Z-VAD-FMK

Catalog No.S7023

Z-VAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.

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Z-VAD-FMK Chemical Structure

Z-VAD-FMK Chemical Structure
Molecular Weight: 467.49

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Product Information

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Product Description

Biological Activity

Description Z-VAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.
Targets Pan-caspase [1]
(THP.1, Jurkat T-cells)
In vitro Z-VAD-FMK (10 mM) inhibits apoptosis in THP.1 cells. Z-VAD-FMK (10 μM) inhibits activation of PARP protease activity in control THP.1 cell lysates. Z-VAD-FMK (10 mM) inhibits the processing of CPP32 in intact THP.1 and Jurkat cells. [1] Z-VAD-FMK (50 μM) cotreatment abolishes the apoptotic morphology of camptothecin-treated HL60 cells. Z-VAD-FMK (50 μM) blocks camptothecin-induced DNA fragmentation in HL60 cells. [2] Z-VAD-FMK (50 μM) inhibits cell death following dSMN dsRNA-induced apoptosis in S2 cells. Z-VAD-FMK (50 μM) increases the percentage of transfected cells surviving from 26% to 63% in S2 cells. [3] Z-VAD-FMK (> 100 μM) enhances TNFα-induced neutrophil apoptosis, lower concentrations (1-30 μM) completely blocks TNFα-stimulated apoptosis in human neutrophils. [4] Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes. Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes detected with the TUNEL assay. [5]
Cell Data
Cell LinesAssay TypeConcentrationIncubation TimeFormulationActivity DescriptionPMID
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HGL5MV7BdI9xfG:|aYOgRZN{[Xl?Mor6OVDPxE1?NV76V|NWOjSqNInQUlRqdmSrY3H0[ZMh[SCycn;0[YN1cX[nIHXm[oVkfCCjZ3HpcpN1KGW2b4Dvd4ll\Q>?NHXTfJczPjF4OUC3OS=>
HepG2MXzBdI9xfG:|aYOgRZN{[Xl?NWTGbWZwOjEQvF2=MlvoNYg>NHqwdmhifHSnboXheIVlKHSqZTDhdI9xfG:2aXOgbY5lfWO2aX;uJI9nKEmLST2xNOKhMVOyOlE3PDd7NR?=
BEL-7402M1e3bGFxd3C2b4Ppd{BCe3OjeR?=M3TzeFIx|ryPMonsNYg>NVfUepFy[XS2ZX71ZZRm\CC2aHWgZZBweHSxdHnjJIlv\HWldHnvckBw\iCLSVmtNVDDqA>?NVHlS4llOjZzNkS3PVU>
CEFM3vkbGtqdmG|ZTDBd5NigQ>?NV34dlJGOCx|Mzy2O{wyODEQvF2=NXOwNoRZOTWvaX6=Mof3[I94di2{ZXf1cIF1\XNiUGKg[Y57gW2nIHHjeIl3cXS7IITvJFQx97zHIHH0JFExOM7:TR?=Mlz6NlYyODJ|M{m=
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HUVEC-2cMl7mRZBweHSxc3nzJGF{e2G7MkHwOVDPxE1?NFjZOZQ3cA>?M2qwSIRm[3KnYYPl[EB1cGVib4itUGRNNWmwZIXj[YQh[XW2b4DoZYd6Mn;RNlYxOjF5Mkm=
U1MV\BdI9xfG:|aYOgRZN{[Xl?NX3hcFFMOC1zMEFOwG0>M1nkdFJpMXHy[YR2[2W|IHTyeYcucW6mdXPl[EBieG:ydH;zbZMh[W6mIIP1ZpNmeXWnboSgTGlXNTFicnXwcIlk[XSrb36gbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK=MXeyOVk5ODl2Mh?=
ACH-2MVXBdI9xfG:|aYOgRZN{[Xl?NFHvUmcxNTJyMN88US=>MYqybC=>M1OydJJm\HWlZYOg[JJ2\y2rbnT1Z4VlKGGyb4D0c5NqeyCjbnSgd5Vje2WzdXXueEBJUVZvMTDy[ZBtcWOjdHnvckBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=>NEHsUZIzPTl6MEm0Ni=>
U1Mn[wT4lv[XOnIFHzd4F6MnvXNVAx|ryPNXvLVFRUOmh?NEHtU3RqdmirYnn0d{Bk[XOyYYPlMVM>MoX0NlU6QDB7NEK=
A549/V16M4XSTGFxd3C2b4Ppd{BCe3OjeR?=MkTnOVDPxE1?MlTaNYg>NFn6Spdz\XC{ZYPz[ZMhXEdxVF2tbY5lfWOnZDDhdI9xfG:|aYO=NVzRZnVxOjV7NE[wN|M>
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HCT116 MVPBdI9xfG:|aYOgRZN{[Xl?NWTNOlB5PTEQvF2=NUewSFZpOmh?MnXsbY5pcWKrdIOgeIhmKGOnbHyg[IVifGhiaX7keYNm\CCkeTDESHZRM4H6b|I2QDZ6OEG4
DTK-SMEM1jLZWFxd3C2b4Ppd{BCe3OjeR?=MWm1NO69VQ>?NWfJOHhHOmh?MXzEUXNQMX7wZZJ1cWGubImgbY5pcWKrdIOgeIhmKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IHL1dIl3[WOjaX7lNILyUIkzPTh2M{i5Oy=>
HL-60M2TaXWFxd3C2b4Ppd{BCe3OjeR?=M4fMTFUx|ryPNILzVos1QGh?M1XmTpJm\HWlZYOgZ4VtdCCmZXH0bEBk[XW|ZXSgZpkhW0uLLVnJJJRz\WG2bXXueOKhMXqyOVgzPDB2Mx?=
U937NYDTV5NHSXCxcITvd4l{KEG|c3H5NFTQV4Y2OM7:TR?=NHHDPIc1QGh?NGm1[ZVz\WS3Y3XzJINmdGxiZHXheIgh[2G3c3XkJIJ6KFONST3JTUB1emWjdH3lcpTDqA>?Mn3oNlU5OjRyNEO=
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HeLaMWrBdI9xfG:|aYOgRZN{[Xl?M3u2NlEx|ryPM{XvO|Q5cA>?M4PkdIRm[2yrbnXzJJRp\SC{YYTlJI9nKGGyb4D0c5NqeyCmcnHtZZRq[2GubIm=M2WwdFI2Pzd{NUS1
