Home Metabolism P450 (e.g. CYP17) chemical Danshensu

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Danshensu P450 (e.g. CYP17) chemical

Cat.No.S4741

Danshensu (Salvianic acid A), a herbal preparation used in traditional Chinese medicine, possesses potential antitumor and anti‑angiogenesis effects. This compound inhibits CYP2E1 and CYP2C9 with IC50 of 36.63 and 75.76 μm, respectively.
Danshensu P450 (e.g. CYP17) chemical Chemical Structure

Chemical Structure

Molecular Weight: 198.17

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Quality Control

Batch: Purity: 99.68%
99.68

Solubility

In vitro
Batch:

Water : 10 mg/mL

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : Insoluble

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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Chemical Information, Storage & Stability

Molecular Weight 198.17 Formula

C9H10O5

 Storage (From the date of receipt)
CAS No. 76822-21-4 Download SDF Storage of Stock Solutions

Synonyms Salvianic acid A Smiles C1=CC(=C(C=C1CC(C(=O)O)O)O)O

Mechanism of Action

Targets/IC50/Ki
CYP2E1
(Cell-free assay)
36.63 μM
CYP2C9
(Cell-free assay)
75.76 μM
In vitro

Danshensu reduces lipid peroxidation on mitochondrial membrane by scavenging free radicals, and inhibits permeability and transmission of mitochondrial membrane by reducing thiol oxidation. This compound markedly improves cell viability and decreased lactate dehydrogenase (LDH) release in H9c2 cardiomyocytes. It increases phosphorylation of Akt and extracellular signal-related kinase 1/2 (ERK1/2) in H9c2 cells, and the protective effects are partially inhibited by phosphatidylinositol 3'-kinase (PI3K) specific inhibitor wortmannin or ERK specific inhibitor U0126. This chemical could provide significant cardioprotection against MI/R injury, and the potential mechanisms might to suppression of cardiomyocytes apoptosis through activating the PI3K/Akt and ERK1/2 signaling pathways. It increases Bcl-2 expression and decreases Bax, active caspase-3 expression by activating Akt and ERK signaling pathways. This compound has been demonstrated to have biological activities in improving microcirculation, suppressing the formation of reactive oxygen species, inhibiting platelet adhesion and aggregation, protecting myocardium against ischemia, protecting endothelial cells against injury induced by inflammation.
In vivo

Pretreatment with danshensu in ISO-administered rats shows a significant (P<0.001) decrease in ST-segment as compared to ISO-administered rats. Its pretreatment also shows significant (P<0.001) decrease in the levels of serum cTnI when compared to the ISO. Thus, this compound exerts significant cardioprotective effects against ISO-induced myocardial infarction in rats. In the rat model of MI/R injury, this chemical significantly reduces myocardium infarct size and the production of creatine kinase-MB (CK-MB), cardiac troponin (cTnI) in serum.
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