CHIR-99021 (CT99021) HCl

Name: CHIR-99021 (CT99021) HCl
Brand: Selleck Chemicals
SKU: S2924
Rating: 5 (295 reviews)

For research use only.

Catalog No.S2924

295 publications

CHIR-99021 (CT99021) HCl Chemical Structure

CAS No. 1797989-42-4

CHIR-99021 (CT99021) HCl is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2. CHIR-99021 is a potent pharmacological activators of the Wnt/beta-catenin signaling pathway. CHIR-99021 significantly rescues light-induced autophagy and augments GR, RORα and autophagy-related proteins.

Selleck's CHIR-99021 (CT99021) HCl has been cited by 295 publications

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J Transl Med, 2020, 18(1):283

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EMBO Rep, 2020, e49863

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Metabolites, 2020, 10(8):E302

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Blood, 2019, blood.2019001037

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Proc Natl Acad Sci U S A, 2019, 116(39):19523-19529

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Cell Rep, 2019, 26(13):3643-3656

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Cell Rep, 2019,

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Elife, 2019, 8e41769

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BMC Biol, 2019, 17(1):89

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FASEB J, 2019, 33(2):2694-2706

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Stem Cells, 2019, 37(9):1158-1165

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Gastroenterology, 2018, 155(5):1508-1523.e10

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Mol Cell, 2018, 70(5):949-960

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Nat Commun, 2018, 9(1):5230

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Cell Rep, 2018, 25(7):1966-1981.e4

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Cell Rep, 2018, 24(2):489-502

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Cell Rep, 2018, 24(6):1610-1626

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Elife, 2018, 10.7554/eLife.40167

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Stem Cell Reports, 2018, 11(2):497-513

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Proteomics, 2018, 18(14):e1800102

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J Tissue Eng Regen Med, 2018, 12(2):349-359

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Cell Res, 2017, 27(6):815-829

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Br J Pharmacol, 2017, 174(17):2818-2831

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Cancer Lett, 2017, 403:195-205

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Biomater Sci, 2017, 5(8):1567-1578

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JCI Insight, 2017, 2(18)

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Elife, 2016, 5e21459

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Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description

Targets

GSK-3β ^[1] (Cell-free assay)	GSK-3α ^[1] (Cell-free assay)
6.7 nM	10 nM

In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. ^[1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). ^[2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. ^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
NCI-H1581	M{jWZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MnLXTWM2OD17MECuNlMyKM7:TR?=	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
NCI-H1155	NYLHTohIT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M2\2fmlEPTB;OEG4MlA2QSEQvF2=	MmDCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
BC-3	Ml3FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MWPJR\|UxRThyNT6yNFMh\|ryP	NEi2cpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
KARPAS-422	MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MXnJR\|UxRTd6OT6zNVUh\|ryP	MnnTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
DEL	NUXJfI5yT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MYLJR\|UxRTdyMD64N\|gh\|ryP	M2HhZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
KM-H2	MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NX3SSFFWUUN3ME22PVkvODV7IN88US=>	M4jLfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
U-87-MG	NXTme\|dlT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NF7VSohKSzVyPU[5OE42QDVizszN	NXzwcFRiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
NCI-SNU-1	MmS4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MXXJR\|UxRTZ7MD6xO\|Ih\|ryP	Ml\4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
NCI-SNU-16	NV7pSoRbT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MXPJR\|UxRTZ6MD60OlIh\|ryP	M4TBXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
TUR	NF7jXohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MoPQTWM2OD14N{GuN\|IzKM7:TR?=	MnH1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
IM-9	MkP6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NUHCT3JsUUN3ME22OVEvPTRzIN88US=>	MnrhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
L-363	M1nrW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M2DneGlEPTB;NkS4MlA3PCEQvF2=	M4LUW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
NCI-H226	NXjGe\|RZT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M4jjfWlEPTB;NkK4MlM2OyEQvF2=	M364cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
HT	MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M3zHTmlEPTB;NkKyMlc5PSEQvF2=	NILNT409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
KARPAS-45	MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NFvVTWJKSzVyPU[xPE4zOjFizszN	NYTFUZlMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
ML-2	NIPKb49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MXTJR\|UxRTZyNj61O\|kh\|ryP	NWe5NllrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
LP-1	NX30XGhpT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MXnJR\|UxRTZyMz61PFch\|ryP	NX\RSY9[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
L-428	MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				Mny0TWM2OD14MECuOlcyKM7:TR?=	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
K052	MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NGriRWdKSzVyPUW3PU4zOTNizszN	MmfTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
CAL-148	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M4fyR2lEPTB;NU[1MlA5QSEQvF2=	NFHBSog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
UACC-812	NWXsXIpDT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MXvJR\|UxRTV3OT6zPVEh\|ryP	NYD0U5lERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
EW-11	NFL4NZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MkTzTWM2OD13NUKuN\|k6KM7:TR?=	MnP1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
COLO-320-HSR	NX:2ZnFKT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M3jRdGlEPTB;NUWxMlM2KM7:TR?=	NYrhcXBmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
NCI-H889	M4TIOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M33LOmlEPTB;NUS3MlI{KM7:TR?=	MmqzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
EVSA-T	NEXzOIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MYHJR\|UxRTV2Mz62PFQh\|ryP	M3rzdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
RL	NXXFcVNQT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M{jycWlEPTB;NUSyMlQ1OiEQvF2=	M{XQXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
HSC-4	M3S1Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MXvJR\|UxRTV\|OT6yPVMh\|ryP	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
NCI-H2227	MmnaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NVe2b2pXUUN3ME21N\|AvOjV2IN88US=>	NFPuUXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
NB5	MmntS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NGDodlVKSzVyPUWyOk41OyEQvF2=	M4rsRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
NCI-H2081	NVztfok5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MoXqTWM2OD13MkWuOVE{KM7:TR?=	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
NCI-H510A	MofMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MY\JR\|UxRTV{ND62PVYh\|ryP	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
NCI-H1436	NX3FdWRZT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				Mn7vTWM2OD13MkOuNFY1KM7:TR?=	Mlv0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
HL-60	NHXPNIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MkXvTWM2OD13MkKuPVI1KM7:TR?=	NEX3W4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
Mo-T	MnLrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NUXmbFF[UUN3ME21NVUvOTF4IN88US=>	Ml;DQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
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SF539	MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M33MfGlEPTB;MUSuNFEyPiEQvF2=	Ml7GQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
SW48	M2nlbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MULJR\|UxRTF\|LkeyOlUh\|ryP	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
MEL-HO	NY\pPWpxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				Mlj2TWM2OD1zMz64PFI6KM7:TR?=	NVXqN4c{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
KU812	MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NYXObmZTUUN3ME2xN{42OTd3IN88US=>	NYLQd\|dTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
T98G	NVTwUXo{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MVvJR\|UxRTF\|LkS3NVch\|ryP	MlSxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
DSH1	NUjJN5Q6T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NHPSUWtKSzVyPUGzMlM5PzlizszN	M{GzXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
NCI-H82	MoLXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M2\5cGlEPTB;MUOuNlkxQSEQvF2=	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
C8166	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MV;JR\|UxRTF\|LkKzNVgh\|ryP	NX7BT3lURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
PC-14	MlfqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NFzMdZVKSzVyPUGyMlMzPCEQvF2=	NUjYUmt[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
PA-1	MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MnTGTWM2OD1zMT65N{DPxE1?	NYrzTnBnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
HEL	NHjuXVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MknxTWM2OD1zMT62OVc6KM7:TR?=	NEW1Vm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
NCI-H526	M{PWUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NH;kO3JKSzVyPUGxMlE{PjVizszN	M1vveFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SNU-387	Mo[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MULJR\|UxRTFyLkeyN\|Uh\|ryP	NGrSSY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
HT-3	MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NHu3emlKSzVyPUGwMlYyPzlizszN	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
M059J	M{f6[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NE[4botKSzVyPUGwMlYyODJizszN	M3H3cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
MZ2-MEL	NULINWx6T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				Ml3nTWM2OD1zMD60Olc4KM7:TR?=	MorZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
NCI-H28	MoHKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NXKxdVR4UUN3ME2xNE41PDF\|IN88US=>	M{eyfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
LB1047-RCC	NF7kUWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NI\s[JdKSzVyPUmuPVk4QThizszN	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
U-118-MG	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M3jRbGlEPTB;OT65OFQ1PCEQvF2=	NGX4UGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
DBTRG-05MG	NVi2[3BYT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NIfuNYRKSzVyPUmuO\|c4PyEQvF2=	M334UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
EFM-19	M1HxdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NYi4VmVUUUN3ME25Mlc2PTZ4IN88US=>	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
SK-N-DZ	M4q1OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MV3JR\|UxRTlwNkW3OFIh\|ryP	M3P5T\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
LB996-RCC	M3nHVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MkL4TWM2OD17LkWyN\|k4KM7:TR?=	Moq3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
U-2-OS	Ml24S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				Mn;CTWM2OD17LkS1NFA3KM7:TR?=	MkPYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
CTV-1	NYLKdm5VT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NXzNbHZlUUN3ME25MlQ{QDl2IN88US=>	NYGzOHBTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
COLO-205	MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NWSySZFJUUN3ME25MlQ{QDJzIN88US=>	Mn;ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
NCI-H64	NFGxTYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				Mn[2TWM2OD17LkSyNlU2KM7:TR?=	NVrzSJRNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
NCI-H1755	MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MnvHTWM2OD17LkO5PVg3KM7:TR?=	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
ACHN	M2W1Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NXPEUopFUUN3ME25MlMxPTR4IN88US=>	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
MEG-01	NVi5TW5iT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MWDJR\|UxRThwOUmwOFMh\|ryP	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
MKN28	NIHXR3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M2\2V2lEPTB;OD64PFUyPiEQvF2=	MoHPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
CAL-72	Ml\6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NE\UeplKSzVyPUiuO\|YyOTNizszN	Ml:wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MV-4-11	MkL0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				Mn:2TWM2OD16LkW0N\|A1KM7:TR?=	M4rnTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SW1417	Mlv6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				Mlu5TWM2OD15Lk[0OVg1KM7:TR?=	M4\5XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
G-361	MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MnfyTWM2OD15LkS2OVYyKM7:TR?=	MnK2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MC116	NF2xN2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MYrJR\|UxRTdwMki2PVEh\|ryP	M2r0VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
RT-112	NYHFOnVwT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MnLFTWM2OD15LkG2OFU3KM7:TR?=	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
CAL-85-1	MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				Mm\OTWM2OD14Lk[wPVM3KM7:TR?=	NXzH[WNQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
BB65-RCC	M{TESWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MlK5TWM2OD14LkWzNVk2KM7:TR?=	NXnYWnB7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
CGTH-W-1	NWPHUVZsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M1L4c2lEPTB;Nj60NVch\|ryP	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
WM-115	MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NHu5W5BKSzVyPUWuPFE2ODlizszN	NGPvdXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
SK-MEL-2	M{DuNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M1LndmlEPTB;Nj6zPFM1PCEQvF2=	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
BL-41	NVO3[GhtT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MX\JR\|UxRTVwOEGyOlYh\|ryP	MkLNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
LB2518-MEL	MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NXjqc2ZMUUN3ME21MlczPjJ5IN88US=>	M4jxcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
D-392MG	NUHPZXlXT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MofzTWM2OD13LkS5NVQ2KM7:TR?=	MonlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
NCI-H2452	MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MUfJR\|UxRTVwNEW2Nlgh\|ryP	M4OzdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SW1710	M1z4cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M1;xd2lEPTB;NT60NlQ2PiEQvF2=	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
SK-OV-3	NIW4dVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M{DHPGlEPTB;ND65PVM1KM7:TR?=	M2LROFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
ALL-PO	MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MkLhTWM2OD12LkizPFU5KM7:TR?=	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
BxPC-3	Mmn5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				Mn\TTWM2OD12LkewN\|kyKM7:TR?=	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
RCC10RGB	M13jeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MVrJR\|UxRTRwMkm0O\|kh\|ryP	NUTSV2hwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
NKM-1	MkTmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MWDJR\|UxRTRwMUO5O\|Eh\|ryP	NGTLZ5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
MHH-ES-1	M{fkRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NV32O49sUUN3ME20MlA1PTR3IN88US=>	NFvNfVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
OE19	MkmyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				Mn\1TWM2OD1\|Lkm2OFUyKM7:TR?=	M{XUOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
TE-15	MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M3;sOGlEPTB;Mz65OVM1OyEQvF2=	NYLvWVdDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
MOLT-16	NGrXbI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1;hbWlEPTB;Mz64PFU2PiEQvF2=	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
A172	NX;EcYNrT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MkPzTWM2OD1\|Lke1OFk5KM7:TR?=	M3jlR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
HCC1569	NEP2cIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MXXJR\|UxRTNwNEizNFUh\|ryP	MlL5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
HOP-62	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NIn6bIVKSzVyPUOuN\|YxPTRizszN	MnSzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
A704	Mmf1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MlPOTWM2OD1\|LkC4NVIh\|ryP	M{S2fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SCC-9	NEnXZ\|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NG\CW5VKSzVyPUKuPVU1QDVizszN	NHr2UWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
COLO-829	MmHLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M4m0WGlEPTB;Mj63OlU1KM7:TR?=	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
RPMI-7951	NHHOcmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M13FemlEPTB;Mj63NFE6OyEQvF2=	Ml3vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
SK-LMS-1	Ml\5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MXHJR\|UxRTJwNkSxNlgh\|ryP	M3u3OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SNU-423	M4\hOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NVy5Nm5NUUN3ME2yMlQ6Pzh5IN88US=>	Mn;UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
NCI-H2122	M3rje2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				Mn;2TWM2OD1{LkK3Olg1KM7:TR?=	NGj2W5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
RS4-11	MmfES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MnLMTWM2OD1zLkm1OFE2KM7:TR?=	M3;CUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
HCC1395	M4\IZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NVexUodxUUN3ME2xMlc5Ojd\|IN88US=>	Mn;SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MIA-PaCa-2	NEDtcZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MmnxTWM2OD1zLke1PVc4KM7:TR?=	NGrQO\|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
KYSE-180	NYHP[m5sT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M2nRXWlEPTB;MT63Nlc5QCEQvF2=	MoXJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
BCPAP	NF7ne5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MmPJTWM2OD1zLk[2PFk5KM7:TR?=	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
A498	M2rER2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NXTxXnpZUUN3ME2xMlMxPDZ7IN88US=>	MlTkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
TYK-nu	M3PZ[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NEXNW5ZKSzVyPUGuNFc6OTJizszN	MlvIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
D-247MG	Mo[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NWHWXnJRUUN3ME2wMlk1QDV{IN88US=>	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
S-117	MoC3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MlnRTWM2OD1yLkmyNVMyKM7:TR?=	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
D-336MG	NHHsPW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NV;5d4dxUUN3ME2wMlg1PjJizszN	MkTVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
LOXIMVI	MkX3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M1PqXGlEPTB;MD61PFg{OiEQvF2=	M4T4WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
769-P	NYDwN\|hpT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M1W3PWlEPTB;MD61PFQ6QSEQvF2=	NVHkflgzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
GCT	MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NUDXc3ppUUN3ME2wMlMxPjd{IN88US=>	M1vBSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SW982	NHXENVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NUGy[I9xUUN3ME2wMlIyPDF4IN88US=>	M1;udFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
ES-CCE	NVe2VVhmTnWwY4Tpc44hSXO\|YYm=	NI\QbYk2yqEQvF2=	NGDyUYU{KGR?	MUPEUXNQ	MWLTeJJwdmeueTDpcoR2[2W\|IITo[UBi[3Srdnn0fUBw\iCZboSvZoV1[S2lYYTlcolvKHCjdHj3ZZk>	NVjLe4N1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3O\|k{PjVpPkK0O\|c6OzZ3PD;hQi=>
ES-D3	MUnGeY5kfGmxbjDBd5NigQ>?	NGXHWmQ2yqEQvF2=	MVezJIQ>	NIDyRVBFVVOR	MkHvV5Rzd26pbImgbY5lfWOnczD0bIUh[WO2aY\peJkhd2ZiV370M4JmfGFvY3H0[Y5qdiCyYYToe4F6	MlTlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5N{mzOlUoRjJ2N{e5N\|Y2RC:jPh?=
ES-CCE	NET3O5VEgXSxdH;4bYMhSXO\|YYm=	NWHlOWZ5OTEEoN88US=>	MlO0N{Bl	MV;EUXNQ	MlfqTY5lfWOnczDtc4RmemG2ZTDjfZRwfG:6aXPpeJk>	M2SzO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{e5N\|Y2Lz5{NEe3PVM3PTxxYU6=
ES-D3	Mnm3R5l1d3SxeHnjJGF{e2G7	M3jzRlExyqEQvF2=	M333bFMh\A>?	NYXrblBRTE2VTx?=	M{nnN2lv\HWlZYOgcY9l\XKjdHWgZ5l1d3SxeHnjbZR6KHerdHigTWM2OCCxZjC0Mlkh\|ryP	NXexPYk5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3O\|k{PjVpPkK0O\|c6OzZ3PD;hQi=>
Neural progenitor cells	NIHqbYNHfW6ldHnvckBCe3OjeR?=	MXuzJO69VQ>?	M2LyTlExKGR?	NVPUV2N{TE2VTx?=	MUPJcoR2[2W\|IHflcoVz[XSrb36gc4YhdmW3cnHsJJBzd2enbnn0c5Ih[2WubIO=	NFjVWYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[zPFA{PCd-MkS2N\|gxOzR:L3G+
A549	MlXYRZBweHSxc3nzJGF{e2G7	NFjm[XY2KML3TR?=	MYqyOEBp	MWHEUXNQ	M4CxWGlv[3KnYYPld{Bk\WyuIHHwc5B1d3Orcx?=	M4fKUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{[3Olg5Lz5{NEO2O\|Y5QDxxYU6=
H157	NX\KfYtzSXCxcITvd4l{KEG\|c3H5	NGKwZmc2KML3TR?=	MVqyOEBp	MmfpSG1UVw>?	M2Lwd2Rw\XNibn;0JJNq\26rZnnjZY51dHliYX\m[YN1KGGyb4D0c5Nqew>?	NEDYTIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2O\|Y5QCd-MkSzOlc3QDh:L3G+
H460	M{nWPWFxd3C2b4Ppd{BCe3OjeR?=	NWfiXW92PSEEtV2=	MkG3NlQhcA>?	NFnufpBFVVOR	M1G3RWF1fGWwdXH0[ZMh[2WubDDhdI9xfG:\|aYO=	M2O4blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{[3Olg5Lz5{NEO2O\|Y5QDxxYU6=
H157	M1HWUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NIHkOmY2KML3TR?=	MnfWNlQhcA>?	NHfnR29FVVOR	MUPQdo9ud3SnczDj[YxtKHC{b3zp[oVz[XSrb36=	NEnpdVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2O\|Y5QCd-MkSzOlc3QDh:L3G+
H460	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NH72fVU2KML3TR?=	NFi2TXIzPCCq	NULH[2djTE2VTx?=	MUfQdo9ud3SnczDj[YxtKHC{b3zp[oVz[XSrb36=	NEHl[II9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2O\|Y5QCd-MkSzOlc3QDh:L3G+
embryonic stem cells (ESC)	M3zq[2Z2dmO2aX;uJGF{e2G7	MWGzJOK2VQ>?	NUfNdYN{OTFiZB?=	MYrEUXNQ	NH7tdXdRem:vb4Tld{B{\WyoLYLlcoV4[Wxib3[gcoHEt3[nLYT5dIUhdW:3c3Wg[Y1jenmxbnnjJJN1\W1iY3XscJMhMEWVQzm=	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh2M{OxNkc,OjN6NEOzNVI9N2F-
DN3	M1vB[WZ2dmO2aX;uJGF{e2G7	NWnEfGloOTBizszN	NFqyN3Q6PiCq	Mmm5SG1UVw>?	M1;Ce2lv[3KnYYPld{Bk\WyuIHTleoVtd3CvZX70JIFv\CCycn;sbYZmemG2aX;u	NEfSfGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WyOlk5QSd-MkO1NlY6QDl:L3G+
platelets	M4nhemZ2dmO2aX;uJGF{e2G7	NVi2UYhFOTBizszN	M3q0[VE2KG2rbh?=	MYDEUXNQ	M4\pPGlv[3KnYYPld{B1cHKxbXLpck1u\WSrYYTl[EBxdGG2ZXzleEBi\2e{ZXfheIlwdiCjbnSgRXRRKHOnY4LleIlwdg>?	M1zLU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MkO5PFc4Lz5{M{KzPVg4PzxxYU6=
hiPSC-NPs	MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NEDiWZg{6oDLzszN	M1X5OFczKGh?	Mkn2SG1UVw>?	M3HxPWlv[3KnYYPld{Bk\WyuIIDyc4xq\mW{YYTpc47DqA>?	Mlz3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEK2PFcoRjJ{NkSyOlg4RC:jPh?=
INS-1E	MmLESpVv[3Srb36gRZN{[Xl?	M3nuTVIvPSEQvF2=	NGnx[XczPCCq	NHXmW49FVVOR	NYHmPJpyUW6mdXPld{BiKHKnZIXjeIlwdiCxZjDOVFEhd3[ncnX4dJJme3Orb36=	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{N{ewOEc,OjJ2Mke3NFQ9N2F-
PANC1	NEixfo1CeG:ydH;zbZMhSXO\|YYm=	MUhCpFUh\|ryP	MmDkO\|IhcA>?	NH\leXBFVVOR	NXiy[GVuXHKrZ3fldpMh[W5iYYDvdJRwfGmlIILld5BwdnOn	MlHtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{MEGxPFYoRjJ{MkCxNVg3RC:jPh?=
Endothelial cell(EC)	MYTGeY5kfGmxbjDBd5NigQ>?	NVXLPW1MOyEQvF2=		M4jRcWROW09?	MnzoVJJwdW:2ZYOg[Y5ld3SqZXzpZYwh[2WubDCoSWMqKGSrZn\ldoVvfGmjdHnvci=>	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB3MkCwOkc,OjJyNUKwNFY9N2F-
HMC1.2	NGrtVVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MljRN\|Ah\|ryP	NYfme3ZOPzJiaB?=	M2LZSGROW09?	MWfS[YR2[2W\|IHPlcIwhe3W{dnn2ZYw>	NXT4fJoxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwN\|k{ODFpPkKyNFM6OzBzPD;hQi=>
Sca-1+ HPC	M{X5S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NVHJOoxtOiEQvF2=	M4\tclYh\A>?	MX\EUXNQ	MoqzV5lv\XKpaYP0bYNidGy7IIDyc41wfGW\|IGPjZU0yM8LiSGDDJJBzd2yrZnXyZZRqd25iYX7kJINwdG:weTDmc5Ju[XSrb36geI9o\XSqZYKge4l1cCCHR1[=	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTZ{NEO0PEc,OjF4MkSzOFg9N2F-
Oct4/Sox2/Klf4-infected MEFs	MYLGeY5kfGmxbjDBd5NigQ>?	M3:yR\|ExKM7:TR?=	MmjrNlgh\A>?	NXTRRmQ6TE2VTx?=	MmDvVJJwdW:2ZYOgUWVHeyC{ZYDyc4dz[W2vaX7nJHRz[W6\|ZIXj[YQh[nliT3P0OEwhW2:6MjygZY5lKEuuZkS=	NHjvUos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUizPVA2PSd-MUm4N\|kxPTV:L3G+
H4IIE	NVvrTYJTTnWwY4Tpc44hSXO\|YYm=	MmP1NVAh\|ryP	MlXuNlQhcA>?	M1fHUmROW09?	M2DMfHJm\3WuYYTld{DPui2lYYTlcolvKGGldHn2bZR6KGGwZDDUTXJGKGOxboThbY5qdmdicILvcY91\XJiYXP0bZZqfHl?	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTN3MEG5OUc,OTV\|NUCxPVU9N2F-
insulin receptor–expressing CHO-IR	MXrGeY5kfGmxbjDBd5NigQ>?	Mk\lOVAxKG6P	MWqzNEBucW5?	MmfsSG1UVw>?	NHP4bXVC[3SrdnH0[ZMhT2y7Y3;n[Y4hW3mwdHjhd4UhMEeVKR?=	NIHwcWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMk[wOlQ6Pyd-MUK2NFY1QTd:L3G+
3T3-L1	Mn[2SpVv[3Srb36gRZN{[Xl?	MWOzJO69VQ>?	MVG0JIg>	MX7EUXNQ	M2K3[mlvcGmkaYTzJJBz\WGmaYDvZ5l1\SCmaX\m[ZJmdnSrYYTpc44>	MkDoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJyNUWyNFAoRjF{MEW1NlAxRC:jPh?=
CHO-IR	NGLscI9HfW6ldHnvckBCe3OjeR?=		NXT2dVV1OzBibXnu		M1jGNmVEPTB;MD63O{DPxE1?	M1PhUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MEG2NVIyLz5{OUCxOlEzOTxxYU6=
HepG2	M{PGUmZ2dmO2aX;uJGF{e2G7		NWfze3d3OyCq		MmLpSWM2OD1zLkWg{txO	NW\aSZk5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlExOjNpPkKyNlYyODJ\|PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Cleaved PARP / CD31 / VE-Cadherin / α-SMA / vimentin; PubMed: 30709379 J Exp Clin Cancer Res NCI-H460 cells were co-cultured with HUVECs in a 3D culture system, and then treated with 1 μM CHIR-99021, 10 μM or 20 μM Cisplatin, or a combination of both, for 24 h. Cells were harvested and immunoblotted with anti-cleaved PARP, anti-CD31, anti-VE-cadherin, anti-α-SMA, anti-vimentin, and anti-β-actin antibodies. CTNNB1; PubMed: 23993102 Cell Treatment with the GSK3β inhibitor CHIR-99021 led to increased levels of β-catenin in RKO and HT29 (non-mutant) cells. β-catenin; PubMed: 24165128 J Biol Chem HeLa cells were treated with 5 μm CHIR-99021 for the indicated times. The samples were analyzed by Western blot.	30709379 23993102 24165128
Growth inhibition assay	Cell viability; PubMed: 22039301 J Immunol HMC-1.2 cells were cultured in the absence or presence of GSK3β inhibitor (CHIR 99021, 3–30 µM) and viability cell count was assessed by trypan blue dye exclusion after 24 h, 48 h or 72 h. All data are presented as the mean ± SEM of three independent experiments conducted in triplicate. p < 0.05, p < 0.001* p < 0.0001*** vs vehicle treated control cells.	22039301
Immunofluorescence	SOX10 / EdU; PubMed: 28174705 Cell Mol Gastroenterol Hepatol Incorporation of EdU (white arrowheads) into SOX10+ ENS (enteric nervous system) cells with and without CHIR-99021. Oct3/4; PubMed: 24779365 BMC Res Notes Fluorescence micrographs showing the protein expression of Brachyury/Oct3/4 after a three day differentiation of ES-D3 cells with BIO (0.5 μM), SB-216763 (5 μM), CHIR-99021 (5 μM), or CHIR-98014 (1 μM). Nuclei were counterstained with DAPI (blue). The merge is shown in yellow. Original magnification 400x. Scale bar = 50 μm.	28174705 24779365

In vivo

Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. ^[1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. ^[4]

Protocol

Kinase Assay: ^[1]	- Collapse Cell-free kinase assays: Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl₂, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research: ^[3]	- Collapse Cell lines: INS-1E Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM Incubation Time: 1, or 4 days Method: Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system. (Only for Reference)
Animal Research: ^[1]	- Collapse Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes Dosages: ~48 mg/kg Administration: Orally (Only for Reference)

References

Solubility (25°C)

In vitro	DMSO	93 mg/mL (185.33 mM)
	Ethanol	2 mg/mL (3.98 mM)
	Water	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 5% DMSO+30% PEG 300+ddH2O For best results, use promptly after mixing.	24mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download CHIR-99021 (CT99021) HCl SDF

Molecular Weight	501.8
Formula	C₂₂H₁₈Cl₂N_8.HCl
CAS No.	1797989-42-4
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	CC1=CN=C(N1)C2=CN=C(N=C2C3=C(C=C(C=C3)Cl)Cl)NCCNC4=NC=C(C=C4)C#N.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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The Serial Dilution Calculator Equation

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Computed Result

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Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?
Answer:
The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.
Question 2:
How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?
Answer:
Please change the culture medium each day to add the new comound in it if the duration is more than 72h.
Question 3:
What is the difference between S2924 and S1263? Which one is better for differentiation?
Answer:
S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

GSK-3 Signaling Pathway Map

GSK-3 Inhibitors with Unique Features

Selective GSK-3 Inhibitor

TWS119 : GSK-3β-selective, IC50 of 30 nM.
Most Potent GSK-3 Inhibitor

CHIR-98014 : GSK-3α, IC50=0.65 nM; GSK-3β, IC50=0.58 nM.
GSK-3 Inhibitor in Clinical Trial

Tideglusib : Phase II for Alzheimer's disease.
Newest GSK-3 Inhibitor

AZD1080 : Orally active, brain permeable GSK3 inhibitor, inhibits human GSK3α and GSK3β with K_i of 6.9 nM and 31 nM, respectively.

Related GSK-3 Products

CHIR-99021 (CT99021) ^New

CHIR-99021 (CT99021) is a GSK-3α and GSK-3β inhibitor with IC50 of 10 nM and 6.7 nM, respectively. CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3β compared to CDKs. CHIR99021 functions as a Wnt/β-catenin activator and induces autophagy.
LY2090314 ^New

LY2090314 is a potent GSK-3 inhibitor for GSK-3α/β with IC50 of 1.5 nM/0.9 nM; may improve the efficacy of platinum-based chemotherapy regimens. LY2090314 is highly selective towards GSK3 as demonstrated by its fold selectivity relative to a large panel of kinases.
TDZD-8 ^New

TDZD-8 (NP 01139) is a non-ATP competitive GSK-3β inhibitor with IC50 of 2 μM; minimal inhibitory effect observed on CDK1, casein kinase II, PKA and PKC.
TWS119

TWS119 is a GSK-3β inhibitor with IC50 of 30 nM in a cell-free assay; capable of inducing neuronal differentiation and may be useful to stem cell biology. GSK-3β inhibition triggers autophagy.
SB216763

SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β. SB216763 activates autophagy.
Tideglusib

Tideglusib (NP031112, NP-12) is an irreversible, non ATP-competitive GSK-3β inhibitor with IC50 of 60 nM in a cell-free assay; fails to inhibit kinases with a Cys homologous to Cys-199 located in the active site. Phase 2.
CHIR-98014

CHIR-98014 (CT98014) is a potent GSK-3α/β inhibitor with IC50 of 0.65 nM/0.58 nM in cell-free assays, with the ability to distinguish GSK-3 from its closest homologs Cdc2 and ERK2.
BIO

BIO (GSK-3 Inhibitor IX, 6-bromoindirubin-3-oxime, 6-Bromoindirubin-3'-oxime, MLS 2052) is a specific inhibitor of GSK-3 with IC50 of 5 nM for GSK-3α/β in a cell-free assay, shows >16-fold selectivity over CDK5, also a pan-JAK inhibitor with IC50 of 30 nM for Tyk2. BIO induces apoptosis in human melanoma cells.

Features:The first pharmacological agent shown to maintain self-renewal in human and mouse embryonic stem cells.
AR-A014418

AR-A014418 (GSK-3β Inhibitor VIII) is an ATP-competitive, and selective GSK3β inhibitor with IC50 and K_i of 104 nM and 38 nM in cell-free assays, without significant inhibition on 26 other kinases tested.
IM-12

IM-12 is a selective GSK-3β inhibitor with IC50 of 53 nM, and also enhances canonical Wnt signalling.

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CHIR-99021 (CT99021) HCl