CHIR-99021 (CT99021) HCl

Catalog No.S2924

CHIR-99021 (CT99021) HCl Chemical Structure

Molecular Weight(MW): 501.8

CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.

Size Price Stock Quantity  
In DMSO USD 340 In stock
USD 90 In stock
USD 170 In stock
USD 570 In stock
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Cited by 73 Publications

9 Customer Reviews

  • Differentiation induced by treatment with the Gsk3 inhibitor in hPSCs is β-catenin dependent. H9-7TGP cells were treated with 12 uM CH in mTeSR1 for 4 d. Immunofluorescent staining for Oct4, Isl1, and Nkx2.5 was compared with GFP expression. (Scale bars, 50 uM.)

    Proc Natl Acad Sci U S A 2012 109(27):E1848-57. CHIR-99021 (CT99021) HCl purchased from Selleck.

    Gpc4 regulates ESC response to Wnt/β-catenin signaling.  (E) Quantitative analysis of control and Gpc4-mutant beating foci exposed to the GSK3 inhibitor, CHIR 99021 (1 and 3 μM, from day 0 to day 5). Analysis was done at different time points. Gpc4- mutant cells treated with CHIR 99021 behave as controls (mean±SEM; n=2).

    Stem Cells 2012 30, 1863-1874. CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

    Characterization of rES cells. A: image of normal rES cell colonies on feeder layers. B: rES colonies were positive for AP staining. CeE: rES colonies readily expressed pluripotent markers, Sox2 (C), Oct4 (D), and SSEA-1 (E). Blue, DAPI. Scale bars: 100 um.

    J Genet Genomics 2012 39, 643e651. CHIR-99021 (CT99021) HCl purchased from Selleck.

    Inhibition of GSK3 activity induces an autophagic response in human pancreatic cancer cells. PANC1 cells were treated for 24 h with DMSO, CHIR99021 (CHIR; 5 uM) or the mTOR inhibitor Torin1 (250 nM). Autophagosome (punctate LC3B) (D) and lysosome (E) labeling was visualized with a Zeiss LSM700 confocal microscope. Images were acquired with a 20x (D) or 63x (E) objective. Nuclei were stained with DAPI. Scale bars, 20 祄.

    J Biol Chem 2015 290(9), 5592-605 . CHIR-99021 (CT99021) HCl purchased from Selleck.

  • A. Vero cells were pretreated with the indicated concentrations of CHIR-99021 for 1 h and then infected with PEDV (MOI = 1) for 8 or 10 h. Cell lysates were prepared and analyzed using Western blotting.

    PLoS One, 2016, 11(8):e0161508. CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    Smad linker phosphorylation involves GSK3 in human melanoma lines. Inhibition of Smad2 and Smad3 linker phosphorylation in the presence of the specific GSK3 specific inhibitor, CT99021. 24 hours post seeding, WM793 and 1205LU cells were serum-starved for about 16 hours, and incubated in the absence (2) or presence (+) of 2 uM of CT99021 for two hours. Immunoblots were performed as in A. p: Phosphorylated. S: Serine; T: Threonine.

    PLoS One 2012 7, e47312. CHIR-99021 (CT99021) HCl purchased from Selleck.

  • SKOV3/DDP cells were treated with a range of concentration of (A) CHIR-99021 or in combination with 20 µM cisplatin for 48 h.

    Exp Ther Med, 2016, 12(6):3851-3858. CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    PANC1 cells were treated with GSK3 inhibitors SB216763 (SB 10µM or SB 20µM) or CHIR99021 (CHIR 5µM) for 72h.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

     Activity of canonical WNT/ β -CATENIN signals induced by CHIR 99021 in the developing human lung. Human lung tissue explants obtained at 15 weeks of gestation were exposed to 0, 5 and 10 μM CHIR 99021 in vitro for 72 h. (A-B) Western blot analysis showed expression of β -CATENIN in human lung tissues. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (C) qRT-PCR analysis detected a dose-dependent increase in WNT signaling transcription factors and target genes following exposure to CHIR 99021. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (D-K) Histology examination showed obvious morphogenetic changes of the airway epithelial cells associated with CHIR 99021 treated lung tubes. Scale bars: D-G =100 μm; H-K = 20 μm.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. [1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). [2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
3T3-L1 NEj1eZlHfW6ldHnvckBCe3OjeR?= NGTrSnc{KM7:TR?= NHjjSXQ1KGh? NHnlSGZFVVOR NIXGWVRKdmirYnn0d{BxemWjZHnwc4N6fGViZHnm[oVz\W62aXH0bY9v NF75XIMyOjB3NUKwNC=>
insulin receptor–expressing CHO-IR M1vUOmZ2dmO2aX;uJGF{e2G7 M3O5TFUxOCCwTR?= NXLqSHJnOzBibXnu M3TxWGROW09? MXHBZ5RqfmG2ZYOgS4x6[2:pZX6gV5lvfGijc3WgLGdUMQ>? M1vHXVEzPjB4NEm3
H4IIE M2HT[WZ2dmO2aX;uJGF{e2G7 MonUNVAh|ryP NWDNdYJxOjRiaB?= MmjFSG1UVw>? NHTVfI1T\We3bHH0[ZMh|rJvY3H0[Y5qdiCjY4Tpeol1gSCjbnSgWGlTTSClb370ZYlvcW6pIIDyc41wfGW{IHHjeIl3cXS7 NUjKd4J{OTV|NUCxPVU>
Oct4/Sox2/Klf4-infected MEFs MXPGeY5kfGmxbjDBd5NigQ>? MonFNVAh|ryP NX\jfWg{OjhiZB?= MXnEUXNQ MkXWVJJwdW:2ZYOgUWVHeyC{ZYDyc4dz[W2vaX7nJHRz[W6|ZIXj[YQh[nliT3P0OEwhW2:6MjygZY5lKEuuZkS= MXyxPVg{QTB3NR?=
Sca-1+ HPC M2XaS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2LhNVIh|ryP MnvMOkBl M2rGb2ROW09? M122TnN6dmW{Z3nzeIlk[WyueTDwdo9ud3SnczDTZ4EuOSwEoFjQR{Bxem:uaX\ldoF1cW:wIHHu[EBkd2yxbomg[o9zdWG2aX;uJJRw\2W2aHXyJJdqfGhiRVfG Mn:xNlE3OjR|NEi=
HMC1.2 Mm\US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUe3UngxOzBizszN MYC3NkBp MYLEUXNQ M4f0fHJm\HWlZYOgZ4VtdCC|dYL2bZZidA>? MoiyNlIxOzl|MEG=
Endothelial cell(EC) NHTXcHJHfW6ldHnvckBCe3OjeR?= NXfLU5NLOyEQvF2= NGj3S2RFVVOR MVfQdo9ud3SnczDlcoRwfGinbHnhcEBk\WyuIDjFR{kh\GmoZnXy[Y51cWG2aX;u NXPTfIRTOjJyNUKwNFY>
PANC1 MYLBdI9xfG:|aYOgRZN{[Xl? NYHvUYZWyqB3IN88US=> MXy3NkBp MmjCSG1UVw>? NIfYNZBVemmpZ3Xyd{BidiCjcH;weI91cWNicnXzdI9ve2V? NWLTe|Z7OjJ{MEGxPFY>
INS-1E MUPGeY5kfGmxbjDBd5NigQ>? NFHCNIIzNjVizszN MljxNlQhcA>? M171XWROW09? MoLiTY5lfWOnczDhJJJm\HWldHnvckBw\iCQUEGgc5ZmemW6cILld5Nqd25? Mlv1NlI1Ojd5MES=
hiPSC-NPs NV6yTYVCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVPaU2NjO+LCid88US=> M17XXFczKGh? MnTVSG1UVw>? MmXTTY5kemWjc3XzJINmdGxicILvcIln\XKjdHnvcuKh M1nFOlIzPjR{Nki3
platelets NFS5d3dHfW6ldHnvckBCe3OjeR?= MYWxNEDPxE1? MnPvNVUhdWmw M2DBb2ROW09? M1rYd2lv[3KnYYPld{B1cHKxbXLpck1u\WSrYYTl[EBxdGG2ZXzleEBi\2e{ZXfheIlwdiCjbnSgRXRRKHOnY4LleIlwdg>? Mo\mNlMzOzl6N{e=
DN3 NWP6XJVETnWwY4Tpc44hSXO|YYm= NFnpOmUyOCEQvF2= NWG4XpBDQTZiaB?= M3HVemROW09? NUPTV2xoUW6lcnXhd4V{KGOnbHyg[IV3\WyxcH3lcpQh[W6mIIDyc4xq\mW{YYTpc44> M365XFI{PTJ4OUi5
embryonic stem cells (ESC) NWjmTWJHTnWwY4Tpc44hSXO|YYm= MVKzJOK2VQ>? NVHGdoVUOTFiZB?= NID2WnFFVVOR MnW2VJJwdW:2ZYOgd4Vt\i2{ZX7le4FtKG:oIH7hx893\S22eYDlJI1wfXOnIHXtZpJ6d26rYzDzeIVuKGOnbHzzJEhGW0Nr MnH3NlM5PDN|MUK=
H460 NFG1XJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYG1JOK2VQ>? M{m1SVI1KGh? NETlWGVFVVOR NXj3dFhjWHKxbX;0[ZMh[2WubDDwdo9tcW[ncnH0bY9v NWjCXndSOjR|Nke2PFg>
H157 M2TN[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LvflUhyrWP MVKyOEBp MY\EUXNQ Mkj0VJJwdW:2ZYOgZ4VtdCCycn;sbYZmemG2aX;u MYmyOFM3PzZ6OB?=
H460 MVvBdI9xfG:|aYOgRZN{[Xl? MV61JOK2VQ>? NEHLXGYzPCCq M4HmV2ROW09? NUnuepNzSXS2ZX71ZZRmeyClZXzsJIFxd3C2b4Ppdy=> M{X3TFI1OzZ5Nki4
H157 MkWyRZBweHSxc3nzJGF{e2G7 Ml\EOUDDvU1? NUjYblFbOjRiaB?= NUjS[5NuTE2VTx?= M{H2TmRw\XNibn;0JJNq\26rZnnjZY51dHliYX\m[YN1KGGyb4D0c5Nqew>? NWrSU2JWOjR|Nke2PFg>
A549 M4DyS2Fxd3C2b4Ppd{BCe3OjeR?= NVzpS3hxPSEEtV2= M3TGfVI1KGh? NWj2TY9DTE2VTx?= NXTKS2dKUW6lcnXhd4V{KGOnbHygZZBweHSxc3nz M4[zW|I1OzZ5Nki4
Neural progenitor cells MXfGeY5kfGmxbjDBd5NigQ>? NX\1U4xiOyEQvF2= NF:5UmYyOCCm NUTLVZp1TE2VTx?= NFWwToRKdmS3Y3XzJIdmdmW{YYTpc44hd2ZibnX1doFtKHC{b3flcol1d3JiY3XscJM> NUXBTHRrOjR4M{iwN|Q>
ES-D3 NFzTXY5EgXSxdH;4bYMhSXO|YYm= M4fjfFExyqEQvF2= NVHQb5ZEOyCm NFLF[|JFVVOR M4TnZmlv\HWlZYOgcY9l\XKjdHWgZ5l1d3SxeHnjbZR6KHerdHigTWM2OCCxZjC0Mlkh|ryP NHHVNYMzPDd5OUO2OS=>
ES-CCE NWLxb|dMS3m2b4TvfIlkKEG|c3H5 M3TzV|ExyqEQvF2= MXWzJIQ> M4TOfWROW09? MnTjTY5lfWOnczDtc4RmemG2ZTDjfZRwfG:6aXPpeJk> NX;0bW4yOjR5N{mzOlU>
ES-D3 MVzGeY5kfGmxbjDBd5NigQ>? M4PuWlXDqM7:TR?= NWnvWphxOyCm MXHEUXNQ NH;lNotUfHKxbnfsfUBqdmS3Y3XzJJRp\SCjY4Tpeol1gSCxZjDXcpQw[mW2YT3jZZRmdmmwIIDheIh4[Xl? MYGyOFc4QTN4NR?=
ES-CCE M2j0U2Z2dmO2aX;uJGF{e2G7 NYPOOIdoPcLizszN MXqzJIQ> Ml7RSG1UVw>? NVnh[IdyW3S{b37ncJkhcW6mdXPld{B1cGViYXP0bZZqfHlib3[gW451N2KndHGtZ4F1\W6rbjDwZZRpf2G7 Mki0NlQ4Pzl|NkW=
SW982 NYrpRWVlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2G4OWlEPTB;MD6yNVQyPiEQvF2= MYnTRW5ITVJ?
GCT MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LFPGlEPTB;MD6zNFY4OiEQvF2= NIOwfZpUSU6JRWK=
769-P NW\KT|dvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDVVmw2UUN3ME2wMlU5PDl7IN88US=> M4PTenNCVkeHUh?=
LOXIMVI Mom4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\wS5dKSzVyPUCuOVg5OzJizszN M{\JRXNCVkeHUh?=
D-336MG M1vZO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\PTWM2OD1yLki0OlIh|ryP NH;KW2xUSU6JRWK=
S-117 MoP2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mof6TWM2OD1yLkmyNVMyKM7:TR?= NIraT25USU6JRWK=
D-247MG M1vmOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVv0U|Q4UUN3ME2wMlk1QDV{IN88US=> MmLGV2FPT0WU
TYK-nu NGf3emJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4HaNWlEPTB;MT6wO|kyOiEQvF2= NWXIXY85W0GQR1XS
A498 NVT0fldpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF3kPWRKSzVyPUGuN|A1PjlizszN MnzMV2FPT0WU
BCPAP NE\jPVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1rsVmlEPTB;MT62Olg6QCEQvF2= MYfTRW5ITVJ?
KYSE-180 M2jJdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHWZXFKSzVyPUGuO|I4QDhizszN NXvVXlM3W0GQR1XS
MIA-PaCa-2 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTFwN{W5O|ch|ryP NUDM[JF1W0GQR1XS
HCC1395 M4mwOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTFwN{iyO|Mh|ryP NEi4TZlUSU6JRWK=
RS4-11 NXzPTolTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDqTmc4UUN3ME2xMlk2PDF3IN88US=> Ml3mV2FPT0WU
NCI-H2122 NHTmZ45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFuzOohKSzVyPUKuNlc3QDRizszN MXfTRW5ITVJ?
SNU-423 M{\XRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmf2TWM2OD1{LkS5O|g4KM7:TR?= NXPIWGZTW0GQR1XS
SK-LMS-1 NX3aeFVuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIHLSpdKSzVyPUKuOlQyOjhizszN M4PMO3NCVkeHUh?=
RPMI-7951 NWHKVGpbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXPCeJNuUUN3ME2yMlcxOTl|IN88US=> NG\LN4hUSU6JRWK=
COLO-829 Ml[3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXYUFNMUUN3ME2yMlc3PTRizszN M{nSe3NCVkeHUh?=
SCC-9 NFHQZppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTJwOUW0PFUh|ryP MVvTRW5ITVJ?
A704 NHLxXXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXTRNVZIUUN3ME2zMlA5OTJizszN NWjjUpFJW0GQR1XS
HOP-62 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIjrUFFKSzVyPUOuN|YxPTRizszN M1;xUXNCVkeHUh?=
HCC1569 NWDMRnhYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTNwNEizNFUh|ryP NGLveXRUSU6JRWK=
A172 NUfBZ2FuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3SOpZKUUN3ME2zMlc2PDl6IN88US=> MX7TRW5ITVJ?
MOLT-16 NFHYU4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTNwOEi1OVYh|ryP NGjIU4RUSU6JRWK=
TE-15 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTNwOUWzOFMh|ryP NFrUZYdUSU6JRWK=
OE19 M2rwOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\DbYJKSzVyPUOuPVY1PTFizszN MoHCV2FPT0WU
MHH-ES-1 NFKyPFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF31NYJKSzVyPUSuNFQ2PDVizszN NVXkOZpkW0GQR1XS
NKM-1 MmfmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1LkfmlEPTB;ND6xN|k4OSEQvF2= NVnRT3Y1W0GQR1XS
RCC10RGB MmPtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3n4WmlEPTB;ND6yPVQ4QSEQvF2= MkDTV2FPT0WU
BxPC-3 M2TWV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmCyTWM2OD12LkewN|kyKM7:TR?= NHPCUIdUSU6JRWK=
ALL-PO NHK1O2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkTjTWM2OD12LkizPFU5KM7:TR?= NWTtRnhnW0GQR1XS
SK-OV-3 Mk[zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nK[WlEPTB;ND65PVM1KM7:TR?= M3PKZ3NCVkeHUh?=
SW1710 M2C5OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTVwNEK0OVYh|ryP NV7kWoNQW0GQR1XS
NCI-H2452 MkH0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLyTWM2OD13LkS1OlI5KM7:TR?= MVPTRW5ITVJ?
D-392MG NUnmdVdyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn\GTWM2OD13LkS5NVQ2KM7:TR?= Mmj0V2FPT0WU
LB2518-MEL M2nUWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLNcWU4UUN3ME21MlczPjJ5IN88US=> M1\US3NCVkeHUh?=
BL-41 M{LNeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTVwOEGyOlYh|ryP MUTTRW5ITVJ?
WM-115 Mnn2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2TUSmlEPTB;NT64NVUxQSEQvF2= NWq5SZM6W0GQR1XS
SK-MEL-2 NXXqUo92T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEG3VlJKSzVyPU[uN|g{PDRizszN NWXwTo9HW0GQR1XS
CGTH-W-1 NGDsXWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTTXnlxUUN3ME22MlQyPyEQvF2= NXH3OIZOW0GQR1XS
BB65-RCC MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\BdGlEPTB;Nj61N|E6PSEQvF2= Mn23V2FPT0WU
CAL-85-1 MmS5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWPkRnJSUUN3ME22MlYxQTN4IN88US=> MXvTRW5ITVJ?
RT-112 NF3xbYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTdwMU[0OVYh|ryP NIrOR5dUSU6JRWK=
MC116 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzaPFFKSzVyPUeuNlg3QTFizszN Mo\aV2FPT0WU
G-361 M17QdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3joNmlEPTB;Nz60OlU3OSEQvF2= MUHTRW5ITVJ?
SW1417 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWnJR|UxRTdwNkS1PFQh|ryP NFvRU3lUSU6JRWK=
MV-4-11 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fjVmlEPTB;OD61OFMxPCEQvF2= MmnVV2FPT0WU
CAL-72 M1XMXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\sNWlEPTB;OD63OlEyOyEQvF2= NWPZUFB{W0GQR1XS
MKN28 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWnJR|UxRThwOEi1NVYh|ryP NETPc21USU6JRWK=
MEG-01 NWPpd2NjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHfsR5FKSzVyPUiuPVkxPDNizszN MXrTRW5ITVJ?
ACHN NHnNfmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M12zcmlEPTB;OT6zNFU1PiEQvF2= MWjTRW5ITVJ?
NCI-H1755 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DnO2lEPTB;OT6zPVk5PiEQvF2= M3LYZ3NCVkeHUh?=
NCI-H64 NITMXJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXjc4tKSzVyPUmuOFIzPTVizszN MV\TRW5ITVJ?
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MFH-ino M1zUfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3TGcWlEPTB;MUOxMlEyPCEQvF2= M{\xWHNCVkeHUh?=
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BB30-HNC M3TiWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV\LR3VoUUN3ME2xN|QvOTF4IN88US=> MnzPV2FPT0WU
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Detroit562 NVWxPHE2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4r0e2lEPTB;MUSxMlQ3PiEQvF2= M3z2R3NCVkeHUh?=
OCI-AML2 M3HBUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjBV2hrUUN3ME2xOFEvPTB5IN88US=> M4LZTnNCVkeHUh?=
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LB647-SCLC MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXzMNFZNUUN3ME2xOFgvPTZ{IN88US=> NGPDZ|JUSU6JRWK=
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TE-9 NVLiflVST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M170ZmlEPTB;MUS5MlQ1OyEQvF2= NV;VTY9[W0GQR1XS
Caov-3 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1fVd2lEPTB;MUS5MlcyPCEQvF2= MVXTRW5ITVJ?
L-540 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWnNc4tIUUN3ME2xOFkvPzVizszN NEnmdohUSU6JRWK=
NCI-H748 NFHEdZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEPwfJpKSzVyPUG1NE4zODlizszN MnzrV2FPT0WU
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AsPC-1 NWjuTFdST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV[5TpFPUUN3ME2xOVIvPzZ2IN88US=> Mlr2V2FPT0WU
MDA-MB-361 M1nlUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2LRUmlEPTB;MUWzMlE3PCEQvF2= M2PuWHNCVkeHUh?=
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Calu-1 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn71TWM2OD1zNUSuNFA{KM7:TR?= MojnV2FPT0WU
RERF-LC-MS M1HhUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPQTnJMUUN3ME2xOVQvPzd{IN88US=> NUDjc|V7W0GQR1XS
DK-MG NEj5elZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoGwTWM2OD1zNUWuNVg{KM7:TR?= M{HKcnNCVkeHUh?=
CAL-120 NIjMO3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPQZ5NxUUN3ME2xOVUvQDJzIN88US=> MX;TRW5ITVJ?
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NCI-H2052 NET1fGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI\XeYlKSzVyPUG1PU42QDNizszN MYTTRW5ITVJ?
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CAL-62 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYPObo9zUUN3ME2xOlIvPjF|IN88US=> NV\uSJQ3W0GQR1XS
CHP-212 NXPKNY1RT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH65[IlKSzVyPUG2N{43OjZizszN Mn7DV2FPT0WU
AU565 MoDUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFjmOphKSzVyPUG2N{43PCEQvF2= NWrzTJU1W0GQR1XS
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Calu-3 NEG0[m1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGf3U4lKSzVyPUG2PE4{OTFizszN MW\TRW5ITVJ?
CP66-MEL M33DVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfXUpRKSzVyPUG2PE41OTVizszN MYjTRW5ITVJ?
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LB2241-RCC NWDtZm9lT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHLEbHBKSzVyPUG4OU4yPDVizszN NHnhXHVUSU6JRWK=
NCI-H1563 NU\KfYZvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\sWVJSUUN3ME2xPFUvOjB7IN88US=> NEj1U2RUSU6JRWK=
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SW780 MmPPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWTMNWVQUUN3ME2xPVYvPzN2IN88US=> MnnjV2FPT0WU
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JVM-3 NYHZc5h2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWnYcWVIUUN3ME2xPVgvPDF7IN88US=> M1XXenNCVkeHUh?=
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QIMR-WIL MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVT3ZoUxUUN3ME2yNFAvOTF4IN88US=> NVP2PHJuW0GQR1XS
SJRH30 MmjoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWfsPGdUUUN3ME2yNFAvPTF3IN88US=> NFP2WFRUSU6JRWK=
COLO-679 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{LMbWlEPTB;MkCwMlg2QSEQvF2= NF3kRoFUSU6JRWK=
MDA-MB-231 MlfGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnz4TWM2OD1{MECuPVAzKM7:TR?= M13uZnNCVkeHUh?=
MFM-223 Mn2zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTJyMT61PFch|ryP NH;mZo1USU6JRWK=
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SK-MEL-24 NYnPW2x2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;SdZo5UUN3ME2zNlQvOTFzIN88US=> NHnMVHdUSU6JRWK=
NTERA-S-cl-D1 Ml;RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHiSoxWUUN3ME2zNlYvPDh7IN88US=> MXPTRW5ITVJ?
SK-MEL-30 MofnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYnJR|UxRTN{Nj63N|gh|ryP MY\TRW5ITVJ?
LS-123 MknnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzUVpFvUUN3ME2zNlgvOTZ2IN88US=> M1\kXHNCVkeHUh?=
SF268 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYj2cXVYUUN3ME2zN|AvOjZ3IN88US=> MU\TRW5ITVJ?
BT-474 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTN|Mj60NVgh|ryP NGjqN5VUSU6JRWK=
NCI-H1522 NVPwb3J6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYriWZBDUUN3ME2zN|QvOzN7IN88US=> MYPTRW5ITVJ?
LC-2-ad M2i1Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3KwS2lEPTB;M{O2MlU6OSEQvF2= NWLa[2pUW0GQR1XS
LAN-6 NEX4NolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTN|Nj65O|ch|ryP MkfYV2FPT0WU
ARH-77 NWfaOHNET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTN|Nz6zNkDPxE1? MnnhV2FPT0WU
HuO9 M32ySWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2DzPGlEPTB;M{O3MlQ4KM7:TR?= NV;hcHQ1W0GQR1XS
MLMA MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWGwSmxvUUN3ME2zN|gvOTV3IN88US=> Mnz5V2FPT0WU
Raji MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnoW3dGUUN3ME2zN|gvOzhizszN NX7OPHpWW0GQR1XS
GT3TKB MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;WT|ZKSzVyPUOzPE41OTJizszN MlX2V2FPT0WU
NCI-H441 NHjENFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkTsTWM2OD1|NEGuOFE5KM7:TR?= MXrTRW5ITVJ?
NCI-H2030 NEX2XIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{LGS2lEPTB;M{SxMlYxQSEQvF2= M33LV3NCVkeHUh?=
VM-CUB-1 M{nCOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEWye2JKSzVyPUO0Nk43OzZizszN MkjJV2FPT0WU
KP-4 MmfKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmWxTWM2OD1|NEOuO|M{KM7:TR?= M4\0OXNCVkeHUh?=
PF-382 NUjyeGVST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoDOTWM2OD1|NESuOFIzKM7:TR?= NXrrUINmW0GQR1XS
NB13 MnTiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlPTTWM2OD1|NEWuOVgh|ryP NWe1TlJZW0GQR1XS
D-502MG M1\tXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPEWYFRUUN3ME2zOFUvQDF{IN88US=> MWnTRW5ITVJ?
CAL-54 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zwfWlEPTB;M{S1Mlg4QSEQvF2= MoTxV2FPT0WU
DOHH-2 M1\HeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUPJR|UxRTN2Nj62OUDPxE1? NH7Ed2hUSU6JRWK=
SK-PN-DW NWPkbpllT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGPZSo5KSzVyPUO0O{41OjZizszN NV;3R5U4W0GQR1XS
NCI-H720 NIDkUpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoTyTWM2OD1|NEmuPFQ3KM7:TR?= Mmf1V2FPT0WU
SW1990 NFLyU|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRTN3MT6yPEDPxE1? NEn0TYlUSU6JRWK=
NCI-H2196 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLz[nRKSzVyPUO1Nk43OjRizszN NEfDTlhUSU6JRWK=
P30-OHK MoLsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTN3Mz6wOlMh|ryP M3HyVHNCVkeHUh?=
NCI-H358 M2PN[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M13hWWlEPTB;M{W1MlM2PiEQvF2= MUnTRW5ITVJ?
CW-2 NH3rSoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3XhWmlEPTB;M{W2MlUzQSEQvF2= M{HjbXNCVkeHUh?=
COLO-678 M{\JV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTN3Nz64OlMh|ryP Mk\xV2FPT0WU
NCI-H2126 NWHiU3h1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4j0dmlEPTB;M{W4MlUzOSEQvF2= MmG1V2FPT0WU
DMS-53 NFe3cYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NED1[oNKSzVyPUO1PE44OTFizszN NH\1fJhUSU6JRWK=
NCI-H1838 M2e5Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2r2ZWlEPTB;M{W5Mlk5PyEQvF2= MWXTRW5ITVJ?
SJSA-1 NFTQTmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVrJR|UxRTN4MD65N|kh|ryP MlK2V2FPT0WU
ChaGo-K-1 M1;Se2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTN4Mz65NlQh|ryP NF;QNpRUSU6JRWK=
IST-SL2 Ml3DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jsUGlEPTB;M{eyMlkzPiEQvF2= MnjUV2FPT0WU
U-266 M4DuOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2X6NmlEPTB;M{ezMlE{OiEQvF2= MXrTRW5ITVJ?
P31-FUJ MoTwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWTET4c5UUN3ME2zO|YvQTZ2IN88US=> NIW1dFBUSU6JRWK=
HCC2157 NHG0VHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrtTVBKSzVyPUO3PE4yPjFizszN Mn7wV2FPT0WU
COR-L88 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnTiTWM2OD1|OEOuOlc4KM7:TR?= NE\vTlVUSU6JRWK=
NCI-H596 MoDrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHTZpNKSzVyPUO4OU4{PCEQvF2= NYrySoY3W0GQR1XS
GOTO NHLtbo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHQTWM2OD1|OUCuOlQyKM7:TR?= NVq1eIxvW0GQR1XS
NCI-H719 NXjRRW5[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVTsdmxPUUN3ME2zPVEvQDl|IN88US=> NU\FbWRqW0GQR1XS
MN-60 NGXpWJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTN7Mz65OFgh|ryP MVXTRW5ITVJ?
IST-SL1 NIrxXnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDjTWM2OD1|OUSuOFA3KM7:TR?= Mn3wV2FPT0WU
TE-6 M3;p[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHPMeFlKSzVyPUO5O{4zPTRizszN NUjjemNPW0GQR1XS
SW962 NGLGcWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTjR3J1UUN3ME2zPVgvPjlizszN MnO1V2FPT0WU
FADU M1vTO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGLQcGdKSzVyPUSwN{4xQDNizszN MUfTRW5ITVJ?
ECC4 M1rx[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHq4VpNKSzVyPUSwPE41PThizszN NV3IUVZIW0GQR1XS
MES-SA MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfSdJRKSzVyPUSwPU46QCEQvF2= M3f6S3NCVkeHUh?=
NCI-H716 NHPxb3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTRzMD6wO|Uh|ryP M2frPXNCVkeHUh?=
GR-ST MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnTTWM2OD12MUCuOFk4KM7:TR?= M3nacXNCVkeHUh?=
KY821 NVrzTIE5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvpfXpKSzVyPUSxOE4xQDZizszN M{TVW3NCVkeHUh?=
NCI-H2141 NI[5OJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4fsNWlEPTB;NEK0MlM5QCEQvF2= MWfTRW5ITVJ?
MDA-MB-134-VI NXXTXoJUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXLJR|UxRTR|MT6wN|Mh|ryP M4[wUXNCVkeHUh?=
RH-1 NX[5dWJtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUXJR|UxRTR|NT6wPUDPxE1? MWrTRW5ITVJ?
AM-38 NI[xXnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmXLTWM2OD12M{iuOVQh|ryP MlL3V2FPT0WU
BL-70 NE[0SWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWCzNJBIUUN3ME20N|kvPDJ7IN88US=> NGK4[JlUSU6JRWK=
COLO-684 M1XoUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGXlUGtKSzVyPUS0PE45QDhizszN NI\o[IlUSU6JRWK=
DMS-79 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmHnTWM2OD12NEmuOlgh|ryP M3TaWnNCVkeHUh?=
EW-1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjoe5RuUUN3ME20OVIvQDh6IN88US=> M4XrT3NCVkeHUh?=
MS-1 NES1TGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3znNWlEPTB;NEW0MlM5PSEQvF2= NIf4b|hUSU6JRWK=
A4-Fuk M3;WcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzITWM2OD12NUSuOVA4KM7:TR?= NUHNeXEyW0GQR1XS
TALL-1 NI\xZnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2DkPGlEPTB;NEW1MlI6PyEQvF2= MX3TRW5ITVJ?
SCLC-21H NWTHV3luT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnnqTWM2OD12NUWuOlk6KM7:TR?= NFjOcGhUSU6JRWK=
KMS-12-PE NUX2O4NHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH3wT4VKSzVyPUS1Ok45OjJizszN MnTMV2FPT0WU
TE-441-T M3vRNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIXsbYRKSzVyPUS1O{4yQDZizszN NInpbVlUSU6JRWK=
A3-KAW MlzNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXhSmdKSzVyPUS2NE45PDFizszN NFv0dYJUSU6JRWK=
NCI-H1092 MnKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rRXmlEPTB;NE[zMlk1OyEQvF2= M3TVN3NCVkeHUh?=
COR-L279 NH7iS21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjCUXFKSzVyPUS2OE45PDdizszN M1rpNHNCVkeHUh?=
NCI-SNU-5 NWLuXmpLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7JSVZjUUN3ME20O|IvOjhizszN NEDHPItUSU6JRWK=
JAR M4fPcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYDLfoI{UUN3ME20O|QvPzh6IN88US=> MXPTRW5ITVJ?
NCI-H446 NUL0c|hUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2CyfmlEPTB;NEe3MlA5KM7:TR?= MVzTRW5ITVJ?
HDLM-2 NGm1SGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvuTWM2OD12OECuPFY6KM7:TR?= NYDNNJNiW0GQR1XS
D-283MED MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M13MfWlEPTB;NEi4MlkzPCEQvF2= M{LkZ3NCVkeHUh?=
NCI-H1395 MmDoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\DSW93UUN3ME20PFkvODB2IN88US=> NX7jfZA4W0GQR1XS
RPMI-6666 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mm\BTWM2OD12OUGuN|k5KM7:TR?= NVPj[4RWW0GQR1XS
CPC-N Ml\5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoG0TWM2OD13MEKuNFY{KM7:TR?= NHeycFZUSU6JRWK=
EC-GI-10 NHnEd49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTVyMj6xOlkh|ryP MWTTRW5ITVJ?
CA46 NF;1ZppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmHQTWM2OD13MESuOFA4KM7:TR?= NXXLdFZXW0GQR1XS
EHEB M1Pzb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnQTWM2OD13MEeuNlA5KM7:TR?= NGPTWlFUSU6JRWK=
RPMI-8402 MnO0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXrJR|UxRTVzMD6wNlEh|ryP Ml7JV2FPT0WU
DU-4475 MmC1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFnIcpFKSzVyPUWxNU4yODFizszN NVmxV4RRW0GQR1XS
P12-ICHIKAWA MmDMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnrxTWM2OD13MUGuOFE3KM7:TR?= NWHYR5NCW0GQR1XS
NCI-H1694 Mo\CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXm4VGtJUUN3ME21NVIvPDN7IN88US=> NHz0d2RUSU6JRWK=
NCI-H2171 MmTVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmTMTWM2OD13MUKuO|A2KM7:TR?= MnrPV2FPT0WU
Mo-T M2[zVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXINWVKSzVyPUWxOU4yOTZizszN M1\EWnNCVkeHUh?=
HL-60 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPVZYlyUUN3ME21NlIvQTJ2IN88US=> MnWxV2FPT0WU
NCI-H1436 NFPsXGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoPHTWM2OD13MkOuNFY1KM7:TR?= MVPTRW5ITVJ?
NCI-H510A M4HMfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRTV{ND62PVYh|ryP NUHsWXZMW0GQR1XS
NCI-H2081 NFjqPGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF7jdpZKSzVyPUWyOU42OTNizszN NF7MVlJUSU6JRWK=
NB5 MnSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NID5U5dKSzVyPUWyOk41OyEQvF2= MYHTRW5ITVJ?
NCI-H2227 NELiTGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;GblZkUUN3ME21N|AvOjV2IN88US=> M4\nWHNCVkeHUh?=
HSC-4 Mn\FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3K[Wc1UUN3ME21N|kvOjl|IN88US=> M1Tje3NCVkeHUh?=
RL NXjKT29[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEfjUZNKSzVyPUW0Nk41PDJizszN MYLTRW5ITVJ?
EVSA-T NEPibXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXPOdVU3UUN3ME21OFMvPjh2IN88US=> NFzQe|JUSU6JRWK=
NCI-H889 M1X6Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXmWFBoUUN3ME21OFcvOjNizszN NXmzXZBYW0GQR1XS
COLO-320-HSR M4DvcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;hN2lEPTB;NUWxMlM2KM7:TR?= MXrTRW5ITVJ?
EW-11 NUD6[JN[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;uTWM2OD13NUKuN|k6KM7:TR?= MXnTRW5ITVJ?
UACC-812 NGSxSHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3foXWlEPTB;NUW5MlM6OSEQvF2= M1zwTnNCVkeHUh?=
CAL-148 NYj1WoxoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXjJR|UxRTV4NT6wPFkh|ryP M3T0N3NCVkeHUh?=
K052 M3TESWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1XFfGlEPTB;NUe5MlIyOyEQvF2= MkTPV2FPT0WU
L-428 NEDqNHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;6WIpuUUN3ME22NFAvPjdzIN88US=> M2rLd3NCVkeHUh?=
LP-1 MojpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTZyMz61PFch|ryP MXXTRW5ITVJ?
ML-2 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1;pUmlEPTB;NkC2MlU4QSEQvF2= M16yfnNCVkeHUh?=
KARPAS-45 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnTjTWM2OD14MUiuNlIyKM7:TR?= NXHJO3o1W0GQR1XS
HT MkGwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvPO25KSzVyPU[yNk44QDVizszN NYTDSIFPW0GQR1XS
NCI-H226 NXzMRphkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLWNIlLUUN3ME22NlgvOzV|IN88US=> MnH3V2FPT0WU
L-363 Mli4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\qd3hKSzVyPU[0PE4xPjRizszN MYjTRW5ITVJ?
IM-9 NUjHV3JRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHv5T5JKSzVyPU[1NU42PDFizszN NIXCTZdUSU6JRWK=
TUR NE\rOI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTZ5MT6zNlIh|ryP NFrYZlFUSU6JRWK=
NCI-SNU-16 NVHQd4ZrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXafFVIUUN3ME22PFAvPDZ{IN88US=> NI[w[ndUSU6JRWK=
NCI-SNU-1 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGHtXIFKSzVyPU[5NE4yPzJizszN NHrZTYlUSU6JRWK=
U-87-MG M4rvOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;FSZBqUUN3ME22PVQvPTh3IN88US=> MkPUV2FPT0WU
KM-H2 NHT0R|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVK4UHV3UUN3ME22PVkvODV7IN88US=> NGjJXXhUSU6JRWK=
DEL MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmL2TWM2OD15MECuPFM5KM7:TR?= M4OzfnNCVkeHUh?=
KARPAS-422 M{TQWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHrD[|JKSzVyPUe4PU4{OTVizszN M17TbXNCVkeHUh?=
BC-3 NFLw[ZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXrqcW1lUUN3ME24NFUvOjB|IN88US=> NF3Le4ZUSU6JRWK=
NCI-H1155 NGr0cGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TxeWlEPTB;OEG4MlA2QSEQvF2= NHrreo5USU6JRWK=
NCI-H1581 MkXhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTlyMD6yN|Eh|ryP NX7m[YlNW0GQR1XS

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In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. [1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. [4]

Protocol

Kinase Assay:

[1]

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Cell-free kinase assays:

Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research:

[3]

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  • Cell lines: INS-1E
  • Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM
  • Incubation Time: 1, or 4 days
  • Method:

    Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes
  • Formulation: Formulated as solutions in 20 mM citrate-buffered 15% Captisol or as fine suspensions in 0.5% carboxymethylcellulose.
  • Dosages: ~48 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (185.33 mM)
Ethanol 2 mg/mL (3.98 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
24mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 501.8
Formula

C22H18Cl2N8.HCl

CAS No. 1797989-42-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?

  • Answer:

    The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.

  • Question 2:

    How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?

  • Answer:

    Please change the culture medium each day to add the new comound in it if the duration is more than 72h.

  • Question 3:

    What is the difference between S2924 and S1263? Which one is better for differentiation?

  • Answer:

    S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

GSK-3 Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID