Q-VD-Oph

Catalog No.S7311 Synonyms: Quinoline-Val-Asp-Difluorophenoxymethylketone

For research use only.

Q-VD-Oph (Quinoline-Val-Asp-Difluorophenoxymethylketone) is a potent pan-caspase inhibitor with IC50 ranged from 25 to 400 nM for caspases 1,3,8, and 9. Q-VD-OPh can inhibits HIV infection.

Q-VD-Oph Chemical Structure

CAS No. 1135695-98-5

Selleck's Q-VD-Oph has been cited by 47 publications

Purity & Quality Control

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Biological Activity

Description Q-VD-Oph (Quinoline-Val-Asp-Difluorophenoxymethylketone) is a potent pan-caspase inhibitor with IC50 ranged from 25 to 400 nM for caspases 1,3,8, and 9. Q-VD-OPh can inhibits HIV infection.
Targets
Caspase-1 [1] Caspase-3 [1] Caspase-8 [1] Caspase-9 [1]
25 nM-400 nM 25 nM-400 nM 25 nM-400 nM 25 nM-400 nM
In vitro

Q-VD-OPh (5-100 μM) potently inhibits Actinomycin D-induced DNA laddering and subsequent apoptosis with minimal toxicity in WEHI 231 cells. Q-VD-OPh prevents caspase mediated cleavage of PARP and activation of the major initiator and effector caspases. [2] Q-VD-OPh protects cardiac myocytes from virus-induced apoptosis in vitro. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
JEG3 cells NIHE[GZHfW6ldHnvckBie3OjeR?= NF76[oY2OOLCid88US=> NV3DSVMx[WK{b3fheIlwdiCxZjDjZZNx[XOnLUigZ4xm[X[jZ3Wg[pJwdSCrdIOgOVXjiImtRHGgdJJw\m:{bTD0c{BqfHNiMUlihKlsTGFiYXP0bZZmKG[{YXft[Y51 M2Pu[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUWxOFY4Lz5{OEG1NVQ3PzxxYU6=
MV4-11 cells NGXVb2dE\WyuII\pZYJqdGm2eTDhd5NigQ>? NYLJNlRWOjVizszN NUTvbXRzOSCqb4Xy M2LnWpBzd3SnY4Tpc44h[2WubIOg[pJwdSClYYPwZZNmNWSncHXu[IVvfCClZXzsJIRm[XSq M1rydVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5OUW2N|g4Lz5{N{m1OlM5PzxxYU6=
SH-SY5Y M{GwOmN6fG:2b4jpZ4l1gSCjc4PhfS=> MoToOUDPxE1? NUjCPJE{PDhiaB?= MW\RMXZFNU:SaDDz[Y5{cXSrenXzJHNJNVO\NWmgZ4VtdHNidH:gcoVkem:|aYOgZ4F2e2WmIHL5JIN6eGW{bXX0bJJqdg>? NUPPPHFpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNFc2OjZpPkK3NFA4PTJ4PD;hQi=>
MEFs NH7STmJHfW6ldHnvckBie3OjeR?= NXrOXIhkOjBizszNxsA> MX;lfIhq[mm2ZXSgZ4hiemGldHXybZN1cWNic4Ty[ZN{NWmwZIXj[YQh\2WwZYLheIlwdiCjbnSgdpVxfHW{ZTDv[kBvfWOuZXHyJIJ2[mKuZYO= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR6MkC2PEc,OjV2OEKwOlg9N2F-
Assay
Methods Test Index PMID
Western blot pS-Akt / Akt / pS6 / LC3B 24682219
Immunofluorescence LC3B 24682219
In vivo Q-VD-OPh inhibits caspase-1 activity, IL-18 protein expression, and neutrophil infiltration during ischemic ARF in mice. [3] In vivo, caspase inhibition by Q-VD-OPh provides protection from virus-induced myocardial injury, with a significant reduction in caspase-3 activity. [4] In TgCRND8 mice, Q-VD-OPh inhibits caspase-7 activation and limits the pathological changes associated with tau, including caspase cleavage. [5]

Protocol (from reference)

Cell Research:[2]
  • Cell lines: WEHI-231 cells
  • Concentrations: ~1000 μM
  • Incubation Time: 4 hours
  • Method: Caspase inhibitors are added at the indicated concentrations 30 minutes prior to the addition of apoptotic stimuli. Viability and cell number are determined by trypan blue exclusion from three random fields of greater than 200 cells/field. All experiments are performed a minimum of three times.
Animal Research:[3]
  • Animal Models: C57BL/6 mice
  • Dosages: ~120 mg/kg
  • Administration: Intraperitoneal administration

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 513.49
Formula
C26H25F2N3O6
CAS No. 1135695-98-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)C(C(=O)NC(CC(=O)O)C(=O)COC1=C(C=CC=C1F)F)NC(=O)C2=NC3=CC=CC=C3C=C2

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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