Asaraldehyde

Catalog No.S2531 Synonyms: Asaronaldehyde

For research use only.

Asaraldehyde (Asaronaldehyde) is a natural COX-2 inhibitor, exhibiting 17-fold selectivity over COX-1.

Asaraldehyde Chemical Structure

CAS No. 4460-86-0

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Biological Activity

Description Asaraldehyde (Asaronaldehyde) is a natural COX-2 inhibitor, exhibiting 17-fold selectivity over COX-1.
Targets
COX-2 [2]
In vitro

Asaraldehyde is found to be the most abundant constituent, but is totally absent in its cultured broth and its natural host, C. kanehirae wood. On feeding with the acid-digested sawdust of C. kanehirae wood or vanillin to the broth for culture, Asaraldehyde is produced in both cultured broths. [1] Asaraldehyde shows 3.32% of prostaglandin H endoperoxide synthase-1 (COX-1) inhibitory activity and 52.69% prostaglandin H endoperoxide synthase-2 (COX-2) inhibitory activity, respectively at 100 mg/mL. Asaraldehyde shows selectivity towards COX-2 enzyme inhibition at 100 µg/mL. The COX-2/COX-1 ratio for Asaraldehyde is 17.68 at 100 µg/mL compared to solvent control. [2] Asaraldehyde down-regulates C/EBPβ, C/EBPδ, and C/EBPα. Asaraldehyde suppresses expression of PPARγ1 and PPARγ2. Asaraldehyde also significantly suppresses the expression of acetyl-CoA carboxylase (ACC). [2]

Protocol (from reference)

Cell Research:[3]
  • Cell lines: 3T3-L1 cells
  • Concentrations: 100 μg/mL
  • Incubation Time: 24 hours
  • Method: 3T3-L1 cells are seeded in 96-well plates at a concentration of 104 /well. Twenty-four hours after seeding, the cells are treated with 100 μg/mL of Asaraldehyde for 24 hours or for the whole 8-day differentiation period. Fully differentiated adipocytes are also treated with 100 μg/mL of Asaraldehyde for 24 hours-72 hours to test the cytotoxicity. At the end of treatment, cells are cultured with MTT at a final concentration of 0.5 mg/mL for another 4 hours. The purple MTT formazan is dissolved by DMSO and the absorbance at 570 nm is taken with a spectrophotometer. The absorbance is proportional to the viability of adipocytes.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 39 mg/mL
(198.77 mM)
Ethanol 16 mg/mL
(81.54 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 196.2
Formula

C10H12O4

CAS No. 4460-86-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=CC(=C(C=C1C=O)OC)OC

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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