SGC-7901MlnSRZBweHSxc3nzJGF{e2G7MUOyNOKh|ryPMmjONYg>M4T5S4lvcGmkaYTzJJRp\SClZXzsJIRm[XSqIHnu[JVk\WRiYomgc5hidGmybHH0bY4>NGLUdnozPTd4N{C3Oi=>
MCF-7MXPBdI9xfG:|aYOgRZN{[Xl?M{[yU|Ex|ryPMlfPNog>MV7pcohq[mm2czD0bIUh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IEPCMn7ZNlU4OjJzMUS=
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Neocortical NeuronMXzO[ZVzd3SxeHnjbZR6KEG|c3H5NGq2[ZYyODEQvF2=NE[yNG4ycA>?NU\1[oF{[W62YXfvcol7\XNiSH;pZY1q\GViQT3JcoR2[2WmIF7leZJwfG:6aXPpeJk>M4DVbVI2Pjd3MECx
Primary human placental cytotrophoblastsM2raOmFxd3C2b4Ppd{BCe3OjeR?=MXmzNO69VQ>?NV3SdGx5OjSqNVnwT5RVTE2VTx?=NHnWRoVz\X[ncoPld{B1cGViaX7obYJqfGmxbjDv[kAyOc7{LVjTSFIh[nlidILpZ4xwe2GwwrC=NIf0eG0zPTZ2MkW5Ni=>
MMNUTEUGN4SXCxcITvd4l{KEG|c3H5NIXKRVA2OM7:TR?=MUSyNI1qdg>?NUfSWpFUeGG{dHz5JIlvcGmkaYTzJHNJUy2rbnT1Z4VlKCClZXzsJIRm[XSqNUC0UW9[OjV3M{CwPVg>
DLD1Mn3NRZBweHSxc3nzJGF{e2G7NX65eFlJOjEEoN88US=>MUGxbC=>MXrwZZJ1dHlicnX2[ZJ{\XNiY3XscEBieG:ydH;zbZMh[2G3c3XkJIJ6KFeVUEG=NHLPcm0zPTV{NEK0Oi=>
MDA-MB-231MYjDfZRwfG:6aXPpeJkhSXO|YYm=M3XrTFEx|ryPMnz1NYg>MnjsZZVodWWwdIOgZ4VtdCCmZXH0bEBi\nSncjDDTHVCKHS{ZXH0cYVvfA>?MnTUNlU2OjF3MEG=
MKN28Mke1RZBweHSxc3nzJGF{e2G7M{\DcVEx|ryPMUSzNI1qdg>?NIL1NmhFVVORM3v2XolvcGmkaYTzJHRPTi4QsTDwcJV{KEOKWD3pcoR2[2WmIHHwc5B1d3Orcx?=M13KZ|I2PTF|OU[w
HL-60NYHCSVBpS3m2b4TvfIlkcXS7IFHzd4F6MY[xNFDPxE1?M2HtVlFpNWHkRVN{TE2VTx?=NF[weZrDqGmvcILveoV{KH[rYXLpcIl1gSCxZjDUR25Cey22cnXheIVlKGOnbHzzNUPX[nU{OjV3MEK5N|I>
AGSMV;BdI9xfG:|aYOgRZN{[Xl?NEPofWoyOM7:TR?=NXq1b5RuOWh?NHf0bIxxemW4ZX70d{BkfXKldX3pck1qdmS3Y3XkJINt\WG4YXflJI9nKGOjc4Dhd4UuOyxiLUisJIFv\CBvOTDwdo91\Wmwcx?=Mmj4NlU1QTJ{MUS=
A549 MYXBdI9xfG:|aYOgRZN{[Xl?MUOxNO69VQ>?NYXPSZNsOjSqNWnHTWdO[myxY3vzJGROSVNvaX7keYNm\CClbHXheoFo\SCxZjDjZZNx[XOnLUOgZY5lKFCDUmCgZY5lKGGyb4D0c5Rq[yClZXzsJIRm[XSqM1fze|I2PDN2OUi5
INS-1MYnBdI9xfG:|aYOgRZN{[Xl?NYDrcJVXPTEQvF2=NWHXeIM4Pmh?NF3ydXdFVVORM4XtSIRm[3KnYYPld{B1cGVicILvdI9zfGmxbjDv[kBm[XKueTDhdI9xfG:2aXOgZ4VtdHQEoB?=MX[yOVQ{ODh7Nx?=
Primary hepatocytes MV7BdI9xfG:|aYOgRZN{[Xl?NWX5UYdyPTEQvF2=MlrLNVhpMofrbY5pcWKrdIOgZZBweHSxc3nzJI9nKGincHH0c4N6fGW|IHnu[JVk\WRiYomgRYN1KERiYX7kJHRPTi4QsR?=NXvSUpd4OjV2MEe1N|g>
L929-A MUPDfZRwfG:6aXPpeJkhSXO|YYm=Mor2NlDDqM7:TR?=MmHCNlRpMVPpcohq[mm2czDUUmbPuS2rbnT1Z4VlKGOnbHyg[IVifGh?MlHBNlU{QTh3NEC=
L929-NNEHIeI1EgXSxdH;4bYNqfHliQYPzZZk>MXuyNOKh|ryPMX[yOIg>NXywcIY4\W6qYX7j[ZMh\GWjdHigeoliKGG3dH;jdolv\SCWTldOtUBxem:mdXP0bY9vM2jLdVI2Ozl6NUSw
Cytotoxicity AssayMWfD[YxtKF[rYXLpcIl1gSCDc4PhfS=>Mo\aNlDPxE1?MlrEOFhpM2XDV5Bz\X[nboSgUWhOTC2rbnT1Z4VlKGOnbHyg[IVifGh?MoDGNlU{QTJzMU[=
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COS7NUTYS4oySXCxcITvd4l{KEG|c3H5MXuxNO69VQ>?M4DMSFQ5cA>?M2HIPZBienSrYXzsfUBxemW4ZX70[YQhTkNzMEGtbY5lfWOnZDDj[YxtKGSnYYToNF\6UI8zPTN6NECyOS=>
A375M1LFfWFxd3C2b4Ppd{BCe3OjeR?=M{XX[VMx|ryPNYCxZXU2Omh?MoTadJJmfmWwdIOgeIhmKGS{dXetbY5lfWOnZDDQRXJRKGOuZXH2ZYdmNVHoemVsOjV|N{[xNVU>
A549NIPiSGtCeG:ydH;zbZMhSXO|YYm=NVjVXYtWPTEQvF2=NYfxRY9qOjSqNYP5Z5FkemW4ZYLz[ZMhemmkb4PvcYUh[mmxZ3Xu[ZNqeyCjbnSgZZBweHSxc3nzJINifXOnZDDifUAhS2iran;u[4Ridg>?MVSyOVM1QTd6MR?=
A549M1LS[GFxd3C2b4Ppd{BCe3OjeR?=MoLMNk42NTJ3zszNM4PyXlFpMoXG[IVkemWjc3XzJJRp\SCyb4D1cIF1cW:wIH;mJIFxd3C2b4TpZ{Bk\WyuczDk[ZBmdmRib36gZ49v[2WwdILheIlwdnN?NIPORpIzPTN2MkSyOy=>
 NBL-W-S NX\LTWpiSXCxcITvd4l{KEG|c3H5M3zLTFUx|ryPNIXyeWYycA>?M3PiToZ2dGy7IILld4N2\XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgS2FPXC14MTD0doVifG2nboS=MWCyOVMzOzJ{Mh?=
Caki-1 MkjzRZBweHSxc3nzJGF{e2G7M2T3UFQx|ryPNFr6OnI1QGh?MXvy[YR2[2W|IITo[UBvfW2kZYKgc4YhSW6wZYjpckBXNXCxc3n0bZZmKGOnbHzzNWLDflhLOjV{N{mxPVE>
769-PMXHBdI9xfG:|aYOgRZN{[Xl?NFqxV5k1OM7:TR?=NXvScGo5PDiqNFT5cWNz\WS3Y3XzJJRp\SCwdX3i[ZIhd2ZiQX7u[ZhqdiCYLYDvd4l1cX[nIHPlcIx{MnrENlUzPzlzOUG=
H9c2NG\mXIFCeG:ydH;zbZMhSXO|YYm=MkLHOVDPxE1?MlXWNYg>MnHzbY5pcWKrdIOgSG9ZNWmwZIXj[YQh[2G|cHHz[UA{KGGldHn2ZZRqd25iYoX0JI5wfCC2aHWgcI9{eyCxZjDj[Yxtew>?MX:yOVI5OzhzOR?=
K562NXXwdXY6SXCxcITvd4l{KEG|c3H5MXq1NO69VQ>?NF7iWGg1cA>?NILkXppqdmirYnn0d{BL[WNvQT3pcoR2[2WmIHPlcIwh[XCxcITvd4l{M3fVXFI2OjRzNkG5
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A549MV3BdI9xfG:|aYOgRZN{[Xl?NV\K[W1tOTEQvF2=MmnKN4g>M2jxUJJm\HWlZYOgZ4VtdCCjcH;weI9{cXNiY3H1d4VlKGK7IGDRVS=>MmqzNlUyPjF4OUm=
MCF-7M33RbmFxd3C2b4Ppd{BCe3OjeR?=NFn1S4wzOM7:TR?=NVexU3hFOmh?NGDxUGRl\WO{ZXHz[ZMhU0SULYPpVm5CNWmwZIXj[YQh[XCxcITvd4l{M4PtbVI2OTh{MkSw
OSM4TVVWFxd3C2b4Ppd{BCe3OjeR?=M3PjW|IxNzRyzszNNWT4SXRjPzKqNGT6OYdqdmirYnn0d{BQWyClZXzsJJZq[WKrbHn0fUBz\WS3Y4Tpc44h[nliQ{[gZ4Vz[W2rZHW=NV3QTZBrOjVzNUKzPVk>
HUVECsM2PTcGFxd3C2b4Ppd{BCe3OjeR?=M2G1NVEx|ryPMXiybC=>M1noWuKhcW6qaXLpeJMh[XCxcITvd4l{KGGwZDDmZYNqdGm2YYTld{BifXSxcHjh[5khcW5iRFXOWlIucW6oZXP0[YQhUFWYRVPzNYiy[I1SOjVzM{i3NFM>
EA.hy926NIfqRZNCeG:ydH;zbZMhSXO|YYm=M2WzfFEx|ryPMYSybC=>NX2xWmlxyqCrbnjpZol1eyCjcH;weI9{cXNiYX7kJIZi[2muaYTheIV{KGG3dH;wbIFogSCrbjDESW5XOi2rbn\lZ5Rm\CCHQT7ofVkzPg>?MofNNlUyOzh5MEO=
EbsM2SyZ2Fxd3C2b4Ppd{BCe3OjeR?=NFG4fWMyOC1zMEFOwG0>M2HXclI1cA>?NHSyc4nDqE2PVGOg[4Vv\XKjdHnvckBz[XSnIHTlZ5Jm[XOnZDDhd{B1cGViY3;uZ4VvfHKjdHnvckBw\iC8LW\BSE5ndWtiaX7jdoVie2WmNWnnd4p7OjVzM{S4NVc>
JurkatNGDmUXZCeG:ydH;zbZMhSXO|YYm=M{fzNlIx|ryPMne5NlRpMV\EUXNQNX[yZ5FQeGG{dHnhcIx6KGmwaHnibZQh[2WubDDk[YF1cCCxZjDKeZJs[XRiY3XscJMhcW6mdXPl[EBjgSBzMEC1PE1HPCClb33ibY5m\CC5aYToJHZRScLiNIj3VmwzPTF{MEeyNy=>
K562NX7hdFFGSXCxcITvd4l{KEG|c3H5NVzFb|JHOC5zLUJOwG0>MWCxbC=>NXrpPYJ6cW6qaXLpeJMh[2ynYY\h[4Uhd2ZiSGPQPVAhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI>NYHQepJzOjVzMUmxPFg>
IMR-32MYLBdI9xfG:|aYOgRZN{[Xl?NILpZ3U1OMLizszNxsA>NXXXTWkzOmh?Mnj2[IVkemWjc3WgbY4h[XCxcITveIlkKGOnbHzzJINwdXCjcnXkJJRwKFRvMjD0c5hqdg>?M4TtdVI2ODh2N{W1
RPMI 8226MorwRZBweHSxc3nzJGF{e2G7NXfP[WNIOTByzszNMorLNYg>M3XT[IFtdW:|dDDjc41xdGW2ZXz5JIJtd2OtczDj[YxtKGSnYYToJINifXOnZDDifUBv\WO{b4P0ZZRqdi1zM37zOlI2ODN5OEWx
U266 MXLBdI9xfG:|aYOgRZN{[Xl?M4nSVlExOM7:TR?=Ml;nNYg>MXnwZZJ1cWGubImgZoxw[2u|IHPlcIwh\GWjdHigZ4F2e2WmIHL5JI5m[3Kxc4TheIlvNTF?NHj6UHczPTB|N{i1NS=>
H929 MnLwRZBweHSxc3nzJGF{e2G7MUmxNFDPxE1?Mlf3NYg>MVPwZZJ1cWGubImgZoxw[2u|IHPlcIwh\GWjdHigZ4F2e2WmIHL5JI5m[3Kxc4TheIlvNTF?NVq0d2UxOjVyM{e4OVE>
MM.1S MVnBdI9xfG:|aYOgRZN{[Xl?MUmxNFDPxE1?NGO4NlUycA>?MmrL[YZncWOrZX70cJkheHKndnXueJMhe2:{YX\lcoljNWmwZIXj[YQh[2WubDDk[YF1cCClb33ibY5mKHerdHigcoVkem:|dHH0bY4uOQ>?NYT1d2xrOjVyM{e4OVE>
C6NXvTd41lSXCxcITvd4l{KEG|c3H5M2rCbVUx|ryPMnHBOFhpNGLLTYlxemW4ZX70d{B1cGVibH;zd{Bw\iClZXzsJJZq[WKrbHn0fUBk[XW|ZXSgZpkheHKnZ37lco9td26nMnLnNlUxOTN2N{m=
AGSM2TZNWFxd3C2b4Ppd{BCe3OjeR?=NIm1VlQ2OM7:TR?=MnzKNlRpNUj4dodo[WKxbHnzbJMh|rJvbHHwZYNpd26nLXnu[JVk\WRiY3XscEBl\WG2aDDhcoQhcW6qaXLpeIVlKGe{b4f0bC=>NHm1cm0zPTByOU[5PC=>
HeLa NEjQNY5CeG:ydH;zbZMhSXO|YYm=MnHtOFDPxE1?M2fJeFI1cA>?NHf0N4hqdmirYnn0d{B1cGViaX7jdoVie2WmIHHwc5B1d3OrczDpcoR2[2WmIHL5JJNqWk6IMUKxMlHxNlQ6Ojh4OEW=
THP-1 MWHBdI9xfG:|aYOgRZN{[Xl?M3P4Z|Ex|ryPNYnVcZA2OWh?NIGxWFNz\WS3Y3XzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEGOQT3TSHTDqA>?NFjwO44zPDl{M{[1Ny=>
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fetal rat lung fibroblasts MoLKT4lv[XOnIFHzd4F6MmnFPFDPxE4EoB?=M1fsdFFpM3vGVolvcGmkaYTzJGNie3Cjc3WtN{Bi[3Srdnn0feKhM3HZ[VI1OzF2MUO1
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THP-1 M4SyTmFxd3C2b4Ppd{BCe3OjeR?=M4\oNlExNTVyzszNMV[ybC=>MXXpcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUB1emmydH;sbYRmM1nPOlI{QTByMkm5
K562MkDmRZBweHSxc3nzJGF{e2G7NUDVbpNoOjEQvF2=MVK0PIg>NEX6T2dqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSB2LV3VM1LCSVI{QDd4OEK2
HL-60MVLBdI9xfG:|aYOgRZN{[Xl?NVS4OpZROTByzszNNG\a[WIzPGh?NWnkNnlTcW6qaXLpeJMh[XCxcITvd4l{KGmwZIXj[YQh[nliYXLp[ZRidmViZHn0[ZJx\W6nc9MgNF\nVokzOzh4NUe3PC=>
HeLa M3XJ[WFxd3C2b4Ppd{BCe3OjeR?=MVGyNO69VQ>?MVyybC=>M4rhWIlvcGmkaYTzJGROVVBiaX7keYNm\CCjcH;weI9{cXN?NUjtN|lZOjN6NkO5OlY>
U251NGjuV21CeG:ydH;zbZMhSXO|YYm=MW[yNO69VQ>?MnO3NlRpNV3se|BQcW6qaXLpeJMh[2G|dHnjbY4hcW6mdXPl[EBIOi:PIIDoZZNmKGG{cnXzeEBidmRiYYDvdJRwe2m|MXqyN|gyPjhzNh?=
HL-60MVPBdI9xfG:|aYOgRZN{[Xl?M2DtOFUxyrWPMknvOIg>MXPicI9kc3NidHjlJINt\WG4YXflJI9nKGOjc4Dhd4UuOyxiLUmsJIFv\CCSQWLQJIlv\HWlZXSgZpkhPnN?NEjDbYYzOzhyNEewOi=>
Ec-109MnqwRZBweHSxc3nzJGF{e2G7NXfHNnRjOTBizszNM4PkT|Q5cA>?NYXYTHMxemWycnXzd4V{KFCHLX3l[IlifGWmIFXjMVExQSClZXzsJIFxd3C2b4Ppdy=>MVKyN|c5OjZ2MR?=
RKOMoK3R4VtdCCYaXHibYxqfHliQYPzZZk>MVixNEDPxE1?M1vMU|I1cA>?NWjON3N4cW6qaXLpeJMhfGinIHTlZ5Jm[XOnIH;mJINmdGxidnnhZoltcXS7IHPheZNm\CCkeTDERWI>NHTzboIzOzd3OEC2OC=>
T98GNH;JOGZE\WyuIG\pZYJqdGm2eTDBd5NigQ>?NHX0SHoyNTFyMN88US=>NIG0fngzPGh?NVjzcYVbcW2ycn;2[ZMh[2WubDD2bYFjcWyrdImgZ491emWjdH3lcpQhf2m2aDDUVy=>MkDFNlM4Pjl{N{W=
JurkatMUTD[YxtKF[rYXLpcIl1gSCDc4PhfS=>MlfRNVAxNTJyMN88US=>MYiyOIg>NY\EV5VGcW6qaXLpeJMhUGGDNDDpcoR2[2W|IHHwc5B1d3OrczDk[ZBmdmSnboSgc4Yh[2:wY3XueJJifGmxbtMgMX[yN|c{OjR6MR?=
Molt-3 M3W4bmFxd3C2b4Ppd{BCe3OjeR?=Ml;IOVDPxE1?MYWybC=>MlzIdoVlfWOnczDt[YxifG:waX6tbY5lfWOnZDDhdI9xfG:|aYO=MnHsNlM4OjVyMUO=
hMSC12M4G0fWFxd3C2b4Ppd{BCe3OjeR?=MUKxNFDPxE1?M2rZS|RlM4jVXolvcGmkaYTzJI9{fGWxZ3XubYMh[3WudIXy[U1qdmS3Y3XkJINmdGxiZHXheIgh[W6mIHPhcINq\mmlYYTpc44>NUG2N|R5OjN4NUe4NlI>
HM7 MmT4RZBweHSxc3nzJGF{e2G7NIDOe3QzOM7:TdMgNH3UfXMycA>?MWHicI9kc3NiYYDpZ5Vt[XKnbjDBJIFk\XSjdHWtbY5lfWOnZDDjZZNx[XOnLUOgZYN1cX[jdHnvckBidmRiUFHSVEBkdGWjdnHn[S=>NGHtOXEzOzV6M{SxNi=>
Hep-2 M3OzXWFxd3C2b4Ppd{BCe3OjeR?=MVSyNO69VcLiNF\tVZczPGh?MWPEUXNQM3y2cYFtdGW4aXH0[ZMhfGinIHTlZ5Jm[XOnIH;mJINmdGxidnnhZoltcXS7IHnu[JVk\WRiYomgd4ltcWKrbnnuNIjEWGMzOzV6MECzNi=>
HSCsNVPwdWJKSXCxcITvd4l{KEG|c3H5M1y1RVUx|ryPNIi0[mMzPGh?NXfnOIh3TE2VTx?=NHu1ZZRqdmirYnn0d{BvcWyxdHnubYIucW6mdXPl[EBFVkFiZHHtZYdmKGmwZHnjZZRm\CCkeTDQRXJRKGOuZXH2ZYdmMXmyN|Q6QTh5NB?=
HL-60 Mlr2R5l1d3SxeHnjbZR6KEG|c3H5M2PMc|Ix|ryPwrC=MlXLNlRpM2DWVZJm\HWlZYOgeIhmKGO7dH;0c5hq[yCnZn\lZ5Qhd2ZiRYL5OS=>NVfSNY1nOjN2OUS0PFA>
HAMmTORZBweHSxc3nzJGF{e2G7M2fV[FUx|ryPMmjZNlRpNFiwN2J{fXCycnXzd4V{KHSqZTDjcIVifmGpZTDv[kBRSVKSIHHu[EBk[XOyYYPlMVMtKC15LDDhcoQhNTliaX7keYNm\CCkeTDic5J1\XqxbXniM3NCUEF?M37a[|I{PDd3OUW2
C666-1MlfERZBweHSxc3nzJGF{e2G7NEK5U2o2OM7:TR?=NUL4O2hROjSqNXTYcItqe3WycILld5NmeyC2aHWgZ4xm[X[jZ3Wgc4YhWEGUUDDhcoQh[2G|cHHz[U0{NCBvNzygZY5lKC17IHnu[JVk\WRiYomgZo9zfGW8b33pZk9USUiDM4LpZlI{PDd3OUW2
Hepa1-6NGT3VHVCeG:ydH;zbZMhSXO|YYm=M1PDeFUx|ryPNUPlS3gzOmh?NYT0To1bcW6qaXLpeJMh[3W{Y4XtbY4h[W6mIILld5ZmemG2cn;sMYlv\HWlZXSgZZBweHSxc3nzNUDmcHVPOjN2NE[3OVM>
PLC/PRF/5cM1vEVGFxd3C2b4Ppd{BCe3OjeR?=NVjhV5FOPTEQvF2=M1[1OlFpNVjyO4V5eHKndnXueJMh[XCxcITvd4l{KHS{aXfn[ZJm\CCkeTDDTW4>M3zSXVI{PDN6OEK0
HCT116NYPIfldDSXCxcITvd4l{KEG|c3H5MnnxNVAx|ryPNWWwc|JFOS53aB?=M1XzXYlvcGmkaYTzJG9{NWmwZIXj[YQh[2WubDDk[YF1cA>?NGj3SIgzOzd2NEO1Ny=>
HCECMX;GeY5kfGmxbjDBd5NigQ>?NInNd3Y2OM7:TR?=M1W4SFczcA>?NX25[YRkTE2VTx?=NY\CdnV7emW|dH;y[ZMhd2ZidHjlJI5wem2jbDDIR2VEKHCqZX7veJlx\Q>?M4L3eFI{PzR{MEGx
Primary rat cerebral cortical neuronsNHn6fG1CeG:ydH;zbZMhSXO|YYm=NUHITFFuOTByzszNNFvFVmcycA>?M{DhU5Bz\X[nboTzJGNlNWmwZIXj[YQh[XCxcITvd4l{KGGwZDDj[YxtKGSnYYToNVLxRYpNOjN5NEGzNVc>
MDA-MB-231 NYLEcZZtSXCxcITvd4l{KEG|c3H5NXW5R4s1OjYQvF2=NGrxRoszPGh?M2PkbYFjem:pYYTld{BkgXSxdH;4bYNqfHliYX7kJINt\WG4YXflJI9nKGOjc4Dhd4UuOyCjbnSgVGFTWCCrbnT1Z4VlKGK7IGLnNy=>MYSyOVM{PzV2NB?=
NLRP3-Tet-on-MC/9 NXm4WVhpSXCxcITvd4l{KEG|c3H5MmXZNVAuPDEQvF2=Mnj1NVJpMn34ZZR1\W63YYTld{B1cGViY3XscEBl\WG2aDDjZZV{\WRiYomgR2FRWy2jc4PvZ4lifGWmIF7MVnA{KG23dHHueJMhcW5idHjlJHRmfC2xbjDzfZN1\W1?NXPt[VNnOjN5MEOzPFk>
KNS42 MnWxRZBweHSxc3nzJGF{e2G7NVPVUXBMPTEQvF2=MoG4NYg>MnradoVlfWOnczDj[YxtKGSnYYToJIFv\CClb33wcIV1\Wy7IHHic4xqe2inZDDjZZNx[XOnIEOvO{Bi[3Srdnn0fUBqdiC{ZYPwc45{\SC2bzDBRnQuOjZ|L{LES{9u\XSob4LtbY4h[2:vYnnuZZRqd25?NW\qcoN[OjN4OUGxOFU>
MCF-7 NYHQXnFQSXCxcITvd4l{KEG|c3H5M{L1VlIx|ryPwrC=MXq3Nog>M2e4bolvcGmkaYTzJIVyfW:uLTDhcoQhPC2RSGStcYVlcWG2ZXSgZZBweHSxc3nzNUXEW2pXOjN4N{W2OFM>
hCMEC/D3 NY\hOo5NSXCxcITvd4l{KEG|c3H5NG\MVFQzPc7:TR?=NI\2[o04Omh?NUPJbHZHemWmdXPld{BNfHiDIHnu[JVk\WRiYYDvdJRwe2m|NYPuWGhXOjN4NkWxPVg>
