CHIR-99021 (CT99021) HCl

For research use only.

Catalog No.S2924

337 publications

CHIR-99021 (CT99021) HCl Chemical Structure

CAS No. 1797989-42-4

CHIR-99021 (CT99021) HCl is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2. CHIR-99021 is a potent pharmacological activators of the Wnt/beta-catenin signaling pathway. CHIR-99021 significantly rescues light-induced autophagy and augments GR, RORα and autophagy-related proteins.

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Selleck's CHIR-99021 (CT99021) HCl has been cited by 337 publications

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description CHIR-99021 (CT99021) HCl is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2. CHIR-99021 is a potent pharmacological activators of the Wnt/beta-catenin signaling pathway. CHIR-99021 significantly rescues light-induced autophagy and augments GR, RORα and autophagy-related proteins.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. [1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). [2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H1581 M3TsW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTlyMD6yN|Eh|ryP MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
NCI-H1155 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vtOGlEPTB;OEG4MlA2QSEQvF2= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
BC-3 NXH5O41wT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\ofnhKSzVyPUiwOU4zODNizszN NXS2dHhpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
KARPAS-422 NV[yZ5NMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnFdo9KSzVyPUe4PU4{OTVizszN MoL6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
DEL Mke2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\XWJZKSzVyPUewNE45OzhizszN NGj2V2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
KM-H2 M1f6eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7CTWM2OD14OUmuNFU6KM7:TR?= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
U-87-MG NGG2b4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1HNcWlEPTB;Nkm0MlU5PSEQvF2= M4nne|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
NCI-SNU-1 NH70XI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTZ7MD6xO|Ih|ryP MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
NCI-SNU-16 NV\4cG1wT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnXNTWM2OD14OECuOFYzKM7:TR?= M2n3WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
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L-363 Mk\ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGDmN25KSzVyPU[0PE4xPjRizszN M3K3elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
NCI-H226 M{Ppemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1zQfmlEPTB;NkK4MlM2OyEQvF2= NEPselE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
HT MknmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPQ[4JKSzVyPU[yNk44QDVizszN MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
KARPAS-45 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEjGR3dKSzVyPU[xPE4zOjFizszN NYrGcWI1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
ML-2 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHz2PGZKSzVyPU[wOk42PzlizszN NUHuS5ZwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
LP-1 NInDc4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFfkO|hKSzVyPU[wN{42QDdizszN NVTPfllRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
L-428 NVW0W241T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk[xTWM2OD14MECuOlcyKM7:TR?= NYDYeYR3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
K052 NITOO4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV3tOG1YUUN3ME21O|kvOjF|IN88US=> MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
CAL-148 NFXPT|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWTJR|UxRTV4NT6wPFkh|ryP NX2xWW1CRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
UACC-812 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFH5OXhKSzVyPUW1PU4{QTFizszN NUm5O|c4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
EW-11 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLYTWM2OD13NUKuN|k6KM7:TR?= M3;ab|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
COLO-320-HSR MojsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFLtXFBKSzVyPUW1NU4{PSEQvF2= M1vJXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
NCI-H889 NU\OWW9vT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX[yZo91UUN3ME21OFcvOjNizszN M33IVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
EVSA-T MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTV2Mz62PFQh|ryP NUm4[mdZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
RL MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;Hb3BtUUN3ME21OFIvPDR{IN88US=> NYPxdo5zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
HSC-4 NGf6d3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU[0XFRCUUN3ME21N|kvOjl|IN88US=> M{HnWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
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NCI-H2081 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrhRpFIUUN3ME21NlUvPTF|IN88US=> MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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HAL-01 NEPWc5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTF{Mj60PVkh|ryP M37HRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
KYSE-140 MlnDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXTJR|UxRTF{Mj6xOFEh|ryP NFL0WpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
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NCI-H1755 NYLMfWJvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkHKTWM2OD17LkO5PVg3KM7:TR?= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
ACHN NVnHWmFYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGfPSYFKSzVyPUmuN|A2PDZizszN MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
MEG-01 NXHRW2gxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvWUFRoUUN3ME24Mlk6ODR|IN88US=> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
MKN28 Ml;xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\ZU2JKSzVyPUiuPFg2OTZizszN NUHJU|dHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
CAL-72 MoHXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRThwN{[xNVMh|ryP Mlm2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MV-4-11 M3ryOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGXDWZVKSzVyPUiuOVQ{ODRizszN M37nSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SW1417 NYHiUIN3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonQTWM2OD15Lk[0OVg1KM7:TR?= MoXHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
G-361 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUCwO4dUUUN3ME23MlQ3PTZzIN88US=> NWPsUZo2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
MC116 MofVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUPBb|dLUUN3ME23MlI5PjlzIN88US=> M{PpNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
RT-112 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnzxTWM2OD15LkG2OFU3KM7:TR?= NGH3XXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
CAL-85-1 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGexWnlKSzVyPU[uOlA6OzZizszN M1fRRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
BB65-RCC NV3FOolrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF3EXG1KSzVyPU[uOVMyQTVizszN MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
CGTH-W-1 NHnU[WpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\ZTWM2OD14LkSxO{DPxE1? M3vm[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
WM-115 NHPhZo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWrJR|UxRTVwOEG1NFkh|ryP MmjBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
SK-MEL-2 NHHOSo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHYTWM2OD14LkO4N|Q1KM7:TR?= NGXGXZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
BL-41 M2XESWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUG5NoI3UUN3ME21MlgyOjZ4IN88US=> M4\xZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
LB2518-MEL NYnDWGZjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LQcWlEPTB;NT63NlYzPyEQvF2= NGDaXZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
D-392MG M{HtfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfLUlI2UUN3ME21MlQ6OTR3IN88US=> Ml7PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
NCI-H2452 Mm\NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoH0TWM2OD13LkS1OlI5KM7:TR?= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
SW1710 M4O4RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXPJR|UxRTVwNEK0OVYh|ryP M{nDbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SK-OV-3 M4nSXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4PUOmlEPTB;ND65PVM1KM7:TR?= M4LrOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
ALL-PO MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1jJR2lEPTB;ND64N|g2QCEQvF2= MlfkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
BxPC-3 Ml\RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTRwN{CzPVEh|ryP MmfhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
RCC10RGB MmC0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjGUJVKSzVyPUSuNlk1PzlizszN NFPlR4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
NKM-1 NGLWO45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXjJR|UxRTRwMUO5O|Eh|ryP MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
MHH-ES-1 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWHJR|UxRTRwMES1OFUh|ryP MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
OE19 MkXmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUPXd5hiUUN3ME2zMlk3PDVzIN88US=> MmT4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
TE-15 MnntS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnUSotjUUN3ME2zMlk2OzR|IN88US=> MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
MOLT-16 MkjiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHe1N4hKSzVyPUOuPFg2PTZizszN MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
A172 NUjRdXZYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XRPGlEPTB;Mz63OVQ6QCEQvF2= NXPmUIhuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
HCC1569 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVT2SW9xUUN3ME2zMlQ5OzB3IN88US=> NHzycVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
HOP-62 NXjKNnFvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlfnTWM2OD1|LkO2NFU1KM7:TR?= NEfK[3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
A704 NXr4clFwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX\JR|UxRTNwMEixNkDPxE1? NX:yOJFyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
SCC-9 MlL4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWD5N3A2UUN3ME2yMlk2PDh3IN88US=> Mlm1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
COLO-829 NF7pNZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkLaTWM2OD1{Lke2OVQh|ryP NHG3NlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
RPMI-7951 MnvLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTJwN{CxPVMh|ryP MkLrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
SK-LMS-1 NELkNpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWDW[VZiUUN3ME2yMlY1OTJ6IN88US=> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
SNU-423 NHPkN5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MY\JR|UxRTJwNEm3PFch|ryP M1LFW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
NCI-H2122 NWDIN|hQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDYV3FVUUN3ME2yMlI4Pjh2IN88US=> MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
RS4-11 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF;zfGpKSzVyPUGuPVU1OTVizszN NFXyeVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
HCC1395 NXXGUoxJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWT2UoFRUUN3ME2xMlc5Ojd|IN88US=> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
MIA-PaCa-2 NXfuXFBjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTFwN{W5O|ch|ryP M{XmRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
KYSE-180 NHfhbI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzRTWM2OD1zLkeyO|g5KM7:TR?= NUPVcIVMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
BCPAP MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnETWM2OD1zLk[2PFk5KM7:TR?= NHX6bmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
A498 NHfISIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVXxZ29xUUN3ME2xMlMxPDZ7IN88US=> M37mNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
TYK-nu NVvPUoFOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTFwMEe5NVIh|ryP MmS4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
D-247MG MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLRWppKSzVyPUCuPVQ5PTJizszN NUXld4FLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
S-117 NEj6eGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfJR|UxRTBwOUKxN|Eh|ryP NWP0c|N2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
D-336MG MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\UO2lEPTB;MD64OFYzKM7:TR?= NHOyU5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
LOXIMVI MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTBwNUi4N|Ih|ryP M2PSTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
769-P M{njWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfnTWxKSzVyPUCuOVg1QTlizszN NHG3NJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK|5USU6JRWK8M4E,
GCT M1X5bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{TybGlEPTB;MD6zNFY4OiEQvF2= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
SW982 M{OxcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkC1TWM2OD1yLkKxOFE3KM7:TR?= M1GwWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
ES-CCE M4\lfWZ2dmO2aX;uJGF{e2G7 NVHBdmw3PcLizszN MYOzJIQ> NWfJT5o1TE2VTx?= NYfrc|R[W3S{b37ncJkhcW6mdXPld{B1cGViYXP0bZZqfHlib3[gW451N2KndHGtZ4F1\W6rbjDwZZRpf2G7 MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd5OUO2OUc,OjR5N{mzOlU9N2F-
ES-D3 MYnGeY5kfGmxbjDBd5NigQ>? MXq1xsDPxE1? MVyzJIQ> NI\nPI1FVVOR M4TNcnN1em:wZ3z5JIlv\HWlZYOgeIhmKGGldHn2bZR6KG:oIGfueE9j\XSjLXPheIVvcW5icHH0bJdigQ>? NGDyNmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe3PVM3PSd-MkS3O|k{PjV:L3G+
ES-CCE NF;H[WVEgXSxdH;4bYMhSXO|YYm= M2DSNlExyqEQvF2= MVuzJIQ> NVjxOWRvTE2VTx?= M3HZVWlv\HWlZYOgcY9l\XKjdHWgZ5l1d3SxeHnjbZR6 MnfYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5N{mzOlUoRjJ2N{e5N|Y2RC:jPh?=
ES-D3 MWXDfZRwfG:6aXOgRZN{[Xl? MlzaNVDDqM7:TR?= NYXSSIZ{OyCm NEjsZZpFVVOR MYPJcoR2[2W|IH3v[IVz[XSnIHP5eI91d3irY3n0fUB4cXSqIFnDOVAhd2ZiND65JO69VQ>? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd5OUO2OUc,OjR5N{mzOlU9N2F-
Neural progenitor cells NHraeIFHfW6ldHnvckBCe3OjeR?= NYTIZmM5OyEQvF2= NGTDWXIyOCCm MlnvSG1UVw>? MnLFTY5lfWOnczDn[Y5memG2aX;uJI9nKG6ndYLhcEBxem:pZX7peI9zKGOnbHzz MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ|OECzOEc,OjR4M{iwN|Q9N2F-
A549 NGTLWGlCeG:ydH;zbZMhSXO|YYm= NH7KeZg2KML3TR?= NF73XpgzPCCq MlyxSG1UVw>? MXjJcoNz\WG|ZYOgZ4VtdCCjcH;weI9{cXN? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4N{[4PEc,OjR|Nke2PFg9N2F-
H157 MWfBdI9xfG:|aYOgRZN{[Xl? NUnJ[Xc{PSEEtV2= MYWyOEBp M17oemROW09? MoO4SI9meyCwb4Sgd4lodmmoaXPhcpRtgSCjZn\lZ5Qh[XCxcITvd4l{ NYnBcJhtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlc3QDhpPkK0N|Y4Pjh6PD;hQi=>
H460 MUDBdI9xfG:|aYOgRZN{[Xl? NGjiOWM2KML3TR?= MlvZNlQhcA>? NGXEcYhFVVOR M4HlemF1fGWwdXH0[ZMh[2WubDDhdI9xfG:|aYO= NGfpXGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2O|Y5QCd-MkSzOlc3QDh:L3G+
H157 NIjHSINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13VWVUhyrWP NH3WTYYzPCCq M{fJV2ROW09? NY\N[4t5WHKxbX;0[ZMh[2WubDDwdo9tcW[ncnH0bY9v MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4N{[4PEc,OjR|Nke2PFg9N2F-
H460 NUPGdlFpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX61JOK2VQ>? NEnpN3EzPCCq MUnEUXNQ NGjMcWNRem:vb4Tld{Bk\WyuIIDyc4xq\mW{YYTpc44> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4N{[4PEc,OjR|Nke2PFg9N2F-
embryonic stem cells (ESC) MX\GeY5kfGmxbjDBd5NigQ>? NGGxdGg{KML3TR?= M2mwd|EyKGR? M{faNmROW09? NHruVJVRem:vb4Tld{B{\WyoLYLlcoV4[Wxib3[gcoHEt3[nLYT5dIUhdW:3c3Wg[Y1jenmxbnnjJJN1\W1iY3XscJMhMEWVQzm= MorGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NEOzNVIoRjJ|OESzN|EzRC:jPh?=
DN3 MUTGeY5kfGmxbjDBd5NigQ>? MkCwNVAh|ryP NYjVe3BHQTZiaB?= NHTtZ3BFVVOR M4jSfWlv[3KnYYPld{Bk\WyuIHTleoVtd3CvZX70JIFv\CCycn;sbYZmemG2aX;u NWXoeZZIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NlY6QDlpPkKzOVI3QTh7PD;hQi=>
platelets Ml3SSpVv[3Srb36gRZN{[Xl? MlnJNVAh|ryP NUHWb5dqOTVibXnu MYDEUXNQ NXjpfnp1UW6lcnXhd4V{KHSqcn;tZolvNW2nZHnheIVlKHCuYYTlcIV1KGGpZ4Ll[4F1cW:wIHHu[EBCXFBic3XjdoV1cW:w NIK3bGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KzPVg4Pyd-MkOyN|k5Pzd:L3G+
hiPSC-NPs NWe4VWVHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnyfYpbO+LCid88US=> NUXuOHA2PzJiaB?= NXrBe5lMTE2VTx?= NX7GXmI2UW6lcnXhd4V{KGOnbHygdJJwdGmoZYLheIlwdsLi MnPOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEK2PFcoRjJ{NkSyOlg4RC:jPh?=
INS-1E NUTsbnZQTnWwY4Tpc44hSXO|YYm= M4HFS|IvPSEQvF2= M4LqVFI1KGh? MVPEUXNQ MULJcoR2[2W|IHGgdoVlfWO2aX;uJI9nKE6SMTDveoVz\XiycnXzd4lwdg>? M4rpNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK3O|A1Lz5{MkSyO|cxPDxxYU6=
PANC1 MnzoRZBweHSxc3nzJGF{e2G7 NGDn[3XDqDVizszN NHW4NZU4OiCq NF;FS3hFVVOR NGDKXllVemmpZ3Xyd{BidiCjcH;weI91cWNicnXzdI9ve2V? MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJyMUG4Okc,OjJ{MEGxPFY9N2F-
Endothelial cell(EC) M1S1OmZ2dmO2aX;uJGF{e2G7 NYnacGNGOyEQvF2= NUnFbYI2TE2VTx?= MofTVJJwdW:2ZYOg[Y5ld3SqZXzpZYwh[2WubDCoSWMqKGSrZn\ldoVvfGmjdHnvci=> M{\jelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEWyNFA3Lz5{MkC1NlAxPjxxYU6=
HMC1.2 NV;YWlQyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUezNEDPxE1? NEjLcGw4OiCq MmH0SG1UVw>? NGHueXNT\WS3Y3XzJINmdGxic4Xyeol3[Wx? MoK5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyM{mzNFEoRjJ{MEO5N|AyRC:jPh?=
Sca-1+ HPC MoTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojZNkDPxE1? NILK[Ig3KGR? NGH4fZZFVVOR NH[4UZRUgW6ncnfpd5Rq[2GubImgdJJwdW:2ZYOgV4NiNTFtwrDIVGMheHKxbHnm[ZJifGmxbjDhcoQh[2:ub375JIZwem2jdHnvckB1d2endHjldkB4cXSqIFXHSi=> M33nWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNkK0N|Q5Lz5{MU[yOFM1QDxxYU6=
Oct4/Sox2/Klf4-infected MEFs NYH2N2ZmTnWwY4Tpc44hSXO|YYm= NGribpMyOCEQvF2= MW[yPEBl NXHnbVNRTE2VTx?= MWnQdo9ud3SnczDNSWZ{KHKncILv[5JidW2rbnegWJJidnOmdXPl[EBjgSCRY4S0MEBUd3h{LDDhcoQhU2yoNB?= MknTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6M{mwOVUoRjF7OEO5NFU2RC:jPh?=
H4IIE MlXNSpVv[3Srb36gRZN{[Xl? NX3VcJRPOTBizszN NGL1WVMzPCCq MmTGSG1UVw>? MlXXVoVofWyjdHXzJO6zNWOjdHXubY4h[WO2aY\peJkh[W6mIGTJVmUh[2:wdHHpcolv\yCycn;tc5RmeiCjY4Tpeol1gQ>? NIXy[Fc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUO1NFE6PSd-MUWzOVAyQTV:L3G+
insulin receptor–expressing CHO-IR NUO3fYZiTnWwY4Tpc44hSXO|YYm= MV61NFAhdk1? Ml7zN|AhdWmw M3jlVGROW09? M3;VeGFkfGm4YYTld{BIdHmlb3flckBUgW62aHHz[UApT1Nr MoTBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ4ME[0PVcoRjF{NkC2OFk4RC:jPh?=
3T3-L1 NXz5RZhMTnWwY4Tpc44hSXO|YYm= NX2wNmd4OyEQvF2= MWi0JIg> MnHLSG1UVw>? MUTJcohq[mm2czDwdoVi\Gmyb3P5eIUh\GmoZnXy[Y51cWG2aX;u NUfEbI9[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKwOVUzODBpPkGyNFU2OjByPD;hQi=>
CHO-IR M4rkcWZ2dmO2aX;uJGF{e2G7 M2\LWFMxKG2rbh?= Ml3iSWM2OD1yLke3JO69VQ>? Mn3KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyMU[xNlEoRjJ7MEG2NVIyRC:jPh?=
HepG2 NHPMPYhHfW6ldHnvckBCe3OjeR?= NX\yS3FyOyCq MULFR|UxRTFwNTFOwG0> Mof6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{NkGwNlMoRjJ{Mk[xNFI{RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cleaved PARP / CD31 / VE-Cadherin / α-SMA / vimentin; 

PubMed: 30709379     


NCI-H460 cells were co-cultured with HUVECs in a 3D culture system, and then treated with 1 μM CHIR-99021, 10 μM or 20 μM Cisplatin, or a combination of both, for 24 h. Cells were harvested and immunoblotted with anti-cleaved PARP, anti-CD31, anti-VE-cadherin, anti-α-SMA, anti-vimentin, and anti-β-actin antibodies. 

CTNNB1; 

PubMed: 23993102     


Treatment with the GSK3β inhibitor CHIR-99021 led to increased levels of β-catenin in RKO and HT29 (non-mutant) cells.

β-catenin; 

PubMed: 24165128     


HeLa cells were treated with 5 μm CHIR-99021 for the indicated times. The samples were analyzed by Western blot.

30709379 23993102 24165128
Growth inhibition assay
Cell viability; 

PubMed: 22039301     


HMC-1.2 cells were cultured in the absence or presence of GSK3β inhibitor (CHIR 99021, 3–30 µM) and viability cell count was assessed by trypan blue dye exclusion after 24 h, 48 h or 72 h. All data are presented as the mean ± SEM of three independent experiments conducted in triplicate. p < 0.05*, p < 0.001** p < 0.0001*** vs vehicle treated control cells.

22039301
Immunofluorescence
SOX10 / EdU; 

PubMed: 28174705     


Incorporation of EdU (white arrowheads) into SOX10+ ENS (enteric nervous system) cells with and without CHIR-99021.

Oct3/4; 

PubMed: 24779365     


Fluorescence micrographs showing the protein expression of Brachyury/Oct3/4 after a three day differentiation of ES-D3 cells with BIO (0.5 μM), SB-216763 (5 μM), CHIR-99021 (5 μM), or CHIR-98014 (1 μM). Nuclei were counterstained with DAPI (blue). The merge is shown in yellow. Original magnification 400x. Scale bar = 50 μm.

28174705 24779365
In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. [1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. [4]

Protocol

Kinase Assay:

[1]

- Collapse

Cell-free kinase assays:

Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research:

[3]

- Collapse
  • Cell lines: INS-1E
  • Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM
  • Incubation Time: 1, or 4 days
  • Method:

    Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system.


    (Only for Reference)
Animal Research:

[1]

- Collapse
  • Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes
  • Dosages: ~48 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (185.33 mM)
Ethanol 2 mg/mL (3.98 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
24mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 501.8
Formula

C22H18Cl2N8.HCl

CAS No. 1797989-42-4
Storage powder
in solvent
Synonyms N/A
Smiles CC1=CN=C(N1)C2=CN=C(N=C2C3=C(C=C(C=C3)Cl)Cl)NCCNC4=NC=C(C=C4)C#N.Cl

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

  • Question 1:

    I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?

  • Answer:

    The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.

  • Question 2:

    How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?

  • Answer:

    Please change the culture medium each day to add the new comound in it if the duration is more than 72h.

  • Question 3:

    What is the difference between S2924 and S1263? Which one is better for differentiation?

  • Answer:

    S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID