Sorafenib (BAY 43-9006)

Catalog No.S7397 Synonyms: NSC-724772

For research use only.

Sorafenib (BAY 43-9006, NSC-724772) is a multikinase inhibitor of Raf-1 and B-Raf with IC50 of 6 nM and 22 nM in cell-free assays, respectively. Sorafenib inhibits VEGFR-2, VEGFR-3, PDGFR-β, Flt-3 and c-KIT with IC50 of 90 nM, 20 nM, 57 nM, 59 nM and 68 nM, respectively. Sorafenib induces autophagy and apoptosis and activates ferroptosis with anti-tumor activity.

Sorafenib (BAY 43-9006) Chemical Structure

CAS No. 284461-73-0

Selleck's Sorafenib (BAY 43-9006) has been cited by 437 publications

Purity & Quality Control

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Biological Activity

Description Sorafenib (BAY 43-9006, NSC-724772) is a multikinase inhibitor of Raf-1 and B-Raf with IC50 of 6 nM and 22 nM in cell-free assays, respectively. Sorafenib inhibits VEGFR-2, VEGFR-3, PDGFR-β, Flt-3 and c-KIT with IC50 of 90 nM, 20 nM, 57 nM, 59 nM and 68 nM, respectively. Sorafenib induces autophagy and apoptosis and activates ferroptosis with anti-tumor activity.
Targets
Raf-1 [1]
(Cell-free assay)
mVEGFR2(Flk1) [1]
(Cell-free assay)
mVEGFR3 [1]
(Cell-free assay)
B-Raf [1]
(Cell-free assay)
B-Raf (V599E) [1]
(Cell-free assay)
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6 nM 15 nM 20 nM 22 nM 38 nM
In vitro

Sorafenib inhibits both wild-type and V599E mutant B-Raf activity with IC50 of 22 nM and 38 nM, respectively. Sorafenib also potently inhibits mVEGFR2 (Flk-1), mVEGFR3, mPDGFRβ, Flt3, and c-Kit with IC50 of 15 nM, 20 nM, 57 nM, 58 nM, and 68 nM, respectively. Sorafenib weakly inhibits FGFR-1 with IC50 of 580 nM. Sorafenib tosylate is not active against ERK-1, MEK-1, EGFR, HER-2, IGFR-1, c-Met, PKB, PKA, cdk1/cyclinB, PKCα, PKCγ, and pim-1. Sorafenib markedly inhibits VEGFR2 phosphorylation in NIH 3T3 cells with IC50 of 30 nM, and Flt-3 phosphorylation in HEK-293 cells with IC50 of 20 nM. Sorafenib potently blocks MEK 1/2 and ERK 1/2 phosphorylation in most cell lines but not in A549 or H460 cells, while having no effect on inhibition of the PKB pathway. Sorafenib inhibits the proliferation of HAoSMC and MDA-MB-231 cells with IC50 of 0.28 μM and 2.6 μM, respectively. [1] In addition to inhibition of the RAF/MEK/ERK signaling pathway, Sorafenib significantly inhibits the phosphorylation of eIF4E and down-regulates Mcl-1 levels in hepatocellular carcinoma (HCC) cells in a MEK/ERK-independent manner. Sorafenib inhibits the proliferation of PLC/PRF/5 and HepG2 cells with IC50 of 6.3 μM and 4.5 μM, respectively, and leads to the significant induction of apoptosis. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MV-4-11 Mlv4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7FeJJMUUN3ME2wMlAxODByM{CzJO69VQ>? NWDlVZdVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN|Nj:nQnNCVkeHUkyvZV4>
MONO-MAC-6 M3\hOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjtTWM2OD1yLkCwOFE5KM7:TR?= M4HWXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{OzZxJ{7TRW5ITVJ:L3G+
ALL-PO MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTBwMEOxPFQh|ryP NGTjdIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzN4Lze+V2FPT0WUPD;hQi=>
NKM-1 MmL3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkD3TWM2OD1yLkC3OFE3KM7:TR?= M1fx[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{OzZxJ{7TRW5ITVJ:L3G+
CGTH-W-1 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTBwMkWwNlIh|ryP Mo\6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVM{Pi9pPmPBUmdGWjxxYU6=
BB65-RCC MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVrsS3E6UUN3ME2wMlQ4ODd|IN88US=> NY\xOI55RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN|Nj:nQnNCVkeHUkyvZV4>
NOS-1 M17sZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1P3c2lEPTB;MD61OlM3KM7:TR?= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxN|M3Nyd-U1HOS2VTRC:jPh?=
SH-4 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYC2UmVuUUN3ME2wMlY2PjF|IN88US=> MliyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVM{Pi9pPmPBUmdGWjxxYU6=
HOP-62 M1vmWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXlNm95UUN3ME2wMlg2ODh6IN88US=> NHnBS4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzN4Lze+V2FPT0WUPD;hQi=>
HCC2998 M4jT[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYS2SpA{UUN3ME2wMlg5QDF6IN88US=> MnTtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVM{Pi9pPmPBUmdGWjxxYU6=
GDM-1 NH\lfXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPvRmVKSzVyPUCuPVA3QThizszN NXG5ZnJnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN|Nj:nQnNCVkeHUkyvZV4>
KM12 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3;R[WlEPTB;MT6wNlA6QCEQvF2= M2PndVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{OzZxJ{7TRW5ITVJ:L3G+
LB2518-MEL Mn\ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXpNIY6UUN3ME2xMlIxQDB7IN88US=> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxN|M3Nyd-U1HOS2VTRC:jPh?=
NCI-H1436 NGrpXXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG[zV|ZKSzVyPUGuNlE3PzhizszN NWPpPHhXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN|Nj:nQnNCVkeHUkyvZV4>
EM-2 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTFwM{W1O|gh|ryP MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxN|M3Nyd-U1HOS2VTRC:jPh?=
LAMA-84 M1HESmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYDD[XdbUUN3ME2xMlM4PjR6IN88US=> MoPIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVM{Pi9pPmPBUmdGWjxxYU6=
KG-1 NEjuR45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3\1PWlEPTB;MT60O|k{PSEQvF2= M1nxdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{OzZxJ{7TRW5ITVJ:L3G+
A388 NX7tSXNvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2PRNWlEPTB;MT61PVE3PSEQvF2= NV\POmZ7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN|Nj:nQnNCVkeHUkyvZV4>
no-10 M1S2eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIjySHhKSzVyPUGuOlE4OjZizszN M1HVdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{OzZxJ{7TRW5ITVJ:L3G+
SF126 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXfJR|UxRTFwNkO4NVIh|ryP NGHWRpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzN4Lze+V2FPT0WUPD;hQi=>
MEG-01 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWqyeZJtUUN3ME2xMlgxQThizszN NHfjdoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzN4Lze+V2FPT0WUPD;hQi=>
A3-KAW M4Pw[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTFwOEi0NkDPxE1? NIHEfnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzN4Lze+V2FPT0WUPD;hQi=>
D-247MG M3jUUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnZTWM2OD1{LkG0OFgh|ryP NXmxWoxyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN|Nj:nQnNCVkeHUkyvZV4>
OVCAR-4 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkfCTWM2OD1{LkKxN|k{KM7:TR?= NIrtSYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzN4Lze+V2FPT0WUPD;hQi=>
NCI-SNU-1 NIDUToRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoHsTWM2OD1{LkOxOlIh|ryP NXKxelg3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN|Nj:nQnNCVkeHUkyvZV4>
NCI-H2171 NUTiNllXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFrEdmdKSzVyPUKuN|k4PjRizszN M{TDV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{OzZxJ{7TRW5ITVJ:L3G+
SIG-M5 NWT5TWJmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDoTWM2OD1{LkSyNlQzKM7:TR?= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxN|M3Nyd-U1HOS2VTRC:jPh?=
BE-13 NHr6UIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MofzTWM2OD1{Lk[5OlA6KM7:TR?= NVP0ZYN1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN|Nj:nQnNCVkeHUkyvZV4>
K052 NXHPWI5HT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnPZTWM2OD1{Lke0OlE3KM7:TR?= M2nh[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{OzZxJ{7TRW5ITVJ:L3G+
L-540 NIDHTFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfwdZl2UUN3ME2yMlc2Pzh7IN88US=> M13tbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{OzZxJ{7TRW5ITVJ:L3G+
KMOE-2 NXj4fWNMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1TnSWlEPTB;Mj64NVM2KM7:TR?= NFHIblM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzN4Lze+V2FPT0WUPD;hQi=>
MFH-ino Ml[0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW\JR|UxRTJwOUKxPFUh|ryP M3PadVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{OzZxJ{7TRW5ITVJ:L3G+
HL-60 M37PUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnRTWM2OD1|LkC2Nlk6KM7:TR?= M160Z|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{OzZxJ{7TRW5ITVJ:L3G+
HCC2218 NInvWpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEn5T3VKSzVyPUOuNVIxODNizszN M1TFPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{OzZxJ{7TRW5ITVJ:L3G+
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MZ1-PC Mle3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;GTWM2OD1|LkS3OVA6KM7:TR?= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxN|M3Nyd-U1HOS2VTRC:jPh?=
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BC-1 MnPuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTNwN{SwNkDPxE1? NX2xUmFpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN|Nj:nQnNCVkeHUkyvZV4>
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MV4-11 MYPDfZRwfG:6aXPpeJkh[XO|YYm= NFe3W21KSzVyIE2gNE4xODdizszN Mn75QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|NEK4OlcoRjJ4M{SyPFY4RC:jPh?=
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MV4-11 NVO0c45[S3m2b4TvfIlkcXS7IHHzd4F6 MXHHTVUxKD1iMD6wOFMh|ryP NXnnRVV5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NVg4ODlpPkKzOlE5PzB7PD;hQi=>
MV4-11 NHXyV4dHfW6ldHnvckBie3OjeR?= MVLHTVUxKD1iMD6wOFMh|ryP MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MUCyN{c,OjZyOEGwNlM9N2F-
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MOLM-13 MV3GeY5kfGmxbjDhd5NigQ>? NFntWmFIUTVyIE2gNE4xPTZizszN MkjlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOEGwNlMoRjJ4MEixNFI{RC:jPh?=
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Spodoptera frugiperda Ml62SpVv[3Srb36gZZN{[Xl? NWm4VmlQUUN3MDC9JFAvODlizszN NUPpOnJ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5NlIzOjhpPkK2PVIzOjJ6PD;hQi=>
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MV4-11 Mo\6RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgbJVu[W5? MXPJR|UxKD1iMD6zJO69VQ>? NWTlTlhpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyOFI2PTNpPkK4NlQzPTV|PD;hQi=>
HepG2 NGDXRmVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MYXFR|UxKD1iMD6zNFIh|ryP MmLhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUC4NVAoRjJ5MEGwPFExRC:jPh?=
THP1 NInqblBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= Mo\xTWM2OCB;IECuN|Eh|ryP NVW2bnRURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1O|A2OjZpPkKwOVcxPTJ4PD;hQi=>
SMMC7721 MoH5R5l1d3SxeHnjbZR6KGG|c3H5 M3;seGlEPTBiPTCwMlM4KM7:TR?= NX7z[Gc1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5PFIxPzVpPkK1PVgzODd3PD;hQi=>
HT-29 MWjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? Ml\VTWM2OCB;IECuN|kh|ryP NHLuVJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe3PFk6PSd-MkW3O|g6QTV:L3G+
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A549 NInrO2NEgXSxdH;4bYNqfHliYYPzZZk> MV7JR|UxKD1iMD62N{DPxE1? NXrmZmJuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5PFIxPzVpPkK1PVgzODd3PD;hQi=>
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WM3629 MWjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NH;vT3hIUTVyIE2gNE43PSEQvF2= MnLmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2Nk[1OFIoRjJyNE[2OVQzRC:jPh?=
SMMC7721 M3TLZmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 Ml32TWM2OCB;IECuOlUh|ryP NHPJT449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO0NlE{PCd-Mk[zOFIyOzR:L3G+
WM3629 M4XvT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2X1Z2dKPTBiPTCwMlc5KM7:TR?= NXXh[4J{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFk3PThpPkKwNVQ6PjV6PD;hQi=>
WM3629 MWHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NUX0XlJoT0l3MDC9JFAvPzhizszN NH;Benk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkixNFI3OCd-Mk[4NVAzPjB:L3G+
MCF7 MkLyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MV\JR|UxKD1iMD63PEDPxE1? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd5OEm5OUc,OjV5N{i5PVU9N2F-
K562 MWrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NHLBTW9KSzVyIE2gNE45PiEQvF2= MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDNzNUG5Nkc,OjR|MUWxPVI9N2F-
COLO205 NI\hW2RCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NEm1PVJKSzVyIE2gNE45PyEQvF2= M4fFT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{e4PVk2Lz5{NUe3PFk6PTxxYU6=
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MDA-MB-231 M4LEOmN6fG:2b4jpZ4l1gSCjc4PhfS=> M1\ob2lEPTBiPTCwMlk1KM7:TR?= M33rPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEi2NlM5Lz5{NUC4OlI{QDxxYU6=
MDA-MB-231 MVjDfZRwfG:6aXPpeJkh[XO|YYm= MV7JR|UxKD1iMD65OEDPxE1? NGqwOmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS0NFg4QSd-MkW0OFA5Pzl:L3G+
HepG2 NXvnTVZMSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MWrJR|UxKD1iMT6wOkDPxE1? M2\1cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkK4N|I2Lz5{OU[yPFMzPTxxYU6=
BL21(DE3) MnTmSpVv[3Srb36gZZN{[Xl? Mmf0TWM2OCB;IEGuNUDPxE1? MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl{OEi1PEc,OTl7Mki4OVg9N2F-
K562 NFvTRmZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MkXSTWM2OCB;IEGuNlIh|ryP M3LUbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{e4PVk2Lz5{NUe3PFk6PTxxYU6=
MDA-MB-231 MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkfpS2k2OCB;IEGuNlYh|ryP NGTHS5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
MDA-MB-231 NVj4ZmRLS3m2b4TvfIlkcXS7IHHzd4F6 NHLZXIpIUTVyIE2gNU4zPiEQvF2= M2X4UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmwOVA5Lz5{NkW5NFUxQDxxYU6=
MDA-MB-231 M2foeGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M1TG[WdKPTBiPTCxMlI3KM7:TR?= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
SK-MEL-28 NF7XXG1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MmC4SWM2OCB;IEGuN{DPxE1? NYXuTmlRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi5OFI5OjdpPkG4PVQzQDJ5PD;hQi=>
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A549 NFnRdFBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkLvTWM2OCB;IEOuNUDPxE1? MmD3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzMEKxO|UoRjJ7MUCyNVc2RC:jPh?=
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A549/ATCC MkC4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4Tv[mdKPTBiPTCzMlE3KM7:TR?= NGS5PVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
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DU145 M124Wmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M17rRmdKPTBiPTCzMlE3KM7:TR?= MomwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
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BT549 M1ftRWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mki3S2k2OCB;IEOuNVYh|ryP MkjlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
K562 Mlu3R5l1d3SxeHnjbZR6KGG|c3H5 MoHkS2k2OCB;IEOuNVYh|ryP MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
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A549 MkDTR5l1d3SxeHnjbZR6KGG|c3H5 MnXtS2k2OCB;IEOuNVYh|ryP NXrUS2dxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
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SNB75 M1nZfWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NF7ib4xIUTVyIE2gN{4yPiEQvF2= M3HheVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
OVCAR3 MXXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MULHTVUxKD1iMz6xOkDPxE1? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
SNB19 NHnKRmtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MlvIS2k2OCB;IEOuNVYh|ryP MmrOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
SK-MEL-5 NXzVTJpVSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MVfUS2khRSB|LkG2JO69VQ>? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
OVCAR4 NHrBPVNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MX\HTVUxKD1iMz6xOkDPxE1? M2LxUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
SK-MEL-2 MX3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NXH3PYh2XEeLIE2gN{4yPiEQvF2= NYjrXIx3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
BT549 NHToV4NCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MYfHTVUxKD1iMz6xOkDPxE1? MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
MOLT4 NHe0NHhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M4DCV2dKPTBiPTCzMlE3KM7:TR?= Ml6wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
K562 MUjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NVLscIhVT0l3MDC9JFMvOTZizszN MlzGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
HCC2998 NV36bIx[SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NITi[pRIUTVyIE2gN{4yPiEQvF2= NXLyWG5lRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
HCC2998 M4XkOGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M2[1cnRIUSB;IEOuNVYh|ryP MoK3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
A549/ATCC M1P3[mFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MljlS2k2OCB;IEOuNVYh|ryP MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
DU145 M37PSGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MnL1S2k2OCB;IEOuNVYh|ryP MkTZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
SR NH\0R2FCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MV;HTVUxKD1iMz6xOkDPxE1? M2rjS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
MCF7 NWDuXWdSS3m2b4TvfIlkcXS7IHHzd4F6 NFvoNFJKSzVyIE2gN{4yQCEQvF2= NF;ZSHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEmyO|gxOSd-Mki5Nlc5ODF:L3G+
PC3 Mo\ER5l1d3SxeHnjbZR6KGG|c3H5 NVzsOGtXUUN3MDC9JFMvOThizszN Mon5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7Mke4NFEoRjJ6OUK3PFAyRC:jPh?=
MCF7 NV;pb|A1S3m2b4TvfIlkcXS7IHHzd4F6 MUnJR|UxKD1iMz6xPEDPxE1? MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd5N{CwPUc,Ojd5N{ewNFk9N2F-
A549 MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NEXHe5BKSzVyIE2gN{4yQSEQvF2= M3rpSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{e4PVk2Lz5{NUe3PFk6PTxxYU6=
PC3 NUjBTWpKS3m2b4TvfIlkcXS7IHHzd4F6 M2TvcGlEPTBiPTCzMlI1KM7:TR?= NYDOSIJTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke3O|cxODlpPkK3O|c4ODB7PD;hQi=>
HeLa MorCSpVv[3Srb36gZZN{[Xl? M1fLNmlEPTBiPTCzMlMh|ryP NH\DTpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUKyOVcxPid-MUWyNlU4ODZ:L3G+
HeLa MYnGeY5kfGmxbjDhd5NigQ>? M1jqR2lEPTBiPTCzMlMh|ryP NW\ORXVVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWyNlU4ODZpPkG1NlI2PzB4PD;hQi=>
LoVo M3nUcGN6fG:2b4jpZ4l1gSCjc4PhfS=> MorvTWM2OCB;IEOuN{DPxE1? M2PWZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEW0NFE4Lz5{M{S1OFAyPzxxYU6=
A375 NV;acJhqSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NWLhSHdzUUN3MDC9JFMvOzZizszN Mm\xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4MEK2O|QoRjJ7NkCyOlc1RC:jPh?=
HT-29 M3jiOWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NVnrXW5wUUN3MDC9JFMvOzdizszN M3\xNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUmxPVM5Lz5{Nkm5NVk{QDxxYU6=
FLT3 gene-deficient U937 NVnoZZd[SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M3m1TGdKPTBiPTCzMlQh|ryP MnLaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MEi0OlgoRjJzN{C4OFY5RC:jPh?=
FLT3 negative U937 NEjicIJEgXSxdH;4bYNqfHliYYPzZZk> M1HmZmdKPTBiPTCzMlQh|ryP M3L5eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkG4O|A6Lz5{M{[xPFcxQTxxYU6=
U937 MmHCR5l1d3SxeHnjbZR6KGG|c3H5 NUGxWYZKT0l3MDC9JFMvPCEQvF2= NIKx[WU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NVAzOyd-Mk[wPFExOjN:L3G+
A375P MYrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? Mo[wS2k2OCB;IEOuOEDPxE1? NUTTUJZGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0OVkyPDRpPkK5OFU6OTR2PD;hQi=>
ACHN M163O2N6fG:2b4jpZ4l1gSCjc4PhfS=> M2jDNGlEPTBiPTCzMlQh|ryP NWTkNJZpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1NVc6ODhpPkK5OVE4QTB6PD;hQi=>
ACHN MlixR5l1d3SxeHnjbZR6KGG|c3H5 MojwTWM2OCB;IEOuOEDPxE1? NYjPS49wRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyPVc3QDhpPkK5Nlk4Pjh6PD;hQi=>
HepG2 M4PyZ2Z2dmO2aX;uJIF{e2G7 M3rFd2lEPTBiPTCzMlQh|ryP NWXH[mNsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wO|E5PjFpPkK2NFcyQDZzPD;hQi=>
A375P MUTDfZRwfG:6aXPpeJkh[XO|YYm= MkLRS2k2OCB;IEOuOEDPxE1? M2L2OlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEe4NVk{Lz5{NEi3PFE6OzxxYU6=
HepG2 MoXvR5l1d3SxeHnjbZR6KGG|c3H5 NHTKOWZKSzVyIE2gN{41PCEQvF2= NH\nNFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEmyO|gxOSd-Mki5Nlc5ODF:L3G+
HepG2 NFnxSnBEgXSxdH;4bYNqfHliYYPzZZk> MVHJR|UxKD1iMz60OEDPxE1? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd5N{CwPUc,Ojd5N{ewNFk9N2F-
ACHN M13RZmN6fG:2b4jpZ4l1gSCjc4PhfS=> M2D0Z2lEPTBiPTCzMlUh|ryP Mk\4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5OE[0N|YoRjJ7N{i2OFM3RC:jPh?=
HepG2 NVn1UFNmSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M{LrdmlEPTBiPTCzMlUh|ryP MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MkK2OUc,OjV2NkKyOlU9N2F-
HT-29 NUS4bHhjS3m2b4TvfIlkcXS7IHHzd4F6 NGXuV5lKSzVyIE2gN{43OSEQvF2= NWHBNFdrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NlY5OTVpPkK0PFI3QDF3PD;hQi=>
HT-29 M2rvbmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 Ml;JTWM2OCB;IEOuOlEh|ryP MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJzNki0PUc,OzB{MU[4OFk9N2F-
HT-29 NGftd5lEgXSxdH;4bYNqfHliYYPzZZk> M1;hXWlEPTBiPTCzMlYyKM7:TR?= MkK3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyOE[yN|goRjJ3MEi2NlM5RC:jPh?=
HT-29 NFHOe2JEgXSxdH;4bYNqfHliYYPzZZk> MV;JR|UxKD1iMz62NUDPxE1? M{i5UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NESwPFc6Lz5{NUS0NFg4QTxxYU6=
MCF7 NUK1WXZDSW62aXPhcoNmeiCjY4Tpeol1gSCjc4PhfS=> NVXtTWw4UUN3MDC9JFMvPjRizszN NEjkW2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0PVg1OSd-Mkm1OFk5PDF:L3G+
Sf9 NEO0S5RHfW6ldHnvckBie3OjeR?= NVnQdWxCUUN3MDC9JFMvQCEQvF2= NUPlW4R7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NFg1PjhpPkKxO|A5PDZ6PD;hQi=>
Sf9 NHj6d|hHfW6ldHnvckBie3OjeR?= M1nvOGlEPTBiPTCzMlgh|ryP NX3CW4ZERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NVg4ODlpPkKzOlE5PzB7PD;hQi=>
Sf9 NITOUmlHfW6ldHnvckBie3OjeR?= M1;jdGlEPTBiPTCzMlgh|ryP NY[4blRkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFExOjNpPkK2NFgyODJ|PD;hQi=>
A2058 NISwVFFEgXSxdH;4bYNqfHliYYPzZZk> NV;jXpI2UUN3MDC9JFMvQCEQvF2= NYLiPVNXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3NFg6QDdpPkKyO|A5QTh5PD;hQi=>
NCI-H460 NUC0fVdjS3m2b4TvfIlkcXS7IHHzd4F6 NEP4[FlKSzVyIE2gN{46KM7:TR?= NF;nS2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1OFAyPyd-MkO0OVQxOTd:L3G+
SK-MEL-30 M2TCbmN6fG:2b4jpZ4l1gSCjc4PhfS=> MXzJR|UxKD1iMz65JO69VQ>? MoT5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2NkG4NlcoRjJ7NE[xPFI4RC:jPh?=
HT-29 MVTBcpRq[2GwY3XyJIFkfGm4aYT5JIF{e2G7 NYLaPXRXUUN3MDC9JFMvQTdizszN NGrIVpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0PVg1OSd-Mkm1OFk5PDF:L3G+
SF268 NWXRNZhNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlTaWGdKKD1iMz65PEDPxE1? NH23T2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
SNB75 NIDG[llIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVvDVZFbXEeLIE2gN{46QCEQvF2= NIPtZ|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
OVCAR3 MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnjpWGdKKD1iMz65PEDPxE1? NXP2e5VPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
SNB19 MnzDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mo\MWGdKKD1iMz65PEDPxE1? NVfOWZdrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
Hs 578T M{XJTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkP6WGdKKD1iMz65PEDPxE1? NHHhWJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
TK10 NYTkNYl3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWW3dW5wT0l3MDC9JFMvQThizszN MmPoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
BT549 NHjFNZZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEGye|BVT0liPTCzMlk5KM7:TR?= MlHqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
Caki1 M1W5eGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MUXUS2khRSB|Lkm4JO69VQ>? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
TK10 M1;MOWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MkTuS2k2OCB;IEOuPVgh|ryP MmLhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
MDA-MB-435 MlW1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MXjUS2khRSB|Lkm4JO69VQ>? NYPsZ4xQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
HCT116 NEPEdopCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MoLYWGdKKD1iMz65PEDPxE1? NVLST4cxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
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HepG2 NFXVPFlEgXSxdH;4bYNqfHliYYPzZZk> NIfYR2NKSzVyIE2gOEDPxE1? NVrrNYc5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVQxOTdpPkKzOFU1ODF5PD;hQi=>
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MDA-MB-436 M4rjUmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NH\McIlKSzVyIE2gOE4zKM7:TR?= M4XHSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MkCyOFA{Lz5{OUKwNlQxOzxxYU6=
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OVCAR4 Ml\JR5l1d3SxeHnjbZR6KGG|c3H5 NEe4TmxVT0liPTC0MlMyKM7:TR?= NGnYeI89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
WM1361 M{fXfmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M3z2cmdKPTBiPTC0MlM1PSEQvF2= NGDINno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0PFU3Oyd-MkCxOFg2PjN:L3G+
WM266.4 NU\VUHpUT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXXHTVUxKD1iND61JO69VQ>? Mn;vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzOUmwPFcoRjJyMUm5NFg4RC:jPh?=
HepG2 M{LzN2N6fG:2b4jpZ4l1gSCjc4PhfS=> MkDFS2k2OCB;IESuOUDPxE1? NILQfXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{eyOlAzQCd-MkO3NlYxOjh:L3G+
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LS174T NVv1O3M1TnWwY4Tpc44h[XO|YYm= M2\5OmlEPTBiPTC0MlUzKM7:TR?= NYi5dVJ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyOlY6OzdpPkK5NlY3QTN5PD;hQi=>
8505C NXfPeHhRSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NVX4cFh6UUN3MDC9JFQvPyEQvF2= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB|MkCzNUc,OjlyM{KwN|E9N2F-
OVCAR8 NXHIXGhyS3m2b4TvfIlkcXS7IHHzd4F6 Mk\jWGdKKD1iND63N{DPxE1? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
MDA-MB-231 MVrDfZRwfG:6aXPpeJkh[XO|YYm= MWnJR|UxKD1iND63O{DPxE1? M3\aU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEO1OFc6Lz5{MESzOVQ4QTxxYU6=
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WM164 M3zaRWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MVPJR|UxKD1iND65JO69VQ>? NH7Eb4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W2NVM6Oid-MUe1OlE{QTJ:L3G+
B16-F1 NH7YPW1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MYLJR|UxKD1iND65JO69VQ>? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB3NkW0PEc,OjByNU[1OFg9N2F-
MDA-MB-468 NH7UemVEgXSxdH;4bYNqfHliYYPzZZk> MXPJR|UxKD1iND65JO69VQ>? NIHNcHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG1PVQ5Oyd-Mk[xOVk1QDN:L3G+
786-O Ml32R5l1d3SxeHnjbZR6KGG|c3H5 Ml\6TWM2OCB;IESuPUDPxE1? Ml;BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3MUe5NFgoRjJ7NUG3PVA5RC:jPh?=
786-O Ml;SR5l1d3SxeHnjbZR6KGG|c3H5 Mkn2TWM2OCB;IESuPUDPxE1? M4C3S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7Mkm3Olg5Lz5{OUK5O|Y5QDxxYU6=
WM266.4 M3[xNWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MUPHTVUxKD1iND65N|Mh|ryP MnHoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEi1OlMoRjJyMUS4OVY{RC:jPh?=
WM266.4 MmrPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NETtXXFKSzVyIE2gOUDPxE1? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN{M{W2NEc,OTl|MkO1OlA9N2F-
WM164 MnPrRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NFzHdY9KSzVyIE2gOUDPxE1? MlfsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjByNU[1OFgoRjJyMEW2OVQ5RC:jPh?=
HepG2 MoG3RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M4PWRWlEPTBiPTC1JO69VQ>? MljVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NkC1O|goRjJ|Mk[wOVc5RC:jPh?=
HepG2 NWPCPWhuSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NVTTZmFIUUN3MDC9JFUh|ryP NGPlbFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mzNlA4OSd-MkO5N|IxPzF:L3G+
NCI-H23 NVvDdFVWT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWSyNIMyXEeLIE2gOU4xOSEQvF2= Ml[0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
NCI-H322M M4frSWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2LrVnRIUSB;IEWuNFEh|ryP MnLNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
NCI-H460 M2K5PGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlfHWGdKKD1iNT6wNUDPxE1? MnHaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
NCI-H522 MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NF3MXVRVT0liPTC1MlAyKM7:TR?= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
HT-29 MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmCxWGdKKD1iNT6wNUDPxE1? NUnvS2RTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
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HOP92 NIXaXpJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVHUS2khRSB3LkCxJO69VQ>? M3fCclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
786-0 NWLV[nBjT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NESxfGVVT0liPTC1MlAyKM7:TR?= M{Du[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
MDA-MB-435 MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVLjOmF{XEeLIE2gOU4xOSEQvF2= NFS0[mo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
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UACC257 MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYjPTHVqXEeLIE2gOU4xOSEQvF2= NVHmVmpFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
SKOV3 M3HHZWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVjUS2khRSB3LkCxJO69VQ>? M{HU[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
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UACC62 NW\ac5V5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NIXRXolVT0liPTC1MlAyKM7:TR?= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
UACC257 NUTnZm04SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NH\MellVT0liPTC1MlAyKM7:TR?= M2PTU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
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M14 MW\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NIHtcoxVT0liPTC1MlAyKM7:TR?= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
NCI-H23 NXOzS4NzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NWfmfIFwXEeLIE2gOU4xOSEQvF2= M1fISFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
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OS-RC2 M3PuRWN6fG:2b4jpZ4l1gSCjc4PhfS=> NXn3cpBHUUN3MDC9JFch|ryP NX24V4FJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1NVc6ODhpPkK5OVE4QTB6PD;hQi=>
OS-RC2 NVW4VZFVS3m2b4TvfIlkcXS7IHHzd4F6 MVXJR|UxKD1iNzFOwG0> Mo\rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{OUe2PFgoRjJ7Mkm3Olg5RC:jPh?=
COLO205 NF;Udo1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBpfW2jbh?= MWrJR|UxKD1iNz6wOEDPxE1? M1HXVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkSyOVU{Lz5{OEK0NlU2OzxxYU6=
rhabdomyosarcoma NHS0U|FEgXSxdH;4bYNqfHliYXP0bZZqfHliYXfhbY5{fCCqdX3hci=> NGniS|FESzVyIE2gO{4xPSEQvF2= NU\1c5RbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkeyPFgyQDZpPkK3Nlg5OTh4PD;hQi=>
HuH7 NH;xSnVEgXSxdH;4bYNqfHliYYPzZZk> M3HQe2lEPTBiPTC3MlEh|ryP MmfNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzNUm0PFMoRjJ4MUW5OFg{RC:jPh?=
MDA-MB-231 NEXmb|FCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NGjQUplKSzVyIE2gO{4yQCEQvF2= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd{MUmyOEc,OjJ5MkG5NlQ9N2F-
FLT3-negative K562 MXrDfZRwfG:6aXPpeJkh[XO|YYm= MlvaS2k2OCB;IEeuN{DPxE1? NYrrNm1wRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NVg4ODlpPkKzOlE5PzB7PD;hQi=>
K562 Mk\2SpVv[3Srb36gZZN{[Xl? MnjIS2k2OCB;IEeuN{DPxE1? Ml3EQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOEGwNlMoRjJ4MEixNFI{RC:jPh?=
K562 NV;kbGViTnWwY4Tpc44h[XO|YYm= M4jVZ2dKPTBiPTC3MlMh|ryP MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MUCyN{c,OjZyOEGwNlM9N2F-
MCF7 MmTvRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MWnJR|UxKD1iNz6zN{DPxE1? NXf4b3FXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2OFQzOTlpPkKzOlQ1OjF7PD;hQi=>
A549 MnzhR5l1d3SxeHnjbZR6KGG|c3H5 NGXFOnNVT0liPTC3MlM3KM7:TR?= NUHSW|lPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
WI38 Mn\oR5l1d3SxeHnjbZR6KGG|c3H5 MYXJR|UxKD1iNz61OEDPxE1? MmXDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6Mk[4NVUoRjJ2OEK2PFE2RC:jPh?=
A375 MVjDfZRwfG:6aXPpeJkh[XO|YYm= MVzJR|UxKD1iNz61OkDPxE1? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJzNUixPEc,OjR{MUW4NVg9N2F-
MDA-MB-231 M3\M[WN6fG:2b4jpZ4l1gSCjc4PhfS=> NG\5UFBKSzVyIE2gO{43OiEQvF2= MoC5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzOEG0NVQoRjJyMUixOFE1RC:jPh?=
786-0 MUDDfZRwfG:6aXPpeJkh[XO|YYm= MkTRWGdKKD1iNz62NkDPxE1? MmX0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
HS27 M1TaPWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NH3STFlKSzVyIE2gO{45KM7:TR?= MmLqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6NUe5OlMoRjF7OEW3PVY{RC:jPh?=
HS27 MmLZR5l1d3SxeHnjbZR6KGG|c3H5 M3fiO2lEPTBiPTC3Mlgh|ryP MlnBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6OUezOlYoRjF7OEm3N|Y3RC:jPh?=
HCT116 MUDDfZRwfG:6aXPpeJkh[XO|YYm= M1HEOWlEPTBiPTC3Mlgh|ryP M3[2RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEizOVkzLz5{MkS4N|U6OjxxYU6=
HS27 M1G5TGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NUjLeWI2UUN3MDC9JFcvQDVizszN NEL6c289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS2OFg5Pyd-MUm0OlQ5QDd:L3G+
HS27 MWXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? Mm\hS2k2OCB;IEeuPFUh|ryP M4j5XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{WzOVcyLz5{MUO1N|U4OTxxYU6=
A549/ATCC NG\GNJlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3rmd3RIUSB;IEeuPVQh|ryP NXfzdJk4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
DU145 NUKyU3pbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1O4dnRIUSB;IEeuPVQh|ryP NHX6S|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
NCI/ADR-RES M{DrW2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVvHRlRGXEeLIE2gO{46PCEQvF2= NWPLUG9IRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
MCF7 M1fhdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYLpNHBxXEeLIE2gO{46PCEQvF2= NHf2PVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
PC3 M3HtSmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M4r5bHRIUSB;IEeuPVQh|ryP MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
UO31 MofRRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MXPUS2khRSB5Lkm0JO69VQ>? MofVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
NCI-ADR-RES NEnUPYRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NIHBOZpVT0liPTC3Mlk1KM7:TR?= NF7zPJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
LOXIMVI M2L0c2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M1jLPGxEPTBiPTC3Mlk1KM7:TR?= NULlXZNyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
SK-MEL-28 NFvxbIJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M3vu[HRIUSB;IEeuPVQh|ryP M{H4VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
KM12 MonSRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M3j0WXRIUSB;IEeuPVQh|ryP MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
COLO205 NX;qSVRESW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NIPXO5dVT0liPTC3Mlk1KM7:TR?= NGDTT289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
HT-29 M4TmdmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M{CxS3RIUSB;IEeuPVQh|ryP MmjoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
NCI-H226 NHyw[nlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MnnJWGdKKD1iNz65OEDPxE1? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
HOP62 MorpRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MlrvWGdKKD1iNz65OEDPxE1? M1PrUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
T47D Mni0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NYLYR4NpXEeLIE2gO{46PCEQvF2= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
MDA-MB-468 M4Li[mFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MUXUS2khRSB5Lkm0JO69VQ>? MkXjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
EKVX MlLxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NX\pNVF7XEeLIE2gO{46PCEQvF2= MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
MCF7 NYLrb|IxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MlzmWGdKKD1iNz65OEDPxE1? NELZXmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
Hs 578T MVLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MlXPWGdKKD1iNz65OEDPxE1? NWfpdnBSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
MGC803 NFn3SohCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MkOwTWM2OCB;IEeuPVkh|ryP MoHOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3NkCwOFkoRjJ4NU[wNFQ6RC:jPh?=
HCT116 NHG0ZXlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MlXKTWM2OCB;IEiuNFgh|ryP MlXqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NEC0O|koRjJ2NESwOFc6RC:jPh?=
PC3 NVrWd4YxS3m2b4TvfIlkcXS7IHHzd4F6 MUHJR|UxKD1iOD6wPEDPxE1? M4nYbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MESzNlY5Lz5{N{C0N|I3QDxxYU6=
HCT116 M3f3dmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NWmyWIJGUUN3MDC9JFgvODhizszN M3jOSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkS0NlE6Lz5{M{[0OFIyQTxxYU6=
WM266.4 Mm\oRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M4\oVmdKPTBiPTC4MlEh|ryP MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB{NUm5Okc,OjNyMkW5PVY9N2F-
WM266.4 NVHyVmViSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MXLHTVUxKD1iOD6xNkDPxE1? M4TvSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUizOlY6Lz5{MkW4N|Y3QTxxYU6=
Ketr3 NFq1Tm1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NH7Uco1KSzVyIE2gPE4zPyEQvF2= Mn;2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{MU[4OFkoRjNyMkG2PFQ6RC:jPh?=
A375 NUe0NXRrSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MYDJR|UxKD1iOD6yO{DPxE1? NFLkOXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEKxOlg1QSd-M{CyNVY5PDl:L3G+
HepG2 MY\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M1;RZmlEPTBiPTC4MlI4KM7:TR?= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJzNki0PUc,OzB{MU[4OFk9N2F-
MX1 NVXOTVJySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M2jRWGlEPTBiPTC4MlI4KM7:TR?= NYLJeGNlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyNVY5PDlpPkOwNlE3QDR7PD;hQi=>
MX1 Mof5R5l1d3SxeHnjbZR6KGG|c3H5 M4[3WGlEPTBiPTC4MlI4KM7:TR?= MkHnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4N{WxN|UoRjJ2Nke1NVM2RC:jPh?=
WM266.4 NHPYeWtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUexbHQ3T0l3MDC9JFgvOyEQvF2= NF\2coQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkO2NVY5Pid-MkKzOlE3QDZ:L3G+
PLC/PRF/5 NHLoTXJEgXSxdH;4bYNqfHliYYPzZZk> M1\hb2lEPTBiPTC4MlMh|ryP Mn3xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3M{GwOVMoRjJzNUOxNFU{RC:jPh?=
A375 NY\jfXpMSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NYKxVGJsUUN3MDC9JFgvOzNizszN Mk\xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl6OE[zNlQoRjJ7OEi2N|I1RC:jPh?=
HCT116 Mn7sR5l1d3SxeHnjbZR6KGG|c3H5 MX\JR|UxKD1iOD60NUDPxE1? MnqxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4M{G3PFgoRjJ7NkOxO|g5RC:jPh?=
WI38 NFfjW2VEgXSxdH;4bYNqfHliYYPzZZk> M1HUe2lEPTBiPTC4MlQzKM7:TR?= NYfE[JdMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5PVE6OzhpPkK2PVkyQTN6PD;hQi=>
HepG2 M2XPemN6fG:2b4jpZ4l1gSCjc4PhfS=> M370U2lEPTBiPTC4MlQzKM7:TR?= M{\0WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MU[yNVI{Lz5{N{G2NlEzOzxxYU6=
HepG2 NUnmeYc1S3m2b4TvfIlkcXS7IHHzd4F6 MlfDTWM2OCB;IEiuOFIh|ryP MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5MUmwPUc,OjN6N{G5NFk9N2F-
HT-29 M{PPSmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NUDiRpExUUN3MDC9JFgvPDRizszN M2jT[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkCyOlc1Lz5{OU[wNlY4PDxxYU6=
HepG2 MVLDfZRwfG:6aXPpeJkh[XO|YYm= NFPwR3JKSzVyIE2gPE43PyEQvF2= M2jBUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Nke1NVM2Lz5{NE[3OVE{PTxxYU6=
MCF7 NEjidZdEgXSxdH;4bYNqfHliYYPzZZk> NGTIbVlKSzVyIE2gPE45OyEQvF2= M4f5WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEO1OFc6Lz5{MESzOVQ4QTxxYU6=
MCF7 M2fBPGN6fG:2b4jpZ4l1gSCjc4PhfS=> MkPmTWM2OCB;IEmuNVIh|ryP NX3zUItjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNFA6OjBpPkK0N|AxQTJyPD;hQi=>
HepG2 NHXGem5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NH3IWmdKSzVyIE2gPU4yPCEQvF2= NIG0ZWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW2NFA1QSd-Mk[1OlAxPDl:L3G+
A375 M1;YTGN6fG:2b4jpZ4l1gSCjc4PhfS=> NFLEb5VKSzVyIE2gPU4yPyEQvF2= NGe1VYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[3OVE{PSd-MkS2O|UyOzV:L3G+
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SKOV3 M2LVXWN6fG:2b4jpZ4l1gSCjc4PhfS=> M2XLS2lEPTBiPTC5MlI2KM7:TR?= NXTGWpNLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNFA6OjBpPkK0N|AxQTJyPD;hQi=>
RS4:11 MWXGeY5kfGmxbjDhd5NigQ>? M1TCVWdKPTBiPTC5MlMh|ryP Mo\4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOEGwNlMoRjJ4MEixNFI{RC:jPh?=
RS4:11 NUHacm1yS3m2b4TvfIlkcXS7IHHzd4F6 NGnJ[ZJIUTVyIE2gPU4{KM7:TR?= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZzOEewPUc,OjN4MUi3NFk9N2F-
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PANC1 NXPCUmJwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4\zb2lEPTBiPTC5MlMzKM7:TR?= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTFyMkG3OUc,OjlzMEKxO|U9N2F-
MHCC97H NV;0WmE{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NF\wRoNKSzVyIE2gPU4{OiEQvF2= M{fwO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUCyNVc2Lz5{OUGwNlE4PTxxYU6=
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Hep3B NYiye21WS3m2b4TvfIlkcXS7IHHzd4F6 MoP0TWM2OCB;IEmuOEDPxE1? NVzPdXdLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki4OlUzPzZpPkK4PFY2Ojd4PD;hQi=>
LoVo NHu3XYZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXLJR|UxKD1iOT60O{DPxE1? M4DQVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUCyNVc2Lz5{OUGwNlE4PTxxYU6=
SMMC7721 MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M3zvc2lEPTBiPTC5Mlk3KM7:TR?= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ|N{GyN{c,OjV4M{exNlM9N2F-
MCF7 M1fBOmN6fG:2b4jpZ4l1gSCjc4PhfS=> NEH5OJNVT0liPTC5Mlk4KM7:TR?= NG\UNXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
IGROV1 MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2fj[HRIUSB;IEGwJO69VQ>? M4WyWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
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TK10 MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3;JWHRIUSB;IEGwJO69VQ>? M{DPR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
786-0 M2XnUmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NWLybIIyXEeLIE2gNVAh|ryP NU\sdpdQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
SN12C NYXyd4xvSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NGS4ZpVVT0liPTCxNEDPxE1? M37xVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
Caki1 NUnlWmV7SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NVjYeHczVEN3MDC9JFExKM7:TR?= M2i0TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
TK10 M3\kW2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M3\kRXRIUSB;IEGwJO69VQ>? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
RXF393 M4G4NmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MnLVWGdKKD1iMUCg{txO MlfwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
OVCAR8 M4POTmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M2rNbnRIUSB;IEGwJO69VQ>? M1nTO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
SNB75 MojmRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NFL0SWJVT0liPTCxNEDPxE1? NU\menE{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
NCI-H322M NIXOfWtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NX7nb5FoXEeLIE2gNVAh|ryP M3HhSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
MDA-MB-435 M{P1UGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 Mlf2UGM2OCB;IEGwJO69VQ>? M2[0VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
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SF268 MVrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MWLUS2khRSBzMDFOwG0> Mn;EQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
NCI-H460 NGHrbXFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NYPuW3R2XEeLIE2gNVAh|ryP MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
DU145 NGnjXGtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M2e3OHRIUSB;IEGwJO69VQ>? MoriQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
K1 NGD6bXJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M3rpVWlEPTBiPTCxNE4zKM7:TR?= NX6xZZpxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmwN|IxOzFpPkK5NFMzODNzPD;hQi=>
Bel7402 NGW0UoJEgXSxdH;4bYNqfHliYYPzZZk> M2HrT2lEPTBiPTCxNE4zPiEQvF2= NILRXFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWwOFIxPCd-MkG1NFQzODR:L3G+
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SGC7901 M4jVd2N6fG:2b4jpZ4l1gSCjc4PhfS=> MUDJR|UxKD1iMUCuN|ch|ryP NVzyV4NGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0N|U1PzlpPkKwOFM2PDd7PD;hQi=>
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A431 NUfHbZhPSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M3vjd2lEPTBiPTCxNE41PiEQvF2= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB|MkCzNUc,OjlyM{KwN|E9N2F-
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GES-1 MUDDfZRwfG:6aXPpeJkh[XO|YYm= M1P6O2lEPTBiPTCxNE43QCEQvF2= NYOxW|RRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexOlIyOjNpPkK3NVYzOTJ|PD;hQi=>
H460 NFraVYlEgXSxdH;4bYNqfHliYYPzZZk> NVrKd2VKUUN3MDC9JFExNjhizszN NUjGO4JORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNFA6OjBpPkK0N|AxQTJyPD;hQi=>
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Hep3B M3nveGN6fG:2b4jpZ4l1gSCjc4PhfS=> M2qye2lEPTBiPTCxNU4zKM7:TR?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDN2MkSwNEc,Ojh|NEK0NFA9N2F-
MCF7 Mnu2R5l1d3SxeHnjbZR6KGG|c3H5 M3rrZmlEPTBiPTCxNU4{PCEQvF2= NWP0XnI6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexOlIyOjNpPkK3NVYzOTJ|PD;hQi=>
MCF7 NYX3c5JES3m2b4TvfIlkcXS7IHHzd4F6 MoL1TWM2OCB;IEGxMlM1KM7:TR?= M1[4dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEexPVA6Lz5{M{i3NVkxQTxxYU6=
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HepG2 NWHuW2FVS3m2b4TvfIlkcXS7IHHzd4F6 Mkj4TWM2OCB;IEGxMlQ6KM7:TR?= MonWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MESyNFQoRjJzNUC0NlA1RC:jPh?=
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SGC7901 MoCyR5l1d3SxeHnjbZR6KGG|c3H5 MnX3TWM2OCB;IEGxMlUh|ryP NYLIbGpMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNFA6OjBpPkK0N|AxQTJyPD;hQi=>
HeLa NIq0bnZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NFe0TFVKSzVyIE2gNVIvODFizszN MmrLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzMEO4O|MoRjJ7MUCzPFc{RC:jPh?=
PANC1 NVr5SmtUS3m2b4TvfIlkcXS7IHHzd4F6 NYqyfYx{UUN3MDC9JFEzNjNizszN MlLDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MEC5NlAoRjJ2M{CwPVIxRC:jPh?=
HepG2 NF3PdXZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M3fROmlEPTBiPTCxNk42PCEQvF2= M2GwUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MEOyNFMyLz5{OUCzNlA{OTxxYU6=
A549 NXPLfVVuSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MWLJR|UxKD1iMUKuOVQh|ryP NHWzNos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[0OFIyQSd-MkO2OFQzOTl:L3G+
Caki1 NYjjZnRFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIXQO5FVT0liPTCxNk42QSEQvF2= MlTMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
OVCAR8 M2\sZ2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4\vU3RIUSB;IEGyMlU6KM7:TR?= NHzmVWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
SKOV3 NUTwOFhGSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MlTXWGdKKD1iMUKuOkDPxE1? MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
ACHN NWTyelZYSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M3j6VXRIUSB;IEGyMlYh|ryP MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
OVCAR3 NFKwWWtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M3XM[HRIUSB;IEGyMlYh|ryP NFjWXY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
OVCAR4 MnjMRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MlGyWGdKKD1iMUKuOkDPxE1? NV3XUm1{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
HCT15 Mo\TRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M330WnRIUSB;IEGyMlYh|ryP NILuZnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
SW620 NUDtO5VKSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M3izT3RIUSB;IEGyMlYh|ryP M3XXSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
A549/ATCC MkjURY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NYHncFRjXEeLIE2gNVIvPiEQvF2= NFHDNVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
MDA-MB-468 MVjDfZRwfG:6aXPpeJkh[XO|YYm= NF[wfGZVT0liPTCxN{4xQSEQvF2= NF;3NnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
SK-MEL-28 NFzKblFEgXSxdH;4bYNqfHliYYPzZZk> NGS2clRVT0liPTCxN{4{QSEQvF2= MlvvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
A549 M1y0N2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MX7JR|UxKD1iMUOuOlQh|ryP MoPTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3NkCwOFkoRjJ4NU[wNFQ6RC:jPh?=
A375 M1HjSGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIh2dWGw MYHJR|UxKD1iMUOuOlQh|ryP NHfuXZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEK0NlU2Oyd-MkiyOFI2PTN:L3G+
A375 MYDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NHLINYtKSzVyIE2gNVMvPjRizszN NUnGfVJWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OlIzPjdpPkK1OFYzOjZ5PD;hQi=>
K562 MkT5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M{HseGlEPTBiPTCxN{45PSEQvF2= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ2NEKxPUc,OjN4NESyNVk9N2F-
Hep3B MYHDfZRwfG:6aXPpeJkh[XO|YYm= NXPiNWluUUN3MDC9JFE1NjB6IN88US=> MoHDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MESyNFQoRjJzNUC0NlA1RC:jPh?=
T47D NUPaWldxS3m2b4TvfIlkcXS7IHHzd4F6 M{\aN3RIUSB;IEG0MlM5KM7:TR?= MnXUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
NCI-H226 NVLNZoJQS3m2b4TvfIlkcXS7IHHzd4F6 MorLWGdKKD1iMUSuOFIh|ryP MlGwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
MDA-MB-231 MorLRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MWDJR|UxKD1iMUSuOlIh|ryP MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR2MES3PUc,OjR2NEC0O|k9N2F-
MDA-MB-231 NYLHUm9CSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MUnJR|UxKD1iMUSuOlIh|ryP M3jxdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkS0NlE6Lz5{M{[0OFIyQTxxYU6=
NCI-H522 NHLxSIZEgXSxdH;4bYNqfHliYYPzZZk> NHvvd5ZVT0liPTCxOE45OiEQvF2= Mlj1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
SK-MEL-2 NWnhcoYyS3m2b4TvfIlkcXS7IHHzd4F6 MnHJWGdKKD1iMUSuPFUh|ryP NVzRTpBpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
OS-RC2 NEfvSVZEgXSxdH;4bYNqfHliYYPzZZk> MoO5TWM2OCB;IEG1JO69VQ>? MorwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5OE[0N|YoRjJ7N{i2OFM3RC:jPh?=
HepG2 NHHLTIFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MljpTWM2OCB;IEG1JO69VQ>? NVz1bYN6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxNFM5PzNpPkK5NVA{QDd|PD;hQi=>
fibroblast NXKzbItISW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M1\VNmlEPTBiPTCxOU4yKM7:TR?= M{PFXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEe3OVA2Lz5zOES3O|UxPTxxYU6=
HT-29 MofjR5l1d3SxeHnjbZR6KGG|c3H5 MX;JR|UxKD1iMUWuNkDPxE1? M{nqRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Nke1NVM2Lz5{NE[3OVE{PTxxYU6=
U87MG MUfDfZRwfG:6aXPpeJkh[XO|YYm= NFHBVohKSzVyIE2gNVUvPTdizszN MofpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6Mk[4NVUoRjJ2OEK2PFE2RC:jPh?=
BT549 NWH0SGc6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MUnMR|UxKD1iMUWuPEDPxE1? M3O5UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
GES-1 NWrNSHhCS3m2b4TvfIlkcXS7IHHzd4F6 NXrPNJJjUUN3MDC9JFE2NjhizszN NUDGU5RVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4O|E6ODlpPkKzPFcyQTB7PD;hQi=>
SNB19 NF7ON3lCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NFHnV2FVT0liPTCxOU45PSEQvF2= NE[1OZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
IGROV1 NYH6PHdFSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MWXUS2khRSBzNT65JO69VQ>? NVXsXnFHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
HCT116 NWjzXGFHSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MoHtUGM2OCB;IEG1Mlkh|ryP NWjKbWhmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
OS-RC2 NGLkS5REgXSxdH;4bYNqfHliYYPzZZk> NFjrSJFKSzVyIE2gNVYh|ryP NH7OSnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S1OFAyPyd-MkO0OVQxOTd:L3G+
A375 M{LOemN6fG:2b4jpZ4l1gSCjc4PhfS=> NUnMeYRbUUN3MDC9JFE3NjJ2IN88US=> MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MUOxPEc,OjV2NkGzNVg9N2F-
SKOV3 NH3BU2lEgXSxdH;4bYNqfHliYYPzZZk> Ml7oWGdKKD1iMU[uN|Yh|ryP MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
WM1361 M4rxfmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M16zN2lEPTBiPTCxOk41PCEQvF2= NXjZeHhwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2NFI3PzRpPkK5OlAzPjd2PD;hQi=>
MKN28 M{H5ZmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MYPJR|UxKD1iMUeg{txO MkXiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyM{KwN|EoRjJ7MEOyNFMyRC:jPh?=
SMMC7721 MkjCR5l1d3SxeHnjbZR6KGG|c3H5 M3\ORmlEPTBiPTCxO{4{KM7:TR?= NYm0[phCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NFQzODRpPkKxOVA1OjB2PD;hQi=>
UACC257 MnOyR5l1d3SxeHnjbZR6KGG|c3H5 NITi[4NVT0liPTCxO{4{OyEQvF2= NGrSPVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
EAhy926 NXO3fZF4SW62aXHu[4lw\2WwaXOgZYN1cX[rdImgZZN{[Xl? NYXPdpI5UUN3MDC9JFE5NjV{IN88US=> NUfn[WE2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmwN|IxOzFpPkK5NFMzODNzPD;hQi=>
SMMC7721 MXXDfZRwfG:6aXPpeJkh[XO|YYm= MXnJR|UxKD1iMUiuO{DPxE1? MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDNyMEmyNEc,OjR|MEC5NlA9N2F-
Ketr3 M2DYbWN6fG:2b4jpZ4l1gSCjc4PhfS=> M1:yeGlEPTBiPTCxPE45KM7:TR?= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ5NUGzOUc,OjR4N{WxN|U9N2F-
NCI-H522 NV3ZeZhJS3m2b4TvfIlkcXS7IHHzd4F6 MlnITWM2OCB;IEG5MlI3KM7:TR?= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MUOxPEc,OjV2NkGzNVg9N2F-
A549 NVvmdWNQS3m2b4TvfIlkcXS7IHHzd4F6 M3S3R2lEPTBiPTCxPU42PCEQvF2= NVHsToNWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexOlIyOjNpPkK3NVYzOTJ|PD;hQi=>
SH-SY5Y NVm5c2h4S3m2b4TvfIlkcXS7IHHzd4F6 M3X6fGlEPTBiPTCxPU42PCEQvF2= NYLHZoVyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4O|E6ODlpPkKzPFcyQTB7PD;hQi=>
MGC803 MnjHRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MofSTWM2OCB;IEG5MlkzKM7:TR?= MmP0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzMEO4O|MoRjJ7MUCzPFc{RC:jPh?=
EKVX Ml3iS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXH2WFhEXEeLIE2gNVkvQTVizszN NFTjfYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
OVCAR5 NYXGdmdRSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NWC2R3MzXEeLIE2gNlAh|ryP NE\UPYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
UACC62 NFrCTnZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M3nlTWxEPTBiPTCyNEDPxE1? NGfEWHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
UACC257 NEn2WWdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NUPkXHRJVEN3MDC9JFIxKM7:TR?= NWnmUZJTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
MDA-MB-231 MWrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NXzROYxpVEN3MDC9JFIxKM7:TR?= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
U87 NXX0SG1GS3m2b4TvfIlkcXS7IHHzd4F6 MYDJR|UxKD1iMkGuNFch|ryP M13QVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEO1OFc6Lz5{MESzOVQ4QTxxYU6=
EJ NHXsclVEgXSxdH;4bYNqfHliYYPzZZk> M{fh[WlEPTBiPTCyNk46KM7:TR?= M2DWPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{CwPVIxLz5{NEOwNFkzODxxYU6=
HCT116 NVXW[olPSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M{CwfmlEPTBiPTCyN{4{OSEQvF2= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZyMk[3OEc,Ojl4MEK2O|Q9N2F-
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Assay
Methods Test Index PMID
Western blot LC3-I / LC-3II / ATG5 ; p-STAT3 / STAT3 / Mcl-1 ; β-catenin / Survivin / Mcl-1 / PTMA ; pERK / ERK ; p-PKM2(y105) / PMK2 / Caspase-9 ; RET(pY1016) / VEGFR2(pY1214) / MEK1(pT292) / ERK(pY204) ; Cyclin D1 23392173 26517516 22286758 26959741 22941289 26039995
Growth inhibition assay Cell viability 26039995
Immunofluorescence p65 ; cytochrome c 22286758 22278289
ELISA TGF-beta / CD206 ; Caspase-9 / Caspase-3 26158762 30923462
In vivo Oral administration of Sorafenib (~60 mg/kg) demonstrates broad spectrum, dose-dependent anti-tumor activity against a variety of human tumor xenograft models including MDA-MB-231, Colo-205, HT-29, DLD-1, NCI-H460, and A549, with no evidence of toxicity. In association with the anti-tumor efficacy, Sorafenib treatment potently inhibits MEK 1/2 phosphorylation and pERK 1/2 levels in HT-29 and MDA-MB-231 xenografts but not in Colo-205 xenografts, and significantly suppresses tumor microvessel area (MVA) and microvessel density (MVD) in MDA MB-231, HT-29 and Colo-205 tumor xenografts. [1] Sorafenib treatment produces dose-dependent growth inhibition of PLC/PRF/5 tumor xenografts in SCID mice with TGIs of 49% and 78% at 10 mg/kg and 30 mg/kg, respectively, consistent with the inhibition of ERK and eIF4E phosphorylation, reduction of the microvessel area, and induction of tumor cell apoptosis. [2] Sorafenib sensitizes bax-/- cells to TRAIL in a dose-dependent manner, through a mechanism involving down-regulating NF-κB mediated Mcl-1 and cIAP2 expression. Combining Sorafenib (30-60 mg/kg) with TRAIL (5 mg/kg) show dramatic efficacy in TRAIL-resistant HCT116 bax-/- and HT29 tumor xenografts. [3]

Protocol (from reference)

Kinase Assay:

[1]

  • Biochemical assays:

    Recombinant baculoviruses expressing Raf-1 (residues 305–648) and B-Raf (residues 409–765) are purified as fusion proteins. Full-length human MEK-1 is generated by PCR and purified as a fusion protein from Escherichia coli lysates. Sorafenib tosylate is added to a mixture of Raf-1 (80 ng), or B-Raf (80 ng) with MEK-1 (1 μg) in assay buffer [20 mM Tris (pH 8.2), 100 mM NaCl, 5 mM MgCl2, and 0.15% β-mercaptoethanol] at a final concentration of 1% DMSO. The Raf kinase assay (final volume of 50 μL) is initiated by adding 25 μL of 10 μM γ[33P]ATP (400 Ci/mol) and incubated at 32 °C for 25 minutes. Phosphorylated MEK-1 is harvested by filtration onto a phosphocellulose mat, and 1% phosphoric acid is used to wash away unbound radioactivity. After drying by microwave heating, a β-plate counter is used to quantify filter-bound radioactivity. Human VEGFR2 (KDR) kinase domain is expressed and purified from Sf9 lysates. Time-resolved fluorescence energy transfer assays for VEGFR2 are performed in 96-well opaque plates in the time-resolved fluorescence energy transfer format. Final reaction conditions are as follows: 1 to 10 μM ATP, 25 nM poly GT-biotin, 2 nM Europium-labeled phospho (p)-Tyr antibody (PY20), 10 nM APC, 1 to 7 nM cytoplasmic kinase domain in final concentrations of 1% DMSO, 50 mM HEPES (pH 7.5), 10 mM MgCl2, 0.1 mM EDTA, 0.015% Brij-35, 0.1 mg/mL BSA, and 0.1% β-mercaptoethanol. Reaction volumes are 100 μL and are initiated by addition of enzyme. Plates are read at both 615 and 665 nM on a Perkin-Elmer VictorV Multilabel counter at ~1.5 to 2.0 hours after reaction initiation. Signal is calculated as a ratio: (665 nm/615 nM) × 10,000 for each well. For IC50 generation, Sorafenib tosylate is added before the enzyme initiation. A 50-fold stock plate is made with Sorafenib tosylate serially diluted 1:3 in a 50% DMSO/50% distilled water solution. Final Sorafenib tosylate concentrations range from 10 μM to 4.56 nM in 1% DMSO.

Cell Research:

[1]

  • Cell lines: MDA-MB-231, and HAoSMC
  • Concentrations: Dissolved in DMSO, final concentrations ~10 μM
  • Incubation Time: 72 hours
  • Method:

    Cells are exposed to increasing concentrations of Sorafenib tosylate for 72 hours. Cell number is quantitated using the Cell TiterGlo ATP Luminescent assay kit. This assay measures the number of viable cells per well by measurement of luminescent signal based on amount of cellular ATP.

Animal Research:

[1]

  • Animal Models: Female NCr-nu/nu mice implanted s.c. with MDA-MB-231, Colo-205, HT-29, H460, or A549 cells
  • Dosages: ~60 mg/kg
  • Administration: Orally once daily

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5%DMSO+45%PEG400+50%H2O
For best results, use promptly after mixing.

0.375mg/mL

Chemical Information

Molecular Weight 464.82
Formula

C21H16ClF3N4O3

CAS No. 284461-73-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CNC(=O)C1=NC=CC(=C1)OC2=CC=C(C=C2)NC(=O)NC3=CC(=C(C=C3)Cl)C(F)(F)F

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05068752 Recruiting Drug: Vemurafenib|Drug: Sorafenib Pancreas Cancer HonorHealth Research Institute|Bayer|Genentech Inc. October 28 2021 Phase 2
NCT04763408 Recruiting Drug: Lenvatinib|Drug: Sorafenib Carcinoma Hepatocellular Eisai Inc. April 9 2021 --
NCT04000737 Recruiting Drug: YIV-906+Sorafenib|Drug: Placebo+Sorafenib Advanced Hepatocellular Carcinoma Yiviva Inc. January 10 2020 Phase 2
NCT03958669 Unknown status -- Hepatocellular Carcinoma|Sorafenib University Hospital Tuebingen|German Federal Ministry of Education and Research November 1 2019 --
NCT03764293 Active not recruiting Drug: SHR-1210|Drug: Apatinib|Drug: Sorafenib Locally Advanced or Metastatic and Unresectable HCC Jiangsu HengRui Medicine Co. Ltd. June 10 2019 Phase 3
NCT03582618 Active not recruiting Drug: Sorafenib|Drug: CVM-1118 Hepatocellular Carcinoma|Advanced Cancer TaiRx Inc. July 12 2018 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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