway. CHIR-99021 significantly rescues light-induced autophagy and augments GR, RORα and autophagy-related proteins.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. [1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). [2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H1581 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTlyMD6yN|Eh|ryP NUfRc45CRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
NCI-H1155 M1q4fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRThzOD6wOVkh|ryP M{fIOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
BC-3 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\5TWM2OD16MEWuNlA{KM7:TR?= M{XocFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
KARPAS-422 NUnEfYkxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnnZ3lGUUN3ME23PFkvOzF3IN88US=> NUTmb2dxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
DEL NIPncnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrpTWM2OD15MECuPFM5KM7:TR?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
KM-H2 NVe4TodmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTZ7OT6wOVkh|ryP NVT6UZp{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
U-87-MG M131TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlWyTWM2OD14OUSuOVg2KM7:TR?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
NCI-SNU-1 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTZ7MD6xO|Ih|ryP MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
NCI-SNU-16 M1TlTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTZ6MD60OlIh|ryP M1jaNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
TUR Mkn6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHFd3F5UUN3ME22O|EvOzJ{IN88US=> MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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NCI-H226 NWLQZYFOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYfTTFk2UUN3ME22NlgvOzV|IN88US=> NED5TWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
HT MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWOxTm1GUUN3ME22NlIvPzh3IN88US=> NHyzUGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
KARPAS-45 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1HCRWlEPTB;NkG4MlIzOSEQvF2= M3f1ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
ML-2 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXvjelZbUUN3ME22NFYvPTd7IN88US=> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
LP-1 NVvkW3c3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPI[HBKSzVyPU[wN{42QDdizszN M4rrS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
L-428 M{DUc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkG5TWM2OD14MECuOlcyKM7:TR?= NWDucG4xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
K052 NVv0UYhFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXPpS5FCUUN3ME21O|kvOjF|IN88US=> NFWyTnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
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EW-11 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnmW|ZyUUN3ME21OVIvOzl7IN88US=> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
COLO-320-HSR NYDI[|AyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHKwO5pKSzVyPUW1NU4{PSEQvF2= NYrhbmpERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
NCI-H889 MnPvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlP2TWM2OD13NEeuNlMh|ryP M3W0OlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
EVSA-T NIHiVo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoW2TWM2OD13NEOuOlg1KM7:TR?= NIDQUWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
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NCI-H2081 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nkPWlEPTB;NUK1MlUyOyEQvF2= NYDIU2F{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
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MKN28 NVu2SIJiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3KSpVoUUN3ME24Mlg5PTF4IN88US=> NW\JVIk1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
CAL-72 NED6VmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHy3b25KSzVyPUiuO|YyOTNizszN MkDXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MV-4-11 M3G0TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfDVG9KSzVyPUiuOVQ{ODRizszN NIHNb4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
SW1417 NGTLbo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3jP[WlEPTB;Nz62OFU5PCEQvF2= NHL0N3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
G-361 M2j6Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXnTWM2OD15LkS2OVYyKM7:TR?= NH3sdoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
MC116 M4TuTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\TdWRKSzVyPUeuNlg3QTFizszN NUfkRotwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
RT-112 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIn5WIRKSzVyPUeuNVY1PTZizszN NEjmeII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
CAL-85-1 M4rBfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2ewd2lEPTB;Nj62NFk{PiEQvF2= MlK5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
BB65-RCC MnvPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnH3TWM2OD14LkWzNVk2KM7:TR?= NGP1SGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
CGTH-W-1 NHTsdYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2[zT2lEPTB;Nj60NVch|ryP MnTMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
WM-115 NVLtVGlqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLVdphKSzVyPUWuPFE2ODlizszN NHjjb|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
SK-MEL-2 NVrsNoZ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlXWTWM2OD14LkO4N|Q1KM7:TR?= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
BL-41 NHfDfHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTVwOEGyOlYh|ryP MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
LB2518-MEL NXP5T2ZRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWDJR|UxRTVwN{K2Nlch|ryP MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
D-392MG MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkToTWM2OD13LkS5NVQ2KM7:TR?= M3LndVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
NCI-H2452 NXXtSplST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\1TWM2OD13LkS1OlI5KM7:TR?= MmC0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
SW1710 M{TPW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXK4dnlFUUN3ME21MlQzPDV4IN88US=> Mn\JQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
SK-OV-3 NVft[mdjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPQTWM2OD12Lkm5N|Qh|ryP M1HjUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
ALL-PO NHvVelBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzXTWM2OD12LkizPFU5KM7:TR?= NXrDS5ZbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
BxPC-3 NYLJ[oJUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4XSUmlEPTB;ND63NFM6OSEQvF2= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
RCC10RGB NVTEOGl1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn\1TWM2OD12LkK5OFc6KM7:TR?= NHm0OpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
NKM-1 M{nlNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4e2W2lEPTB;ND6xN|k4OSEQvF2= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
MHH-ES-1 M{\BXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXkTWM2OD12LkC0OVQ2KM7:TR?= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
OE19 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPmUXZ7UUN3ME2zMlk3PDVzIN88US=> NILw[mI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
TE-15 Mkm4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37GSWlEPTB;Mz65OVM1OyEQvF2= NHT0R3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
MOLT-16 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoXxTWM2OD1|Lki4OVU3KM7:TR?= NXntNlRNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
A172 M{\GUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWX5NVdMUUN3ME2zMlc2PDl6IN88US=> NWnac3FORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
HCC1569 NVHmRnZVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVXJR|UxRTNwNEizNFUh|ryP NGDGVWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
HOP-62 M4HSSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XGfmlEPTB;Mz6zOlA2PCEQvF2= NEPz[HY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
A704 MofpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPrWVlKSzVyPUOuNFgyOiEQvF2= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
SCC-9 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPsTpZKSzVyPUKuPVU1QDVizszN NF:4U4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
COLO-829 M{jtS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTJwN{[1OEDPxE1? NGDNVVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
RPMI-7951 M3vSV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYW3SndGUUN3ME2yMlcxOTl|IN88US=> NGGwV|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
SK-LMS-1 M{H2bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jGWGlEPTB;Mj62OFEzQCEQvF2= NFXndJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
SNU-423 NYLuNVRIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDvTWM2OD1{LkS5O|g4KM7:TR?= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
NCI-H2122 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTJwMke2PFQh|ryP MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
RS4-11 NYrrPVRyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTFwOUW0NVUh|ryP NUHpN3BnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
