CHIR-99021 (CT99021) HCl

Name: CHIR-99021 (CT99021) HCl
Brand: Selleck Chemicals
SKU: S2924
Rating: 5 (374 reviews)

For research use only.

Catalog No.S2924

374 publications

CHIR-99021 (CT99021) HCl Chemical Structure

CAS No. 1797989-42-4

CHIR-99021 (CT99021) HCl is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2. CHIR-99021 is a potent pharmacological activators of the Wnt/beta-catenin signaling pathway. CHIR-99021 significantly rescues light-induced autophagy and augments GR, RORα and autophagy-related proteins.

Selleck's CHIR-99021 (CT99021) HCl has been cited by 374 publications

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EMBO Rep, 2020, e49863

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Bachelor of Science, 2012, East China Normal University

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Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description

Targets

GSK-3β ^[1] (Cell-free assay)	GSK-3α ^[1] (Cell-free assay)
6.7 nM	10 nM

In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. ^[1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). ^[2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. ^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
NCI-H1581	M4P6NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MUfJR\|UxRTlyMD6yN\|Eh\|ryP	M1LXPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
NCI-H1155	M3PKcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MlLDTWM2OD16MUiuNFU6KM7:TR?=	NX7HV3pNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
BC-3	MnjCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M1LTR2lEPTB;OEC1MlIxOyEQvF2=	NVLr[It[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
KARPAS-422	M1X3WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NVrnOphXUUN3ME23PFkvOzF3IN88US=>	M1HL[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
DEL	NXvKeWJKT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MWPJR\|UxRTdyMD64N\|gh\|ryP	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
KM-H2	NGfXRoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NEnPdGNKSzVyPU[5PU4xPTlizszN	NXvaRWJyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
U-87-MG	MkjwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MlXtTWM2OD14OUSuOVg2KM7:TR?=	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
NCI-SNU-1	NVjjSoI1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NEfVNmlKSzVyPU[5NE4yPzJizszN	NGDiNHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
NCI-SNU-16	MoP1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NF\MSoRKSzVyPU[4NE41PjJizszN	Mm\hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
TUR	NVK3VVJGT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MXPJR\|UxRTZ5MT6zNlIh\|ryP	NHPkOVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
IM-9	NV;CRoF{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MX7JR\|UxRTZ3MT61OFEh\|ryP	NXrye3VVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
L-363	NHXUUXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MXjJR\|UxRTZ2OD6wOlQh\|ryP	NH7r[FM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
NCI-H226	M1zS[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NVvmUnB4UUN3ME22NlgvOzV\|IN88US=>	M4\jXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
HT	Mk\MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MVLJR\|UxRTZ{Mj63PFUh\|ryP	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
KARPAS-45	MlnrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NELNTpNKSzVyPU[xPE4zOjFizszN	NUf1VlQ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
ML-2	MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MV7JR\|UxRTZyNj61O\|kh\|ryP	Mln6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
LP-1	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MmH0TWM2OD14MEOuOVg4KM7:TR?=	M{fjWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
L-428	MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				Mlv2TWM2OD14MECuOlcyKM7:TR?=	NEHIOmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
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ETK-1	Ml\DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MUXJR\|UxRTF7MD6xPFEh\|ryP	MmDGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
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CAPAN-1	NYeyc5duT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NGPtdZNKSzVyPUG4O{41PTNizszN	Moj3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
MONO-MAC-6	NGLQdm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M{XBc2lEPTB;MUi3MlM6OiEQvF2=	M{HMPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
MDA-MB-453	NW\ucFZyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M1zVbmlEPTB;MUi2Mlk1OSEQvF2=	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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LB2241-RCC	NIXKXXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M{TJXGlEPTB;MUi1MlE1PSEQvF2=	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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NCI-H522	M1WzSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MnHoTWM2OD1zOEGuNFU6KM7:TR?=	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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COLO-741	MmG5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MX;JR\|UxRTF5Nz61OlEh\|ryP	NFTmXZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
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LNCaP-Clone-FGC	MlvXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NHXhUGRKSzVyPUG3OU45PjFizszN	M{H3XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
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HTC-C3	M4qzOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MWjJR\|UxRTF2NT6wO\|Uh\|ryP	MoC4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
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NCI-H810	NXXLWGFvT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NXrXSIk5UUN3ME2xOFMvPjh5IN88US=>	NHO5NFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
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YKG-1	MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NXvrc5pGUUN3ME2xOFIvQDh7IN88US=>	NEPrOHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
OE33	NX3RVXg1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NILBdoVKSzVyPUG0NU44QThizszN	MmS1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
OCI-AML2	NX\BZm1kT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MmrrTWM2OD1zNEGuOVA4KM7:TR?=	NIfHV4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
Detroit562	NXPuVVBTT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NV\oWIxQUUN3ME2xOFEvPDZ4IN88US=>	NE\RfGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
OVCAR-4	M3jWWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NVL4UXJ2UUN3ME2xOFAvPzR7IN88US=>	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
BHT-101	M2LUfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MYXJR\|UxRTF2MD6yNFgh\|ryP	M{Xid\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
Ca-Ski	MmXxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MoDRTWM2OD1zM{iuPFMyKM7:TR?=	M2rvbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
OAW-42	MnLCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M33wd2lEPTB;MUO3Mlg2PiEQvF2=	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
G-402	M4rmbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M3TQ[WlEPTB;MUO1MlI6PSEQvF2=	M4Ll[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
NOS-1	MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MWXJR\|UxRTF\|ND63OlUh\|ryP	M3fuVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
NCI-H1437	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MoXSTWM2OD1zM{SuOVkh\|ryP	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
JEG-3	NH3MZoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MULJR\|UxRTF\|ND6zOVkh\|ryP	NVL6SGlPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
CP50-MEL-B	MmjyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M4nLR2lEPTB;MUO0MlIh\|ryP	NFvLZVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
BB30-HNC	NWi0XZdLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NVrMTpdJUUN3ME2xN\|QvOTF4IN88US=>	NHjmNW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
Capan-2	MkGxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NXf5XG15UUN3ME2xN\|MvQTd3IN88US=>	NXLoNnZvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
CTB-1	MoTjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NHruR3NKSzVyPUGzNk45QTdizszN	NG\uR209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
HT-1080	MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MYHJR\|UxRTF\|Mj6yNFQh\|ryP	NEnETnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
MFH-ino	M4D5Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M3XxSWlEPTB;MUOxMlEyPCEQvF2=	NEnEbnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
VMRC-RCZ	NUXJXod3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MUTJR\|UxRTF\|MD6zO\|Uh\|ryP	M4LHcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
LU-99A	MmfSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NWLJU2hGUUN3ME2xNlkvQTNzIN88US=>	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
HEC-1	M1\weWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M1;3VWlEPTB;MUK5MlE6QCEQvF2=	NUPteZY4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
EB-3	MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MoLaTWM2OD1zMkeuNFE{KM7:TR?=	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
UMC-11	NIKwc5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NWflb3h2UUN3ME2xNlYvODN6IN88US=>	Mm[1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
NCI-H2087	NULJSJlsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NEXhUpFKSzVyPUGyOE4zQDNizszN	NYTDd4ZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
SW1463	NX;BOVlkT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NWLXcpJVUUN3ME2xNlQvODd5IN88US=>	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
OPM-2	NEnYe5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NW\qUWpvUUN3ME2xNlMvOTd4IN88US=>	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
HT-29	MnXVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NE\KXHRKSzVyPUGyNk44PzhizszN	MkKxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
HAL-01	M4XHVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MWDJR\|UxRTF{Mj60PVkh\|ryP	NIr5dGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
KYSE-140	MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MoHWTWM2OD1zMkKuNVQyKM7:TR?=	M{PjPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
HuP-T4	MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M2PCW2lEPTB;MUKxMlU2KM7:TR?=	NGPhO4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
NCI-N87	NF:xW4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NFXKOGhKSzVyPUGyNU4xPDRizszN	NYDjW\|lHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
OVCAR-3	NGrNb5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MYHJR\|UxRTFzOT65NVUh\|ryP	NIfDW2k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
KNS-81-FD	Mn3vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M3PQOmlEPTB;MUG5MlQyPyEQvF2=	M2[5T\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SUP-T1	M1HxTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NEe3NmpKSzVyPUGxPE46PTZizszN	M2TScVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
SKG-IIIa	MlLqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NX7iV5RMUUN3ME2xNVgvOTF4IN88US=>	Mk\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
RKO	NInBeYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1PINGlEPTB;MUG3Mlg6QCEQvF2=	M4q0clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
ATN-1	M3XHeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MXPJR\|UxRTFzNz64Nlgh\|ryP	M{nZ[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
OVCAR-8	Mo\uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MUTJR\|UxRTFzNz62Olgh\|ryP	NUfDNFFERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
HCC1599	MmPuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MVjJR\|UxRTFzNz6xOVYh\|ryP	NEf1Tmk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
Calu-6	NHyxe5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NVe0Wm9uUUN3ME2xNVYvPzl6IN88US=>	NWLrS4NSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
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LB1047-RCC	Mn:0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M3T0WmlEPTB;OT65PVc6QCEQvF2=	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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LB996-RCC	NIPFVoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M{HTS2lEPTB;OT61NlM6PyEQvF2=	NWLUWYNrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
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NCI-H64	NHHr[3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M3L5VGlEPTB;OT60NlI2PSEQvF2=	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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MKN28	NUHy[YVPT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M1PORWlEPTB;OD64PFUyPiEQvF2=	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
CAL-72	MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NWe3b\|JJUUN3ME24Mlc3OTF\|IN88US=>	NIfab4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
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MC116	MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				Mn;yTWM2OD15LkK4OlkyKM7:TR?=	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
RT-112	NVTSfVVZT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M4q0RWlEPTB;Nz6xOlQ2PiEQvF2=	MoHqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
CAL-85-1	NY\4SVdQT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NFr3e3NKSzVyPU[uOlA6OzZizszN	MoG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
BB65-RCC	NIe5SnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MV3JR\|UxRTZwNUOxPVUh\|ryP	NFPQVoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
CGTH-W-1	NGS0XW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MnW5TWM2OD14LkSxO{DPxE1?	M2PoPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
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D-392MG	M4LHfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NGPwUnVKSzVyPUWuOFkyPDVizszN	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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SW1710	NH\XPIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NH;1RYRKSzVyPUWuOFI1PTZizszN	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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RCC10RGB	NYryO\|Z{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NFzQ[oVKSzVyPUSuNlk1PzlizszN	Moi3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
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OE19	M{fGcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MX3JR\|UxRTNwOU[0OVEh\|ryP	Ml\pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
TE-15	M33zfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NISwUFNKSzVyPUOuPVU{PDNizszN	NIrJTVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
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HCC1569	NEezWnBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NXPofGd[UUN3ME2zMlQ5OzB3IN88US=>	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NVIyPDJxJ{7TRW5ITVJ:L3G+
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COLO-829	MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				Ml\mTWM2OD1{Lke2OVQh\|ryP	MmXnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
RPMI-7951	NYPp[ZJOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M3ezUWlEPTB;Mj63NFE6OyEQvF2=	NYLNXpF6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
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NCI-H2122	Mn3oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NY[zO\|R3UUN3ME2yMlI4Pjh2IN88US=>	MnrsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFEzOTR{Lze+V2FPT0WUPD;hQi=>
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S-117	NYrnfHl4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MnnNTWM2OD1yLkmyNVMyKM7:TR?=	M{PVO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
D-336MG	NXX0Z4ZrT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MoDCTWM2OD1yLki0OlIh\|ryP	NVHXfGM5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
LOXIMVI	NUjjPFd5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M131emlEPTB;MD61PFg{OiEQvF2=	NXPGR5RIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
769-P	M1TnTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MYnJR\|UxRTBwNUi0PVkh\|ryP	M3\oNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQyOjF2Mj:nQnNCVkeHUkyvZV4>
GCT	M321cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M4qwU2lEPTB;MD6zNFY4OiEQvF2=	NFLaOYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OTJzNEKvK\|5USU6JRWK8M4E,
SW982	M3y5V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NFzVfo9KSzVyPUCuNlE1OTZizszN	NWXLcG1DRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDF{MUSyM{c,W0GQR1XSQE9iRg>?
ES-CCE	MYnGeY5kfGmxbjDBd5NigQ>?	NGPRfGw2yqEQvF2=	NUjHU2o4OyCm	MVPEUXNQ	MkW2V5Rzd26pbImgbY5lfWOnczD0bIUh[WO2aY\peJkhd2ZiV370M4JmfGFvY3H0[Y5qdiCyYYToe4F6	NInTcGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe3PVM3PSd-MkS3O\|k{PjV:L3G+
ES-D3	MnzmSpVv[3Srb36gRZN{[Xl?	MoTDOeKh\|ryP	NHG4clQ{KGR?	NGj0NlZFVVOR	MmLCV5Rzd26pbImgbY5lfWOnczD0bIUh[WO2aY\peJkhd2ZiV370M4JmfGFvY3H0[Y5qdiCyYYToe4F6	M175SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{e5N\|Y2Lz5{NEe3PVM3PTxxYU6=
ES-CCE	NYDyZ202S3m2b4TvfIlkKEG\|c3H5	M2rWT\|ExyqEQvF2=	MVmzJIQ>	NEfSdYdFVVOR	NITIcIhKdmS3Y3XzJI1w\GW{YYTlJIN6fG:2b4jpZ4l1gQ>?	NU\ZTlRyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3O\|k{PjVpPkK0O\|c6OzZ3PD;hQi=>
ES-D3	MU\DfZRwfG:6aXOgRZN{[Xl?	NFjPOo8yOMLizszN	MonFN{Bl	M3n0fGROW09?	MmjsTY5lfWOnczDtc4RmemG2ZTDjfZRwfG:6aXPpeJkhf2m2aDDJR\|UxKG:oIESuPUDPxE1?	MoXjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5N{mzOlUoRjJ2N{e5N\|Y2RC:jPh?=
Neural progenitor cells	M3\BPWZ2dmO2aX;uJGF{e2G7	NV3BO45DOyEQvF2=	NUTFPXRIOTBiZB?=	NFLvWVVFVVOR	NXf0WmpjUW6mdXPld{Bo\W6ncnH0bY9vKG:oIH7leZJidCCycn;n[Y5qfG:{IHPlcIx{	MljGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4M{iwN\|QoRjJ2NkO4NFM1RC:jPh?=
A549	MXrBdI9xfG:\|aYOgRZN{[Xl?	M2DkVFUhyrWP	MV:yOEBp	Mn3OSG1UVw>?	M1r0OWlv[3KnYYPld{Bk\WyuIHHwc5B1d3Orcx?=	NWTCU49bRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlc3QDhpPkK0N\|Y4Pjh6PD;hQi=>
H157	NUG1Z4JOSXCxcITvd4l{KEG\|c3H5	M{jVSVUhyrWP	M4rtSlI1KGh?	M1;MfWROW09?	MVTEc4V{KG6xdDDzbYdvcW[rY3HueIx6KGGoZnXjeEBieG:ydH;zbZM>	MnmyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR\|Nke2PFgoRjJ2M{[3Olg5RC:jPh?=
H460	M4PHTWFxd3C2b4Ppd{BCe3OjeR?=	NGm0d\|A2KML3TR?=	NUXrRYlsOjRiaB?=	M2nWbmROW09?	NYPkZ3Q3SXS2ZX71ZZRmeyClZXzsJIFxd3C2b4Ppdy=>	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4N{[4PEc,OjR\|Nke2PFg9N2F-
H157	NF3ZZY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MXe1JOK2VQ>?	MXuyOEBp	NGXVbpVFVVOR	NYTrW\|RGWHKxbX;0[ZMh[2WubDDwdo9tcW[ncnH0bY9v	Mm\nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR\|Nke2PFgoRjJ2M{[3Olg5RC:jPh?=
H460	M4PvTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NEjKTm42KML3TR?=	MXiyOEBp	NEjxUYZFVVOR	MYXQdo9ud3SnczDj[YxtKHC{b3zp[oVz[XSrb36=	NX3xXHRpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlc3QDhpPkK0N\|Y4Pjh6PD;hQi=>
embryonic stem cells (ESC)	MoPDSpVv[3Srb36gRZN{[Xl?	NI\DRYk{KML3TR?=	M2fsW\|EyKGR?	NX3rbm1{TE2VTx?=	NUHkXm5EWHKxbX;0[ZMhe2WuZj3y[Y5mf2GuIH;mJI5iy6:4ZT30fZBmKG2xdYPlJIVu[nK7b37pZ{B{fGWvIHPlcIx{KCiHU1Op	MkD0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NEOzNVIoRjJ\|OESzN\|EzRC:jPh?=
DN3	MmfjSpVv[3Srb36gRZN{[Xl?	NGC2Rm0yOCEQvF2=	NEW2bnQ6PiCq	MlLHSG1UVw>?	M33Fbmlv[3KnYYPld{Bk\WyuIHTleoVtd3CvZX70JIFv\CCycn;sbYZmemG2aX;u	MoPyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3Mk[5PFkoRjJ\|NUK2PVg6RC:jPh?=
platelets	M2LHWGZ2dmO2aX;uJGF{e2G7	M1T6XlExKM7:TR?=	NWW0dpRJOTVibXnu	NHX4fldFVVOR	NUnCfGtEUW6lcnXhd4V{KHSqcn;tZolvNW2nZHnheIVlKHCuYYTlcIV1KGGpZ4Ll[4F1cW:wIHHu[EBCXFBic3XjdoV1cW:w	NHfoRYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KzPVg4Pyd-MkOyN\|k5Pzd:L3G+
hiPSC-NPs	MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MnzGN-KBkc7:TR?=	NIniUYs4OiCq	MkS2SG1UVw>?	M4jPd2lv[3KnYYPld{Bk\WyuIIDyc4xq\mW{YYTpc47DqA>?	Mm\mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEK2PFcoRjJ{NkSyOlg4RC:jPh?=
INS-1E	M4fMU2Z2dmO2aX;uJGF{e2G7	NWHVT\|RsOi53IN88US=>	NFP6RYIzPCCq	MlHjSG1UVw>?	M2DiV2lv\HWlZYOgZUBz\WS3Y4Tpc44hd2ZiTmCxJI93\XKneIDy[ZN{cW:w	NH\BR4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyO\|cxPCd-MkK0Nlc4ODR:L3G+
PANC1	NYXxZnNSSXCxcITvd4l{KEG\|c3H5	NWHTZXVryqB3IN88US=>	MYm3NkBp	NHPSeXVFVVOR	NGm5SFdVemmpZ3Xyd{BidiCjcH;weI91cWNicnXzdI9ve2V?	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJyMUG4Okc,OjJ{MEGxPFY9N2F-
Endothelial cell(EC)	MYjGeY5kfGmxbjDBd5NigQ>?	NFLQfZU{KM7:TR?=		Ml;ESG1UVw>?	MnPRVJJwdW:2ZYOg[Y5ld3SqZXzpZYwh[2WubDCoSWMqKGSrZn\ldoVvfGmjdHnvci=>	NVjNUINmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwOVIxODZpPkKyNFUzODB4PD;hQi=>
HMC1.2	NXOwZo9wT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NXnnc4RkOzBizszN	MnnYO\|IhcA>?	NFPDe4ZFVVOR	MVjS[YR2[2W\|IHPlcIwhe3W{dnn2ZYw>	NXHtWG46RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwN\|k{ODFpPkKyNFM6OzBzPD;hQi=>
Sca-1+ HPC	M1Sx[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NIHreJMzKM7:TR?=	M2fjUlYh\A>?	MlPpSG1UVw>?	MVzTfY5memerc4TpZ4FtdHlicILvcY91\XNiU3PhMVEsyqCKUFOgdJJwdGmoZYLheIlwdiCjbnSgZ49td267IH\vdo1ifGmxbjD0c4dmfGincjD3bZRpKEWJRh?=	NIXFWpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MU[yOFM1QCd-MkG2NlQ{PDh:L3G+
Oct4/Sox2/Klf4-infected MEFs	M363fWZ2dmO2aX;uJGF{e2G7	M2G1eVExKM7:TR?=	MlTFNlgh\A>?	NHuzUIRFVVOR	NWL3TZhYWHKxbX;0[ZMhVUWIczDy[ZBzd2e{YX3tbY5oKFS{YX7z[JVk\WRiYomgU4N1PCxiU3;4Nkwh[W6mIFvs[lQ>	M2L4[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OEO5NFU2Lz5zOUizPVA2PTxxYU6=
H4IIE	MkLXSpVv[3Srb36gRZN{[Xl?	MnjJNVAh\|ryP	MlqyNlQhcA>?	MXrEUXNQ	M4nQbnJm\3WuYYTld{DPui2lYYTlcolvKGGldHn2bZR6KGGwZDDUTXJGKGOxboThbY5qdmdicILvcY91\XJiYXP0bZZqfHl?	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTN3MEG5OUc,OTV\|NUCxPVU9N2F-
insulin receptor–expressing CHO-IR	NXjHcpBsTnWwY4Tpc44hSXO\|YYm=	M1Xu[lUxOCCwTR?=	NUfWUIVjOzBibXnu	MUfEUXNQ	M4OwfWFkfGm4YYTld{BIdHmlb3flckBUgW62aHHz[UApT1Nr	NVHKPI1TRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK2NFY1QTdpPkGyOlA3PDl5PD;hQi=>
3T3-L1	NGXsNpVHfW6ldHnvckBCe3OjeR?=	NWG4fXhjOyEQvF2=	M{Tle\|QhcA>?	NYnWdHBtTE2VTx?=	NHrrU3ZKdmirYnn0d{BxemWjZHnwc4N6fGViZHnm[oVz\W62aXH0bY9v	M1rCdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MEW1NlAxLz5zMkC1OVIxODxxYU6=
CHO-IR	NYPHeXI6TnWwY4Tpc44hSXO\|YYm=		M{jW[VMxKG2rbh?=		M{i4NGVEPTB;MD63O{DPxE1?	MlrKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyMU[xNlEoRjJ7MEG2NVIyRC:jPh?=
HepG2	NHjXd\|VHfW6ldHnvckBCe3OjeR?=		NGrnPWM{KGh?		MX7FR\|UxRTFwNTFOwG0>	NHXSfng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK2NVAzOyd-MkKyOlExOjN:L3G+

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Cleaved PARP / CD31 / VE-Cadherin / α-SMA / vimentin; PubMed: 30709379 J Exp Clin Cancer Res NCI-H460 cells were co-cultured with HUVECs in a 3D culture system, and then treated with 1 μM CHIR-99021, 10 μM or 20 μM Cisplatin, or a combination of both, for 24 h. Cells were harvested and immunoblotted with anti-cleaved PARP, anti-CD31, anti-VE-cadherin, anti-α-SMA, anti-vimentin, and anti-β-actin antibodies. CTNNB1; PubMed: 23993102 Cell Treatment with the GSK3β inhibitor CHIR-99021 led to increased levels of β-catenin in RKO and HT29 (non-mutant) cells. β-catenin; PubMed: 24165128 J Biol Chem HeLa cells were treated with 5 μm CHIR-99021 for the indicated times. The samples were analyzed by Western blot.	30709379 23993102 24165128
Growth inhibition assay	Cell viability; PubMed: 22039301 J Immunol HMC-1.2 cells were cultured in the absence or presence of GSK3β inhibitor (CHIR 99021, 3–30 µM) and viability cell count was assessed by trypan blue dye exclusion after 24 h, 48 h or 72 h. All data are presented as the mean ± SEM of three independent experiments conducted in triplicate. p < 0.05, p < 0.001* p < 0.0001*** vs vehicle treated control cells.	22039301
Immunofluorescence	SOX10 / EdU; PubMed: 28174705 Cell Mol Gastroenterol Hepatol Incorporation of EdU (white arrowheads) into SOX10+ ENS (enteric nervous system) cells with and without CHIR-99021. Oct3/4; PubMed: 24779365 BMC Res Notes Fluorescence micrographs showing the protein expression of Brachyury/Oct3/4 after a three day differentiation of ES-D3 cells with BIO (0.5 μM), SB-216763 (5 μM), CHIR-99021 (5 μM), or CHIR-98014 (1 μM). Nuclei were counterstained with DAPI (blue). The merge is shown in yellow. Original magnification 400x. Scale bar = 50 μm.	28174705 24779365

In vivo

Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. ^[1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. ^[4]

Protocol

Kinase Assay: ^[1]	- Collapse Cell-free kinase assays: Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl₂, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research: ^[3]	- Collapse Cell lines: INS-1E Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM Incubation Time: 1, or 4 days Method: Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system. (Only for Reference)
Animal Research: ^[1]	- Collapse Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes Dosages: ~48 mg/kg Administration: Orally (Only for Reference)

References

Solubility (25°C)

In vitro	DMSO	93 mg/mL (185.33 mM)
	Ethanol	2 mg/mL (3.98 mM)
	Water	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 5% DMSO+30% PEG 300+ddH2O For best results, use promptly after mixing.	24mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download CHIR-99021 (CT99021) HCl SDF

Molecular Weight	501.8
Formula	C₂₂H₁₈Cl₂N_8.HCl
CAS No.	1797989-42-4
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	CC1=CN=C(N1)C2=CN=C(N=C2C3=C(C=C(C=C3)Cl)Cl)NCCNC4=NC=C(C=C4)C#N.Cl

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Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?
Answer:
The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.
Question 2:
How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?
Answer:
Please change the culture medium each day to add the new comound in it if the duration is more than 72h.
Question 3:
What is the difference between S2924 and S1263? Which one is better for differentiation?
Answer:
S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

GSK-3 Signaling Pathway Map

GSK-3 Inhibitors with Unique Features

Selective GSK-3 Inhibitor

TWS119 : GSK-3β-selective, IC50 of 30 nM.
Most Potent GSK-3 Inhibitor

CHIR-98014 : GSK-3α, IC50=0.65 nM; GSK-3β, IC50=0.58 nM.
GSK-3 Inhibitor in Clinical Trial

Tideglusib : Phase II for Alzheimer's disease.
Newest GSK-3 Inhibitor

AZD1080 : Orally active, brain permeable GSK3 inhibitor, inhibits human GSK3α and GSK3β with K_i of 6.9 nM and 31 nM, respectively.

Related GSK-3 Products

CHIR-99021 (CT99021) ^New

CHIR-99021 (CT99021) is a GSK-3α and GSK-3β inhibitor with IC50 of 10 nM and 6.7 nM, respectively. CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3β compared to CDKs. CHIR99021 functions as a Wnt/β-catenin activator and induces autophagy.
LY2090314 ^New

LY2090314 is a potent GSK-3 inhibitor for GSK-3α/β with IC50 of 1.5 nM/0.9 nM; may improve the efficacy of platinum-based chemotherapy regimens. LY2090314 is highly selective towards GSK3 as demonstrated by its fold selectivity relative to a large panel of kinases.
TDZD-8 ^New

TDZD-8 (NP 01139) is a non-ATP competitive GSK-3β inhibitor with IC50 of 2 μM; minimal inhibitory effect observed on CDK1, casein kinase II, PKA and PKC.
TWS119

TWS119 is a GSK-3β inhibitor with IC50 of 30 nM in a cell-free assay; capable of inducing neuronal differentiation and may be useful to stem cell biology. GSK-3β inhibition triggers autophagy.
SB216763

SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β. SB216763 activates autophagy.
Tideglusib (NP031112)

Tideglusib (NP031112, NP-12) is an irreversible, non ATP-competitive GSK-3β inhibitor with IC50 of 60 nM in a cell-free assay; fails to inhibit kinases with a Cys homologous to Cys-199 located in the active site. Phase 2.
CHIR-98014

CHIR-98014 (CT98014) is a potent GSK-3α/β inhibitor with IC50 of 0.65 nM/0.58 nM in cell-free assays, with the ability to distinguish GSK-3 from its closest homologs Cdc2 and ERK2.
BIO

BIO (GSK-3 Inhibitor IX, 6-bromoindirubin-3-oxime, 6-Bromoindirubin-3'-oxime, MLS 2052) is a specific inhibitor of GSK-3 with IC50 of 5 nM for GSK-3α/β in a cell-free assay, shows >16-fold selectivity over CDK5, also a pan-JAK inhibitor with IC50 of 30 nM for Tyk2. BIO induces apoptosis in human melanoma cells.

Features:The first pharmacological agent shown to maintain self-renewal in human and mouse embryonic stem cells.
AR-A014418

AR-A014418 (GSK-3β Inhibitor VIII) is an ATP-competitive, and selective GSK3β inhibitor with IC50 and K_i of 104 nM and 38 nM in cell-free assays, without significant inhibition on 26 other kinases tested.

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CHIR-99021 (CT99021) HCl