Catalog No.S7314 Synonyms: Caspase-8 Inhibitor
Molecular Weight(MW): 654.68
Z-IETD-FMK is a specific Caspase-8 inhibitor.
Cited by 16 Publications
5 Customer Reviews
The caspase-8 inhibitors do not rescue NB4 cells from TPEN-triggered apoptosis. NB4 cells were pre-treated with 50 μM Z-ITED-FMK or 100 μM Ac-IETD-CHO for 1 h, and then treated with 5 μM TPEN for 24 h. (A) Cell morphology was observed and recorded using an optical microscope. The scale bar represents 20 μm. A partiallty enlarged view was shown in the lower left corner of every picture. (B) Apoptosis rate was measured with Annexin V/PI dual staining by flow cytometry.
Cell Physiol Biochem, 2017, 42(5):1822-1836. Z-IETD-FMK purchased from Selleck.
The inhibition of caspase‑8 had no effect on the cell detachment and cell death induced by reduced-gliotoxin. The cells were treated with 5 μM reduced-gliotoxin for 24 h, in the absence and presence of the caspase‑8 inhibitor, Z-IETD-FMK, at various concentrations. Western blot analyses were performed for the indicated protein. GAPDH was used as a loading control. re-G, reduced-gliotoxin; CTNNB, β-catenin
Int J Oncol, 2018, 52(3):1023-1032. Z-IETD-FMK purchased from Selleck.
sh-A20 cells were incubated with 100 ng/mL of IFN-γ for 24 h after 1 h pretreatment with z-DEVD-fmk, z-LEHD-fmk and z-IETD-fmk, respectively. Cell viability (left) was determined by MTT assay and apoptosis (right) was determined by flow cytometry. The data are represented as the mean ± SD of three independent experiments. The significance was determined by the Student’s t-test (**P<0.01).
Drug Des Devel Ther, 2017, 11:2841-2850. Z-IETD-FMK purchased from Selleck.
Cells were pretreated with Z-ITED-FMK (caspase-8 inhibitor, 20 μM) for 30 min before FTS(farnesylthiosalicylic acid)/DHA(Dihydroartemisinin)/ARS(artesunate) treatment or the combination treatment of DHA/ARS and FTS for 48 h and then analyzed by CCK-8 assay. *P < 0.05, **P < 0.01, and ***P < 0.001.
PLoS One, 2017, 12(2):e0171840. Z-IETD-FMK purchased from Selleck.
The viability of MC3T3-E1 cells was measured by the CCK-8 assay. The cells were treated with Z-IETD-FMK (40 µM), Nec-1 (50 µM), or Z-IETD-FMK (40 µM) combined with Nec-1 (50 µM) for 1 h and then co-cultured with tumor necrosis factor-α (TNF-α) for 24 h. The control group was treated with TNF-α without any inhibitor. Data on the y-axis indicate cell viability relative to the group of cells without any treatment. *P<0.05, **P<0.01, ***P<0.001 compared with control treatment (ANOVA).
Braz J Med Biol Res, 2018, doi:10.1590/1414-431X20187844. Z-IETD-FMK purchased from Selleck.
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Choose Selective Caspase Inhibitors
|Description||Z-IETD-FMK is a specific Caspase-8 inhibitor.|
Z-IETD-FMK, which inhibits the cleavage of caspase-8 and partially inhibits the cleavage of caspase-3 and PARP, prevents the execution of apoptosis in retinal cells exposed to different apoptotic stimuli.  Z-IETD-FMK (50 μM) reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3.  Inhibition of caspase-8 by Z-IETD-FMK affects the generation of activated/memory T cells and T cell cytokine production, and decreases NF-kappaB responses to TCR:CD3 engagement in T cell cultures. 
|In vivo||In vivo, inhibition of caspase-8 by Z-IETD-FMK reduces memory/activated CD4 and CD8 T cells, and increases susceptibility to T. cruzi infection.  Z-IETD-FMK promotes neuronal survival and regeneration of injured retinal ganglion cells after CNS injuries. |
|In vitro||DMSO||91 mg/mL (138.99 mM)|
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