Z-YVAD-FMK

For research use only.

Catalog No.S8507

3 publications

Z-YVAD-FMK Chemical Structure

CAS No. 210344-97-1

Z-YVAD-FMK is a cell-permeable and irreversible caspase-1 specific inhibitor with anti-inflammatory and anti-tumor activities.

Selleck's Z-YVAD-FMK has been cited by 3 publications

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Biological Activity

Description Z-YVAD-FMK is a cell-permeable and irreversible caspase-1 specific inhibitor with anti-inflammatory and anti-tumor activities.
Targets
caspase-1 [1]
()
In vitro

Z-YVAD-FMK treatment significantly reduces the increased caspase-1 activity induced by LPS+Aβ. Z-YVAD-FMK remarkably suppresses the protein expressions of cleaved IL-1β, cleaved IL-18, and Iba-1.[1]

Protocol

Cell Research:

[1]

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  • Cell lines: BV2 cells
  • Concentrations: 5 μM
  • Incubation Time: 1 h
  • Method:

    BV2 cells are treated without or with EGCG (10 μM) or/and Z-YVAD-FMK (5 μM) for 1 h, followed by stimulationwith LPS (1 μg/mL) for 1 h and Aβ1-42 (10 μM) for 6 h. The cells are harvested at room temperature, and the protein concentration is determined by the Bradfordmethod. A calibration curve is constructed, and caspase-1 activity is calculated according to it.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (158.56 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 630.66
Formula

C31H39FN4O9

CAS No. 210344-97-1
Storage powder
in solvent
Synonyms N/A

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID