Catalog No.S7314 Synonyms: Caspase-8 Inhibitor
Molecular Weight(MW): 654.68
Z-IETD-FMK is a specific Caspase-8 inhibitor.
Cited by 8 Publications
5 Customer Reviews
The caspase-8 inhibitors do not rescue NB4 cells from TPEN-triggered apoptosis. NB4 cells were pre-treated with 50 μM Z-ITED-FMK or 100 μM Ac-IETD-CHO for 1 h, and then treated with 5 μM TPEN for 24 h. (A) Cell morphology was observed and recorded using an optical microscope. The scale bar represents 20 μm. A partiallty enlarged view was shown in the lower left corner of every picture. (B) Apoptosis rate was measured with Annexin V/PI dual staining by flow cytometry.
Cell Physiol Biochem, 2017, 42(5):1822-1836. Z-IETD-FMK purchased from Selleck.
The inhibition of caspase‑8 had no effect on the cell detachment and cell death induced by reduced-gliotoxin. The cells were treated with 5 μM reduced-gliotoxin for 24 h, in the absence and presence of the caspase‑8 inhibitor, Z-IETD-FMK, at various concentrations. Western blot analyses were performed for the indicated protein. GAPDH was used as a loading control. re-G, reduced-gliotoxin; CTNNB, β-catenin
Int J Oncol, 2018, 52(3):1023-1032. Z-IETD-FMK purchased from Selleck.
sh-A20 cells were incubated with 100 ng/mL of IFN-γ for 24 h after 1 h pretreatment with z-DEVD-fmk, z-LEHD-fmk and z-IETD-fmk, respectively. Cell viability (left) was determined by MTT assay and apoptosis (right) was determined by flow cytometry. The data are represented as the mean ± SD of three independent experiments. The significance was determined by the Student’s t-test (**P<0.01).
Drug Des Devel Ther, 2017, 11:2841-2850. Z-IETD-FMK purchased from Selleck.
Cells were pretreated with Z-ITED-FMK (caspase-8 inhibitor, 20 μM) for 30 min before FTS(farnesylthiosalicylic acid)/DHA(Dihydroartemisinin)/ARS(artesunate) treatment or the combination treatment of DHA/ARS and FTS for 48 h and then analyzed by CCK-8 assay. *P < 0.05, **P < 0.01, and ***P < 0.001.
PLoS One, 2017, 12(2):e0171840. Z-IETD-FMK purchased from Selleck.
The viability of MC3T3-E1 cells was measured by the CCK-8 assay. The cells were treated with Z-IETD-FMK (40 µM), Nec-1 (50 µM), or Z-IETD-FMK (40 µM) combined with Nec-1 (50 µM) for 1 h and then co-cultured with tumor necrosis factor-α (TNF-α) for 24 h. The control group was treated with TNF-α without any inhibitor. Data on the y-axis indicate cell viability relative to the group of cells without any treatment. *P<0.05, **P<0.01, ***P<0.001 compared with control treatment (ANOVA).
Braz J Med Biol Res, 2018, doi:10.1590/1414-431X20187844. Z-IETD-FMK purchased from Selleck.
Purity & Quality Control
Choose Selective Caspase Inhibitors
|Description||Z-IETD-FMK is a specific Caspase-8 inhibitor.|
Z-IETD-FMK, which inhibits the cleavage of caspase-8 and partially inhibits the cleavage of caspase-3 and PARP, prevents the execution of apoptosis in retinal cells exposed to different apoptotic stimuli.  Z-IETD-FMK (50 μM) reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3.  Inhibition of caspase-8 by Z-IETD-FMK affects the generation of activated/memory T cells and T cell cytokine production, and decreases NF-kappaB responses to TCR:CD3 engagement in T cell cultures. 
|In vivo||In vivo, inhibition of caspase-8 by Z-IETD-FMK reduces memory/activated CD4 and CD8 T cells, and increases susceptibility to T. cruzi infection.  Z-IETD-FMK promotes neuronal survival and regeneration of injured retinal ganglion cells after CNS injuries. |
|In vitro||DMSO||91 mg/mL (138.99 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).
Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2
Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.