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Asaraldehyde COX inhibitor

Asaraldehyde (Asaronaldehyde) is a natural COX-2 inhibitor, exhibiting 17-fold selectivity over COX-1.

Asaraldehyde COX inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 196.2

Purity & Quality Control

Batch: S253101 DMSO]39 mg/mL]false]Ethanol]16 mg/mL]false]Water]Insoluble]false Purity: 100%
100

Related Products

Signaling Pathway

COX Signaling Pathways

COX Signaling Pathway

Mechanism of Action

Targets
COX-2 [2]

In vitro
In vitro Asaraldehyde is found to be the most abundant constituent, but is totally absent in its cultured broth and its natural host, C. kanehirae wood. On feeding with the acid-digested sawdust of C. kanehirae wood or vanillin to the broth for culture, this compound is produced in both cultured broths. [1] It shows 3.32% of prostaglandin H endoperoxide synthase-1 (COX-1) inhibitory activity and 52.69% prostaglandin H endoperoxide synthase-2 (COX-2) inhibitory activity, respectively at 100 mg/mL. This chemical shows selectivity towards COX-2 enzyme inhibition at 100 µg/mL. The COX-2/COX-1 ratio for it is 17.68 at 100 µg/mL compared to solvent control. [2] This compound down-regulates C/EBPβ, C/EBPδ, and C/EBPα. It suppresses expression of PPARγ1 and PPARγ2. It also significantly suppresses the expression of acetyl-CoA carboxylase (ACC). [2]
Cell Research Cell lines 3T3-L1 cells
Concentrations 100 μg/mL
Incubation Time 24 hours
Method 3T3-L1 cells are seeded in 96-well plates at a concentration of 104 /well. Twenty-four hours after seeding, the cells are treated with 100 μg/mL of Asaraldehyde for 24 hours or for the whole 8-day differentiation period. Fully differentiated adipocytes are also treated with 100 μg/mL of this compound for 24 hours-72 hours to test the cytotoxicity. At the end of treatment, cells are cultured with MTT at a final concentration of 0.5 mg/mL for another 4 hours. The purple MTT formazan is dissolved by DMSO and the absorbance at 570 nm is taken with a spectrophotometer. The absorbance is proportional to the viability of adipocytes.

References
  • https://pubmed.ncbi.nlm.nih.gov/17397476/
  • https://pubmed.ncbi.nlm.nih.gov/13680840/
  • https://pubmed.ncbi.nlm.nih.gov/22746947/

Chemical Information

Molecular Weight 196.2 Formula

C10H12O4
CAS No. 4460-86-0 SDF Download SDF
Synonyms Asaronaldehyde
Smiles COC1=CC(=C(C=C1C=O)OC)OC

Storage and Stability

Storage (From the date of receipt)

In vitro
Batch:

DMSO : 39 mg/mL ( (198.77 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 16 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
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In vivo Formulation Calculator

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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