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Celecoxib (SC 58635)

Name: Celecoxib (SC 58635)
Brand: Selleck Chemicals
SKU: S1261
Rating: 5 (79 reviews)

Licensed by Pfizer Catalog No.S1261

For research use only.

Celecoxib (SC 58635) is a selective COX-2 inhibitor with IC50 of 40 nM in Sf9 cells.

CAS No. 169590-42-5

Selleck's Celecoxib (SC 58635) has been cited by 79 publications

Purity & Quality Control

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COX Signaling Pathway Map

Biological Activity

Description

Celecoxib (SC 58635) is a selective COX-2 inhibitor with IC50 of 40 nM in Sf9 cells.

Targets

COX-2 ^[1]
(Sf9 cells)

40 nM

In vitro

Celecoxib shows low sensitivity against COX-1 with IC50 of 15 μM. ^[1] Celecoxib shows an anti-proliferative effect on nasopharyngeal carcinoma (NPC) cell lines including HNE1 and CNE1-LMP1 with IC50 of 32.86 μM and 61.31 μM, respectively. ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

A549	MorrSpVv[3Srb36gRZN{[Xl?	M3\sTVAvOS1\|MDFOwG0>	M{jKdlQ5KGh?		MmLobY5lfWOnczDJR2FONTFiZYjwdoV{e2mxbjDvckBjd3SqIIDyc5RmcW5iYX7kJI1TVkFibHX2[Yw>	NHnEfXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkWxN\|E4Oid-Mk[1NVMyPzJ:L3G+
H460	MVfGeY5kfGmxbjDBd5NigQ>?	NGTxXmwxNjFvM{Cg{txO	MVu0PEBp		NHL0WYxqdmS3Y3XzJGlESU1vMTDlfJBz\XO\|aX;uJI9vKGKxdHigdJJwfGWrbjDhcoQhdVKQQTDs[ZZmdA>?	NG\tb3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkWxN\|E4Oid-Mk[1NVMyPzJ:L3G+
HKESC-2	NEn1OY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NX3LdJVlOjEEoN88US=>	NWqyTm5wPDkEoHlCpC=>		MlfDd4lodmmoaXPhcpRtgSCrbnPy[YF{\XNidHjlJGlEPTEEoI\hcJVmeyCxZjDvfIFtcXCuYYTpcuKh	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjR5NE[5N{c,OjZ2N{S2PVM9N2F-
CaES-17	NEO3RlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M4jvdVIxyqEQvF2=	MXy0POKhcMLi		M{DrWZNq\26rZnnjZY51dHliaX7jdoVie2W\|IITo[UBKSzVywrD2ZYx2\XNib3[gc5hidGmybHH0bY7DqA>?	MkXIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2N{S2PVMoRjJ4NEe0Olk{RC:jPh?=
HKESC-2	NILx[YtCeG:ydH;zbZMhSXO\|YYm=	MknZNlDDqM7:TR?=	NV\6UVdJPDkEoHlCpC=>		M3vTTJJm\HWlZYOgc5hidGmybHH0bY4ucW6mdXPl[EBieG:ydH;zbZM>	NFLqeYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkS3OFY6Oyd-Mk[0O\|Q3QTN:L3G+
CaES-17	NHTYZlNCeG:ydH;zbZMhSXO\|YYm=	M1m0eFIxyqEQvF2=	M2qwelQ5yqCqwrC=		NWjRXVJSemWmdXPld{BwgGGuaYDsZZRqdi2rbnT1Z4VlKGGyb4D0c5Nqew>?	NGHObWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkS3OFY6Oyd-Mk[0O\|Q3QTN:L3G+
A549	M{PqPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NWrTPFd3PS1zNkCg{txO	NIPFWFA1QMLiaNMg	M4rwXGROW09?	MX3JR\|UxRTF4Mz60JO69VQ>?	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjR4NE[0N{c,OjZ2NkS2OFM9N2F-
HCC827	MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXq1MVE3OCEQvF2=	NH\KOHE1QMLiaNMg	NYLlc2g4TE2VTx?=	NInDemZKSzVyPU[5MlIh\|ryP	NIXEe4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkS2OFY1Oyd-Mk[0OlQ3PDN:L3G+
A549	NV63W2ZZSXCxcITvd4l{KEG\|c3H5	NVjNV4w1QDBiwsXN	NVvGU4RuPDkEoHlCpC=>	NX\BPVhbTE2VTx?=	M4\WR4lv\HWlZYOgZZBweHSxc3nz	MlTyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2NkS2OFMoRjJ4NE[0OlQ{RC:jPh?=
HCC827	MWnBdI9xfG:\|aYOgRZN{[Xl?	NUTsOnBGQDBiwsXN	MW[0POKhcMLi	M2DU[2ROW09?	MkXRbY5lfWOnczDhdI9xfG:\|aYO=	NUj3eYxnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0OlQ3PDNpPkK2OFY1PjR\|PD;hQi=>
SGC-7901/DDP	NXziV2JMTnWwY4Tpc44hSXO\|YYm=	NIjBWZEyOMLiwsXN	MofKNlQhcA>?		M2ruTYlvcGmkaYTzJIN6[2yxb4j5[4Vv[XOnLUKgZY5lKFBvZ3z5Z49xem:2ZXnuJIV5eHKnc4Ppc44>	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjRyN{[1N{c,OjZ2MEe2OVM9N2F-
SGC-7901	MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NVW3fHNmOjUEoHlCpC=>		NHXmSWJKSzVyPUGxOU4xQCEQvF2=	NXTScYx2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NFc3PTNpPkK2OFA4PjV\|PD;hQi=>
SGC-7901/DDP	MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		M33mcFI1yqCqwrC=		MVPJR\|UxRTN3LkS1JO69VQ>?	NYjEOWtbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NFc3PTNpPkK2OFA4PjV\|PD;hQi=>
SGC-7901/DDP	MUjBdI9xfG:\|aYOgRZN{[Xl?	NWroe5p6OTEEoNM1US=>	Mof4NlQhcA>?		NWDjRYNMcW6mdXPld{BieG:ydH;zbZMh[2:vYnnu[YQhf2m2aDD3bZRpKGOrc4DsZZRqdg>?	NXvOOIJYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NFc3PTNpPkK2OFA4PjV\|PD;hQi=>
SGC-7901/DDP	MV7GeY5kfGmxbjDBd5NigQ>?	MXKxNOKhyrWP	MXiyOEBp		M2jpcYlvcGmkaYTzJJRp\SCneIDy[ZN{cW:wIH;mJHAu\2y7Y3;wdo91\WmwIITodo92\2hiYnzvZ4tqdmdiRWCyMEBidmRiaX7hZ5RqfmG2aX7nJHBMSSCjbnSgR3JGSsLi	NWXqbnBoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NFc3PTNpPkK2OFA4PjV\|PD;hQi=>
SGC-7901/DDP	NHrs[2JHfW6ldHnvckBCe3OjeR?=	NEHt[4oyOMLiwsXN	NU\lNG1LOjRiaB?=		MnXUZ4F2e2W\|IHGgcYFzc2WmIHTlZ5Jm[XOnIHnuJJRp\SCuZY\lcEBw\iCEY3ytNkBxem:2ZXnu	M{GzfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NEC3OlU{Lz5{NkSwO\|Y2OzxxYU6=
H357	MkDOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MYi3MlUwOTBizszN	MoTKOFghcA>?	Mn\iSG1UVw>?	NH;X[pJqdmirYnn0d{Bk\WyuIHfyc5d1cMLiY3;tZolv\WRid3n0bEBU[WK3dH;jcIF5	NIHPZpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkCwPVg4PCd-Mk[wNFk5PzR:L3G+
TAF	MmizSpVv[3Srb36gRZN{[Xl?	NGHG[ogyOMLiwsXN	MoLoO{Bl		NEfrfmxi\m[nY4TzJHRCTnNiYXTo[ZNqfmVicILvdIVzfGmncx?=	NX\RcWVlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5PFcyOjdpPkK1PVg4OTJ5PD;hQi=>
TAF	NFLodW1HfW6ldHnvckBCe3OjeR?=	M1fz[\|ExyqEEtV2=	M2j5RlMxNTF{MDDtbY4>		Mo\2dI9wemy7IHHm[oVkfHNiZFHreEBxcG:\|cHjvdplt[XSrb36gZ49ve2WzdXXueEB1dyCHR1[gd4lodmGuaX7n	Ml7uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7OEexNlcoRjJ3OUi3NVI4RC:jPh?=
TAF	NWDWRZB1TnWwY4Tpc44hSXO\|YYm=	NUXtfFdDOTEEoNM1US=>	Mnn0OFghcA>?		NEjudI9qdmO{ZXHz[ZMhTUeIUjDtVm5CKGGwZDDwdo91\WmwIHX4dJJme3Orb36=	NGTqS\|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm4O\|EzPyd-MkW5PFcyOjd:L3G+
PANC-1	NFLSWIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MVOyNE83OC9zMECg{txO	NWHHNVlROjRxNEivO\|IhcA>?		NFXqd3BqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDic5RpKHSrbXWgZY5lKGSxc3Wg[IVx\W6mZX70JI1idm6nch?=	M4TpRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUezNFYzLz5{NUm3N\|A3OjxxYU6=
PANC-1	M3zpZWZ2dmO2aX;uJGF{e2G7	NWm2eGVTOjBxNkCvNVAxKM7:TR?=	NH21UXUzPCCq		NETwdVBifHSnboXheJMh[2WubDDpcpZie2mxbjDhcoQhdWmpcnH0bY9vKGmwIHGgZ49v[2WwdILheIlwdi2mZYDlcoRmdnRibXHucoVzyqB?	NFPySY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm3N\|A3Oid-MkW5O\|MxPjJ:L3G+
HeLa	MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MkjBNlDDqM7:TdMg	MmTaNlTDqGh?		M4m1U4VvcGGwY3XzJJJi\GmjdHnvck1qdmS3Y3XkJIlvcGmkaYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:w	NUK3V4x6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3O\|A1OjNpPkK1O\|cxPDJ\|PD;hQi=>
SACC-83	MnPhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NIfN[YszOMLizszNxsA>	NFHOU3ozPMLiaB?=		Mnj1[Y5p[W6lZYOgdoFlcWG2aX;uMYlv\HWlZXSgbY5pcWKrdHnvckBw\iClZXzsJJBzd2yrZnXyZZRqd25?	NGrTSJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe3NFQzOyd-MkW3O\|A1OjN:L3G+
HeLa	NF;r[5NCeG:ydH;zbZMhSXO\|YYm=	MmLBNlDDqM7:TdMg	NU\3RW82OjUEoHi=		Mors[Y5p[W6lZYOgdoFlcWG2aX;uMYlv\HWlZXSgbY5pcWKrdHnvckBw\iClZXzsJIFxd3C2b4Ppdy=>	NXG4XnEyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3O\|A1OjNpPkK1O\|cxPDJ\|PD;hQi=>
SACC-83	NXTJS\|RISXCxcITvd4l{KEG\|c3H5	M4PjeFIxyqEQvF5CpC=>	MX6yOOKhcA>?		M1P6W4VvcGGwY3XzJJJi\GmjdHnvck1qdmS3Y3XkJIlvcGmkaYTpc44hd2ZiY3XscEBieG:ydH;zbZM>	MljQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5N{C0NlMoRjJ3N{ewOFI{RC:jPh?=
HeLa	MXrGeY5kfGmxbjDBd5NigQ>?	MWmyNE81OC96MDFOwG0>	M{CwSFI1yqCq		NYj1e5NLfXC{ZXf1cIF1eyCSVFXOJJBienSrYXzsfUBjgSCrbnjpZol1cW6pIGPwNUwh[W6mIHHjeIl3[XSnczDQWGVPKGGwZDDpcoFkfGm4YYTld{BCU1R?	MomzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5N{C0NlMoRjJ3N{ewOFI{RC:jPh?=
SACC-83	M3nzcWZ2dmO2aX;uJGF{e2G7	MnPWNlAwPDBxOECg{txO	Mmf6NlTDqGh?		NUHnbJZpfXC{ZXf1cIF1eyCSVFXOJJBienSrYXzsfUBjgSCrbnjpZol1cW6pIGPwNUwh[W6mIHHjeIl3[XSnczDQWGVPKGGwZDDpcoFkfGm4YYTld{BCU1R?	NV7nXY9WRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3O\|A1OjNpPkK1O\|cxPDJ\|PD;hQi=>
HLCZ01	M3zpZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MlmwNQKBmzZywrFCuW0>	NEO3No01QCCq	NX;abFlxTE2VTx?=	NFLPbFFqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meg>?	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd{NEi5PUc,OjV5MkS4PVk9N2F-
HLCZ01	M1jX[mZ2dmO2aX;uJGF{e2G7	MlnhOFDDqM7:TR?=	MoD0NlQhcA>?	NES1eWxFVVOR	MkXRbY5kemWjc3XzJIFk[3WvdXzheIlwdiCxZjDHNE9IOS2yaHHz[UBk\WyuczDjc41jcW6nZDD3bZRpKEmITj5OtS=>	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd{NEi5PUc,OjV5MkS4PVk9N2F-
HLCZ01	MVLBdI9xfG:\|aYOgRZN{[Xl?	Mn7GOFDDqM7:TR?=	M4DBSVI1KGh?	M2HwW2ROW09?	MmDq[Y5p[W6lZYOgZZBweHSxc3nzJINwdWKrbnXkJJdqfGhiSV\OMe6y	NEPBPVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUeyOFg6QSd-MkW3NlQ5QTl:L3G+
HLCZ01	NVPSfo16TnWwY4Tpc44hSXO\|YYm=	MXy0NOKh\|ryP	NXHW[2NoOjRiaB?=	MYLEUXNQ	M3L4UIlv[3KnYYPld{BVWkGLTDDlfJBz\XO\|aX;uJINwdWKrbnXkJJdqfGhiSX70[ZJn\XKxbj5OtS=>	MlHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MkS4PVkoRjJ3N{K0PFk6RC:jPh?=
MGC803	MmDIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MVKwMVYxKM7:TR?=	NXLFUVJzPzJiaB?=		MkHhTWM2OD12Nz6yOgKBkcLz4pEJOk41OyEQvF2=	MlPmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MEGzO\|goRjJ3N{CxN\|c5RC:jPh?=
SGC7901	MmnHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M4Hu[lAuPjBizszN	NXPibVZNPzJiaB?=		NV;xTHh5UUN3ME20N{42O+LCidMx5qCKPS5zMjFOwG0>	NWTuR4NvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NFE{PzhpPkK1O\|AyOzd6PD;hQi=>
MGC803	M2TTemZ2dmO2aX;uJGF{e2G7	NYT3WW1IPDEEoN88US=>	NUP5[WUyQC9zNj:yOEBp		MnrpeZBz\We3bHH0d{BE[mxvYjDlfJBz\XO\|aX;u	NF3hS3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUewNVM4QCd-MkW3NFE{Pzh:L3G+
SGC7901	NXzIRY5pTnWwY4Tpc44hSXO\|YYm=	NUHTXGw2PDEEoN88US=>	MmC0PE8yPi9{NDDo		NXjRPYxQfXC{ZXf1cIF1eyCFYnytZkBmgHC{ZYPzbY9v	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdyMUO3PEc,OjV5MEGzO\|g9N2F-
MCF-7	MoLYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MYGxM\|ExKM7:TR?=	M4PNblczKGh?	NYfkflVnTE2VTx?=	NF3s[G9mdmijbnPld{Bk[WylaYTybY9tNWmwZIXj[YQh[2WubDDndo94fGhiaX7obYJqfGmxbh?=	NU\zcHJXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2Olc2OTBpPkK1OlY4PTFyPD;hQi=>
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MDA-MB-231	M{HiXWZ2dmO2aX;uJGF{e2G7	MXSwMVQxKML3TR?=	MoC1OE0zPCCq		NEjiWVRqdmO{ZXHz[ZMhS0:[LUKgdJJwfGWrbjDlfJBz\XO\|aX;uJIlvKGKxdHigeIlu\SCjbnSg[I9{\SCmZYDlcoRmdnRibXHucoVz	NX;LUmFHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1PFc{OjlpPkK1OVg4OzJ7PD;hQi=>
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SGC-7901	M2G0Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M3mwe\|UxNTF{NTFOwG0>	MnfaNlQwPDhxN{KgbC=>		NGPk[3dqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDic5RpKHSrbXWgZY5lKGSxc3Wg[IVx\W6mZX70JI1idm6nch?=	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ5NkO5OEc,OjR4N{[zPVQ9N2F-
SGC-7901	NXnUR\|dnSXCxcITvd4l{KEG\|c3H5	NHrNOlAyODBizszN	NILa[VE4OiCq		NYPuSGY2cW6mdXPld{BieG:ydH;zbZM>	NIr5fWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[3OlM6PCd-MkS2O\|Y{QTR:L3G+
SGC-7901	Mon1SpVv[3Srb36gRZN{[Xl?	M1HSPFc2NzFyMD:xNlUh\|ryP	NF64S4EzPC92OD:3NkBp		Mo\ObY5kemWjc3XzJINie3Cjc3WtPEBidmRiLUmgcXJPSSCneIDy[ZN{cW:wIHnuJIJwfGhidHnt[UBidmRiZH;z[UBu[W6wZYK=	M{jtfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Nke2N\|k1Lz5{NE[3OlM6PDxxYU6=
LMeC	NFjxN3RHfW6ldHnvckBCe3OjeR?=	MlnRNlAwPTBizszN	MmHhOFghcA>?		NVzvOGtH\GWlcnXhd4V{KHSqZTDlfJBz\XO\|aX;uJI9nKEORWD2yJJBzd3SnaX6=	NWC1T2szRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2OVY4PDZpPkK0OlU3PzR4PD;hQi=>
CMeC-1	NFfpS2hE\WyuIG\pZYJqdGm2eTDBd5NigQ>?	M2fTNlIxNzVyIN88US=>	NHPGPJo1QCCq		NHXkZmdqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wg[IVx\W6mZX70JI1idm6nch?=	MkfhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4NU[3OFYoRjJ2NkW2O\|Q3RC:jPh?=
LMeC	MnX2R4VtdCCYaXHibYxqfHliQYPzZZk>	NEnQfmUzOC93MDFOwG0>	MnW4OFghcA>?		NVy2UWFtcW6qaXLpeJMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlJIRmeGWwZHXueEBu[W6wZYK=	Mn3SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4NU[3OFYoRjJ2NkW2O\|Q3RC:jPh?=
CMeC-1	NHG3dZlHfW6ldHnvckBCe3OjeR?=	NV;udIlMOjBxNUCg{txO	M{DKUVQ5KGh?		MVTpcoR2[2W\|IFexMXMh[XK{ZYP0	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ3Nke0Okc,OjR4NU[3OFY9N2F-
LMeC	NV\yUXpTTnWwY4Tpc44hSXO\|YYm=	M1f6O\|IxNzVyIN88US=>	NFzFNFY1QCCq		MnTvbY5lfWOnczDHNU1UKGG{cnXzeC=>	MlLaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4NU[3OFYoRjJ2NkW2O\|Q3RC:jPh?=
CMeC-1	M3npdGZ2dmO2aX;uJGF{e2G7	MUKyNE82OCEQvF2=	NXP4SJlEPDhiaB?=		MmGz[IVkemWjc3XzJJRp\SCuZY\lcJMhd2ZiY4njcIlvKERzIHnuJIEh\G:\|ZT3k[ZBmdmSnboSgcYFvdmW{	NF[xU4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[1Olc1Pid-MkS2OVY4PDZ:L3G+
LMeC	NX;5OZViTnWwY4Tpc44hSXO\|YYm=	NVrMVXdjOjBxNUCg{txO	NYHuSHREPDhiaB?=		NWH1XlR5\GWlcnXhd4V{KHSqZTDs[ZZmdHNib3[gZ5lkdGmwIFSxJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy	Ml3hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4NU[3OFYoRjJ2NkW2O\|Q3RC:jPh?=
CMeC-1	NXXmeWV[TnWwY4Tpc44hSXO\|YYm=	Mnq1NlAwPTBizszN	MVi0PEBp		NYH3UnlIcW6mdXPld{Bk[XOyYYPlMVMh[WO2aY\heIlwdg>?	MkDKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4NU[3OFYoRjJ2NkW2O\|Q3RC:jPh?=
LMeC	Ml23SpVv[3Srb36gRZN{[Xl?	MnT0NlAwPTBizszN	MkjROFghcA>?		NELtcGtqdmS3Y3XzJINie3Cjc3WtN{Bi[3SrdnH0bY9v	NVrPeIMzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2OVY4PDZpPkK0OlU3PzR4PD;hQi=>
OVCAR-3	MlznR4VtdCCYaXHibYxqfHliQYPzZZk>	NVGzVItuOTBiwsXN	NVyydGxoOSCq		MYHlcohidmOnczDwZYNtcXSjeHXsMYlv\HWlZXSgc5ZiemmjbjDjZY5k\XJiY3XscEBl\WG2aNMg	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV{MEKyO{c,OjR3MkCyNlc9N2F-
OVCAR-3	NYTQVHRsSXCxcITvd4l{KEG\|c3H5	NHqwVowyOCEEtV2=	NXHrUmVkOSCq		NELRWG9xem:vb4Tld{Bx[WOuaYThfIVtNWmwZIXj[YQh[XCxcITvd4l{yqB?	NWDGfmFDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NlAzOjdpPkK0OVIxOjJ5PD;hQi=>
OVCAR-3	NVWwSJJuTnWwY4Tpc44hSXO\|YYm=	NF7P[oEyOCEEtV2=	NHjpXGUyKGh?		MY\lcohidmOnczDwZYNtcXSjeHXsMYlv\HWlZXSgZYN1cX[jdHnvckBw\iClYYPwZZNmNToEoB?=	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV{MEKyO{c,OjR3MkCyNlc9N2F-
OVCAR-3	MXzGeY5kfGmxbjDBd5NigQ>?	NH\iUJoyOCEEtV2=	NGPNXYIyKGh?		M1;kWoRwf25vcnXneYxifGW\|IF7GMe67SiCjY4TpeoF1cW:wIHnu[JVk\WRiYomgdIFkdGm2YYjlcC=>	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV{MEKyO{c,OjR3MkCyNlc9N2F-
OVCAR-3	MWXGeY5kfGmxbjDBd5NigQ>?	M1PUOFExKML3TR?=	NXzXTlRUOSCq		NYTKWm1bcW6qaXLpeJMheGGlbHn0ZZhmdC2rbnT1Z4VlKEGtdDDwbI9{eGixconsZZRqd25?	NWDHRXJJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NlAzOjdpPkK0OVIxOjJ5PD;hQi=>
CF33	MkHjSpVv[3Srb36gRZN{[Xl?	MVexNFAh\|ryP	NIfIc2wzPCCq		NIXkeopqdmS3Y3XzJIRwf26{ZXf1cIF1cW:wIH;mJGNQYC1{IIDyc5RmcW5iZYjwdoV{e2mxbh?=	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVyM{e4Nkc,OjR3MEO3PFI9N2F-
CF33	MlPpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NVPDflkxOTBvMUCwJO69VQ>?	MWGwMVQh\A>?		M1WyZYlvcGmkaYTzJINmdGxicILvcIln\XKjdHnvckBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=>	M161cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUCzO\|gzLz5{NEWwN\|c5OjxxYU6=
CF33	NYrtbpBDTnWwY4Tpc44hSXO\|YYm=	NUO3fVZDOTBvMUCwJO69VQ>?	NXeyXJdxPC1{NDDo		NVThW2xs\GWlcnXhd4V{KGOnbHzzJIlvKHSqZTDTJJBp[XOnIHHu[EBqdmO{ZXHz[ZMh[2WubIOgbY4hTzBxR{GgZZJz\XO2	NUPCO\|BMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NFM4QDJpPkK0OVA{Pzh{PD;hQi=>
LNCaP	NV65WmZkT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MoqyNk82NzFyIN88US=>	MXe5OkBp		NXvCZYtDcW6qaXLpeJMh[2WubDDndo94fGhic3nncolncWOjboTsfUBkd22kaX7l[EB4cXSqIHH0c5J3[XO2YYTpci=>	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ7Nkm3PEc,OjR{OU[5O\|g9N2F-
LNCaP	NHnoc4tCeG:ydH;zbZMhSXO\|YYm=	MWiyM\|UwOTBizszN	NX[we5JzQTZiaB?=		MnTobY5lfWOnczDhdI9xfG:\|aYOgZ49u[mmwZXSge4l1cCCjdH;yeoF{fGG2aX6=	NXezdJJ[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPVY6PzhpPkK0Nlk3QTd6PD;hQi=>
PC-3	NWGxbHVJT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NWHTXWNuOC13MDFOwG0>	NIHwNYw1QOLCiXi=		NYXpd4xzcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJ?	M3TjeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUK3PFgzLz5{NEGyO\|g5OjxxYU6=
PC-3	NF32U2RE\WyuIG\pZYJqdGm2eTDBd5NigQ>?	NF;0V40xNTFyMDFOwG0>	M3zrO\|czKGh?		MojP[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:\|ZTDk[ZBmdmSnboSgcYFvdmW{	NGTUcZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGyO\|g5Oid-MkSxNlc5QDJ:L3G+
HCA-7	M4jKSmZ2dmO2aX;uJIF{e2G7		MXS3NkBpenN?		M{PmZWlvcGmkaYTpc44hd2ZiSVytNYJmfGFvaX7keYNm\CCSR1WyJJBzd2S3Y4Tpc44hcW5iaIXtZY4hUEODLUegZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KEWLQTygSWM2OCB;IECuNFAyOTVizszNMi=>	NH\kfnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWxOlI5Oid-MkK1NVYzQDJ:L3G+
CHO	M2fUcmZ2dmO2aX;uJIF{e2G7				M4HkemlvKH[rdILvJIlvcGmkaYTvdpkheG:2ZX7jfUBi\2GrboP0JIh2dWGwIFPPXE0zKGmwIIP0ZYJtgSC2cnHud4Zm[3SnZDDjbIlv\XOnIHjhcZN1\XJib4\hdpkhMEOKTzmgZ4VtdHNuIFnDOVAhRSByLkCwNkDPxE1w	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODV5Nk[4OEc,OTB3N{[2PFQ9N2F-
CHO	MofiSpVv[3Srb36gZZN{[Xl?				MkTtTY5pcWKrdHnvckBw\iCSR1WtNkBxem:mdXP0bY9vKGmwIFPIU{Bk\WyuczDlfJBz\XO\|aX7nJIh2dWGwIFPPXE0zNCCLQ{WwJF0hOC5yMEKg{txONg>?	MkPKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB3N{[2PFUoRjFyNUe2Olg2RC:jPh?=
CHO	NHn5SWVHfW6ldHnvckBie3OjeR?=				NXPlXZpUUW5idnn0do8heG:2ZX7jfUBi\2GrboP0JIh2dWGwIGDyc5N1[WeuYX7kbY4hTy:KIIP5cpRp[XOnIEKgbY4hfHKjboPm[YN1\WRiQ1jPJINmdGy\|LDDJR\|UxKD1iMD6wNFIh\|ryPLh?=	NWO5[\|JQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCxPVc6PzBpPkGwNVk4QTdyPD;hQi=>
CHO	NI\1TVFHfW6ldHnvckBie3OjeR?=				NIXocGtKdmirYnn0c5J6KG:oIHj1cYFvKFC{b4P0ZYdt[W6maX6gS{9JKHO7boToZZNmKDJiZYjwdoV{e2WmIHnuJGNJVyClZXzsd{whUUN3MDC9JFAvODB{IN88UU4>	Mme3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ4NEO5OFIoRjF{NkSzPVQzRC:jPh?=
synovial cells	MnPmSpVv[3Srb36gZZN{[Xl?				MWfJcohq[mm2b4L5JIVn\mWldDDvckBxem:mdXP0bY9vKG:oIHP5Z4xwd3i7Z3XuZZNmNTJiKFPPXE0zMSCycn;zeIFodGGwZHnuJGUzKCiSR1WyLUBqdiCqdX3hckBKVDFvYnX0ZUB{fGmvdXzheIVlKHO7bn;2bYFtKGOnbHzzMEBKSzVyIE2gNE4xODd7IN88UU4>	Mk\JQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB6MkG3NVYoRjFyOEKxO\|E3RC:jPh?=
RAW264.7	NG\KfItHfW6ldHnvckBie3OjeR?=				NYm2XlM{UW6qaXLpeIlwdiCxZjDDU3guOiCrbjDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDMVHMucW6mdXPl[EBRT0V{IIDyc4R2[3Srb36geJJm[XSnZDDwdolweiC2bzDMVHMh[2ijbHzlcodmKGK7IHXufplu\SCrbX31co9ie3OjeTygTWM2OCB;IECuNFA5PyEQvF2u	MoLJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4NU[2OlIoRjJ2NkW2OlYzRC:jPh?=
HEK293	MoXaSpVv[3Srb36gZZN{[Xl?				MkDuTY5pcWKrdHnvckBw\iClbH;u[YQhW3S7bH;wbI9z[SCyaYP0bYxt[XSjIHHsdIhiNUODIHX4dJJme3OnZDDpckBpfW2jbjDISWszQTNiY3XscJMh[nlic4TvdJBm\C2obH;3JGNQOiCjc4PhfUwhU2liPTCwMlA{PDJizszNMi=>	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV{MEW3O{c,OTl3MkC1O\|c9N2F-
SF9	M1TVVmZ2dmO2aX;uJIF{e2G7				M3PCeWlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQdo9{fGGpbHHu[IlvKEdxSDDzfY51cGG\|ZTCyJIV5eHKnc4Pl[EBqdiC\|Zj25JINmdGy\|IHnu[oVkfGWmIIfpeIgh[mGldXzveolzfXNuIFnDOVAhRSByLkCzOkDPxE1w	NGnFNpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNE[5PFE6OCd-MUS2PVgyQTB:L3G+
SF9	M2Tkb2Z2dmO2aX;uJIF{e2G7				NF\memxKdiC4aYTyc{BqdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhcHWvYX6gdoVkd22kaX7hcpQheHKxc4Th[4xidmSrbjDHM2ghe3mwdHjhd4UhOiCneIDy[ZN{\WRiaX6gd4YuQSClZXzsd{Bqdm[nY4Tl[EB4cXSqIHLhZ5Vtd3[rcoXzMEBKSzVyIE2gNE4xOzZizszNMi=>	M2\aXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MEK2NFUxLz5zNUCyOlA2ODxxYU6=
CHO	NH;2fpVHfW6ldHnvckBie3OjeR?=				NFzUOoNKdmirYnn0bY9vKG:oIFPPXFIh\XiycnXzd4VlKGmwIFPIU{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHHyZYNpcWSxbnnjJIFkcWRvc4TpcZVt[XSnZDDQS2UzKHC{b3T1Z5Rqd25iYomg[Y57gW2nIHntcZVvd2G\|c3H5MEBKSzVyIE2gNE4xOzZizszNMi=>	NVHIfo1IRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW1OlYzQTBpPkG1OVY3OjlyPD;hQi=>
insect cells	MWHGeY5kfGmxbjDhd5NigQ>?		MkG1NUBucW5?		M3;zSWlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiQ1;YNkBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzJJV{cW6pIHHyZYNpcWSxbnnjJIFkcWRiYYOgd5Vje3S{YYTlJIlv[3WkYYTl[EBnd3JiMTDtbY4heHKrb4KgeI8he3Wkc4TyZZRmKGGmZHn0bY9vKG2nYYP1doVlKG[xcjCyOUB{\WO\|IHL5JHROWERvYnHz[YQh[2i{b33v[4VvcWNiYYPzZZktKEmFNUCgQUAxNjB2MjFOwG0v	NWLxdYZGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNVAzPzBpPkKzNFExOjdyPD;hQi=>
HNSCC 1483	MXHGeY5kfGmxbjDhd5NigQ>?		M3zEZ\|MxKG2rboO=		NUDmVppnUW6qaXLpeIlwdiCxZjDDU3guOiCrbjDoeY1idiCKTmPDR{AyPDh\|IHPlcIx{KHW\|aX7nJHsyPEOfIHHyZYNpcWSxbnnjJIFkcWRiYYOgd5Vje3S{YYTlJJBz\WmwY4XiZZRm\CCob4KgN\|AhdWmwczDi[YZwemVic4Xid5Rz[XSnIHHk[Il1cW:wIH3lZZN2emWmIHHmeIVzKDNyIH3pcpMtKEmFNUCgQUAxNjB3NDFOwG0v	NW\yfXRlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlM5QTRpPkKyNlY{QDl2PD;hQi=>
J774	NF;4XldHfW6ldHnvckBie3OjeR?=				NXLxd2dKUW6qaXLpeIlwdiCxZjDDU3gzKGmwIFzQV{1{fGmvdXzheIVlKEp5N{SgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCSR1WyJIxmfmWuczDifUBz[WSrb3ntcZVvd2G\|c3H5MEBKSzVyIE2gNE4xPiEQvF2u	NHT2Umc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mxOVg2PCd-MUe5NVU5PTR:L3G+
J774	NYDuUpJnTnWwY4Tpc44h[XO\|YYm=		MnnWNVUhdWmwcx?=		MUPJcohq[mm2aX;uJI9nKEORWEKgbY4hVFCVLYP0bY12dGG2ZXSgcY92e2ViSke3OEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGDHSVIheHKxZIXjeIlwdiCjZoTldkAyPSCvaX7zJIJ6KHKjZHnvbY1ufW6xYYPzZZktKEmFNUCgQUAxNjB4IN88UU4>	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDd3Mkm1O{c,OTh5NUK5OVc9N2F-
J774	NFW0[2ZHfW6ldHnvckBie3OjeR?=		MUCyOEBpenN?		MlXUTY5pcWKrdHnvckBw\iCFT2itNk1u\WSrYYTl[EBRT0V{IIDyc4R2[3Srb36gbY4hVFCVLYP0bY12dGG2ZXSgcY92e2ViSke3OEBk\WyuczDh[pRmeiB{NDDodpMh[nlicnHkbY9qdW23bn;hd5NigSxiSVO1NEA:KDBwME[xJO69VS5?	NHjx[ZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm5NlE4Pid-MkG5PVIyPzZ:L3G+
J774	MmHMSpVv[3Srb36gZZN{[Xl?				NFfub3RKdmirYnn0bY9vKG:oIFPPXFIhcW5ibX;1d4UhUjd5NDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIGDHSVIheHKxZIXjeIlwdiCkeTDyZYRqd2mvbYXuc4F{e2G7LDDJR\|UxKD1iMD6wOlEh\|ryPLh?=	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ6MES0OEc,OjN4OEC0OFQ9N2F-
SF9	MWnGeY5kfGmxbjDhd5NigQ>?				MYHJcohq[mm2aX;uJI9nKGi3bXHuJGNQYDJiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{NWmwZnXjeIVlKFOIOTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGG{YXPobYRwdmmlIHHjbYQue3SrbYXsZZRm\CCSR1WyJJBzd2S3Y4Tpc44hfHKnYYTl[EAyKGi{IHLl[o9z\SCjcnHjbIlld26rYzDhZ4llKGOqYXzs[Y5o\SCkeTDlcpp6dWViaX3teY5w[XO\|YYmsJGlEPTBiPTCwMlA3OyEQvF2u	NEHHfXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWyNFU4Oyd-MUm1NlA2PzN:L3G+
COS	NH20R3NHfW6ldHnvckBie3OjeR?=				MmfFTY5pcWKrdHnvckBw\iCqdX3hckBkgWOub3;4fYdmdmG\|ZT2yJIV5eHKnc4Pl[EBqdiCFT2OgZ4VtdHNuIFnDOVAhRSByLkC2PEDPxE1w	MlfLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV2NUSyOFIoRjF3NEW0NlQzRC:jPh?=
COS	NFvyb5NHfW6ldHnvckBie3OjeR?=				M2K0OWlvcGmkaYTpc44hd2ZiaIXtZY4hS0:[MjDlfJBz\XO\|ZXSgbY4h[W[{aXPhckBoemWnbjDtc45s\XliQ1;TJINmdGy\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiYYLhZ4hq\G:waXOgZYNq\C2\|dHnteYxifGWmIGDHSVIheHKxZIXjeIlwdiC2cnXheIVlKDFiaIKgZoVnd3KnIHHyZYNpcWSxbnnjJIFkcWRiY3jhcIxmdmenIHL5JIVvgnmvZTDpcY12dm:jc4PhfUwhUUN3MDC9JFAvODZ6IN88UU4>	MkLLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3MkC1O\|MoRjF7NUKwOVc{RC:jPh?=
143982	MV7GeY5kfGmxbjDhd5NigQ>?				M33Z[GlvKH[rdILvJIlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQdo9{fGGpbHHu[IlvKEdxSDDzfY51cGG\|ZTCyJEhEV1hvMjmgbY4hOTR\|OUiyJINmdGy\|LDDJR\|UxKD1iMD6wO\|kh\|ryPLh?=	M4j2bVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OEe3OVg1Lz5zMki3O\|U5PDxxYU6=
J774	NGn4WJpHfW6ldHnvckBie3OjeR?=				MkmzTY4hfmm2cn:gbY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JHBzd3O2YXfsZY5lcW5iRz;IJJN6dnSqYYPlJFIhcW5ibYXybY5mKEp5N{SgZ4VtdHNuIFnDOVAhRSByLkC3PUDPxE1w	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTh3N{G0PUc,OTV6NUexOFk9N2F-
J774	MnPPSpVv[3Srb36gZZN{[Xl?				MXTJcohq[mm2aX;uJI9nKEORWEKt[IVx\W6mZX70JHBITTJicILv[JVkfGmxbjDpckBNWFNvc4TpcZVt[XSnZDDtc5V{\SCMN{e0JINmdGy\|IHL5JHJKSSxiSVO1NEA:KDBwMEe5JO69VS5?	MmXpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7NUe5N\|EoRjF7OUW3PVMyRC:jPh?=
osteosarcoma cells	NWPa[5dmTnWwY4Tpc44h[XO\|YYm=				MoL2TY5pcWKrdHnvckBw\iCScn;zeIFodGGwZHnuJGcwUCC\|eX70bIF{\SB{IHnuJFE1Oy57OD6yJIZzd21iaIXtZY4hd3O2ZX;zZZJkd22jIHPlcIx{NCCLQ{WwJF0hOC5yODFOwG0v	M3GyVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzNE[yPVc3Lz5zMUS2Nlk4PjxxYU6=
SF9	Mo\HSpVv[3Srb36gZZN{[Xl?				MWTJcohq[mm2aX;uJI9nKGi3bXHuJGNQYDJiZYjwdoV{e2WmIHnuJJNnQSClZXzsd{whUUN3MDC9JFAvODhizszNMi=>	NUTDN\|hORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[yOVI6OTdpPkG2NlUzQTF5PD;hQi=>
RAW264.7	NUnlOHdKSW62aXnu[oxidW2jdH;yfUBie3OjeR?=				M3zKdWFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDQS2UzKHC{b3T1Z5Rqd25uIFnDOVAhRSByLkGg{txONg>?	M1TCSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MECwPVQ5Lz5{MkCwNFk1QDxxYU6=
RAW264.7	NFrme4VHfW6ldHnvckBie3OjeR?=				MUnJcohq[mm2aX;uJI9nKEORWD2yJIlvKG2xdYPlJHJCXzJ4ND63JINmdGy\|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKEySUz3pcoR2[2WmIGDHSVIheHKxZIXjeIlwdixiSVO1NEA:KDBwMUKg{txONg>?	M3HsZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|M{WzO\|QyLz5{M{O1N\|c1OTxxYU6=
Sf21	NFLnNJNHfW6ldHnvckBie3OjeR?=				MUDJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKE5vdHXycYlv[WxiSHnzMZRi\2enZDDDU3gzKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EBU\jJzIHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBxem:\|dHHncIFv\GmwIIDyc4R2[3Srb36gZpkh\W68eX3lJIludXWwb3Hzd4F6NCCLQ{WwJF0hOC5zNTFOwG0v	NV\jVmJGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmzO\|MzPzFpPkK5N\|c{OjdzPD;hQi=>
Sf21	MVrGeY5kfGmxbjDhd5NigQ>?		MmHnOUBucW6\|		M{iyUmlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgUk11\XKvaX7hcEBJcXN4LYTh[4dm\CCqdX3hckBEV1hvMjDlfJBz\XO\|ZXSgbY4hSmGldXzveolzfXNiaX7m[YN1\WRiU3[yNUBk\WyuczD1d4lv\yCjcnHjbIlld26rYzDhZ4llKGG\|IIP1ZpN1emG2ZTDwdoVqdmO3YnH0[YQh\m:{IEWgcYlveyCob3zsc5dm\CCkeTDzeYJ{fHKjdHWgZYRlcXSrb36gcYVie3W{ZXSgZYZ1\XJiMTDodkwhUUN3MDC9JFAvOjZizszNMi=>	M2LEcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkmzO\|k2Lz5\|MEK5N\|c6PTxxYU6=
MC9	MnHJSpVv[3Srb36gZZN{[Xl?				NEXhWmRKdmirYnn0bY9vKG:oIGDHSlJieGijIIDyc4R2[3Srb36gbY4h[XKjY3jp[I9vcWNiYXPp[E1{fGmvdXzheIVlKG2xdYPlJG1EQSClZXzsd{whUUN3MDC9JFAvOyEQvF2u	M2f3clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEm4NVUxLz5zOES5PFE2ODxxYU6=
MC9	NICybJhHfW6ldHnvckBie3OjeR?=				M3rOUGlvcGmkaYTpc44hd2ZiUFfGNoFteGijIIDyc4R2[3Srb36gbY4hdW:3c3WgUWM6KGOnbHzzMEBKSzVyIE2gNE41KM7:TT6=	M4m4RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEm4NVUxLz5zOES5PFE2ODxxYU6=
293E	M4PNT2Z2dmO2aX;uJIF{e2G7				MlPyTY5pcWKrdHnvckBw\iCqdX3hckBucWO{b4PvcYFtKFCJRWOxJIV5eHKnc4Pl[EBqdiB{OUPFJINmdGy\|IHL5JGxEN02VL13TJIFv[Wy7c3nzMEBKSzVyIE2gNE41OjVizszNMi=>	NWrifVVNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[2OVMyQDBpPkK2OlU{OThyPD;hQi=>
SF9	MWfGeY5kfGmxbjDhd5NigQ>?		M3fxeFE2KG2rboO=		NHe5ZnlKdmirYnn0bY9vKG:oIH\1cIwhdGWwZ4ToJJJm[2:vYnnuZY51KGi3bXHuJG4ufGW{bXnuZYwhT1OWLYTh[4dm\CCKRFHDOkBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOgbY5n\WO2ZXSgd4Y6KGOnbHzzJJV{cW6pIFLvZ{1NgXNvKFHjLU1CVUNiYYOgd5Vje3S{YYTlJJBz\WmwY4XiZZRm\CCob4KgNVUhdWmwczDmc4xtd3enZDDifUB{fWK\|dILheIUh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgOlAhdWmwczDifUBndHWxcnXzZ4Vv[2ViYYPzZZktKEmFNUCgQUAxNjZ2MzFOwG0v	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTVyMEGzNEc,Ojl3MECxN\|A9N2F-
HT-29	NUjkU2xSS3m2b4TvfIlkcXS7IHHzd4F6		M4nFZVQ5KGi{cx?=		M2TPSmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEORWD2yJJBwe2m2aY\lJIh2dWGwIFjUMVI6KGOnbHzzJJRz[W6\|ZnXjeIVlKHerdHigR2Q1PHZ4c3jSUmEh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwNzFOwG0v	NULhUYFsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPVU4QDdpPkK0Nlk2Pzh5PD;hQi=>
SF9	MlXwSpVv[3Srb36gZZN{[Xl?		M1m1flE2KG2rboO=		M1\TOmlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgUIVqe2ivYX7pZUBld26xdnHubUBkcGGpYYPpJIJmfGFvY3HyZo9vcWNiYX7ofYRz[XOnIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2eyCrbn\lZ5Rm\CCrboPlZ5QhW2Z7IHPlcIx{KGmwY4XiZZRm\CCob4KgNVUhdWmwczDwdolweiC2bzD0[ZN1cW6pIHL5JJN1d3CyZXSg[oxwfyCFT{KgbJllemG\|ZTDhd5NigSxiS3mgQUAxNjdyNTFOwG0v	MkDZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7N{e5OlAoRjJ\|OUe3PVYxRC:jPh?=
HCA-7	M3WzNWN6fG:2b4jpZ4l1gSCjc4PhfS=>		MVq0PEBpenN?		Mn;xR5l1d3SxeHnjbZR6KGGpYXnud5QhS0:[LUKgdI9{cXSrdnWgbJVu[W5iSFPBMVch[2WubIOgeJJidnOoZXP0[YQhf2m2aDDDSFQ1fj[\|aGLORUBie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC55NTFOwG0v	NEjyTo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK5OVc5Pyd-MkSyPVU4QDd:L3G+
HepG2	NHS2OXRCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M3vMc\|Q5KGi{cx?=		M3rneWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHXwS\|Ih[2WubIOgbY5kfWKjdHXkJIZweiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxKD1iMD63OkDPxE1w	MlPsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ\|NE[zOlcoRjJ4M{S2N\|Y4RC:jPh?=
SF9	MV3GeY5kfGmxbjDhd5NigQ>?		MYSxOUBucW6\|		MYXJcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIILlZ49u[mmwYX70JIh2dWGwIFOteIVzdWmwYXygSmxCTy:KaYOteIFo\2WmIFjERWMyKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EB{\jliY3XscJMhfXOrbnegRo9kNUy7cz2oRYMqNUGPQzDhd{B{fWK\|dILheIUheHKnaX7jeYJifGWmIH\vdkAyPSCvaX7zJIZwdGyxd3XkJIJ6KHO3YoP0doF1\SCjZHTpeIlwdiCvZXHzeZJm\CCjZoTldkA3OCCvaX7zJIJ6KG[udX;y[ZNk\W6lZTDhd5MtKEmFNUCgQUAyNjF6NTFOwG0v	NFj5emQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWwNFE{OCd-Mkm1NFAyOzB:L3G+
RAW264.7	NUTJ[ox5SW62aXnu[oxidW2jdH;yfUBie3OjeR?=		MlTTNVghcHK\|		M3zjfWFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDUUmYu[WyyaHGgd4VkemW2aX;uJIFnfGW{IEG4JIhzeyCkeTDzZY5lf2mlaDDFUGlUSSxiSVO1NEA:KDFwMkig{txONg>?	M1:5NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Nke5OFQyLz5{NE[3PVQ1OTxxYU6=
APC10.1	NHf1bJFEgXSxdH;4bYNqfHliYYPzZZk>		MoDkOFghcHK\|		NEfWeYpEgXSxdH;4bYNqfHliYXfhbY5{fCCFT2itNkBxd3OrdHn2[UBud3W\|ZTDBVGMyOC5zIHPlcIx{KHS{YX7z[oVkfGWmIIfpeIghS0R2NI[2d4hTVkFib4\ldoV5eHKnc4PpcochUEGVLUKgZZN{\XO\|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgOFghcHK\|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFEvOyEQvF2u	NXThT482RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPVU4QDdpPkK0Nlk2Pzh5PD;hQi=>
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HT-29	MWDDfZRwfG:6aXPpeJkh[XO\|YYm=		NF7sUGoyQCCqcoO=		NIrTdlBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJXC1{OTDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG\|ZTDpckBk\WyuIIP1dpZqfmGuIHHmeIVzKDF6IHjyd{BjgSCPVGOgZZN{[XluIFnDOVAhRSB\|IN88UU4>	Mlz4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MUe3NlEoRjJ\|NUG3O\|IyRC:jPh?=
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J774	MmfuSpVv[3Srb36gZZN{[Xl?		M3TOblE2KG2rboO=		Mk\6TY5pcWKrdHnvckBw\iCFT2itNU1u\WSrYYTl[EBRT0V{IIDyc4R2[3Srb36gbY4h[XKjY3jp[I9vcWNiYXPp[E1{fGmvdXzheIVlKG2xdYPlJGo4PzRiY3XscJMhcW6ldXLheIVlKG[xcjCxOUBucW6\|IIDybY9zKHSxIHHyZYNpcWSxbnnjJIFkcWRvY3jhcIxmdmenIHL5JJJi\GmxaX3teY5w[XO\|YYmsJGlEPTBiPTCzMlg1KM7:TT6=	MmPPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7OUKxO\|YoRjJzOUmyNVc3RC:jPh?=
J774	Mm[3SpVv[3Srb36gZZN{[Xl?		M3HIfVE2KG2rboO=		NHfSVJlKdmirYnn0bY9vKG:oIFPPXFEhcW5ibX;1d4UhUjd5NDDj[YxteyC3c3nu[{BiemGlaHnkc45q[yCjY3nkJIF{KHO3YoP0doF1\SCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFCJRUKgdJJw\HWldHnvckBqdmO3YnH0[YQh\m:{IEG1JI1qdnNicILpc5IhfG9ic4Xid5Rz[XSnIHHk[Il1cW:wIH3lZZN2emWmIHHmeIVzKDNyIH3pcpMh[nlicnHkbY9qdW23bn;hd5NigSxiSVO1NEA:KDNwOESg{txONg>?	NXnkOVJHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFA1PDRpPkKzOlgxPDR2PD;hQi=>
APC10.1	NXLHe\|c3S3m2b4TvfIlkcXS7IHHzd4F6		NVXxUGFxOThiaILz		MV3DfZRwfG:6aXPpeJkh[WejaX7zeEBud3W\|ZTDBVGMyOC5zIHPlcIx{KGG\|c3Xzd4VlKGG\|IHTlZ5Jm[XOnIHnuJINmdGxic4Xyeol3[WxiYX\0[ZIhOThiaILzJIJ6KE2WUzDhd5NigSxiSVO1NEA:KDRwMjFOwG0v	Mnz3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MUe3NlEoRjJ\|NUG3O\|IyRC:jPh?=
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J774	M2DYOWZ2dmO2aX;uJIF{e2G7				MWjJckB3cXS{bzDpcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgVJJwe3SjZ3zhcoRqdiCJL1igd5lvfGijc3WgNUBqdiCvdYLpcoUhUjd5NDDj[YxteyxiSVO1NEA:KDVwMTFOwG0v	M{[yOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OEW3NVQ6Lz5zNUi1O\|E1QTxxYU6=
J774	NVrOT5VqTnWwY4Tpc44h[XO\|YYm=				MU\Jcohq[mm2aX;uJI9nKEORWEGt[IVx\W6mZX70JHBITTJicILv[JVkfGmxbjDpckBNWFNvc4TpcZVt[XSnZDDtc5V{\SCMN{e0JINmdGy\|IHL5JHJKSSxiSVO1NEA:KDVwMTFOwG0v	M3zHUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OUW3PVMyLz5zOUm1O\|k{OTxxYU6=
HT-29	MnjYR5l1d3SxeHnjbZR6KGG\|c3H5				MlnER5l1d3SxeHnjbZR6KGGpYXnud5QhS0:[LUKgdI9{cXSrdnWgbJVu[W5iSGStNlkh[2WubIOgZZN{\XO\|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[nliQ3XscHRqfGW{LUm2JGFSfWWxdYOgZZN{[XluIFnDOVAhRSB3Lkeg{txONg>?	NXrW[3JjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPVU4QDdpPkK0Nlk2Pzh5PD;hQi=>
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HeLa	NGHFS3hCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M3;lflQ5KGi{cx?=		MnvNRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCKZVzhJINmdGy\|IHnuZ5Vj[XSnZDDmc5IhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDdwNUWg{txONg>?	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjN2NkO2O{c,OjZ\|NE[zOlc9N2F-
HeLa	NFW1NpZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		Mn;KNlQhcHK\|		NU\5R2JISW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTBiPTC3Mlc6KM7:TT6=	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzN2OUOzNUc,Ojd\|NEmzN\|E9N2F-
HepG2	NFvxOoVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NFPn[mszPCCqcoO=		NUfjPYp5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNVAvODNizszNMi=>	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzN2OUOzNUc,Ojd\|NEmzN\|E9N2F-
APC10.1	MnrOR5l1d3SxeHnjbZR6KGG\|c3H5				NWTBNJRqS3m2b4TvfIlkcXS7IHHnZYlve3RiQ1;YMVIheG:\|aYTpeoUhdW:3c3WgRXBEOTBwMTDj[YxteyCxdnXy[ZhxemW\|c3nu[{BJSVNvMjDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBjgSCFZXzsWIl1\XJvOU[gRXF2\W:3czDhd5NigSxiSVO1NEA:KDF\|LkSg{txONg>?	M1W4[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mkm1O\|g4Lz5{NEK5OVc5PzxxYU6=
CHO	NIjNU2JHfW6ldHnvckBie3OjeR?=				MoHPTY5pcWKrdHnvckBw\iCFT2ixJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCjcnHjbIlld26rYzDhZ4llNXO2aX31cIF1\WRiUFfFNkBxem:mdXP0bY9vKGK7IHXufplu\SCrbX31co9ie3OjeTygTWM2OCB;IEGzMlUh\|ryPLh?=	M3TUOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NU[2NlkxLz5zNUW2OlI6ODxxYU6=
B16F10	MkHFRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NGC4cY0zPCCqcoO=		NIPqSHpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIFKxOmYyOCClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNVQvOzZizszNMi=>	M4XWXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5M{S5N\|MyLz5{N{O0PVM{OTxxYU6=
A549	MkLHRY51cXS3bX;yJIF{e2G7		NYDw[Jo{PDhiaILz		M3LT[2FvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36gZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEG1MlYh\|ryPLh?=	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN3M{e0NUc,OjN\|NUO3OFE9N2F-
A549	NXy3OWN[SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M2TD[VI1KGi{cx?=		MX7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF3NEmgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDF3Lk[0JO69VS5?	NV3C[ZVVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkezOFk{OzFpPkK3N\|Q6OzNzPD;hQi=>
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A549	M3zzeWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MkjSOFghcHK\|		MnrmRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCDNUS5JINmdGy\|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzNj6wPEDPxE1w	NUnPeop7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwN\|E3PTJpPkOwNFMyPjV{PD;hQi=>
NCI60	MVvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NFuwUZI1QCCqcoO=		NFTEO3BIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBPS0l4MDDj[YxteyCjZoTldkA1QCCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTygS2k2OCB;IEG3MlUh\|ryPLh?=	NVf1Z2JtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCzPFc5OTVpPkKwN\|g4QDF3PD;hQi=>
HCA-7	M2PIPWZ2dmO2aX;uJIF{e2G7		MkGxNVghcHK\|		MX;Jcohq[mm2aX;uJI9nKGi7YXz1do9v[W5vaX7keYNm\CCFRES0JIFvfGmpZX6geoFzcWGwdDDlfI9vKDZiYXP0bZZqfHliaX6gbJVu[W5iSFPBMVch[2WubIOg[ZhxemW\|c3nu[{BJ[XN{IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGOnbHygd5Vzfmm4YXygZYZ1\XJiMUigbJJ{KGK7IF3UV{Bie3OjeTygTWM2OCB;IEG3MlUh\|ryPLh?=	NWHKOGhFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NVc4OjFpPkKzOVE4PzJzPD;hQi=>
HT-29	NH3WTXpHfW6ldHnvckBie3OjeR?=		Mn3VNVghcHK\|		MYLJcohq[mm2aX;uJI9nKGi7YXz1do9v[W5vaX7keYNm\CCFRES0JIFvfGmpZX6geoFzcWGwdDDlfI9vKDZiYXP0bZZqfHliaX6gbJVu[W5iSGStNlkh[2WubIOg[ZhxemW\|c3nu[{BJ[XN{IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGOnbHygd5Vzfmm4YXygZYZ1\XJiMUigbJJ{KGK7IF3UV{Bie3OjeTygTWM2OCB;IEG4MlMh\|ryPLh?=	M373XFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NUG3O\|IyLz5{M{WxO\|czOTxxYU6=
platelet	NGfTU2pHfW6ldHnvckBie3OjeR?=				MXTJcohq[mm2b4L5JIVn\mWldDDvckBxem:mdXP0bY9vKG:oIHP5Z4xwd3i7Z3XuZZNmNTFiKFPPXE0yMSC2aILvcYJwgGGwZTDCNkApXFiEMjmgbY4hcHWvYX6gdIxifGWuZYSgZ4VtdHNuIFnDOVAhRSBzOTFOwG0v	NULOXHhORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC4NlE4OTZpPkGwPFIyPzF4PD;hQi=>
B16F10	MYnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MXO0PEBpenN?		M4LxWmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViQkG2SlExKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTBiPTCxPU4yPSEQvF2u	MnvCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByM{G2OVIoRjNyMEOxOlUzRC:jPh?=
MDA231	M4LFS2FvfGmycn;sbYZmemG2aY\lJIF{e2G7				MmntRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPRFGyN\|Eh[2WubIOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOjJwN{mg{txONg>?	NHq0UXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEKxOlg1QCd-M{CyNVY5PDh:L3G+
MCF7	MmTzRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MmTEOFghcHK\|		NF7JSVJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEK2Mlc5KM7:TT6=	NUnNbWNDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwN\|E3PTJpPkOwNFMyPjV{PD;hQi=>
HCT116	MnvqRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?				Mm[xRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCKQ2SxNVYh[2WubIOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOjlwNUSg{txONg>?	NVj2fJpwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyNVY5PDhpPkOwNlE3QDR6PD;hQi=>
MCF7	NW[0VmlNSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=				NHjhNZVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOzFwMkig{txONg>?	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJzNki0PEc,OzB{MU[4OFg9N2F-
LNCAP	NHPIWYVEgXSxdH;4bYNqfHliYYPzZZk>		MUK3NkBpenN?		MYrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMUmNCWCClZXzsd{Bie3Onc4Pl[EBieyClZXzsJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTBiPTCzNk43KM7:TT6=	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB3N{SwO{c,OjhyNUe0NFc9N2F-
BL21(DE3)	MlXBSpVv[3Srb36gZZN{[Xl?		NV\yTmh3OTVibXnudy=>		MlLXTY5pcWKrdHnvckBw\iCJU2SteIFo\2WmIF3hcIF{e2W8aXGg[4xw[m:\|YTDBWGNEKDl4OEC3M2NDWyB5OU[2JG1INUODIHX4dJJme3OnZDDpckBGe2OqZYLpZ4hq[SClb3zpJGJNOjFqRFWzLUBk\WyuczDwdoVqdmO3YnH0[YQh\m:{IEG1JI1qdnNibXXhd5Vz\WRiZn;yJFExKHSxIEGwNEB{\WNiYomgd5RweHCnZD3mcI94KG2ndHjv[EwhU2liPTCzOE45KM7:TT6=	NF7Pc4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOFI{QSd-MkK0NlQzOzl:L3G+
HeLa	MojXRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?				MVfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEinTHGgZ4VtdHNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gN\|YvODhizszNMi=>	NWHGeZBRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyNVY5PDhpPkOwNlE3QDR6PD;hQi=>
MDA-MB-231	M336Z2N6fG:2b4jpZ4l1gSCjc4PhfS=>		MUm0PEBpenN?		M2jkVmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN\|Eh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UV{Bie3OjeTygTWM2OCB;IESwJO69VS5?	MoHNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5OEC5OlEoRjJ{N{iwPVYyRC:jPh?=
MCF7	MWnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		M2HSWVQ5KGi{cx?=		NVHJNo04SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[2:ub4LpcYV1emmlIHHzd4F6NCCLQ{WwJF0hPDBwODFOwG0v	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjByMEm0PEc,OjJyMEC5OFg9N2F-
MCF7	NIr1V\|BCdnSrdIXtc5Ih[XO\|YYm=		MnjhOFghcHK\|		MX;BcpRqfHWvb4KgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPQ1[3JINmdGy\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:wIHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB2MD64JO69VS5?	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN3M{e0NUc,OjN\|NUO3OFE9N2F-
Caco2	MU\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?				NXewT44xSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDDZYNwOiClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB2Mj63OEDPxE1w	NHG4Xms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEKxOlg1QCd-M{CyNVY5PDh:L3G+
HCT116	NV;pO\|Q6SW62aXPhcoNmeiCjc4PhfS=>		MoXOO\|IhcHK\|		MoPIRY51cWOjbnPldkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gOFMvOyEQvF2u	NH7W[5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MU[3PFk4OSd-MkG2O\|g6PzF:L3G+
L1210	NYPINph5S3m2b4TvfIlkcXS7IHHzd4F6		M1nPelczKGi{cx?=		NHPkU3ZEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBNOTJzMDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hPDRizszNMi=>	NFnTT209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES1NVM6Pyd-MkC0OVE{QTd:L3G+
HT-29	NYqyXZFES3m2b4TvfIlkcXS7IHHzd4F6				NWK0cGRiS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUFRvMkmgZ4VtdHNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gOFUvPSEQvF2u	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODhyNEG5O{c,OjB6MESxPVc9N2F-
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Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	Fibronectin / Vimentin / N-cadherin / E-cadherin ; COX-2 ; pAKT / AKT / PPARγ / PTEN	28978083 29515134 24721996
Immunofluorescence	PPARγ / PTEN	24721996
Growth inhibition assay	Cell viability	28521485

In vivo

Celecoxib exhibits a potent, oral anti-inflammatory activity. Celecoxib reduces acute inflammation in the carrageenan edema assay and chronic inflammation in the adjuvant arthritis model with ED50 of 7.1 mg/kg and 0.37 mg/kg/day, respectively. In addition, Celecoxib also exhibits analgesic activity in the Hargreaves hyperalgesia model with ED50 of 34.5 mg/kg. Besides, Celecoxib produces no acute GI toxicity in rats at doses up to 200 mg/kg and no chronic GI toxicity in rats at doses up to 600 mg/kg/day over 10 days. ^[1] In a C3Hf/KamLaw female mouse model, Celecoxib increases median survival time of 105 days (range, 79-145 days) after 13.5 Gy local thoracic irradiation (LTI) alone to 142 days (range, 94-155 days). ^[3]

Protocol (from reference)

Kinase Assay:^[1]	COX enzyme assay in vitro: Expression of COX protein in insect cells is determined by assessing PG-synthetic capability in homogenates from cells incubated for 3 days with COX-1 or COX-2 baculovirus. Cells expressing COX-1 or COX-2 are homogenized and incubated with arachidonic acid (10 μM). COX activity is determined by monitoring PG production. No COX activity is detected in mock-infected Sf9 cells. Celecoxib are preincubated with crude 1% CHAPS homogenates (2-10 μg of protein) for 10 minutes before addition of arachidonic acid. PGE2 formed is detected by ELISA after 10 minute incubation.
Cell Research:^[2]	Cell lines: HNE1 and CNE1-LMP1 Concentrations: 0-75 μM Incubation Time: 48 hours Method: The antiproliferative effect of Celecoxib on NPC cells is assessed using an MTT assay. Cells are seeded into 96-well plates and allowed to attach for 24 hours. The cells are then treated with increasing concentrations of Celecoxib (0 to 75 μM) dissolved in DMSO (final concentration ≤0.1%) and incubated for up to 48 hours. After the incubation, 20 μL of MTT dye (5 mg/mL) are added to each well and cells are incubated at 37 °C for 4 hours. After removing the supernatants, the crystals are dissolved in DMSO and the absorbance is measured at 490 nm. The half-maximal inhibitory concentration (IC50) values and the 95% confidence intervals are calculated using probit regression using SPSS 15.0 software. The experiment is performed in triplicate and repeated at least three times. (Only for Reference)
Animal Research:^[1]	Animal Models: A 0.1 mL aliquot of a 1% solution of carrageenan in 0.9% sterile saline or 1 mg of Mycobacterium butyricum in 50 μL of mineral oil is administered to the right hind foot pad of male Sprague−Dawley rats. Dosages: ≤200 mg/kg Administration: Administered via p.o. (Only for Reference)

References

Solubility (25°C)

In vitro	DMSO	76 mg/mL (199.28 mM)
	Water	Insoluble
	Ethanol	'33 mg/mL
In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): 2% DMSO+30% PEG 300+5% Tween 80+ddH2O For best results, use promptly after mixing. Click to purchase: PEG300 Tween 80	5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	381.37
Formula	C₁₇H₁₄F₃N₃O₂S
CAS No.	169590-42-5
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CC1=CC=C(C=C1)C2=CC(=NN2C3=CC=C(C=C3)S(=O)(=O)N)C(F)(F)F

Download Celecoxib (SC 58635) SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

Clinical Trial Information

NCT Number	Recruitment	Interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT01344200	Not yet recruiting	Drug: Celecoxib\|Drug: Placebo	Pharmacokinetics of Celecoxib in Children	Children''s Hospital of Eastern Ontario	January 2022	Phase 2
NCT04526197	Completed	Drug: ALXN1840\|Drug: Celecoxib	Wilson Disease	Alexion Pharmaceuticals	July 8 2020	Phase 1
NCT04447261	Completed	Drug: BI 1356225\|Drug: Placebo	Obesity	Boehringer Ingelheim	June 30 2020	Phase 1
NCT03896113	Recruiting	Drug: Celecoxib 200mg capsule	Endometrium Cancer	Cliniques universitaires Saint-Luc- Université Catholique de Louvain	November 13 2019	Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Frequently Asked Questions

Question 1:
I am doing an animal experiment with mice and planning to administrate S1261 via oral gavage. what are your recommendations for diluting the compound?

Answer:
For oral administration, Celecoxib can be dissolved in vehicle one: "0.5% methyl cellulose and 0.025% Tween-20", or the vehicle 2 "2% DMSO/30% PEG 300/5% Tween 80/ ddH2O".

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