HCC1395 M{LXcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV3KdmJPUUN3ME2xMlc5Ojd|IN88US=> NULiOXI4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
MIA-PaCa-2 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPCS4dtUUN3ME2xMlc2QTd5IN88US=> NHfNW5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
KYSE-180 NHzvVndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3f0bGlEPTB;MT63Nlc5QCEQvF2= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
BCPAP M{HKRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXX4U3k5UUN3ME2xMlY3QDl6IN88US=> NYnjeZpHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
A498 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;HbJRLUUN3ME2xMlMxPDZ7IN88US=> Mn6yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
TYK-nu NYTjcGU1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTFwMEe5NVIh|ryP MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
D-247MG M{\5N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTBwOUS4OVIh|ryP NFHVOVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
S-117 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTBwOUKxN|Eh|ryP NHjJc5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
D-336MG MkXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{nUeGlEPTB;MD64OFYzKM7:TR?= NH7kNo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
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769-P NECzdHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXrfJRKSzVyPUCuOVg1QTlizszN M3uwOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
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SW982 NXnUU4hNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmjzTWM2OD1yLkKxOFE3KM7:TR?= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
ES-CCE MUfGeY5kfGmxbjDBd5NigQ>? MmWwOeKh|ryP NXq4elBROyCm MX7EUXNQ MY\TeJJwdmeueTDpcoR2[2W|IITo[UBi[3Srdnn0fUBw\iCZboSvZoV1[S2lYYTlcolvKHCjdHj3ZZk> MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd5OUO2OUc,OjR5N{mzOlU9N2F-
ES-D3 NHHHPWpHfW6ldHnvckBCe3OjeR?= MXy1xsDPxE1? NHeycoU{KGR? M{CyO2ROW09? MkXhV5Rzd26pbImgbY5lfWOnczD0bIUh[WO2aY\peJkhd2ZiV370M4JmfGFvY3H0[Y5qdiCyYYToe4F6 NGTDS5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe3PVM3PSd-MkS3O|k{PjV:L3G+
ES-CCE NWHYO3h{S3m2b4TvfIlkKEG|c3H5 NEjhWWQyOMLizszN MV2zJIQ> MX\EUXNQ M3X5OGlv\HWlZYOgcY9l\XKjdHWgZ5l1d3SxeHnjbZR6 MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd5OUO2OUc,OjR5N{mzOlU9N2F-
ES-D3 MVPDfZRwfG:6aXOgRZN{[Xl? NF;xNXoyOMLizszN MkWzN{Bl M3K2WGROW09? NXHlbZJrUW6mdXPld{Bud2SncnH0[UBkgXSxdH;4bYNqfHlid3n0bEBKSzVyIH;mJFQvQSEQvF2= Mmr1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5N{mzOlUoRjJ2N{e5N|Y2RC:jPh?=
Neural progenitor cells NUDSVHA3TnWwY4Tpc44hSXO|YYm= NXy0cFlKOyEQvF2= NXmwbI1iOTBiZB?= MXHEUXNQ NW\BcWZ6UW6mdXPld{Bo\W6ncnH0bY9vKG:oIH7leZJidCCycn;n[Y5qfG:{IHPlcIx{ NVu3SGNiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N|gxOzRpPkK0OlM5ODN2PD;hQi=>
A549 M2DYZ2Fxd3C2b4Ppd{BCe3OjeR?= NX\rVodbPSEEtV2= NIDMU2szPCCq MkjZSG1UVw>? NGjiZnJKdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYO= NILPXFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2O|Y5QCd-MkSzOlc3QDh:L3G+
H157 M1yySWFxd3C2b4Ppd{BCe3OjeR?= NUP2e2tmPSEEtV2= MlS4NlQhcA>? MULEUXNQ NUnINGsyTG:nczDuc5Qhe2mpbnnmbYNidnSueTDh[oZm[3RiYYDvdJRwe2m| NWDzOHhoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlc3QDhpPkK0N|Y4Pjh6PD;hQi=>
H460 NULaNGxKSXCxcITvd4l{KEG|c3H5 NX\KfWt1PSEEtV2= NXW1NXlmOjRiaB?= NGPCZolFVVOR Mlz3RZR1\W63YYTld{Bk\WyuIHHwc5B1d3Orcx?= MorqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|Nke2PFgoRjJ2M{[3Olg5RC:jPh?=
H157 M1jHfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjZOUDDvU1? MYSyOEBp MmDhSG1UVw>? NHrYSmdRem:vb4Tld{Bk\WyuIIDyc4xq\mW{YYTpc44> NVrmToVPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlc3QDhpPkK0N|Y4Pjh6PD;hQi=>
H460 M2nH[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXS1JOK2VQ>? NVGyUYpnOjRiaB?= M4TzWmROW09? M1i0[XBzd22xdHXzJINmdGxicILvcIln\XKjdHnvci=> M3zOTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{[3Olg5Lz5{NEO2O|Y5QDxxYU6=
embryonic stem cells (ESC) M{W3NWZ2dmO2aX;uJGF{e2G7 M4fKN|MhyrWP NWqxNnFyOTFiZB?= NXjMUIVJTE2VTx?= MVHQdo9ud3SnczDz[YxnNXKnbnX3ZYwhd2ZibnJDs5ZmNXS7cHWgcY92e2ViZX3idplwdmmlIIP0[Y0h[2WubIOgLGVUSyl? MmqwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NEOzNVIoRjJ|OESzN|EzRC:jPh?=
DN3 MkHGSpVv[3Srb36gRZN{[Xl? MoXrNVAh|ryP NHzYNZc6PiCq MUTEUXNQ NVfN[GtJUW6lcnXhd4V{KGOnbHyg[IV3\WyxcH3lcpQh[W6mIIDyc4xq\mW{YYTpc44> MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV{Nkm4PUc,OjN3Mk[5PFk9N2F-
platelets MnXUSpVv[3Srb36gRZN{[Xl? NFjkRVgyOCEQvF2= M1XsXlE2KG2rbh?= MWjEUXNQ NV\i[VZYUW6lcnXhd4V{KHSqcn;tZolvNW2nZHnheIVlKHCuYYTlcIV1KGGpZ4Ll[4F1cW:wIHHu[EBCXFBic3XjdoV1cW:w NX3wVWVzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyN|k5PzdpPkKzNlM6QDd5PD;hQi=>
hiPSC-NPs NUHBSXN3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jkOFPjiIoQvF2= NWL0doVQPzJiaB?= NVvVWoRJTE2VTx?= NGrMSIpKdmO{ZXHz[ZMh[2WubDDwdo9tcW[ncnH0bY9vyqB? NUe0S4lQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFI3QDdpPkKyOlQzPjh5PD;hQi=>
INS-1E NXzhPItSTnWwY4Tpc44hSXO|YYm= MXqyMlUh|ryP NX7LfGQ2OjRiaB?= MXfEUXNQ NELkTnlKdmS3Y3XzJIEhemWmdXP0bY9vKG:oIF7QNUBwfmW{ZYjwdoV{e2mxbh?= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{N{ewOEc,OjJ2Mke3NFQ9N2F-
PANC1 NU[5bmFCSXCxcITvd4l{KEG|c3H5 NXLvOZhlyqB3IN88US=> M1ezclczKGh? NUe3XGJkTE2VTx?= M1jQOXRzcWepZYLzJIFvKGGyb4D0c5Rq[yC{ZYPwc45{\Q>? NX7QZXA3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyNFEyQDZpPkKyNlAyOTh4PD;hQi=>
Endothelial cell(EC) MlX1SpVv[3Srb36gRZN{[Xl? NUL6c4hCOyEQvF2= MkXoSG1UVw>? NYLpTpF7WHKxbX;0[ZMh\W6mb4To[Yxq[WxiY3XscEApTUNrIHTp[oZmemWwdHnheIlwdg>? NXXoWGJ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwOVIxODZpPkKyNFUzODB4PD;hQi=>
HMC1.2 M4O5TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFi4e5g{OCEQvF2= MXu3NkBp Mnv6SG1UVw>? MVvS[YR2[2W|IHPlcIwhe3W{dnn2ZYw> M1juclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEO5N|AyLz5{MkCzPVMxOTxxYU6=
Sca-1+ HPC NVS0SIVXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDZNkDPxE1? MW[2JIQ> NFjNSIhFVVOR MYTTfY5memerc4TpZ4FtdHlicILvcY91\XNiU3PhMVEsyqCKUFOgdJJwdGmoZYLheIlwdiCjbnSgZ49td267IH\vdo1ifGmxbjD0c4dmfGincjD3bZRpKEWJRh?= M3TiOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNkK0N|Q5Lz5{MU[yOFM1QDxxYU6=
Oct4/Sox2/Klf4-infected MEFs MmW3SpVv[3Srb36gRZN{[Xl? NIXWZYkyOCEQvF2= MVKyPEBl M1fpTGROW09? NHzDVVhRem:vb4Tld{BOTU[|IILldJJw\3KjbX3pcochXHKjboPkeYNm\CCkeTDPZ5Q1NCCVb4iyMEBidmRiS3zmOC=> M4X2clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OEO5NFU2Lz5zOUizPVA2PTxxYU6=
H4IIE M4rreGZ2dmO2aX;uJGF{e2G7 NV62OHYzOTBizszN M4fQ[lI1KGh? NIPMeWhFVVOR NYP6UZNiWmWpdXzheIV{KM7{LXPheIVvcW5iYXP0bZZqfHliYX7kJHRKWkViY3;ueIFqdmmwZzDwdo9ud3SncjDhZ5Rqfmm2eR?= NEfQc3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUO1NFE6PSd-MUWzOVAyQTV:L3G+
insulin receptor–expressing CHO-IR M{\U[2Z2dmO2aX;uJGF{e2G7 NFTPZlk2ODBibl2= MWKzNEBucW5? M3vN[2ROW09? NYToUY5ySWO2aY\heIV{KEeueXPv[4VvKFO7boToZZNmKCiJUzm= NUjpOWI3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK2NFY1QTdpPkGyOlA3PDl5PD;hQi=>
3T3-L1 NUOzSlF2TnWwY4Tpc44hSXO|YYm= M1;HWlMh|ryP Ml7wOEBp M4Hl[GROW09? MVrJcohq[mm2czDwdoVi\Gmyb3P5eIUh\GmoZnXy[Y51cWG2aX;u MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjB3NUKwNEc,OTJyNUWyNFA9N2F-
CHO-IR NWLVc2x[TnWwY4Tpc44hSXO|YYm= M37uNlMxKG2rbh?= NGnpboZGSzVyPUCuO|ch|ryP MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTBzNkGyNUc,OjlyMU[xNlE9N2F-
HepG2 MYXGeY5kfGmxbjDBd5NigQ>? NVTET4h{OyCq NVvVe3YyTUN3ME2xMlUh|ryP NVLyW3MxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlExOjNpPkKyNlYyODJ|PD;hQi=>
Assay
Methods Test Index PMID
Western blot Cleaved PARP / CD31 / VE-Cadherin / α-SMA / vimentin ; CTNNB1 ; β-catenin 30709379 23993102 24165128
Growth inhibition assay Cell viability 22039301
Immunofluorescence SOX10 / EdU ; Oct3/4 28174705 24779365
In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. [1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. [4]

Protocol (from reference)

Kinase Assay:

[1]

  • Cell-free kinase assays:

    Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.

Cell Research:

[3]

  • Cell lines: INS-1E
  • Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM
  • Incubation Time: 1, or 4 days
  • Method:

    Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system.

Animal Research:

[1]

  • Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes
  • Dosages: ~48 mg/kg
  • Administration: Orally

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.

24mg/mL

Chemical Information

Molecular Weight 501.8
Formula

C22H18Cl2N8.HCl

CAS No. 1797989-42-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=CN=C(N1)C2=CN=C(N=C2C3=C(C=C(C=C3)Cl)Cl)NCCNC4=NC=C(C=C4)C#N.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?

Answer:
The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.

Question 2:
How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?

Answer:
Please change the culture medium each day to add the new comound in it if the duration is more than 72h.

Question 3:
What is the difference between S2924 and S1263? Which one is better for differentiation?

Answer:
S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

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