CHIR-99021 (CT99021) HCl

Catalog No.S2924

CHIR-99021 (CT99021) HCl Chemical Structure

Molecular Weight(MW): 501.8

CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.

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Cited by 80 Publications

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. [1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). [2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
3T3-L1 MmLtSpVv[3Srb36gRZN{[Xl? Ml35N{DPxE1? M2G3PVQhcA>? MnPTSG1UVw>? M4SxU2lvcGmkaYTzJJBz\WGmaYDvZ5l1\SCmaX\m[ZJmdnSrYYTpc44> NEOxWXUyOjB3NUKwNC=>
insulin receptor–expressing CHO-IR M1PabmZ2dmO2aX;uJGF{e2G7 NIiwOGs2ODBibl2= MlnHN|AhdWmw NG\hTlFFVVOR NIjLbHNC[3SrdnH0[ZMhT2y7Y3;n[Y4hW3mwdHjhd4UhMEeVKR?= NULDNoU5OTJ4ME[0PVc>
H4IIE MmPoSpVv[3Srb36gRZN{[Xl? MXmxNEDPxE1? M1PRU|I1KGh? MnW1SG1UVw>? M{X5PXJm\3WuYYTld{DPui2lYYTlcolvKGGldHn2bZR6KGGwZDDUTXJGKGOxboThbY5qdmdicILvcY91\XJiYXP0bZZqfHl? NWq1PYZGOTV|NUCxPVU>
Oct4/Sox2/Klf4-infected MEFs NFPXVFFHfW6ldHnvckBCe3OjeR?= M4nX[|ExKM7:TR?= Ml7XNlgh\A>? MX3EUXNQ NI\YbY5Rem:vb4Tld{BOTU[|IILldJJw\3KjbX3pcochXHKjboPkeYNm\CCkeTDPZ5Q1NCCVb4iyMEBidmRiS3zmOC=> NHLoTGIyQTh|OUC1OS=>
Sca-1+ HPC M1fQTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX6yJO69VQ>? MnH2OkBl MXfEUXNQ MmnHV5lv\XKpaYP0bYNidGy7IIDyc41wfGW|IGPjZU0yM8LiSGDDJJBzd2yrZnXyZZRqd25iYX7kJINwdG:weTDmc5Ju[XSrb36geI9o\XSqZYKge4l1cCCHR1[= MVmyNVYzPDN2OB?=
HMC1.2 M3;WZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWGzNEDPxE1? M4\zclczKGh? NYDRSoN7TE2VTx?= M3;sPXJm\HWlZYOgZ4VtdCC|dYL2bZZidA>? MUGyNlA{QTNyMR?=
Endothelial cell(EC) MUnGeY5kfGmxbjDBd5NigQ>? NX3EZmdCOyEQvF2= M4XLXGROW09? NWDKeI1rWHKxbX;0[ZMh\W6mb4To[Yxq[WxiY3XscEApTUNrIHTp[oZmemWwdHnheIlwdg>? NIPxdlMzOjB3MkCwOi=>
PANC1 NIfO[2VCeG:ydH;zbZMhSXO|YYm= MYRCpFUh|ryP NWT2eVBGPzJiaB?= MXXEUXNQ M3TBfXRzcWepZYLzJIFvKGGyb4D0c5Rq[yC{ZYPwc45{\Q>? M2DySFIzOjBzMUi2
INS-1E NXn2[XZJTnWwY4Tpc44hSXO|YYm= MVeyMlUh|ryP NIL4N|YzPCCq MkP1SG1UVw>? MmTmTY5lfWOnczDhJJJm\HWldHnvckBw\iCQUEGgc5ZmemW6cILld5Nqd25? NVHzXVNVOjJ2Mke3NFQ>
hiPSC-NPs MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH3IblE{6oDLzszN MnTCO|IhcA>? NX;nTYRGTE2VTx?= MXPJcoNz\WG|ZYOgZ4VtdCCycn;sbYZmemG2aX;uxsA> M2HTTVIzPjR{Nki3
platelets MXrGeY5kfGmxbjDBd5NigQ>? NHHJ[|AyOCEQvF2= MWqxOUBucW5? NFfvZ5VFVVOR NHL5clFKdmO{ZXHz[ZMhfGi{b33ibY4udWWmaXH0[YQheGyjdHXs[ZQh[WepcnXnZZRqd25iYX7kJGFVWCC|ZXPy[ZRqd25? MXyyN|I{QTh5Nx?=
DN3 M1zFfGZ2dmO2aX;uJGF{e2G7 MV[xNEDPxE1? M2DDR|k3KGh? MoP0SG1UVw>? MWjJcoNz\WG|ZYOgZ4VtdCCmZY\lcI9xdWWwdDDhcoQheHKxbHnm[ZJifGmxbh?= M13pZVI{PTJ4OUi5
embryonic stem cells (ESC) NYDWW3NlTnWwY4Tpc44hSXO|YYm= M4Hqd|MhyrWP M2S0V|EyKGR? NF7FeFNFVVOR M4XLcnBzd22xdHXzJJNmdGZvcnXu[ZdidCCxZjDuZeOwfmVvdInw[UBud3W|ZTDlcYJzgW:waXOgd5RmdSClZXzsd{ApTVOFKR?= MoT6NlM5PDN|MUK=
H460 NXzmNZJ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHq[pI4PSEEtV2= M3S5eVI1KGh? Mn\CSG1UVw>? M4jpTnBzd22xdHXzJINmdGxicILvcIln\XKjdHnvci=> M3X1UFI1OzZ5Nki4
H157 Ml[5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;pXpJ4PSEEtV2= MX:yOEBp MWDEUXNQ Mn7LVJJwdW:2ZYOgZ4VtdCCycn;sbYZmemG2aX;u NEj0UGgzPDN4N{[4PC=>
H460 MVvBdI9xfG:|aYOgRZN{[Xl? NHnYUVU2KML3TR?= MnLvNlQhcA>? MlT0SG1UVw>? M4roXmF1fGWwdXH0[ZMh[2WubDDhdI9xfG:|aYO= M2[0SlI1OzZ5Nki4
H157 MUPBdI9xfG:|aYOgRZN{[Xl? MWW1JOK2VQ>? MXSyOEBp MX7EUXNQ MVnEc4V{KG6xdDDzbYdvcW[rY3HueIx6KGGoZnXjeEBieG:ydH;zbZM> MVyyOFM3PzZ6OB?=
A549 M2HiUGFxd3C2b4Ppd{BCe3OjeR?= NEPnPIg2KML3TR?= NFjCdWkzPCCq NFX3c5NFVVOR MmH1TY5kemWjc3XzJINmdGxiYYDvdJRwe2m| NVLlVJUyOjR|Nke2PFg>
Neural progenitor cells NFPRUFVHfW6ldHnvckBCe3OjeR?= MVWzJO69VQ>? M3nVSFExKGR? M2rIU2ROW09? MVfJcoR2[2W|IHflcoVz[XSrb36gc4YhdmW3cnHsJJBzd2enbnn0c5Ih[2WubIO= M3K2WFI1PjN6MEO0
ES-D3 Mlz4R5l1d3SxeHnjJGF{e2G7 NIjqbJkyOMLizszN M17SfFMh\A>? M1viS2ROW09? MYfJcoR2[2W|IH3v[IVz[XSnIHP5eI91d3irY3n0fUB4cXSqIFnDOVAhd2ZiND65JO69VQ>? NUTDSIRQOjR5N{mzOlU>
ES-CCE NVnrfW55S3m2b4TvfIlkKEG|c3H5 MmDSNVDDqM7:TR?= MUCzJIQ> MlH1SG1UVw>? M3X0d2lv\HWlZYOgcY9l\XKjdHWgZ5l1d3SxeHnjbZR6 NGLXT3czPDd5OUO2OS=>
ES-D3 MVXGeY5kfGmxbjDBd5NigQ>? NFvrXo42yqEQvF2= M4n2SVMh\A>? MmTaSG1UVw>? MmDRV5Rzd26pbImgbY5lfWOnczD0bIUh[WO2aY\peJkhd2ZiV370M4JmfGFvY3H0[Y5qdiCyYYToe4F6 NYLLXY5jOjR5N{mzOlU>
ES-CCE MX\GeY5kfGmxbjDBd5NigQ>? NU\LUlNPPcLizszN M{LuW|Mh\A>? NIXHVpNFVVOR MoDQV5Rzd26pbImgbY5lfWOnczD0bIUh[WO2aY\peJkhd2ZiV370M4JmfGFvY3H0[Y5qdiCyYYToe4F6 MWqyOFc4QTN4NR?=
SW982 M{Xr[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW\JR|UxRTBwMkG0NVYh|ryP NVzqfpNPW0GQR1XS
GCT NXrCenkyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4XFRmlEPTB;MD6zNFY4OiEQvF2= MVvTRW5ITVJ?
769-P MnPyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTBwNUi0PVkh|ryP NVzZbIFHW0GQR1XS
LOXIMVI NVnnVZlIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{fjOWlEPTB;MD61PFg{OiEQvF2= NV3IWZZCW0GQR1XS
D-336MG MnL0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3JWYY4UUN3ME2wMlg1PjJizszN NE[w[5ZUSU6JRWK=
S-117 NI\xcoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mkm0TWM2OD1yLkmyNVMyKM7:TR?= M{f5WXNCVkeHUh?=
D-247MG MmjaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHTTmlKSzVyPUCuPVQ5PTJizszN NFjWUoFUSU6JRWK=
TYK-nu M17re2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXJTWM2OD1zLkC3PVEzKM7:TR?= Mly2V2FPT0WU
A498 NXP5RZFPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHS1ZVJKSzVyPUGuN|A1PjlizszN MliyV2FPT0WU
BCPAP NV3rPZlJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2fyPWlEPTB;MT62Olg6QCEQvF2= MlL6V2FPT0WU
KYSE-180 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEH6OoJKSzVyPUGuO|I4QDhizszN M37DPXNCVkeHUh?=
MIA-PaCa-2 NXHjVpdsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml\zTWM2OD1zLke1PVc4KM7:TR?= Ml2zV2FPT0WU
HCC1395 NX3wdnlTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjTW2lKSzVyPUGuO|gzPzNizszN M3fMOHNCVkeHUh?=
RS4-11 MnzqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIDDRXFKSzVyPUGuPVU1OTVizszN NXT1bVlzW0GQR1XS
NCI-H2122 NXrW[I9lT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3vDS2lEPTB;Mj6yO|Y5PCEQvF2= Mn3oV2FPT0WU
SNU-423 NX;MZWJ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUfpcnl5UUN3ME2yMlQ6Pzh5IN88US=> M3XWc3NCVkeHUh?=
SK-LMS-1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTJwNkSxNlgh|ryP MXvTRW5ITVJ?
RPMI-7951 NIn1SYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGS2R21KSzVyPUKuO|AyQTNizszN MlLMV2FPT0WU
COLO-829 MoP6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3[ySmlEPTB;Mj63OlU1KM7:TR?= NXK3TmFrW0GQR1XS
SCC-9 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDNTWM2OD1{Lkm1OFg2KM7:TR?= MUjTRW5ITVJ?
A704 M3TrbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXrJR|UxRTNwMEixNkDPxE1? M3jpdnNCVkeHUh?=
HOP-62 M3vqTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2KxUmlEPTB;Mz6zOlA2PCEQvF2= MoWwV2FPT0WU
HCC1569 NFjCWGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvIUGRIUUN3ME2zMlQ5OzB3IN88US=> NHvnbWRUSU6JRWK=
A172 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTNwN{W0PVgh|ryP MVHTRW5ITVJ?
MOLT-16 NWHBbYZ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnrITWM2OD1|Lki4OVU3KM7:TR?= Mki5V2FPT0WU
TE-15 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTNwOUWzOFMh|ryP NIPYWlNUSU6JRWK=
OE19 NH\ReHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHXWRoVKSzVyPUOuPVY1PTFizszN MUfTRW5ITVJ?
MHH-ES-1 M{PaSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFK1S2xKSzVyPUSuNFQ2PDVizszN NInOd5NUSU6JRWK=
NKM-1 MmTDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFftdmxKSzVyPUSuNVM6PzFizszN MmLzV2FPT0WU
RCC10RGB M4\Kb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLCbZBKSzVyPUSuNlk1PzlizszN MoHqV2FPT0WU
BxPC-3 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLNTWM2OD12LkewN|kyKM7:TR?= MXnTRW5ITVJ?
ALL-PO Mo\oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;0fpJ2UUN3ME20Mlg{QDV6IN88US=> M3fBT3NCVkeHUh?=
SK-OV-3 M3TCU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXLTWM2OD12Lkm5N|Qh|ryP M{jsb3NCVkeHUh?=
SW1710 MnX4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{fIWmlEPTB;NT60NlQ2PiEQvF2= Ml:4V2FPT0WU
NCI-H2452 NXviSZNoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mny5TWM2OD13LkS1OlI5KM7:TR?= MoLXV2FPT0WU
D-392MG MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHnmO3NKSzVyPUWuOFkyPDVizszN MV7TRW5ITVJ?
LB2518-MEL Mk\WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTVwN{K2Nlch|ryP MUXTRW5ITVJ?
BL-41 NX34T2RiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XwdWlEPTB;NT64NVI3PiEQvF2= NWjxXXFRW0GQR1XS
WM-115 M4KwbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlG4TWM2OD13LkixOVA6KM7:TR?= M2S1NnNCVkeHUh?=
SK-MEL-2 MlzPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTZwM{izOFQh|ryP MmjQV2FPT0WU
CGTH-W-1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfYXoI2UUN3ME22MlQyPyEQvF2= NF3WXm9USU6JRWK=
BB65-RCC MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHn6[WFKSzVyPU[uOVMyQTVizszN MoTmV2FPT0WU
CAL-85-1 M2DmeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnf3TWM2OD14Lk[wPVM3KM7:TR?= MYHTRW5ITVJ?
RT-112 NGfFbnBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33UO2lEPTB;Nz6xOlQ2PiEQvF2= MnfXV2FPT0WU
MC116 NHTNWZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NULlOINDUUN3ME23MlI5PjlzIN88US=> NVjPOnVYW0GQR1XS
G-361 NHrpb|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfOXZhqUUN3ME23MlQ3PTZzIN88US=> MYLTRW5ITVJ?
SW1417 NIDud4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTdwNkS1PFQh|ryP NHX6WXJUSU6JRWK=
MV-4-11 NFP2d5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;WTWM2OD16LkW0N|A1KM7:TR?= M4\4eHNCVkeHUh?=
CAL-72 NHriT3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRThwN{[xNVMh|ryP NGnvNW5USU6JRWK=
MKN28 NF3SNYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{POdmlEPTB;OD64PFUyPiEQvF2= MXXTRW5ITVJ?
MEG-01 MlfQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXzBTmZ2UUN3ME24Mlk6ODR|IN88US=> NX\XXlNoW0GQR1XS
ACHN NGTuSlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn3lTWM2OD17LkOwOVQ3KM7:TR?= NX7Ecnk3W0GQR1XS
NCI-H1755 M2DvZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFjke3pKSzVyPUmuN|k6QDZizszN MXvTRW5ITVJ?
NCI-H64 MmTpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;0TWM2OD17LkSyNlU2KM7:TR?= Mnj4V2FPT0WU
COLO-205 M2fWc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHLPZpNKSzVyPUmuOFM5OjFizszN Ml35V2FPT0WU
CTV-1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFjUN3NKSzVyPUmuOFM5QTRizszN NHKxSpRUSU6JRWK=
U-2-OS NGjX[5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1jYPWlEPTB;OT60OVAxPiEQvF2= NYi2OJBOW0GQR1XS
LB996-RCC M2jKbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnPqTWM2OD17LkWyN|k4KM7:TR?= Ml7VV2FPT0WU
SK-N-DZ NFTL[W9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPFTWM2OD17Lk[1O|QzKM7:TR?= M1HU[XNCVkeHUh?=
EFM-19 M1jH[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVPJR|UxRTlwN{W1OlYh|ryP MmXCV2FPT0WU
DBTRG-05MG M1\RSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4HqTGlEPTB;OT63O|c4KM7:TR?= NHPhWGdUSU6JRWK=
U-118-MG NYDVO5lLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTlwOUS0OFQh|ryP M3y1O3NCVkeHUh?=
LB1047-RCC NWjke|V4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEGycGtKSzVyPUmuPVk4QThizszN MnPrV2FPT0WU
NCI-H28 NEHKeZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFHSVJFKSzVyPUGwMlQ1OTNizszN M4raRXNCVkeHUh?=
MZ2-MEL Mkn4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGXMVVhKSzVyPUGwMlQ3PzdizszN MVnTRW5ITVJ?
M059J NEfYUJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXP1SllUUUN3ME2xNE43OTB{IN88US=> NYHCWYp5W0GQR1XS
HT-3 NVrUUVZuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlrMTWM2OD1zMD62NVc6KM7:TR?= NVe3[|RFW0GQR1XS
SNU-387 M1f4N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVLJR|UxRTFyLkeyN|Uh|ryP NIfGR4pUSU6JRWK=
NCI-H526 NIPtWG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1vybGlEPTB;MUGuNVM3PSEQvF2= M{SwcnNCVkeHUh?=
HEL M2\PZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTFzLk[1O|kh|ryP NUiyeWdLW0GQR1XS
PA-1 MnTtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTFzLkmzJO69VQ>? NFfpXHNUSU6JRWK=
PC-14 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjOTWM2OD1zMj6zNlQh|ryP MV7TRW5ITVJ?
C8166 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEjnOYpKSzVyPUGzMlI{OThizszN NGnBcpBUSU6JRWK=
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RPMI-8866 NFrXcJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\yTWM2OD13Nz6yOFEzKM7:TR?= NFP2OGxUSU6JRWK=
BV-173 NIHpTWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH7udZdKSzVyPUW3MlUyPDJizszN NHLmUoVUSU6JRWK=
LCLC-97TM1 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTV7LkC1OVMh|ryP MV\TRW5ITVJ?
HT-1376 NHvLflBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NID3TINKSzVyPUW5MlA6OjFizszN M1vSUnNCVkeHUh?=
ACN NWG2WW41T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWra[Y5xUUN3ME21PU4yPTh5IN88US=> NELLTVhUSU6JRWK=
LU-134-A M4LiRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVjPSZBKUUN3ME21PU43ODF4IN88US=> MoXxV2FPT0WU
SiHa MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\NTWM2OD13OT62OFg4KM7:TR?= MkmwV2FPT0WU
EKVX MmDES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mmr0TWM2OD14MT60PVU5KM7:TR?= MkHMV2FPT0WU
NMC-G1 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXHTWM2OD14MT65NlQzKM7:TR?= MkS1V2FPT0WU
NCI-H1734 NIfObopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX7JR|UxRTZzLkm2OlMh|ryP MmD6V2FPT0WU
K-562 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTZ{LkK0OVIh|ryP M2\VTnNCVkeHUh?=
T-24 NVX5W21OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTZ{LkexNlYh|ryP NE\LeJFUSU6JRWK=
SCH M4Wyfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4LrUWlEPTB;NkSuNlM2OSEQvF2= MXnTRW5ITVJ?
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MFE-296 M1;0d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHvwbZFKSzVyPU[0MlQ1PzNizszN MmDIV2FPT0WU
HuCCT1 NGn0SGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrN[5lKSzVyPU[3MlA{PzRizszN M3G4cHNCVkeHUh?=
NCI-H1703 NYXmT5o2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLNdlcyUUN3ME22PE4yOTdzIN88US=> MXLTRW5ITVJ?
C-4-II NXXKT2lvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjzTWM2OD14OT6zOlA4KM7:TR?= M3zuNXNCVkeHUh?=
NCI-H2342 M3fzUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4G5fmlEPTB;NkmuN|k3OSEQvF2= MmLMV2FPT0WU
Hs-578-T NIHRTlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;qe2Z7UUN3ME23NE4{KM7:TR?= M1PscnNCVkeHUh?=
HCC1806 M3TGdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTdzLkS1PFch|ryP NWjvcFRiW0GQR1XS
EW-16 M1\XXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTdzLke0NVUh|ryP NVTPS5BpW0GQR1XS
KALS-1 NITkR3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPGTWM2OD15Mz61OFc2KM7:TR?= NVPzN5FnW0GQR1XS
RT4 NFvQOpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTd|LkW3OFIh|ryP M{Hpc3NCVkeHUh?=
LS-411N M3fyTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUfJR|UxRTd2LkK1Nlgh|ryP Mk[3V2FPT0WU
SK-MEL-1 M4PwSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkL1TWM2OD15NT64NFE6KM7:TR?= M33wfnNCVkeHUh?=
8-MG-BA MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvMTWM2OD15Nj6xNFk3KM7:TR?= MkezV2FPT0WU
HSC-3 MkmxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3;PbWlEPTB;N{euNFI5OiEQvF2= MlHyV2FPT0WU
NB6 NVm0WZpoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGK1NWFKSzVyPUe4MlEzQTlizszN NVvmZWZQW0GQR1XS
PANC-03-27 NEDMb3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX3CcJBVUUN3ME23PE41OzVizszN NY[3XnZwW0GQR1XS
KP-N-YS M2nQfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1S2W2lEPTB;N{iuOVg4QCEQvF2= MVHTRW5ITVJ?
SF295 NEHHSJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUf1fZlqUUN3ME23PU46PTV|IN88US=> NW\nOGhCW0GQR1XS
HCE-T NIDuVIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TObmlEPTB;OECuNVU5QCEQvF2= MY\TRW5ITVJ?
KYSE-410 NHmwd4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRThyLkK4OVch|ryP NW\pZZM6W0GQR1XS
NB12 MorXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRThyLkmzOlkh|ryP MVvTRW5ITVJ?
NCI-H661 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVzhUppXUUN3ME24NU4{PDRizszN NFTSfJRUSU6JRWK=
NCI-H1417 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2jJemlEPTB;OEGuOFM3QCEQvF2= NUDkd3hZW0GQR1XS
KMOE-2 NIm4R29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnMO21KSzVyPUixMlg2OzFizszN NXXWXohtW0GQR1XS
SW1573 MmnBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHBbW5bUUN3ME24Nk42QDl|IN88US=> MWfTRW5ITVJ?
BFTC-905 MkTES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTh2LkCxO|Ih|ryP M2fVbnNCVkeHUh?=
SBC-1 NYPEfHRMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1y1UGlEPTB;OESuNFIxQCEQvF2= NIj4R5NUSU6JRWK=
MDA-MB-175-VII NHfFcZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\NTWM2OD16NT63OFI3KM7:TR?= MVfTRW5ITVJ?
TE-12 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoP5TWM2OD16OT6zO|QyKM7:TR?= MonJV2FPT0WU
NCI-H650 NVO0[|J3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LxSGlEPTB;OEmuPFczPCEQvF2= MlXJV2FPT0WU
C-33-A M1vMRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHWeHJKSzVyPUmwMlU1KM7:TR?= NVnje4F7W0GQR1XS
MZ7-mel MlTuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmXiTWM2OD17MT6yOlc3KM7:TR?= MXnTRW5ITVJ?
BOKU NWjUN21YT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2PjRmlEPTB;OUKuOlk2PyEQvF2= NGHSXmxUSU6JRWK=
CAL-33 M1zGbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV21NWwyUUN3ME25N{4xPjBzIN88US=> MULTRW5ITVJ?
23132-87 Mn:2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjpTWM2OD17Mz6xOlgh|ryP M1u3TXNCVkeHUh?=
J82 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRTl|Lke4NVEh|ryP NWraXYlxW0GQR1XS
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MEL-JUSO NEHZc4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXjJR|UxRTl2Lkm1OEDPxE1? NIjvc4NUSU6JRWK=
RMG-I NHjES4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELhUJJKSzVyPUm1MlExPzJizszN MmnyV2FPT0WU
A2058 NEjTeFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkmyTWM2OD17NT60OlE4KM7:TR?= MWHTRW5ITVJ?
Caov-4 M17IWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrvTWM2OD17NT62PFUh|ryP MoDjV2FPT0WU
OS-RC-2 MmnhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DTOWlEPTB;OUeuOFYyKM7:TR?= M1nQR3NCVkeHUh?=
MKN7 NFSyR2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXLSmhzUUN3ME25O{44PDN7IN88US=> M1LiZXNCVkeHUh?=
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PC-3 M4\ybWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljnTWM2OD17OT6wOVY1KM7:TR?= M3z6bnNCVkeHUh?=
NCI-H292 NULJcmlJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;DcGlEPTB;OUmuN|AxPCEQvF2= Ml7hV2FPT0WU
IGR-1 NEPPUXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLDTWM2OD17OT6zNVE6KM7:TR?= MVPTRW5ITVJ?
Mewo Mo\kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTl7LkW2NFMh|ryP MlLoV2FPT0WU
LU-65 NWrwfmZuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1i2O2lEPTB;MUCxMlA5OSEQvF2= NIGzUFVUSU6JRWK=
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TE-11 NYPmNZZkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrHTWM2OD1zMEGuO|I1KM7:TR?= NHfqOZdUSU6JRWK=
SK-MES-1 NGH5[5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTFyMT63OlIh|ryP NVTJNZg3W0GQR1XS
KE-37 M4[yU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mne0TWM2OD1zMEKuPVUh|ryP NUKxN5RnW0GQR1XS
MKN45 NGrlTWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG[zVGlKSzVyPUGwN{42QDRizszN NFP2[lBUSU6JRWK=
UM-UC-3 NHvSUGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn3pTWM2OD1zMEOuPFQzKM7:TR?= MYHTRW5ITVJ?
SNU-C2B NWf1TY1zT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7XVodKSzVyPUGwOU4xOjNizszN NHjQSG9USU6JRWK=
RPMI-8226 NHXFTllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorNTWM2OD1zME[uOlc5KM7:TR?= MnL2V2FPT0WU
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NCI-H23 NWHpS5hRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;ITWM2OD1zMEiuN|U4KM7:TR?= MULTRW5ITVJ?
HOP-92 MnG4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1PVWWlEPTB;MUC4MlQ4PSEQvF2= NWf4NohjW0GQR1XS
FTC-133 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2LhVGlEPTB;MUC4MlQ5QCEQvF2= NXzFZXNLW0GQR1XS
D-263MG NF3FNlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTFyOD65OlEh|ryP MlTqV2FPT0WU
DU-145 Mn7ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU\JR|UxRTFyOT65OFUh|ryP Ml7mV2FPT0WU
A253 NXniPHRmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVjkfI1kUUN3ME2xNVAvPDd7IN88US=> MnvOV2FPT0WU
HCT-15 NH[1WnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWjJR|UxRTFzMD62OlEh|ryP NFjyUoVUSU6JRWK=
WSU-NHL NF\kcVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILlcIVKSzVyPUGxNk4xOSEQvF2= NXL6c3VMW0GQR1XS
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MDA-MB-157 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlX3TWM2OD1zMUKuOFkh|ryP Mk\SV2FPT0WU
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SIG-M5 NIXq[WZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M173TGlEPTB;MUGzMlEzPSEQvF2= M{j0[XNCVkeHUh?=
786-0 NFX0W|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEmyZnlKSzVyPUGxN{41PTNizszN MoPRV2FPT0WU
DMS-273 NUT1V45wT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGPGbFBKSzVyPUGxOU43OTdizszN MojwV2FPT0WU
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OVCAR-8 Mm\5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTFzNz62Olgh|ryP M4XqdXNCVkeHUh?=
ATN-1 NX2xbW8yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzUVIVKSzVyPUGxO{45OjhizszN NF\VUZBUSU6JRWK=
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SKG-IIIa NFrxUYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRTFzOD6xNVYh|ryP M3XCbHNCVkeHUh?=
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OVCAR-3 NF\BN3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2LoXGlEPTB;MUG5MlkyPSEQvF2= NXe4bHNoW0GQR1XS
NCI-N87 M1LXWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;NTWM2OD1zMkGuNFQ1KM7:TR?= NF3xcWpUSU6JRWK=
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HAL-01 MmD2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTF{Mj60PVkh|ryP M{\PRXNCVkeHUh?=
HT-29 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfjRll3UUN3ME2xNlIvPzd6IN88US=> NU\aUIgyW0GQR1XS
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SW1463 MnfVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGDCPFdKSzVyPUGyOE4xPzdizszN MonIV2FPT0WU
NCI-H2087 MnjqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTF{ND6yPFMh|ryP MnPSV2FPT0WU
UMC-11 NIn4PYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\VfGlEPTB;MUK2MlA{QCEQvF2= M2riRnNCVkeHUh?=
EB-3 NFuxTZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXftd2x3UUN3ME2xNlcvODF|IN88US=> NV;PcVAyW0GQR1XS
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VMRC-RCZ MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWnJR|UxRTF|MD6zO|Uh|ryP NVX1UI9OW0GQR1XS
MFH-ino NVL5cm5JT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVTJR|UxRTF|MT6xNVQh|ryP Mnj2V2FPT0WU
HT-1080 NVLiblRyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTF|Mj6yNFQh|ryP MlztV2FPT0WU
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Capan-2 NFyyTlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRTF|Mz65O|Uh|ryP MVvTRW5ITVJ?
BB30-HNC NUf6WJp{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlTaTWM2OD1zM{SuNVE3KM7:TR?= MmLqV2FPT0WU
CP50-MEL-B M{fmO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTF|ND6yJO69VQ>? NIXGS|BUSU6JRWK=
JEG-3 NEnwR4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn7iTWM2OD1zM{SuN|U6KM7:TR?= MXLTRW5ITVJ?
NCI-H1437 NGf3SWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI\BcIhKSzVyPUGzOE42QSEQvF2= M4CzZXNCVkeHUh?=
NOS-1 M1j0VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlzoTWM2OD1zM{SuO|Y2KM7:TR?= MnqzV2FPT0WU
G-402 NU[zPW1LT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjUTWM2OD1zM{WuNlk2KM7:TR?= Ml2zV2FPT0WU
OAW-42 M{f2NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1m0c2lEPTB;MUO3Mlg2PiEQvF2= MnuxV2FPT0WU
Ca-Ski M{nQVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XUc2lEPTB;MUO4Mlg{OSEQvF2= NV;sPFR[W0GQR1XS
BHT-101 NVywWWJ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjnOGZKSzVyPUG0NE4zODhizszN M1;ze3NCVkeHUh?=
OVCAR-4 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\4SVR7UUN3ME2xOFAvPzR7IN88US=> M{TTenNCVkeHUh?=
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DOHH-2 M1S5R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYnJR|UxRTN2Nj62OUDPxE1? Mo\6V2FPT0WU
SK-PN-DW M3z3UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV\lSHptUUN3ME2zOFcvPDJ4IN88US=> NYXQXIVxW0GQR1XS
NCI-H720 M1vXfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTN2OT64OFYh|ryP M4jJR3NCVkeHUh?=
SW1990 NHjxUXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3KxR2lEPTB;M{WxMlI5KM7:TR?= M4PO[XNCVkeHUh?=
NCI-H2196 NHnIcVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTnTWM2OD1|NUKuOlI1KM7:TR?= M3\IenNCVkeHUh?=
P30-OHK MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPUbXNKSzVyPUO1N{4xPjNizszN NWD1TWlGW0GQR1XS
NCI-H358 NHzDeYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3LJW2lEPTB;M{W1MlM2PiEQvF2= M{nhd3NCVkeHUh?=
CW-2 NUPuUXcyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGDqN4VKSzVyPUO1Ok42OjlizszN M2rrSXNCVkeHUh?=
COLO-678 MnLzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3uxbGlEPTB;M{W3Mlg3OyEQvF2= MkfOV2FPT0WU
NCI-H2126 Mnu2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFzaR4VKSzVyPUO1PE42OjFizszN Ml3QV2FPT0WU
DMS-53 M1TaXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRTN3OD63NVEh|ryP NXvQSIxvW0GQR1XS
NCI-H1838 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXnPZhKSzVyPUO1PU46QDdizszN NELPeWNUSU6JRWK=
SJSA-1 MoTHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTN4MD65N|kh|ryP MlHoV2FPT0WU
ChaGo-K-1 M3\vU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1XZdGlEPTB;M{[zMlkzPCEQvF2= NVrsemRbW0GQR1XS
IST-SL2 M13XPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M17pWmlEPTB;M{eyMlkzPiEQvF2= NXXJbFc{W0GQR1XS
U-266 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2i4WWlEPTB;M{ezMlE{OiEQvF2= NUfZOm82W0GQR1XS
P31-FUJ M2r6cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIO2VVVKSzVyPUO3Ok46PjRizszN NVfKNZZEW0GQR1XS
HCC2157 MmnUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVT1fFh2UUN3ME2zO|gvOTZzIN88US=> NXfuSYZkW0GQR1XS
COR-L88 NX3reZRTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYftZ5VKUUN3ME2zPFMvPjd5IN88US=> NGjJUnVUSU6JRWK=
NCI-H596 NFq4RllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTN6NT6zOEDPxE1? NY\nXlJ2W0GQR1XS
GOTO NV\iSpVXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvyc3J6UUN3ME2zPVAvPjRzIN88US=> NGK4dFlUSU6JRWK=
NCI-H719 NELYR5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPjd2JRUUN3ME2zPVEvQDl|IN88US=> NVrSOZZZW0GQR1XS
MN-60 MmHSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTN7Mz65OFgh|ryP M{DtRnNCVkeHUh?=
IST-SL1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXzJR|UxRTN7ND60NFYh|ryP NXTEWWt7W0GQR1XS
TE-6 MkCzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHxTWM2OD1|OUeuNlU1KM7:TR?= NVHSWIJoW0GQR1XS
SW962 M1zVS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGrqcpRKSzVyPUO5PE43QSEQvF2= M{LkSHNCVkeHUh?=
FADU NX\aPFdnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTRyMz6wPFMh|ryP NFfVZoJUSU6JRWK=
ECC4 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTyTWM2OD12MEiuOFU5KM7:TR?= NXTadlZZW0GQR1XS
MES-SA M2rkO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn3VTWM2OD12MEmuPVgh|ryP MojzV2FPT0WU
NCI-H716 NW\FT|NpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmL6TWM2OD12MUCuNFc2KM7:TR?= M13DPXNCVkeHUh?=
GR-ST MkD3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTRzMD60PVch|ryP NHrGfIxUSU6JRWK=
KY821 NGXyWVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVrJR|UxRTRzND6wPFYh|ryP NFSw[nRUSU6JRWK=
NCI-H2141 NV\ZRVJ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXzJR|UxRTR{ND6zPFgh|ryP MVXTRW5ITVJ?
MDA-MB-134-VI NXz3Rm1uT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnOyTWM2OD12M{GuNFM{KM7:TR?= NW\VXldPW0GQR1XS
RH-1 MlGwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGL4OpRKSzVyPUSzOU4xQSEQvF2= MULTRW5ITVJ?
AM-38 M4DQVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkDYTWM2OD12M{iuOVQh|ryP MonoV2FPT0WU
BL-70 NGXXUGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|UxRTR|OT60Nlkh|ryP MlTMV2FPT0WU
COLO-684 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2rrV2lEPTB;NES4Mlg5QCEQvF2= M1PuWXNCVkeHUh?=
DMS-79 NGnNRplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37nNmlEPTB;NES5MlY5KM7:TR?= MlG0V2FPT0WU
EW-1 NIfmUYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUHJR|UxRTR3Mj64PFgh|ryP NIHSPVNUSU6JRWK=
MS-1 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTR3ND6zPFUh|ryP M1vYdHNCVkeHUh?=
A4-Fuk MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4HvU2lEPTB;NEW0MlUxPyEQvF2= MWPTRW5ITVJ?
TALL-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlGyTWM2OD12NUWuNlk4KM7:TR?= MX3TRW5ITVJ?
SCLC-21H NH[3T|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF;GUnJKSzVyPUS1OU43QTlizszN NG\6UnBUSU6JRWK=
KMS-12-PE NHfvVmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHuT|J2UUN3ME20OVYvQDJ{IN88US=> MX;TRW5ITVJ?
TE-441-T NUfJeIhVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTR3Nz6xPFYh|ryP NEHrfWVUSU6JRWK=
A3-KAW NG\iRo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzvTWM2OD12NkCuPFQyKM7:TR?= NEK0T|VUSU6JRWK=
NCI-H1092 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{j1V2lEPTB;NE[zMlk1OyEQvF2= MlX2V2FPT0WU
COR-L279 NGfJWWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXH3cFE5UUN3ME20OlQvQDR5IN88US=> M3iwPHNCVkeHUh?=
NCI-SNU-5 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkH3TWM2OD12N{KuNlgh|ryP MYTTRW5ITVJ?
JAR MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTR5ND63PFgh|ryP MW\TRW5ITVJ?
NCI-H446 M2G0Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTR5Nz6wPEDPxE1? MXLTRW5ITVJ?
HDLM-2 Mke5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2r0cWlEPTB;NEiwMlg3QSEQvF2= M1TqbnNCVkeHUh?=
D-283MED M{jt[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIHqNXNKSzVyPUS4PE46OjRizszN NYrWW5pRW0GQR1XS
NCI-H1395 NEnhNW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3nm[mlEPTB;NEi5MlAxPCEQvF2= NXHpbXB4W0GQR1XS
RPMI-6666 NH[xVpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTuXpdGUUN3ME20PVEvOzl6IN88US=> NVXsTWNxW0GQR1XS
CPC-N NFHzS49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XIcGlEPTB;NUCyMlA3OyEQvF2= M3XJZnNCVkeHUh?=
EC-GI-10 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3WxbmlEPTB;NUCyMlE3QSEQvF2= M1PLbnNCVkeHUh?=
CA46 NXO3N5k1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mlv5TWM2OD13MESuOFA4KM7:TR?= M4rkcXNCVkeHUh?=
EHEB MorkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHsTWM2OD13MEeuNlA5KM7:TR?= M3nUbXNCVkeHUh?=
RPMI-8402 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;YbXVyUUN3ME21NVAvODJzIN88US=> MnXWV2FPT0WU
DU-4475 Mn3zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIX3TJBKSzVyPUWxNU4yODFizszN MnzKV2FPT0WU
P12-ICHIKAWA MkfhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4n3d2lEPTB;NUGxMlQyPiEQvF2= NX\4WIRrW0GQR1XS
NCI-H1694 NELUT5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTVzMj60N|kh|ryP NYnhfFVKW0GQR1XS
NCI-H2171 NVmyT|RIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoH3TWM2OD13MUKuO|A2KM7:TR?= MljkV2FPT0WU
Mo-T NUTUe49lT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTVzNT6xNVYh|ryP NVOwVWhoW0GQR1XS
HL-60 NH\mSWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXnJWlM1UUN3ME21NlIvQTJ2IN88US=> NXz4So9LW0GQR1XS
NCI-H1436 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nBSmlEPTB;NUKzMlA3PCEQvF2= NUX5PYVNW0GQR1XS
NCI-H510A NULXTJhGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2P4S2lEPTB;NUK0MlY6PiEQvF2= NFfmbm5USU6JRWK=
NCI-H2081 NIq2TFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmHmTWM2OD13MkWuOVE{KM7:TR?= M2TwRnNCVkeHUh?=
NB5 NV24WGcyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFm1c|NKSzVyPUWyOk41OyEQvF2= NH7lTnNUSU6JRWK=
NCI-H2227 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;pT21[UUN3ME21N|AvOjV2IN88US=> MVnTRW5ITVJ?
HSC-4 NFX5dFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml:1TWM2OD13M{muNlk{KM7:TR?= MkXTV2FPT0WU
RL MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2f3bGlEPTB;NUSyMlQ1OiEQvF2= M3naSXNCVkeHUh?=
EVSA-T MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml60TWM2OD13NEOuOlg1KM7:TR?= NW\jbHNFW0GQR1XS
NCI-H889 NH3t[VJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4q4fmlEPTB;NUS3MlI{KM7:TR?= MlrwV2FPT0WU
COLO-320-HSR NFnqW3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnL2TWM2OD13NUGuN|Uh|ryP NEHXNnlUSU6JRWK=
EW-11 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPrVnhKSzVyPUW1Nk4{QTlizszN MnTtV2FPT0WU
UACC-812 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDkTWM2OD13NUmuN|kyKM7:TR?= NIm2R2xUSU6JRWK=
CAL-148 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTV4NT6wPFkh|ryP MmjvV2FPT0WU
K052 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DTUWlEPTB;NUe5MlIyOyEQvF2= M3PBfnNCVkeHUh?=
L-428 NInodYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfJR|UxRTZyMD62O|Eh|ryP Mme1V2FPT0WU
LP-1 M3\EUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1fGZWlEPTB;NkCzMlU5PyEQvF2= NG\aWllUSU6JRWK=
ML-2 MnnoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlX6TWM2OD14ME[uOVc6KM7:TR?= M{XrZXNCVkeHUh?=
KARPAS-45 NV7reoZNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTVOlRKSzVyPU[xPE4zOjFizszN NUXSOWwyW0GQR1XS
HT NVrIWnA6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjEV|lFUUN3ME22NlIvPzh3IN88US=> NGPYdHZUSU6JRWK=
NCI-H226 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DmfGlEPTB;NkK4MlM2OyEQvF2= NVTNR3RRW0GQR1XS
L-363 Mm\US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HacmlEPTB;NkS4MlA3PCEQvF2= Ml7SV2FPT0WU
IM-9 NWjWWIZrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDn[5FtUUN3ME22OVEvPTRzIN88US=> MojCV2FPT0WU
TUR NV7Oc5NnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjORpBKSzVyPU[3NU4{OjJizszN M{HtV3NCVkeHUh?=
NCI-SNU-16 MlXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEizd4FKSzVyPU[4NE41PjJizszN MnjDV2FPT0WU
NCI-SNU-1 NXO5NVNYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVX0blVuUUN3ME22PVAvOTd{IN88US=> NVnheJZYW0GQR1XS
U-87-MG MkfxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHyTWM2OD14OUSuOVg2KM7:TR?= MXHTRW5ITVJ?
KM-H2 NIfOfJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1LQNWlEPTB;Nkm5MlA2QSEQvF2= MmHNV2FPT0WU
DEL Mlm2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3S1W2lEPTB;N{CwMlg{QCEQvF2= MoWyV2FPT0WU
KARPAS-422 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\VNpNKSzVyPUe4PU4{OTVizszN Ml34V2FPT0WU
BC-3 M1XwXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NY[1Woh6UUN3ME24NFUvOjB|IN88US=> MXrTRW5ITVJ?
NCI-H1155 NH36VIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2jPV2lEPTB;OEG4MlA2QSEQvF2= M3LYO3NCVkeHUh?=
NCI-H1581 NEjOcVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXXTWM2OD17MECuNlMyKM7:TR?= MXTTRW5ITVJ?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cleaved PARP / CD31 / VE-Cadherin / α-SMA / vimentin; 

PubMed: 30709379     


NCI-H460 cells were co-cultured with HUVECs in a 3D culture system, and then treated with 1 μM CHIR-99021, 10 μM or 20 μM Cisplatin, or a combination of both, for 24 h. Cells were harvested and immunoblotted with anti-cleaved PARP, anti-CD31, anti-VE-cadherin, anti-α-SMA, anti-vimentin, and anti-β-actin antibodies. 

CTNNB1; 

PubMed: 23993102     


Treatment with the GSK3β inhibitor CHIR-99021 led to increased levels of β-catenin in RKO and HT29 (non-mutant) cells.

β-catenin; 

PubMed: 24165128     


HeLa cells were treated with 5 μm CHIR-99021 for the indicated times. The samples were analyzed by Western blot.

30709379 23993102 24165128
Growth inhibition assay
Cell viability; 

PubMed: 22039301     


HMC-1.2 cells were cultured in the absence or presence of GSK3β inhibitor (CHIR 99021, 3–30 µM) and viability cell count was assessed by trypan blue dye exclusion after 24 h, 48 h or 72 h. All data are presented as the mean ± SEM of three independent experiments conducted in triplicate. p < 0.05*, p < 0.001** p < 0.0001*** vs vehicle treated control cells.

22039301
Immunofluorescence
SOX10 / EdU; 

PubMed: 28174705     


Incorporation of EdU (white arrowheads) into SOX10+ ENS (enteric nervous system) cells with and without CHIR-99021.

Oct3/4; 

PubMed: 24779365     


Fluorescence micrographs showing the protein expression of Brachyury/Oct3/4 after a three day differentiation of ES-D3 cells with BIO (0.5 μM), SB-216763 (5 μM), CHIR-99021 (5 μM), or CHIR-98014 (1 μM). Nuclei were counterstained with DAPI (blue). The merge is shown in yellow. Original magnification 400x. Scale bar = 50 μm.

28174705 24779365
In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. [1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. [4]

Protocol

Kinase Assay:

[1]

+ Expand

Cell-free kinase assays:

Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research:

[3]

+ Expand
  • Cell lines: INS-1E
  • Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM
  • Incubation Time: 1, or 4 days
  • Method:

    Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes
  • Formulation: Formulated as solutions in 20 mM citrate-buffered 15% Captisol or as fine suspensions in 0.5% carboxymethylcellulose.
  • Dosages: ~48 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (185.33 mM)
Ethanol 2 mg/mL (3.98 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
24mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 501.8
Formula

C22H18Cl2N8.HCl

CAS No. 1797989-42-4
Storage powder
in solvent
Synonyms N/A

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Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?

  • Answer:

    The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.

  • Question 2:

    How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?

  • Answer:

    Please change the culture medium each day to add the new comound in it if the duration is more than 72h.

  • Question 3:

    What is the difference between S2924 and S1263? Which one is better for differentiation?

  • Answer:

    S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID