JSH-23

Catalog No.S7351 Batch:S735101

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Technical Data

Formula

C16H20N2
Molecular Weight 240.34 CAS No. 749886-87-1
Solubility (25°C)* In vitro DMSO 48 mg/mL (199.71 mM)
Ethanol 20 mg/mL (83.21 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO Corn oil
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 of 7.1 μM in RAW 264.7 cell line.
Targets
NF-κB [1]
(RAW 264.7 cells)
7.1 μM
In vitro JSH-23 inhibits LPS-induced nuclear translocation of NF-κB p65 without affecting IκBα degradation. JSH-23 inhibits LPS-induced apoptotic chromatin condensation, while does not show significant cytotoxic effects on the RAW 264.7 cells at <100 μM. [1] JSH-23 also decreases NO production and neuronal migration in LPS activated cultures primary cultures from developing mouse cerebellum. [2] Moreover, JSH-23 augments cisplatin cytotoxicity in ovarian cancer cells with CI values ranging from 0.35 to 0.85. [3]
In vivo JSH-23 (3 mg/kg) significantly reverses the nerve conduction and nerve blood flow deficits by decreasing neuroinflammation and improving antioxidant defence in diabetic rats. [4]

Protocol (from reference)

Kinase Assay:[1]
  • Measurement of NF-κB transcriptional activity

    Macrophages RAW 264.7 transfected stably with reporter plasmid of pNF-κB-SEAP-NPT are treated with 1 μg/ml LPS and/or sample for 16 hours. As the reporter, SEAP activity in the cell-free culture media is measured as followed. Single cell-derived stable transfectants are plated in 5 ml of T-25 flask, and the media is decanted 24 h later. At this time, cells are washed twice with phosphate-buffered saline, and incubations are initiated by addition of new media. Chemicals are added to the culture medium after 24 h of incubations. Aliquots (25 ml) of medium from a control or chemical-treated cultures are taken at 0, 3, 20, 24, 48, and 72 h, heated at 65°C for 5 min to eliminate the alkaline phosphatase activity, and used immediately or stored at -20°C. Mixtures consisting of dilution buffer (25 ml), assay buffer (97 ml), culture media (25 ml), and 4-methylumbelliferyl phosphate (MUP, 1 mM, 3 ml) in each well of the 96-well plates are incubated for 60 min in the dark at room temperature. Fluorescence emits the product of the SEAP/MUP is measured at 449 nm using a 96-well plate fluorometer after excitation at 360 nm.

Cell Assay:[1]
  • Cell lines

    RAW 264.7 cells

  • Concentrations

    ~300 μM

  • Incubation Time

    24 hours

  • Method

    Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 compound for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.
Animal Study:[4]
  • Animal Models

    STZ-induced diabetic rats

  • Dosages

    ~3 mg/kg

  • Administration

    Oral administration

Customer Product Validation

Data from [Data independently produced by , , Cell, 2018, 172(4):841-856]

Data from [Data independently produced by , , Blood, 2015, 125(7): 1098-106 ]

Data from [Data independently produced by , , J Mol Med (Berl), 2018, 96(3-4):301-313]

Data from [Data independently produced by , , Biochim Biophys Acta, 2015, 1852(12):2671-7]

Selleck's JSH-23 has been cited by 148 publications

Nlrc3 signaling is indispensable for hematopoietic stem cell emergence via Notch signaling in vertebrates [ Nat Commun, 2024, 15(1):226] PubMed: 38172511
Free heme induces neuroinflammation and cognitive impairment by microglial activation via the TLR4/MyD88/NF-κB signaling pathway [ Cell Commun Signal, 2024, 22(1):16] PubMed: 38183122
TP53 gain-of-function mutations promote osimertinib resistance via TNF-α-NF-κB signaling in EGFR-mutated lung cancer [ NPJ Precis Oncol, 2024, 8(1):60] PubMed: 38431700
ERK1/2-CEBPB Axis-Regulated hBD1 Enhances Anti-Tuberculosis Capacity in Alveolar Type II Epithelial Cells [ Int J Mol Sci, 2024, 25(4)2408] PubMed: 38397085
FGF18 alleviates sepsis-induced acute lung injury by inhibiting the NF-κB pathway [ Respir Res, 2024, 25(1):108] PubMed: 38419044
Extensive jejunal injury is repaired by migration and transdifferentiation of ileal enterocytes in zebrafish [ Cell Rep, 2023, 42(7):112660] PubMed: 37342912
Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO [ Cell Mol Biol Lett, 2023, 28(1):62] PubMed: 37525118
Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO [ Cell Mol Biol Lett, 2023, 28(1):62] PubMed: 37525118
BMP8B Activates Both SMAD2/3 and NF-κB Signals to Inhibit the Differentiation of 3T3-L1 Preadipocytes into Mature Adipocytes [ Nutrients, 2023, 16(1)64] PubMed: 38201894
IL-33/ST2 axis of human amnion fibroblasts participates in inflammatory reactions at parturition [ Mol Med, 2023, 29(1):88] PubMed: 37403020

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.