CHIR-99021 (CT99021) HCl

Catalog No.S2924

Molecular Weight(MW): 501.8

CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.

Cited by 73 Publications

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Nat Commun, 2015, 6:7413

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University of GOTHENBURG, 2014, 5495

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Bachelor of Science, 2012, East China Normal University

PubMed:

9 Customer Reviews

Differentiation induced by treatment with the Gsk3 inhibitor in hPSCs is β-catenin dependent. H9-7TGP cells were treated with 12 uM CH in mTeSR1 for 4 d. Immunofluorescent staining for Oct4, Isl1, and Nkx2.5 was compared with GFP expression. (Scale bars, 50 uM.)

Proc Natl Acad Sci U S A 2012 109(27):E1848-57. CHIR-99021 (CT99021) HCl purchased from Selleck.

Gpc4 regulates ESC response to Wnt/β-catenin signaling. (E) Quantitative analysis of control and Gpc4-mutant beating foci exposed to the GSK3 inhibitor, CHIR 99021 (1 and 3 μM, from day 0 to day 5). Analysis was done at different time points. Gpc4- mutant cells treated with CHIR 99021 behave as controls (mean±SEM; n=2).

Stem Cells 2012 30, 1863-1874. CHIR-99021 (CT99021) HCl purchased from Selleck.
Characterization of rES cells. A: image of normal rES cell colonies on feeder layers. B: rES colonies were positive for AP staining. CeE: rES colonies readily expressed pluripotent markers, Sox2 (C), Oct4 (D), and SSEA-1 (E). Blue, DAPI. Scale bars: 100 um.

J Genet Genomics 2012 39, 643e651. CHIR-99021 (CT99021) HCl purchased from Selleck.

Inhibition of GSK3 activity induces an autophagic response in human pancreatic cancer cells. PANC1 cells were treated for 24 h with DMSO, CHIR99021 (CHIR; 5 uM) or the mTOR inhibitor Torin1 (250 nM). Autophagosome (punctate LC3B) (D) and lysosome (E) labeling was visualized with a Zeiss LSM700 confocal microscope. Images were acquired with a 20x (D) or 63x (E) objective. Nuclei were stained with DAPI. Scale bars, 20 祄.

J Biol Chem 2015 290(9), 5592-605 . CHIR-99021 (CT99021) HCl purchased from Selleck.
A. Vero cells were pretreated with the indicated concentrations of CHIR-99021 for 1 h and then infected with PEDV (MOI = 1) for 8 or 10 h. Cell lysates were prepared and analyzed using Western blotting.

PLoS One, 2016, 11(8):e0161508. CHIR-99021 (CT99021) HCl purchased from Selleck.

Smad linker phosphorylation involves GSK3 in human melanoma lines. Inhibition of Smad2 and Smad3 linker phosphorylation in the presence of the specific GSK3 specific inhibitor, CT99021. 24 hours post seeding, WM793 and 1205LU cells were serum-starved for about 16 hours, and incubated in the absence (2) or presence (+) of 2 uM of CT99021 for two hours. Immunoblots were performed as in A. p: Phosphorylated. S: Serine; T: Threonine.

PLoS One 2012 7, e47312. CHIR-99021 (CT99021) HCl purchased from Selleck.
SKOV3/DDP cells were treated with a range of concentration of (A) CHIR-99021 or in combination with 20 µM cisplatin for 48 h.

Exp Ther Med, 2016, 12(6):3851-3858. CHIR-99021 (CT99021) HCl purchased from Selleck.

PANC1 cells were treated with GSK3 inhibitors SB216763 (SB 10µM or SB 20µM) or CHIR99021 (CHIR 5µM) for 72h.

CHIR-99021 (CT99021) HCl purchased from Selleck.
Activity of canonical WNT/ β -CATENIN signals induced by CHIR 99021 in the developing human lung. Human lung tissue explants obtained at 15 weeks of gestation were exposed to 0, 5 and 10 μM CHIR 99021 in vitro for 72 h. (A-B) Western blot analysis showed expression of β -CATENIN in human lung tissues. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (C) qRT-PCR analysis detected a dose-dependent increase in WNT signaling transcription factors and target genes following exposure to CHIR 99021. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (D-K) Histology examination showed obvious morphogenetic changes of the airway epithelial cells associated with CHIR 99021 treated lung tubes. Scale bars: D-G =100 μm; H-K = 20 μm.

CHIR-99021 (CT99021) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description

Targets

GSK-3β ^[1] (Cell-free assay)	GSK-3α ^[1] (Cell-free assay)
6.7 nM	10 nM

In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. ^[1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). ^[2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. ^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
3T3-L1	NXvpcVdmTnWwY4Tpc44hSXO\|YYm=	NXTRPVNzOyEQvF2=	MljWOEBp	MWnEUXNQ	NIDsfpdKdmirYnn0d{BxemWjZHnwc4N6fGViZHnm[oVz\W62aXH0bY9v	M1jtblEzODV3MkCw
insulin receptor–expressing CHO-IR	Mnu1SpVv[3Srb36gRZN{[Xl?	MmnWOVAxKG6P	NFHtXVE{OCCvaX6=	NGX3TXpFVVOR	MYjBZ5RqfmG2ZYOgS4x6[2:pZX6gV5lvfGijc3WgLGdUMQ>?	NGnW[2IyOjZyNkS5Oy=>
H4IIE	MnLkSpVv[3Srb36gRZN{[Xl?	NG\YRW0yOCEQvF2=	NV\XXIdTOjRiaB?=	NHe5VnFFVVOR	MYHS[Yd2dGG2ZYOg{tIu[2G2ZX7pckBi[3Srdnn0fUBidmRiVFnSSUBkd262YXnubY5oKHC{b33veIVzKGGldHn2bZR6	MoTzNVU{PTBzOUW=
Oct4/Sox2/Klf4-infected MEFs	NGLtUHpHfW6ldHnvckBCe3OjeR?=	MmjMNVAh\|ryP	MVmyPEBl	MWnEUXNQ	NXftNHlNWHKxbX;0[ZMhVUWIczDy[ZBzd2e{YX3tbY5oKFS{YX7z[JVk\WRiYomgU4N1PCxiU3;4Nkwh[W6mIFvs[lQ>	NYrIUoZHOTl6M{mwOVU>
Sca-1+ HPC	M2TlRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MnLVNkDPxE1?	MmLFOkBl	NUP2SFNSTE2VTx?=	MkLpV5lv\XKpaYP0bYNidGy7IIDyc41wfGW\|IGPjZU0yM8LiSGDDJJBzd2yrZnXyZZRqd25iYX7kJINwdG:weTDmc5Ju[XSrb36geI9o\XSqZYKge4l1cCCHR1[=	NYf3NXZNOjF4MkSzOFg>
HMC1.2	Mnj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NYSxdGd4OzBizszN	MmnPO\|IhcA>?	MVXEUXNQ	Mn31VoVlfWOnczDj[YxtKHO3co\peoFt	M{XMb\|IzODN7M{Cx
Endothelial cell(EC)	M4[3XWZ2dmO2aX;uJGF{e2G7	MYGzJO69VQ>?		NHnOO\|NFVVOR	MV;Qdo9ud3SnczDlcoRwfGinbHnhcEBk\WyuIDjFR{kh\GmoZnXy[Y51cWG2aX;u	Mn7TNlIxPTJyME[=
PANC1	NVuzb5RoSXCxcITvd4l{KEG\|c3H5	MmfGxsA2KM7:TR?=	NFv4T\|Q4OiCq	MV;EUXNQ	NWPxTXFbXHKrZ3fldpMh[W5iYYDvdJRwfGmlIILld5BwdnOn	MUWyNlIxOTF6Nh?=
INS-1E	M1rkTWZ2dmO2aX;uJGF{e2G7	M2PUWFIvPSEQvF2=	NUHZRWRqOjRiaB?=	NGW0UZpFVVOR	NWrxS\|R6UW6mdXPld{BiKHKnZIXjeIlwdiCxZjDOVFEhd3[ncnX4dJJme3Orb36=	NEnTW3AzOjR{N{ewOC=>
hiPSC-NPs	M4nYRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NYTG[GZNO+LCid88US=>	Ml\rO\|IhcA>?	M3HWbGROW09?	MknuTY5kemWjc3XzJINmdGxicILvcIln\XKjdHnvcuKh	MoLvNlI3PDJ4OEe=
platelets	NEe2cpZHfW6ldHnvckBCe3OjeR?=	MkLGNVAh\|ryP	M{OzVVE2KG2rbh?=	M4XtTmROW09?	M3mzPGlv[3KnYYPld{B1cHKxbXLpck1u\WSrYYTl[EBxdGG2ZXzleEBi\2e{ZXfheIlwdiCjbnSgRXRRKHOnY4LleIlwdg>?	NXf6cJloOjN{M{m4O\|c>
DN3	NGXySWRHfW6ldHnvckBCe3OjeR?=	M1;0bFExKM7:TR?=	NXu5dXBFQTZiaB?=	M3TWZmROW09?	NVniN5BQUW6lcnXhd4V{KGOnbHyg[IV3\WyxcH3lcpQh[W6mIIDyc4xq\mW{YYTpc44>	M37jcFI{PTJ4OUi5
embryonic stem cells (ESC)	M2DoWWZ2dmO2aX;uJGF{e2G7	NVnRVXV3OyEEtV2=	NIrNPW8yOSCm	M1rTUWROW09?	M4fVV3Bzd22xdHXzJJNmdGZvcnXu[ZdidCCxZjDuZeOwfmVvdInw[UBud3W\|ZTDlcYJzgW:waXOgd5RmdSClZXzsd{ApTVOFKR?=	MV:yN\|g1OzNzMh?=
H460	NIHJbldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NIrsOlA2KML3TR?=	M1rnTlI1KGh?	MX\EUXNQ	MVHQdo9ud3SnczDj[YxtKHC{b3zp[oVz[XSrb36=	MljoNlQ{Pjd4OEi=
H157	NIf2N2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NFewVIs2KML3TR?=	M13aTlI1KGh?	MULEUXNQ	MoTiVJJwdW:2ZYOgZ4VtdCCycn;sbYZmemG2aX;u	NGCyfZAzPDN4N{[4PC=>
H460	NFGybpVCeG:ydH;zbZMhSXO\|YYm=	MlXTOUDDvU1?	NGPVRogzPCCq	NFrOT\|dFVVOR	NVewR\|RbSXS2ZX71ZZRmeyClZXzsJIFxd3C2b4Ppdy=>	NEDrWIQzPDN4N{[4PC=>
H157	M17IZ2Fxd3C2b4Ppd{BCe3OjeR?=	NXyySFhtPSEEtV2=	MXmyOEBp	MoL0SG1UVw>?	MYTEc4V{KG6xdDDzbYdvcW[rY3HueIx6KGGoZnXjeEBieG:ydH;zbZM>	NGW5V\|UzPDN4N{[4PC=>
A549	MX\BdI9xfG:\|aYOgRZN{[Xl?	M{DsZlUhyrWP	NH;6T20zPCCq	MmjFSG1UVw>?	NFrqNWxKdmO{ZXHz[ZMh[2WubDDhdI9xfG:\|aYO=	MmXCNlQ{Pjd4OEi=
Neural progenitor cells	M3;NWWZ2dmO2aX;uJGF{e2G7	NEX2WIs{KM7:TR?=	MWmxNEBl	M{XNW2ROW09?	MVHJcoR2[2W\|IHflcoVz[XSrb36gc4YhdmW3cnHsJJBzd2enbnn0c5Ih[2WubIO=	M2fydVI1PjN6MEO0
ES-D3	M3K3eGN6fG:2b4jpZ{BCe3OjeR?=	MXKxNOKh\|ryP	MYCzJIQ>	MlexSG1UVw>?	M3HhSWlv\HWlZYOgcY9l\XKjdHWgZ5l1d3SxeHnjbZR6KHerdHigTWM2OCCxZjC0Mlkh\|ryP	NVHnTW82OjR5N{mzOlU>
ES-CCE	NEXndJhEgXSxdH;4bYMhSXO\|YYm=	MofzNVDDqM7:TR?=	M4DGflMh\A>?	MnvXSG1UVw>?	NIfGeXdKdmS3Y3XzJI1w\GW{YYTlJIN6fG:2b4jpZ4l1gQ>?	NUfBbJRPOjR5N{mzOlU>
ES-D3	MmDJSpVv[3Srb36gRZN{[Xl?	MVy1xsDPxE1?	MU[zJIQ>	MnT5SG1UVw>?	NX;ZN3k3W3S{b37ncJkhcW6mdXPld{B1cGViYXP0bZZqfHlib3[gW451N2KndHGtZ4F1\W6rbjDwZZRpf2G7	MUCyOFc4QTN4NR?=
ES-CCE	M1rhUmZ2dmO2aX;uJGF{e2G7	NV62N2J2PcLizszN	NV\jSnhHOyCm	NFfIRVRFVVOR	M33WPHN1em:wZ3z5JIlv\HWlZYOgeIhmKGGldHn2bZR6KG:oIGfueE9j\XSjLXPheIVvcW5icHH0bJdigQ>?	MVGyOFc4QTN4NR?=
SW982	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M4\W[mlEPTB;MD6yNVQyPiEQvF2=	MUHTRW5ITVJ?
GCT	NITsVlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M3zTOmlEPTB;MD6zNFY4OiEQvF2=	NFTHNJJUSU6JRWK=
769-P	M{jsdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NY\neGhNUUN3ME2wMlU5PDl7IN88US=>	NIjzdXFUSU6JRWK=
LOXIMVI	NHTyU5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MkLpTWM2OD1yLkW4PFMzKM7:TR?=	MVHTRW5ITVJ?
D-336MG	MlToS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NEfybWVKSzVyPUCuPFQ3OiEQvF2=	MYjTRW5ITVJ?
S-117	NIX5XYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MVjJR\|UxRTBwOUKxN\|Eh\|ryP	NGfyco5USU6JRWK=
D-247MG	MnHyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NYPRT41jUUN3ME2wMlk1QDV{IN88US=>	MnvHV2FPT0WU
TYK-nu	NXnmRoV[T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NGrCT4lKSzVyPUGuNFc6OTJizszN	MoG4V2FPT0WU
A498	M3X0fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M{DsWGlEPTB;MT6zNFQ3QSEQvF2=	NXLHR5k2W0GQR1XS
BCPAP	NIDiTIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1jpVGlEPTB;MT62Olg6QCEQvF2=	MWLTRW5ITVJ?
KYSE-180	Mm[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MWHJR\|UxRTFwN{K3PFgh\|ryP	M134RXNCVkeHUh?=
MIA-PaCa-2	NFS2fZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NYDUPWhEUUN3ME2xMlc2QTd5IN88US=>	NFrUU\|RUSU6JRWK=
HCC1395	MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MX\JR\|UxRTFwN{iyO\|Mh\|ryP	NGi3[I1USU6JRWK=
RS4-11	NUfxbW5MT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				Mn\ZTWM2OD1zLkm1OFE2KM7:TR?=	MVTTRW5ITVJ?
NCI-H2122	NI\DWYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M3zYfWlEPTB;Mj6yO\|Y5PCEQvF2=	NFe4cJRUSU6JRWK=
SNU-423	NGrOcXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NHvZd4FKSzVyPUKuOFk4QDdizszN	MVLTRW5ITVJ?
SK-LMS-1	MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NWnY[FY2UUN3ME2yMlY1OTJ6IN88US=>	MnHpV2FPT0WU
RPMI-7951	M2m2ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MnrpTWM2OD1{LkewNVk{KM7:TR?=	NITISGNUSU6JRWK=
COLO-829	NGm5ZmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MmfrTWM2OD1{Lke2OVQh\|ryP	NFXpNlFUSU6JRWK=
SCC-9	MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M{LrT2lEPTB;Mj65OVQ5PSEQvF2=	M1;wN3NCVkeHUh?=
A704	NGjFb3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MmfiTWM2OD1\|LkC4NVIh\|ryP	MX7TRW5ITVJ?
HOP-62	NFPHeWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NWPZTXdMUUN3ME2zMlM3ODV2IN88US=>	M3f4NXNCVkeHUh?=
HCC1569	NX;TUGRiT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NVzqdmk{UUN3ME2zMlQ5OzB3IN88US=>	Mk\rV2FPT0WU
A172	M{jFWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M4TncmlEPTB;Mz63OVQ6QCEQvF2=	M4jlWnNCVkeHUh?=
MOLT-16	NIH0XJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NV:5e5l[UUN3ME2zMlg5PTV4IN88US=>	M3\rOHNCVkeHUh?=
TE-15	M1\UdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M1LV[2lEPTB;Mz65OVM1OyEQvF2=	NHvXO4pUSU6JRWK=
OE19	MnjaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NHTpOZBKSzVyPUOuPVY1PTFizszN	M3yy[nNCVkeHUh?=
MHH-ES-1	MmPES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MkP6TWM2OD12LkC0OVQ2KM7:TR?=	MWLTRW5ITVJ?
NKM-1	NIPvcWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				Ml7WTWM2OD12LkGzPVcyKM7:TR?=	MlLZV2FPT0WU
RCC10RGB	M1flSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MlrNTWM2OD12LkK5OFc6KM7:TR?=	Mk\XV2FPT0WU
BxPC-3	M{Dkbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MXTJR\|UxRTRwN{CzPVEh\|ryP	NXix[5YzW0GQR1XS
ALL-PO	NWns[JE1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MYXJR\|UxRTRwOEO4OVgh\|ryP	MV7TRW5ITVJ?
SK-OV-3	Ml\KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MUjJR\|UxRTRwOUmzOEDPxE1?	MoHUV2FPT0WU
SW1710	NHXEOndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MV7JR\|UxRTVwNEK0OVYh\|ryP	MXHTRW5ITVJ?
NCI-H2452	MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M32wOGlEPTB;NT60OVYzQCEQvF2=	MUTTRW5ITVJ?
D-392MG	NHO2[oJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NGPCOWJKSzVyPUWuOFkyPDVizszN	MYfTRW5ITVJ?
LB2518-MEL	M3XPUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NH62blBKSzVyPUWuO\|I3OjdizszN	NYnjT2ZuW0GQR1XS
BL-41	NYfxPFdjT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M32xR2lEPTB;NT64NVI3PiEQvF2=	NVHyTHBzW0GQR1XS
WM-115	MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NYq1T4VuUUN3ME21MlgyPTB7IN88US=>	MkX0V2FPT0WU
SK-MEL-2	MnLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NHTHd3JKSzVyPU[uN\|g{PDRizszN	NHjFTmNUSU6JRWK=
CGTH-W-1	MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M2myOGlEPTB;Nj60NVch\|ryP	MmTXV2FPT0WU
BB65-RCC	MljBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M4fvSWlEPTB;Nj61N\|E6PSEQvF2=	MoexV2FPT0WU
CAL-85-1	MnLmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MX7JR\|UxRTZwNkC5N\|Yh\|ryP	M4LTbnNCVkeHUh?=
RT-112	NWLo[5Q3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NVi4d5pTUUN3ME23MlE3PDV4IN88US=>	NUG1cmpVW0GQR1XS
MC116	Mo[zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NF24UFNKSzVyPUeuNlg3QTFizszN	M1XDTHNCVkeHUh?=
G-361	MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MmTMTWM2OD15LkS2OVYyKM7:TR?=	NH[4S4RUSU6JRWK=
SW1417	NVLI[JN4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NYrTXIJXUUN3ME23MlY1PTh2IN88US=>	NEnpToNUSU6JRWK=
MV-4-11	NVXuVGpZT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NXT5d2Y6UUN3ME24MlU1OzB2IN88US=>	NY\oUoE5W0GQR1XS
CAL-72	M4PBXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NH21UIZKSzVyPUiuO\|YyOTNizszN	MUDTRW5ITVJ?
MKN28	NF7Td21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M{XiOWlEPTB;OD64PFUyPiEQvF2=	NGXiPWFUSU6JRWK=
MEG-01	MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M2S3[mlEPTB;OD65PVA1OyEQvF2=	M3n4NXNCVkeHUh?=
ACHN	M1X1bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MknNTWM2OD17LkOwOVQ3KM7:TR?=	MnnwV2FPT0WU
NCI-H1755	NWXOWHdrT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NV[3No52UUN3ME25MlM6QTh4IN88US=>	NYPMNJNmW0GQR1XS
NCI-H64	MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MkPZTWM2OD17LkSyNlU2KM7:TR?=	MlrRV2FPT0WU
COLO-205	MmnSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M1rPVGlEPTB;OT60N\|gzOSEQvF2=	NYfuWlJHW0GQR1XS
CTV-1	NVHoRpQ2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NHOzSmxKSzVyPUmuOFM5QTRizszN	MU\TRW5ITVJ?
U-2-OS	NW\OR2xNT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NX6wZpR[UUN3ME25MlQ2ODB4IN88US=>	NG\N[IRUSU6JRWK=
LB996-RCC	NUjEcVhzT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NHH1c25KSzVyPUmuOVI{QTdizszN	M4\KXXNCVkeHUh?=
SK-N-DZ	MkXGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MYLJR\|UxRTlwNkW3OFIh\|ryP	MVvTRW5ITVJ?
EFM-19	NHG1OYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NXv5V4M{UUN3ME25Mlc2PTZ4IN88US=>	NUL4OFJpW0GQR1XS
DBTRG-05MG	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M4DSNWlEPTB;OT63O\|c4KM7:TR?=	MmTiV2FPT0WU
U-118-MG	Mny2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M2nGOmlEPTB;OT65OFQ1PCEQvF2=	M2r1Z3NCVkeHUh?=
LB1047-RCC	MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MU\JR\|UxRTlwOUm3PVgh\|ryP	M4i0bnNCVkeHUh?=
NCI-H28	NFrEV\|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NFLjNFhKSzVyPUGwMlQ1OTNizszN	MWHTRW5ITVJ?
MZ2-MEL	NEPZZoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MWLJR\|UxRTFyLkS2O\|ch\|ryP	NGX5dZVUSU6JRWK=
M059J	NGLGTGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MVjJR\|UxRTFyLk[xNFIh\|ryP	M2PMOHNCVkeHUh?=
HT-3	NIfNOFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M3LQR2lEPTB;MUCuOlE4QSEQvF2=	M2HJdXNCVkeHUh?=
SNU-387	NXy4dJZIT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MV;JR\|UxRTFyLkeyN\|Uh\|ryP	NGqzcIdUSU6JRWK=
NCI-H526	NFLUdlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M2izSmlEPTB;MUGuNVM3PSEQvF2=	NGXRPFRUSU6JRWK=
HEL	Mlu2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MnG3TWM2OD1zMT62OVc6KM7:TR?=	M3nnT3NCVkeHUh?=
PA-1	MlHsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NHOwUVJKSzVyPUGxMlk{KM7:TR?=	NIXGdpdUSU6JRWK=
PC-14	NEGxUFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1q0WGlEPTB;MUKuN\|I1KM7:TR?=	NFjxRYhUSU6JRWK=
C8166	NXXK[XNST3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				Mn7mTWM2OD1zMz6yN\|E5KM7:TR?=	NES4SGZUSU6JRWK=
NCI-H82	Mn\mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MYHJR\|UxRTF\|LkK5NFkh\|ryP	NFT6O3pUSU6JRWK=
DSH1	Mor2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MXvJR\|UxRTF\|LkO4O\|kh\|ryP	NInRcZhUSU6JRWK=
T98G	MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NFy5SVNKSzVyPUGzMlQ4OTdizszN	NXPCNWsyW0GQR1XS
KU812	NGj6[GNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NVnNXWE2UUN3ME2xN{42OTd3IN88US=>	MoTiV2FPT0WU
SW48	MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M4[0RmlEPTB;MUOuO\|I3PSEQvF2=	MkLJV2FPT0WU
MEL-HO	NXXTdmNMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NIHaNIhKSzVyPUGzMlg5OjlizszN	NXfaeYFJW0GQR1XS
SF539	NWDyV\|g{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NE\aNIhKSzVyPUG0MlAyOTZizszN	NUfWbWhKW0GQR1XS
NB1	MlXTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NWXzWGpGUUN3ME2xOE4yQTN3IN88US=>	MUHTRW5ITVJ?
ST486	MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MV7JR\|UxRTF2LkKyOVEh\|ryP	NV7Hem01W0GQR1XS
LoVo	NVfOWnBrT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MW\JR\|UxRTF3LkK2OVkh\|ryP	MUXTRW5ITVJ?
NH-12	M3LD[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MYnJR\|UxRTF4LkCwPVch\|ryP	NFTwV29USU6JRWK=
CCRF-CEM	NGezblVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NIrp[plKSzVyPUG2MlY4ODJizszN	MWHTRW5ITVJ?
Daudi	MkPpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NIjqOJpKSzVyPUG3MlYyPDlizszN	NYnrRVV2W0GQR1XS
SIMA	NXuxelZoT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NVL3WphjUUN3ME2xO{46OTF6IN88US=>	NGW1VHBUSU6JRWK=
8305C	NHTBbJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MnfTTWM2OD1zOD61N\|A2KM7:TR?=	MYTTRW5ITVJ?
EM-2	M2fofWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NIjlSVBKSzVyPUG4MlYzPDhizszN	NUnMe4RMW0GQR1XS
SNU-449	MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MWHJR\|UxRTF6LkexOUDPxE1?	MlvyV2FPT0WU
A101D	M2HKdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NHK2PG5KSzVyPUG5MlI1OyEQvF2=	MU\TRW5ITVJ?
MHH-NB-11	NYnQT4UyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MYPJR\|UxRTF7LkWzOFIh\|ryP	M1jBXHNCVkeHUh?=
Becker	NFnPS2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NX[2So1mUUN3ME2yNE4yPzd5IN88US=>	MofiV2FPT0WU
NCI-H727	MkDvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MoPWTWM2OD1{MD6yNVY6KM7:TR?=	NE\sW4JUSU6JRWK=
HCC1187	NWDCVXcxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NXrG[HJTUUN3ME2yNE4{PTh7IN88US=>	NX3WdVgxW0GQR1XS
KYSE-450	M3nHWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NF7hO5RKSzVyPUKxMlU{PTRizszN	NYT5XWQ2W0GQR1XS
SK-MEL-28	NEP3cXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MUfJR\|UxRTJzLk[4OlMh\|ryP	NXT6[XdUW0GQR1XS
BPH-1	MnG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NGXifWhKSzVyPUKxMlcxOzVizszN	NUjydI01W0GQR1XS
EoL-1-	MlTHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M4\i[WlEPTB;MkGuPFM5PSEQvF2=	NX;GZ4d7W0GQR1XS
TGBC24TKB	MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MkLGTWM2OD1{MT64PVU4KM7:TR?=	MWPTRW5ITVJ?
KARPAS-299	M1PMR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NVXxSlNnUUN3ME2yNk4yQTV2IN88US=>	NYDaZZN3W0GQR1XS
SW684	M{jZVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NFrLUpdKSzVyPUKyMlI5PThizszN	MVHTRW5ITVJ?
RXF393	NUPoUWJjT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MYrJR\|UxRTJ{Lk[wOlgh\|ryP	NWHmWXJmW0GQR1XS
LS-513	M3jpcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MW\JR\|UxRTJ{Lk[xPVIh\|ryP	M4HDd3NCVkeHUh?=
KURAMOCHI	NVyzZYNPT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MWTJR\|UxRTJ{LkewOlkh\|ryP	MXrTRW5ITVJ?
GI-1	MmDTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NYrDWHUyUUN3ME2yNk44QDRzIN88US=>	Mn2yV2FPT0WU
M14	NWT2cng2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NEHHcItKSzVyPUKzMlEyOzJizszN	Mn;OV2FPT0WU
REH	NUDh[Ip[T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NVnxSW1QUUN3ME2yN{4yPjl7IN88US=>	NXfqZ5A3W0GQR1XS
K5	MnjxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NHm1S2RKSzVyPUKzMlU1QDZizszN	MVrTRW5ITVJ?
PANC-10-05	NHj4bVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NVjOcGNDUUN3ME2yOE4xQDl6IN88US=>	NYDsS4FIW0GQR1XS
HMV-II	MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M{fJ[2lEPTB;MkSuNlE3OyEQvF2=	MV;TRW5ITVJ?
NCI-H1963	NFHCV2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NYSyUVF4UUN3ME2yOE4zQDN7IN88US=>	NHu3VnpUSU6JRWK=
LU-139	NIXVcIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MXvJR\|UxRTJ2LkizOUDPxE1?	NUDUWmZIW0GQR1XS
KASUMI-1	NUjPNlIxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NFjm[W5KSzVyPUK0Mlk{OjhizszN	NFX6bpBUSU6JRWK=
RO82-W-1	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MmrNTWM2OD1{Nj63N\|c4KM7:TR?=	NHjKOJhUSU6JRWK=
NCI-H1770	NYPoNopYT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NXvzTVNwUUN3ME2yOk45QTh4IN88US=>	NF60[ZRUSU6JRWK=
SNG-M	MkTWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MorqTWM2OD1{Nz6yNlcyKM7:TR?=	NYHLb5BPW0GQR1XS
KLE	Mn;ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NYDEN\|diUUN3ME2yO{4zPTZ3IN88US=>	Mn7HV2FPT0WU
NCI-H2170	MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MXPJR\|UxRTJ5LkWyPFIh\|ryP	NF7STGlUSU6JRWK=
COLO-792	Mn7pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NUnJVY1qUUN3ME2yO{44PzJ6IN88US=>	M3P6RnNCVkeHUh?=
ES8	MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MlTjTWM2OD1{Nz63PFE1KM7:TR?=	M2DIbXNCVkeHUh?=
MC-CAR	MmXBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MnH3TWM2OD1{Nz63PVA4KM7:TR?=	MU\TRW5ITVJ?
SU-DHL-1	NUfjO211T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MU\JR\|UxRTJ5Lkm0O\|Qh\|ryP	NYfEbVh2W0GQR1XS
OAW-28	MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MnjXTWM2OD1{OD6wNFUzKM7:TR?=	Mm[zV2FPT0WU
C32	M4fPfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MmHZTWM2OD1{OD6wPFI3KM7:TR?=	M2P2fHNCVkeHUh?=
SW872	NYW3dFdsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MkHyTWM2OD1{OD61N\|I3KM7:TR?=	MnXHV2FPT0WU
JiyoyeP-2003	NFnTV5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NIXGOG1KSzVyPUK5MlY4OTlizszN	M3\MS3NCVkeHUh?=
A204	NH7sc4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MWHJR\|UxRTJ7Lk[4Nlgh\|ryP	NEHvc2NUSU6JRWK=
IST-MES1	M2HyW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NIXIfY1KSzVyPUK5MlcxQTJizszN	NF7QWXlUSU6JRWK=
LCLC-103H	M3T4cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M{e4N2lEPTB;M{CuNlA2QSEQvF2=	NFv3WVdUSU6JRWK=
CAKI-1	NG\XWVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NUfIfItCUUN3ME2zNE4zPjV4IN88US=>	NVzFO3ZtW0GQR1XS
NALM-6	MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M4C0WWlEPTB;M{CuOFk3PSEQvF2=	MUnTRW5ITVJ?
SK-MM-2	MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				Mo\2TWM2OD1\|MD65O\|kzKM7:TR?=	M3rlUXNCVkeHUh?=
LB373-MEL-D	NUD5UnlMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M1HqemlEPTB;M{GuNFA1PyEQvF2=	NHjF[3RUSU6JRWK=
CAS-1	MoH6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M4HCdWlEPTB;M{GuO\|Y3PSEQvF2=	M1XYXnNCVkeHUh?=
KNS-42	M1z2OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M1rqfGlEPTB;M{GuPFY3OiEQvF2=	MYnTRW5ITVJ?
SW948	NFzzSWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NFP4[5dKSzVyPUOyMlc2PDhizszN	M4TIenNCVkeHUh?=
HCC1143	M3:0dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NETjeJRKSzVyPUOyMlc6PzlizszN	M3rkc3NCVkeHUh?=
NCI-H1648	MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M1vDcGlEPTB;M{KuPFQ3PyEQvF2=	MlXOV2FPT0WU
RVH-421	NVTiTGE1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M3jKdWlEPTB;M{OuNVY5KM7:TR?=	NFHF[25USU6JRWK=
KU-19-19	NETTd2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NWq4RYNQUUN3ME2zN{44PTN2IN88US=>	M3;VSXNCVkeHUh?=
PFSK-1	NIPLcVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NXTre2d4UUN3ME2zOE46OjRzIN88US=>	NGT3TXBUSU6JRWK=
TK10	NIHhNItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MmrsTWM2OD1\|NT6xN\|IzKM7:TR?=	NHy5O4dUSU6JRWK=
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NCI-H2405	MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NUnPR5JSUUN3ME21Ok4yPjl\|IN88US=>	MVvTRW5ITVJ?
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NCI-H1703	Ml63S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M3KxdGlEPTB;NkiuNVE4OSEQvF2=	NWnJcXA2W0GQR1XS
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KALS-1	MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MUHJR\|UxRTd\|LkW0O\|Uh\|ryP	MmrSV2FPT0WU
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8-MG-BA	NGnWNXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M2rPc2lEPTB;N{[uNVA6PiEQvF2=	MoPyV2FPT0WU
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PANC-03-27	NH3hTZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M2n0O2lEPTB;N{iuOFM2KM7:TR?=	NE\WbmZUSU6JRWK=
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HCE-T	NFWzbWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MlyyTWM2OD16MD6xOVg5KM7:TR?=	MmXQV2FPT0WU
KYSE-410	MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NE\nOmZKSzVyPUiwMlI5PTdizszN	MWLTRW5ITVJ?
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NCI-H1417	MkXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M{HP[GlEPTB;OEGuOFM3QCEQvF2=	NUGydIt2W0GQR1XS
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NCI-H650	MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MXjJR\|UxRTh7Lki3NlQh\|ryP	M1LUdXNCVkeHUh?=
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Mewo	Mlm4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MY\JR\|UxRTl7LkW2NFMh\|ryP	MWTTRW5ITVJ?
LU-65	M{TW[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NFjjSGFKSzVyPUGwNU4xQDFizszN	M3rpZ3NCVkeHUh?=
Saos-2	MnPYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M135V2lEPTB;MUCxMlQ{PyEQvF2=	MXzTRW5ITVJ?
TE-11	M4\tNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NYLEdXBTUUN3ME2xNFEvPzJ2IN88US=>	NIPOfmNUSU6JRWK=
SK-MES-1	MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MU\JR\|UxRTFyMT63OlIh\|ryP	MUDTRW5ITVJ?
KE-37	NXLVVnQzT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MYXJR\|UxRTFyMj65OUDPxE1?	NWXodmxTW0GQR1XS
MKN45	MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NX2xRlhEUUN3ME2xNFMvPTh2IN88US=>	M{LRfHNCVkeHUh?=
UM-UC-3	M1Tnc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				Mn;xTWM2OD1zMEOuPFQzKM7:TR?=	NXrCZXFSW0GQR1XS
SNU-C2B	NWmydGpUT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NXHMcY1UUUN3ME2xNFUvODJ\|IN88US=>	MnPEV2FPT0WU
RPMI-8226	M3ixR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M1PzR2lEPTB;MUC2MlY4QCEQvF2=	MVjTRW5ITVJ?
PANC-08-13	M{f2Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MX\JR\|UxRTFyNj64OUDPxE1?	Mo\aV2FPT0WU
NCI-H23	NVzWPYd{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NX\ZN5F7UUN3ME2xNFgvOzV5IN88US=>	NG\OZ2xUSU6JRWK=
HOP-92	M{DBV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M4fIeGlEPTB;MUC4MlQ4PSEQvF2=	NGnkVG1USU6JRWK=
FTC-133	NHe4cmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MYDJR\|UxRTFyOD60PFgh\|ryP	Ml3MV2FPT0WU
D-263MG	MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MXnJR\|UxRTFyOD65OlEh\|ryP	NH;RTWxUSU6JRWK=
DU-145	M3SwOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NWnvflJ6UUN3ME2xNFkvQTR3IN88US=>	NU[zb3dJW0GQR1XS
A253	NVTPNJltT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M4jUTGlEPTB;MUGwMlQ4QSEQvF2=	NEnaWmtUSU6JRWK=
HCT-15	MlLES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MVTJR\|UxRTFzMD62OlEh\|ryP	NUjUOGIzW0GQR1XS
WSU-NHL	NH3heo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MVrJR\|UxRTFzMj6wNUDPxE1?	Mo\HV2FPT0WU
U031	Mo\0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				Mo[zTWM2OD1zMUKuNFY2KM7:TR?=	M1H2eXNCVkeHUh?=
MDA-MB-157	M3vsfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NUXVXHI2UUN3ME2xNVIvPDlizszN	NEnYW\|FUSU6JRWK=
NEC8	NF\uTXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1PGfmlEPTB;MUGyMlYzPCEQvF2=	MojPV2FPT0WU
SIG-M5	NEOxT29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M4\XUmlEPTB;MUGzMlEzPSEQvF2=	NVPsUnU{W0GQR1XS
786-0	MmrDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NH[0R5VKSzVyPUGxN{41PTNizszN	NGPYeGdUSU6JRWK=
DMS-273	M2DmN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				Mn30TWM2OD1zMUWuOlE4KM7:TR?=	M1nsd3NCVkeHUh?=
Calu-6	MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MknITWM2OD1zMU[uO\|k5KM7:TR?=	NWK2WZkxW0GQR1XS
HCC1599	NYTwUWh1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MV7JR\|UxRTFzNz6xOVYh\|ryP	NVn5cpp4W0GQR1XS
OVCAR-8	MmOzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M3L2TmlEPTB;MUG3MlY3QCEQvF2=	NUKwXGRmW0GQR1XS
ATN-1	MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M37xdGlEPTB;MUG3MlgzQCEQvF2=	NXHyb2hmW0GQR1XS
RKO	MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M4\EemlEPTB;MUG3Mlg6QCEQvF2=	MX;TRW5ITVJ?
SKG-IIIa	NIjRe3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MoT2TWM2OD1zMUiuNVE3KM7:TR?=	M2HiR3NCVkeHUh?=
SUP-T1	M4\qfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M3i4OmlEPTB;MUG4Mlk2PiEQvF2=	NW[yNIE{W0GQR1XS
KNS-81-FD	MoD0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MVfJR\|UxRTFzOT60NVch\|ryP	NUT0dpdVW0GQR1XS
OVCAR-3	NIXMWHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NHjEcnZKSzVyPUGxPU46OTVizszN	MlLOV2FPT0WU
NCI-N87	NYfYTFkzT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M3fmb2lEPTB;MUKxMlA1PCEQvF2=	M3rlN3NCVkeHUh?=
HuP-T4	NVfRSHZpT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MUnJR\|UxRTF{MT61OUDPxE1?	NEHzOpJUSU6JRWK=
KYSE-140	MnvUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NYjHc2dUUUN3ME2xNlIvOTRzIN88US=>	MUDTRW5ITVJ?
HAL-01	MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NX76NWhYUUN3ME2xNlIvPDl7IN88US=>	MYDTRW5ITVJ?
HT-29	M3Tue2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MWXJR\|UxRTF{Mj63O\|gh\|ryP	NW\yUXh1W0GQR1XS
OPM-2	MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MXvJR\|UxRTF{Mz6xO\|Yh\|ryP	M1LhVXNCVkeHUh?=
SW1463	MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NWjyWlBEUUN3ME2xNlQvODd5IN88US=>	NWPpPVZoW0GQR1XS
NCI-H2087	M17Hb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NGDWXndKSzVyPUGyOE4zQDNizszN	NWWzTWdHW0GQR1XS
UMC-11	MkP4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NUPjR\|VvUUN3ME2xNlYvODN6IN88US=>	M3LjTHNCVkeHUh?=
EB-3	NF;SN\|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NIjNSoNKSzVyPUGyO{4xOTNizszN	NVzSSHp2W0GQR1XS
HEC-1	MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MoCyTWM2OD1zMkmuNVk5KM7:TR?=	M1r5THNCVkeHUh?=
LU-99A	M4LUemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NInG[VhKSzVyPUGyPU46OzFizszN	M2XWbHNCVkeHUh?=
VMRC-RCZ	M3LGeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MYPJR\|UxRTF\|MD6zO\|Uh\|ryP	NUnqcXBuW0GQR1XS
MFH-ino	NYj2Npc3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M4PSS2lEPTB;MUOxMlEyPCEQvF2=	M{\ZXHNCVkeHUh?=
HT-1080	M3W0cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NUfre3hrUUN3ME2xN\|IvOjB2IN88US=>	NIjGepNUSU6JRWK=
CTB-1	M{LIUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MXHJR\|UxRTF\|Mj64PVch\|ryP	NVu3e\|NVW0GQR1XS
Capan-2	MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M2XiOWlEPTB;MUOzMlk4PSEQvF2=	MWfTRW5ITVJ?
BB30-HNC	NUPhVWRET3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NX61WppPUUN3ME2xN\|QvOTF4IN88US=>	MoW4V2FPT0WU
CP50-MEL-B	NFj2NoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				Ml7wTWM2OD1zM{SuNkDPxE1?	MkDLV2FPT0WU
JEG-3	NID4WJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MYfJR\|UxRTF\|ND6zOVkh\|ryP	NV7NZVV{W0GQR1XS
NCI-H1437	NV7yNlhsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NWnEOm95UUN3ME2xN\|QvPTlizszN	M1PhPHNCVkeHUh?=
NOS-1	NVToc3dFT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MlzkTWM2OD1zM{SuO\|Y2KM7:TR?=	Mn;KV2FPT0WU
G-402	MkPqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MnnZTWM2OD1zM{WuNlk2KM7:TR?=	NUTsVlhZW0GQR1XS
OAW-42	MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MmHxTWM2OD1zM{euPFU3KM7:TR?=	NHn4T5pUSU6JRWK=
Ca-Ski	NX;wWIYzT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NGDMTVRKSzVyPUGzPE45OzFizszN	MnrnV2FPT0WU
BHT-101	NYPIdVRJT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NYT3WoY{UUN3ME2xOFAvOjB6IN88US=>	MknIV2FPT0WU
OVCAR-4	NUDDbVluT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MkPNTWM2OD1zNECuO\|Q6KM7:TR?=	MVHTRW5ITVJ?
Detroit562	NVq5fYlVT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				Mn3ZTWM2OD1zNEGuOFY3KM7:TR?=	MnuxV2FPT0WU
OCI-AML2	NEXKR45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NU\JOpZEUUN3ME2xOFEvPTB5IN88US=>	MmLwV2FPT0WU
OE33	Mn7TS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MUfJR\|UxRTF2MT63PVgh\|ryP	NHq4Om1USU6JRWK=
YKG-1	M173TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NYHNS5JRUUN3ME2xOFIvQDh7IN88US=>	NXvyWHl1W0GQR1XS
CCF-STTG1	MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NHS2V2NKSzVyPUG0N{4{OzRizszN	NXrSSYY1W0GQR1XS
NCI-H810	MmL0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MmS4TWM2OD1zNEOuOlg4KM7:TR?=	NWrmVJc{W0GQR1XS
SCC-25	NYrYTolQT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				Mk\JTWM2OD1zNESuNFA2KM7:TR?=	MXnTRW5ITVJ?
HTC-C3	NXvNdVdVT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NGfYSJVKSzVyPUG0OU4xPzVizszN	MorUV2FPT0WU
G-401	NEL6NVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MnfLTWM2OD1zNEWuPFM2KM7:TR?=	MkP3V2FPT0WU
OMC-1	M4XhVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NVPR[FN5UUN3ME2xOFUvQTZ5IN88US=>	MXrTRW5ITVJ?
LK-2	NXriWJBmT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NVjVNWt1UUN3ME2xOFcvOTR\|IN88US=>	NWn4dmxMW0GQR1XS
SW13	NHu3[INIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MlK2TWM2OD1zNEeuNlU{KM7:TR?=	M1zPVnNCVkeHUh?=
BB49-HNC	M4jp[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NELC[FlKSzVyPUG0O{41ODRizszN	NYK3UJc6W0GQR1XS
DMS-114	M{DEbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M3y0NGlEPTB;MUS3MlQ1OSEQvF2=	NVrVNIszW0GQR1XS
GCIY	NIiwepJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MVLJR\|UxRTF2Nz63PVch\|ryP	M{HLZ3NCVkeHUh?=
GAMG	NGT3OYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MUHJR\|UxRTF2OD6yOFch\|ryP	NYfhXohMW0GQR1XS
KYSE-520	Mn3oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MnLsTWM2OD1zNEiuOVQh\|ryP	Mmi3V2FPT0WU
LB647-SCLC	NH\xOo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NITSbHdKSzVyPUG0PE42PjJizszN	M2HyTXNCVkeHUh?=
639-V	M3noSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M{L3XWlEPTB;MUS4MlcxPSEQvF2=	NELWN\|NUSU6JRWK=
TE-9	NHq5cmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1frVGlEPTB;MUS5MlQ1OyEQvF2=	NVq2V3J2W0GQR1XS
Caov-3	MmTmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MYjJR\|UxRTF2OT63NVQh\|ryP	MnHVV2FPT0WU
L-540	MlPMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NWTsWXRuUUN3ME2xOFkvPzVizszN	M1;IfnNCVkeHUh?=
NCI-H748	MnnMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MmTETWM2OD1zNUCuNlA6KM7:TR?=	NIDVZ\|BUSU6JRWK=
GDM-1	M2DvUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NGPNTmlKSzVyPUG1NU4yOzVizszN	MYTTRW5ITVJ?
GAK	MmroS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MXLJR\|UxRTF3MT6zO\|Qh\|ryP	M2m4[XNCVkeHUh?=
AsPC-1	M33DO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NGXoOm9KSzVyPUG1Nk44PjRizszN	M{e2dHNCVkeHUh?=
MDA-MB-361	NIDtcXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MonDTWM2OD1zNUOuNVY1KM7:TR?=	NX3Ye\|ZQW0GQR1XS
TT	Mnj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NH64bVZKSzVyPUG1N{41QSEQvF2=	NXvPR2prW0GQR1XS
Calu-1	MnL5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M1S4VWlEPTB;MUW0MlAxOyEQvF2=	MkPpV2FPT0WU
RERF-LC-MS	NV35UJRMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NHL6SVNKSzVyPUG1OE44PzJizszN	NULrb5RiW0GQR1XS
DK-MG	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MVjJR\|UxRTF3NT6xPFMh\|ryP	NUDvblJkW0GQR1XS
CAL-120	NVTZS5Z2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MVLJR\|UxRTF3NT64NlEh\|ryP	NWi4cYxLW0GQR1XS
HCC1419	NGi5[olIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MX3JR\|UxRTF3Nj6wO\|ch\|ryP	NFzFU3pUSU6JRWK=
DG-75	M{TWWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NFXZSWFKSzVyPUG1Ok43QTRizszN	NUjrcI5kW0GQR1XS
HCC38	MmP0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NEHpelBKSzVyPUG1O{4{PjlizszN	M3vnSnNCVkeHUh?=
HCC70	NGXIb4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NYDvTpBYUUN3ME2xOVkvOjR{IN88US=>	NFjhUVlUSU6JRWK=
ES4	NXfZSYFIT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NWGxPXdLUUN3ME2xOVkvOzl4IN88US=>	M3TMenNCVkeHUh?=
NCI-H2052	NH\nWlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NWHjb4k6UUN3ME2xOVkvPTh\|IN88US=>	NHzMXXJUSU6JRWK=
EFO-27	NIXYVnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NICwV2RKSzVyPUG2NE42QDRizszN	MV\TRW5ITVJ?
CAL-62	NELkb3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MkXvTWM2OD1zNkKuOlE{KM7:TR?=	M3Tle3NCVkeHUh?=
CHP-212	NUT6W2RsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M3PSfWlEPTB;MU[zMlYzPiEQvF2=	NXmzdWJ7W0GQR1XS
AU565	NHX4NoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M4SyTGlEPTB;MU[zMlY1KM7:TR?=	M{DtbnNCVkeHUh?=
Ca9-22	NYP4R4tST3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MWjJR\|UxRTF4ND6zNlEh\|ryP	MYjTRW5ITVJ?
EFO-21	Mlz3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NYrjRYlLUUN3ME2xOlUvPDVizszN	NGnUOllUSU6JRWK=
KINGS-1	MkPoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MY\JR\|UxRTF4Nz6zOVQh\|ryP	MmTqV2FPT0WU
JVM-2	NWP2d\|dRT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				Ml[1TWM2OD1zNkeuOFUyKM7:TR?=	NFfjNmRUSU6JRWK=
OVCAR-5	MlG5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MmPTTWM2OD1zNkiuN\|A6KM7:TR?=	MWjTRW5ITVJ?
Calu-3	MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MXjJR\|UxRTF4OD6zNVEh\|ryP	MkLTV2FPT0WU
CP66-MEL	NX\tRnFLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				Mkm3TWM2OD1zNkiuOFE2KM7:TR?=	MmTkV2FPT0WU
KYSE-150	NH;VS3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1TQe2lEPTB;MU[5MlY{PiEQvF2=	NIDDU3lUSU6JRWK=
Daoy	MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NGq2PIxKSzVyPUG2PU45QTFizszN	M4\qT3NCVkeHUh?=
HUTU-80	NW\sSXp2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NXXQemNnUUN3ME2xO\|EvODN{IN88US=>	M3e5eXNCVkeHUh?=
SW620	M3z6cWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M174WGlEPTB;MUexMlU{PSEQvF2=	Ml3pV2FPT0WU
HuO-3N1	Mk\lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M4rmfGlEPTB;MUeyMlI4OyEQvF2=	NUnFcZpGW0GQR1XS
HH	NILnRVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NHfPV25KSzVyPUG3N{45PDhizszN	MWjTRW5ITVJ?
ME-180	MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MXPJR\|UxRTF5ND6wNVYh\|ryP	NInK[pFUSU6JRWK=
SW756	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NEPieGtKSzVyPUG3OE45PjNizszN	Ml7jV2FPT0WU
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NCI-H522	MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MYHJR\|UxRTF6MT6wOVkh\|ryP	M1;JNnNCVkeHUh?=
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DOK	NX7HcWhQT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NIDINodKSzVyPUG4OE43PDNizszN	M1vBeXNCVkeHUh?=
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LB2241-RCC	MnLQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MkXiTWM2OD1zOEWuNVQ2KM7:TR?=	Mo\xV2FPT0WU
NCI-H1563	M1rsZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MoLWTWM2OD1zOEWuNlA6KM7:TR?=	M3PtfHNCVkeHUh?=
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MONO-MAC-6	MmPrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MoLyTWM2OD1zOEeuN\|kzKM7:TR?=	M3HCSnNCVkeHUh?=
CAPAN-1	M1HHb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NF\CUVRKSzVyPUG4O{41PTNizszN	MnvvV2FPT0WU
SK-LU-1	NFPpT4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M3P0RmlEPTB;MUi4MlQzQCEQvF2=	M2PCU3NCVkeHUh?=
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NCI-H838	MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NUXkTFd3UUN3ME2xPVAvOzRizszN	NV;l[GVsW0GQR1XS
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COLO-679	MkL5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NIX3coFKSzVyPUKwNE45PTlizszN	M{LUTnNCVkeHUh?=
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NCI-H345	MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NXO1OpFLUUN3ME2yNFMvODJ5IN88US=>	NYjQNIhzW0GQR1XS
KYSE-70	NHPyR4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				Moe1TWM2OD1{MEOuPFc6KM7:TR?=	MonhV2FPT0WU
EGI-1	NUW4OHZ4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MVjJR\|UxRTJyND64N\|Mh\|ryP	MYPTRW5ITVJ?
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NCI-H1651	M1PDTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MUPJR\|UxRTJyNj63N{DPxE1?	MVrTRW5ITVJ?
HPAF-II	M3LpRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M1foZ2lEPTB;MkC5MlY4OSEQvF2=	MXfTRW5ITVJ?
NUGC-3	M3\vT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MnnoTWM2OD1{MEmuOlc5KM7:TR?=	M3PJWXNCVkeHUh?=
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MHH-PREB-1	MnjoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NHnIe\|VKSzVyPUKxNE4{QDFizszN	NUPTNG1FW0GQR1XS
EW-18	NYDyZ2FMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MUTJR\|UxRTJzMD63OlMh\|ryP	NV\ESJVpW0GQR1XS
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HO-1-N-1	NF7kN5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				Moq5TWM2OD1{MUKuPFk1KM7:TR?=	NYPaRmY2W0GQR1XS
NCI-H69	Mk\WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M2\4W2lEPTB;MkG0MlE5PSEQvF2=	NV6xZmVGW0GQR1XS
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NCI-H1618	NV:zc4M{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NHjQTVJKSzVyPUKyNU4xOThizszN	MlnJV2FPT0WU
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NCI-H1355	NITxVo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MVXJR\|UxRTJ{MT6zO\|ch\|ryP	M1XrWXNCVkeHUh?=
NCI-H1993	MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NUDQ[2NHUUN3ME2yNlIvOjN7IN88US=>	NUeyc5ZIW0GQR1XS
NCI-H1623	MlLhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NXi0cXI3UUN3ME2yNlIvOzh7IN88US=>	MUDTRW5ITVJ?
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HCC1937	M4rufmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NWHtSlR7UUN3ME2yNlQvOzh7IN88US=>	NXvidXFVW0GQR1XS
NCI-H209	Moi4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MUHJR\|UxRTJ{Nj61N{DPxE1?	MV7TRW5ITVJ?
NCI-H322M	M1\jNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NELPe2JKSzVyPUKyPE4xOTlizszN	MlTrV2FPT0WU
CHL-1	M{WxfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MofITWM2OD1{MkiuNVMh\|ryP	MlroV2FPT0WU
NCI-H630	Ml3wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MmrLTWM2OD1{M{KuPFk4KM7:TR?=	M4\POnNCVkeHUh?=
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NCI-H520	NF7hbFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NUDIS21OUUN3ME2yN\|UvPzRzIN88US=>	M2GzTHNCVkeHUh?=
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SK-HEP-1	NYLTOYc4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MV7JR\|UxRTJ2Nj6wOFMh\|ryP	NGT5O\|FUSU6JRWK=
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KYSE-510	MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M3;H[GlEPTB;MkS3MlUyPyEQvF2=	NEHIXY5USU6JRWK=
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NCI-H1693	MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NUTr[5FUUUN3ME2yOFkvPDl3IN88US=>	NHqxbJZUSU6JRWK=
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NCI-H2228	MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MU\JR\|UxRTJ3MD64OlQh\|ryP	MnHCV2FPT0WU
NCI-H460	MljFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NXHXcGtTUUN3ME2yOVEvPDF5IN88US=>	M1jYc3NCVkeHUh?=
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CAL-27	NIW5bmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1[4bGlEPTB;MkWzMlQ5PSEQvF2=	MorNV2FPT0WU
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NCI-H720	M3\NeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				Ml;4TWM2OD1\|NEmuPFQ3KM7:TR?=	NWnNeWF[W0GQR1XS
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COLO-684	MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NVzm[W1lUUN3ME20OFgvQDh6IN88US=>	M4m1NXNCVkeHUh?=
DMS-79	NGHj[o9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M{f3fWlEPTB;NES5MlY5KM7:TR?=	NV7sOJl7W0GQR1XS
EW-1	NVfJXYxET3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MWHJR\|UxRTR3Mj64PFgh\|ryP	M1TEOXNCVkeHUh?=
MS-1	M3HEfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MVrJR\|UxRTR3ND6zPFUh\|ryP	MWDTRW5ITVJ?
A4-Fuk	MmfVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MkPkTWM2OD12NUSuOVA4KM7:TR?=	M3e1enNCVkeHUh?=
TALL-1	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NVTXbHB7UUN3ME20OVUvOjl5IN88US=>	M4nUZXNCVkeHUh?=
SCLC-21H	NUK5SFExT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NX3nTlRuUUN3ME20OVUvPjl7IN88US=>	NGnrZoNUSU6JRWK=
KMS-12-PE	NXfNdJVJT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NW\lXYlPUUN3ME20OVYvQDJ{IN88US=>	NILONVBUSU6JRWK=
TE-441-T	MmK0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NXfLRm5vUUN3ME20OVcvOTh4IN88US=>	MlrpV2FPT0WU
A3-KAW	Ml;sS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NGnvclVKSzVyPUS2NE45PDFizszN	MVrTRW5ITVJ?
NCI-H1092	NVrYbZhLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M4TEbmlEPTB;NE[zMlk1OyEQvF2=	NHrNXVNUSU6JRWK=
COR-L279	M2Kwemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MnnkTWM2OD12NkSuPFQ4KM7:TR?=	M4r6VXNCVkeHUh?=
NCI-SNU-5	NUDtRYRFT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NVnsN4VkUUN3ME20O\|IvOjhizszN	MX\TRW5ITVJ?
JAR	MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NXy4cFRGUUN3ME20O\|QvPzh6IN88US=>	NWS4OYo4W0GQR1XS
NCI-H446	M4\pS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MlPmTWM2OD12N{euNFgh\|ryP	NX;yS3FGW0GQR1XS
HDLM-2	NWXCe2NST3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M1u2XGlEPTB;NEiwMlg3QSEQvF2=	Mn;mV2FPT0WU
D-283MED	MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MV7JR\|UxRTR6OD65NlQh\|ryP	NV:4[JB5W0GQR1XS
NCI-H1395	NHn1d4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MWPJR\|UxRTR6OT6wNFQh\|ryP	NI\2TmtUSU6JRWK=
RPMI-6666	MnnpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M3;XemlEPTB;NEmxMlM6QCEQvF2=	NIO4SYZUSU6JRWK=
CPC-N	MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M3K5Z2lEPTB;NUCyMlA3OyEQvF2=	MYjTRW5ITVJ?
EC-GI-10	NV3RTZg{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M1nNRWlEPTB;NUCyMlE3QSEQvF2=	MUPTRW5ITVJ?
CA46	MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NVvoT3hOUUN3ME21NFQvPDB5IN88US=>	Mk[zV2FPT0WU
EHEB	NF:1VFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M4Pjc2lEPTB;NUC3MlIxQCEQvF2=	Mnz4V2FPT0WU
RPMI-8402	MmfLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NV\hVFdQUUN3ME21NVAvODJzIN88US=>	Mm\DV2FPT0WU
DU-4475	Mmq3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NGD3SHFKSzVyPUWxNU4yODFizszN	MYPTRW5ITVJ?
P12-ICHIKAWA	NIHrTmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MVTJR\|UxRTVzMT60NVYh\|ryP	NE[0RYlUSU6JRWK=
NCI-H1694	MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MmDPTWM2OD13MUKuOFM6KM7:TR?=	MorJV2FPT0WU
NCI-H2171	NHvJOWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MkfOTWM2OD13MUKuO\|A2KM7:TR?=	NHXDcZRUSU6JRWK=
Mo-T	MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M{XMcGlEPTB;NUG1MlEyPiEQvF2=	NU\rNnc2W0GQR1XS
HL-60	MoTSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MVzJR\|UxRTV{Mj65NlQh\|ryP	NEDxTJBUSU6JRWK=
NCI-H1436	NGrlN5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NHWzU5pKSzVyPUWyN{4xPjRizszN	MmiyV2FPT0WU
NCI-H510A	NYj4OWhNT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MlTyTWM2OD13MkSuOlk3KM7:TR?=	MWHTRW5ITVJ?
NCI-H2081	NYPC[ow4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NIPtcmtKSzVyPUWyOU42OTNizszN	NXXGemR[W0GQR1XS
NB5	NFTzZoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NYLhNpF4UUN3ME21NlYvPDNizszN	MXPTRW5ITVJ?
NCI-H2227	NXfudnpOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MlzqTWM2OD13M{CuNlU1KM7:TR?=	NGD4[npUSU6JRWK=
HSC-4	MkGzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M{fxcGlEPTB;NUO5MlI6OyEQvF2=	MlHlV2FPT0WU
RL	MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NVTPO4RPUUN3ME21OFIvPDR{IN88US=>	MlPuV2FPT0WU
EVSA-T	NVjIcpI3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M4TrXGlEPTB;NUSzMlY5PCEQvF2=	NWfPbGxuW0GQR1XS
NCI-H889	NFLyV\|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MnO0TWM2OD13NEeuNlMh\|ryP	MYTTRW5ITVJ?
COLO-320-HSR	MofUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NUnicIlMUUN3ME21OVEvOzVizszN	NIX0[G5USU6JRWK=
EW-11	M{PkVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M1TH[mlEPTB;NUWyMlM6QSEQvF2=	MWHTRW5ITVJ?
UACC-812	MmHtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NFe1Om9KSzVyPUW1PU4{QTFizszN	NUjSVWFMW0GQR1XS
CAL-148	NG\aNGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MV3JR\|UxRTV4NT6wPFkh\|ryP	NYHR[oY6W0GQR1XS
K052	MmrSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MkizTWM2OD13N{muNlE{KM7:TR?=	MXXTRW5ITVJ?
L-428	MkPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NVvEb5k5UUN3ME22NFAvPjdzIN88US=>	Mk\zV2FPT0WU
LP-1	M{LZ[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M2rUcWlEPTB;NkCzMlU5PyEQvF2=	MXHTRW5ITVJ?
ML-2	NFTR[o1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MUfJR\|UxRTZyNj61O\|kh\|ryP	NGjTVYZUSU6JRWK=
KARPAS-45	NVzTcIFYT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M4n1W2lEPTB;NkG4MlIzOSEQvF2=	NET4d\|lUSU6JRWK=
HT	M4rWWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M3Hhb2lEPTB;NkKyMlc5PSEQvF2=	M{TQTnNCVkeHUh?=
NCI-H226	NVnPbFZXT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NGC0WHFKSzVyPU[yPE4{PTNizszN	NGHCTJlUSU6JRWK=
L-363	MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M3LtSGlEPTB;NkS4MlA3PCEQvF2=	MWTTRW5ITVJ?
IM-9	NFLtSVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1PWTmlEPTB;NkWxMlU1OSEQvF2=	M{fydHNCVkeHUh?=
TUR	M2jhO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				Ml\1TWM2OD14N{GuN\|IzKM7:TR?=	NFfUcoxUSU6JRWK=
NCI-SNU-16	M{L3c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M4S1d2lEPTB;NkiwMlQ3OiEQvF2=	NHzoTFdUSU6JRWK=
NCI-SNU-1	MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MYPJR\|UxRTZ7MD6xO\|Ih\|ryP	NYfxVIFLW0GQR1XS
U-87-MG	MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NGDyWZNKSzVyPU[5OE42QDVizszN	MYjTRW5ITVJ?
KM-H2	MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MkTyTWM2OD14OUmuNFU6KM7:TR?=	NVfkNXhDW0GQR1XS
DEL	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NVz5VGR7UUN3ME23NFAvQDN6IN88US=>	M{HIeXNCVkeHUh?=
KARPAS-422	NWi2SW1FT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NF[xd3hKSzVyPUe4PU4{OTVizszN	NEPucFJUSU6JRWK=
BC-3	MoHPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NF[xWIdKSzVyPUiwOU4zODNizszN	MkL0V2FPT0WU
NCI-H1155	M3jmOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MlPqTWM2OD16MUiuNFU6KM7:TR?=	NGnjd3FUSU6JRWK=
NCI-H1581	NGX3[VVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NYmxZ4NIUUN3ME25NFAvOjNzIN88US=>	NWLxfGJEW0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo

Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. ^[1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. ^[4]

Protocol

Kinase Assay: ^[1]	+ Expand Cell-free kinase assays: Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl₂, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research: ^[3]	+ Expand Cell lines: INS-1E Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM Incubation Time: 1, or 4 days Method: Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system. (Only for Reference)
Animal Research: ^[1]	+ Expand Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes Formulation: Formulated as solutions in 20 mM citrate-buffered 15% Captisol or as fine suspensions in 0.5% carboxymethylcellulose. Dosages: ~48 mg/kg Administration: Orally (Only for Reference)

References

Solubility (25°C)

In vitro	DMSO	93 mg/mL (185.33 mM)
	Ethanol	2 mg/mL (3.98 mM)
	Water	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 5% DMSO+30% PEG 300+ddH2O For best results, use promptly after mixing.	24mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download CHIR-99021 (CT99021) HCl SDF

Molecular Weight	501.8
Formula	C₂₂H₁₈Cl₂N_8.HCl
CAS No.	1797989-42-4
Storage	3 years -20°C powder
Storage	2 years -80°C in solvent
Synonyms	N/A

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?
Answer:
The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.
Question 2:
How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?
Answer:
Please change the culture medium each day to add the new comound in it if the duration is more than 72h.
Question 3:
What is the difference between S2924 and S1263? Which one is better for differentiation?
Answer:
S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

GSK-3 Signaling Pathway Map

GSK-3 Inhibitors with Unique Features

Selective GSK-3 Inhibitor

TWS119 : GSK-3β-selective, IC50 of 30 nM.
Most Potent GSK-3 Inhibitor

CHIR-98014 : GSK-3α, IC50=0.65 nM; GSK-3β, IC50=0.58 nM.
GSK-3 Inhibitor in Clinical Trial

Tideglusib : Phase II for Alzheimer's disease.
Newest GSK-3 Inhibitor

AZD1080 : Orally active, brain permeable GSK3 inhibitor, inhibits human GSK3α and GSK3β with K_i of 6.9 nM and 31 nM, respectively.

Related GSK-3 Products4

CHIR-99021 (CT99021) ^New

CHIR-99021 (CT99021) is a GSK-3α and GSK-3β inhibitor with IC50 of 10 nM and 6.7 nM, respectively. CHIR99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) and shows a 350-fold selectivity toward GSK-3β compared to CDKs.
LY2090314 ^New

LY2090314 is a potent GSK-3 inhibitor for GSK-3α/β with IC50 of 1.5 nM/0.9 nM; may improve the efficacy of platinum-based chemotherapy regimens. LY2090314 is highly selective towards GSK3 as demonstrated by its fold selectivity relative to a large panel of kinases.
TDZD-8 ^New

TDZD-8 is a non-ATP competitive GSK-3β inhibitor with IC50 of 2 μM; minimal inhibitory effect observed on CDK1, casein kinase II, PKA and PKC.
TWS119

TWS119 is a GSK-3β inhibitor with IC50 of 30 nM in a cell-free assay; capable of inducing neuronal differentiation and may be useful to stem cell biology.
SB216763

SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.
Tideglusib

Tideglusib is an irreversible, non ATP-competitive GSK-3β inhibitor with IC50 of 60 nM in a cell-free assay; fails to inhibit kinases with a Cys homologous to Cys-199 located in the active site. Phase 2.
CHIR-98014

CHIR-98014 is a potent GSK-3α/β inhibitor with IC50 of 0.65 nM/0.58 nM in cell-free assays, with the ability to distinguish GSK-3 from its closest homologs Cdc2 and ERK2.
BIO

BIO is a specific inhibitor of GSK-3 with IC50 of 5 nM for GSK-3α/β in a cell-free assay, shows >16-fold selectivity over CDK5, also a pan-JAK inhibitor.

Features:The first pharmacological agent shown to maintain self-renewal in human and mouse embryonic stem cells.
AR-A014418

AR-A014418 is an ATP-competitive, and selective GSK3β inhibitor with IC50 and K_i of 104 nM and 38 nM in cell-free assays, without significant inhibition on 26 other kinases tested.
IM-12

IM-12 is a selective GSK-3β inhibitor with IC50 of 53 nM, and also enhances canonical Wnt signalling.

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CHIR-99021 (CT99021) HCl

Cited by 73 Publications

9 Customer Reviews

Purity & Quality Control

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Biological Activity

Protocol

References

Solubility (25°C)

Chemical Information Download CHIR-99021 (CT99021) HCl SDF

Bio Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Dilution Calculator

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

The Serial Dilution Calculator Equation

Serial Dilutions

Computed Result

Molecular Weight Calculator

Total Molecular Weight: g/mol

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molarity Calculator

Tech Support

Frequently Asked Questions

GSK-3 Signaling Pathway Map

GSK-3 Inhibitors with Unique Features

Related GSK-3 Products4

Choose Your Country or Region

By Signaling Pathways

By product type

CHIR-99021 (CT99021) HCl

Cited by 73 Publications

9 Customer Reviews

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Protocol

References

Solubility (25°C)

Chemical Information Download CHIR-99021 (CT99021) HCl SDF

Bio Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Dilution Calculator

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

The Serial Dilution Calculator Equation

Serial Dilutions

Computed Result

Molecular Weight Calculator

Total Molecular Weight: g/mol

Instructions to calculate molar mass (molecular weight) of a chemical compound:

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molarity Calculator

Tech Support

Frequently Asked Questions

GSK-3 Signaling Pathway Map

GSK-3 Inhibitors with Unique Features

Related GSK-3 Products4

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)