CHIR-99021 (CT99021) HCl

Catalog No.S2924

CHIR-99021 (CT99021) HCl Chemical Structure

Molecular Weight(MW): 501.8

CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.

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In DMSO USD 340 In stock
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Cited by 126 Publications

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. [1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). [2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
3T3-L1 M1nqb2Z2dmO2aX;uJGF{e2G7 MYezJO69VQ>? NHO5XG01KGh? NIO4SYtFVVOR MUPJcohq[mm2czDwdoVi\Gmyb3P5eIUh\GmoZnXy[Y51cWG2aX;u NEXVdHMyOjB3NUKwNC=>
insulin receptor–expressing CHO-IR M2XtOGZ2dmO2aX;uJGF{e2G7 NVH4c29qPTByIH7N MUezNEBucW5? MnnsSG1UVw>? MX\BZ5RqfmG2ZYOgS4x6[2:pZX6gV5lvfGijc3WgLGdUMQ>? MmHNNVI3ODZ2OUe=
H4IIE NHH1dY5HfW6ldHnvckBCe3OjeR?= MmTZNVAh|ryP MljINlQhcA>? MXXEUXNQ MXTS[Yd2dGG2ZYOg{tIu[2G2ZX7pckBi[3Srdnn0fUBidmRiVFnSSUBkd262YXnubY5oKHC{b33veIVzKGGldHn2bZR6 Mn\mNVU{PTBzOUW=
Oct4/Sox2/Klf4-infected MEFs MV3GeY5kfGmxbjDBd5NigQ>? NECybXkyOCEQvF2= MnS4Nlgh\A>? M1nuOGROW09? MY\Qdo9ud3SnczDNSWZ{KHKncILv[5JidW2rbnegWJJidnOmdXPl[EBjgSCRY4S0MEBUd3h{LDDhcoQhU2yoNB?= M{jXXFE6QDN7MEW1
Sca-1+ HPC M{nJV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXuyJO69VQ>? M4DWc|Yh\A>? Ml\qSG1UVw>? M2e3WXN6dmW{Z3nzeIlk[WyueTDwdo9ud3SnczDTZ4EuOSwEoFjQR{Bxem:uaX\ldoF1cW:wIHHu[EBkd2yxbomg[o9zdWG2aX;uJJRw\2W2aHXyJJdqfGhiRVfG NH\ve4ozOTZ{NEO0PC=>
HMC1.2 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3nIbVMxKM7:TR?= NHTmfGs4OiCq NHjIcJdFVVOR MVPS[YR2[2W|IHPlcIwhe3W{dnn2ZYw> NUDV[2xROjJyM{mzNFE>
Endothelial cell(EC) NWT6OnpKTnWwY4Tpc44hSXO|YYm= MlX3N{DPxE1? MUXEUXNQ NWLaclc1WHKxbX;0[ZMh\W6mb4To[Yxq[WxiY3XscEApTUNrIHTp[oZmemWwdHnheIlwdg>? M3[4UlIzODV{MEC2
PANC1 NYfPRldSSXCxcITvd4l{KEG|c3H5 NUHlbnViyqB3IN88US=> NYDYdXdZPzJiaB?= NVrDXGY1TE2VTx?= NIf3WoxVemmpZ3Xyd{BidiCjcH;weI91cWNicnXzdI9ve2V? M3HSWFIzOjBzMUi2
INS-1E M17RU2Z2dmO2aX;uJGF{e2G7 NIrCN2kzNjVizszN MXSyOEBp M4TGN2ROW09? MnrnTY5lfWOnczDhJJJm\HWldHnvckBw\iCQUEGgc5ZmemW6cILld5Nqd25? MnXENlI1Ojd5MES=
hiPSC-NPs MnziS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHTjOoc{6oDLzszN NH\od4s4OiCq MlmwSG1UVw>? MljpTY5kemWjc3XzJINmdGxicILvcIln\XKjdHnvcuKh NXjxRllqOjJ4NEK2PFc>
platelets MVLGeY5kfGmxbjDBd5NigQ>? NHPnZmUyOCEQvF2= MUmxOUBucW5? Ml[5SG1UVw>? NFnxR5VKdmO{ZXHz[ZMhfGi{b33ibY4udWWmaXH0[YQheGyjdHXs[ZQh[WepcnXnZZRqd25iYX7kJGFVWCC|ZXPy[ZRqd25? MU[yN|I{QTh5Nx?=
DN3 MVrGeY5kfGmxbjDBd5NigQ>? MYSxNEDPxE1? M2nNPFk3KGh? M{Lw[WROW09? MWnJcoNz\WG|ZYOgZ4VtdCCmZY\lcI9xdWWwdDDhcoQheHKxbHnm[ZJifGmxbh?= NGCwOVczOzV{Nkm4PS=>
embryonic stem cells (ESC) NXjNeYJZTnWwY4Tpc44hSXO|YYm= NFn4XZM{KML3TR?= NX3meW9jOTFiZB?= NUf4RZQ4TE2VTx?= Ml7aVJJwdW:2ZYOgd4Vt\i2{ZX7le4FtKG:oIH7hx893\S22eYDlJI1wfXOnIHXtZpJ6d26rYzDzeIVuKGOnbHzzJEhGW0Nr NFLyPIwzOzh2M{OxNi=>
H460 M2e1emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHLWUHg2KML3TR?= MkTINlQhcA>? NHjTSWxFVVOR MWrQdo9ud3SnczDj[YxtKHC{b3zp[oVz[XSrb36= NWL0PY82OjR|Nke2PFg>
H157 MlzLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnT0OUDDvU1? NG\UZY0zPCCq NVT5c|ZsTE2VTx?= NEPEdWNRem:vb4Tld{Bk\WyuIIDyc4xq\mW{YYTpc44> NIW0RWUzPDN4N{[4PC=>
H460 NX;LWHZZSXCxcITvd4l{KEG|c3H5 M2G5PFUhyrWP NUTYUJNHOjRiaB?= Mn[zSG1UVw>? NVK4ToNPSXS2ZX71ZZRmeyClZXzsJIFxd3C2b4Ppdy=> M3LZc|I1OzZ5Nki4
H157 MYDBdI9xfG:|aYOgRZN{[Xl? M1jENlUhyrWP M1fsUFI1KGh? MmPzSG1UVw>? M4fSW2Rw\XNibn;0JJNq\26rZnnjZY51dHliYX\m[YN1KGGyb4D0c5Nqew>? MXGyOFM3PzZ6OB?=
A549 NHzLR49CeG:ydH;zbZMhSXO|YYm= NXnxcYlsPSEEtV2= NV\rW2F[OjRiaB?= MY\EUXNQ NFu1XWhKdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYO= M1H3VVI1OzZ5Nki4
Neural progenitor cells M37DTmZ2dmO2aX;uJGF{e2G7 M{PEZ|Mh|ryP NWGzbJBCOTBiZB?= M{TPZmROW09? NXvjSZpDUW6mdXPld{Bo\W6ncnH0bY9vKG:oIH7leZJidCCycn;n[Y5qfG:{IHPlcIx{ NVq3Z|g5OjR4M{iwN|Q>
ES-D3 NHXVdWNEgXSxdH;4bYMhSXO|YYm= NUWxfGF7OTEEoN88US=> NGDEW2o{KGR? M{LZOWROW09? Mn;tTY5lfWOnczDtc4RmemG2ZTDjfZRwfG:6aXPpeJkhf2m2aDDJR|UxKG:oIESuPUDPxE1? M{jyOVI1Pzd7M{[1
ES-CCE MVLDfZRwfG:6aXOgRZN{[Xl? M{W3N|ExyqEQvF2= M4TCZVMh\A>? M2jMXGROW09? Mk\rTY5lfWOnczDtc4RmemG2ZTDjfZRwfG:6aXPpeJk> NIPCPWszPDd5OUO2OS=>
ES-D3 M2HLZmZ2dmO2aX;uJGF{e2G7 M17CRlXDqM7:TR?= MUizJIQ> NV3UUWk{TE2VTx?= NFn4dGlUfHKxbnfsfUBqdmS3Y3XzJJRp\SCjY4Tpeol1gSCxZjDXcpQw[mW2YT3jZZRmdmmwIIDheIh4[Xl? MWWyOFc4QTN4NR?=
ES-CCE MlPzSpVv[3Srb36gRZN{[Xl? NHfDeJo2yqEQvF2= MlzaN{Bl NID4U5pFVVOR M4XqNnN1em:wZ3z5JIlv\HWlZYOgeIhmKGGldHn2bZR6KG:oIGfueE9j\XSjLXPheIVvcW5icHH0bJdigQ>? MUCyOFc4QTN4NR?=
SW982 NIXP[ZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTBwMkG0NVYh|ryP MWLTRW5ITVJ?
GCT Mnu0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3r6N2lEPTB;MD6zNFY4OiEQvF2= MVnTRW5ITVJ?
769-P MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HRNmlEPTB;MD61PFQ6QSEQvF2= MX\TRW5ITVJ?
LOXIMVI MknCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUC0[4RKUUN3ME2wMlU5QDN{IN88US=> MX7TRW5ITVJ?
D-336MG M1rkSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVPrUGRxUUN3ME2wMlg1PjJizszN NYraclFKW0GQR1XS
S-117 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PnUGlEPTB;MD65NlE{OSEQvF2= MmXFV2FPT0WU
D-247MG NHjWSo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUPsdmYxUUN3ME2wMlk1QDV{IN88US=> MlLxV2FPT0WU
TYK-nu Mm\PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2[zN2lEPTB;MT6wO|kyOiEQvF2= MlXPV2FPT0WU
A498 M3W5SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTFwM{C0Olkh|ryP MXHTRW5ITVJ?
BCPAP NYTlO|FCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm[4TWM2OD1zLk[2PFk5KM7:TR?= M4nCVHNCVkeHUh?=
KYSE-180 NYTIfIY{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTFwN{K3PFgh|ryP M3LkOHNCVkeHUh?=
MIA-PaCa-2 NFmycZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnz0TWM2OD1zLke1PVc4KM7:TR?= MkG0V2FPT0WU
HCC1395 M3qyR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NW\UXmFCUUN3ME2xMlc5Ojd|IN88US=> NIXibHpUSU6JRWK=
RS4-11 NITndItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVeyOmlyUUN3ME2xMlk2PDF3IN88US=> MV\TRW5ITVJ?
NCI-H2122 NULxb5pNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkXtTWM2OD1{LkK3Olg1KM7:TR?= M{W1[HNCVkeHUh?=
SNU-423 NG\EcYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkCyTWM2OD1{LkS5O|g4KM7:TR?= MULTRW5ITVJ?
SK-LMS-1 M4j4XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLaeYY6UUN3ME2yMlY1OTJ6IN88US=> NFrlNnVUSU6JRWK=
RPMI-7951 MlXiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3zHWGlEPTB;Mj63NFE6OyEQvF2= NFH4PZZUSU6JRWK=
COLO-829 M4jmd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXDRmFTUUN3ME2yMlc3PTRizszN NV\5VI8xW0GQR1XS
SCC-9 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXfJR|UxRTJwOUW0PFUh|ryP MVnTRW5ITVJ?
A704 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nm[2lEPTB;Mz6wPFEzKM7:TR?= M2XsZXNCVkeHUh?=
HOP-62 NFzKdG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTNwM{[wOVQh|ryP NWTlcoVZW0GQR1XS
HCC1569 NXroUpA4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEHYeGdKSzVyPUOuOFg{ODVizszN MYPTRW5ITVJ?
A172 NHjrdJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTNwN{W0PVgh|ryP NVTM[Gd{W0GQR1XS
MOLT-16 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHjTWM2OD1|Lki4OVU3KM7:TR?= M3jkU3NCVkeHUh?=
TE-15 NHnVSnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfzTWM2OD1|Lkm1N|Q{KM7:TR?= MoHvV2FPT0WU
OE19 MnqxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HtfmlEPTB;Mz65OlQ2OSEQvF2= NWKwOVFsW0GQR1XS
MHH-ES-1 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHQN5JkUUN3ME20MlA1PTR3IN88US=> MWHTRW5ITVJ?
NKM-1 MkLIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkH5TWM2OD12LkGzPVcyKM7:TR?= MY\TRW5ITVJ?
RCC10RGB MoLhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkfwTWM2OD12LkK5OFc6KM7:TR?= NWDMVYxxW0GQR1XS
BxPC-3 NIPYTFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\oTWM2OD12LkewN|kyKM7:TR?= M2PubHNCVkeHUh?=
ALL-PO MlvXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUXnT5VKUUN3ME20Mlg{QDV6IN88US=> NXLzToRiW0GQR1XS
SK-OV-3 NUSwW4dGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkiyTWM2OD12Lkm5N|Qh|ryP MWjTRW5ITVJ?
SW1710 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXe1ZlI2UUN3ME21MlQzPDV4IN88US=> NIXQNGhUSU6JRWK=
NCI-H2452 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MluyTWM2OD13LkS1OlI5KM7:TR?= NU\1c|ZmW0GQR1XS
D-392MG MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkT1TWM2OD13LkS5NVQ2KM7:TR?= NGjKUXRUSU6JRWK=
LB2518-MEL MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRTVwN{K2Nlch|ryP NVLCZ3h{W0GQR1XS
BL-41 NG\ncpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn[4TWM2OD13LkixNlY3KM7:TR?= Mni5V2FPT0WU
WM-115 MkjKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLZTWM2OD13LkixOVA6KM7:TR?= M3TGPHNCVkeHUh?=
SK-MEL-2 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;rTWM2OD14LkO4N|Q1KM7:TR?= NHzLToJUSU6JRWK=
CGTH-W-1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUS5N|JtUUN3ME22MlQyPyEQvF2= NEXkPXpUSU6JRWK=
BB65-RCC M1HyU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLhS5V5UUN3ME22MlU{OTl3IN88US=> MXvTRW5ITVJ?
CAL-85-1 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjaTWM2OD14Lk[wPVM3KM7:TR?= NUGwcmlPW0GQR1XS
RT-112 NY\yPZo4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnwVnpKUUN3ME23MlE3PDV4IN88US=> M1rxW3NCVkeHUh?=
MC116 NX21e5FXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvj[XZmUUN3ME23MlI5PjlzIN88US=> NHv5ZnZUSU6JRWK=
G-361 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TFcmlEPTB;Nz60OlU3OSEQvF2= NVjwXmhSW0GQR1XS
SW1417 NUThNW83T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PyS2lEPTB;Nz62OFU5PCEQvF2= MX;TRW5ITVJ?
MV-4-11 MoPQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRThwNUSzNFQh|ryP M3vIeXNCVkeHUh?=
CAL-72 NImxWWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoHoTWM2OD16Lke2NVE{KM7:TR?= MXvTRW5ITVJ?
MKN28 NXu4cWNNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\Wd4NKSzVyPUiuPFg2OTZizszN NVLiVlJtW0GQR1XS
MEG-01 NVTMZnVHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRThwOUmwOFMh|ryP M1XLb3NCVkeHUh?=
ACHN NV;mS|BPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLlTWM2OD17LkOwOVQ3KM7:TR?= M4fzWXNCVkeHUh?=
NCI-H1755 NGnw[21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX\3U|VsUUN3ME25MlM6QTh4IN88US=> NWTReJBvW0GQR1XS
NCI-H64 NVjwXpdlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTlwNEKyOVUh|ryP MkXoV2FPT0WU
COLO-205 NWLQOo1mT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2HkRWlEPTB;OT60N|gzOSEQvF2= M3;uNXNCVkeHUh?=
CTV-1 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHrLb2ZKSzVyPUmuOFM5QTRizszN NIWy[ZRUSU6JRWK=
U-2-OS MnrrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXL1VmF2UUN3ME25MlQ2ODB4IN88US=> MYjTRW5ITVJ?
LB996-RCC NIfBZYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4LoSGlEPTB;OT61NlM6PyEQvF2= MVHTRW5ITVJ?
SK-N-DZ NFj3VppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\3dWlEPTB;OT62OVc1OiEQvF2= M1\PbXNCVkeHUh?=
EFM-19 NFro[HpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1XjdWlEPTB;OT63OVU3PiEQvF2= NED1SolUSU6JRWK=
DBTRG-05MG Mn7FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXTJR|UxRTlwN{e3O{DPxE1? M1ftNXNCVkeHUh?=
U-118-MG MmP1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjkOXBKSzVyPUmuPVQ1PDRizszN NXjsNVdJW0GQR1XS
LB1047-RCC NIXzSY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NITsPHhKSzVyPUmuPVk4QThizszN MkDOV2FPT0WU
NCI-H28 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLwXJFKSzVyPUGwMlQ1OTNizszN MmLHV2FPT0WU
MZ2-MEL NHzGdWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\oXGlEPTB;MUCuOFY4PyEQvF2= NWjGUYRUW0GQR1XS
M059J NHfSNIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTFyLk[xNFIh|ryP NUe1UZpvW0GQR1XS
HT-3 M2e5WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{PUe2lEPTB;MUCuOlE4QSEQvF2= NIDOXWlUSU6JRWK=
SNU-387 M3rqSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYfJR|UxRTFyLkeyN|Uh|ryP MVvTRW5ITVJ?
NCI-H526 M375WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGqyT3hKSzVyPUGxMlE{PjVizszN Mme0V2FPT0WU
HEL NFPPRlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPTTWM2OD1zMT62OVc6KM7:TR?= MlnhV2FPT0WU
PA-1 NGDM[YpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DwbmlEPTB;MUGuPVMh|ryP MofDV2FPT0WU
PC-14 MoPlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3PTWM2OD1zMj6zNlQh|ryP MmjZV2FPT0WU
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SBC-1 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLDc2lPUUN3ME24OE4xOjB6IN88US=> MYPTRW5ITVJ?
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TE-12 MnfPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUT1Tm51UUN3ME24PU4{PzRzIN88US=> MmnCV2FPT0WU
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CAL-33 NH3tWI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzkTWM2OD17Mz6wOlAyKM7:TR?= MYDTRW5ITVJ?
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IGR-1 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTl7LkOxNVkh|ryP M3HxOHNCVkeHUh?=
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HOP-92 M17mdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7xSlJxUUN3ME2xNFgvPDd3IN88US=> M1X2U3NCVkeHUh?=
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DU-145 NEXYXpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NULUZXBuUUN3ME2xNFkvQTR3IN88US=> M3PqVnNCVkeHUh?=
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WSU-NHL MkjyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkXmTWM2OD1zMUKuNFEh|ryP NITzc2lUSU6JRWK=
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SIG-M5 M2ruWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkLJTWM2OD1zMUOuNVI2KM7:TR?= NFzsUZJUSU6JRWK=
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OVCAR-8 NGXlV2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWLtdYdsUUN3ME2xNVcvPjZ6IN88US=> M4Gxc3NCVkeHUh?=
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OVCAR-3 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4KzWGlEPTB;MUG5MlkyPSEQvF2= MorDV2FPT0WU
NCI-N87 NXPwS41pT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYXxV3I{UUN3ME2xNlEvODR2IN88US=> NXz0N2NGW0GQR1XS
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KYSE-140 MmPhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;OTWM2OD1zMkKuNVQyKM7:TR?= NH7DO3lUSU6JRWK=
HAL-01 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXTfGZRUUN3ME2xNlIvPDl7IN88US=> NGLvWFhUSU6JRWK=
HT-29 M1zpRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MofqTWM2OD1zMkKuO|c5KM7:TR?= MW\TRW5ITVJ?
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SW1463 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTF{ND6wO|ch|ryP M1HDXnNCVkeHUh?=
NCI-H2087 MkPMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1;i[2lEPTB;MUK0MlI5OyEQvF2= M3jNTXNCVkeHUh?=
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EB-3 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXvYXmE{UUN3ME2xNlcvODF|IN88US=> Mlv5V2FPT0WU
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LU-99A MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{Di[GlEPTB;MUK5Mlk{OSEQvF2= MWLTRW5ITVJ?
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HT-1080 M4n6b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rNTGlEPTB;MUOyMlIxPCEQvF2= MnnkV2FPT0WU
CTB-1 NFKzcpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVnXe|c2UUN3ME2xN|IvQDl5IN88US=> NGTiW2pUSU6JRWK=
Capan-2 NFX2fpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVexTWN[UUN3ME2xN|MvQTd3IN88US=> MlTuV2FPT0WU
BB30-HNC NF\2bpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHp[WNOUUN3ME2xN|QvOTF4IN88US=> NYnjWWtZW0GQR1XS
CP50-MEL-B M4O0[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnHRTWM2OD1zM{SuNkDPxE1? NVHUR5ZXW0GQR1XS
JEG-3 NVzVZpZuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHrGbYlKSzVyPUGzOE4{PTlizszN MnHwV2FPT0WU
NCI-H1437 Ml[0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LCOWlEPTB;MUO0MlU6KM7:TR?= NUe3ZZZ7W0GQR1XS
NOS-1 Mm\JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7xUWVJUUN3ME2xN|QvPzZ3IN88US=> M2rxNXNCVkeHUh?=
G-402 NHXvNIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDuOoY2UUN3ME2xN|UvOjl3IN88US=> NWHMeZg1W0GQR1XS
OAW-42 NHzzXIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MofJTWM2OD1zM{euPFU3KM7:TR?= NVnRUHQyW0GQR1XS
Ca-Ski MoTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXjJR|UxRTF|OD64N|Eh|ryP NV20NXNkW0GQR1XS
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OVCAR-4 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTF2MD63OFkh|ryP MVzTRW5ITVJ?
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NCI-H2052 NFHye|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjLZmhjUUN3ME2xOVkvPTh|IN88US=> MlnzV2FPT0WU
EFO-27 MkjLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUX2SmZSUUN3ME2xOlAvPTh2IN88US=> NXzSVmI1W0GQR1XS
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JVM-2 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nUOGlEPTB;MU[3MlQ2OSEQvF2= NH[2U2hUSU6JRWK=
OVCAR-5 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXyTWM2OD1zNkiuN|A6KM7:TR?= MXLTRW5ITVJ?
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COLO-741 MnL1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37GdmlEPTB;MUe3MlU3OSEQvF2= M4n4bnNCVkeHUh?=
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ONS-76 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NILQ[pZKSzVyPUG4Nk4{QDdizszN MoPmV2FPT0WU
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LB2241-RCC NHHx[pNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1PBZWlEPTB;MUi1MlE1PSEQvF2= MmDjV2FPT0WU
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CAPAN-1 MoHJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1;lTWlEPTB;MUi3MlQ2OyEQvF2= NUjjdIZyW0GQR1XS
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COLO-668 NF74UGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjmTWM2OD1zOUOuNVU4KM7:TR?= NHrZPXlUSU6JRWK=
HGC-27 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnBZZZbUUN3ME2xPVUvPTN6IN88US=> MmTMV2FPT0WU
SW1088 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXXPT2htUUN3ME2xPVUvPjd5IN88US=> NUfjPI1xW0GQR1XS
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NCI-H345 M4XFRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoW3TWM2OD1{MEOuNFI4KM7:TR?= MYDTRW5ITVJ?
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NUGC-3 MljTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWC3[oN{UUN3ME2yNFkvPjd6IN88US=> NFf5XYpUSU6JRWK=
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EW-18 M1rNZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{K1bmlEPTB;MkGwMlc3OyEQvF2= NUO4d5RRW0GQR1XS
BT-20 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjSTWM2OD1{MUCuPVg3KM7:TR?= NHm2VYFUSU6JRWK=
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TCCSUP M4i0PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;PfHVtUUN3ME2yNVEvPTN|IN88US=> M1XuN3NCVkeHUh?=
HO-1-N-1 MmjkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnGT4ltUUN3ME2yNVIvQDl2IN88US=> MVXTRW5ITVJ?
NCI-H69 M1m2cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXPTWM2OD1{MUSuNVg2KM7:TR?= MkK1V2FPT0WU
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NB13 NV;sRXMyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvQRW9KSzVyPUO0OU42QCEQvF2= NGTTR29USU6JRWK=
D-502MG MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TqTGlEPTB;M{S1MlgyOiEQvF2= NEL5XopUSU6JRWK=
CAL-54 NF;WOWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrheWRKSzVyPUO0OU45PzlizszN NHHpUYlUSU6JRWK=
DOHH-2 M3SzWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnrjTWM2OD1|NE[uOlUh|ryP MlnsV2FPT0WU
SK-PN-DW MkXKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGnQXZNKSzVyPUO0O{41OjZizszN NE[3d49USU6JRWK=
NCI-H720 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7sW4JNUUN3ME2zOFkvQDR4IN88US=> NUe3flFkW0GQR1XS
SW1990 M4jrOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzleFFKSzVyPUO1NU4zQCEQvF2= MV3TRW5ITVJ?
NCI-H2196 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlG5TWM2OD1|NUKuOlI1KM7:TR?= MWnTRW5ITVJ?
P30-OHK NWT4UVJUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jwemlEPTB;M{WzMlA3OyEQvF2= MUHTRW5ITVJ?
NCI-H358 NG\lSoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;3cW02UUN3ME2zOVUvOzV4IN88US=> MXfTRW5ITVJ?
CW-2 Mlr2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nNdmlEPTB;M{W2MlUzQSEQvF2= NHSwSJpUSU6JRWK=
COLO-678 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zj[GlEPTB;M{W3Mlg3OyEQvF2= MWPTRW5ITVJ?
NCI-H2126 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoriTWM2OD1|NUiuOVIyKM7:TR?= Mk\KV2FPT0WU
DMS-53 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLpTWM2OD1|NUiuO|EyKM7:TR?= MoDUV2FPT0WU
NCI-H1838 M1;YfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4P1[mlEPTB;M{W5Mlk5PyEQvF2= NV3mTlFZW0GQR1XS
SJSA-1 M1T0cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFPPcnRKSzVyPUO2NE46OzlizszN MUTTRW5ITVJ?
ChaGo-K-1 NGixXolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TtTmlEPTB;M{[zMlkzPCEQvF2= MlHrV2FPT0WU
IST-SL2 NIHZeWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTN5Mj65NlYh|ryP M2XMNXNCVkeHUh?=
U-266 NEP3XIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn30TWM2OD1|N{OuNVMzKM7:TR?= MYfTRW5ITVJ?
P31-FUJ M{jUWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXPJR|UxRTN5Nj65OlQh|ryP NF;NOm5USU6JRWK=
HCC2157 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vKUGlEPTB;M{e4MlE3OSEQvF2= NXfSVpdZW0GQR1XS
COR-L88 MnPSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvXXoZKSzVyPUO4N{43PzdizszN NIqxfo5USU6JRWK=
NCI-H596 MkTDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3;vNmlEPTB;M{i1MlM1KM7:TR?= M1P2PXNCVkeHUh?=
GOTO NHPNVWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\xTVJKSzVyPUO5NE43PDFizszN M{DwdXNCVkeHUh?=
NCI-H719 Mk\5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;BTWM2OD1|OUGuPFk{KM7:TR?= MmLBV2FPT0WU
MN-60 NF7FSpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI\ZbmdKSzVyPUO5N{46PDhizszN NUe5b4JtW0GQR1XS
IST-SL1 NITPflRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlnKTWM2OD1|OUSuOFA3KM7:TR?= MV;TRW5ITVJ?
TE-6 NIntPIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rtXmlEPTB;M{m3MlI2PCEQvF2= MULTRW5ITVJ?
SW962 M3nTT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;lTWM2OD1|OUiuOlkh|ryP M3PWS3NCVkeHUh?=
FADU MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{T2e2lEPTB;NECzMlA5OyEQvF2= MXrTRW5ITVJ?
ECC4 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\n[2lEPTB;NEC4MlQ2QCEQvF2= M37rXHNCVkeHUh?=
MES-SA NWHLVG1QT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXq1TI1XUUN3ME20NFkvQThizszN MVvTRW5ITVJ?
NCI-H716 MnqyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRTRzMD6wO|Uh|ryP MYXTRW5ITVJ?
GR-ST M4nmdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHWXWNKSzVyPUSxNE41QTdizszN NIn1SlZUSU6JRWK=
KY821 NFPtOFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVrJR|UxRTRzND6wPFYh|ryP Mk\SV2FPT0WU
NCI-H2141 MorGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX\rNng3UUN3ME20NlQvOzh6IN88US=> MYDTRW5ITVJ?
MDA-MB-134-VI NGTUdoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7EeW9UUUN3ME20N|EvODN|IN88US=> MkTkV2FPT0WU
RH-1 M17afGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHflOGtKSzVyPUSzOU4xQSEQvF2= MoTaV2FPT0WU
AM-38 Mor4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jxcmlEPTB;NEO4MlU1KM7:TR?= MVHTRW5ITVJ?
BL-70 NGf0[m9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRTR|OT60Nlkh|ryP M4HHPHNCVkeHUh?=
COLO-684 NHfqRY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInpcYxKSzVyPUS0PE45QDhizszN M4PwcXNCVkeHUh?=
DMS-79 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\TTWM2OD12NEmuOlgh|ryP NFm3bGNUSU6JRWK=
EW-1 M4jsWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnXxTWM2OD12NUKuPFg5KM7:TR?= NYLNOGx5W0GQR1XS
MS-1 NV;oZm5jT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnkT4JKSzVyPUS1OE4{QDVizszN MnHqV2FPT0WU
A4-Fuk MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTR3ND61NFch|ryP MWXTRW5ITVJ?
TALL-1 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmGwTWM2OD12NUWuNlk4KM7:TR?= NYK3SY54W0GQR1XS
SCLC-21H M4\L[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\BfHFKSzVyPUS1OU43QTlizszN MkfFV2FPT0WU
KMS-12-PE MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFjuN3ZKSzVyPUS1Ok45OjJizszN M3X3PHNCVkeHUh?=
TE-441-T M13MUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWCydFhiUUN3ME20OVcvOTh4IN88US=> NHu1SWxUSU6JRWK=
A3-KAW MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkmxTWM2OD12NkCuPFQyKM7:TR?= NUW0ZXRwW0GQR1XS
NCI-H1092 M2\kXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTR4Mz65OFMh|ryP NWfvWIVIW0GQR1XS
COR-L279 NWKwbnM6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTR4ND64OFch|ryP M3roSHNCVkeHUh?=
NCI-SNU-5 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHqWGlKSzVyPUS3Nk4zQCEQvF2= MYjTRW5ITVJ?
JAR NYfWfGRLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVK3SWxjUUN3ME20O|QvPzh6IN88US=> MVfTRW5ITVJ?
NCI-H446 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVzzbWF2UUN3ME20O|cvODhizszN MVjTRW5ITVJ?
HDLM-2 M{D1emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUX1[nRyUUN3ME20PFAvQDZ7IN88US=> M4LYUnNCVkeHUh?=
D-283MED Ml;ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\ySZNHUUN3ME20PFgvQTJ2IN88US=> MVnTRW5ITVJ?
NCI-H1395 M{L1XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MonBTWM2OD12OEmuNFA1KM7:TR?= MWjTRW5ITVJ?
RPMI-6666 Mle0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\4N|JxUUN3ME20PVEvOzl6IN88US=> MYPTRW5ITVJ?
CPC-N MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fLcGlEPTB;NUCyMlA3OyEQvF2= NX;Xc212W0GQR1XS
EC-GI-10 M4O3S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mor3TWM2OD13MEKuNVY6KM7:TR?= Mne3V2FPT0WU
CA46 NGr1[WxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fRdmlEPTB;NUC0MlQxPyEQvF2= NXTN[o9kW0GQR1XS
EHEB MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFu0OFVKSzVyPUWwO{4zODhizszN NVPVR45pW0GQR1XS
RPMI-8402 MnXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXL1bYJ6UUN3ME21NVAvODJzIN88US=> MlG2V2FPT0WU
DU-4475 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXOxUItCUUN3ME21NVEvOTBzIN88US=> MUPTRW5ITVJ?
P12-ICHIKAWA MkTXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXx[|hbUUN3ME21NVEvPDF4IN88US=> Mk\rV2FPT0WU
NCI-H1694 NGn4d5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jIOGlEPTB;NUGyMlQ{QSEQvF2= M3Xjb3NCVkeHUh?=
NCI-H2171 NIrXUpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTVzMj63NFUh|ryP M3jOPXNCVkeHUh?=
Mo-T MnnGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTVzNT6xNVYh|ryP NFPrVJZUSU6JRWK=
HL-60 MmLYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXTJR|UxRTV{Mj65NlQh|ryP MmLBV2FPT0WU
NCI-H1436 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7Wc4lwUUN3ME21NlMvODZ2IN88US=> MVLTRW5ITVJ?
NCI-H510A MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEjrWG5KSzVyPUWyOE43QTZizszN MWTTRW5ITVJ?
NCI-H2081 NXy0bYlMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTV{NT61NVMh|ryP MYHTRW5ITVJ?
NB5 M1zTemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFPFUYpKSzVyPUWyOk41OyEQvF2= NF\tRZBUSU6JRWK=
NCI-H2227 Ml:3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHW0R|FKSzVyPUWzNE4zPTRizszN NYKyUWp3W0GQR1XS
HSC-4 NVu4OIR3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkXRTWM2OD13M{muNlk{KM7:TR?= MXXTRW5ITVJ?
RL NXTVd4xxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnPQTWM2OD13NEKuOFQzKM7:TR?= MXTTRW5ITVJ?
EVSA-T NIm2TFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTV2Mz62PFQh|ryP M33OdnNCVkeHUh?=
NCI-H889 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{DOT2lEPTB;NUS3MlI{KM7:TR?= MmjBV2FPT0WU
COLO-320-HSR MmXhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HVRWlEPTB;NUWxMlM2KM7:TR?= NFfDcYhUSU6JRWK=
EW-11 NV7JSlFOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWnJR|UxRTV3Mj6zPVkh|ryP MmK0V2FPT0WU
UACC-812 NVy5RmtDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEHZVnNKSzVyPUW1PU4{QTFizszN M4nNUHNCVkeHUh?=
CAL-148 NWfWU5pLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTV4NT6wPFkh|ryP M3fmRXNCVkeHUh?=
K052 MoHrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUXsbG01UUN3ME21O|kvOjF|IN88US=> MnuxV2FPT0WU
L-428 MnPVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2DFe2lEPTB;NkCwMlY4OSEQvF2= NV3OT|I{W0GQR1XS
LP-1 M1rHeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\SXGlEPTB;NkCzMlU5PyEQvF2= M1PSOnNCVkeHUh?=
ML-2 MnjTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFTj[JJKSzVyPU[wOk42PzlizszN MYnTRW5ITVJ?
KARPAS-45 M3;keGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml[3TWM2OD14MUiuNlIyKM7:TR?= MorrV2FPT0WU
HT NGTacHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvuTWM2OD14MkKuO|g2KM7:TR?= MlG5V2FPT0WU
NCI-H226 NYPXOmhPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jxWWlEPTB;NkK4MlM2OyEQvF2= MWPTRW5ITVJ?
L-363 NFLQfIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIrDR4RKSzVyPU[0PE4xPjRizszN NYjz[4NrW0GQR1XS
IM-9 M3W1bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFf2dmpKSzVyPU[1NU42PDFizszN MYrTRW5ITVJ?
TUR NWHN[W9TT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFXZTGRKSzVyPU[3NU4{OjJizszN M4D5SHNCVkeHUh?=
NCI-SNU-16 M2LHUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTmZpdVUUN3ME22PFAvPDZ{IN88US=> MV\TRW5ITVJ?
NCI-SNU-1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEeybYlKSzVyPU[5NE4yPzJizszN Mn\OV2FPT0WU
U-87-MG Ml\VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPUNlhKSzVyPU[5OE42QDVizszN M3vv[XNCVkeHUh?=
KM-H2 NIHSbm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|UxRTZ7OT6wOVkh|ryP MWTTRW5ITVJ?
DEL MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvudHc2UUN3ME23NFAvQDN6IN88US=> MnL0V2FPT0WU
KARPAS-422 M3LQfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1fQfWlEPTB;N{i5MlMyPSEQvF2= MXXTRW5ITVJ?
BC-3 Mnr4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlG5TWM2OD16MEWuNlA{KM7:TR?= NGPKSo9USU6JRWK=
NCI-H1155 M1nW[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{X4OmlEPTB;OEG4MlA2QSEQvF2= M1zLVXNCVkeHUh?=
NCI-H1581 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmrFTWM2OD17MECuNlMyKM7:TR?= MWTTRW5ITVJ?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cleaved PARP / CD31 / VE-Cadherin / α-SMA / vimentin; 

PubMed: 30709379     


NCI-H460 cells were co-cultured with HUVECs in a 3D culture system, and then treated with 1 μM CHIR-99021, 10 μM or 20 μM Cisplatin, or a combination of both, for 24 h. Cells were harvested and immunoblotted with anti-cleaved PARP, anti-CD31, anti-VE-cadherin, anti-α-SMA, anti-vimentin, and anti-β-actin antibodies. 

CTNNB1; 

PubMed: 23993102     


Treatment with the GSK3β inhibitor CHIR-99021 led to increased levels of β-catenin in RKO and HT29 (non-mutant) cells.

β-catenin; 

PubMed: 24165128     


HeLa cells were treated with 5 μm CHIR-99021 for the indicated times. The samples were analyzed by Western blot.

30709379 23993102 24165128
Growth inhibition assay
Cell viability; 

PubMed: 22039301     


HMC-1.2 cells were cultured in the absence or presence of GSK3β inhibitor (CHIR 99021, 3–30 µM) and viability cell count was assessed by trypan blue dye exclusion after 24 h, 48 h or 72 h. All data are presented as the mean ± SEM of three independent experiments conducted in triplicate. p < 0.05*, p < 0.001** p < 0.0001*** vs vehicle treated control cells.

22039301
Immunofluorescence
SOX10 / EdU; 

PubMed: 28174705     


Incorporation of EdU (white arrowheads) into SOX10+ ENS (enteric nervous system) cells with and without CHIR-99021.

Oct3/4; 

PubMed: 24779365     


Fluorescence micrographs showing the protein expression of Brachyury/Oct3/4 after a three day differentiation of ES-D3 cells with BIO (0.5 μM), SB-216763 (5 μM), CHIR-99021 (5 μM), or CHIR-98014 (1 μM). Nuclei were counterstained with DAPI (blue). The merge is shown in yellow. Original magnification 400x. Scale bar = 50 μm.

28174705 24779365
In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. [1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. [4]

Protocol

Kinase Assay:

[1]

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Cell-free kinase assays:

Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research:

[3]

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  • Cell lines: INS-1E
  • Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM
  • Incubation Time: 1, or 4 days
  • Method:

    Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes
  • Formulation: Formulated as solutions in 20 mM citrate-buffered 15% Captisol or as fine suspensions in 0.5% carboxymethylcellulose.
  • Dosages: ~48 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (185.33 mM)
Ethanol 2 mg/mL (3.98 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
24mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 501.8
Formula

C22H18Cl2N8.HCl

CAS No. 1797989-42-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?

  • Answer:

    The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.

  • Question 2:

    How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?

  • Answer:

    Please change the culture medium each day to add the new comound in it if the duration is more than 72h.

  • Question 3:

    What is the difference between S2924 and S1263? Which one is better for differentiation?

  • Answer:

    S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID