CHIR-99021 (CT99021) HCl

For research use only.

Catalog No.S2924

207 publications

CHIR-99021 (CT99021) HCl Chemical Structure

CAS No. 1797989-42-4

CHIR-99021 (CT99021) HCl is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2. CHIR-99021 is a potent pharmacological activators of the Wnt/beta-catenin signaling pathway. CHIR-99021 significantly rescues light-induced autophagy and augments GR, RORα and autophagy-related proteins.

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Selleck's CHIR-99021 (CT99021) HCl has been cited by 207 publications

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description CHIR-99021 (CT99021) HCl is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2. CHIR-99021 is a potent pharmacological activators of the Wnt/beta-catenin signaling pathway. CHIR-99021 significantly rescues light-induced autophagy and augments GR, RORα and autophagy-related proteins.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. [1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). [2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
3T3-L1 NVXmU2JzTnWwY4Tpc44hSXO|YYm= NU\vVlFbOyEQvF2= NY\zZ41iPCCq MnvZSG1UVw>? MnjnTY5pcWKrdIOgdJJm[WSrcH;jfZRmKGSrZn\ldoVvfGmjdHnvci=> MUixNlA2PTJyMB?=
insulin receptor–expressing CHO-IR NVi1cmMyTnWwY4Tpc44hSXO|YYm= Mnn4OVAxKG6P MlHaN|AhdWmw NYjyN3lQTE2VTx?= MXfBZ5RqfmG2ZYOgS4x6[2:pZX6gV5lvfGijc3WgLGdUMQ>? Mn;nNVI3ODZ2OUe=
H4IIE M1LXWmZ2dmO2aX;uJGF{e2G7 MmLqNVAh|ryP MmHENlQhcA>? M4fWUGROW09? NF:ydFRT\We3bHH0[ZMh|rJvY3H0[Y5qdiCjY4Tpeol1gSCjbnSgWGlTTSClb370ZYlvcW6pIIDyc41wfGW{IHHjeIl3cXS7 MXOxOVM2ODF7NR?=
Oct4/Sox2/Klf4-infected MEFs NHP3b2NHfW6ldHnvckBCe3OjeR?= MoLXNVAh|ryP MX2yPEBl NHnFUlhFVVOR MYPQdo9ud3SnczDNSWZ{KHKncILv[5JidW2rbnegWJJidnOmdXPl[EBjgSCRY4S0MEBUd3h{LDDhcoQhU2yoNB?= NGjwS5IyQTh|OUC1OS=>
Sca-1+ HPC MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnTiNkDPxE1? NEfGeYo3KGR? NHXldWNFVVOR MkHCV5lv\XKpaYP0bYNidGy7IIDyc41wfGW|IGPjZU0yM8LiSGDDJJBzd2yrZnXyZZRqd25iYX7kJINwdG:weTDmc5Ju[XSrb36geI9o\XSqZYKge4l1cCCHR1[= M{\vR|IyPjJ2M{S4
HMC1.2 NXvXWJBLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXSzNEDPxE1? MnTMO|IhcA>? NIXVUVRFVVOR MomwVoVlfWOnczDj[YxtKHO3co\peoFt NF3pdHozOjB|OUOwNS=>
Endothelial cell(EC) NH\1OWxHfW6ldHnvckBCe3OjeR?= NIjK[3g{KM7:TR?= NIDGcXlFVVOR M3TEPXBzd22xdHXzJIVv\G:2aHXsbYFtKGOnbHygLGVEMSCmaX\m[ZJmdnSrYYTpc44> M{jQbVIzODV{MEC2
PANC1 NGn4PGdCeG:ydH;zbZMhSXO|YYm= M2fCeOKhPSEQvF2= NVr0UI9mPzJiaB?= MXXEUXNQ NHq4e3RVemmpZ3Xyd{BidiCjcH;weI91cWNicnXzdI9ve2V? MkW1NlIzODFzOE[=
INS-1E NHvZSZVHfW6ldHnvckBCe3OjeR?= NWnNWoNlOi53IN88US=> MoCyNlQhcA>? MYPEUXNQ MX\JcoR2[2W|IHGgdoVlfWO2aX;uJI9nKE6SMTDveoVz\XiycnXzd4lwdg>? MX6yNlQzPzdyNB?=
hiPSC-NPs NUj5WmViT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfFWFE{6oDLzszN NFvuZW44OiCq MXjEUXNQ M3fmd2lv[3KnYYPld{Bk\WyuIIDyc4xq\mW{YYTpc47DqA>? NE\CbYIzOjZ2Mk[4Oy=>
platelets NELzPWpHfW6ldHnvckBCe3OjeR?= MmLBNVAh|ryP MXOxOUBucW5? NFfn[WhFVVOR NXrsclhxUW6lcnXhd4V{KHSqcn;tZolvNW2nZHnheIVlKHCuYYTlcIV1KGGpZ4Ll[4F1cW:wIHHu[EBCXFBic3XjdoV1cW:w Mlr0NlMzOzl6N{e=
DN3 MW\GeY5kfGmxbjDBd5NigQ>? Mkn2NVAh|ryP M{jxRVk3KGh? MoKwSG1UVw>? MWfJcoNz\WG|ZYOgZ4VtdCCmZY\lcI9xdWWwdDDhcoQheHKxbHnm[ZJifGmxbh?= MXGyN|UzPjl6OR?=
embryonic stem cells (ESC) NVXSS4MzTnWwY4Tpc44hSXO|YYm= NVW2fpd5OyEEtV2= MYSxNUBl Mnq5SG1UVw>? M4CxbHBzd22xdHXzJJNmdGZvcnXu[ZdidCCxZjDuZeOwfmVvdInw[UBud3W|ZTDlcYJzgW:waXOgd5RmdSClZXzsd{ApTVOFKR?= NGG5dpAzOzh2M{OxNi=>
H460 NGrTb45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{HiWFUhyrWP M3jIOFI1KGh? M3P2RWROW09? NH3MSGxRem:vb4Tld{Bk\WyuIIDyc4xq\mW{YYTpc44> NU\G[ndwOjR|Nke2PFg>
H157 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYixTXJ6PSEEtV2= Ml\VNlQhcA>? MXXEUXNQ NXzWNI9DWHKxbX;0[ZMh[2WubDDwdo9tcW[ncnH0bY9v NWHaS2JiOjR|Nke2PFg>
H460 MkCwRZBweHSxc3nzJGF{e2G7 M{TmSlUhyrWP MXSyOEBp M1jCTmROW09? NVjWWmI1SXS2ZX71ZZRmeyClZXzsJIFxd3C2b4Ppdy=> NV3oc3Y{OjR|Nke2PFg>
H157 NGfkRndCeG:ydH;zbZMhSXO|YYm= NH:0e2I2KML3TR?= NF;yV3UzPCCq NYfiSGZ[TE2VTx?= NF\5VWxFd2W|IH7veEB{cWewaX\pZ4FvfGy7IHHm[oVkfCCjcH;weI9{cXN? NHvNNW4zPDN4N{[4PC=>
A549 NFvsR2hCeG:ydH;zbZMhSXO|YYm= NH\GTYQ2KML3TR?= NWLxVVZZOjRiaB?= NFu5dIpFVVOR MVvJcoNz\WG|ZYOgZ4VtdCCjcH;weI9{cXN? MlLZNlQ{Pjd4OEi=
Neural progenitor cells MYXGeY5kfGmxbjDBd5NigQ>? NIOxXnE{KM7:TR?= M1frRlExKGR? NULZeoI1TE2VTx?= M4rPTmlv\HWlZYOg[4Vv\XKjdHnvckBw\iCwZYXyZYwheHKxZ3XubZRweiClZXzsdy=> NH;VOJozPDZ|OECzOC=>
ES-D3 NHjzfHZEgXSxdH;4bYMhSXO|YYm= NIjuc3cyOMLizszN MknXN{Bl MorOSG1UVw>? NXXwcVljUW6mdXPld{Bud2SncnH0[UBkgXSxdH;4bYNqfHlid3n0bEBKSzVyIH;mJFQvQSEQvF2= M1nuVFI1Pzd7M{[1
ES-CCE M1XRcGN6fG:2b4jpZ{BCe3OjeR?= NYPwVJVEOTEEoN88US=> NGX5epc{KGR? MmLISG1UVw>? NWG1PZZ3UW6mdXPld{Bud2SncnH0[UBkgXSxdH;4bYNqfHl? NYPCZWwxOjR5N{mzOlU>
ES-D3 NXXae2hHTnWwY4Tpc44hSXO|YYm= Ml;nOeKh|ryP NUPNeHNHOyCm M3HmW2ROW09? NXPCe2dNW3S{b37ncJkhcW6mdXPld{B1cGViYXP0bZZqfHlib3[gW451N2KndHGtZ4F1\W6rbjDwZZRpf2G7 MYeyOFc4QTN4NR?=
ES-CCE NFztVm5HfW6ldHnvckBCe3OjeR?= NHn0Ulk2yqEQvF2= NULrc2FxOyCm MY\EUXNQ NGqzN4ZUfHKxbnfsfUBqdmS3Y3XzJJRp\SCjY4Tpeol1gSCxZjDXcpQw[mW2YT3jZZRmdmmwIIDheIh4[Xl? NVHET4tKOjR5N{mzOlU>
SW982 MmDhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkLsTWM2OD1yLkKxOFE3KM7:TR?= NV\3SG5IW0GQR1XS
GCT NVnPTYVET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVTSclQ2UUN3ME2wMlMxPjd{IN88US=> MnrjV2FPT0WU
769-P NHHjNmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlnyTWM2OD1yLkW4OFk6KM7:TR?= NEj3U|RUSU6JRWK=
LOXIMVI MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUiwPXVwUUN3ME2wMlU5QDN{IN88US=> Ml3EV2FPT0WU
D-336MG NUPsdmpIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVPJR|UxRTBwOES2NkDPxE1? NFfSeZFUSU6JRWK=
S-117 NGXz[5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\TTWM2OD1yLkmyNVMyKM7:TR?= NF\SdodUSU6JRWK=
D-247MG NIPUOVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzHTWM2OD1yLkm0PFUzKM7:TR?= MmDwV2FPT0WU
TYK-nu NX;s[Yp6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3fuRmlEPTB;MT6wO|kyOiEQvF2= MV3TRW5ITVJ?
A498 M{fJNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYfJR|UxRTFwM{C0Olkh|ryP MV7TRW5ITVJ?
BCPAP MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXLNN3pOUUN3ME2xMlY3QDl6IN88US=> M3Xob3NCVkeHUh?=
KYSE-180 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTFwN{K3PFgh|ryP M2nqVHNCVkeHUh?=
MIA-PaCa-2 NEXORZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXnmXIJpUUN3ME2xMlc2QTd5IN88US=> MlrXV2FPT0WU
HCC1395 NYLXepNkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTFwN{iyO|Mh|ryP NVLWVXdNW0GQR1XS
RS4-11 MnfuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYXKcoZnUUN3ME2xMlk2PDF3IN88US=> MV\TRW5ITVJ?
NCI-H2122 NH3kSI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3LkeGlEPTB;Mj6yO|Y5PCEQvF2= NEDpRVJUSU6JRWK=
SNU-423 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTJwNEm3PFch|ryP NFnVR2hUSU6JRWK=
SK-LMS-1 M4LsXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1HuSmlEPTB;Mj62OFEzQCEQvF2= MYXTRW5ITVJ?
RPMI-7951 NWL0b|VLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVnJR|UxRTJwN{CxPVMh|ryP MWjTRW5ITVJ?
COLO-829 M3znXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;xTWM2OD1{Lke2OVQh|ryP M4TJUHNCVkeHUh?=
SCC-9 M3jqUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWXJR|UxRTJwOUW0PFUh|ryP MWPTRW5ITVJ?
A704 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTNwMEixNkDPxE1? MWDTRW5ITVJ?
HOP-62 M{LKVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTNwM{[wOVQh|ryP NF7MfYlUSU6JRWK=
HCC1569 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkHNTWM2OD1|LkS4N|A2KM7:TR?= Ml:5V2FPT0WU
A172 NYjReGZXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MY\JR|UxRTNwN{W0PVgh|ryP M4j6bXNCVkeHUh?=
MOLT-16 M4S1[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\GTWM2OD1|Lki4OVU3KM7:TR?= MkjQV2FPT0WU
TE-15 NXm2PJJ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTNwOUWzOFMh|ryP NIK1V3lUSU6JRWK=
OE19 NXTwfG84T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXvBSWN[UUN3ME2zMlk3PDVzIN88US=> NH3YSI5USU6JRWK=
MHH-ES-1 NILaOGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRTRwMES1OFUh|ryP NEnMfY9USU6JRWK=
NKM-1 NGK2OJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLtNZU{UUN3ME20MlE{QTdzIN88US=> NFnqRXdUSU6JRWK=
RCC10RGB MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4CxNmlEPTB;ND6yPVQ4QSEQvF2= M1TsTnNCVkeHUh?=
BxPC-3 NUjR[VRsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPBTWM2OD12LkewN|kyKM7:TR?= NVTkVmE1W0GQR1XS
ALL-PO M3LQUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NW\jc3ZLUUN3ME20Mlg{QDV6IN88US=> M3vKWXNCVkeHUh?=
SK-OV-3 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTVTWM2OD12Lkm5N|Qh|ryP MWLTRW5ITVJ?
SW1710 NUnWcHlST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkXxTWM2OD13LkSyOFU3KM7:TR?= MYHTRW5ITVJ?
NCI-H2452 NHfqVlhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGHrO2lKSzVyPUWuOFU3OjhizszN NEi2VoJUSU6JRWK=
D-392MG NULwTZN{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLEW4tKSzVyPUWuOFkyPDVizszN MYHTRW5ITVJ?
LB2518-MEL NXL0O2liT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Moq0TWM2OD13LkeyOlI4KM7:TR?= MWnTRW5ITVJ?
BL-41 NWPTNGcyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlrmTWM2OD13LkixNlY3KM7:TR?= MVfTRW5ITVJ?
WM-115 NF;1OnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEHJdoxKSzVyPUWuPFE2ODlizszN NX24eYpyW0GQR1XS
SK-MEL-2 NV7kbVY3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkWxTWM2OD14LkO4N|Q1KM7:TR?= MoLiV2FPT0WU
CGTH-W-1 M1HtN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTZwNEG3JO69VQ>? MXnTRW5ITVJ?
BB65-RCC NH3zeXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG\6XYZKSzVyPU[uOVMyQTVizszN M2XLWnNCVkeHUh?=
CAL-85-1 Ml\lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HJNmlEPTB;Nj62NFk{PiEQvF2= NVLoSJVDW0GQR1XS
RT-112 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjyTWM2OD15LkG2OFU3KM7:TR?= NELGeGhUSU6JRWK=
MC116 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH\QOWVKSzVyPUeuNlg3QTFizszN NYrtdXFQW0GQR1XS
G-361 NG\P[4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGHqbXdKSzVyPUeuOFY2PjFizszN NFrRNJVUSU6JRWK=
SW1417 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfJTGFxUUN3ME23MlY1PTh2IN88US=> M{D3[HNCVkeHUh?=
MV-4-11 NIDE[lhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1jpNGlEPTB;OD61OFMxPCEQvF2= M1v0UXNCVkeHUh?=
CAL-72 M4XXXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUTKVW5QUUN3ME24Mlc3OTF|IN88US=> MWrTRW5ITVJ?
MKN28 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIDORVBKSzVyPUiuPFg2OTZizszN NVPrfXFvW0GQR1XS
MEG-01 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF6wcWhKSzVyPUiuPVkxPDNizszN MVfTRW5ITVJ?
ACHN MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2LIOWlEPTB;OT6zNFU1PiEQvF2= M3Gwc3NCVkeHUh?=
NCI-H1755 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvtTWM2OD17LkO5PVg3KM7:TR?= NVzJeWU4W0GQR1XS
NCI-H64 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4L3RWlEPTB;OT60NlI2PSEQvF2= NVLXZ3Y{W0GQR1XS
COLO-205 NU\nOZpIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmWyTWM2OD17LkSzPFIyKM7:TR?= NHrIb3RUSU6JRWK=
CTV-1 MonJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFmxcFJKSzVyPUmuOFM5QTRizszN NX\sW3l4W0GQR1XS
U-2-OS NWjGfZBWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUXJR|UxRTlwNEWwNFYh|ryP MV;TRW5ITVJ?
LB996-RCC MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrlTWM2OD17LkWyN|k4KM7:TR?= NYq0[|JvW0GQR1XS
SK-N-DZ M2K4Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{nhU2lEPTB;OT62OVc1OiEQvF2= MoLFV2FPT0WU
EFM-19 NXrPOmsyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWLJR|UxRTlwN{W1OlYh|ryP MkDJV2FPT0WU
DBTRG-05MG NH\xc41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTlwN{e3O{DPxE1? NEDJOGFUSU6JRWK=
U-118-MG NVvjU2RtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX\JR|UxRTlwOUS0OFQh|ryP MUPTRW5ITVJ?
LB1047-RCC NILmNY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjIR2VKSzVyPUmuPVk4QThizszN M1fDV3NCVkeHUh?=
NCI-H28 M1LTS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;GPGlEPTB;MUCuOFQyOyEQvF2= NIHKXmFUSU6JRWK=
MZ2-MEL MnrYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTFyLkS2O|ch|ryP M2qwcHNCVkeHUh?=
M059J NVSwSnRST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTFyLk[xNFIh|ryP Mmi4V2FPT0WU
HT-3 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4nnZWlEPTB;MUCuOlE4QSEQvF2= NIf2NHpUSU6JRWK=
SNU-387 M2PpcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTFyLkeyN|Uh|ryP M2L2RXNCVkeHUh?=
NCI-H526 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYP0O3V3UUN3ME2xNU4yOzZ3IN88US=> NF3CPIhUSU6JRWK=
HEL NUW5SIVST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1PJ[2lEPTB;MUGuOlU4QSEQvF2= MULTRW5ITVJ?
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BHT-101 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEXRbm5KSzVyPUG0NE4zODhizszN NWDRPYM1W0GQR1XS
OVCAR-4 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoPFTWM2OD1zNECuO|Q6KM7:TR?= MnnIV2FPT0WU
Detroit562 Mk\uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;kRoFKSzVyPUG0NU41PjZizszN NGT3ZXVUSU6JRWK=
OCI-AML2 NFL0UlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFHrWWVKSzVyPUG0NU42ODdizszN NVLqe|FjW0GQR1XS
OE33 NEDCWZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2W3emlEPTB;MUSxMlc6QCEQvF2= MkniV2FPT0WU
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NCI-H810 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3q0UWlEPTB;MUSzMlY5PyEQvF2= NULKSmNyW0GQR1XS
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HTC-C3 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRTF2NT6wO|Uh|ryP MnPQV2FPT0WU
G-401 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTF2NT64N|Uh|ryP MnnFV2FPT0WU
OMC-1 NIXvcZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTF2NT65Olch|ryP M3Tad3NCVkeHUh?=
LK-2 NFHsU2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTF2Nz6xOFMh|ryP M{fDTnNCVkeHUh?=
SW13 M33RXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4T4bmlEPTB;MUS3MlI2OyEQvF2= NH7NOVNUSU6JRWK=
BB49-HNC MkjiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4mzbWlEPTB;MUS3MlQxPCEQvF2= NGPHfnVUSU6JRWK=
DMS-114 NGqze3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jIZWlEPTB;MUS3MlQ1OSEQvF2= NVvlUJNrW0GQR1XS
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GAMG MlP5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTF2OD6yOFch|ryP M4PtZnNCVkeHUh?=
KYSE-520 NETm[4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXIO49KSzVyPUG0PE42PCEQvF2= NVvMRVlDW0GQR1XS
LB647-SCLC NIfo[lVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjBTWM2OD1zNEiuOVYzKM7:TR?= MnOyV2FPT0WU
639-V NXzKbYR7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmnkTWM2OD1zNEiuO|A2KM7:TR?= MlfVV2FPT0WU
TE-9 MlL1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2f0fmlEPTB;MUS5MlQ1OyEQvF2= NWO5UXFrW0GQR1XS
Caov-3 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTF2OT63NVQh|ryP MnzJV2FPT0WU
L-540 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\VXIozUUN3ME2xOFkvPzVizszN MYXTRW5ITVJ?
NCI-H748 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTF3MD6yNFkh|ryP MVrTRW5ITVJ?
GDM-1 MlTDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFyzNppKSzVyPUG1NU4yOzVizszN MVTTRW5ITVJ?
GAK M{KwbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;kTWM2OD1zNUGuN|c1KM7:TR?= NYDrcGg2W0GQR1XS
AsPC-1 NH3xUXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWjJR|UxRTF3Mj63OlQh|ryP NXLiS41{W0GQR1XS
MDA-MB-361 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVuySoZmUUN3ME2xOVMvOTZ2IN88US=> NH20dXZUSU6JRWK=
TT MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUXYTWRrUUN3ME2xOVMvPDlizszN NHXlVXNUSU6JRWK=
Calu-1 NG\RcVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTF3ND6wNFMh|ryP NIXKXYdUSU6JRWK=
RERF-LC-MS M2DrbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVjJR|UxRTF3ND63O|Ih|ryP NETueJdUSU6JRWK=
DK-MG NG\3coJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX7JR|UxRTF3NT6xPFMh|ryP M2LsTnNCVkeHUh?=
CAL-120 NUXMd|QzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4fQNmlEPTB;MUW1MlgzOSEQvF2= MUnTRW5ITVJ?
HCC1419 NVHvRnp2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlzPTWM2OD1zNU[uNFc4KM7:TR?= MlvpV2FPT0WU
DG-75 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX\JR|UxRTF3Nj62PVQh|ryP NYjJ[mV1W0GQR1XS
HCC38 MlfQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVvH[mFVUUN3ME2xOVcvOzZ7IN88US=> Ml;MV2FPT0WU
HCC70 NETscW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUns[VBoUUN3ME2xOVkvOjR{IN88US=> M3jSVXNCVkeHUh?=
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NCI-H2052 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXXfoZQUUN3ME2xOVkvPTh|IN88US=> MXjTRW5ITVJ?
EFO-27 MlzSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUe1VZNKUUN3ME2xOlAvPTh2IN88US=> NYLuemc3W0GQR1XS
CAL-62 NUTlcXFqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWfZR2VGUUN3ME2xOlIvPjF|IN88US=> MUXTRW5ITVJ?
CHP-212 NU\E[YJuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlHITWM2OD1zNkOuOlI3KM7:TR?= NH3BXItUSU6JRWK=
AU565 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{XFZ2lEPTB;MU[zMlY1KM7:TR?= MXXTRW5ITVJ?
Ca9-22 Mn\MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTF4ND6zNlEh|ryP MVTTRW5ITVJ?
EFO-21 NU\GN3NbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWTCfHVjUUN3ME2xOlUvPDVizszN MYHTRW5ITVJ?
KINGS-1 M3jhWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NELFb2RKSzVyPUG2O{4{PTRizszN M{\KTHNCVkeHUh?=
JVM-2 NHjJPWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmLETWM2OD1zNkeuOFUyKM7:TR?= MnfCV2FPT0WU
OVCAR-5 NIe2XIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTF4OD6zNFkh|ryP MX7TRW5ITVJ?
Calu-3 NYXyOFQ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWrQemZCUUN3ME2xOlgvOzFzIN88US=> M1S1XHNCVkeHUh?=
CP66-MEL NHH6WFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\PfGlEPTB;MU[4MlQyPSEQvF2= M4nTfnNCVkeHUh?=
KYSE-150 NHL4TFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTF4OT62N|Yh|ryP NUHSeZZkW0GQR1XS
Daoy Mn72S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1KxV2lEPTB;MU[5Mlg6OSEQvF2= NFW4SIlUSU6JRWK=
HUTU-80 NXrxSmY4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml7nTWM2OD1zN{GuNFMzKM7:TR?= MXTTRW5ITVJ?
SW620 MnL6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHzkZ3JKSzVyPUG3NU42OzVizszN MXfTRW5ITVJ?
HuO-3N1 NIm5SJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTF5Mj6yO|Mh|ryP NEW2NnhUSU6JRWK=
HH NX7lR5JjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGOwN4FKSzVyPUG3N{45PDhizszN MnPJV2FPT0WU
ME-180 NGW1OFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXrXVlRSUUN3ME2xO|QvODF4IN88US=> NVTjfmJlW0GQR1XS
SW756 NU\heotNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXzJR|UxRTF5ND64OlMh|ryP M1HqSnNCVkeHUh?=
LNCaP-Clone-FGC M3OzNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXvJR|UxRTF5NT64OlEh|ryP MYfTRW5ITVJ?
YT M1PTeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1rUR2lEPTB;MUe2MlM6QSEQvF2= M2PmcnNCVkeHUh?=
EW-3 Ml7KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkD0TWM2OD1zN{[uOVU2KM7:TR?= MYnTRW5ITVJ?
COLO-741 M4\jOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkLkTWM2OD1zN{euOVYyKM7:TR?= NYfwfXBIW0GQR1XS
SK-NEP-1 NIf3XGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\D[JhUUUN3ME2xO|cvQDB4IN88US=> M2W2c3NCVkeHUh?=
NB10 NF;JSYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7NbIFvUUN3ME2xO|cvQDR4IN88US=> M1;UWXNCVkeHUh?=
SF126 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jxS2lEPTB;MUe5MlQ{OiEQvF2= NYnQTWt1W0GQR1XS
NCI-H522 MoHJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rsdGlEPTB;MUixMlA2QSEQvF2= MnLXV2FPT0WU
PSN1 NIqxXYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2D2d2lEPTB;MUixMlY3KM7:TR?= NWizZ25vW0GQR1XS
GI-ME-N MmrUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHv3SXlKSzVyPUG4NU43PzJizszN NWHyOJBFW0GQR1XS
SBC-5 M3jOVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4PCTGlEPTB;MUixMlk{PCEQvF2= NFPzc3lUSU6JRWK=
ONS-76 NGj0WG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfjTWM2OD1zOEKuN|g4KM7:TR?= M3nDenNCVkeHUh?=
LOUCY NGW1S|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLCTIc5UUN3ME2xPFIvPjh6IN88US=> MVPTRW5ITVJ?
SN12C M4DhPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;xTWM2OD1zOEKuPFI6KM7:TR?= MXzTRW5ITVJ?
A427 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYLJR|UxRTF6ND62N|Eh|ryP NYL4cot{W0GQR1XS
DOK NFjsZo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnnHTWM2OD1zOESuOlQ{KM7:TR?= MknmV2FPT0WU
MDA-MB-415 NFnBPFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoHmTWM2OD1zOESuOlUyKM7:TR?= Ml2wV2FPT0WU
MRK-nu-1 NUTzUpFMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTF6ND65O|Ih|ryP NX;RU217W0GQR1XS
LB2241-RCC NGfTToNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1XZTGlEPTB;MUi1MlE1PSEQvF2= Mn\ZV2FPT0WU
NCI-H1563 NHP2UmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvuWmVKSzVyPUG4OU4zODlizszN NHzENnZUSU6JRWK=
MDA-MB-453 NFfCN3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTJSJB3UUN3ME2xPFYvQTRzIN88US=> MYfTRW5ITVJ?
MONO-MAC-6 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHFbGtKSzVyPUG4O{4{QTJizszN NHnxb3dUSU6JRWK=
CAPAN-1 NFrHb3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;NN2lEPTB;MUi3MlQ2OyEQvF2= NXmwToJEW0GQR1XS
SK-LU-1 NYnyXVVET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmPUTWM2OD1zOEiuOFI5KM7:TR?= NX3MeXdsW0GQR1XS
NCI-H1299 MmnVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mny0TWM2OD1zOEiuO|E6KM7:TR?= MVvTRW5ITVJ?
KG-1 NVfMT3ZUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXO0S|Z[UUN3ME2xPFkvPzl6IN88US=> MkXWV2FPT0WU
ETK-1 M3vhWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4P4UWlEPTB;MUmwMlE5OSEQvF2= M3n2WnNCVkeHUh?=
NCI-H838 Mm\VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HucGlEPTB;MUmwMlM1KM7:TR?= MlrJV2FPT0WU
HCE-4 NF35U2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjlR4J4UUN3ME2xPVAvQTl{IN88US=> NHf6T2NUSU6JRWK=
A2780 NXfMTZJbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTF7Mj64PVEh|ryP NFizb5VUSU6JRWK=
COLO-668 Mlq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkD3TWM2OD1zOUOuNVU4KM7:TR?= MkHJV2FPT0WU
HGC-27 MoPpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjoTWM2OD1zOUWuOVM5KM7:TR?= Mlv6V2FPT0WU
SW1088 NH\5WoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTF7NT62O|ch|ryP MkDtV2FPT0WU
KOSC-2 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV3JR|UxRTF7NT64NVgh|ryP MWnTRW5ITVJ?
TE-1 NUPxN2tNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnrwTWM2OD1zOU[uNlYh|ryP MYPTRW5ITVJ?
SW780 MlLlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\DTWM2OD1zOU[uO|M1KM7:TR?= MWfTRW5ITVJ?
22RV1 Mn\WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvuT|JKSzVyPUG5O{41OjRizszN MWXTRW5ITVJ?
IA-LM MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPzTWM2OD1zOUeuPFIzKM7:TR?= NITIOI1USU6JRWK=
TE-5 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlv6TWM2OD1zOUiuNFM{KM7:TR?= MnXqV2FPT0WU
JVM-3 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fWbGlEPTB;MUm4MlQyQSEQvF2= NXrQW3Y4W0GQR1XS
HT55 NIrq[WtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXK1[ZpLUUN3ME2xPVkvOTl{IN88US=> MY\TRW5ITVJ?
QIMR-WIL NEDDcGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlP6TWM2OD1{MECuNVE3KM7:TR?= M2nFdnNCVkeHUh?=
SJRH30 M37C[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTJyMD61NVUh|ryP NV;EcI04W0GQR1XS
COLO-679 NHXGR4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3\ndWlEPTB;MkCwMlg2QSEQvF2= MoDYV2FPT0WU
MDA-MB-231 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfDe3p4UUN3ME2yNFAvQTB{IN88US=> M3zQOXNCVkeHUh?=
MFM-223 NVrFPHhiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUXa[nVxUUN3ME2yNFEvPTh5IN88US=> NEXRTYxUSU6JRWK=
MFE-280 M3LvVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVz1OlVlUUN3ME2yNFEvQTZzIN88US=> MnrsV2FPT0WU
LAMA-84 M37DNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzpN2xKSzVyPUKwNk4xPzNizszN Mlm1V2FPT0WU
LS-1034 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTJyMj61OVMh|ryP Ml7DV2FPT0WU
NCI-H345 M2TKNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFHOWWpKSzVyPUKwN{4xOjdizszN NW\EZW5JW0GQR1XS
KYSE-70 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3ezRWlEPTB;MkCzMlg4QSEQvF2= MmTIV2FPT0WU
EGI-1 NGSxToRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmHnTWM2OD1{MESuPFM{KM7:TR?= NIn4NYZUSU6JRWK=
RPMI-2650 NWi3b|hCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3UT5EzUUN3ME2yNFUvPDZ5IN88US=> M3rzOHNCVkeHUh?=
NCI-H1651 MnntS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTJyNj63N{DPxE1? NVPVV4x5W0GQR1XS
HPAF-II NHuyfIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M36wT2lEPTB;MkC5MlY4OSEQvF2= NWXYXIRxW0GQR1XS
NUGC-3 NIDI[oZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2O5U2lEPTB;MkC5MlY4QCEQvF2= MmXyV2FPT0WU
DJM-1 M37hdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYDZbndsUUN3ME2yNFkvQDl5IN88US=> MV3TRW5ITVJ?
MHH-PREB-1 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rFXmlEPTB;MkGwMlM5OSEQvF2= MofBV2FPT0WU
EW-18 NHHIT|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHiTWM2OD1{MUCuO|Y{KM7:TR?= MUfTRW5ITVJ?
BT-20 M4LPVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFn2PGNKSzVyPUKxNE46QDZizszN M2jXV3NCVkeHUh?=
A431 M{XET2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLzWIlyUUN3ME2yNVEvOjl7IN88US=> Ml;0V2FPT0WU
TCCSUP NVT5S2lyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYj6bYpHUUN3ME2yNVEvPTN|IN88US=> NFyxUFRUSU6JRWK=
HO-1-N-1 NYDxcXRLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPpTWM2OD1{MUKuPFk1KM7:TR?= NGToSm5USU6JRWK=
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NCI-H2030 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;LdFBxUUN3ME2zOFEvPjB7IN88US=> Ml;rV2FPT0WU
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KP-4 NHnoeIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTN2Mz63N|Mh|ryP MUjTRW5ITVJ?
PF-382 M1jtXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jSS2lEPTB;M{S0MlQzOiEQvF2= M2XXT3NCVkeHUh?=
NB13 NGfnSZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV7JR|UxRTN2NT61PEDPxE1? NUfQdYc2W0GQR1XS
D-502MG NVKzRpBrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIq2SoZKSzVyPUO0OU45OTJizszN NUnQRmd3W0GQR1XS
CAL-54 NW\DdXZPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTN2NT64O|kh|ryP MX\TRW5ITVJ?
DOHH-2 M{PoWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUjiNIJPUUN3ME2zOFYvPjVizszN NUL5WWNIW0GQR1XS
SK-PN-DW Ml64S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NILNUmFKSzVyPUO0O{41OjZizszN NIfSN2pUSU6JRWK=
NCI-H720 M3;odmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfL[Zk5UUN3ME2zOFkvQDR4IN88US=> NV\DfIR1W0GQR1XS
SW1990 NX\4PIN2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIrzRpNKSzVyPUO1NU4zQCEQvF2= MYnTRW5ITVJ?
NCI-H2196 NF3k[WhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVrJR|UxRTN3Mj62NlQh|ryP NVL3e|Y6W0GQR1XS
P30-OHK NHTLOmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGewVGpKSzVyPUO1N{4xPjNizszN NX\idm1qW0GQR1XS
NCI-H358 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MniwTWM2OD1|NUWuN|U3KM7:TR?= MUTTRW5ITVJ?
CW-2 MnPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF76fIRKSzVyPUO1Ok42OjlizszN MYrTRW5ITVJ?
COLO-678 M1LZemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVvJR|UxRTN3Nz64OlMh|ryP MlvoV2FPT0WU
NCI-H2126 NF;OVJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjEeodKSzVyPUO1PE42OjFizszN MUTTRW5ITVJ?
DMS-53 NX7t[XBlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zKV2lEPTB;M{W4MlcyOSEQvF2= NWThOGhQW0GQR1XS
NCI-H1838 M4fMN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2HrNmlEPTB;M{W5Mlk5PyEQvF2= M2TrcnNCVkeHUh?=
SJSA-1 NUfYTGhTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTN4MD65N|kh|ryP NUPWepE1W0GQR1XS
ChaGo-K-1 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3T0OWlEPTB;M{[zMlkzPCEQvF2= NVi0UHJCW0GQR1XS
IST-SL2 NXzHUGZGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWPJR|UxRTN5Mj65NlYh|ryP MkPpV2FPT0WU
U-266 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEmzcXJKSzVyPUO3N{4yOzJizszN M3XWOXNCVkeHUh?=
P31-FUJ MmLpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTN5Nj65OlQh|ryP NXzOfINEW0GQR1XS
HCC2157 M1H6dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEP1bZVKSzVyPUO3PE4yPjFizszN MlLrV2FPT0WU
COR-L88 MnTyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEG5XG1KSzVyPUO4N{43PzdizszN NUXhOXVbW0GQR1XS
NCI-H596 M1;EVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zJT2lEPTB;M{i1MlM1KM7:TR?= NFr6R5BUSU6JRWK=
GOTO MnXBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF3TNpRKSzVyPUO5NE43PDFizszN M{PSTnNCVkeHUh?=
NCI-H719 Mn\4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTN7MT64PVMh|ryP NUfDeYxDW0GQR1XS
MN-60 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEfpdoxKSzVyPUO5N{46PDhizszN MknqV2FPT0WU
IST-SL1 M4mzbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHTxUm9KSzVyPUO5OE41ODZizszN NF3pbHRUSU6JRWK=
TE-6 Mn:3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHZTWM2OD1|OUeuNlU1KM7:TR?= MorBV2FPT0WU
SW962 M3vnPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmW0TWM2OD1|OUiuOlkh|ryP M4fUTXNCVkeHUh?=
FADU M2XobWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGnpbHlKSzVyPUSwN{4xQDNizszN NVT2[JFFW0GQR1XS
ECC4 NYjhdmU3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHfoSpdKSzVyPUSwPE41PThizszN MWXTRW5ITVJ?
MES-SA NG\STFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPpPWVKSzVyPUSwPU46QCEQvF2= M4DFW3NCVkeHUh?=
NCI-H716 NFjISm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUDneWxsUUN3ME20NVAvODd3IN88US=> M3\jW3NCVkeHUh?=
GR-ST NGXOWZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHVTWM2OD12MUCuOFk4KM7:TR?= MV7TRW5ITVJ?
KY821 M3XkW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvuTppKSzVyPUSxOE4xQDZizszN MV\TRW5ITVJ?
NCI-H2141 MnHrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFnnN29KSzVyPUSyOE4{QDhizszN NXGzN4dGW0GQR1XS
MDA-MB-134-VI NWfSW2dET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\ROHVyUUN3ME20N|EvODN|IN88US=> MnixV2FPT0WU
RH-1 NInUUldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXn[ZRKSzVyPUSzOU4xQSEQvF2= M1vPZ3NCVkeHUh?=
AM-38 MlP2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vSPGlEPTB;NEO4MlU1KM7:TR?= M4\JO3NCVkeHUh?=
BL-70 M4Xib2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvqbGFUUUN3ME20N|kvPDJ7IN88US=> NWr6RmNSW0GQR1XS
COLO-684 Mor3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFPiZYVKSzVyPUS0PE45QDhizszN NVi3OXdQW0GQR1XS
DMS-79 MljVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUnJR|UxRTR2OT62PEDPxE1? MlPTV2FPT0WU
EW-1 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojUTWM2OD12NUKuPFg5KM7:TR?= NU\sV5BLW0GQR1XS
MS-1 MlvvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3OZmRKSzVyPUS1OE4{QDVizszN M3rIOHNCVkeHUh?=
A4-Fuk MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTR3ND61NFch|ryP NIXCXZhUSU6JRWK=
TALL-1 MlznS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlfLTWM2OD12NUWuNlk4KM7:TR?= M{\EbnNCVkeHUh?=
SCLC-21H M1TuS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHXQN5RKSzVyPUS1OU43QTlizszN MWfTRW5ITVJ?
KMS-12-PE MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\2dnFrUUN3ME20OVYvQDJ{IN88US=> NWPue|l5W0GQR1XS
TE-441-T MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxRTR3Nz6xPFYh|ryP MoTZV2FPT0WU
A3-KAW MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXJemZVUUN3ME20OlAvQDRzIN88US=> NGrr[Y1USU6JRWK=
NCI-H1092 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoK3TWM2OD12NkOuPVQ{KM7:TR?= NFjh[WpUSU6JRWK=
COR-L279 M4fpSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrveopYUUN3ME20OlQvQDR5IN88US=> NV;IcZFxW0GQR1XS
NCI-SNU-5 NHfSO29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml3sTWM2OD12N{KuNlgh|ryP MVjTRW5ITVJ?
JAR MmnRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWW0[HVEUUN3ME20O|QvPzh6IN88US=> NHvS[G9USU6JRWK=
NCI-H446 MoK0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEH4U49KSzVyPUS3O{4xQCEQvF2= NXXk[WFmW0GQR1XS
HDLM-2 NELmUpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXrcYFGUUN3ME20PFAvQDZ7IN88US=> MorJV2FPT0WU
D-283MED NFHIXIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDsTWM2OD12OEiuPVI1KM7:TR?= NX;Gb4lrW0GQR1XS
NCI-H1395 M3XMW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo[wTWM2OD12OEmuNFA1KM7:TR?= MXfTRW5ITVJ?
RPMI-6666 M3HqdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmCwTWM2OD12OUGuN|k5KM7:TR?= M3nLeXNCVkeHUh?=
CPC-N MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETsPWJKSzVyPUWwNk4xPjNizszN MVvTRW5ITVJ?
EC-GI-10 M4X1ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1i3bGlEPTB;NUCyMlE3QSEQvF2= MV7TRW5ITVJ?
CA46 M{fNNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV2wVINNUUN3ME21NFQvPDB5IN88US=> Mn\kV2FPT0WU
EHEB MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;UTWM2OD13MEeuNlA5KM7:TR?= NWTVcZJiW0GQR1XS
RPMI-8402 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1XVZ2lEPTB;NUGwMlAzOSEQvF2= NGrmXFJUSU6JRWK=
DU-4475 NVjCfGZTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2nENGlEPTB;NUGxMlExOSEQvF2= NYflOlkyW0GQR1XS
P12-ICHIKAWA MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUXlfYFPUUN3ME21NVEvPDF4IN88US=> NWrsb5Y3W0GQR1XS
NCI-H1694 NV3Fd21wT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;ITHBxUUN3ME21NVIvPDN7IN88US=> NXPKRYtlW0GQR1XS
NCI-H2171 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4O1Z2lEPTB;NUGyMlcxPSEQvF2= MVPTRW5ITVJ?
Mo-T M13jRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEnNeJZKSzVyPUWxOU4yOTZizszN NHPLN5NUSU6JRWK=
HL-60 M2L0R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjLTWM2OD13MkKuPVI1KM7:TR?= NY\EcI53W0GQR1XS
NCI-H1436 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M172VWlEPTB;NUKzMlA3PCEQvF2= MXHTRW5ITVJ?
NCI-H510A MnTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPy[m1KSzVyPUWyOE43QTZizszN NV7PSXJIW0GQR1XS
NCI-H2081 M1fMWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\rSWlEPTB;NUK1MlUyOyEQvF2= NEPER3ZUSU6JRWK=
NB5 Mn7GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\zNIE5UUN3ME21NlYvPDNizszN MYjTRW5ITVJ?
NCI-H2227 NWrqXng6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzoOI9KSzVyPUWzNE4zPTRizszN MnvqV2FPT0WU
HSC-4 M2[4b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTV|OT6yPVMh|ryP NIPBdldUSU6JRWK=
RL NFziZ|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTV2Mj60OFIh|ryP M{[yfnNCVkeHUh?=
EVSA-T M2P5WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\0TWM2OD13NEOuOlg1KM7:TR?= NGD3NopUSU6JRWK=
NCI-H889 NHPOdY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILhdZFKSzVyPUW0O{4zOyEQvF2= M{PXNHNCVkeHUh?=
COLO-320-HSR M1LD[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvEfJdmUUN3ME21OVEvOzVizszN Ml;BV2FPT0WU
EW-11 MlnUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTV3Mj6zPVkh|ryP M{nwR3NCVkeHUh?=
UACC-812 NH\Jd2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jwTGlEPTB;NUW5MlM6OSEQvF2= NF3nVoRUSU6JRWK=
CAL-148 NVfu[nl{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2jKVGlEPTB;NU[1MlA5QSEQvF2= NHf6XZdUSU6JRWK=
K052 NGm2NJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWX3dHByUUN3ME21O|kvOjF|IN88US=> MYLTRW5ITVJ?
L-428 NIrKW2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTZyMD62O|Eh|ryP MVnTRW5ITVJ?
LP-1 Mln0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;aU4hxUUN3ME22NFMvPTh5IN88US=> NV3DVYp2W0GQR1XS
ML-2 NF[zb4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH30cHpKSzVyPU[wOk42PzlizszN MYXTRW5ITVJ?
KARPAS-45 M4fKS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTRTHJuUUN3ME22NVgvOjJzIN88US=> NIrM[pRUSU6JRWK=
HT NYX4XXczT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LP[2lEPTB;NkKyMlc5PSEQvF2= M4fnWnNCVkeHUh?=
NCI-H226 MkjtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTZ{OD6zOVMh|ryP M3jr[nNCVkeHUh?=
L-363 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlP1TWM2OD14NEiuNFY1KM7:TR?= M2PBXXNCVkeHUh?=
IM-9 MlfzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFzwV|dKSzVyPU[1NU42PDFizszN MmXuV2FPT0WU
TUR MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PkNWlEPTB;NkexMlMzOiEQvF2= MoXZV2FPT0WU
NCI-SNU-16 NV;nRpo6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTZ6MD60OlIh|ryP NX;OTZBMW0GQR1XS
NCI-SNU-1 Mn3iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTZ7MD6xO|Ih|ryP MnLEV2FPT0WU
U-87-MG MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{ftO2lEPTB;Nkm0MlU5PSEQvF2= M4nWeHNCVkeHUh?=
KM-H2 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17Kc2lEPTB;Nkm5MlA2QSEQvF2= NUXjNlBTW0GQR1XS
DEL MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIm4ZW1KSzVyPUewNE45OzhizszN MWPTRW5ITVJ?
KARPAS-422 NIHsN4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;oTWM2OD15OEmuN|E2KM7:TR?= MkK0V2FPT0WU
BC-3 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2j1dWlEPTB;OEC1MlIxOyEQvF2= MXHTRW5ITVJ?
NCI-H1155 NIe1UGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk[0TWM2OD16MUiuNFU6KM7:TR?= NUPX[29oW0GQR1XS
NCI-H1581 NVrFW3psT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIP5U4ZKSzVyPUmwNE4zOzFizszN NIfhcGVUSU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cleaved PARP / CD31 / VE-Cadherin / α-SMA / vimentin; 

PubMed: 30709379     


NCI-H460 cells were co-cultured with HUVECs in a 3D culture system, and then treated with 1 μM CHIR-99021, 10 μM or 20 μM Cisplatin, or a combination of both, for 24 h. Cells were harvested and immunoblotted with anti-cleaved PARP, anti-CD31, anti-VE-cadherin, anti-α-SMA, anti-vimentin, and anti-β-actin antibodies. 

CTNNB1; 

PubMed: 23993102     


Treatment with the GSK3β inhibitor CHIR-99021 led to increased levels of β-catenin in RKO and HT29 (non-mutant) cells.

β-catenin; 

PubMed: 24165128     


HeLa cells were treated with 5 μm CHIR-99021 for the indicated times. The samples were analyzed by Western blot.

30709379 23993102 24165128
Growth inhibition assay
Cell viability; 

PubMed: 22039301     


HMC-1.2 cells were cultured in the absence or presence of GSK3β inhibitor (CHIR 99021, 3–30 µM) and viability cell count was assessed by trypan blue dye exclusion after 24 h, 48 h or 72 h. All data are presented as the mean ± SEM of three independent experiments conducted in triplicate. p < 0.05*, p < 0.001** p < 0.0001*** vs vehicle treated control cells.

22039301
Immunofluorescence
SOX10 / EdU; 

PubMed: 28174705     


Incorporation of EdU (white arrowheads) into SOX10+ ENS (enteric nervous system) cells with and without CHIR-99021.

Oct3/4; 

PubMed: 24779365     


Fluorescence micrographs showing the protein expression of Brachyury/Oct3/4 after a three day differentiation of ES-D3 cells with BIO (0.5 μM), SB-216763 (5 μM), CHIR-99021 (5 μM), or CHIR-98014 (1 μM). Nuclei were counterstained with DAPI (blue). The merge is shown in yellow. Original magnification 400x. Scale bar = 50 μm.

28174705 24779365
In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. [1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. [4]

Protocol

Kinase Assay:

[1]

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Cell-free kinase assays:

Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research:

[3]

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  • Cell lines: INS-1E
  • Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM
  • Incubation Time: 1, or 4 days
  • Method:

    Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes
  • Dosages: ~48 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (185.33 mM)
Water Insoluble
Ethanol '2 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
24mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 501.8
Formula

C22H18Cl2N8.HCl

CAS No. 1797989-42-4
Storage powder
in solvent
Synonyms N/A
Smiles CC1=CN=C(N1)C2=CN=C(N=C2C3=C(C=C(C=C3)Cl)Cl)NCCNC4=NC=C(C=C4)C#N.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?

  • Answer:

    The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.

  • Question 2:

    How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?

  • Answer:

    Please change the culture medium each day to add the new comound in it if the duration is more than 72h.

  • Question 3:

    What is the difference between S2924 and S1263? Which one is better for differentiation?

  • Answer:

    S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID