Z-DEVD-FMK

Catalog No.S7312 Synonyms: Caspase-3 Inhibitor

Z-DEVD-FMK Chemical Structure

Molecular Weight(MW): 668.66

Z-DEVD-FMK is a specific, irreversible Caspase-3 inhibitor, and also shows potent inhibition on caspase-6, caspase-7, caspase-8, and caspase-10.

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Cited by 25 Publications

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Biological Activity

Description Z-DEVD-FMK is a specific, irreversible Caspase-3 inhibitor, and also shows potent inhibition on caspase-6, caspase-7, caspase-8, and caspase-10.
Targets
Caspase-3 [1]
In vitro

Z-DEVD-FMK (1–200 μM) inhibits D4-GDI cleavage and apoptosis in a concentration-dependent manner. [1] Z-DEVD-FMK reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. [3] Z-DEVD-FMK (100μM) attenuates OxyHb-induced cell detachment, reduced caspase-2 and -3 activities, abolishes OxyHb-induced DNA ladders, and prevents OxyHb-induced cleavage of PARP in cultured brain microvessel endothelial cells. [4] Z-DEVD-FMK (100 μM) blocks MPP+-induced increases in caspase-3 enzyme activity. Z-DEVD-FMK dose dependently blocks 6-OHDA-induced apoptotic cell death with IC50 of 18 μM. [5]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
duck embryo fibroblasts (DEFs) Ml;jSpVv[3Srb36gZZN{[Xl? M1XXRlIhcMLi M1jKPYVn\mWldHn2[Yx6KHC{b33veIVlKH[rcnHsJJJmeGyrY3H0bY9v M3XETlMxQDF|NUCw
OSCC cell MWrBdI9xfG:|aYOgZZN{[Xl? NGLDTnczOCEEtV2= M2jNZlQ5KGh? MlradoVlfWOnIITo[UBi[3Srdnn0fUBw\iClYYPwZZNmKDNiYX7kJINie3Cjc3WgPS=> NX7WNo9[OzB3N{O5O|g>
A549 MlTYSpVv[3Srb36gZZN{[Xl? MYSyOUDPxE1? M3Kx[lI1KGh? MnG5d4lodmmoaXPhcpRtgSCrbnjpZol1KFKRUzDn[Y5memG2aX;uJIlvKEF3NEmgZ4VtdHN? NVHYUlN5OzB{NEC3NFk>
HCT116 cells MmLUSpVv[3Srb36gZZN{[Xl? MXm1NQKBkc7:TR?= NHP2bFAzKGh? MkHlZ491emWjdH3lcpQhf2m2aDDYfYxweGmwZTD0c{BxemW4ZX70[UB1cGVieInsc5BqdmVvaX7keYNm\CCrbnPy[YF{cW6pIHHwc5B1d3Orcx?= Mn3CNlk{PjJ4Nke=
K562 cells MXfGeY5kfGmxbjDhd5NigQ>? MnnmNkBp NVjnPWVZcW6lcnXhd4VlKFJwII\ldo5q[2mobIXhJIV5fHKjY4StbY5lfWOnZDDj[YxtKHC{b3zp[oVz[XSrb36= Ml;CNlk{Ojh|OEe=
THP-1 cells M2S4U2Z2dmO2aX;uJIF{e2G7 NELhbW82OCEQvF2= NHjBT24zKGh? MVPzeYIuTzFicHXhb{BKdmirYnn0bY9vKGmwIFTNVWEhcW6mdXPl[EBk\WyuIHTlZZRp NVLJZXo2Ojl{MkWxN|Y>

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot
HAUSP / Kinesin 5B / GEP100 / SDCCAG3 / PARD3 / Lamin A/C / PARP ; 

PubMed: 24195789     


HeLa cells were treated with 1 μM STS for 24 hr in the presence or absence of the 100 μM caspase inhibitors (Z-VEID-FMK; caspase-6 inhibitor, Z-DEVD-FMK; caspase-3/-7 inhibitor, Z-VAD-FMK; pan-caspase inhibitor) pretreated to cells for 3 hr. After 24 hr, 䲧疝Ỵ疞㧀疜膉痘 瘿⟸෕ᾰƌ෕Ð 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෕Ð鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ뾠Ղ䐺痖暼瘿뾠Ղᾰƌ 뾠Ղà㺣痖뾠Ղ€𢡄뾤Ղ€䀷痗뾤Ղ౴뾤Ղ㵶痗뾤Ղ뺖᎒泌Itemｾ᎒

CLK3 / ADH4 / MDH1; 

PubMed: 24195789     


HeLa cells were treated with 1 μM STS for 24 hr in the presence or absence of the 100 μM caspase inhibitors (Z-VEID-FMK; caspase-6 inhibitor, Z-DEVD-FMK; caspase-3/-7 inhibitor, Z-VAD-FMK; pan-caspase inhibitor) pretreated to cells for 3 hr. After 24 hr, 䲧疝Ỵ疞㧀疜膉痘 瘿뙠ෆᾰƌෆĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ෆĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ⟸෕䐺痖暼

ORF57 / caspase 7 / caspase 3; 

PubMed: 20159985     


Inhibition of ORF57 cleavage by specific caspase inhibitors. BCBL-1 cells treated with VA in the presence of 50 μmpeptide-based caspase inhibitors, including pan-caspase inhibitor z-VAD-fmk, caspase-3/7 inhibitor z-DEVD-fmk , caspase-3/6 inhibitor z-VEID-䲧疝Ỵ疞㧀疜膉痘 瘿뙠ෆᾰƌෆĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ෆĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ

24195789 20159985
In vivo Z-DEVD-FMK, before and after injury, markedly reduces post-traumatic apoptosis, and significantly improved neurological recovery. [2]

Protocol

Kinase Assay:[5]
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Caspase activity assay :

Caspase-3 and caspase-9 activities are measured using fluorescent-based substrate. After treatment, the cells are resuspended in lysis buffer (50 mM Tris HCl, 1 mM EDTA, and 10 mM EGTA) containing 10 mM digitonin for 20 min at 37°C. Supernatants are treated with either of the fluorogenic substrates Ac-DEVD-AFC for caspase-3 or Ac-LEHD-AFC for caspase-9 for 1 h at 37°C and fluorescence is measured at excitation at 400 nm and emission at 505 nm using a Gemini XS fluorescence plate reade
Cell Research:[5]
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  • Cell lines: N27 cells
  • Concentrations: ~50 μM
  • Incubation Time: 24 hours
  • Method: N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT (3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl tetrazolium bromide) assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/ml MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader.
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: Male Sprague Dawley rats with Brain trauma.
  • Formulation: DMSO
  • Dosages: 160 ng
  • Administration: Intracerebroventricular administration
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (149.55 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 400+5% Tween 80+ddH2O
For best results, use promptly after mixing. 		1mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 668.66
Formula

C30H41FN4O12
CAS No. 210344-95-9
Storage powder
in solvent
Synonyms Caspase-3 Inhibitor

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID