Catalog No.S7314 Synonyms: Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK

For research use only.

Z-IETD-FMK (Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK) is a specific Caspase-8 inhibitor.

Z-IETD-FMK Chemical Structure

CAS No. 210344-98-2

Selleck's Z-IETD-FMK has been cited by 34 publications

Purity & Quality Control

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Biological Activity

Description Z-IETD-FMK (Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK) is a specific Caspase-8 inhibitor.
Caspase-8 [1]
In vitro

Z-IETD-FMK, which inhibits the cleavage of caspase-8 and partially inhibits the cleavage of caspase-3 and PARP, prevents the execution of apoptosis in retinal cells exposed to different apoptotic stimuli. [1] Z-IETD-FMK (50 μM) reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. [2] Inhibition of caspase-8 by Z-IETD-FMK affects the generation of activated/memory T cells and T cell cytokine production, and decreases NF-kappaB responses to TCR:CD3 engagement in T cell cultures. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 NXjVcmVZTnWwY4Tpc44h[XO|YYm= Mn\RXk1KTVSGLV\NT{Bi[2OnbHXyZZRmeyC2aHWgVHBKNWmwZIXj[YQh[2WubDDk[YF1cCCxZjDI[ZBIOiClZXzsd{4> MlrmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB4N{G0OVgoRjNyNkexOFU5RC:jPh?=
HL-60 MlyxSpVv[3Srb36gZZN{[Xl? NIq5NHM2OCEQvF2= NWX0ZoZvOSCq NVT5OVRC[2:vcHzleIVtgSCkbH;jb5MhfGinIEKzMWhWSS2rbnT1Z4VlKESQQTDmdoFodWWwdHH0bY9v NETuR2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEW1NVYzOCd-M{C1OVE3OjB:L3G+
MG63 MoLlR5l1d3SxeHnjbZR6KGG|c3H5 NEW0XW8yOCEQvF2= NWj6NlR[eGG{dHnhcIx6KHKndnXyd4VlKE2VUD20MYlv\HWlZXSgUWc3OyClZXzsJIN6fG:2b4jpZ4l1gQ>? NFmzfFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUOwNVMxQCd-MkmzNFE{ODh:L3G+
HXO-RB44 cells MnXoRZBweHSxc3nzJIF{e2G7 MlL5[IVkemWjc3XzJJRp\SCjcH;weI91cWNicnH0[ZMhcW6mdXPl[EBjgSCkdX\hcIlv NVfhNmlJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke5NFQ3QTdpPkK3PVA1Pjl5PD;hQi=>
Jurkat cells MUnGeY5kfGmxbjDhd5NigQ>? M2D6S|UxKM7:TR?= MnriNUBp NHvGdYZi[m:uaYPo[ZMhfGinIHPs[YF3[WenIH;mJJRDcWR? NUH2SXRXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2N|I1ODFpPkK1OlMzPDBzPD;hQi=>
DU 145 NV;BXVY{TnWwY4Tpc44h[XO|YYm= NYq0SoVmOjBizszN MX:yOEBp NIDyUHZqdnSncoL1dJRm\CC2aHWgZ4xm[X[jZ3Wgc4YhWEGUUDDpcoR2[2WmIHL5JIVz\2:|dHXyc4wheGW{b4jp[IU> M{KzN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUC2NlY2Lz5{NUWwOlI3PTxxYU6=
In vivo In vivo, inhibition of caspase-8 by Z-IETD-FMK reduces memory/activated CD4 and CD8 T cells, and increases susceptibility to T. cruzi infection. [3] Z-IETD-FMK promotes neuronal survival and regeneration of injured retinal ganglion cells after CNS injuries. [4]

Protocol (from reference)

Animal Research:


  • Animal Models: T. cruzi-infected mice
  • Dosages: 0.4 mg/3 days
  • Administration: --
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 91 mg/mL
(138.99 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 654.68


CAS No. 210344-98-2
Storage 3 years -20°C powder
2 years -80°C in solvent

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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