JurkatM2HTeWFxd3C2b4Ppd{BCe3OjeR?=MXixNk42NTVyzszNNY\G[mNwOWh?NWrxNXpv\G:|ZTDk[ZBmdmSnboTsfUB{fXCycnXzd4V{KFOSSD3pcoR2[2WmIGDhdk01KGOuZXH2ZYdmNCCSQWLQJINt\WG4YXflMEBFVkFiZoLh[41mdnSjdHnvckwh[W6mIHzvd5Mhd2ZidnnhZoltcXS7M33Mb|I{PDR{OUe2
CNE-1MorsRZBweHSxc3nzJGF{e2G7M2DpU|Ix|ryPwrC=M2jNXVI1cA>?Ml\sbY5pcWKrdIOgVmFFODBzLXnu[JVk\WRiY3XscEBl\WG2aB?=NYrw[lNnOjN2Mk[4OVA>
HONE-1NHnieWZCeG:ydH;zbZMhSXO|YYm=NFfWW2wzOM7:TdMgMlX1NlRpMkW1bY5pcWKrdIOgVmFFODBzLXnu[JVk\WRiY3XscEBl\WG2aB?=NYjkUXNtOjN2Mk[4OVA>
astrocytes cellM1TkZmFxd3C2b4Ppd{BCe3OjeR?=NXvMZWhsPDEQvF2=NV7OTWRZPmh?NVvjPGpremWmdXPld{Bm[XKueTDhdI9xfG:|aYOgbY5lfWOnZDDifUB{fGG3cn;zdI9zcW6nM{Pv[VI{PDFzN{e4
U-937MmLWRZBweHSxc3nzJGF{e2G7NEnSflIyOM7:TR?=NH;ifGk{OG2rbh?=NYPvUFNkeHKndnXueJMhXE6ILXnu[JVk\WRibnXjdo9xfG:|aYO=NETVeHozOzRzMEe0PC=>
MDA-MB-231MVvBdI9xfG:|aYOgRZN{[Xl?NHHrPIgyODEQvF2=MkHsNYg>NW\RXGVKcW6qaXLpeJMhe2Wwc3n0bZpifGmxbjD0c{BVWkGLTDD1dI9vKE2WRFig[I94di2{ZXf1cIF1cW:wMYGyN|QxQDR{OR?=
HeLa MkLqRZBweHSxc3nzJGF{e2G7NWjad3Q4OTByzszNNFSyU|QzcA>?Mmq5Zoxw[2u|IFrSV{0yPSCLbnT1Z4V{KEGyb4D0c5Rq[yCFZXzsJGRm[XSqNH:xfGYzOzN2NEC0OS=>
Ec109NILHRYZCeG:ydH;zbZMhSXO|YYm=MVyxNO69VQ>?M{fvdlZpMl7JZoxw[2u|IHHwc5B1d3SrYzDjc41jcW6jdHnvckBw\iCWYYStV41i[055IHHu[EBz[WSrYYTpc44>Ml;1NlM{Ozh3Nki=
H460 MU\BdI9xfG:|aYOgRZN{[Xl?MofRNVDPxE1?M{\kPVZpNX7YdG5X[myxY3vzJIFxd3C2b4TpZ{Bkd22kaX7heIlwdiCxZjDUZZQuW22jY163JIFv\CC{YXTpZZRqd25?NIDDXVAzOzN|OEW2PC=>
HeLa M1jjTWFxd3C2b4Ppd{BCe3OjeR?=MlzBOVDPxE1?MV:xMlVpMV;hZpJw\2G2ZYRCpGNpdGGveXTpZU1qdmS3Y3XkJIFxd3C2b4Ppdy=>MUiyN|MxOzhyNB?=
SK-HEP1M{i1T2Fxd3C2b4Ppd{BCe3OjeR?=MVSxNFDPxE1?MmfGNYg>NUXBfGlycW6qaXLpeJMhS3KWMTDhZ5RqfmG2ZXSgZ4F{eGG|ZT2zMEAuPyxiLUisJE06NCCjbnSgdI9tgSiDRGCtdoljd3OnKTDwc4x6dWW{YYPlNVruVHVCOjN|MEK2OVA>
QGY7701NV;qWZJWSXCxcITvd4l{KEG|c3H5M1XJdVI2|ryPMkfsNU42cA>?NUnySVNocW6qaXLpeJMh[WOldX31cIF1cW:wIH;mJJN2[i2JMTDwbIF{\SCrbnT1Z4VlKGK7IFTPXEAsKHG3ZYLj[ZRqdg>?M33Yb|I{OjRyME[x
HepG2M3v5NWFxd3C2b4Ppd{BCe3OjeR?=MV2yNO69VQ>?MV:zNI1qdg>?M{HhTolvcGmkaYTzJJRp\SCnbnjhcoNm\CClZXzsJIRm[XSqIHL5JINwdWKrbnXkJJRz\WG2bXXueEBw\iCjcHnn[Y5qdiCjbnSgWHJCUUx?NUL4RYpQOjN{MkSyN|k>
U87 NV\w[ZZ1SXCxcITvd4l{KEG|c3H5M4n3e|I2|ryPMYWybC=>NUn0XZpS\GWlcnXhd4V{KGm|b3zpdZVqemm2aXflcolvKCiLU1ypMYlv\HWlZXSgZZBweHSxdHnjJINmdGxiZHXheIgtKGK3dDDuc5QhdmWlcn;0bYMh[2WubDDk[YF1cA>?M2G0SFI{OjJ7NkK2
HSC-2NW\OfpdwSXCxcITvd4l{KEG|c3H5M{\JTVI2NzVyzszNNVXJOHRNOWh?MYPpcohq[mm2czDTUk0{QC2rbnT1Z4VlKGO7dH;0c5hq[2m2eR?=MXGyN|E2PTJ2OB?=
HSC-4Mn3JRZBweHSxc3nzJGF{e2G7NGPEeJozPS93MN88US=>MlrJNYg>MXnpcohq[mm2czDTUk0{QC2rbnT1Z4VlKGO7dH;0c5hq[2m2eR?=NHLBXYgzOzF3NUK0PC=>
CL-1NX;vW2VXSXCxcITvd4l{KEG|c3H5MUiyNO69VQ>?NFXubIoycA>?M3vhWIJtd2OtczDPVFQ1QSCrbnT1Z4VlKGOnbHyg[IVifGh?NFfv[ZczOzF|MUe4Ni=>
MELNVfxNHB5SXCxcITvd4l{KEG|c3H5MUWyNO69VQ>?MonjNlRpM1fl[GROW09?NXzVe4ZNcW2yYXny[ZMhWDK[Nz3pcoR2[2WmIF3FUEBk\WyuIHHwc5B1d3Orcx?=M{PtPFI{ODF2OEi3
Bel-7402 Mo\sR4F{eGG|ZTDBZ5RqfmG2aX;uJGF{e2G7MUS1NO69VQ>?NIn0SFEzcA>?NEPlNIRqdmirYnn0d{BRS0VvaX7keYNm\CCjbn;pb4l{NGLzRoMzOzByOEe0Ni=>
Eca-109M3jXbWFxd3C2b4Ppd{BCe3OjeR?=NXXrdG9mOjYQvF2=MXGzNI1qdg>?MU\pcohq[mm2czDCTk1DOTFvaX7keYNm\CClZXzsJIRm[XSqMVSyN|A4Pjl4Nx?=
MELM2q0emFxd3C2b4Ppd{BCe3OjeR?=MY[yNO69VQ>?NGTQTpoycA>?NH7qdJlFVVORNYPTN4oycW2yYXny[ZMhWDK[Nz3pcoR2[2WmIF3FUEBk\WyuIHHwc5B1d3Orcx?=MUKyN|AyPDh6Nx?=
Bel-7402 M1XncGFxd3C2b4Ppd{BCe3OjeR?=NUfQXWVSPTEQvF2=NUDnc5ViOmh?NV\hXXNJcW6qaXLpeJMhWEOHLXnu[JVk\WRiYX7vbYtqew>?M2\4WlI{ODB6N{Sy
L929MYjGeY5kfGmxbjDBd5NigQ>?MU[yMlXPxE1?Mo\LNYg>M{DjVIlv[3KnYYPld{BTUVBzIHX4dJJme3Orb36gZY5lKGW6YXPldoJifGWmIGTOSu6yNWmwZIXj[YQhdWm2b3Poc45lemmjbDDkfZNnfW6ldHnvckBidmRiUl;TJJBzd2S3Y4Tpc44>MYeyN|AxODVzOB?=
RCC MUjD[YxtKF[rYXLpcIl1gSCDc4PhfS=>M3\3TVExOM7:TR?=MVeyOIg>M1HhWZJm[2:4ZYLld{B1cGVidnnhZoltcXS7IH;mJINmdGy|IHX4dI9{\WRidH:gNVVlNVCJSkK=MlnLNlI6QTF2OUS=
NB2a/d1M1myN2Fxd3C2b4Ppd{BCe3OjeR?=NGm1WIMyODEQvF2=MV:3Nog>NXTt[phR[XS2ZX71ZZRmeyC|dHH1do9{eG:{aX7lMYlv\HWlZXSgZ4F{eGG|ZTDhZ5Rqfmm2eTygVGFTWCxiYX7kJJRifSClbHXheoFo\Q>?M2fqOlIzQTh6NUSx
T cellM3fJVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M13iXVI2NTFyMN88US=>NGrFUXgzPGh?NGfweplld3OnLXTldIVv\GWwdHz5JIlvcGmkaYTl[EBVKGOnbHygdJJwdGmoZYLheIlwdiCvZXTpZZRm\CC2aILveYdpKHSqZTDjc{1{fGmvdXzheIlwdiC5aYToJIFvfGlvQ1SzJIFv\CCjboTpMWNFOjh?MXKyNlk5OjV|OB?=
K562NXrnNXBkSXCxcITvd4l{KEG|c3H5NHTkU|YyODEQvF2=MlzhNYg>MmixZoxw[2u|IFHico9j[X[rc3P1ceKhTi2rbnT1Z4VlKGGyb4D0c5Nqew>?NUXQWmJHOjJ7N{KzO|I>
Jurkat MVfBdI9xfG:|aYOgRZN{[Xl?M{ntOVQx|ryPMkfQNYg>M2fufYFjd2yrc3jld{BH[XOOLXnu[JVk\WRiY3HzdIF{\SCjY4TpeoF1cW:wIHHu[EBk\WyuIHTlZZRpMljzNlI6PDJ5M{i=
BGC-823M4Wx[WFxd3C2b4Ppd{BCe3OjeR?=NHLNRVcyODEQvF2=NGHST5MycA>?NYCzXVFQeGG{dHnhcIx6KHKnc3P1[ZMh[2WubIOgZYdicW6|dDDkZY1i\2Vib3[g[IFq\HqnaX6=MnOzNlI6OjZ3NEW=
Hep3BMoSxRZBweHSxc3nzJGF{e2G7MoHVOVDPxE1?NHf1VJMycA>?NUfZe482[myxY3vzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEiHR1PzMYGyNlkzOzF3NB?=
LLC-PK1Mk\IRZBweHSxc3nzJGF{e2G7M1jBT|Ix|ryPNFO5UYQycA>?NVGwZ2dteHKndnXueJMh[2m|cHzheIlvNWmwZIXj[YQh\GWpcnHkZZRqd25ib3[gRZRoPSxiYnXjcIlvNTFuIHHu[EBCfGdzMjDwdo91\Wmwcx?=NUDaNJZROjJ6OU[wN|c>
A549M2PicWFxd3C2b4Ppd{BCe3OjeR?=NYSwSJNvPTEQvF2=NVjOSJNIOWh?NFPMOGZjdG:la4OgeIhmKEKDST3pcoR2[2WmIHHwc5B1d3Orc9MgM4TxdVIzQDh5MkG1
SGC-7901 NGThTHRCeG:ydH;zbZMhSXO|YYm=Ml3mNVDPxE1?MmfVNlRpNX3BemhHcW6qaXLpeJMh|rJuzsKt[Ilu\XSqeXzhZ5J6dHOqaXvvcolvNWmwZIXj[YQh[XCxcITvd4l{yqB?MXKyNlg1QDV7Nx?=
DM6MV7BdI9xfG:|aYOgRZN{[Xl?NFXkfVgyODEQvF2=NGXnUoY4Omh?M1m3ToJtd2OtczDic5RpKEV{Rj2xJIFv\CCHMl\0dk1u\WSrYYTl[EBkgXSxdH;4bYNqfHl?MWmyNlgzPTN{OB?=
MCF-7, MDA-MB-468, Caco-2MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NFfIRnQ2OM7:TR?=Ml;VOFhpNH72cItqdmirYnn0d{B1cGViY3XscEBoem:5dHigbY5pcWKrdHnvckBqdmS3Y3XkJIJ6KHOjcH;ubY4>MWWyNlgxODl4OB?=
A2750 NH\FOZZCeG:ydH;zbZMhSXO|YYm=NXHHbGd1OjEQvF2=NXnhZ|FrOmh?MnzQSG1UVw>?NXTGWJBl[myxY3vzJINie3Cjc3WgZ4xm[X[jZ3Wg[JVzcW6pIHjlcIVv[WyrbjD0doVifG2nboSgZY5lKHKnZIXj[ZMh[XW2b4DoZYdq[yClZXzsJIRm[XSqM3OzSlIzPzh2M{[z
U87 MlzyRZBweHSxc3nzJGF{e2G7MoO2NlDPxE1?MYOyOIg>M1;YPZJm\HWlZYOgeIhmKGGyb4D0c5NqeyC{YYTlJIlv\HWlZXSgZpkhWEyDQh?=NInl[pgzOjd5OEe4NC=>
HT1080MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MUi1NO69VQ>?M4[0U|dlNITGSpRqdmirYnn0d{B1cGViY3XscEBoem:5dHigbY5pcWKrdHnvckBk[XW|ZXSgZpkh[2:vYnnu[YQhfHKnYYTt[Y51KG:oIFTDRUBidmRiT13QMkDYNlI4PDB7OES=
A549 NVr5SWN[SXCxcITvd4l{KEG|c3H5NVjHZ2VnPTEQvF2=M2j3dlJpNUHDb4lzeGG{dHnhcIx6KGSnY4LlZZNmeyC|b3TpeY0he2WuZX7peIUucW6mdXPl[EBieG:ydH;zbZM>Ml7BNlI4OjF6MES=
Primary OPCM2HWe2Fxd3C2b4Ppd{BCe3OjeR?=NFPZXmwy|ryPMU[2bE8zPGh?MnTZdoVlfWOnczD0bIUheGW{Y3XueIFo\SCxZjDj[YxteyCrbjDlZZJtgS1iYX7kJIxifGVvYYDvdJRwe2m|L37lZ5Jwe2m|MVOyNlcxPzN6NR?=
PMNsMWnBdI9xfG:|aYOgRZN{[Xl?NXi0T|Z{PDEQvF2=MX62bC=>NVTjVFZsTE2VTx?=MlfjdoV3\XK|ZXSgeIhmKGGvb4XueEBw\iClbHXheoVlKGOjc4Dhd4UuOyC2bzDu[YFzKH[naHnjcIUhdGW4ZXzzMYKyNlY6OjV5Nx?=
A549 M4W0UmFxd3C2b4Ppd{BCe3OjeR?=NEnwTWw2OM7:TR?=Mk\RNYg>NXG3XGc4eHKndnXueJMh[XCxcITvd4l{KGmwZIXj[YQh[nliQnHvbJVwe2mmZTDJNUjmOXdHOjJ4OEe2N|U>
AGSMWTBdI9xfG:|aYOgRZN{[Xl?NF\CNXczOM7:TR?=M2jYTlEzcA>?NH;rSphqdmirYnn0d{B1cGViYXP0bZZifGmxbjDv[kBxem9vY3HzdIF{\S1|IHnuJJJme3CxboPlJJRwKHSqZTDFeG9C[yCocnHjeIlwdg>?MUSyNlY5PzN7OB?=
shC9 NImxXm9CeG:ydH;zbZMhSXO|YYm=Mn7QNVDPxE1?M3H2TVE3cA>?NUTscZZQ[my3boTzJHNJUCCneIDy[ZN{cW:wIHnuJJNpSzliY3XscJMh\Xiyb4Pl[EB1dyCnaYTo[ZIhWEFib4KgUHBENFTUNFIzOjZ2MUC5OC=>
primary MEFsMUDGeY5kfGmxbjDBd5NigQ>?MmO5NVJpMVxCpIlv[3KnYYPld{BmfG:yb4Pp[IUucW6mdXPl[EBucXSxY3jvcoRzcWGuIH3lcYJz[W6nIHTldI9t[XKrenH0bY9vNETYT4kzOjZzM{e2Oy=>
3T9 MEFsM4DCRmZ2dmO2aX;uJGF{e2G7NEjTPIkyPmh?NV20fmt[cW6lcnXhd4V{KGO7dH;jbJJwdWViYzDy[Yxm[XOnIHHmeIVzKGW2b4Dvd4ll\S22cnXheI1mdnR?MlTyNlI3OTN5Nke=
3T9 MEFsMW\GeY5kfGmxbjDBd5NigQ>?M4TUPFE5cA>?NX[xPIVxfXC{ZXf1cIF1\XNiaX7peIlifG:{IHPhd5Bie2VvOTDieZQh\G:5boLl[5Vt[XSnczDl[oZm[3SxcjDjZZNx[XOnczDh[pRmeiCndH;wc5Nq\GVidILlZZRu\W62NIrxRVkzOjZzM{e2Oy=>
MDA-MB-231 Mnv0RZBweHSxc3nzJGF{e2G7MVuyNO69VQ>?MU[0[C=>Ml;0doVlfWOnczD0bIUh[2WubDDhdI9xfG:|aYOgbY5lfWOnZDDifUBu\XSob4LtbY4hfHKnYYTt[Y51KHejczDzbYdvcW[rY3HueIx6MWGyNlU6OzR2MR?=
C6 MVXBdI9xfG:|aYOgRZN{[Xl?MoPINVDPxE1?NYnpb|hkOjSqNUnJcW1I[myxY3vzJJRp\SC|dYDwdoV{e2m4ZTDl[oZm[3Rib3[geIhmKHCncITp[IUhd25idnnhZoltcXS7Ml61NlI2QDh7OEC=
HL-60 NH\ve3dCeG:ydH;zbZMhSXO|YYm=MVixNFDPxE1?Mke3NlRpNYHyZYVlcW6qaXLpeJMhfGinIHPlcIwh[XCxcITvd4l{KHOnbHXjeIVlKChtKT3t[Y51cHmuIN8yMUgy6obUNjmt[IlodHWlb4D5doFvd3OrZHZCpFU>NIi1bJYzOjV2Nk[2PS=>
HL-60MWDBdI9xfG:|aYOgRZN{[Xl?NVnQbo5{OTBvOEFOwG0>Ml:3OIg>M3\KZolvcGmkaYTzJHRIUFFvaX7keYNm\CClZXzsJIFxd3C2b4Ppd:KhMYKyNlUzOzJ{OR?=
BCCMWHBdI9xfG:|aYOgRZN{[Xl?NWXYdnBUPTEQvF2=NHz2fVAycA>?M3:wOIlvcGmkaYTzJGRCXFNvbXXkbYF1\WRiZ4Lve5RpKGmwaHnibZRqd25?M1\Jb|IzPTF7NEO2
RAW 264.7MnPRRZBweHSxc3nzJGF{e2G7NWDIVoNUPTBxMUCw{txONWDJT4pwOWh?MXnjc45k\W62cnH0bY9vNWSncHXu[IVvfCCrbnjpZol1eyCGT16tbY5lfWOnZDDyVm5CKGOuZXH2ZYdmMlfnNlI1QTF2Mk[=
K562M37wW2Fxd3C2b4Ppd{BCe3OjeR?=Mon0NlXPxE1?NGm2UpAzcA>?MUHwdoV3\W62czDhdI9xfG:|aYOgbY5lfWOnZDDifUBkdy22cnXheI1mdnRid3n0bEBidXW{ZX7zbY4hTyCjbnSgWHJCUUx?MlLENlI1QDN5N{e=
SGC-7901NFTQNY5CeG:ydH;zbZMhSXO|YYm=MYOyNEDPxE4EoB?=MXyybC=>MlTBZZR1\W63YYTld{BJOk9{wrDvdkBVVkZizsGtbY5lfWOnZDDj[YxtKGGyb4D0c5NqeyCjczD3[YxtKGG|IHPhd5Bie2VvMzDhZ5Rqfmm2edMgMXiyNlQ4OTV6OR?=
PC3NXPP[W17SXCxcITvd4l{KEG|c3H5M{XYN|Ex|ryPNGjUWI01cA>?NXP3Z2w2[2:3boTldpMh\myjdn;jc5hq\C2rbnT1Z4VlKGOjc4Dhd4UuemWuYYTl[EBieG:ydH;zbZM>M{jZXVIzPDdzOUe0
SMMC-7721MYHBdI9xfG:|aYOgRZN{[Xl?NY\XXmZ5PTEQvF2=NYjOVmEzPDiqNV6ydpQ6[XS2ZX71ZZRmeyCxVGKtbY5lfWOnZDDhdI9xfG:|aYO=M2TKUFIzPDZ3OEOz
HeLa MmnDRZBweHSxc3nzJGF{e2G7MYW1NOKh|ryPMljGOE85cA>?NIHLXXJqdmirYnn0d{BUXFNvaX7keYNm\CCuYYTlMZBp[XOnIHHwc5B1d3SrYzDleoVvfHN?NVS5So1ROjJ2NkC1NFQ>
HeLa NXH0RXBNSXCxcITvd4l{KEG|c3H5M4Kwd|UxyqEQvF2=Mon1NYg>MkLxd5VxeHKnc4Pld{B1cGViRmLBVE1qdmS3Y3XkJIFk[3WvdXzheIlwdiCxZjDhco5mgGmwIG[gdI9{cXSrdnWgZ4VtdHN?MWqyNlQ1QTR2MB?=
T47D MWDBdI9xfG:|aYOgRZN{[Xl?M4jwNFExOM7:TR?=NVTCWmt7OWh?MnqwZoxw[2u|IITo[UBo\W6ncnH0bY9vKG:oIFWtZ4FlN0OWRkKgZpkhW1SVMmHnNlI1ODFzNki=
HeLa NVKzSm57SXCxcITvd4l{KEG|c3H5NX3Y[YRyOzEQvF2=NIHsdWw1cA>?M1f4cuKhcW6lcnXhd4V{KHSqZTDn[Y5memGuIHPlcIwhfmmjYnnsbZR6KDR6IHigZYZ1\XJicHjveI9lgW6jbXnjJJRz\WG2bXXueOKhMn:xNlI{QTR{NEi=
HCCMUHBdI9xfG:|aYOgRZN{[Xl?NInae4QzOCEQvF5CpC=>M1rrSlJpMU\heJRmdnWjdHXzJJRp\SCGSFGgbY5lfWOnZDDhZ5RqfmG2aX;uJI9nKFCDUmC=NWe1eVZOOjJ|NEK3N|I>
mESCsMXnBdI9xfG:|aYOgRZN{[Xl?MmrHNk42|ryPMUeybC=>NH:0TnFqdmirYnn0d{B1cGViTnHGMY1m\GmjdHXkJINie3Cjc3WgZYN1cX[jdHnvci=>NFjnS3ozOjJ6NUK3OC=>
EMT-6 M3vC[GN6fG:2b4jpZ4l1gSCDc4PhfS=>NXH3TWE2OTByzszNNV22cIF2OWh?MX7wZZJ1cWGubImgZoxw[2unZDDj[YxtKGSnYYToJIlv\HWlZXSgZpkhe2m{YX3ld4lv\Q>?NYHYNWx3OjJ{NUG5NlE>
MCF7MkjpRZBweHSxc3nzJGF{e2G7MmOyOVDDqM7:TR?=MYWxbC=>Ml;YbY5pcWKrdIOgVGEh[WO2aY\heIVlKGOjc4Dhd4UuOyxiLUmsJIFv\CCyb3z5LGFFWC2{aXLvd4UqKHCxbInt[ZJie2V?MY[yNlIzOzN2NR?=
K562NIXtSIdCeG:ydH;zbZMhSXO|YYm=NHGwcY0zOCEQvF5CpC=>M1vBelQ5cA>?MoTVSG1UVw>?MlWzZoxw[2u|IHzhdIF1cW6rYj3pcoR2[2WmIHnubIljcXSrb36gc4YhfmmjYnnsbZR6KGGwZDDhdI9xfG:|aYOgbY5lfWO2aX;uM2X2clIzOjF4MUW4
Molt4-hygNYnPeZlESXCxcITvd4l{KEG|c3H5M4eycVEx|ryPM{W5eVAvPWh?MnrQZoxw[2u|IH\hdo5me2:uLXnu[JVk\WRiY3HzdIF{\S1|LXzpb4Uh[WO2aY\peJk>M3:wUlI3Ojd3OEGx
HeLaNGK3SnZCeG:ydH;zbZMhSXO|YYm=MnLaNVDPxE1?M3XjfFAvPWh?M4jKZYlvcGmkaYTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KHSqZTDjc41jcW6nZDD0doVifG2nboSge4l1cCCpb33pd4lvKE5iYX7kJHRTSUmOMUKyNlE4QTZ4MR?=
Jurkat TNH7tTplCeG:ydH;zbZMhSXO|YYm=NH\zcoE{OM7:TR?=MmXzNE42cA>?M4fGTmROW09?NVH2OmhS[myxY3vzJJRp\SC8aYLhcU1qdmS3Y3XkJIFxd3C2b4Ppdy=>MlflNlIyPTl6OUi=
NeutrophilMUjBdI9xfG:|aYOgRZN{[Xl?MmPzNlDPxE1?M3rlflAvPWh?NHzaWpJifHSnboXheIV{KHSqZTDwdo8u[XCxcITveIlkKGWoZnXjeEBw\iCPYWKxNX7oWYh{OjZzOU[4OFQ>
HCT116M161WGFxd3C2b4Ppd{BCe3OjeR?=MlewOVDDqM7:TR?=NF3MO4gzcA>?MnXidoV3\XK|ZYOgd5lv\XKpaYP0bYMh[XCxcITvd4l{KGWoZnXjeJMhd2ZiY3Xs[YNwgGmkIHHu[EBPWENvMUdCpC=>MX6yNlE2QTd3Mh?=
MDA-MB-231 M1zGemFxd3C2b4Ppd{BCe3OjeR?=MkGxNk42NTdwNd88US=>NUPQW4p1Omh?NF30TJFqdmirYnn0d{B1cGViY3XscEBl\WG2aDDv[kBOTEFvTVKtNlMyKGOnbHzzJIlv\HWlZXSgZpkhW0SWIHnuJIEh[2:wY3XueJJifGmxbjDk[ZBmdmSnboSgcYFvdmW{MoDQNlIyOTV3Mk[=
LNCaPMV7BdI9xfG:|aYOgRZN{[Xl?NGjQfpc1OM7:TR?=M1P6RVJpMYXpcohq[mm2czDieZRmcW5iaX7keYNm\CClZXzsJIFxd3C2b4Ppdy=>NELLOWszOjFzNEe2OC=>
MB231MoLyRZBweHSxc3nzJGF{e2G7MVqxNFDPxE1?MVOxbC=>MnLaSG1UVw>?MofLZYJzd2ejdHXzJJRp\SCrbnT1Z5Rqd25ib3[gZ4VtdCCmZXH0bEBjgSCZRVWxJIlvcGmkaYTpc44tKFSUQVnMJJRz\WG2bXXueEwh[W6mIITo[UBkd22kaX7heIlwdg>?MnXtNlIyOTJ7NEC=
HCC38 NUHjVo9qSXCxcITvd4l{KEG|c3H5MVGxNFDPxE1?NF7hfIcycA>?MoD3SG1UVw>?MY\hZpJw\2G2ZYOgeIhmKGmwZIXjeIlwdiCxZjDj[YxtKGSnYYToJIJ6KFeHRUGgbY5pcWKrdHnvckwhXFKDSVygeJJm[XSvZX70MEBidmRidHjlJINwdWKrbnH0bY9vNGPFelYzOjFzMkm0NC=>
MDA-MB231MWrBdI9xfG:|aYOgRZN{[Xl?M4fMblUxyqEQvF2=NULTfJNZOjSqMYXEUXNQMXzhZpJw\2G2ZYOgZ5Vz[3WvaX6tbY5lfWOnZDDj[YxtKGSnYYToNFS3VVgzOjFyMUOzOS=>
LNCaPMUDBdI9xfG:|aYOgRZN{[Xl?NUTSOlR{PTEEoN88US=>MYSyOIg>NIfPdWZFVVORNH;6bI9i[nKxZ3H0[ZMh[3W{Y4XtbY4ucW6mdXPl[EBk\WyuIHTlZZRpMnrpNlIyODF|M{W=
HCT116NETydWJCeG:ydH;zbZMhSXO|YYm=NIjMeo02OMLizszNNIfpVGozPGh?NYXPRZFoTE2VTx?=NILwSVVi[nKxZ3H0[ZMh[3W{Y4XtbY4ucW6mdXPl[EBk\WyuIHTlZZRpNW\xWY1VOjJzMEGzN|U>
Ishikawa MlHRRZBweHSxc3nzJGF{e2G7NX7aRYRqOjYQvF2=MmOxNlRpMULy[YR2[2W|IHPlcIwh\GWjdHigZY5lKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IFLh[kBCOQ>?NVLKVlNyOjJyOEi5NVg>
 YD-8 MV3BdI9xfG:|aYOgRZN{[Xl?MoOyNVAx|ryPNYHwOZR2OWh?MUTpcohq[mm2czDQUGVQNWmwZIXj[YQh[XCxcITvd4l{MmLlNlIxQDZzOEO=
eosinophil NW[1THpwSXCxcITvd4l{KEG|c3H5MnHHPVDPxE1?Mlr0NlRpM2jQOolvcGmkaYTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KE[jczDhcpRq[m:medMgMVGyNlA4QTN|NB?=
L929NVjtT3RDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NFrZZXYzNjYQvF2=MoHPNlRpNXLvWpRYcW6qaXLpeJMhfGinIHX4dJJme3Orb36gc4YheC2yM{igZY5lKE6ILd86RkB1dyCjdXft[Y51KFSQRt8xMYlv\HWlZXSgcoVkem:ydH;zbZMh[W6mIHH1eI9xcGGpeR?=NHTXcpozOjB{N{C5Oy=>
YD-8 NGrkSJpCeG:ydH;zbZMhSXO|YYm=MXKxNFDPxE1?M1zOOFFpNEnKcXhjdG:la4OgeIhmKEeVLVjDcE1qdmS3Y3XkJIFxd3C2b4Ppdy=>NF63bIYzOjB{MEC3PC=>
HBxNXX3S|J7SXCxcITvd4l{KEG|c3H5Ml;2NlXPxE1?M1WxXVQ5cA>?NYnwTXR2TE2VTx?=M1fZVJJm\HWlZXSgZ4VtdCCmZXH0bEBqdmS3Y3XkJIJ6KDNvTVG=M{TuOVIzODJyMEe4
U937 MVrBdI9xfG:|aYOgRZN{[Xl?NX7zS4J3PTEEoN88US=>NF;yTJUycA>?MVPpcohq[mm2czDISk1qdmS3Y3XkJIFxd3C2b4Ppd:KhM2nKc|IyQTl6N{Ox
HL60NGq1TYlCeG:ydH;zbZMhSXO|YYm=MVO0NO69VQ>?MVu0NI1qdg>?MYfEUXNQMXvicI9kc3NiQl7ER{Bkd22yb4Xu[JMhcW6mdXPlJIV5eG:|dYLlJI9nKHCqb4PwbIF1cWS7bIPldolv\SCjbnSgSG5CKG[{YXft[Y51[XSrb36=M371S|IyQTh|Mkm2
M-14MkK5RZBweHSxc3nzJGF{e2G7MVqyOe69VQ>?MYiwMlVpMn:2xsBqdmirYnn0d{Bjd3SqIITo[UBkenWmZTDlfJRz[WO2LTDhcoQh[2:vcH;1coQucW6mdXPl[EBieG:ydH;zbZM>NGOydJkzOTl3NEm1PS=>
SK-BR-3M{SzOWFxd3C2b4Ppd{BCe3OjeR?=M4T1XlUxyqEQvF2=MYeybC=>NEHpWGtjdG:la4OgZZBweHSxdHnjJGRPSSCocnHncYVvfGG2aX;uJIlv\HWlZXSgZpkh[2:vYnnuZZRqd25ib3[gV20uOTZ2IHHu[EBz[WSrYYTpc44>M3XBUVIyQTBzM{i2
MDA-MB-468NYPIVYlvSXCxcITvd4l{KEG|c3H5Mlf0OVDDqM7:TR?=MYKybC=>NYe0V4lp[myxY3vzJIFxd3C2b4TpZ{BFVkFiZoLh[41mdnSjdHnvckBqdmS3Y3XkJIJ6KGOxbXLpcoF1cW:wIH;mJHNONTF4NDDhcoQhemGmaXH0bY9vMXyyNVkxOTN6Nh?=

... Click to View More Cell Line Experimental Data

In vivo
Features A key compound for apoptosis studies.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDog
Weight (kg)0.020.151.80.40.0810
Body Surface Area (m2)0.0070.0250.150.050.020.5
Km factor36128520
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)
1

References

[1] Slee EA, et al. Biochem J, 1996, 315 (Pt 1), 21-24.

[2] Shimizu T, et al. Leukemia, 1997, 11(8), 1238-1244.

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Chemical Information

Download Z-VAD-FMK SDF
Molecular Weight (MW) 467.49
Formula

C22H30FN3O7

CAS No. 187389-52-2
Storage 3 years -20℃powder
2 years -80℃in solvent
Synonyms N/A
Solubility (25°C) * In vitro DMSO 93 mg/mL (198 mM)
Water <1 mg/mL (<1 mM)
Ethanol 24 mg/mL warming (51 mM)
DMF 84 mg/mL
* Z-VAD-FMK will racemize in DMSO, water and ethanol.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name L-Alaninamide, N-[(phenylmethoxy)carbonyl]-L-valyl-N-[(1S)-3-fluoro-1-(2-methoxy-2-oxoethyl)-2-oxopropyl]-

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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