Catalog No.S7314 Synonyms: Caspase-8 Inhibitor

Z-IETD-FMK Chemical Structure

Molecular Weight(MW): 654.68

Z-IETD-FMK is a specific Caspase-8 inhibitor.

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Cited by 8 Publications

5 Customer Reviews

  • The caspase-8 inhibitors do not rescue NB4 cells from TPEN-triggered apoptosis. NB4 cells were pre-treated with 50 μM Z-ITED-FMK or 100 μM Ac-IETD-CHO for 1 h, and then treated with 5 μM TPEN for 24 h. (A) Cell morphology was observed and recorded using an optical microscope. The scale bar represents 20 μm. A partiallty enlarged view was shown in the lower left corner of every picture. (B) Apoptosis rate was measured with Annexin V/PI dual staining by flow cytometry.

    Cell Physiol Biochem, 2017, 42(5):1822-1836. Z-IETD-FMK purchased from Selleck.

    The inhibition of caspase‑8 had no effect on the cell detachment and cell death induced by reduced-gliotoxin. The cells were treated with 5 μM reduced-gliotoxin for 24 h, in the absence and presence of the caspase‑8 inhibitor, Z-IETD-FMK, at various concentrations. Western blot analyses were performed for the indicated protein. GAPDH was used as a loading control. re-G, reduced-gliotoxin; CTNNB, β-catenin

    Int J Oncol, 2018, 52(3):1023-1032. Z-IETD-FMK purchased from Selleck.

  • sh-A20 cells were incubated with 100 ng/mL of IFN-γ for 24 h after 1 h pretreatment with z-DEVD-fmk, z-LEHD-fmk and z-IETD-fmk, respectively. Cell viability (left) was determined by MTT assay and apoptosis (right) was determined by flow cytometry. The data are represented as the mean ± SD of three independent experiments. The significance was determined by the Student’s t-test (**P<0.01).

    Drug Des Devel Ther, 2017, 11:2841-2850. Z-IETD-FMK purchased from Selleck.

    Cells were pretreated with Z-ITED-FMK (caspase-8 inhibitor, 20 μM) for 30 min before FTS(farnesylthiosalicylic acid)/DHA(Dihydroartemisinin)/ARS(artesunate) treatment or the combination treatment of DHA/ARS and FTS for 48 h and then analyzed by CCK-8 assay. *P < 0.05, **P < 0.01, and ***P < 0.001.

    PLoS One, 2017, 12(2):e0171840. Z-IETD-FMK purchased from Selleck.

  • The viability of MC3T3-E1 cells was measured by the CCK-8 assay. The cells were treated with Z-IETD-FMK (40 µM), Nec-1 (50 µM), or Z-IETD-FMK (40 µM) combined with Nec-1 (50 µM) for 1 h and then co-cultured with tumor necrosis factor-α (TNF-α) for 24 h. The control group was treated with TNF-α without any inhibitor. Data on the y-axis indicate cell viability relative to the group of cells without any treatment. *P<0.05, **P<0.01, ***P<0.001 compared with control treatment (ANOVA).

    Braz J Med Biol Res, 2018, doi:10.1590/1414-431X20187844. Z-IETD-FMK purchased from Selleck.

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Biological Activity

Description Z-IETD-FMK is a specific Caspase-8 inhibitor.
Caspase-8 [1]
In vitro

Z-IETD-FMK, which inhibits the cleavage of caspase-8 and partially inhibits the cleavage of caspase-3 and PARP, prevents the execution of apoptosis in retinal cells exposed to different apoptotic stimuli. [1] Z-IETD-FMK (50 μM) reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. [2] Inhibition of caspase-8 by Z-IETD-FMK affects the generation of activated/memory T cells and T cell cytokine production, and decreases NF-kappaB responses to TCR:CD3 engagement in T cell cultures. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 MWTGeY5kfGmxbjDhd5NigQ>? M{\zVnouUUWWRD3GUWsh[WOlZXzldoF1\XNidHjlJHBRUS2rbnT1Z4VlKGOnbHyg[IVifGhib3[gTIVxTzJiY3XscJMv NXO2UlgzOzB4N{G0OVg>
HL-60 Ml3WSpVv[3Srb36gZZN{[Xl? M3fDPVUxKM7:TR?= M2DQO|EhcA>? NVfwcm04[2:vcHzleIVtgSCkbH;jb5MhfGinIEKzMWhWSS2rbnT1Z4VlKESQQTDmdoFodWWwdHH0bY9v NXjuOlZXOzB3NUG2NlA>
MG63 MnT1R5l1d3SxeHnjbZR6KGG|c3H5 MkLlNVAh|ryP NFz5SYVx[XK2aXHscJkhemW4ZYLz[YQhVVOSLUStbY5lfWOnZDDNS|Y{KGOnbHygZ5l1d3SxeHnjbZR6 NHPuVpQzQTNyMUOwPC=>
HXO-RB44 cells NF\nVoFCeG:ydH;zbZMh[XO|YYm= NF7te5Zl\WO{ZXHz[ZMhfGinIHHwc5B1d3SrYzDyZZRmeyCrbnT1Z4VlKGK7IHL1[oFtcW5? M1XJTlI4QTB2Nkm3
Jurkat cells NUP1N4txTnWwY4Tpc44h[XO|YYm= NH\DZo02OCEQvF2= NV7SOFhlOSCq MnvHZYJwdGm|aHXzJJRp\SClbHXheoFo\SCxZjD0Roll M4rFT|I2PjN{NECx
DU 145 M4rEbmZ2dmO2aX;uJIF{e2G7 M4\xflIxKM7:TR?= NIK1c4EzPCCq MXTpcpRmenK3cITl[EB1cGViY3zlZZZi\2Vib3[gVGFTWCCrbnT1Z4VlKGK7IHXy[49{fGW{b3ygdIVzd3irZHW= M2TxT|I2PTB4Mk[1

... Click to View More Cell Line Experimental Data

In vivo In vivo, inhibition of caspase-8 by Z-IETD-FMK reduces memory/activated CD4 and CD8 T cells, and increases susceptibility to T. cruzi infection. [3] Z-IETD-FMK promotes neuronal survival and regeneration of injured retinal ganglion cells after CNS injuries. [4]


Animal Research:


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  • Animal Models: T. cruzi-infected mice
  • Formulation: DMSO/PBS (15%)
  • Dosages: 0.4 mg/3 days
  • Administration: --
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 91 mg/mL (138.99 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 654.68


CAS No. 210344-98-2
Storage powder
in solvent
Synonyms Caspase-8 Inhibitor

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID