Carprofen

Catalog No.S4136

For research use only.

Carprofen inhibits canine COX2 with IC50 of 30 nM.

Carprofen Chemical Structure

CAS No. 53716-49-7

Purity & Quality Control

Choose Selective COX Inhibitors

Other COX Products

Biological Activity

Description Carprofen inhibits canine COX2 with IC50 of 30 nM.
Features Potency of carprofen for canine COX2 is more than 100-fold greater relative to canine COX1.
Targets
canine COX2 [1]
30 nM
In vitro

Carprofen (S and R stereoisomers) inhibits canine COX2 with IC50 of 0.102 microM for the racemic mixture, the inhibition is primarily attributable to the S enantiomer (IC50, 0.0371 microM), which is approximately 200-fold more potent than the R enantiomer (IC50, 5.97 microM). [1] Carprofen binds to human serum albumin (HSA) by both fluorescence and equilibrium dialysis (ED) methods is characterized by two sets of association constants [K1 = 5.1 μM (fluorescence) and 3.7 μM (ED), K2 = 37 μM (fluorescence) and 13 μM (ED)]. Carprofen primarily binds to site II, the benzodiazepine site, while the low affinity site of carprofen is site I, the warfarin site, the carboxyl group of carprofen is found to play an important role especially in the high affinity binding of carprofen to HSA. [2]

In vivo Carprofen preoperatively has lower pain scores than the other groups, significantly so at 2 hours postextubation in the dogs. Carprofen (4 mg/kg) results in significant higher maximum plasma concentration, the area under the curve to the last data point, and area under the first moment curve up to the last data point in dogs. [3] Carprofen (4 mg/kg) provides slightly better pain relief than pethidine and produced less sedation in dogs. Carprofen provides good analgesia during the 18 hours the dogs are in hospital and no adverse side effects are observed. [4] Carprofen substantially increases the speed of lame birds, providing evidence that birds with moderate lameness suffer pain when they walk. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 55 mg/mL
(200.94 mM)
Ethanol 55 mg/mL
(200.94 mM)
Water Insoluble

Chemical Information

Molecular Weight 273.71
Formula

C15 H12ClNO2

CAS No. 53716-49-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C1=CC2=C(C=C1)C3=C(N2)C=CC(=C3)Cl)C(=O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Carprofen | Carprofen supplier | purchase Carprofen | Carprofen cost | Carprofen manufacturer | order Carprofen | Carprofen distributor