Catalog No.S7314 Synonyms: Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK

For research use only.

Z-IETD-FMK (Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK) is a specific Caspase-8 inhibitor.

Z-IETD-FMK Chemical Structure

CAS No. 210344-98-2

Selleck's Z-IETD-FMK has been cited by 38 publications

Purity & Quality Control

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Biological Activity

Description Z-IETD-FMK (Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK) is a specific Caspase-8 inhibitor.
Caspase-8 [1]
In vitro

Z-IETD-FMK, which inhibits the cleavage of caspase-8 and partially inhibits the cleavage of caspase-3 and PARP, prevents the execution of apoptosis in retinal cells exposed to different apoptotic stimuli. [1] Z-IETD-FMK (50 μM) reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. [2] Inhibition of caspase-8 by Z-IETD-FMK affects the generation of activated/memory T cells and T cell cytokine production, and decreases NF-kappaB responses to TCR:CD3 engagement in T cell cultures. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 MnP0SpVv[3Srb36gZZN{[Xl? M1LrSnouUUWWRD3GUWsh[WOlZXzldoF1\XNidHjlJHBRUS2rbnT1Z4VlKGOnbHyg[IVifGhib3[gTIVxTzJiY3XscJMv NFjNeJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|ME[3NVQ2QCd-M{C2O|E1PTh:L3G+
HL-60 NYLzT3ZtTnWwY4Tpc44h[XO|YYm= NGfMWJQ2OCEQvF2= MnL4NUBp MUDjc41xdGW2ZXz5JIJtd2OtczD0bIUhOjNvSGXBMYlv\HWlZXSgSG5CKG[{YXft[Y51[XSrb36= NFHNOWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEW1NVYzOCd-M{C1OVE3OjB:L3G+
MG63 MWjDfZRwfG:6aXPpeJkh[XO|YYm= M4D1dlExKM7:TR?= NIXLVHRx[XK2aXHscJkhemW4ZYLz[YQhVVOSLUStbY5lfWOnZDDNS|Y{KGOnbHygZ5l1d3SxeHnjbZR6 MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTNyMUOwPEc,Ojl|MEGzNFg9N2F-
HXO-RB44 cells NFnIfYhCeG:ydH;zbZMh[XO|YYm= MkDZ[IVkemWjc3XzJJRp\SCjcH;weI91cWNicnH0[ZMhcW6mdXPl[EBjgSCkdX\hcIlv Mlz2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd7MES2PVcoRjJ5OUC0Olk4RC:jPh?=
Jurkat cells MWnGeY5kfGmxbjDhd5NigQ>? NYfHV21TPTBizszN M1i1elEhcA>? MVrhZo9tcXOqZYOgeIhmKGOuZXH2ZYdmKG:oIITCbYQ> NYrDcoYyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2N|I1ODFpPkK1OlMzPDBzPD;hQi=>
DU 145 NFXqO4NHfW6ldHnvckBie3OjeR?= NGrHXJQzOCEQvF2= Mn\qNlQhcA>? NHrON|BqdnSncoL1dJRm\CC2aHWgZ4xm[X[jZ3Wgc4YhWEGUUDDpcoR2[2WmIHL5JIVz\2:|dHXyc4wheGW{b4jp[IU> NE\SeoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUWwOlI3PSd-MkW1NFYzPjV:L3G+
In vivo In vivo, inhibition of caspase-8 by Z-IETD-FMK reduces memory/activated CD4 and CD8 T cells, and increases susceptibility to T. cruzi infection. [3] Z-IETD-FMK promotes neuronal survival and regeneration of injured retinal ganglion cells after CNS injuries. [4]

Protocol (from reference)

Animal Research:


  • Animal Models: T. cruzi-infected mice
  • Dosages: 0.4 mg/3 days
  • Administration: --

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 654.68


CAS No. 210344-98-2
Storage 3 years -20°C powder
2 years -80°C in solvent

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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