Sorafenib

For research use only.

Catalog No.S7397 Synonyms: BAY 43-9006

336 publications

Sorafenib Chemical Structure

CAS No. 284461-73-0

Sorafenib (BAY 43-9006) is a multikinase inhibitor of Raf-1 and B-Raf with IC50 of 6 nM and 22 nM in cell-free assays, respectively. Sorafenib inhibits VEGFR-2, VEGFR-3, PDGFR-β, Flt-3 and c-KIT with IC50 of 90 nM, 20 nM, 57 nM, 59 nM and 68 nM, respectively. Sorafenib induces autophagy and apoptosis and activates ferroptosis with anti-tumor activity.

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Selleck's Sorafenib has been cited by 336 publications

Purity & Quality Control

Choose Selective Raf Inhibitors

Biological Activity

Description Sorafenib (BAY 43-9006) is a multikinase inhibitor of Raf-1 and B-Raf with IC50 of 6 nM and 22 nM in cell-free assays, respectively. Sorafenib inhibits VEGFR-2, VEGFR-3, PDGFR-β, Flt-3 and c-KIT with IC50 of 90 nM, 20 nM, 57 nM, 59 nM and 68 nM, respectively. Sorafenib induces autophagy and apoptosis and activates ferroptosis with anti-tumor activity.
Targets
Raf-1 [1]
(Cell-free assay)
mVEGFR2(Flk1) [1]
(Cell-free assay)
mVEGFR3 [1]
(Cell-free assay)
B-Raf [1]
(Cell-free assay)
B-Raf (V599E) [1]
(Cell-free assay)
6 nM 15 nM 20 nM 22 nM 38 nM
In vitro

Sorafenib inhibits both wild-type and V599E mutant B-Raf activity with IC50 of 22 nM and 38 nM, respectively. Sorafenib also potently inhibits mVEGFR2 (Flk-1), mVEGFR3, mPDGFRβ, Flt3, and c-Kit with IC50 of 15 nM, 20 nM, 57 nM, 58 nM, and 68 nM, respectively. Sorafenib weakly inhibits FGFR-1 with IC50 of 580 nM. Sorafenib tosylate is not active against ERK-1, MEK-1, EGFR, HER-2, IGFR-1, c-Met, PKB, PKA, cdk1/cyclinB, PKCα, PKCγ, and pim-1. Sorafenib markedly inhibits VEGFR2 phosphorylation in NIH 3T3 cells with IC50 of 30 nM, and Flt-3 phosphorylation in HEK-293 cells with IC50 of 20 nM. Sorafenib potently blocks MEK 1/2 and ERK 1/2 phosphorylation in most cell lines but not in A549 or H460 cells, while having no effect on inhibition of the PKB pathway. Sorafenib inhibits the proliferation of HAoSMC and MDA-MB-231 cells with IC50 of 0.28 μM and 2.6 μM, respectively. [1] In addition to inhibition of the RAF/MEK/ERK signaling pathway, Sorafenib significantly inhibits the phosphorylation of eIF4E and down-regulates Mcl-1 levels in hepatocellular carcinoma (HCC) cells in a MEK/ERK-independent manner. Sorafenib inhibits the proliferation of PLC/PRF/5 and HepG2 cells with IC50 of 6.3 μM and 4.5 μM, respectively, and leads to the significant induction of apoptosis. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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BB65-RCC MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxRTBwNEewO|Mh|ryP MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxN|M3Nyd-U1HOS2VTRC:jPh?=
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MDA-MB-231 MoDYR5l1d3SxeHnjbZR6KGG|c3H5 M{[0RWlEPTBiPTCwMlk1KM7:TR?= NVfsXWJpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwPFYzOzhpPkK1NFg3OjN6PD;hQi=>
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SNB75 NXfFcndnSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MYTHTVUxKD1iMz6xOkDPxE1? NFz6O3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
OVCAR3 M{fLemFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NWLjNGNHT0l3MDC9JFMvOTZizszN MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
SNB19 MX\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M2rp[mdKPTBiPTCzMlE3KM7:TR?= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
SK-MEL-5 M3jHUmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NH;KT2NVT0liPTCzMlE3KM7:TR?= NVq4Uo5JRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
OVCAR4 NHj5WppCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MlG4S2k2OCB;IEOuNVYh|ryP MmiwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
SK-MEL-2 MkPkRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NXu0eIFvXEeLIE2gN{4yPiEQvF2= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
BT549 NGPlS|VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MVPHTVUxKD1iMz6xOkDPxE1? NYDBO5lNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
MOLT4 NVLaXZlPSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MnH5S2k2OCB;IEOuNVYh|ryP M{T0fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
K562 NE\pOG1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NGn3XWdIUTVyIE2gN{4yPiEQvF2= NWPuRmJFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
HCC2998 NH7tWJRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NIrmSYZIUTVyIE2gN{4yPiEQvF2= MkH5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
HCC2998 M{jydWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M2LuNXRIUSB;IEOuNVYh|ryP NGjxfI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
A549/ATCC NEDwXlVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NVr4VZVuT0l3MDC9JFMvOTZizszN NYnnd5ltRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
DU145 MlPWRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M3;RV2dKPTBiPTCzMlE3KM7:TR?= NF7n[289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
SR NH;BOI1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M3u5ZWdKPTBiPTCzMlE3KM7:TR?= NIXPWm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
MCF7 NEXQNItEgXSxdH;4bYNqfHliYYPzZZk> MnywTWM2OCB;IEOuNVgh|ryP MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl{N{iwNUc,Ojh7Mke4NFE9N2F-
PC3 MoTCR5l1d3SxeHnjbZR6KGG|c3H5 MUfJR|UxKD1iMz6xPEDPxE1? NHXXW5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEmyO|gxOSd-Mki5Nlc5ODF:L3G+
MCF7 MnvZR5l1d3SxeHnjbZR6KGG|c3H5 NXnt[ZNbUUN3MDC9JFMvOThizszN M{DxV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{e3NFA6Lz5{N{e3O|AxQTxxYU6=
A549 NHL3N2lCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M4TWeGlEPTBiPTCzMlE6KM7:TR?= M2T4T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{e4PVk2Lz5{NUe3PFk6PTxxYU6=
PC3 M2fjUWN6fG:2b4jpZ4l1gSCjc4PhfS=> MUnJR|UxKD1iMz6yOEDPxE1? NWLnPVJQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke3O|cxODlpPkK3O|c4ODB7PD;hQi=>
HeLa NUG0SHFFTnWwY4Tpc44h[XO|YYm= MXTJR|UxKD1iMz6zJO69VQ>? MmPaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV{MkW3NFYoRjF3MkK1O|A3RC:jPh?=
HeLa MXjGeY5kfGmxbjDhd5NigQ>? M{HPUWlEPTBiPTCzMlMh|ryP NYLK[GJXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWyNlU4ODZpPkG1NlI2PzB4PD;hQi=>
LoVo M4nCNGN6fG:2b4jpZ4l1gSCjc4PhfS=> M3XqO2lEPTBiPTCzMlMh|ryP NUG0[FVwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVQxOTdpPkKzOFU1ODF5PD;hQi=>
A375 MYrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? Mn7kTWM2OCB;IEOuN|Yh|ryP NGCzO|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[wNlY4PCd-Mkm2NFI3PzR:L3G+
HT-29 M4rqR2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NW\tOJh1UUN3MDC9JFMvOzdizszN NEjJTY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm5NVk{QCd-Mk[5PVE6Ozh:L3G+
FLT3 gene-deficient U937 NEfKXXlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NUOw[W0yT0l3MDC9JFMvPCEQvF2= Mo[zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MEi0OlgoRjJzN{C4OFY5RC:jPh?=
FLT3 negative U937 MX7DfZRwfG:6aXPpeJkh[XO|YYm= M2rHb2dKPTBiPTCzMlQh|ryP NXTib|ZGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NVg4ODlpPkKzOlE5PzB7PD;hQi=>
U937 MWLDfZRwfG:6aXPpeJkh[XO|YYm= MYTHTVUxKD1iMz60JO69VQ>? MoryQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOEGwNlMoRjJ4MEixNFI{RC:jPh?=
A375P Ml3QRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NEj5fJVIUTVyIE2gN{41KM7:TR?= M2G1d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEW5NVQ1Lz5{OUS1PVE1PDxxYU6=
ACHN MXHDfZRwfG:6aXPpeJkh[XO|YYm= NX3scplSUUN3MDC9JFMvPCEQvF2= M{O0dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUG3PVA5Lz5{OUWxO|kxQDxxYU6=
ACHN NGnpW|NEgXSxdH;4bYNqfHliYYPzZZk> MVzJR|UxKD1iMz60JO69VQ>? NGTtcmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUK5O|Y5QCd-MkmyPVc3QDh:L3G+
HepG2 MXnGeY5kfGmxbjDhd5NigQ>? MoHvTWM2OCB;IEOuOEDPxE1? Mme5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyN{G4OlEoRjJ4MEexPFYyRC:jPh?=
A375P MkPGR5l1d3SxeHnjbZR6KGG|c3H5 MYXHTVUxKD1iMz60JO69VQ>? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh5OEG5N{c,OjR6N{ixPVM9N2F-
HepG2 NV63ZpNTS3m2b4TvfIlkcXS7IHHzd4F6 NHvRW|lKSzVyIE2gN{41PCEQvF2= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl{N{iwNUc,Ojh7Mke4NFE9N2F-
HepG2 M1fJTmN6fG:2b4jpZ4l1gSCjc4PhfS=> MUXJR|UxKD1iMz60OEDPxE1? M3W2[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{e3NFA6Lz5{N{e3O|AxQTxxYU6=
ACHN M2P4O2N6fG:2b4jpZ4l1gSCjc4PhfS=> M{PadmlEPTBiPTCzMlUh|ryP MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd6NkSzOkc,Ojl5OE[0N|Y9N2F-
HepG2 MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NVe2T2piUUN3MDC9JFMvPSEQvF2= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MkK2OUc,OjV2NkKyOlU9N2F-
HT-29 M4\IdGN6fG:2b4jpZ4l1gSCjc4PhfS=> NX\D[IdRUUN3MDC9JFMvPjFizszN MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh{NkixOUc,OjR6Mk[4NVU9N2F-
HT-29 M{LxWmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MoroTWM2OCB;IEOuOlEh|ryP M2\4V|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkG2PFQ6Lz5|MEKxOlg1QTxxYU6=
HT-29 MUHDfZRwfG:6aXPpeJkh[XO|YYm= MkPqTWM2OCB;IEOuOlEh|ryP Mn;GQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyOE[yN|goRjJ3MEi2NlM5RC:jPh?=
HT-29 MYHDfZRwfG:6aXPpeJkh[XO|YYm= MVPJR|UxKD1iMz62NUDPxE1? MkDWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NEC4O|koRjJ3NESwPFc6RC:jPh?=
MCF7 MUPBcpRq[2GwY3XyJIFkfGm4aYT5JIF{e2G7 Mn\ZTWM2OCB;IEOuOlQh|ryP MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV2OUi0NUc,Ojl3NEm4OFE9N2F-
Sf9 MV\GeY5kfGmxbjDhd5NigQ>? NWLTZXBVUUN3MDC9JFMvQCEQvF2= Mk\5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MEi0OlgoRjJzN{C4OFY5RC:jPh?=
Sf9 NF75NYlHfW6ldHnvckBie3OjeR?= MWnJR|UxKD1iMz64JO69VQ>? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZzOEewPUc,OjN4MUi3NFk9N2F-
Sf9 NV;pZ4I4TnWwY4Tpc44h[XO|YYm= MUTJR|UxKD1iMz64JO69VQ>? Ml\MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOEGwNlMoRjJ4MEixNFI{RC:jPh?=
A2058 M3HXXmN6fG:2b4jpZ4l1gSCjc4PhfS=> MXzJR|UxKD1iMz64JO69VQ>? MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjdyOEm4O{c,OjJ5MEi5PFc9N2F-
NCI-H460 M1ToeGN6fG:2b4jpZ4l1gSCjc4PhfS=> MorjTWM2OCB;IEOuPUDPxE1? MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR3NECxO{c,OjN2NUSwNVc9N2F-
SK-MEL-30 MkHVR5l1d3SxeHnjbZR6KGG|c3H5 NEfvSpNKSzVyIE2gN{46KM7:TR?= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR4MUiyO{c,Ojl2NkG4Nlc9N2F-
HT-29 MX7BcpRq[2GwY3XyJIFkfGm4aYT5JIF{e2G7 NFz1[nVKSzVyIE2gN{46PyEQvF2= NEHZOIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0PVg1OSd-Mkm1OFk5PDF:L3G+
SF268 MYDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mk\uWGdKKD1iMz65PEDPxE1? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
SNB75 NW[1d2NFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmTRWGdKKD1iMz65PEDPxE1? NHPJW5g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
OVCAR3 M1XXdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWPUS2khRSB|Lkm4JO69VQ>? NXz2e|F4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
SNB19 M{ntdmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnW4WGdKKD1iMz65PEDPxE1? NGT1Wms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
Hs 578T M2rwTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV;US2khRSB|Lkm4JO69VQ>? NH6wfZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
TK10 NFrpWIRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWjHTVUxKD1iMz65PEDPxE1? M1LKO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
BT549 MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV;jdXM2XEeLIE2gN{46QCEQvF2= NFLRW|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
Caki1 MlvXRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NHK2SpRVT0liPTCzMlk5KM7:TR?= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
TK10 NE\kW5lCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= Mn3VS2k2OCB;IEOuPVgh|ryP NFLqd|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
MDA-MB-435 NV;kclBoSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MXjUS2khRSB|Lkm4JO69VQ>? M{DZOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
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HepG2 NYi2UG5oS3m2b4TvfIlkcXS7IHHzd4F6 M2\TbWlEPTBiPTC2MlIh|ryP M1:wZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OE[1Nlc3Lz5{OEi2OVI4PjxxYU6=
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OS-RC2 NV\mTlN[S3m2b4TvfIlkcXS7IHHzd4F6 NYX4O21PUUN3MDC9JFch|ryP MnXQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3MUe5NFgoRjJ7NUG3PVA5RC:jPh?=
OS-RC2 MWLDfZRwfG:6aXPpeJkh[XO|YYm= M1P0dWlEPTBiPTC3JO69VQ>? M1X1U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7Mkm3Olg5Lz5{OUK5O|Y5QDxxYU6=
COLO205 NUnQeXV4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkhcHWvYX6= MnTnTWM2OCB;IEeuNFQh|ryP NF76cFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEK0NlU2Oyd-MkiyOFI2PTN:L3G+
rhabdomyosarcoma NVLTV2x1S3m2b4TvfIlkcXS7IHHjeIl3cXS7IHHnZYlve3RiaIXtZY4> MUPDR|UxKD1iNz6wOUDPxE1? MmHxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd{OEixPFYoRjJ5Mki4NVg3RC:jPh?=
HuH7 NHXicopEgXSxdH;4bYNqfHliYYPzZZk> NUPOWXBYUUN3MDC9JFcvOSEQvF2= MkLJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzNUm0PFMoRjJ4MUW5OFg{RC:jPh?=
MDA-MB-231 MkG0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MYjJR|UxKD1iNz6xPEDPxE1? NVr1Z2tPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3NlE6OjRpPkKyO|IyQTJ2PD;hQi=>
FLT3-negative K562 Ml2xR5l1d3SxeHnjbZR6KGG|c3H5 MkTNS2k2OCB;IEeuN{DPxE1? NWrqN2ZHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NVg4ODlpPkKzOlE5PzB7PD;hQi=>
K562 NEf5ToRHfW6ldHnvckBie3OjeR?= Ml3iS2k2OCB;IEeuN{DPxE1? NXXoSpJCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFExOjNpPkK2NFgyODJ|PD;hQi=>
K562 NY\TVGdzTnWwY4Tpc44h[XO|YYm= Ml7lS2k2OCB;IEeuN{DPxE1? NFTnSlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NVAzOyd-Mk[wPFExOjN:L3G+
MCF7 M4H1e2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NFnncHdKSzVyIE2gO{4{OyEQvF2= M{LneFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkS0NlE6Lz5{M{[0OFIyQTxxYU6=
A549 NVHGc3dDS3m2b4TvfIlkcXS7IHHzd4F6 M1zodnRIUSB;IEeuN|Yh|ryP NUDVZmNMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
WI38 M2DNS2N6fG:2b4jpZ4l1gSCjc4PhfS=> MVvJR|UxKD1iNz61OEDPxE1? MlTBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6Mk[4NVUoRjJ2OEK2PFE2RC:jPh?=
A375 NIn4RYdEgXSxdH;4bYNqfHliYYPzZZk> MWTJR|UxKD1iNz61OkDPxE1? Mn7PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MUW4NVgoRjJ2MkG1PFE5RC:jPh?=
MDA-MB-231 MnvTR5l1d3SxeHnjbZR6KGG|c3H5 NWX2UIpLUUN3MDC9JFcvPjJizszN MkG4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzOEG0NVQoRjJyMUixOFE1RC:jPh?=
786-0 M2ruemN6fG:2b4jpZ4l1gSCjc4PhfS=> NImyU|JVT0liPTC3MlYzKM7:TR?= NUTZW2VFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
HS27 NIrJXlNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NFm2Z|VKSzVyIE2gO{45KM7:TR?= MmnLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6NUe5OlMoRjF7OEW3PVY{RC:jPh?=
HS27 MkjvR5l1d3SxeHnjbZR6KGG|c3H5 MYjJR|UxKD1iNz64JO69VQ>? NV\JbHpERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4PVc{PjZpPkG5PFk4OzZ4PD;hQi=>
HCT116 NV;3SZI3S3m2b4TvfIlkcXS7IHHzd4F6 NVTVTYp{UUN3MDC9JFcvQCEQvF2= M4mwcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEizOVkzLz5{MkS4N|U6OjxxYU6=
HS27 MlPORY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MlXxTWM2OCB;IEeuPFUh|ryP MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR4NEi4O{c,OTl2NkS4PFc9N2F-
HS27 NVvYVFdiSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MUTHTVUxKD1iNz64OUDPxE1? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN3M{W3NUc,OjF|NUO1O|E9N2F-
A549/ATCC MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVjaNIRtXEeLIE2gO{46PCEQvF2= NHfZU|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
DU145 MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MV\US2khRSB5Lkm0JO69VQ>? MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
NCI/ADR-RES NFj6fXVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHfrNo1VT0liPTC3Mlk1KM7:TR?= NH\Qd4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
MCF7 MmD1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3vpW3RIUSB;IEeuPVQh|ryP NHq5O2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
PC3 MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M3X5[XRIUSB;IEeuPVQh|ryP M4nPUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
UO31 M{n4[mFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NFziN4tVT0liPTC3Mlk1KM7:TR?= NGi0R4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
NCI-ADR-RES MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NYTEfog4XEeLIE2gO{46PCEQvF2= NWrlZ2xzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
LOXIMVI MmnCRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NHjCPJRNSzVyIE2gO{46PCEQvF2= NGTsboY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
SK-MEL-28 M4TaS2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MoTWWGdKKD1iNz65OEDPxE1? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
KM12 MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M3XKcnRIUSB;IEeuPVQh|ryP NFLMc3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
COLO205 NI\XNGlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NF;4Wm5VT0liPTC3Mlk1KM7:TR?= MnfoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
HT-29 NFzFW3dCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MYfUS2khRSB5Lkm0JO69VQ>? MnTRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
NCI-H226 M4XYfmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MlrsWGdKKD1iNz65OEDPxE1? MkPCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
HOP62 M3rEfmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M3rFbHRIUSB;IEeuPVQh|ryP M4L6fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
T47D M2\pVmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MWXUS2khRSB5Lkm0JO69VQ>? M3fF[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
MDA-MB-468 M3r2OWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MmXPWGdKKD1iNz65OEDPxE1? NF;mNJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
EKVX M4HtWWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MVjUS2khRSB5Lkm0JO69VQ>? NYm3OJdrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
MCF7 MW\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M2DmVXRIUSB;IEeuPVQh|ryP NYDsVJVCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
Hs 578T MWfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M3PT[nRIUSB;IEeuPVQh|ryP MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
MGC803 NFXEc|lCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M1fMWmlEPTBiPTC3Mlk6KM7:TR?= NEnhXnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW2NFA1QSd-Mk[1OlAxPDl:L3G+
HCT116 MXHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M4XydGlEPTBiPTC4MlA5KM7:TR?= M4\zXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NESwOFc6Lz5{NES0NFQ4QTxxYU6=
PC3 M3;TXGN6fG:2b4jpZ4l1gSCjc4PhfS=> MXfJR|UxKD1iOD6wPEDPxE1? M{XsTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MESzNlY5Lz5{N{C0N|I3QDxxYU6=
HCT116 NEPlVJpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NUT4[4tJUUN3MDC9JFgvODhizszN M3TOWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkS0NlE6Lz5{M{[0OFIyQTxxYU6=
WM266.4 NYj6OnJOSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= Mn7sS2k2OCB;IEiuNUDPxE1? NXX1bo04RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNlU6QTZpPkKzNFI2QTl4PD;hQi=>
WM266.4 NXL6UWdISW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MYrHTVUxKD1iOD6xNkDPxE1? MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV6M{[2PUc,OjJ3OEO2Olk9N2F-
Ketr3 M2LiOGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M3j0VGlEPTBiPTC4MlI4KM7:TR?= MmjNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{MU[4OFkoRjNyMkG2PFQ6RC:jPh?=
A375 M4CzSGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MoLaTWM2OCB;IEiuNlch|ryP NIPwfFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEKxOlg1QSd-M{CyNVY5PDl:L3G+
HepG2 MUfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NY\sb2MxUUN3MDC9JFgvOjdizszN M4DKUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkG2PFQ6Lz5|MEKxOlg1QTxxYU6=
MX1 M4DZd2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NXPjc4hiUUN3MDC9JFgvOjdizszN MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJzNki0PUc,OzB{MU[4OFk9N2F-
MX1 MmHIR5l1d3SxeHnjbZR6KGG|c3H5 Mn3MTWM2OCB;IEiuNlch|ryP NU\I[FRTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2O|UyOzVpPkK0Olc2OTN3PD;hQi=>
WM266.4 NHPmWIhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkKwS2k2OCB;IEiuN{DPxE1? NHXlW5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkO2NVY5Pid-MkKzOlE3QDZ:L3G+
PLC/PRF/5 MXPDfZRwfG:6aXPpeJkh[XO|YYm= NEizcpVKSzVyIE2gPE4{KM7:TR?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTV|MUC1N{c,OjF3M{GwOVM9N2F-
A375 M3PWWWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M4LpUGlEPTBiPTC4MlM{KM7:TR?= NYrZO|B1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm4PFY{OjRpPkK5PFg3OzJ2PD;hQi=>
HCT116 M4fVNWN6fG:2b4jpZ4l1gSCjc4PhfS=> Mnv6TWM2OCB;IEiuOFEh|ryP NITLR4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[zNVc5QCd-Mkm2N|E4QDh:L3G+
WI38 NEDKbmJEgXSxdH;4bYNqfHliYYPzZZk> NYG2R|FoUUN3MDC9JFgvPDJizszN MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjl7MUmzPEc,OjZ7OUG5N|g9N2F-
HepG2 NH;wPItEgXSxdH;4bYNqfHliYYPzZZk> MlTDTWM2OCB;IEiuOFIh|ryP NHfMR2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G2NlEzOyd-MkexOlIyOjN:L3G+
HepG2 NIO1TXFEgXSxdH;4bYNqfHliYYPzZZk> Mn;hTWM2OCB;IEiuOFIh|ryP MlzrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6N{G5NFkoRjJ|OEexPVA6RC:jPh?=
HT-29 MkW3RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M4rBUmlEPTBiPTC4MlQ1KM7:TR?= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZyMk[3OEc,Ojl4MEK2O|Q9N2F-
HepG2 MmG4R5l1d3SxeHnjbZR6KGG|c3H5 M{X6cGlEPTBiPTC4MlY4KM7:TR?= NECx[Hg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[3OVE{PSd-MkS2O|UyOzV:L3G+
MCF7 MljZR5l1d3SxeHnjbZR6KGG|c3H5 NXf1TGJ5UUN3MDC9JFgvQDNizszN MmDEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2M{W0O|koRjJyNEO1OFc6RC:jPh?=
MCF7 NVuwS|VpS3m2b4TvfIlkcXS7IHHzd4F6 NFHIeY5KSzVyIE2gPU4yOiEQvF2= NGW4NmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOwNFkzOCd-MkSzNFA6OjB:L3G+
HepG2 MYLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M3PiOWlEPTBiPTC5MlE1KM7:TR?= NX;Bb49pRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1OlAxPDlpPkK2OVYxODR7PD;hQi=>
A375 Mo\rR5l1d3SxeHnjbZR6KGG|c3H5 NEfuWoxKSzVyIE2gPU4yPyEQvF2= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ5NUGzOUc,OjR4N{WxN|U9N2F-
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SKOV3 NW\Kdmo4S3m2b4TvfIlkcXS7IHHzd4F6 MkX1TWM2OCB;IEmuNlUh|ryP NXrFNotoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNFA6OjBpPkK0N|AxQTJyPD;hQi=>
RS4:11 MUHGeY5kfGmxbjDhd5NigQ>? M{jOSGdKPTBiPTC5MlMh|ryP M{LvfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEixNFI{Lz5{NkC4NVAzOzxxYU6=
RS4:11 MoPCR5l1d3SxeHnjbZR6KGG|c3H5 MUTHTVUxKD1iOT6zJO69VQ>? NVvkWXdnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NVg4ODlpPkKzOlE5PzB7PD;hQi=>
MIAPaCa2 M{XvPGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MX;JR|UxKD1iOT6zNkDPxE1? NHvtWoc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUCzNlA{OSd-MkmwN|IxOzF:L3G+
PANC1 Ml3jS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGnWW3JKSzVyIE2gPU4{OiEQvF2= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTFyMkG3OUc,OjlzMEKxO|U9N2F-
MHCC97H MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4fZZ2lEPTBiPTC5MlMzKM7:TR?= M1LBO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUCyNVc2Lz5{OUGwNlE4PTxxYU6=
RS4:11 NUPMNmxkSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M4qx[mdKPTBiPTC5MlQh|ryP NITq[G49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUewPFQ3QCd-MkG3NFg1Pjh:L3G+
Hep3B NYPjWVBHS3m2b4TvfIlkcXS7IHHzd4F6 NHjMS4NKSzVyIE2gPU41KM7:TR?= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh4NUK3Okc,Ojh6NkWyO|Y9N2F-
LoVo MmfjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWnJR|UxKD1iOT60O{DPxE1? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTFyMkG3OUc,OjlzMEKxO|U9N2F-
SMMC7721 NF:5SmNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NWr1W4pGUUN3MDC9JFkvQTZizszN Mnu3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4M{exNlMoRjJ3NkO3NVI{RC:jPh?=
MCF7 MVvDfZRwfG:6aXPpeJkh[XO|YYm= NGeyZopVT0liPTC5Mlk4KM7:TR?= NU\OdXB2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
IGROV1 MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUHUS2khRSBzMDFOwG0> NWjWPIhWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
ACHN M1HDdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3HET3RIUSB;IEGwJO69VQ>? MoThQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
TK10 M1LsW2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml:2WGdKKD1iMUCg{txO NGfnZ409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
786-0 Mli2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M2n3dnRIUSB;IEGwJO69VQ>? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
SN12C M{\ab2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MVzUS2khRSBzMDFOwG0> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
Caki1 MnzmRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M2fiZmxEPTBiPTCxNEDPxE1? M4rTU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
TK10 M2HwemFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NH\URlJVT0liPTCxNEDPxE1? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
RXF393 M2XEcWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MojBWGdKKD1iMUCg{txO NFvme3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
OVCAR8 NHzDc|hCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NVLnZW9xXEeLIE2gNVAh|ryP M3fhdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
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MDA-MB-435 NVK5V|FoSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MXvMR|UxKD1iMUCg{txO NGPLfJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
SF539 MljnRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M1roNXRIUSB;IEGwJO69VQ>? NX;DZ4JGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
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DU145 MY\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M3zYSHRIUSB;IEGwJO69VQ>? NGK3Z2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
K1 M4[2PGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MkXqTWM2OCB;IEGwMlIh|ryP NVezU2M3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmwN|IxOzFpPkK5NFMzODNzPD;hQi=>
Bel7402 NWnSVJR1S3m2b4TvfIlkcXS7IHHzd4F6 NXnJPXNWUUN3MDC9JFExNjJ4IN88US=> M4Sx[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUC0NlA1Lz5{MUWwOFIxPDxxYU6=
Bel7402 NIjTOIFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml;tTWM2OCB;IEGwMlMyKM7:TR?= NFOxRWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUGwNlE4PSd-MkmxNFIyPzV:L3G+
OVCAR3 M3racmN6fG:2b4jpZ4l1gSCjc4PhfS=> NXTtW|lyXEeLIE2gNVAvOzJizszN NFKwdHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
SGC7901 MkPuR5l1d3SxeHnjbZR6KGG|c3H5 M2rvbmlEPTBiPTCxNE4{PyEQvF2= M1XxelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEO1OFc6Lz5{MESzOVQ4QTxxYU6=
SNB75 M4PKSGN6fG:2b4jpZ4l1gSCjc4PhfS=> M2DlR3RIUSB;IEGwMlQzKM7:TR?= M3;ET|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmwOVA5Lz5{NkW5NFUxQDxxYU6=
A431 NYLReHBWSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MX7JR|UxKD1iMUCuOFYh|ryP NYD4dHBoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmwN|IxOzFpPkK5NFMzODNzPD;hQi=>
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GES-1 MUDDfZRwfG:6aXPpeJkh[XO|YYm= M17Mb2lEPTBiPTCxNE43QCEQvF2= NF:zU2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G2NlEzOyd-MkexOlIyOjN:L3G+
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Hep3B MnL4R5l1d3SxeHnjbZR6KGG|c3H5 M{DBNGlEPTBiPTCxNU4zKM7:TR?= NH7sZ5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEO0NlQxOCd-MkizOFI1ODB:L3G+
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MCF7 Mkj1R5l1d3SxeHnjbZR6KGG|c3H5 NUL4fFNjUUN3MDC9JFEyNjN2IN88US=> M4rBNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEexPVA6Lz5{M{i3NVkxQTxxYU6=
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HepG2 M323SGN6fG:2b4jpZ4l1gSCjc4PhfS=> MYjJR|UxKD1iMUGuOFkh|ryP M363XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUC0NlA1Lz5{MUWwOFIxPDxxYU6=
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SGC7901 MnPPR5l1d3SxeHnjbZR6KGG|c3H5 M4f1ZWlEPTBiPTCxNU42KM7:TR?= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDNyMEmyNEc,OjR|MEC5NlA9N2F-
HeLa NUntNZVWSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= Mnz2TWM2OCB;IEGyMlAyKM7:TR?= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTFyM{i3N{c,OjlzMEO4O|M9N2F-
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HepG2 MlHJRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MVzJR|UxKD1iMUKuOVQh|ryP MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB|MkCzNUc,OjlyM{KwN|E9N2F-
A549 NFHudGZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MXzJR|UxKD1iMUKuOVQh|ryP M{TvTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkS0NlE6Lz5{M{[0OFIyQTxxYU6=
Caki1 MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mmn2WGdKKD1iMUKuOVkh|ryP MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
OVCAR8 NGnJW21Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkHMWGdKKD1iMUKuOVkh|ryP MoDrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
SKOV3 MlztRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M3TiTXRIUSB;IEGyMlYh|ryP M4fhNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
ACHN MmTERY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M4HMVnRIUSB;IEGyMlYh|ryP MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
OVCAR3 MlLxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MXfUS2khRSBzMj62JO69VQ>? MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
OVCAR4 NXG4clI4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M4XqVXRIUSB;IEGyMlYh|ryP MnKwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
HCT15 MlfvRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MUnUS2khRSBzMj62JO69VQ>? MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
SW620 MVPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M2nDUHRIUSB;IEGyMlYh|ryP NF33O5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
A549/ATCC M{nOfmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MYLUS2khRSBzMj62JO69VQ>? MmjDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
MDA-MB-468 NHi4WnpEgXSxdH;4bYNqfHliYYPzZZk> MYnUS2khRSBzMz6wPUDPxE1? NXziWJVyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
SK-MEL-28 M3H1VGN6fG:2b4jpZ4l1gSCjc4PhfS=> MXrUS2khRSBzMz6zPUDPxE1? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
A549 NIXOXFlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= Ml;ETWM2OCB;IEGzMlY1KM7:TR?= MlzXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3NkCwOFkoRjJ4NU[wNFQ6RC:jPh?=
A375 M13XO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIh2dWGw MUPJR|UxKD1iMUOuOlQh|ryP MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ2MkW1N{c,Ojh{NEK1OVM9N2F-
A375 MmfwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NIfM[YdKSzVyIE2gNVMvPjRizszN MknKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NkKyOlcoRjJ3NE[yNlY4RC:jPh?=
K562 MnjwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NHS4WHdKSzVyIE2gNVMvQDVizszN MnvQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NESyNVkoRjJ|NkS0NlE6RC:jPh?=
Hep3B M2\ZWGN6fG:2b4jpZ4l1gSCjc4PhfS=> M{Hkc2lEPTBiPTCxOE4xQCEQvF2= M{e4UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUC0NlA1Lz5{MUWwOFIxPDxxYU6=
T47D NHnxVoNEgXSxdH;4bYNqfHliYYPzZZk> MWLUS2khRSBzND6zPEDPxE1? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
NCI-H226 MYfDfZRwfG:6aXPpeJkh[XO|YYm= NEXJc4JVT0liPTCxOE41OiEQvF2= M{i2fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmwOVA5Lz5{NkW5NFUxQDxxYU6=
MDA-MB-231 M2rrd2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NUfRWIJMUUN3MDC9JFE1NjZ{IN88US=> MoK2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NEC0O|koRjJ2NESwOFc6RC:jPh?=
MDA-MB-231 NIPYUo9CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= Mn\uTWM2OCB;IEG0MlYzKM7:TR?= MkHhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NESyNVkoRjJ|NkS0NlE6RC:jPh?=
NCI-H522 NEe3ZWlEgXSxdH;4bYNqfHliYYPzZZk> NW\GXmh6XEeLIE2gNVQvQDJizszN MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
SK-MEL-2 NWXjUphCS3m2b4TvfIlkcXS7IHHzd4F6 M2rRZXRIUSB;IEG0Mlg2KM7:TR?= Mn31QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
OS-RC2 M{n4emN6fG:2b4jpZ4l1gSCjc4PhfS=> M2j4O2lEPTBiPTCxOUDPxE1? M3O1flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{i2OFM3Lz5{OUe4OlQ{PjxxYU6=
HepG2 MV3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NYHBfI1IUUN3MDC9JFE2KM7:TR?= NX3Ddld[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxNFM5PzNpPkK5NVA{QDd|PD;hQi=>
fibroblast MnPZRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M3TVbGlEPTBiPTCxOU4yKM7:TR?= NFfjWHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOES3O|UxPSd-MUi0O|c2ODV:L3G+
HT-29 MmnhR5l1d3SxeHnjbZR6KGG|c3H5 NVHDdW1KUUN3MDC9JFE2NjJizszN NYL5WHVkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2O|UyOzVpPkK0Olc2OTN3PD;hQi=>
U87MG NYizdXQ6S3m2b4TvfIlkcXS7IHHzd4F6 M3fO[mlEPTBiPTCxOU42PyEQvF2= Moq2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6Mk[4NVUoRjJ2OEK2PFE2RC:jPh?=
BT549 NIS3UWVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M3PmVWxEPTBiPTCxOU45KM7:TR?= NYnSVZB[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
GES-1 Mn20R5l1d3SxeHnjbZR6KGG|c3H5 M3fLV2lEPTBiPTCxOU45KM7:TR?= NIDRV4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i3NVkxQSd-MkO4O|E6ODl:L3G+
SNB19 NXjiTYxRSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NXOyNG52XEeLIE2gNVUvQDVizszN MnTtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
IGROV1 NY\LPItNSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NIj0T2JVT0liPTCxOU46KM7:TR?= NYX0Z4lvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
HCT116 MX7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NX3PNIdxVEN3MDC9JFE2NjlizszN MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
OS-RC2 M{Did2N6fG:2b4jpZ4l1gSCjc4PhfS=> M1ruRWlEPTBiPTCxOkDPxE1? MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR3NECxO{c,OjN2NUSwNVc9N2F-
A375 NGPuV|dEgXSxdH;4bYNqfHliYYPzZZk> NUDyZohQUUN3MDC9JFE3NjJ2IN88US=> NVq0Zlh5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OlE{OThpPkK1OFYyOzF6PD;hQi=>
SKOV3 MnrYR5l1d3SxeHnjbZR6KGG|c3H5 M2D6Z3RIUSB;IEG2MlM3KM7:TR?= M2DvWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmwOVA5Lz5{NkW5NFUxQDxxYU6=
WM1361 NF;yV5FCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MVXJR|UxKD1iMU[uOFQh|ryP NYrsNnlSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2NFI3PzRpPkK5OlAzPjd2PD;hQi=>
MKN28 MYXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? Mm\ETWM2OCB;IEG3JO69VQ>? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB|MkCzNUc,OjlyM{KwN|E9N2F-
SMMC7721 NYDsUXhoS3m2b4TvfIlkcXS7IHHzd4F6 M4G4OmlEPTBiPTCxO{4{KM7:TR?= MkP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MESyNFQoRjJzNUC0NlA1RC:jPh?=
UACC257 M3myV2N6fG:2b4jpZ4l1gSCjc4PhfS=> NIP4dmlVT0liPTCxO{4{OyEQvF2= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
EAhy926 MoLqRY51cWGwZ3nv[4VvcWNiYXP0bZZqfHliYYPzZZk> NVnuZ4U6UUN3MDC9JFE5NjV{IN88US=> MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB|MkCzNUc,OjlyM{KwN|E9N2F-
SMMC7721 MmfWR5l1d3SxeHnjbZR6KGG|c3H5 MV7JR|UxKD1iMUiuO{DPxE1? M37vZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{CwPVIxLz5{NEOwNFkzODxxYU6=
Ketr3 Ml7FR5l1d3SxeHnjbZR6KGG|c3H5 NF;BPVRKSzVyIE2gNVgvQCEQvF2= NW[1XWFwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2O|UyOzVpPkK0Olc2OTN3PD;hQi=>
NCI-H522 NH\VbGREgXSxdH;4bYNqfHliYYPzZZk> M{XBcWlEPTBiPTCxPU4zPiEQvF2= M1;pU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NE[xN|E5Lz5{NUS2NVMyQDxxYU6=
A549 M3HwR2N6fG:2b4jpZ4l1gSCjc4PhfS=> Mmn3TWM2OCB;IEG5MlU1KM7:TR?= NXLvNFNlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexOlIyOjNpPkK3NVYzOTJ|PD;hQi=>
SH-SY5Y NVHoTnRTS3m2b4TvfIlkcXS7IHHzd4F6 MXzJR|UxKD1iMUmuOVQh|ryP M1vNclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEexPVA6Lz5{M{i3NVkxQTxxYU6=
MGC803 MoDURY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M1[1WmlEPTBiPTCxPU46OiEQvF2= NYG0[25sRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxNFM5PzNpPkK5NVA{QDd|PD;hQi=>
EKVX NWDZTW5mT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3u1XnRIUSB;IEG5Mlk2KM7:TR?= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
OVCAR5 M2fzNmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MkTFWGdKKD1iMkCg{txO NXr0WJE3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
UACC62 NV3YTYIxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NWHsO2VvVEN3MDC9JFIxKM7:TR?= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
UACC257 NHLpfYlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M4eyc2xEPTBiPTCyNEDPxE1? M1S2NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
MDA-MB-231 M{DyOGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M2\NeGxEPTBiPTCyNEDPxE1? M2PtTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
U87 M4nIO2N6fG:2b4jpZ4l1gSCjc4PhfS=> M13NbGlEPTBiPTCyNU4xPyEQvF2= NELkS4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESzOVQ4QSd-MkC0N|U1Pzl:L3G+
EJ MlzHR5l1d3SxeHnjbZR6KGG|c3H5 NX\DXVJuUUN3MDC9JFIzNjlizszN MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDNyMEmyNEc,OjR|MEC5NlA9N2F-
HCT116 MnfGRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MnzqTWM2OCB;IEKzMlMyKM7:TR?= M2q3c|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkCyOlc1Lz5{OU[wNlY4PDxxYU6=
HOP62 Mn;OR5l1d3SxeHnjbZR6KGG|c3H5 NHe5O|RVT0liPTCyN{42PSEQvF2= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
MDA-MB-231 NFf6bI1EgXSxdH;4bYNqfHliYYPzZZk> MljZWGdKKD1iMkOuPVMh|ryP M4[2SVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmwOVA5Lz5{NkW5NFUxQDxxYU6=
PC3 MXnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NHThb5JKSzVyIE2gNlQvOiEQvF2= MmPUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NEC0O|koRjJ2NESwOFc6RC:jPh?=
PC3 NHLLUnpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= Mnj5TWM2OCB;IEK0MlIh|ryP MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ2NEKxPUc,OjN4NESyNVk9N2F-
EAhy926 NXfRXmFxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MV3JR|UxKD1iMkSuN|Yh|ryP MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB4OEW5PUc,OjhyNki1PVk9N2F-
EAhy926 MoLoRY51cS2jbnfpc4dmdmmlIHHjeIl3cXS7IHnuJIh2dWGw NH22RotKSzVyIE2gNlQvOzZizszN MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTFyMkG3OUc,OjlzMEKxO|U9N2F-
U251 MXzDfZRwfG:6aXPpeJkh[XO|YYm= MWfJR|UxKD1iMkSuO|Eh|ryP M3:3W|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEO1OFc6Lz5{MESzOVQ4QTxxYU6=
NIH/3T3 NFPocmRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NEDhc3pKSzVyIE2gNlQvPzVizszN NXrtOXhORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1PVI3OjhpPkKxOVkzPjJ6PD;hQi=>
NIH/3T3 M3jD[2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MUXJR|UxKD1iMkSuO|Uh|ryP MknKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEe3NlAoRjJ{NkS3O|IxRC:jPh?=
DU145 M1TsPWN6fG:2b4jpZ4l1gSCjc4PhfS=> NXvGUIU{UUN3MDC9JFI1NjlzIN88US=> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF4MkGyN{c,OjdzNkKxNlM9N2F-
DU145 M3Tu[2N6fG:2b4jpZ4l1gSCjc4PhfS=> NFvZRVhKSzVyIE2gNlQvQTFizszN MnztQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6N{G5NFkoRjJ|OEexPVA6RC:jPh?=
U251 MmnZRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NXG5V3h1VEN3MDC9JFI2NjFizszN Ml7IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
M14 NIDnNFFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NF7P[FhNSzVyIE2gNlUvOSEQvF2= M3HBZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
NCI-H522 NY\QOJA6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MmCwUGM2OCB;IEK1MlEh|ryP M1nkcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
OVCAR4 MofnS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoTOWGdKKD1iMkWuNVIh|ryP Mlz2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
SH-SY5Y MXfDfZRwfG:6aXPpeJkh[XO|YYm= NXPrSIduUUN3MDC9JFI4NjdzIN88US=> MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF4MkGyN{c,OjdzNkKxNlM9N2F-
A549 NEjWXplEgXSxdH;4bYNqfHliYYPzZZk> M17HWmlEPTBiPTCyO{44OSEQvF2= MmHHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6N{G5NFkoRjJ|OEexPVA6RC:jPh?=
PLC/PRF/5 NUHUfGtIS3m2b4TvfIlkcXS7IHHzd4F6 NXXXVmN3UUN3MDC9JFI6NjlizszN Mn6wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzMEm5OFgoRjJ6MUC5PVQ5RC:jPh?=
SK-MEL-5 NGW5PVdEgXSxdH;4bYNqfHliYYPzZZk> M2W1UHRIUSB;IEOxMlI3KM7:TR?= Ml3SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
A498 NXjMVVJwSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NIq5fJRNSzVyIE2gN|EvPiEQvF2= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
SK-MEL-28 Mni2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NVLWTHNGVEN3MDC9JFMyNjZizszN NVTPfHVtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
KM12 MW\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M1e5O2xEPTBiPTCzNU43KM7:TR?= Mof3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
COLO205 MknsRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NYP6fXF1VEN3MDC9JFMyNjZizszN MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
NCI-H226 NEP4SXJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MlnTUGM2OCB;IEOxMlYh|ryP NYHQOoVPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
SF295 NWPDblc6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NXzJcpB[VEN3MDC9JFMyNjZizszN MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
HOP62 MVLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MnvaUGM2OCB;IEOxMlYh|ryP NHrpTW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
T47D M1vRRmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NGPYZmZNSzVyIE2gN|EvPiEQvF2= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
MHCC97L M2rKemN6fG:2b4jpZ4l1gSCjc4PhfS=> NGPHPHFKSzVyIE2gN|QvPDFizszN MnHPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzMEm5OFgoRjJ6MUC5PVQ5RC:jPh?=
MDA-MB-231 NXfE[VY4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NI[xeFZKSzVyIE2gN|Uh|ryP MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDNzNUG5Nkc,OjR|MUWxPVI9N2F-
MDA-MB-231 NH7vN5JEgXSxdH;4bYNqfHliYYPzZZk> M3LIUmlEPTBiPTCzOkDPxE1? NITo[Wc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS4N|U6Oid-MkK0PFM2QTJ:L3G+
B16-BL6 NF7ncoZEgXSxdH;4bYNqfHliYYPzZZk> M4q3eWlEPTBiPTCzOk41QCEQvF2= NHmxO2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[zNVc5QCd-Mkm2N|E4QDh:L3G+
UO31 MoTrRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M1OwTmxEPTBiPTCzPU45KM7:TR?= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
SN12C NY\RfGpZSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= Mk\LUGM2OCB;IEO5Mlgh|ryP NXf2[lhFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
RXF393 MX7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NGnxOnVNSzVyIE2gN|kvQCEQvF2= NFfDOI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
SNB75 MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NXjOUlJNVEN3MDC9JFM6NjhizszN M4Ljd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
SF268 MVfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NIfRU21NSzVyIE2gN|kvQCEQvF2= MnjwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
NCI-H23 NXvXdGE1SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MXrMR|UxKD1iM{muPEDPxE1? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
HT-29 M4rYT2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M4DBUGxEPTBiPTCzPU45KM7:TR?= NYjaZpNrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
HOP92 MUfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NWnlVItLVEN3MDC9JFM6NjhizszN NYrZPWZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
NCI-H460 M4G5VGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MlnIUGM2OCB;IEO5Mlgh|ryP NYP3eW17RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
SW620 NH;5cW1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MWXMR|UxKD1iM{muPEDPxE1? NHfUN5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
MDA-MB-468 NGLOVIZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M4P3R2xEPTBiPTCzPU45KM7:TR?= NWrUV5o1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
MALME-3M NICxRpZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnPKWGdKKD1iM{muPFEh|ryP MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
LC3-I / LC-3II / ATG5; 

PubMed: 23392173     


Sorafenib induces the conversion of LC3 in a dose-dependent manner. PLC5, Hep3B, SK-Hep1 and HepG2 were exposed to sorafenib at the indicated doses for 16 h and the expression levels of LC3-II were analyzed by western blot.

p-STAT3 / STAT3 / Mcl-1; 

PubMed: 23392173     


Effects of sorafenib on STAT3-related proteins in HCC cells. The cells were treated with sorafenib at the indicated dose for 16 h. 

β-catenin / Survivin / Mcl-1 / PTMA; 

PubMed: 26517516     


Co-inhibition of β-catenin and PTMA by sorafenib in HCC cells. Cell lines indicated on top were treated or not with 10 μM sorafenib for 24 hrs and processed for immuno-blotting. IC50 values (the concentration of sorafenib that inhibits 50% of cell growth) for each cell line are indicated below the panels.

pERK / ERK; 

PubMed: 22286758     


Western blot analysis of p-ERK (T202/Y204) and ERK at indicated time points in HCT116 cells treated with 20 μmol/L sorafenib.

p-PKM2(y105) / PMK2 / Caspase-9; 

PubMed: 26959741     


Sorafenib downregulates the p-PKM2 (Y105) at the indicated doses after treatment for 24 h.

RET(pY1016) / VEGFR2(pY1214) / MEK1(pT292) / ERK(pY204); 

PubMed: 22941289     


Sorafenib affected the phosphorylation of receptor tyrosine kinase RET and VEGFR2, as well as MEK/ERK kinases signaling cascades in three cell lines. Three cell lines were treated by sorafenib with two concentration gradients, 1 and 5 μmol/L/L, and then collected after 2, 4, and 8 h, cells without sorafenib treatment were as the controls (0 h). Total proteins were extracted and quantified to be used in Western blot assays. (A) Sorafenib inhibited RET and VEGFR2 phosphorylation dose-dependently while activated MEK and ERK phosphorylation in A549 cells. (B) Sorafenib also inhibited RET and VEGFR2 phosphorylation, and slightly activated MEK and ERK phosphorylation in HeLa cells. (C) Sorafenib activated the phosphorylation of RET, VEGFR2, and MEK, but inhibited ERK phosphorylation in HepG2 cells.

Cyclin D1; 

PubMed: 26039995     


Dose-escalation effects of sorafenib or SC-1 for 24 h on STAT3-related proteins in HSC-T6 and LX2 cells.

23392173 26517516 22286758 26959741 22941289 26039995
Growth inhibition assay
Cell viability; 

PubMed: 26039995     


Dose-escalation and time-dependent effects of sorafenib for 24 or 48 h on cell viability in HSC-T6, LX2, and mouse primary HSCs. Circles, mean; bars, SE (n = 3).

26039995
Immunofluorescence
p65; 

PubMed: 22286758     


HCT116 cells were treated with 20 μmol/L sorafenib or 10 ng/mL TNF-α for 3 hours then fixed. Immunofluorescence was carried out as described in the Materials and Methods for p65 (green) and DAPI (blue). Representative pictures (400×) are shown.

cytochrome c; 

PubMed: 22278289     


Immunoflourescent staining and quantification of mitochondrial membrane potential (appearing in red, mitotracker) and cytochrome c (appearing in green, FITC) in 22Rv1 and PC3 treated with 20 μM sorafenib for 24 h.

22286758 22278289
ELISA
TGF-beta / CD206; 

PubMed: 26158762     


In Macrophage, TGF-β secretion and CD206 were confirmed by ELISA.

Caspase-9 / Caspase-3; 

PubMed: 30923462     


Caspase-9 and caspase-3 activities were measured via ELISA assay. FCCP, an activator of mitophagy, was added into the medium of sorafenib-treated cells to activate mitophagy. Adenovirus-loaded LATS2 (Ad-LATS2) was transfected into HepG2 cells in the presence of sorafenib. *p < 0.05 vs. control group; #p < 0.05 vs. Sorafenib + Ad-cont group; #p < 0.05 vs. Sorafenib + Ad-LATS2 group.

26158762 30923462
In vivo Oral administration of Sorafenib (~60 mg/kg) demonstrates broad spectrum, dose-dependent anti-tumor activity against a variety of human tumor xenograft models including MDA-MB-231, Colo-205, HT-29, DLD-1, NCI-H460, and A549, with no evidence of toxicity. In association with the anti-tumor efficacy, Sorafenib treatment potently inhibits MEK 1/2 phosphorylation and pERK 1/2 levels in HT-29 and MDA-MB-231 xenografts but not in Colo-205 xenografts, and significantly suppresses tumor microvessel area (MVA) and microvessel density (MVD) in MDA MB-231, HT-29 and Colo-205 tumor xenografts. [1] Sorafenib treatment produces dose-dependent growth inhibition of PLC/PRF/5 tumor xenografts in SCID mice with TGIs of 49% and 78% at 10 mg/kg and 30 mg/kg, respectively, consistent with the inhibition of ERK and eIF4E phosphorylation, reduction of the microvessel area, and induction of tumor cell apoptosis. [2] Sorafenib sensitizes bax-/- cells to TRAIL in a dose-dependent manner, through a mechanism involving down-regulating NF-κB mediated Mcl-1 and cIAP2 expression. Combining Sorafenib (30-60 mg/kg) with TRAIL (5 mg/kg) show dramatic efficacy in TRAIL-resistant HCT116 bax-/- and HT29 tumor xenografts. [3]

Protocol

Kinase Assay:

[1]

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Biochemical assays:

Recombinant baculoviruses expressing Raf-1 (residues 305–648) and B-Raf (residues 409–765) are purified as fusion proteins. Full-length human MEK-1 is generated by PCR and purified as a fusion protein from Escherichia coli lysates. Sorafenib tosylate is added to a mixture of Raf-1 (80 ng), or B-Raf (80 ng) with MEK-1 (1 μg) in assay buffer [20 mM Tris (pH 8.2), 100 mM NaCl, 5 mM MgCl2, and 0.15% β-mercaptoethanol] at a final concentration of 1% DMSO. The Raf kinase assay (final volume of 50 μL) is initiated by adding 25 μL of 10 μM γ[33P]ATP (400 Ci/mol) and incubated at 32 °C for 25 minutes. Phosphorylated MEK-1 is harvested by filtration onto a phosphocellulose mat, and 1% phosphoric acid is used to wash away unbound radioactivity. After drying by microwave heating, a β-plate counter is used to quantify filter-bound radioactivity. Human VEGFR2 (KDR) kinase domain is expressed and purified from Sf9 lysates. Time-resolved fluorescence energy transfer assays for VEGFR2 are performed in 96-well opaque plates in the time-resolved fluorescence energy transfer format. Final reaction conditions are as follows: 1 to 10 μM ATP, 25 nM poly GT-biotin, 2 nM Europium-labeled phospho (p)-Tyr antibody (PY20), 10 nM APC, 1 to 7 nM cytoplasmic kinase domain in final concentrations of 1% DMSO, 50 mM HEPES (pH 7.5), 10 mM MgCl2, 0.1 mM EDTA, 0.015% Brij-35, 0.1 mg/mL BSA, and 0.1% β-mercaptoethanol. Reaction volumes are 100 μL and are initiated by addition of enzyme. Plates are read at both 615 and 665 nM on a Perkin-Elmer VictorV Multilabel counter at ~1.5 to 2.0 hours after reaction initiation. Signal is calculated as a ratio: (665 nm/615 nM) × 10,000 for each well. For IC50 generation, Sorafenib tosylate is added before the enzyme initiation. A 50-fold stock plate is made with Sorafenib tosylate serially diluted 1:3 in a 50% DMSO/50% distilled water solution. Final Sorafenib tosylate concentrations range from 10 μM to 4.56 nM in 1% DMSO.
Cell Research:

[1]

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  • Cell lines: MDA-MB-231, and HAoSMC
  • Concentrations: Dissolved in DMSO, final concentrations ~10 μM
  • Incubation Time: 72 hours
  • Method:

    Cells are exposed to increasing concentrations of Sorafenib tosylate for 72 hours. Cell number is quantitated using the Cell TiterGlo ATP Luminescent assay kit. This assay measures the number of viable cells per well by measurement of luminescent signal based on amount of cellular ATP.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Female NCr-nu/nu mice implanted s.c. with MDA-MB-231, Colo-205, HT-29, H460, or A549 cells
  • Dosages: ~60 mg/kg
  • Administration: Orally once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 63 mg/mL warmed (135.53 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5%DMSO+45%PEG400+50%H2O
For best results, use promptly after mixing.
0.375mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 464.82
Formula

C21H16ClF3N4O3

CAS No. 284461-73-0
Storage powder
in solvent
Synonyms BAY 43-9006
Smiles CNC(=O)C1=NC=CC(=C1)OC2=CC=C(C=C2)NC(=O)NC3=CC(=C(C=C3)Cl)C(F)(F)F

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04000737 Recruiting Drug: YIV-906+Sorafenib|Drug: Placebo+Sorafenib Advanced Hepatocellular Carcinoma Yiviva Inc. January 10 2020 Phase 2
NCT03958669 Recruiting -- Hepatocellular Carcinoma|Sorafenib University Hospital Tuebingen|German Federal Ministry of Education and Research November 1 2019 --
NCT03764293 Recruiting Drug: SHR-1210|Drug: Apatinib|Drug: Sorafenib Locally Advanced or Metastatic and Unresectable HCC Jiangsu HengRui Medicine Co. Ltd. June 10 2019 Phase 3
NCT03582618 Recruiting Drug: Sorafenib|Drug: CVM-1118 Hepatocellular Carcinoma|Advanced Cancer TaiRx Inc. July 12 2018 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Raf Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID