Sorafenib (BAY 43-9006)

For research use only.

Catalog No.S7397 Synonyms: NSC-724772

389 publications

Sorafenib (BAY 43-9006) Chemical Structure

CAS No. 284461-73-0

Sorafenib (BAY 43-9006, NSC-724772) is a multikinase inhibitor of Raf-1 and B-Raf with IC50 of 6 nM and 22 nM in cell-free assays, respectively. Sorafenib inhibits VEGFR-2, VEGFR-3, PDGFR-β, Flt-3 and c-KIT with IC50 of 90 nM, 20 nM, 57 nM, 59 nM and 68 nM, respectively. Sorafenib induces autophagy and apoptosis and activates ferroptosis with anti-tumor activity.

Selleck's Sorafenib (BAY 43-9006) has been cited by 389 publications

Purity & Quality Control

Choose Selective Raf Inhibitors

Biological Activity

Description Sorafenib (BAY 43-9006, NSC-724772) is a multikinase inhibitor of Raf-1 and B-Raf with IC50 of 6 nM and 22 nM in cell-free assays, respectively. Sorafenib inhibits VEGFR-2, VEGFR-3, PDGFR-β, Flt-3 and c-KIT with IC50 of 90 nM, 20 nM, 57 nM, 59 nM and 68 nM, respectively. Sorafenib induces autophagy and apoptosis and activates ferroptosis with anti-tumor activity.
Targets
Raf-1 [1]
(Cell-free assay)
mVEGFR2(Flk1) [1]
(Cell-free assay)
mVEGFR3 [1]
(Cell-free assay)
B-Raf [1]
(Cell-free assay)
B-Raf (V599E) [1]
(Cell-free assay)
6 nM 15 nM 20 nM 22 nM 38 nM
In vitro

Sorafenib inhibits both wild-type and V599E mutant B-Raf activity with IC50 of 22 nM and 38 nM, respectively. Sorafenib also potently inhibits mVEGFR2 (Flk-1), mVEGFR3, mPDGFRβ, Flt3, and c-Kit with IC50 of 15 nM, 20 nM, 57 nM, 58 nM, and 68 nM, respectively. Sorafenib weakly inhibits FGFR-1 with IC50 of 580 nM. Sorafenib tosylate is not active against ERK-1, MEK-1, EGFR, HER-2, IGFR-1, c-Met, PKB, PKA, cdk1/cyclinB, PKCα, PKCγ, and pim-1. Sorafenib markedly inhibits VEGFR2 phosphorylation in NIH 3T3 cells with IC50 of 30 nM, and Flt-3 phosphorylation in HEK-293 cells with IC50 of 20 nM. Sorafenib potently blocks MEK 1/2 and ERK 1/2 phosphorylation in most cell lines but not in A549 or H460 cells, while having no effect on inhibition of the PKB pathway. Sorafenib inhibits the proliferation of HAoSMC and MDA-MB-231 cells with IC50 of 0.28 μM and 2.6 μM, respectively. [1] In addition to inhibition of the RAF/MEK/ERK signaling pathway, Sorafenib significantly inhibits the phosphorylation of eIF4E and down-regulates Mcl-1 levels in hepatocellular carcinoma (HCC) cells in a MEK/ERK-independent manner. Sorafenib inhibits the proliferation of PLC/PRF/5 and HepG2 cells with IC50 of 6.3 μM and 4.5 μM, respectively, and leads to the significant induction of apoptosis. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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NKM-1 MnvzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjvVHVtUUN3ME2wMlA4PDF4IN88US=> NWDmW|V5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN|Nj:nQnNCVkeHUkyvZV4>
CGTH-W-1 NV;CSFBoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTBwMkWwNlIh|ryP NFLL[4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzN4Lze+V2FPT0WUPD;hQi=>
BB65-RCC NHPw[XNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTBwNEewO|Mh|ryP MlnNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVM{Pi9pPmPBUmdGWjxxYU6=
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SH-4 M4LRVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIi5[mhKSzVyPUCuOlU3OTNizszN NYXzWVZMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN|Nj:nQnNCVkeHUkyvZV4>
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LAMA-84 NInqW2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF\KZpJKSzVyPUGuN|c3PDhizszN NX;pUGZlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN|Nj:nQnNCVkeHUkyvZV4>
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MEG-01 NHnEO3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M17DWWlEPTB;MT64NFk5KM7:TR?= NGHlcmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzN4Lze+V2FPT0WUPD;hQi=>
A3-KAW Mn;tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYHJR|UxRTFwOEi0NkDPxE1? NXjlUVZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN|Nj:nQnNCVkeHUkyvZV4>
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SF539 M3rKOGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MYHHTVUxKD1iMT61PEDPxE1? MkSxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
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U937 MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NXy5fmpHT0l3MDC9JFIvPzRizszN NFfEbW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkOxPFA3Pyd-Mk[zNVgxPjd:L3G+
U937 NVe2d5hDS3m2b4TvfIlkcXS7IHHzd4F6 M{\xcWdKPTBiPTCyMlc1KM7:TR?= NEHiclY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi3PFE6Oyd-MkS4O|gyQTN:L3G+
MCF7 Mo\MS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NI\OOWVKSzVyIE2gNk44QCEQvF2= NGPvfHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUGwNlE4PSd-MkmxNFIyPzV:L3G+
MDA-MB-231 MUPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NXrTWId1UUN3MDC9JFIvQCEQvF2= NW\KfVF2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyNFI1ODNpPkK5NlAzPDB|PD;hQi=>
HepG2 NG\YNo9CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MmTvTWM2OCB;IEKuPFQh|ryP MlH5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{NEK1OVMoRjJ6MkSyOVU{RC:jPh?=
U937 M4rjXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NY\QUnpZT0l3MDC9JFIvQDVizszN NIKwbHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkCxOFc2PSd-MkKwNVQ4PTV:L3G+
A549 M3XkZmN6fG:2b4jpZ4l1gSCjc4PhfS=> NUXPdGdpUUN3MDC9JFIvQTJizszN NFzjbI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEmyO|gxOSd-Mki5Nlc5ODF:L3G+
A549 NUnOPXZuS3m2b4TvfIlkcXS7IHHzd4F6 NE\uRZFKSzVyIE2gNk46OiEQvF2= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd5N{CwPUc,Ojd5N{ewNFk9N2F-
ZR75-30 NGrRUHpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWfNdVBsUUN3MDC9JFIvQTZizszN M1PvOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUCyNVc2Lz5{OUGwNlE4PTxxYU6=
PC3 NETGbHpEgXSxdH;4bYNqfHliYYPzZZk> M{nleWlEPTBiPTCzMlA{KM7:TR?= NF\vXG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEO0NFkyOyd-MkizOFA6OTN:L3G+
MDA-MB-231 MoDQRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NEeyd5lKSzVyIE2gN{4xQCEQvF2= M1TKdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUmxPVM5Lz5{Nkm5NVk{QDxxYU6=
A549 MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mmf1TWM2OCB;IEOuNUDPxE1? NXr6V5RERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxNFIyPzVpPkK5NVAzOTd3PD;hQi=>
K562 MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUHHTVUxKD1iMz6xOkDPxE1? MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
MOLT4 MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUjHTVUxKD1iMz6xOkDPxE1? NF7BXoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
A549/ATCC MmT6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkX1S2k2OCB;IEOuNVYh|ryP NITEelE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
RPMI8226 Mm\vS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXTUS2khRSB|LkG2JO69VQ>? NEC5VFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
SR M1ntXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH35dGNIUTVyIE2gN{4yPiEQvF2= M33rfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
HOP62 M4rZdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWfa[nZXXEeLIE2gN{4yPiEQvF2= M2L4elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
NCI-H226 MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVnrNm9mXEeLIE2gN{4yPiEQvF2= MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
U251 NIPyW4VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUXUS2khRSB|LkG2JO69VQ>? NH;IfYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
COLO205 M4P5O2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mni3WGdKKD1iMz6xOkDPxE1? NGjFUpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
M14 MYDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MorWWGdKKD1iMz6xOkDPxE1? M4PJOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
HCC2998 MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGOzeXhIUTVyIE2gN{4yPiEQvF2= NUnDT|hlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
SNB75 MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWLHTVUxKD1iMz6xOkDPxE1? NYn3UWFIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
786-0 NULJWoxsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MX7HTVUxKD1iMz6xOkDPxE1? NULlW3ZrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
A498 MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1y0b3RIUSB;IEOuNVYh|ryP MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
HCT15 MnXVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHLKRmxVT0liPTCzMlE3KM7:TR?= NFT3UIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
OVCAR3 M1\TUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml\pS2k2OCB;IEOuNVYh|ryP NHO3RZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
OVCAR4 MnHOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NULDUYpuT0l3MDC9JFMvOTZizszN NHzueHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
Caki1 Ml7NS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4LkbWdKPTBiPTCzMlE3KM7:TR?= NH7oSJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
SNB19 MlLVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1vwTWdKPTBiPTCzMlE3KM7:TR?= NHXNSYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
OVCAR5 MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmHOS2k2OCB;IEOuNVYh|ryP MnqzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
DU145 M1jwfmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIPxbZdIUTVyIE2gN{4yPiEQvF2= MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
OVCAR8 NUezR2V3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHm3[3dIUTVyIE2gN{4yPiEQvF2= NF;oe409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
SN12C NFvtbmNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX\US2khRSB|LkG2JO69VQ>? M4rvVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
MDA-MB-231 NFXiT2VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFraNVRVT0liPTCzMlE3KM7:TR?= M2r4cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
RXF393 MlrNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXHHTVUxKD1iMz6xOkDPxE1? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
BT549 MlnRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVf2[FZHT0l3MDC9JFMvOTZizszN MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
K562 NFTs[5JEgXSxdH;4bYNqfHliYYPzZZk> M2DlO2dKPTBiPTCzMlE3KM7:TR?= NY\6Tmg3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
MOLT4 NX;PUW5IS3m2b4TvfIlkcXS7IHHzd4F6 M1HXcGdKPTBiPTCzMlE3KM7:TR?= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
SNB19 M3T5fGN6fG:2b4jpZ4l1gSCjc4PhfS=> MVTHTVUxKD1iMz6xOkDPxE1? NYHHVpIzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
SR MXHDfZRwfG:6aXPpeJkh[XO|YYm= NY\ZZlZWT0l3MDC9JFMvOTZizszN MlPwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
A549 NUjDdmdWS3m2b4TvfIlkcXS7IHHzd4F6 MnG4S2k2OCB;IEOuNVYh|ryP MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
OVCAR3 MkfER5l1d3SxeHnjbZR6KGG|c3H5 NW\ZVpZMT0l3MDC9JFMvOTZizszN NXHueGpRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
OVCAR4 MWrDfZRwfG:6aXPpeJkh[XO|YYm= MX3HTVUxKD1iMz6xOkDPxE1? M{DqVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmwOVA5Lz5{NkW5NFUxQDxxYU6=
SNB75 NEXiUolEgXSxdH;4bYNqfHliYYPzZZk> NIK1eVhIUTVyIE2gN{4yPiEQvF2= M4T2RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmwOVA5Lz5{NkW5NFUxQDxxYU6=
OVCAR5 MoLWR5l1d3SxeHnjbZR6KGG|c3H5 M2G0OmdKPTBiPTCzMlE3KM7:TR?= NV3QPYF4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
DU145 MoDjR5l1d3SxeHnjbZR6KGG|c3H5 M3flZmdKPTBiPTCzMlE3KM7:TR?= NEfhZYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
786-0 MnXkR5l1d3SxeHnjbZR6KGG|c3H5 M{DRRmdKPTBiPTCzMlE3KM7:TR?= NF3qUZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
BT549 MXHDfZRwfG:6aXPpeJkh[XO|YYm= NX\CfY5ST0l3MDC9JFMvOTZizszN M4jLcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmwOVA5Lz5{NkW5NFUxQDxxYU6=
ACHN MoPqR5l1d3SxeHnjbZR6KGG|c3H5 M3nCXGdKPTBiPTCzMlE3KM7:TR?= NWGxdHhtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
Caki1 MVLDfZRwfG:6aXPpeJkh[XO|YYm= NFzqcllIUTVyIE2gN{4yPiEQvF2= MmraQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
786-0 NELVcG9CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NIH3UVlIUTVyIE2gN{4yPiEQvF2= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
LOXIMVI M3\YdWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NX;QeJl2XEeLIE2gN{4yPiEQvF2= NW[wb3BWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
Caki1 NXLmTIozSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MoPlS2k2OCB;IEOuNVYh|ryP M{DqelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
OVCAR5 M3T5S2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NUXP[pdYT0l3MDC9JFMvOTZizszN MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
RXF393 NHW3W|JCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MoXwS2k2OCB;IEOuNVYh|ryP NH\pbm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
SNB75 MoLlRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M1W1bGdKPTBiPTCzMlE3KM7:TR?= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
OVCAR3 MUTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? Ml2wS2k2OCB;IEOuNVYh|ryP MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
SNB19 M{DQeGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M{TPeGdKPTBiPTCzMlE3KM7:TR?= M13o[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
SK-MEL-5 M3rNSmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MY\US2khRSB|LkG2JO69VQ>? NFrMVXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
OVCAR4 M3vpZ2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MVPHTVUxKD1iMz6xOkDPxE1? MmHYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
SK-MEL-2 MkHpRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M3K4T3RIUSB;IEOuNVYh|ryP NEXSfpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
BT549 NHnkR5VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NYTvZYFDT0l3MDC9JFMvOTZizszN MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
MOLT4 NUOzV4pTSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M3;ZeGdKPTBiPTCzMlE3KM7:TR?= M4WxWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
K562 M{PoRWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NW\hTlQ3T0l3MDC9JFMvOTZizszN NHL0[o09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
HCC2998 M2jQSWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MknnS2k2OCB;IEOuNVYh|ryP M2G0bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
HCC2998 NIWyfYZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MlzyWGdKKD1iMz6xOkDPxE1? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
A549/ATCC NV\5[5JlSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NVXRSXhET0l3MDC9JFMvOTZizszN NF7vdHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
DU145 M4jGSGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MnGwS2k2OCB;IEOuNVYh|ryP MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
SR NGHzV5NCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NFH3[IJIUTVyIE2gN{4yPiEQvF2= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
MCF7 MnSwR5l1d3SxeHnjbZR6KGG|c3H5 NUDHbldqUUN3MDC9JFMvOThizszN MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl{N{iwNUc,Ojh7Mke4NFE9N2F-
PC3 MnTKR5l1d3SxeHnjbZR6KGG|c3H5 MnrpTWM2OCB;IEOuNVgh|ryP NXrEWVhDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5Nlc5ODFpPkK4PVI4QDBzPD;hQi=>
MCF7 MWPDfZRwfG:6aXPpeJkh[XO|YYm= M37TR2lEPTBiPTCzMlE5KM7:TR?= M1K2b|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{e3NFA6Lz5{N{e3O|AxQTxxYU6=
A549 MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NWDwW4hFUUN3MDC9JFMvOTlizszN NEHLSpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe3PFk6PSd-MkW3O|g6QTV:L3G+
PC3 MkjZR5l1d3SxeHnjbZR6KGG|c3H5 MmPkTWM2OCB;IEOuNlQh|ryP MkDQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5N{ewNFkoRjJ5N{e3NFA6RC:jPh?=
HeLa NHy3TJdHfW6ldHnvckBie3OjeR?= NHOxfGlKSzVyIE2gN{4{KM7:TR?= NF\RWYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUKyOVcxPid-MUWyNlU4ODZ:L3G+
HeLa MljESpVv[3Srb36gZZN{[Xl? NYXtepE4UUN3MDC9JFMvOyEQvF2= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTJ{NUewOkc,OTV{MkW3NFY9N2F-
LoVo MXTDfZRwfG:6aXPpeJkh[XO|YYm= NI\xfHdKSzVyIE2gN{4{KM7:TR?= MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR3NECxO{c,OjN2NUSwNVc9N2F-
A375 MmXxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NFLDe3VKSzVyIE2gN{4{PiEQvF2= NXLKdWZIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2NFI3PzRpPkK5OlAzPjd2PD;hQi=>
HT-29 MlLLRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M1;6bWlEPTBiPTCzMlM4KM7:TR?= NWSzVYQ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5PVE6OzhpPkK2PVkyQTN6PD;hQi=>
FLT3 gene-deficient U937 NIf1[ZhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M1u5WGdKPTBiPTCzMlQh|ryP MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdyOES2PEc,OjF5MEi0Olg9N2F-
FLT3 negative U937 NF;2bFREgXSxdH;4bYNqfHliYYPzZZk> NWLBZZRET0l3MDC9JFMvPCEQvF2= M3LYSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkG4O|A6Lz5{M{[xPFcxQTxxYU6=
U937 M1rHU2N6fG:2b4jpZ4l1gSCjc4PhfS=> NV;oc25WT0l3MDC9JFMvPCEQvF2= M{PJZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEixNFI{Lz5{NkC4NVAzOzxxYU6=
A375P NUjNOWV4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NHXFVJNIUTVyIE2gN{41KM7:TR?= MmL1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2NUmxOFQoRjJ7NEW5NVQ1RC:jPh?=
ACHN NGDJbpREgXSxdH;4bYNqfHliYYPzZZk> MUfJR|UxKD1iMz60JO69VQ>? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTVzN{mwPEc,Ojl3MUe5NFg9N2F-
ACHN MmHkR5l1d3SxeHnjbZR6KGG|c3H5 M33m[GlEPTBiPTCzMlQh|ryP M3zVNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7Mkm3Olg5Lz5{OUK5O|Y5QDxxYU6=
HepG2 Mm\NSpVv[3Srb36gZZN{[Xl? NGe3dmdKSzVyIE2gN{41KM7:TR?= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB5MUi2NUc,OjZyN{G4OlE9N2F-
A375P MULDfZRwfG:6aXPpeJkh[XO|YYm= MlLJS2k2OCB;IEOuOEDPxE1? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh5OEG5N{c,OjR6N{ixPVM9N2F-
HepG2 NGfpUphEgXSxdH;4bYNqfHliYYPzZZk> Ml\tTWM2OCB;IEOuOFQh|ryP NUfSXYFmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5Nlc5ODFpPkK4PVI4QDBzPD;hQi=>
HepG2 MnO4R5l1d3SxeHnjbZR6KGG|c3H5 M3vVdmlEPTBiPTCzMlQ1KM7:TR?= NWroToFFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke3O|cxODlpPkK3O|c4ODB7PD;hQi=>
ACHN MXLDfZRwfG:6aXPpeJkh[XO|YYm= MVLJR|UxKD1iMz61JO69VQ>? M2G4NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{i2OFM3Lz5{OUe4OlQ{PjxxYU6=
HepG2 NV3RN4RySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NFvuTmtKSzVyIE2gN{42KM7:TR?= M1:1dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NE[yNlY2Lz5{NUS2NlI3PTxxYU6=
HT-29 NELnVXREgXSxdH;4bYNqfHliYYPzZZk> M{XDWmlEPTBiPTCzMlYyKM7:TR?= MnjOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6Mk[4NVUoRjJ2OEK2PFE2RC:jPh?=
HT-29 NVjZR4tMSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M4rHOmlEPTBiPTCzMlYyKM7:TR?= NFz6VmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEKxOlg1QSd-M{CyNVY5PDl:L3G+
HT-29 M{PZbGN6fG:2b4jpZ4l1gSCjc4PhfS=> M4XDemlEPTBiPTCzMlYyKM7:TR?= NGX2ZYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC4OlI{QCd-MkWwPFYzOzh:L3G+
HT-29 NInGWW1EgXSxdH;4bYNqfHliYYPzZZk> M4LzbWlEPTBiPTCzMlYyKM7:TR?= NH\tbY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS0NFg4QSd-MkW0OFA5Pzl:L3G+
MCF7 MlvvRY51cWOjbnPldkBi[3Srdnn0fUBie3OjeR?= Mnj5TWM2OCB;IEOuOlQh|ryP NHS5eno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0PVg1OSd-Mkm1OFk5PDF:L3G+
Sf9 M{\MVGZ2dmO2aX;uJIF{e2G7 MlPGTWM2OCB;IEOuPEDPxE1? NHfieFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUewPFQ3QCd-MkG3NFg1Pjh:L3G+
Sf9 M1PLPGZ2dmO2aX;uJIF{e2G7 NYHYZpFYUUN3MDC9JFMvQCEQvF2= NHPlfYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[xPFcxQSd-MkO2NVg4ODl:L3G+
Sf9 M37nSGZ2dmO2aX;uJIF{e2G7 Ml\ETWM2OCB;IEOuPEDPxE1? MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MUCyN{c,OjZyOEGwNlM9N2F-
A2058 NFrHZmdEgXSxdH;4bYNqfHliYYPzZZk> NHT1[3pKSzVyIE2gN{45KM7:TR?= NVf3WWZGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3NFg6QDdpPkKyO|A5QTh5PD;hQi=>
NCI-H460 M3G5OWN6fG:2b4jpZ4l1gSCjc4PhfS=> MkHLTWM2OCB;IEOuPUDPxE1? MmHBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NUSwNVcoRjJ|NEW0NFE4RC:jPh?=
SK-MEL-30 M{Lhe2N6fG:2b4jpZ4l1gSCjc4PhfS=> MVvJR|UxKD1iMz65JO69VQ>? NHXXVm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUS2NVgzPyd-Mkm0OlE5Ojd:L3G+
HT-29 M3TSNGFvfGmlYX7j[ZIh[WO2aY\peJkh[XO|YYm= MW\JR|UxKD1iMz65O{DPxE1? M33lfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUS5PFQyLz5{OUW0PVg1OTxxYU6=
SF268 M{nm[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXfIU4pGXEeLIE2gN{46QCEQvF2= Mmr3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
SNB75 MmPIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEG5UZRVT0liPTCzMlk5KM7:TR?= M{LlXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
OVCAR3 NHzJ[mNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmjpWGdKKD1iMz65PEDPxE1? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
SNB19 NHLJdHlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUjUS2khRSB|Lkm4JO69VQ>? MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
Hs 578T NGjvVJhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGq4OFBVT0liPTCzMlk5KM7:TR?= M3PlXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
TK10 NF22XI5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn6yS2k2OCB;IEOuPVgh|ryP NUTNVopDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
BT549 M{Ppfmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYfUS2khRSB|Lkm4JO69VQ>? MmnCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
Caki1 NGnPVGxCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M2DWOHRIUSB;IEOuPVgh|ryP M4m4VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
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HCT116 Ml;YR5l1d3SxeHnjbZR6KGG|c3H5 MlXyTWM2OCB;IEWuOlUh|ryP NUfPVJRYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNVU5OThpPkK0NlE2QDF6PD;hQi=>
SH-SY5Y M4f6Z2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NX3NOXJrUUN3MDC9JFUvPzNizszN M3[2T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{G1NVkzLz5{NEOxOVE6OjxxYU6=
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HCT116 MkHYSpVv[3Srb36gZZN{[Xl? MmLpS2k2OCB;IE[uOkDPxE1? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTJ{NUewOkc,OTV{MkW3NFY9N2F-
HepG2 NUjRO2ptS3m2b4TvfIlkcXS7IHHzd4F6 MUfJR|UxKD1iNj63JO69VQ>? MkK3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzNUm0PFMoRjJ4MUW5OFg{RC:jPh?=
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A549 NXq1[nNbS3m2b4TvfIlkcXS7IHHzd4F6 MofjTWM2OCB;IE[uO{DPxE1? Ml\lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6NkWyO|YoRjJ6OE[1Nlc3RC:jPh?=
PC3 NX;veVl3S3m2b4TvfIlkcXS7IHHzd4F6 M1\rdmlEPTBiPTC2Mlgh|ryP NHvOeJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CyNVk3Pyd-MkOwNlE6Pjd:L3G+
SGC7901 NFXNNGdEgXSxdH;4bYNqfHliYYPzZZk> NGSyfoFKSzVyIE2gOk46KM7:TR?= NFX5R4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1OFAyPyd-MkO0OVQxOTd:L3G+
OS-RC2 NE\WVmFEgXSxdH;4bYNqfHliYYPzZZk> NVz3fVZwUUN3MDC9JFch|ryP NYjXSIN2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1NVc6ODhpPkK5OVE4QTB6PD;hQi=>
OS-RC2 MWLDfZRwfG:6aXPpeJkh[XO|YYm= M{TzNGlEPTBiPTC3JO69VQ>? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTJ7N{[4PEc,Ojl{OUe2PFg9N2F-
COLO205 MV;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDoeY1idg>? M3TkVmlEPTBiPTC3MlA1KM7:TR?= NY\yPFdzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyOFI2PTNpPkK4NlQzPTV|PD;hQi=>
rhabdomyosarcoma NXfFcGlDS3m2b4TvfIlkcXS7IHHjeIl3cXS7IHHnZYlve3RiaIXtZY4> MoSxR2M2OCB;IEeuNFUh|ryP MnPLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd{OEixPFYoRjJ5Mki4NVg3RC:jPh?=
HuH7 MV7DfZRwfG:6aXPpeJkh[XO|YYm= MmHUTWM2OCB;IEeuNUDPxE1? M{Ll[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUW5OFg{Lz5{NkG1PVQ5OzxxYU6=
MDA-MB-231 NVHwT4ZVSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NYrCUWlNUUN3MDC9JFcvOThizszN NEGxc5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkeyNVkzPCd-MkK3NlE6OjR:L3G+
FLT3-negative K562 MkTRR5l1d3SxeHnjbZR6KGG|c3H5 NFfTfopIUTVyIE2gO{4{KM7:TR?= NUTVcol3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NVg4ODlpPkKzOlE5PzB7PD;hQi=>
K562 M4nmc2Z2dmO2aX;uJIF{e2G7 NIjESZRIUTVyIE2gO{4{KM7:TR?= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MUCyN{c,OjZyOEGwNlM9N2F-
K562 NHPpVWNHfW6ldHnvckBie3OjeR?= MYLHTVUxKD1iNz6zJO69VQ>? MmS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOEGwNlMoRjJ4MEixNFI{RC:jPh?=
MCF7 MXzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NHXaZVZKSzVyIE2gO{4{OyEQvF2= NHjMPVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[0OFIyQSd-MkO2OFQzOTl:L3G+
A549 NGnRZW5EgXSxdH;4bYNqfHliYYPzZZk> NYjlVJFsXEeLIE2gO{4{PiEQvF2= M{niN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmwOVA5Lz5{NkW5NFUxQDxxYU6=
WI38 MmLpR5l1d3SxeHnjbZR6KGG|c3H5 M3vOSmlEPTBiPTC3MlU1KM7:TR?= M3\xPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEK2PFE2Lz5{NEiyOlgyPTxxYU6=
A375 M1LTfmN6fG:2b4jpZ4l1gSCjc4PhfS=> MmX6TWM2OCB;IEeuOVYh|ryP NXnaSXk3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNVU5OThpPkK0NlE2QDF6PD;hQi=>
MDA-MB-231 NETpUFdEgXSxdH;4bYNqfHliYYPzZZk> NIrn[o5KSzVyIE2gO{43OiEQvF2= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF6MUSxOEc,OjBzOEG0NVQ9N2F-
786-0 Mo\PR5l1d3SxeHnjbZR6KGG|c3H5 NFPFXndVT0liPTC3MlYzKM7:TR?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
HS27 MXnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M{fyZWlEPTBiPTC3Mlgh|ryP M4fNO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OEW3PVY{Lz5zOUi1O|k3OzxxYU6=
HS27 MVHDfZRwfG:6aXPpeJkh[XO|YYm= Moq1TWM2OCB;IEeuPEDPxE1? Mn;tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6OUezOlYoRjF7OEm3N|Y3RC:jPh?=
HCT116 M33XOGN6fG:2b4jpZ4l1gSCjc4PhfS=> NGO1OJFKSzVyIE2gO{45KM7:TR?= NULFZ2MxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0PFM2QTJpPkKyOFg{PTl{PD;hQi=>
HS27 Mm\ORY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MnnPTWM2OCB;IEeuPFUh|ryP NUPSSVJqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0OlQ5QDdpPkG5OFY1QDh5PD;hQi=>
HS27 M1[5eGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NXH4eWpQT0l3MDC9JFcvQDVizszN MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN3M{W3NUc,OjF|NUO1O|E9N2F-
A549/ATCC NYTucnZ{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYXJdWhjXEeLIE2gO{46PCEQvF2= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
DU145 MXnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHjnd5hVT0liPTC3Mlk1KM7:TR?= M3XnS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
NCI/ADR-RES NWLWZ4h{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3L2bnRIUSB;IEeuPVQh|ryP MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
MCF7 M1:4c2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVrrRo55XEeLIE2gO{46PCEQvF2= NXrOdW5qRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
PC3 MlmyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MUjUS2khRSB5Lkm0JO69VQ>? NVeweFdWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
UO31 NHvqWWFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MVzUS2khRSB5Lkm0JO69VQ>? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
NCI-ADR-RES M1nGdmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NX\Ob3VqXEeLIE2gO{46PCEQvF2= NXPhWFFyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
LOXIMVI M3;B[WFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MYjMR|UxKD1iNz65OEDPxE1? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
SK-MEL-28 M1zCN2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MlTkWGdKKD1iNz65OEDPxE1? M{HFclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
KM12 MVvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MkHKWGdKKD1iNz65OEDPxE1? MonTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
COLO205 M{\JXmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MYTUS2khRSB5Lkm0JO69VQ>? NYr6R3c{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
HT-29 MlnTRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MX3US2khRSB5Lkm0JO69VQ>? M1rQcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
NCI-H226 NVXvXVFQSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MnfSWGdKKD1iNz65OEDPxE1? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
HOP62 MnjURY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MWjUS2khRSB5Lkm0JO69VQ>? NHrIS|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
T47D NFfkPGpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MljiWGdKKD1iNz65OEDPxE1? M3rTOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
MDA-MB-468 NELTVmlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MYjUS2khRSB5Lkm0JO69VQ>? NVz2R28zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
EKVX M3fyfmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MkHXWGdKKD1iNz65OEDPxE1? MnuyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
MCF7 MWPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MlvuWGdKKD1iNz65OEDPxE1? M37rXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
Hs 578T NHLZeWxCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NHfVXlVVT0liPTC3Mlk1KM7:TR?= NUXhZVNORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
MGC803 M37IXmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M2P3bGlEPTBiPTC3Mlk6KM7:TR?= NHTYPYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW2NFA1QSd-Mk[1OlAxPDl:L3G+
HCT116 NHjlVJVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MmDrTWM2OCB;IEiuNFgh|ryP MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR2MES3PUc,OjR2NEC0O|k9N2F-
PC3 NGrkV|ZEgXSxdH;4bYNqfHliYYPzZZk> M3v2SmlEPTBiPTC4MlA5KM7:TR?= MlnqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyNEOyOlgoRjJ5MESzNlY5RC:jPh?=
HCT116 NF;XW4xCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MmjWTWM2OCB;IEiuNFgh|ryP MlWwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NESyNVkoRjJ|NkS0NlE6RC:jPh?=
WM266.4 NX;ZNpN[SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M{\zc2dKPTBiPTC4MlEh|ryP MmDoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMkW5PVYoRjJ|MEK1PVk3RC:jPh?=
WM266.4 Mn\mRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MnjYS2k2OCB;IEiuNVIh|ryP M{PBcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUizOlY6Lz5{MkW4N|Y3QTxxYU6=
Ketr3 NHLnfXBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MonRTWM2OCB;IEiuNlch|ryP MkOyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{MU[4OFkoRjNyMkG2PFQ6RC:jPh?=
A375 NIHtSJdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NXnPXYZxUUN3MDC9JFgvOjdizszN M{\oUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkG2PFQ6Lz5|MEKxOlg1QTxxYU6=
HepG2 M1vmV2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NGG3TYNKSzVyIE2gPE4zPyEQvF2= M2rPd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkG2PFQ6Lz5|MEKxOlg1QTxxYU6=
MX1 MVLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NIOz[pJKSzVyIE2gPE4zPyEQvF2= NEHDRnY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEKxOlg1QSd-M{CyNVY5PDl:L3G+
MX1 MkO5R5l1d3SxeHnjbZR6KGG|c3H5 M2j6OmlEPTBiPTC4MlI4KM7:TR?= NYPS[VZPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2O|UyOzVpPkK0Olc2OTN3PD;hQi=>
WM266.4 MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4rCe2dKPTBiPTC4MlMh|ryP MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN4MU[4Okc,OjJ|NkG2PFY9N2F-
PLC/PRF/5 MlztR5l1d3SxeHnjbZR6KGG|c3H5 NISwfo1KSzVyIE2gPE4{KM7:TR?= MnrUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3M{GwOVMoRjJzNUOxNFU{RC:jPh?=
A375 NUPWcFUySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NEjDeIFKSzVyIE2gPE4{OyEQvF2= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTh6NkOyOEc,Ojl6OE[zNlQ9N2F-
HCT116 MWTDfZRwfG:6aXPpeJkh[XO|YYm= NFK3RY5KSzVyIE2gPE41OSEQvF2= NGTDbZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[zNVc5QCd-Mkm2N|E4QDh:L3G+
WI38 Mm\BR5l1d3SxeHnjbZR6KGG|c3H5 MYLJR|UxKD1iOD60NkDPxE1? M2LPTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUmxPVM5Lz5{Nkm5NVk{QDxxYU6=
HepG2 MoHRR5l1d3SxeHnjbZR6KGG|c3H5 NHrlcGNKSzVyIE2gPE41OiEQvF2= MnrEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzNkKxNlMoRjJ5MU[yNVI{RC:jPh?=
HepG2 MlXHR5l1d3SxeHnjbZR6KGG|c3H5 MmjITWM2OCB;IEiuOFIh|ryP NFe5Zms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i3NVkxQSd-MkO4O|E6ODl:L3G+
HT-29 MmjXRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M2PxWGlEPTBiPTC4MlQ1KM7:TR?= MmDYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4MEK2O|QoRjJ7NkCyOlc1RC:jPh?=
HepG2 MmTSR5l1d3SxeHnjbZR6KGG|c3H5 MVTJR|UxKD1iOD62O{DPxE1? MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ5NUGzOUc,OjR4N{WxN|U9N2F-
MCF7 NHr4R3BEgXSxdH;4bYNqfHliYYPzZZk> M1i0TWlEPTBiPTC4Mlg{KM7:TR?= M2PC[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEO1OFc6Lz5{MESzOVQ4QTxxYU6=
MCF7 MX;DfZRwfG:6aXPpeJkh[XO|YYm= NX;meIhvUUN3MDC9JFkvOTJizszN MmPwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MEC5NlAoRjJ2M{CwPVIxRC:jPh?=
HepG2 NHLDUHlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MXfJR|UxKD1iOT6xOEDPxE1? MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV4MEC0PUc,OjZ3NkCwOFk9N2F-
A375 MXTDfZRwfG:6aXPpeJkh[XO|YYm= MXPJR|UxKD1iOT6xO{DPxE1? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ5NUGzOUc,OjR4N{WxN|U9N2F-
MGC803 NXLv[ppoSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NWPv[YtFUUN3MDC9JFkvOiEQvF2= Ml3rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyM{KwN|EoRjJ7MEOyNFMyRC:jPh?=
SKOV3 M4nEOWN6fG:2b4jpZ4l1gSCjc4PhfS=> MWDJR|UxKD1iOT6yOUDPxE1? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDNyMEmyNEc,OjR|MEC5NlA9N2F-
RS4:11 M4W2PWZ2dmO2aX;uJIF{e2G7 MYfHTVUxKD1iOT6zJO69VQ>? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MUCyN{c,OjZyOEGwNlM9N2F-
RS4:11 MYjDfZRwfG:6aXPpeJkh[XO|YYm= NVXuc3dYT0l3MDC9JFkvOyEQvF2= NUWycG04RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NVg4ODlpPkKzOlE5PzB7PD;hQi=>
MIAPaCa2 MkH2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M2\JS2lEPTBiPTC5MlMzKM7:TR?= M3vNNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MEOyNFMyLz5{OUCzNlA{OTxxYU6=
PANC1 MoPUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHvzNlJKSzVyIE2gPU4{OiEQvF2= NXS4VpRWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxNFIyPzVpPkK5NVAzOTd3PD;hQi=>
MHCC97H MmPyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGfRcVNKSzVyIE2gPU4{OiEQvF2= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTFyMkG3OUc,OjlzMEKxO|U9N2F-
RS4:11 MYLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MUTHTVUxKD1iOT60JO69VQ>? MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdyOES2PEc,OjF5MEi0Olg9N2F-
Hep3B M4rpNWN6fG:2b4jpZ4l1gSCjc4PhfS=> M3;vcmlEPTBiPTC5MlQh|ryP M{TaR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OE[1Nlc3Lz5{OEi2OVI4PjxxYU6=
LoVo MnH4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYrMZZE{UUN3MDC9JFkvPDdizszN MkfCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzMEKxO|UoRjJ7MUCyNVc2RC:jPh?=
SMMC7721 M{LxWWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MX3JR|UxKD1iOT65OkDPxE1? MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ|N{GyN{c,OjV4M{exNlM9N2F-
MCF7 NH;qTHJEgXSxdH;4bYNqfHliYYPzZZk> MnzYWGdKKD1iOT65O{DPxE1? NFvXNlU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
IGROV1 MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHjJfYdVT0liPTCxNEDPxE1? M3HSZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
ACHN MlL4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MU\US2khRSBzMDFOwG0> NH24VJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
TK10 M3HHWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2XtNHRIUSB;IEGwJO69VQ>? NXHWcVJzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
786-0 M13zWWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NUfGT2pHXEeLIE2gNVAh|ryP MkHRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
SN12C NFHY[4pCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NWLYV4xnXEeLIE2gNVAh|ryP NF;VNpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
Caki1 Ml76RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? Mnn0UGM2OCB;IEGwJO69VQ>? NEXwdVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
TK10 NIjVe5dCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MnfyWGdKKD1iMUCg{txO MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
RXF393 NFW0SWNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NYfhNlNOXEeLIE2gNVAh|ryP M3izXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
OVCAR8 M{LWSWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MWHUS2khRSBzMDFOwG0> M4TnXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
SNB75 NFzv[WNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M2C0ZnRIUSB;IEGwJO69VQ>? NUfjS41LRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
NCI-H322M NEnNZ3VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M4L3O3RIUSB;IEGwJO69VQ>? NEjINmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
MDA-MB-435 NIDRS|dCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MoTKUGM2OCB;IEGwJO69VQ>? MlnxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
SF539 M2\JdGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NILleWZVT0liPTCxNEDPxE1? M{DrSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
SF268 NUf4PGR3SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MX\US2khRSBzMDFOwG0> NGTtOnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
NCI-H460 M3nFRWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NWDNenpVXEeLIE2gNVAh|ryP MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
DU145 MX\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MlX2WGdKKD1iMUCg{txO NYS5N4dqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
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Bel7402 MmXyR5l1d3SxeHnjbZR6KGG|c3H5 M{P4W2lEPTBiPTCxNE4zPiEQvF2= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVyNEKwOEc,OjF3MESyNFQ9N2F-
Bel7402 MlruS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2DndGlEPTBiPTCxNE4{OSEQvF2= NFnV[WU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUGwNlE4PSd-MkmxNFIyPzV:L3G+
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SGC7901 M2LNO2N6fG:2b4jpZ4l1gSCjc4PhfS=> MoHFTWM2OCB;IEGwMlM4KM7:TR?= M4XWc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEO1OFc6Lz5{MESzOVQ4QTxxYU6=
SNB75 NH\PUXFEgXSxdH;4bYNqfHliYYPzZZk> NWD4b2hqXEeLIE2gNVAvPDJizszN M2DhbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmwOVA5Lz5{NkW5NFUxQDxxYU6=
A431 NGjHVWlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NFuwcIRKSzVyIE2gNVAvPDZizszN M2rpXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MEOyNFMyLz5{OUCzNlA{OTxxYU6=
SMMC7721 NF\rWmlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MnKyTWM2OCB;IEGwMlYyKM7:TR?= NFruXVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUCzNlA{OSd-MkmwN|IxOzF:L3G+
GES-1 MXTDfZRwfG:6aXPpeJkh[XO|YYm= MYfJR|UxKD1iMUCuOlgh|ryP NH3GRpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G2NlEzOyd-MkexOlIyOjN:L3G+
H460 MVfDfZRwfG:6aXPpeJkh[XO|YYm= NF:zOHhKSzVyIE2gNVAvQCEQvF2= Ml7WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MEC5NlAoRjJ2M{CwPVIxRC:jPh?=
BGC823 MVnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MlzoTWM2OCB;IEGwMlkyKM7:TR?= NVzncnBpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3NlE6OjRpPkKyO|IyQTJ2PD;hQi=>
Hep3B M1PoPGN6fG:2b4jpZ4l1gSCjc4PhfS=> NWHJ[Y9ZUUN3MDC9JFEyNjJizszN MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDN2MkSwNEc,Ojh|NEK0NFA9N2F-
MCF7 MVfDfZRwfG:6aXPpeJkh[XO|YYm= M17Be2lEPTBiPTCxNU4{PCEQvF2= MoLGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzNkKxNlMoRjJ5MU[yNVI{RC:jPh?=
MCF7 MljPR5l1d3SxeHnjbZR6KGG|c3H5 MnXITWM2OCB;IEGxMlM1KM7:TR?= NFrjW4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i3NVkxQSd-MkO4O|E6ODl:L3G+
SK-MEL-2 NGfXdFlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBpfW2jbh?= Mlz0TWM2OCB;IEGxMlM2KM7:TR?= M4f4elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkSyOVU{Lz5{OEK0NlU2OzxxYU6=
HepG2 MV3DfZRwfG:6aXPpeJkh[XO|YYm= M3XiOmlEPTBiPTCxNU41QSEQvF2= MlHVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MESyNFQoRjJzNUC0NlA1RC:jPh?=
A375P NX7EW4ptSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NUm0ZXRyUUN3MDC9JFEyNjVizszN MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjBzNEW1PUc,OjJyMUS1OVk9N2F-
SGC7901 M3W1[GN6fG:2b4jpZ4l1gSCjc4PhfS=> Mme3TWM2OCB;IEGxMlUh|ryP M2DZ[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{CwPVIxLz5{NEOwNFkzODxxYU6=
HeLa MkDTRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NV[1[WR6UUN3MDC9JFEzNjBzIN88US=> M{TPPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUCzPFc{Lz5{OUGwN|g4OzxxYU6=
PANC1 NED6RXBEgXSxdH;4bYNqfHliYYPzZZk> MkXHTWM2OCB;IEGyMlMh|ryP NFT6VGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOwNFkzOCd-MkSzNFA6OjB:L3G+
HepG2 NVS3VVE4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MlrtTWM2OCB;IEGyMlU1KM7:TR?= MonSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyM{KwN|EoRjJ7MEOyNFMyRC:jPh?=
A549 NILKfVBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M4fDRmlEPTBiPTCxNk42PCEQvF2= M4nUdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkS0NlE6Lz5{M{[0OFIyQTxxYU6=
Caki1 NYrOVmlwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWDZe|hVXEeLIE2gNVIvPTlizszN NXq0fWRlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
OVCAR8 NXv0eGVYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NITwcGtVT0liPTCxNk42QSEQvF2= M3e1[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
SKOV3 NH\tWINCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NVLudFR7XEeLIE2gNVIvPiEQvF2= NVji[5RrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
ACHN NEPHdVFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= Moq3WGdKKD1iMUKuOkDPxE1? NGHG[|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
OVCAR3 MlTqRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MVfUS2khRSBzMj62JO69VQ>? NVzMXJhnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
OVCAR4 MkDoRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MlzvWGdKKD1iMUKuOkDPxE1? MmLwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
HCT15 M4D1NmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MYLUS2khRSBzMj62JO69VQ>? NXzQXZJbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
SW620 NYLCdpNbSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M{f4[3RIUSB;IEGyMlYh|ryP MmT1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
A549/ATCC NGPXSYtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MYLUS2khRSBzMj62JO69VQ>? M1fsNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
MDA-MB-468 MYDDfZRwfG:6aXPpeJkh[XO|YYm= NEPSNYlVT0liPTCxN{4xQSEQvF2= NXPXd3J6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
SK-MEL-28 NXnVZppPS3m2b4TvfIlkcXS7IHHzd4F6 Mk\KWGdKKD1iMUOuN|kh|ryP NWHN[FlERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
A549 NHrRUZZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MUDJR|UxKD1iMUOuOlQh|ryP M2rROVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NU[wNFQ6Lz5{NkW2NFA1QTxxYU6=
A375 M4PBcGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIh2dWGw NYHhWmVrUUN3MDC9JFE{NjZ2IN88US=> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ2MkW1N{c,Ojh{NEK1OVM9N2F-
A375 NETTSIZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MW\JR|UxKD1iMUOuOlQh|ryP NEDC[5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS2NlI3Pyd-MkW0OlIzPjd:L3G+
K562 M3z5eGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NYjMcZIyUUN3MDC9JFE{Njh3IN88US=> NIn2b5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[0OFIyQSd-MkO2OFQzOTl:L3G+
Hep3B M4HpXGN6fG:2b4jpZ4l1gSCjc4PhfS=> MWjJR|UxKD1iMUSuNFgh|ryP NXXUd29LRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NFQzODRpPkKxOVA1OjB2PD;hQi=>
T47D NH7VRW1EgXSxdH;4bYNqfHliYYPzZZk> MnGyWGdKKD1iMUSuN|gh|ryP MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
NCI-H226 NELKeWdEgXSxdH;4bYNqfHliYYPzZZk> M4nwcnRIUSB;IEG0MlQzKM7:TR?= M3PVWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmwOVA5Lz5{NkW5NFUxQDxxYU6=
MDA-MB-231 M3vzbWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MnL6TWM2OCB;IEG0MlYzKM7:TR?= NWTvcJJURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OFA1PzlpPkK0OFQxPDd7PD;hQi=>
MDA-MB-231 NXrMPIlxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NX;XPVhlUUN3MDC9JFE1NjZ{IN88US=> MnTyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NESyNVkoRjJ|NkS0NlE6RC:jPh?=
NCI-H522 NV;HWFhES3m2b4TvfIlkcXS7IHHzd4F6 M{TkS3RIUSB;IEG0MlgzKM7:TR?= NF3Eb|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
SK-MEL-2 Ml;sR5l1d3SxeHnjbZR6KGG|c3H5 M33xSHRIUSB;IEG0Mlg2KM7:TR?= MlHLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
OS-RC2 NUfUOmpYS3m2b4TvfIlkcXS7IHHzd4F6 MYLJR|UxKD1iMUWg{txO NHPRUlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe4OlQ{Pid-Mkm3PFY1OzZ:L3G+
HepG2 MlP0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NGj1[5JKSzVyIE2gNVUh|ryP NV\3UFRIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxNFM5PzNpPkK5NVA{QDd|PD;hQi=>
fibroblast MW\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NV3VWINDUUN3MDC9JFE2NjFizszN M4DHW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEe3OVA2Lz5zOES3O|UxPTxxYU6=
HT-29 MYLDfZRwfG:6aXPpeJkh[XO|YYm= NH7PSVdKSzVyIE2gNVUvOiEQvF2= Ml3YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4N{WxN|UoRjJ2Nke1NVM2RC:jPh?=
U87MG NW\xbm9wS3m2b4TvfIlkcXS7IHHzd4F6 NUjISFdoUUN3MDC9JFE2NjV5IN88US=> NHzMVFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEiyOlgyPSd-MkS4NlY5OTV:L3G+
BT549 MWPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MmDhUGM2OCB;IEG1Mlgh|ryP MoD6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
GES-1 MVzDfZRwfG:6aXPpeJkh[XO|YYm= NGmxWYRKSzVyIE2gNVUvQCEQvF2= NWX3NGpvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4O|E6ODlpPkKzPFcyQTB7PD;hQi=>
SNB19 NUj6bWJpSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MlvFWGdKKD1iMUWuPFUh|ryP MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
IGROV1 M1\mOWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MVnUS2khRSBzNT65JO69VQ>? NV7lUpR6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
HCT116 M3;SV2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MWXMR|UxKD1iMUWuPUDPxE1? M2fSW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
OS-RC2 Mn\kR5l1d3SxeHnjbZR6KGG|c3H5 Mo\sTWM2OCB;IEG2JO69VQ>? NWfGNpJWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVQxOTdpPkKzOFU1ODF5PD;hQi=>
A375 NWq1Zmd6S3m2b4TvfIlkcXS7IHHzd4F6 M3f3dmlEPTBiPTCxOk4zPCEQvF2= NWDPWmZjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OlE{OThpPkK1OFYyOzF6PD;hQi=>
SKOV3 NEH1Z|hEgXSxdH;4bYNqfHliYYPzZZk> MYPUS2khRSBzNj6zOkDPxE1? NEPjc2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
WM1361 Moj0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MofiTWM2OCB;IEG2MlQ1KM7:TR?= M1;CSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkCyOlc1Lz5{OU[wNlY4PDxxYU6=
MKN28 NGrCV3JCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NYnnXmozUUN3MDC9JFE4KM7:TR?= MkPaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyM{KwN|EoRjJ7MEOyNFMyRC:jPh?=
SMMC7721 NIjUTo1EgXSxdH;4bYNqfHliYYPzZZk> NGfZUIZKSzVyIE2gNVcvOyEQvF2= NYS4b21CRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NFQzODRpPkKxOVA1OjB2PD;hQi=>
UACC257 NGfRcWhEgXSxdH;4bYNqfHliYYPzZZk> NUX3NIkxXEeLIE2gNVcvOzNizszN NXXuNmg{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
EAhy926 MUTBcpRq[W6paX;n[Y5q[yCjY4Tpeol1gSCjc4PhfS=> Mm\WTWM2OCB;IEG4MlUzKM7:TR?= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB|MkCzNUc,OjlyM{KwN|E9N2F-
SMMC7721 M17RemN6fG:2b4jpZ4l1gSCjc4PhfS=> MYLJR|UxKD1iMUiuO{DPxE1? M2[4O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{CwPVIxLz5{NEOwNFkzODxxYU6=
Ketr3 MYfDfZRwfG:6aXPpeJkh[XO|YYm= Mk\oTWM2OCB;IEG4Mlgh|ryP M4\2T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Nke1NVM2Lz5{NE[3OVE{PTxxYU6=
NCI-H522 M1rVUGN6fG:2b4jpZ4l1gSCjc4PhfS=> M{XwZWlEPTBiPTCxPU4zPiEQvF2= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MUOxPEc,OjV2NkGzNVg9N2F-
A549 MmD0R5l1d3SxeHnjbZR6KGG|c3H5 Ml7YTWM2OCB;IEG5MlU1KM7:TR?= M1TNW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MU[yNVI{Lz5{N{G2NlEzOzxxYU6=
SH-SY5Y NHz0eJhEgXSxdH;4bYNqfHliYYPzZZk> MlXKTWM2OCB;IEG5MlU1KM7:TR?= Mmf1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6N{G5NFkoRjJ|OEexPVA6RC:jPh?=
MGC803 M{C2VGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M4HqPGlEPTBiPTCxPU46OiEQvF2= M2HvbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUCzPFc{Lz5{OUGwN|g4OzxxYU6=
EKVX NFrweZlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1vwV3RIUSB;IEG5Mlk2KM7:TR?= MoLqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
OVCAR5 MmC4RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NWHlPWhGXEeLIE2gNlAh|ryP MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
UACC62 MnfqRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M1jMdGxEPTBiPTCyNEDPxE1? MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
UACC257 MonIRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NGfLOJhNSzVyIE2gNlAh|ryP NXL0OpJxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
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B16-BL6 NGe0fmREgXSxdH;4bYNqfHliYYPzZZk> Mn63TWM2OCB;IEO2MlQ5KM7:TR?= NWrrSVdVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2N|E4QDhpPkK5OlMyPzh6PD;hQi=>
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
LC3-I / LC-3II / ATG5; 

PubMed: 23392173     


Sorafenib induces the conversion of LC3 in a dose-dependent manner. PLC5, Hep3B, SK-Hep1 and HepG2 were exposed to sorafenib at the indicated doses for 16 h and the expression levels of LC3-II were analyzed by western blot.

p-STAT3 / STAT3 / Mcl-1; 

PubMed: 23392173     


Effects of sorafenib on STAT3-related proteins in HCC cells. The cells were treated with sorafenib at the indicated dose for 16 h. 

β-catenin / Survivin / Mcl-1 / PTMA; 

PubMed: 26517516     


Co-inhibition of β-catenin and PTMA by sorafenib in HCC cells. Cell lines indicated on top were treated or not with 10 μM sorafenib for 24 hrs and processed for immuno-blotting. IC50 values (the concentration of sorafenib that inhibits 50% of cell growth) for each cell line are indicated below the panels.

pERK / ERK; 

PubMed: 22286758     


Western blot analysis of p-ERK (T202/Y204) and ERK at indicated time points in HCT116 cells treated with 20 μmol/L sorafenib.

p-PKM2(y105) / PMK2 / Caspase-9; 

PubMed: 26959741     


Sorafenib downregulates the p-PKM2 (Y105) at the indicated doses after treatment for 24 h.

RET(pY1016) / VEGFR2(pY1214) / MEK1(pT292) / ERK(pY204); 

PubMed: 22941289     


Sorafenib affected the phosphorylation of receptor tyrosine kinase RET and VEGFR2, as well as MEK/ERK kinases signaling cascades in three cell lines. Three cell lines were treated by sorafenib with two concentration gradients, 1 and 5 μmol/L/L, and then collected after 2, 4, and 8 h, cells without sorafenib treatment were as the controls (0 h). Total proteins were extracted and quantified to be used in Western blot assays. (A) Sorafenib inhibited RET and VEGFR2 phosphorylation dose-dependently while activated MEK and ERK phosphorylation in A549 cells. (B) Sorafenib also inhibited RET and VEGFR2 phosphorylation, and slightly activated MEK and ERK phosphorylation in HeLa cells. (C) Sorafenib activated the phosphorylation of RET, VEGFR2, and MEK, but inhibited ERK phosphorylation in HepG2 cells.

Cyclin D1; 

PubMed: 26039995     


Dose-escalation effects of sorafenib or SC-1 for 24 h on STAT3-related proteins in HSC-T6 and LX2 cells.

23392173 26517516 22286758 26959741 22941289 26039995
Growth inhibition assay
Cell viability; 

PubMed: 26039995     


Dose-escalation and time-dependent effects of sorafenib for 24 or 48 h on cell viability in HSC-T6, LX2, and mouse primary HSCs. Circles, mean; bars, SE (n = 3).

26039995
Immunofluorescence
p65; 

PubMed: 22286758     


HCT116 cells were treated with 20 μmol/L sorafenib or 10 ng/mL TNF-α for 3 hours then fixed. Immunofluorescence was carried out as described in the Materials and Methods for p65 (green) and DAPI (blue). Representative pictures (400×) are shown.

cytochrome c; 

PubMed: 22278289     


Immunoflourescent staining and quantification of mitochondrial membrane potential (appearing in red, mitotracker) and cytochrome c (appearing in green, FITC) in 22Rv1 and PC3 treated with 20 μM sorafenib for 24 h.

22286758 22278289
ELISA
TGF-beta / CD206; 

PubMed: 26158762     


In Macrophage, TGF-β secretion and CD206 were confirmed by ELISA.

Caspase-9 / Caspase-3; 

PubMed: 30923462     


Caspase-9 and caspase-3 activities were measured via ELISA assay. FCCP, an activator of mitophagy, was added into the medium of sorafenib-treated cells to activate mitophagy. Adenovirus-loaded LATS2 (Ad-LATS2) was transfected into HepG2 cells in the presence of sorafenib. *p < 0.05 vs. control group; #p < 0.05 vs. Sorafenib + Ad-cont group; #p < 0.05 vs. Sorafenib + Ad-LATS2 group.

26158762 30923462
In vivo Oral administration of Sorafenib (~60 mg/kg) demonstrates broad spectrum, dose-dependent anti-tumor activity against a variety of human tumor xenograft models including MDA-MB-231, Colo-205, HT-29, DLD-1, NCI-H460, and A549, with no evidence of toxicity. In association with the anti-tumor efficacy, Sorafenib treatment potently inhibits MEK 1/2 phosphorylation and pERK 1/2 levels in HT-29 and MDA-MB-231 xenografts but not in Colo-205 xenografts, and significantly suppresses tumor microvessel area (MVA) and microvessel density (MVD) in MDA MB-231, HT-29 and Colo-205 tumor xenografts. [1] Sorafenib treatment produces dose-dependent growth inhibition of PLC/PRF/5 tumor xenografts in SCID mice with TGIs of 49% and 78% at 10 mg/kg and 30 mg/kg, respectively, consistent with the inhibition of ERK and eIF4E phosphorylation, reduction of the microvessel area, and induction of tumor cell apoptosis. [2] Sorafenib sensitizes bax-/- cells to TRAIL in a dose-dependent manner, through a mechanism involving down-regulating NF-κB mediated Mcl-1 and cIAP2 expression. Combining Sorafenib (30-60 mg/kg) with TRAIL (5 mg/kg) show dramatic efficacy in TRAIL-resistant HCT116 bax-/- and HT29 tumor xenografts. [3]

Protocol

Kinase Assay:

[1]

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Biochemical assays:

Recombinant baculoviruses expressing Raf-1 (residues 305–648) and B-Raf (residues 409–765) are purified as fusion proteins. Full-length human MEK-1 is generated by PCR and purified as a fusion protein from Escherichia coli lysates. Sorafenib tosylate is added to a mixture of Raf-1 (80 ng), or B-Raf (80 ng) with MEK-1 (1 μg) in assay buffer [20 mM Tris (pH 8.2), 100 mM NaCl, 5 mM MgCl2, and 0.15% β-mercaptoethanol] at a final concentration of 1% DMSO. The Raf kinase assay (final volume of 50 μL) is initiated by adding 25 μL of 10 μM γ[33P]ATP (400 Ci/mol) and incubated at 32 °C for 25 minutes. Phosphorylated MEK-1 is harvested by filtration onto a phosphocellulose mat, and 1% phosphoric acid is used to wash away unbound radioactivity. After drying by microwave heating, a β-plate counter is used to quantify filter-bound radioactivity. Human VEGFR2 (KDR) kinase domain is expressed and purified from Sf9 lysates. Time-resolved fluorescence energy transfer assays for VEGFR2 are performed in 96-well opaque plates in the time-resolved fluorescence energy transfer format. Final reaction conditions are as follows: 1 to 10 μM ATP, 25 nM poly GT-biotin, 2 nM Europium-labeled phospho (p)-Tyr antibody (PY20), 10 nM APC, 1 to 7 nM cytoplasmic kinase domain in final concentrations of 1% DMSO, 50 mM HEPES (pH 7.5), 10 mM MgCl2, 0.1 mM EDTA, 0.015% Brij-35, 0.1 mg/mL BSA, and 0.1% β-mercaptoethanol. Reaction volumes are 100 μL and are initiated by addition of enzyme. Plates are read at both 615 and 665 nM on a Perkin-Elmer VictorV Multilabel counter at ~1.5 to 2.0 hours after reaction initiation. Signal is calculated as a ratio: (665 nm/615 nM) × 10,000 for each well. For IC50 generation, Sorafenib tosylate is added before the enzyme initiation. A 50-fold stock plate is made with Sorafenib tosylate serially diluted 1:3 in a 50% DMSO/50% distilled water solution. Final Sorafenib tosylate concentrations range from 10 μM to 4.56 nM in 1% DMSO.
Cell Research:

[1]

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  • Cell lines: MDA-MB-231, and HAoSMC
  • Concentrations: Dissolved in DMSO, final concentrations ~10 μM
  • Incubation Time: 72 hours
  • Method:

    Cells are exposed to increasing concentrations of Sorafenib tosylate for 72 hours. Cell number is quantitated using the Cell TiterGlo ATP Luminescent assay kit. This assay measures the number of viable cells per well by measurement of luminescent signal based on amount of cellular ATP.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Female NCr-nu/nu mice implanted s.c. with MDA-MB-231, Colo-205, HT-29, H460, or A549 cells
  • Dosages: ~60 mg/kg
  • Administration: Orally once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 63 mg/mL warmed (135.53 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5%DMSO+45%PEG400+50%H2O
For best results, use promptly after mixing.
0.375mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 464.82
Formula

C21H16ClF3N4O3

CAS No. 284461-73-0
Storage powder
in solvent
Synonyms NSC-724772
Smiles CNC(=O)C1=NC=CC(=C1)OC2=CC=C(C=C2)NC(=O)NC3=CC(=C(C=C3)Cl)C(F)(F)F

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04710641 Not yet recruiting Drug: MTL-CEBPA|Drug: Sorafenib Hepatocellular Carcinoma|Hepatitis B|Hepatitis C Mina Alpha Limited May 1 2021 Phase 2
NCT04763408 Not yet recruiting Drug: Lenvatinib|Drug: Sorafenib Carcinoma Hepatocellular Eisai Inc. March 15 2021 --
NCT04000737 Recruiting Drug: YIV-906+Sorafenib|Drug: Placebo+Sorafenib Advanced Hepatocellular Carcinoma Yiviva Inc. January 10 2020 Phase 2
NCT03958669 Recruiting -- Hepatocellular Carcinoma|Sorafenib University Hospital Tuebingen|German Federal Ministry of Education and Research November 1 2019 --
NCT03764293 Recruiting Drug: SHR-1210|Drug: Apatinib|Drug: Sorafenib Locally Advanced or Metastatic and Unresectable HCC Jiangsu HengRui Medicine Co. Ltd. June 10 2019 Phase 3
NCT03582618 Recruiting Drug: Sorafenib|Drug: CVM-1118 Hepatocellular Carcinoma|Advanced Cancer TaiRx Inc. July 12 2018 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Raf Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID