Z-IETD-FMK

Catalog No.S7314 Synonyms: Caspase-8 Inhibitor

Z-IETD-FMK Chemical Structure

Molecular Weight(MW): 654.68

Z-IETD-FMK is a specific Caspase-8 inhibitor.

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USD 197 In stock
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Cited by 8 Publications

5 Customer Reviews

  • The caspase-8 inhibitors do not rescue NB4 cells from TPEN-triggered apoptosis. NB4 cells were pre-treated with 50 μM Z-ITED-FMK or 100 μM Ac-IETD-CHO for 1 h, and then treated with 5 μM TPEN for 24 h. (A) Cell morphology was observed and recorded using an optical microscope. The scale bar represents 20 μm. A partiallty enlarged view was shown in the lower left corner of every picture. (B) Apoptosis rate was measured with Annexin V/PI dual staining by flow cytometry.

    Cell Physiol Biochem, 2017, 42(5):1822-1836. Z-IETD-FMK purchased from Selleck.

    The inhibition of caspase‑8 had no effect on the cell detachment and cell death induced by reduced-gliotoxin. The cells were treated with 5 μM reduced-gliotoxin for 24 h, in the absence and presence of the caspase‑8 inhibitor, Z-IETD-FMK, at various concentrations. Western blot analyses were performed for the indicated protein. GAPDH was used as a loading control. re-G, reduced-gliotoxin; CTNNB, β-catenin

    Int J Oncol, 2018, 52(3):1023-1032. Z-IETD-FMK purchased from Selleck.

  • sh-A20 cells were incubated with 100 ng/mL of IFN-γ for 24 h after 1 h pretreatment with z-DEVD-fmk, z-LEHD-fmk and z-IETD-fmk, respectively. Cell viability (left) was determined by MTT assay and apoptosis (right) was determined by flow cytometry. The data are represented as the mean ± SD of three independent experiments. The significance was determined by the Student’s t-test (**P<0.01).

    Drug Des Devel Ther, 2017, 11:2841-2850. Z-IETD-FMK purchased from Selleck.

    Cells were pretreated with Z-ITED-FMK (caspase-8 inhibitor, 20 μM) for 30 min before FTS(farnesylthiosalicylic acid)/DHA(Dihydroartemisinin)/ARS(artesunate) treatment or the combination treatment of DHA/ARS and FTS for 48 h and then analyzed by CCK-8 assay. *P < 0.05, **P < 0.01, and ***P < 0.001.

    PLoS One, 2017, 12(2):e0171840. Z-IETD-FMK purchased from Selleck.

  • The viability of MC3T3-E1 cells was measured by the CCK-8 assay. The cells were treated with Z-IETD-FMK (40 µM), Nec-1 (50 µM), or Z-IETD-FMK (40 µM) combined with Nec-1 (50 µM) for 1 h and then co-cultured with tumor necrosis factor-α (TNF-α) for 24 h. The control group was treated with TNF-α without any inhibitor. Data on the y-axis indicate cell viability relative to the group of cells without any treatment. *P<0.05, **P<0.01, ***P<0.001 compared with control treatment (ANOVA).

    Braz J Med Biol Res, 2018, doi:10.1590/1414-431X20187844. Z-IETD-FMK purchased from Selleck.

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Biological Activity

Description Z-IETD-FMK is a specific Caspase-8 inhibitor.
Targets
Caspase-8 [1]
In vitro

Z-IETD-FMK, which inhibits the cleavage of caspase-8 and partially inhibits the cleavage of caspase-3 and PARP, prevents the execution of apoptosis in retinal cells exposed to different apoptotic stimuli. [1] Z-IETD-FMK (50 μM) reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. [2] Inhibition of caspase-8 by Z-IETD-FMK affects the generation of activated/memory T cells and T cell cytokine production, and decreases NF-kappaB responses to TCR:CD3 engagement in T cell cultures. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 MWTGeY5kfGmxbjDhd5NigQ>? M{\zVnouUUWWRD3GUWsh[WOlZXzldoF1\XNidHjlJHBRUS2rbnT1Z4VlKGOnbHyg[IVifGhib3[gTIVxTzJiY3XscJMv NXO2UlgzOzB4N{G0OVg>
HL-60 Ml3WSpVv[3Srb36gZZN{[Xl? M3fDPVUxKM7:TR?= M2DQO|EhcA>? NVfwcm04[2:vcHzleIVtgSCkbH;jb5MhfGinIEKzMWhWSS2rbnT1Z4VlKESQQTDmdoFodWWwdHH0bY9v NXjuOlZXOzB3NUG2NlA>
MG63 MnT1R5l1d3SxeHnjbZR6KGG|c3H5 MkLlNVAh|ryP NFz5SYVx[XK2aXHscJkhemW4ZYLz[YQhVVOSLUStbY5lfWOnZDDNS|Y{KGOnbHygZ5l1d3SxeHnjbZR6 NHPuVpQzQTNyMUOwPC=>
HXO-RB44 cells NF\nVoFCeG:ydH;zbZMh[XO|YYm= NF7te5Zl\WO{ZXHz[ZMhfGinIHHwc5B1d3SrYzDyZZRmeyCrbnT1Z4VlKGK7IHL1[oFtcW5? M1XJTlI4QTB2Nkm3
Jurkat cells NUP1N4txTnWwY4Tpc44h[XO|YYm= NH\DZo02OCEQvF2= NV7SOFhlOSCq MnvHZYJwdGm|aHXzJJRp\SClbHXheoFo\SCxZjD0Roll M4rFT|I2PjN{NECx
DU 145 M4rEbmZ2dmO2aX;uJIF{e2G7 M4\xflIxKM7:TR?= NIK1c4EzPCCq MXTpcpRmenK3cITl[EB1cGViY3zlZZZi\2Vib3[gVGFTWCCrbnT1Z4VlKGK7IHXy[49{fGW{b3ygdIVzd3irZHW= M2TxT|I2PTB4Mk[1

... Click to View More Cell Line Experimental Data

In vivo In vivo, inhibition of caspase-8 by Z-IETD-FMK reduces memory/activated CD4 and CD8 T cells, and increases susceptibility to T. cruzi infection. [3] Z-IETD-FMK promotes neuronal survival and regeneration of injured retinal ganglion cells after CNS injuries. [4]

Protocol

Animal Research:

[3]

+ Expand
  • Animal Models: T. cruzi-infected mice
  • Formulation: DMSO/PBS (15%)
  • Dosages: 0.4 mg/3 days
  • Administration: --
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 91 mg/mL (138.99 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 654.68
Formula

C30H43FN4O11

CAS No. 210344-98-2
Storage powder
in solvent
Synonyms Caspase-8 Inhibitor

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID