Z-YVAD-FMK

Catalog No.S8507

For research use only.

Z-YVAD-FMK is a cell-permeable and irreversible caspase-1 specific inhibitor with anti-inflammatory and anti-tumor activities.

Z-YVAD-FMK Chemical Structure

CAS No. 210344-97-1

Selleck's Z-YVAD-FMK has been cited by 3 Publications

Purity & Quality Control

Choose Selective Caspase Inhibitors

Other Caspase Products

Biological Activity

Description Z-YVAD-FMK is a cell-permeable and irreversible caspase-1 specific inhibitor with anti-inflammatory and anti-tumor activities.
Targets
caspase-1 [1]
()
In vitro

Z-YVAD-FMK treatment significantly reduces the increased caspase-1 activity induced by LPS+Aβ. Z-YVAD-FMK remarkably suppresses the protein expressions of cleaved IL-1β, cleaved IL-18, and Iba-1.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: BV2 cells
  • Concentrations: 5 μM
  • Incubation Time: 1 h
  • Method:

    BV2 cells are treated without or with EGCG (10 μM) or/and Z-YVAD-FMK (5 μM) for 1 h, followed by stimulationwith LPS (1 μg/mL) for 1 h and Aβ1-42 (10 μM) for 6 h. The cells are harvested at room temperature, and the protein concentration is determined by the Bradfordmethod. A calibration curve is constructed, and caspase-1 activity is calculated according to it.

  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 100 mg/mL
(158.56 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 630.66
Formula

C31H39FN4O9

CAS No. 210344-97-1
Storage 3 years -20°C powder
2 years -80°C in solvent

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Z-YVAD-FMK | Z-YVAD-FMK supplier | purchase Z-YVAD-FMK | Z-YVAD-FMK cost | Z-YVAD-FMK manufacturer | order Z-YVAD-FMK | Z-YVAD-FMK distributor