Z-DEVD-FMK

Catalog No.S7312 Synonyms: Caspase-3 Inhibitor

For research use only.

Z-DEVD-FMK (Caspase-3 Inhibitor) is a specific, irreversible Caspase-3 inhibitor, and also shows potent inhibition on caspase-6, caspase-7, caspase-8, and caspase-10.

Z-DEVD-FMK Chemical Structure

CAS No. 210344-95-9

Selleck's Z-DEVD-FMK has been cited by 60 publications

Purity & Quality Control

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Biological Activity

Description Z-DEVD-FMK (Caspase-3 Inhibitor) is a specific, irreversible Caspase-3 inhibitor, and also shows potent inhibition on caspase-6, caspase-7, caspase-8, and caspase-10.
Targets
Caspase-3 [1]
In vitro

Z-DEVD-FMK (1–200 μM) inhibits D4-GDI cleavage and apoptosis in a concentration-dependent manner. [1] Z-DEVD-FMK reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. [3] Z-DEVD-FMK (100μM) attenuates OxyHb-induced cell detachment, reduced caspase-2 and -3 activities, abolishes OxyHb-induced DNA ladders, and prevents OxyHb-induced cleavage of PARP in cultured brain microvessel endothelial cells. [4] Z-DEVD-FMK (100 μM) blocks MPP+-induced increases in caspase-3 enzyme activity. Z-DEVD-FMK dose dependently blocks 6-OHDA-induced apoptotic cell death with IC50 of 18 μM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
duck embryo fibroblasts (DEFs) M{nVdGZ2dmO2aX;uJIF{e2G7 NIm2R5czKGkEoB?= NGPHb4Nm\m[nY4TpeoVtgSCycn;tc5Rm\CC4aYLhcEBz\XCuaXPheIlwdg>? MnPBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB6MUO1NFAoRjNyOEGzOVAxRC:jPh?=
OSCC cell NGnJZnNCeG:ydH;zbZMh[XO|YYm= MlPXNlAhyrWP NX;U[ZJTPDhiaB?= MYPy[YR2[2VidHjlJIFkfGm4aYT5JI9nKGOjc4Dhd4UhOyCjbnSgZ4F{eGG|ZTC5 NX2weVNlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C1O|M6PzhpPkOwOVc{QTd6PD;hQi=>
A549 MYjGeY5kfGmxbjDhd5NigQ>? NGH4XlgzPSEQvF2= NHHOeoMzPCCq NXXUPVBXe2mpbnnmbYNidnSueTDpcohq[mm2IGLPV{Bo\W6ncnH0bY9vKGmwIFG1OFkh[2WubIO= MmjlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{NEC3NFkoRjNyMkSwO|A6RC:jPh?=
HCT116 cells M4XiR2Z2dmO2aX;uJIF{e2G7 NHXIbHc2OOLCid88US=> NVnyPHNFOiCq NU\6THRN[2:2cnXheI1mdnRid3n0bEBZgWyxcHnu[UB1dyCycnX2[Y51\SC2aHWgfJltd3CrbnWtbY5lfWOnZDDpcoNz\WG|aX7nJIFxd3C2b4Ppdy=> NUnTcZJERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmzOlI3PjdpPkK5N|YzPjZ5PD;hQi=>
K562 cells NHfF[2tHfW6ldHnvckBie3OjeR?= NUmzdY9POiCq M32wfolv[3KnYYPl[EBTNiC4ZYLubYNq\my3YTDlfJRz[WO2LXnu[JVk\WRiY3XscEBxem:uaX\ldoF1cW:w MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTN{OEO4O{c,Ojl|MkizPFc9N2F-
THP-1 cells MYTGeY5kfGmxbjDhd5NigQ>? NVPWZpBQPTBizszN M{PvWVIhcA>? MoPNd5VjNUdzIIDlZYshUW6qaXLpeIlwdiCrbjDEUXFCKGmwZIXj[YQh[2WubDDk[YF1cA>? M33UUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MkK1NVM3Lz5{OUKyOVE{PjxxYU6=
Assay
Methods Test Index PMID
Western blot HAUSP / Kinesin 5B / GEP100 / SDCCAG3 / PARD3 / Lamin A/C / PARP ; CLK3 / ADH4 / MDH1 ; ORF57 / caspase 7 / caspase 3 24195789 20159985
In vivo Z-DEVD-FMK, before and after injury, markedly reduces post-traumatic apoptosis, and significantly improved neurological recovery. [2]

Protocol (from reference)

Kinase Assay:[5]
  • Caspase activity assay :

    Caspase-3 and caspase-9 activities are measured using fluorescent-based substrate. After treatment, the cells are resuspended in lysis buffer (50 mM Tris HCl, 1 mM EDTA, and 10 mM EGTA) containing 10 mM digitonin for 20 min at 37°C. Supernatants are treated with either of the fluorogenic substrates Ac-DEVD-AFC for caspase-3 or Ac-LEHD-AFC for caspase-9 for 1 h at 37°C and fluorescence is measured at excitation at 400 nm and emission at 505 nm using a Gemini XS fluorescence plate reade

Cell Research:[5]
  • Cell lines: N27 cells
  • Concentrations: ~50 μM
  • Incubation Time: 24 hours
  • Method: N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT (3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl tetrazolium bromide) assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/ml MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader.
Animal Research:[2]
  • Animal Models: Male Sprague Dawley rats with Brain trauma.
  • Dosages: 160 ng
  • Administration: Intracerebroventricular administration

Solubility (25°C)

In vitro

DMSO 100 mg/mL
(149.55 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 400+5% Tween 80+ddH2O
For best results, use promptly after mixing.

1mg/mL

Chemical Information

Molecular Weight 668.66
Formula

C30H41FN4O12

CAS No. 210344-95-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)C(C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CCC(=O)OC)NC(=O)C(CC(=O)OC)NC(=O)OCC1=CC=CC=C1

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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