Z-IETD-FMK

Catalog No.S7314 Synonyms: Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK

For research use only.

Z-IETD-FMK (Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK) is a specific Caspase-8 inhibitor.

Z-IETD-FMK Chemical Structure

CAS No. 210344-98-2

Selleck's Z-IETD-FMK has been cited by 34 publications

Purity & Quality Control

Choose Selective Caspase Inhibitors

Other Caspase Products

Biological Activity

Description Z-IETD-FMK (Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK) is a specific Caspase-8 inhibitor.
Targets
Caspase-8 [1]
In vitro

Z-IETD-FMK, which inhibits the cleavage of caspase-8 and partially inhibits the cleavage of caspase-3 and PARP, prevents the execution of apoptosis in retinal cells exposed to different apoptotic stimuli. [1] Z-IETD-FMK (50 μM) reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. [2] Inhibition of caspase-8 by Z-IETD-FMK affects the generation of activated/memory T cells and T cell cytokine production, and decreases NF-kappaB responses to TCR:CD3 engagement in T cell cultures. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 NXjVcmVZTnWwY4Tpc44h[XO|YYm= Mn\RXk1KTVSGLV\NT{Bi[2OnbHXyZZRmeyC2aHWgVHBKNWmwZIXj[YQh[2WubDDk[YF1cCCxZjDI[ZBIOiClZXzsd{4> MlrmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB4N{G0OVgoRjNyNkexOFU5RC:jPh?=
HL-60 MlyxSpVv[3Srb36gZZN{[Xl? NIq5NHM2OCEQvF2= NWX0ZoZvOSCq NVT5OVRC[2:vcHzleIVtgSCkbH;jb5MhfGinIEKzMWhWSS2rbnT1Z4VlKESQQTDmdoFodWWwdHH0bY9v NETuR2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEW1NVYzOCd-M{C1OVE3OjB:L3G+
MG63 MoLlR5l1d3SxeHnjbZR6KGG|c3H5 NEW0XW8yOCEQvF2= NWj6NlR[eGG{dHnhcIx6KHKndnXyd4VlKE2VUD20MYlv\HWlZXSgUWc3OyClZXzsJIN6fG:2b4jpZ4l1gQ>? NFmzfFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUOwNVMxQCd-MkmzNFE{ODh:L3G+
HXO-RB44 cells MnXoRZBweHSxc3nzJIF{e2G7 MlL5[IVkemWjc3XzJJRp\SCjcH;weI91cWNicnH0[ZMhcW6mdXPl[EBjgSCkdX\hcIlv NVfhNmlJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke5NFQ3QTdpPkK3PVA1Pjl5PD;hQi=>
Jurkat cells MUnGeY5kfGmxbjDhd5NigQ>? M2D6S|UxKM7:TR?= MnriNUBp NHvGdYZi[m:uaYPo[ZMhfGinIHPs[YF3[WenIH;mJJRDcWR? NUH2SXRXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2N|I1ODFpPkK1OlMzPDBzPD;hQi=>
DU 145 NV;BXVY{TnWwY4Tpc44h[XO|YYm= NYq0SoVmOjBizszN MX:yOEBp NIDyUHZqdnSncoL1dJRm\CC2aHWgZ4xm[X[jZ3Wgc4YhWEGUUDDpcoR2[2WmIHL5JIVz\2:|dHXyc4wheGW{b4jp[IU> M{KzN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUC2NlY2Lz5{NUWwOlI3PTxxYU6=
In vivo In vivo, inhibition of caspase-8 by Z-IETD-FMK reduces memory/activated CD4 and CD8 T cells, and increases susceptibility to T. cruzi infection. [3] Z-IETD-FMK promotes neuronal survival and regeneration of injured retinal ganglion cells after CNS injuries. [4]

Protocol (from reference)

Animal Research:

[3]

  • Animal Models: T. cruzi-infected mice
  • Dosages: 0.4 mg/3 days
  • Administration: --
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 91 mg/mL
(138.99 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 654.68
Formula

C30H43FN4O11

CAS No. 210344-98-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC(C)C(C(=O)NC(CCC(=O)OC)C(=O)NC(C(C)O)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC1=CC=CC=C1

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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