Z-VAD-FMK

Catalog No.S7023

Z-VAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.

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Z-VAD-FMK Chemical Structure

Z-VAD-FMK Chemical Structure
Molecular Weight: 467.49

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Product Information

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Product Description

Biological Activity

Description Z-VAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.
Targets Pan-caspase [1]
(THP.1, Jurkat T-cells)
In vitro Z-VAD-FMK (10 mM) inhibits apoptosis in THP.1 cells. Z-VAD-FMK (10 μM) inhibits activation of PARP protease activity in control THP.1 cell lysates. Z-VAD-FMK (10 mM) inhibits the processing of CPP32 in intact THP.1 and Jurkat cells. [1] Z-VAD-FMK (50 μM) cotreatment abolishes the apoptotic morphology of camptothecin-treated HL60 cells. Z-VAD-FMK (50 μM) blocks camptothecin-induced DNA fragmentation in HL60 cells. [2] Z-VAD-FMK (50 μM) inhibits cell death following dSMN dsRNA-induced apoptosis in S2 cells. Z-VAD-FMK (50 μM) increases the percentage of transfected cells surviving from 26% to 63% in S2 cells. [3] Z-VAD-FMK (> 100 μM) enhances TNFα-induced neutrophil apoptosis, lower concentrations (1-30 μM) completely blocks TNFα-stimulated apoptosis in human neutrophils. [4] Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes. Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes detected with the TUNEL assay. [5]
Cell Data
Cell LinesAssay TypeConcentrationIncubation TimeFormulationActivity DescriptionPMID
GC1aNU\EVGNQSXCxcITvd4l{KEG|c3H5MoDyOVDPxE1?NYC5[VNVOjSqM3jOeIlv\GmlYYTld{BiKHC{b4TlZ5RqfmViZX\m[YN1KGGpYXnud5Qh\XSxcH;zbYRmMnPVNlYyPjlyN{W=
HGL5NVPZT2U2SXCxcITvd4l{KEG|c3H5NVH0b|hFPTEQvF2=NXS3XpZlOjSqM{\2O4lv\GmlYYTld{BiKHC{b4TlZ5RqfmViZX\m[YN1KGGpYXnud5Qh\XSxcH;zbYRmNV7ENoNbOjZzNkmwO|U>
HepG2MofFRZBweHSxc3nzJGF{e2G7NIPRVJMzOM7:TR?=NWDGcJI4OWh?MYfheJRmdnWjdHXkJJRp\SCjcH;weI91cWNiaX7keYN1cW:wIH;mJGlKUS1zMNMgNEj3T3kzPjF4NEe5OS=>
BEL-7402Mn\6RZBweHSxc3nzJGF{e2G7M3yxVlIx|ryPM4XPXFFpM4rHN4F1fGWwdXH0[YQhfGinIHHwc5B1d3SrYzDpcoR2[3Srb36gc4YhUUmLLUGwxsA>MnjFNlYyPjR5OUW=
CEFNVz2SXJKU2mwYYPlJGF{e2G7MlG0NEw{Oyx4NzyxNFDPxE1?MX2xOY1qdg>?NHHQWHBld3ewLYLl[5Vt[XSnczDQVkBmdnq7bXWgZYN1cX[rdImgeI8hPDExvJWgZZQhOTByzszNM2nQcVI3OTB{M{O5
SP2/0M4mx[WFxd3C2b4Ppd{BCe3OjeR?=MVqxNFDPxE1?NGL3OJcycA>?MnXwSG1UVw>?NWKyN2FV[myxY3vzJJRp\SCjcH;weI9{cXNib3[gV3AzNzBiY3XscJM>NFnhW|AzPjB5NEezNi=>
HUVEC-2cM37ifGFxd3C2b4Ppd{BCe3OjeR?=NE\RVng2OM7:TR?=MV[2bC=>MVvk[YNz\WG|ZXSgeIhmKG:6LVzEUE1qdmS3Y3XkJIF2fG:yaHHnfS=>MWWyOlAzOTd{OR?=
U1M1f3OmFxd3C2b4Ppd{BCe3OjeR?=M2C2RlAuOTByzszNM2DFblJpNWXGSpZHemWmdXPld{BlenWpLXnu[JVk\WRiYYDvdJRwe2m|IHHu[EB{fWK|ZYH1[Y51KEiLVj2xJJJmeGyrY3H0bY9vKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVzM2\WelI2QThyOUSy
ACH-2Mn;4RZBweHSxc3nzJGF{e2G7NVLXcG1jOC1{MEFOwG0>NGjGZ48zcA>?MYTy[YR2[2W|IHTyeYcucW6mdXPl[EBieG:ydH;zbZMh[W6mIIP1ZpNmeXWnboSgTGlXNTFicnXwcIlk[XSrb36gbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK=NIfEUW0zPTl6MEm0Ni=>
U1NXG5e2JMU2mwYYPlJGF{e2G7NIXkdHMyODEQvF2=NEXlc|MzcA>?M1zwOolvcGmkaYTzJINie3Cjc3WtNy=>MYOyOVk5ODl2Mh?=
A549/V16MWrBdI9xfG:|aYOgRZN{[Xl?MXe1NO69VQ>?NHu3cm4ycA>?Ml;JdoVxemW|c3XzJHRIN1SPLXnu[JVk\WRiYYDvdJRwe2m|MViyOVk1PjB|Mx?=
SGNNHG4VolCeG:ydH;zbZMhSXO|YYm=MWWyNI1ONE\FS2w1QGh?NXHwZmE{cGG|IH7vJIlv\my3ZX7j[UBwdiCDSV[sJINidHCjaX6g[ZhxemW|c3nvckBweiClZXzsJIFxd3C2b4Ppdy=>Mlz3NlU5PzR4M{O=
HCT116 M{LDb2Fxd3C2b4Ppd{BCe3OjeR?=M1fBWFUx|ryPMn\CNog>MYDpcohq[mm2czD0bIUh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IFTEWnA>NGDKOpozPTh4OEixPC=>
DTK-SMENETuUoJCeG:ydH;zbZMhSXO|YYm=MUC1NO69VQ>?NU\YbJJ6Omh?MYnEUXNQMWHwZZJ1cWGubImgbY5pcWKrdIOgeIhmKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IHL1dIl3[WOjaX7lNVPhfGRQOjV6NEO4PVc>
HL-60NIX2PI5CeG:ydH;zbZMhSXO|YYm=MWG1NO69VQ>?MoHtOFhpNWH1OGhvemWmdXPld{Bk\WyuIHTlZZRpKGOjdYPl[EBjgSCVS1mtTWkhfHKnYYTt[Y51yqB?MWKyOVgzPDB2Mx?=
U937M2riR2Fxd3C2b4Ppd{BCe3OjeR?=NGiy[VM2OM7:TR?=NYrrboVsPDiqMYfy[YR2[2W|IHPlcIwh\GWjdHigZ4F2e2WmIHL5JHNMUS2LSTD0doVifG2nboVCpC=>Ml;SNlU5OjRyNEO=
A549MVfBdI9xfG:|aYOgRZN{[Xl?Mk\MNlAh|ryPNFHrcHczPGh?M4HIWJJm\HWlZYOgZ4VtdCCmZXH0bEBk[XW|ZXSgZpkhUE[FUDD0doVifG2nboS=MnuzNlU4QTRzNEm=
HeLaM2XtPWFxd3C2b4Ppd{BCe3OjeR?=MVKxNO69VQ>?M3i0c|Q5cA>?NIS2W|Rl\WOuaX7ld{B1cGVicnH0[UBw\iCjcH;weI9{cXNiZILhcYF1cWOjbHz5NIK1d44zPTd5MkW0OS=>
SGC-7901MmXxRZBweHSxc3nzJGF{e2G7NYHRSoVWOjEEoN88US=>NFW5UYoycA>?MUXpcohq[mm2czD0bIUh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IH;4ZYxqeGyjdHnuNH3sTmUzPTd4N{C3Oi=>
MCF-7NYC0O4xNSXCxcITvd4l{KEG|c3H5M2\rWVEx|ryPNV[0[nF6Omh?NYryWZpwcW6qaXLpeJMhfGinIHPlcIwh\GWjdHigbY5lfWOnZDDifUA{Sg>?NXzQW5hTOjV5MkKxNVQ>
A549NW[z[ZlNSXCxcITvd4l{KEG|c3H5MVOxNEDPxE1?MXqyOIg>NYqx[XlrTE2VTx?=NXrxUI15eHKxbX;0[ZMhUEKFLYTy[YF1\WRiQUW0PUBk\WyuIIP1dpZqfmGuID[gZZR1\W63YYTld{B1cGViY3zlZZZm\CCSQWLQJIV5eHKnc4Ppc44>MoLvNlU3QDN3Nki=
U87MnXmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NVPVOWtUPTEQvF2=NYXZdpdxOWh?NXXKTpRtemWlb4\ldpMh[2WubDDndo94fGhiZoLvcUBVVVpidILlZZRu\W62MmLtNlU3QDF4Nki=
Neocortical NeuronNUTPS2NiVmW3cn;0c5hq[2m2eTDBd5NigQ>?MkL6NVAx|ryPNH7Yb3IycA>?Mmj3ZY51[Wexbnn6[ZMhUG:rYX3p[IUhSS2LbnT1Z4VlKE6ndYLveI95cWOrdIm=MoPoNlU3PzVyMEG=
Primary human placental cytotrophoblastsMnn4RZBweHSxc3nzJGF{e2G7NX;kTW5oOzEQvF2=MXuyOIg>Ml;wSG1UVw>?Mn;DdoV3\XK|ZYOgeIhmKGmwaHnibZRqd25ib3[gNVHPui2KU1SyJIJ6KHS{aXPsc5NidsLiMY[yOVY1OjV7Mh?=
MMMlraRZBweHSxc3nzJGF{e2G7NVTiT3A5PTEQvF2=MXeyNI1qdg>?MnPHdIFzfGy7IHnubIljcXS|IGPIT{1qdmS3Y3XkJEBk\WyuIHTlZZRpNXLnPWZROjV3M{CwPVg>
DLD1MlfZRZBweHSxc3nzJGF{e2G7M1;ZTlIxyqEQvF2=MXyxbC=>NHK5[FZx[XK2bImgdoV3\XK|ZYOgZ4VtdCCjcH;weI9{cXNiY3H1d4VlKGK7IGfTVFE>NXr6UHNoOjV3MkSyOFY>
MDA-MB-231NXrSUnhPS3m2b4TvfIlkcXS7IFHzd4F6NV\WdlNnOTEQvF2=NV3tPXNtOWh?MWjheYdu\W62czDj[YxtKGSnYYToJIFnfGW{IFPIWWEhfHKnYYTt[Y51MlG5NlU2OjF3MEG=
MKN28NHjPcFFCeG:ydH;zbZMhSXO|YYm=NETRfZYyOM7:TR?=MXizNI1qdg>?MlvoSG1UVw>?MXXpcohq[mm2czDUUmYu|rFicHz1d{BEUFhvaX7keYNm\CCjcH;weI9{cXN?NIjDe3YzPTVzM{m2NC=>
HL-60NWfDWIVsS3m2b4TvfIlkcXS7IFHzd4F6NUftUXJPOTByzszNNVvCS2kyOWh?Mn[2SG1UVw>?MonKxsBqdXC{b4\ld{B3cWGkaXzpeJkhd2ZiVFPORZMufHKnYYTl[EBk\Wyucx?=M4O5bVI2PTB{OUOy
AGSMlr1RZBweHSxc3nzJGF{e2G7M1i3dFEx|ryPMorwNYg>NH3NOYdxemW4ZX70d{BkfXKldX3pck1qdmS3Y3XkJINt\WG4YXflJI9nKGOjc4Dhd4UuOyxiLUisJIFv\CBvOTDwdo91\Wmwcx?=M{GwVlI2PDl{MkG0
A549 MkK2RZBweHSxc3nzJGF{e2G7NYfxVVVCOTEQvF2=NHL0RpAzPGh?MVLicI9kc3NiRF3BV{1qdmS3Y3XkJINt\WG4YXflJI9nKGOjc4Dhd4UuOyCjbnSgVGFTWCCjbnSgZZBweHSxdHnjJINmdGxiZHXheIg>M{LhUFI2PDN2OUi5
INS-1NEHsOZZCeG:ydH;zbZMhSXO|YYm=NULIfXpmPTEQvF2=NGO2ZnI3cA>?M3zFR2ROW09?NGrLO|Nl\WO{ZXHz[ZMhfGinIIDyc5BwenSrb36gc4Yh\WG{bImgZZBweHSxdHnjJINmdGy|wrC=NFzve4czPTR|MEi5Oy=>
Primary hepatocytes NXX5VnRtSXCxcITvd4l{KEG|c3H5MYq1NO69VQ>?NIjmclQyQGh?NELO[VRqdmirYnn0d{BieG:ydH;zbZMhd2ZiaHXwZZRw[3m2ZYOgbY5lfWOnZDDifUBC[3RiRDDhcoQhXE6ILd8xMYmyOVQxPzV|OB?=
L929-A NFrPPXZEgXSxdH;4bYNqfHliQYPzZZk>NVvhN5BZOjEEoN88US=>NWfLUZBuOjSqMkfMbY5pcWKrdIOgWG5H|rFvaX7keYNm\CClZXzsJIRm[XSqM3zUeVI2Ozl6NUSw
L929-NM2S5OWN6fG:2b4jpZ4l1gSCDc4PhfS=>NV7EfHY2OjEEoN88US=>NUHFV4ttOjSqMnP0[Y5p[W6lZYOg[IVifGhidnnhJIF2fG:lcnnu[UBVVkcQsTDwdo9lfWO2aX;uM2LOZlI2Ozl6NUSw
Cytotoxicity AssayM2LDcWNmdGxiVnnhZoltcXS7IFHzd4F6NV3BSW5iOjEQvF2=NE\aWVM1QGh?NXzofnBDeHKndnXueEBOUE2GLXnu[JVk\WRiY3XscEBl\WG2aB?=NUHLXnQyOjV|OUKxNVY>
COS7MYPLbY5ie2ViQYPzZZk>M3LOXVEx|ryPNV\KTHhUOjSqMmW3bY5kemWjc3XzJINie3Cjc3WgN{84KGGldHn2bZRq\XN?NYe3NXpxOjV|OESwNlU>
COS7M1zU[WFxd3C2b4Ppd{BCe3OjeR?=NUXxVHM4OTEQvF2=M13GN|Q5cA>?M1nofpBienSrYXzsfUBxemW4ZX70[YQhTkNzMEGtbY5lfWOnZDDj[YxtKGSnYYToMW[yOVM5PDB{NR?=
A375NUTzUWxMSXCxcITvd4l{KEG|c3H5M{iyXFMx|ryPMmi3Nog>MXrwdoV3\W62czD0bIUh\HK3Zz3pcoR2[2WmIGDBVnAh[2ynYY\h[4U>NIjIXnYzPTN5NkGxOS=>
A549MVTBdI9xfG:|aYOgRZN{[Xl?MmLnOVDPxE1?M3e4fFI1cA>?MkfNdoV3\XK|ZYOgdoljd3OxbXWgZolw\2WwZYPpd{BidmRiYYDvdJRwe2m|IHPheZNm\CCkeTCgR4hqcm:wZ3Thci=>M{XGRVI2OzR7N{ix
A549MXXBdI9xfG:|aYOgRZN{[Xl?MkjpNk42NTJ3zszNMkXrNYg>MX\k[YNz\WG|ZYOgeIhmKHCxcIXsZZRqd25ib3[gZZBweHSxdHnjJINmdGy|IHTldIVv\CCxbjDjc45k\W62cnH0bY9vew>?MVSyOVM1OjR{Nx?=
 NBL-W-S NV;MOYJoSXCxcITvd4l{KEG|c3H5MWO1NO69VQ>?NHviVXcycA>?NGC0O4pnfWyueTDy[ZNkfWW|IHPlcIwhfmmjYnnsbZR6KGGodHXyJGdCVlRvNkGgeJJm[XSvZX70M{D6VFI2OzJ|MkKy
Caki-1 MYLBdI9xfG:|aYOgRZN{[Xl?NH;hR201OM7:TR?=MYi0PIg>MlzQdoVlfWOnczD0bIUhdnWvYnXyJI9nKEGwbnX4bY4hXi2yb4PpeIl3\SClZXzsdy=>NHTJbWszPTJ5OUG5NS=>
769-PNF3xRYhCeG:ydH;zbZMhSXO|YYm=M4fXcVQx|ryPMlu1OFhpMnnVdoVlfWOnczD0bIUhdnWvYnXyJI9nKEGwbnX4bY4hXi2yb4PpeIl3\SClZXzsdy=>NX;MR2J6OjV{N{mxPVE>
H9c2M3\jfGFxd3C2b4Ppd{BCe3OjeR?=Mn7mOVDPxE1?MXmxbC=>M1vpeYlvcGmkaYTzJGRQYC2rbnT1Z4VlKGOjc4Dhd4UhOyCjY4TpeoF1cW:wIHL1eEBvd3RidHjlJIxwe3Nib3[gZ4VtdHN?M13leVI2Ojh|OEG5
K562NW\YNHl{SXCxcITvd4l{KEG|c3H5MUG1NO69VQ>?NGPMb2g1cA>?MVLpcohq[mm2czDKZYMuSS2rbnT1Z4VlKGOnbHygZZBweHSxc3nzMVWyOVI1OTZzOR?=
L929NF3OcJJCeG:ydH;zbZMhSXO|YYm=NVz0RmVEOTEQvF2=NYLPTpB2Pmh?Mmi4bY5lfWOnczDu[YNzd3C2b4Ppd{B4cXSqIGTOSi=>Ml\VNlUyQTV4NkC=
A549MoixRZBweHSxc3nzJGF{e2G7M1LqZlEx|ryPMlvyN4g>NX\vb2RIemWmdXPld{Bk\WyuIHHwc5B1d3OrczDjZZV{\WRiYomgVHFSNE\W[ngzPTF4MU[5PS=>
MCF-7M3TJfGFxd3C2b4Ppd{BCe3OjeR?=NFTPZVMzOM7:TR?=NYjJT|ViOmh?MYfk[YNz\WG|ZYOgT2RTNXOrUl7BMYlv\HWlZXSgZZBweHSxc3nzM1HydVI2OTh{MkSw
OSNEnYdI1CeG:ydH;zbZMhSXO|YYm=NWnMR4o3OjBxNEFOwG0>MWe3Nog>MXrpcohq[mm2czDPV{Bk\WyuII\pZYJqdGm2eTDy[YR2[3Srb36gZpkhSzZiY3XyZY1q\GV?M4HXclI2OTV{M{m5
HUVECsM1LzcGFxd3C2b4Ppd{BCe3OjeR?=MYSxNO69VQ>?M4fzc|JpM4\xNeKhcW6qaXLpeJMh[XCxcITvd4l{KGGwZDDmZYNqdGm2YYTld{BifXSxcHjh[5khcW5iRFXOWlIucW6oZXP0[YQhUFWYRVPzNEn0cFkzPTF|OEewNy=>
EA.hy926Mn75RZBweHSxc3nzJGF{e2G7M{T5VFEx|ryPMn;SNog>NHXGOGjDqGmwaHnibZR{KGGyb4D0c5NqeyCjbnSg[oFkcWyrdHH0[ZMh[XW2b4DoZYd6KGmwIFTFUnYzNWmwZnXjeIVlKEWDLnj5PVI3NH;5fFUzPTF|OEewNy=>
EbsNXj5cnkxSXCxcITvd4l{KEG|c3H5Moj5NVAuOTByzszNNFO2flYzPGh?MXxCpG1OXFNiZ3Xu[ZJifGmxbjDyZZRmKGSnY4LlZZNm\CCjczD0bIUh[2:wY3XueJJifGmxbjDv[kB7NV[DRD7mcYshcW6lcnXhd4VlNVjoZWpUOjVzM{S4NVc>
JurkatNXTzXFViSXCxcITvd4l{KEG|c3H5NGHB[JMzOM7:TR?=M{mxbVI1cA>?NXj1TmhITE2VTx?=Mn7idIFzfGmjbHz5JIlvcGmkaYSgZ4VtdCCmZXH0bEBw\iCMdYLrZZQh[2WubIOgbY5lfWOnZDDifUAyODB3OD3GOEBkd22kaX7l[EB4cXSqIG\QReKhNGLLT4kzPTF{MEeyNy=>
K562MmnZRZBweHSxc3nzJGF{e2G7Mn7rNE4yNTIQvF2=NWi0Rm1{OWh?MkfqbY5pcWKrdIOgZ4xm[X[jZ3Wgc4YhUFOSOUCgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK=NEXQSXkzPTFzOUG4PC=>
IMR-32Moj1RZBweHSxc3nzJGF{e2G7M4LiOlQxyqEQvF5CpC=>NX[5OnF4Omh?NUTIcnNY\GWlcnXhd4UhcW5iYYDvdJRwfGmlIHPlcIx{KGOxbYDhdoVlKHSxIGStNkB1d3irbh?=Mof4NlUxQDR5NUW=
RPMI 8226NX3wWpJXSXCxcITvd4l{KEG|c3H5MmmxNVAx|ryPM4C2[VFpMWnhcI1we3RiY3;tdIxmfGWueTDicI9kc3NiY3XscEBl\WG2aDDjZZV{\WRiYomgcoVkem:|dHH0bY4uOQ>?NFjZfVczPTB|N{i1NS=>
U266 M2rIOWFxd3C2b4Ppd{BCe3OjeR?=NGXQZ5oyODEQvF2=M2[5c|FpNULxbVZ{eGG{dHnhcIx6KGKub3Prd{Bk\WyuIHTlZZRpKGOjdYPl[EBjgSCwZXPyc5N1[XSrbj2xMXuyOVA{Pzh3MR?=
H929 MlrQRZBweHSxc3nzJGF{e2G7MkfTNVAx|ryPM{TDZlFpMXnwZZJ1cWGubImgZoxw[2u|IHPlcIwh\GWjdHigZ4F2e2WmIHL5JI5m[3Kxc4TheIlvNTF?NWT4cJdPOjVyM{e4OVE>
MM.1S MVrBdI9xfG:|aYOgRZN{[Xl?Mn3CNVAx|ryPM3TIWVFpMmXq[YZncWOrZX70cJkheHKndnXueJMhe2:{YX\lcoljNWmwZIXj[YQh[2WubDDk[YF1cCClb33ibY5mKHerdHigcoVkem:|dHH0bY4uOQ>?MV[yOVA{Pzh3MR?=
C6NGLlfIpCeG:ydH;zbZMhSXO|YYm=NXP1N4ZJPTEQvF2=NFLoTnA1QGh?M4T6cZBz\X[nboTzJJRp\SCub4PzJI9nKGOnbHygeoli[mmuaYT5JINifXOnZDDifUBxemWpbnXuc4xwdmV?NGi3dmIzPTBzM{S3PS=>
AGSMl:1RZBweHSxc3nzJGF{e2G7Mnu2OVDPxE1?M3LyU|I1cA>?MnjpZYJwdGm|aIOg{tIudGGyYXPoc45mNWmwZIXj[YQh[2WubDDk[YF1cCCjbnSgbY5pcWKrdHXkJIdzd3e2aB?=MnvENlUxODl4OUi=
HeLa M4LZOGFxd3C2b4Ppd{BCe3OjeR?=NXLoW3BWPDEQvF2=NXHSOlc3OjSqNYLZbnJ[cW6qaXLpeJMhfGinIHnuZ5Jm[XOnZDDhdI9xfG:|aYOgbY5lfWOnZDDifUB{cVKQRkGyNS=>M1Xac|I1QTJ6Nki1
THP-1 M2rBR2Fxd3C2b4Ppd{BCe3OjeR?=MVqxNO69VQ>?NILFPXAycA>?MUDy[YR2[2W|IHHwc5B1d3OrczDpcoR2[2WmIHL5JGFNSS2VRGVCpC=>M4nCPVI1QTJ|NkWz
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fetal rat lung fibroblasts MVHLbY5ie2ViQYPzZZk>MWS4NO69VcLiNXm0VGdXOWh?NFrCVlhqdmirYnn0d{BE[XOyYYPlMVMh[WO2aY\peJnDqA>?NIC4OHkzPDNzNEGzOS=>
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HeLa NFPtRnpCeG:ydH;zbZMhSXO|YYm=NGPybGMzOM7:TR?=MWSybC=>NXnWdpZQcW6qaXLpeJMhTE2PUDDpcoR2[2WmIHHwc5B1d3Orcx?=NGDWeGozOzh4M{m2Oi=>
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HL-60NYXWNpFMSXCxcITvd4l{KEG|c3H5MV61NOK2VQ>?NF:xRY41cA>?NIrt[|FjdG:la4OgeIhmKGOuZXH2ZYdmKG:oIHPhd5Bie2VvMzygMVktKGGwZDDQRXJRKGmwZIXj[YQh[nliNoO=NGnte44zOzhyNEewOi=>
Ec-109M{i1UWFxd3C2b4Ppd{BCe3OjeR?=NHnpSZEyOCEQvF2=NX[wNmRuPDiqM4XqWpJmeHKnc4Pld{BRTS2vZXTpZZRm\CCHYz2xNFkh[2WubDDhdI9xfG:|aYO=NFrKW|IzOzd6Mk[0NS=>
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JurkatMYfD[YxtKF[rYXLpcIl1gSCDc4PhfS=>M2WwdlExOC1{MEFOwG0>MXeyOIg>NEHSOmdqdmirYnn0d{BJ[UF2IHnu[JVk\XNiYYDvdJRwe2m|IHTldIVv\GWwdDDv[kBkd26lZX70doF1cW:wwrC=MXmyN|c{OjR6MR?=
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HM7 M1GwNmFxd3C2b4Ppd{BCe3OjeR?=NV\JSHlJOjEQvF5CpC=>M1HtTFFpMWjicI9kc3NiYYDpZ5Vt[XKnbjDBJIFk\XSjdHWtbY5lfWOnZDDjZZNx[XOnLUOgZYN1cX[jdHnvckBidmRiUFHSVEBkdGWjdnHn[S=>NVniVFF6OjN3OEO0NVI>
Hep-2 NH60[|lCeG:ydH;zbZMhSXO|YYm=NEXVV|kzOM7:TdMgM33tVlI1cA>?NXvvWHJ7TE2VTx?=MnvuZYxt\X[rYYTld{B1cGViZHXjdoVie2Vib3[gZ4VtdCC4aXHibYxqfHliaX7keYNm\CCkeTDzbYxq[mmwaX6=M4DjR|I{PThyMEOy
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C666-1Mn61RZBweHSxc3nzJGF{e2G7NHjm[Jg2OM7:TR?=M{SyPFI1cA>?M3flZ5N2eHC{ZYPz[ZMhfGinIHPs[YF3[WenIH;mJHBCWlBiYX7kJINie3Cjc3WtN{whNTduIHHu[EAuQSCrbnT1Z4VlKGK7IHLvdpRmgm:vaXKvV2FJSQ>?M3XaTlI{PDd3OUW2
Hepa1-6M4PBSmFxd3C2b4Ppd{BCe3OjeR?=MnnKOVDPxE1?M1XwZVJpNVjpfo5qcW6qaXLpeJMh[3W{Y4XtbY4h[W6mIILld5ZmemG2cn;sMYlv\HWlZXSgZZBweHSxc3nzNXrPO|h1OjN2NE[3OVM>
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Primary rat cerebral cortical neuronsNYfoW4c4SXCxcITvd4l{KEG|c3H5M4rOT|ExOM7:TR?=MlX3NYg>NV;TeZRneHKndnXueJMhS2RvaX7keYNm\CCjcH;weI9{cXNiYX7kJINmdGxiZHXheIg>M1XBUFI{PzRzM{G3
MDA-MB-231 M3vVOGFxd3C2b4Ppd{BCe3OjeR?=MlSzNlXPxE1?Mn3KNlRpNU\1dph6[WK{b3fheIV{KGO7dH;0c5hq[2m2eTDhcoQh[2ynYY\h[4Uhd2ZiY3HzdIF{\S1|IHHu[EBRSVKSIHnu[JVk\WRiYomgVoc{MmPRNlU{Ozd3NES=
NLRP3-Tet-on-MC/9 M1vUTmFxd3C2b4Ppd{BCe3OjeR?=M{DwTlExNTRyzszNMUixNog>NWXvcnlj[XS2ZX71ZZRmeyC2aHWgZ4VtdCCmZXH0bEBk[XW|ZXSgZpkhS0GSUz3hd5Nw[2mjdHXkJG5NWlB|IH31eIFvfHNiaX6geIhmKFSndD3vckB{gXO2ZX2=MXOyN|cxOzN6OR?=
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MCF-7 NIfJ[nlCeG:ydH;zbZMhSXO|YYm=M1jXO|Ix|ryPwrC=Mm\sO|JpNUe0UppDcW6qaXLpeJMh\XG3b3ytJIFv\CB2LV;IWE1u\WSrYYTl[EBieG:ydH;zbZM>MVSyN|Y4PTZ2Mx?=
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astrocytes cellNXf3PWY5SXCxcITvd4l{KEG|c3H5NEfX[G01OM7:TR?=M3f4dlZpNFvaOIVz\WS3Y3XzJIViemy7IHHwc5B1d3OrczDpcoR2[2WmIHL5JJN1[XW{b4Pwc5JqdmV?MXOyN|QyOTd5OB?=
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HeLa NYjHXW5WSXCxcITvd4l{KEG|c3H5NEDmTHYyODEQvF2=MUiybC=>NHK0dGFjdG:la4OgTnJUNTF3IFnu[JVk\XNiQYDvdJRwfGmlIFPlcIwhTGWjdHi=MUOyN|M1PDB2NR?=
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H460 M3XjXmFxd3C2b4Ppd{BCe3OjeR?=MkDrNVDPxE1?NGG5bFE3cA>?NG\zdGJjdG:la4OgZZBweHSxdHnjJINwdWKrbnH0bY9vKG:oIGTheE1UdWGlTkegZY5lKHKjZHnheIlwdg>?NGPLXZozOzN|OEW2PC=>
HeLa M4K5fWFxd3C2b4Ppd{BCe3OjeR?=MVG1NO69VQ>?NF;CZXEyNjWqM3zoPYFjem:pYYTld:KhS2iuYX35[IliNWmwZIXj[YQh[XCxcITvd4l{NYDYPFlNOjN|MEO4NFQ>
SK-HEP1MX;BdI9xfG:|aYOgRZN{[Xl?MVmxNFDPxE1?M3f6dVFpMWrpcohq[mm2czDDdnQyKGGldHn2ZZRm\CClYYPwZZNmNTNuID23MEAuQCxiLUmsJIFv\CCyb3z5LGFFWC2{aXLvd4UqKHCxbInt[ZJie2V?NWLIU45jOjN|MEK2OVA>
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HepG2NFq1elBCeG:ydH;zbZMhSXO|YYm=NFXSSFgzOM7:TR?=M1r2dVMxdWmwMYfpcohq[mm2czD0bIUh\W6qYX7j[YQh[2WubDDk[YF1cCCkeTDjc41jcW6nZDD0doVifG2nboSgc4Yh[XCrZ3XubY4h[W6mIGTSRWlNMWCyN|IzPDJ|OR?=
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HSC-2MnnORZBweHSxc3nzJGF{e2G7MYqyOU82OM7:TR?=MWWxbC=>NUf6cI9ocW6qaXLpeJMhW05vM{itbY5lfWOnZDDjfZRwfG:6aXPpeJk>NHrvZlkzOzF3NUK0PC=>
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Bel-7402 Ml7QR4F{eGG|ZTDBZ5RqfmG2aX;uJGF{e2G7MmTwOVDPxE1?M{HsVFJpMV;pcohq[mm2czDQR2UucW6mdXPl[EBidm:ra3nzNEn6[WMzOzByOEe0Ni=>
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Bel-7402 NVXGV|hxSXCxcITvd4l{KEG|c3H5M33vfVUx|ryPNHnaNpEzcA>?M{jpUolvcGmkaYTzJHBETS2rbnT1Z4VlKGGwb3nrbZM>MXKyN|AxQDd2Mh?=
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RCC MXTD[YxtKF[rYXLpcIl1gSCDc4PhfS=>MXWxNFDPxE1?M{jhe|I1cA>?NHPW[|Zz\WOxdnXy[ZMhfGinII\pZYJqdGm2eTDv[kBk\WyuczDlfJBwe2WmIITvJFE2\C2SR1qyNEHOVFgzOjl7MUS5OC=>
NB2a/d1M1fjbGFxd3C2b4Ppd{BCe3OjeR?=NFHBU5YyODEQvF2=NVG5V29DPzKqNXOwNXdv[XS2ZX71ZZRmeyC|dHH1do9{eG:{aX7lMYlv\HWlZXSgZ4F{eGG|ZTDhZ5Rqfmm2eTygVGFTWCxiYX7kJJRifSClbHXheoFo\Q>?NELhNI4zOjl6OEW0NS=>
T cellNGfIeW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=Mmm0NlUuOTByzszNNH;OeoIzPGh?NUe2cXN[\G:|ZT3k[ZBmdmSnboTsfUBqdmirYnn0[YQhXCClZXzsJJBzd2yrZnXyZZRqd25ibXXkbYF1\WRidHjyc5VocCC2aHWgZ48ue3SrbYXsZZRqd25id3n0bEBidnSrLVPEN{BidmRiYX70bU1ETDJ6NGDxdoMzOjl6MkWzPC=>
K562NGTmU|VCeG:ydH;zbZMhSXO|YYm=NIewc3UyODEQvF2=NYfST2g2OWh?MYLicI9kc3NiQXLuc4Jifmm|Y4XtxsBHNWmwZIXj[YQh[XCxcITvd4l{NI\n[okzOjl5MkO3Ni=>
Jurkat MXvBdI9xfG:|aYOgRZN{[Xl?NHzwU2k1OM7:TR?=NIf1cJoycA>?Mkm1ZYJwdGm|aHXzJGZie0xvaX7keYNm\CClYYPwZZNmKGGldHn2ZZRqd25iYX7kJINmdGxiZHXheIg>NGGyPGYzOjl2MkezPC=>
BGC-823NH7G[5JCeG:ydH;zbZMhSXO|YYm=NHi1RWsyODEQvF2=MlfQNYg>NHLiUlBx[XK2aXHscJkhemW|Y4Xld{Bk\WyuczDh[4FqdnO2IHThcYFo\SCxZjDkZYllgmWrbh?=MnvRNlI6OjZ3NEW=
Hep3BNEDzPGJCeG:ydH;zbZMhSXO|YYm=NXT3b5RWPTEQvF2=M1HlN|FpMl7jZoxw[2u|IHHwc5B1d3OrczDpcoR2[2WmIHL5JGhGT0O|Mlm2NlI6OjNzNUS=
LLC-PK1M3XUUWFxd3C2b4Ppd{BCe3OjeR?=NWK5O4N6OjEQvF2=MlnwNYg>MVLwdoV3\W62czDjbZNxdGG2aX6tbY5lfWOnZDDk[Ydz[WSjdHnvckBw\iCDdHe1MEBj\WOuaX6tNUwh[W6mIFH0[|EzKHC{b4TlbY5{MlLINlI5QTZyM{e=
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MCF-7, MDA-MB-468, Caco-2NUnWXo1mT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M36zR|Ux|ryPNIDD[o01QGh?MYjpcohq[mm2czD0bIUh[2WubDDndo94fGhiaX7obYJqfGmxbjDpcoR2[2WmIHL5JJNieG:waX6=MWOyNlgxODl4OB?=
A2750 NVLuXZZ{SXCxcITvd4l{KEG|c3H5NHe4do0zOM7:TR?=NXSzbVQ6Omh?MnXxSG1UVw>?NY\GVYJS[myxY3vzJINie3Cjc3WgZ4xm[X[jZ3Wg[JVzcW6pIHjlcIVv[WyrbjD0doVifG2nboSgZY5lKHKnZIXj[ZMh[XW2b4DoZYdq[yClZXzsJIRm[XSqNEfhd4MzOjd6NEO2Ny=>
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HT1080NHXqeFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M{\4S|Ux|ryPMkjlO4Q>NXTs[pJTcW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJIlvcGmkaYTpc44h[2G3c3XkJIJ6KGOxbXLpcoVlKHS{ZXH0cYVvfCCxZjDER2Eh[W6mIF;NVC=>MkfjNlI4PDB7OES=
A549 MnH3RZBweHSxc3nzJGF{e2G7MmTPOVDPxE1?NHHEdpkzcA>?NFjUdIZx[XK2aXHscJkh\GWlcnXhd4V{KHOxZHn1cUB{\Wynbnn0[U1qdmS3Y3XkJIFxd3C2b4Ppdy=>NX3s[lJwOjJ5MkG4NFQ>
Primary OPCNEHEZ25CeG:ydH;zbZMhSXO|YYm=NXHPSIxPOc7:TR?=NHjJW3c3cC9{NHi=MnvRdoVlfWOnczD0bIUheGW{Y3XueIFo\SCxZjDj[YxteyCrbjDlZZJtgS1iYX7kJIxifGVvYYDvdJRwe2m|L37lZ5Jwe2m|MW[yNlcxPzN6NR?=
PMNsM4LZOmFxd3C2b4Ppd{BCe3OjeR?=Mlq4OFDPxE1?MnO3Oog>NXfVTI95TE2VTx?=M2fsdpJmfmW{c3XkJJRp\SCjbX;1cpQhd2ZiY3zlZZZm\CClYYPwZZNmNTNidH:gcoVieiC4ZXjpZ4xmKGyndnXsdy=>MVSyNlY6OjV5Nx?=
A549 NWSyNJVXSXCxcITvd4l{KEG|c3H5NFrW[2w2OM7:TR?=NF25NGEycA>?M3zxTZBz\X[nboTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEKjb3j1c5Nq\GViSR?=M{G1cFIzPjh5NkO1
AGSMUTBdI9xfG:|aYOgRZN{[Xl?M{LiW|Ix|ryPNFfnbZUyOmh?MYXpcohq[mm2czD0bIUh[WO2aY\heIlwdiCxZjDwdo8u[2G|cHHz[U0{KGmwIILld5BwdnOnIITvJJRp\SCHdF;BZ{BnemGldHnvci=>MU[yNlY5PzN7OB?=
shC9 Mn24RZBweHSxc3nzJGF{e2G7MVyxNO69VQ>?M4OwR|E3cA>?NFrpeFRjdHWwdIOgV2hJKGW6cILld5Nqd25iaX6gd4hEQSClZXzsd{BmgHCxc3XkJJRwKGWrdHjldkBRSSCxcjDMVGM>MUKyNlY1OTB7NB?=
primary MEFsMVLGeY5kfGmxbjDBd5NigQ>?M2H3N|EzcA>?MoTFxsBqdmO{ZXHz[ZMh\XSxcH;zbYRmNWmwZIXj[YQhdWm2b3Poc45lemmjbDDt[Y1jemGwZTDk[ZBwdGG{aYrheIlwdg>?MnTuNlI3OTN5Nke=
3T9 MEFsMon4SpVv[3Srb36gRZN{[Xl?MVuxOog>MXzpcoNz\WG|ZYOgZ5l1d2Oqcn;t[UBkKHKnbHXhd4Uh[W[2ZYKg[ZRweG:|aXTlMZRz\WG2bXXueC=>M4\v[VIzPjF|N{[3
3T9 MEFsMn30SpVv[3Srb36gRZN{[Xl?MV2xPIg>M3G4RZVxemWpdXzheIV{KGmwaYTpZZRweiClYYPwZZNmNTliYoX0JIRwf26{ZXf1cIF1\XNiZX\m[YN1d3JiY3HzdIF{\XNiYX\0[ZIh\XSxcH;zbYRmKHS{ZXH0cYVvfA>?MkjaNlI3OTN5Nke=
MDA-MB-231 MXTBdI9xfG:|aYOgRZN{[Xl?NH\TOYUzOM7:TR?=NV3WW2hLPGR?M{nCOJJm\HWlZYOgeIhmKGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkhdWW2Zn;ycYlvKHS{ZXH0cYVvfCC5YYOgd4lodmmoaXPhcpRtgQ>?NWqyU5dpOjJ3OUO0OFE>
C6 MXXBdI9xfG:|aYOgRZN{[Xl?MmLKNVDPxE1?MnXMNlRpNXftS4tk[myxY3vzJJRp\SC|dYDwdoV{e2m4ZTDl[oZm[3Rib3[geIhmKHCncITp[IUhd25idnnhZoltcXS7MXuyNlU5QDl6MB?=
HL-60 NV\pfmwxSXCxcITvd4l{KEG|c3H5NGr0Z3gyODEQvF2=MViyOIg>NX3rWmVPcW6qaXLpeJMhfGinIHPlcIwh[XCxcITvd4l{KHOnbHXjeIVlKChtKT3t[Y51cHmuIN8yMUgy6obUNjmt[IlodHWlb4D5doFvd3OrZHZCpFU>NHrrbFUzOjV2Nk[2PS=>
HL-60NXXUTGxxSXCxcITvd4l{KEG|c3H5M13kfFExNThyzszNMVG0bC=>MV7pcohq[mm2czDUS2hSNWmwZIXj[YQh[2WubDDhdI9xfG:|aYRCpC=>NInNdYQzOjV{M{KyPS=>
BCCNXLxblV5SXCxcITvd4l{KEG|c3H5MX[1NO69VQ>?M3rp[lFpM3[x[olvcGmkaYTzJGRCXFNvbXXkbYF1\WRiZ4Lve5RpKGmwaHnibZRqd25?MVWyNlUyQTR|Nh?=
RAW 264.7NGDRdmxCeG:ydH;zbZMhSXO|YYm=MXe1NE8yODEQvF2=MY[xbC=>NEnsTGtkd26lZX70doF1cW:wLXTldIVv\GWwdDDpcohq[mm2czDEU24ucW6mdXPl[EBzWk6DIHPs[YF3[WenMle5NlI1QTF2Mk[=
K562MkC4RZBweHSxc3nzJGF{e2G7NHrKe3QzPc7:TR?=MmPUNog>NHnwOIhxemW4ZX70d{BieG:ydH;zbZMhcW6mdXPl[EBjgSClbz30doVifG2nboSge4l1cCCjbYXy[Y5{cW5iRzDhcoQhXFKDSVy=MnuyNlI1QDN5N{e=
SGC-7901NHLodFhCeG:ydH;zbZMhSXO|YYm=M3nhXlIxKM7:TdMgM3HnNFJpMk\QZZR1\W63YYTld{BJOk9{wrDvdkBVVkZizsGtbY5lfWOnZDDj[YxtKGGyb4D0c5NqeyCjczD3[YxtKGG|IHPhd5Bie2VvMzDhZ5Rqfmm2edMgMmrPNlI1PzF3OEm=
PC3NIDF[JhCeG:ydH;zbZMhSXO|YYm=MVGxNO69VQ>?MoL1OIg>M3XhSYNwfW62ZYLzJIZt[X[xY3;4bYQucW6mdXPl[EBk[XOyYYPlMZJmdGG2ZXSgZZBweHSxc3nzMUeyNlQ4OTl5NB?=
SMMC-7721NEXXPW5CeG:ydH;zbZMhSXO|YYm=MY[1NO69VQ>?M1fzPFQ5cA>?NUTEd2ps[XS2ZX71ZZRmeyCxVGKtbY5lfWOnZDDhdI9xfG:|aYO=M{e4[lIzPDZ3OEOz
HeLa MWDBdI9xfG:|aYOgRZN{[Xl?MnPHOVDDqM7:TR?=NYTYXG92PC96aB?=MmLybY5pcWKrdIOgV3RUNWmwZIXj[YQhdGG2ZT3wbIF{\SCjcH;weI91cWNiZY\lcpR{NUjLR4RFOjJ2NkC1NFQ>
HeLa NUH3e4dvSXCxcITvd4l{KEG|c3H5MmHYOVDDqM7:TR?=NUTMTG1bOWh?MoTDd5VxeHKnc4Pld{B1cGViRmLBVE1qdmS3Y3XkJIFk[3WvdXzheIlwdiCxZjDhco5mgGmwIG[gdI9{cXSrdnWgZ4VtdHN?M1fRW|IzPDR7NESw
T47D MY\BdI9xfG:|aYOgRZN{[Xl?M1fkVlExOM7:TR?=NVi1T5pZOWh?M3i0NoJtd2OtczD0bIUh\2WwZYLheIlwdiCxZjDFMYNi\C:FVF[yJIJ6KFOWUx?=Mly1NlI1ODFzNki=
HeLa M2XsO2Fxd3C2b4Ppd{BCe3OjeR?=M3LjTVMx|ryPM4[yXFRpM{jDfuKhcW6lcnXhd4V{KHSqZTDn[Y5memGuIHPlcIwhfmmjYnnsbZR6KDR6IHigZYZ1\XJicHjveI9lgW6jbXnjJJRz\WG2bXXueOKhMmjKNlI{QTR{NEi=
HCCM321NmFxd3C2b4Ppd{BCe3OjeR?=NVvkOG16OjBizszNxsA>M3jnTlJpMnHTZZR1\W63YYTld{B1cGViRFjBJIlv\HWlZXSgZYN1cX[jdHnvckBw\iCSQWLQM4W0TVIzOzR{N{Oy
mESCsNXWyPWI4SXCxcITvd4l{KEG|c3H5Mnz5Nk42|ryPNWjOcFBrOmh?MnPNbY5pcWKrdIOgeIhmKE6jRj3t[YRq[XSnZDDjZZNx[XOnIHHjeIl3[XSrb36=MknmNlIzQDV{N{S=
EMT-6 MUPDfZRwfG:6aXPpeJkhSXO|YYm=NX3heXp5OTByzszNMV[xbC=>NVzsbXFSeGG{dHnhcIx6KGKub3Pr[YQh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IIPpdoFu\XOrbnW=MUWyNlI2OTl{MR?=
MCF7M33IPWFxd3C2b4Ppd{BCe3OjeR?=NF3PT2E2OMLizszNM{TtWVFpM2TDNolvcGmkaYTzJHBCKGGldHn2ZZRm\CClYYPwZZNmNTNuID25MEBidmRicH;sfUhCTFBvcnnic5NmMSCyb3z5cYVz[XOnMorpNlIzOjN|NEW=
K562MUTBdI9xfG:|aYOgRZN{[Xl?M4rBTFIxKM7:TdMgNFLNdJU1QGh?M{DTU2ROW09?NYnXboVy[myxY3vzJIxieGG2aX7pZk1qdmS3Y3XkJIlvcGmkaYTpc44hd2ZidnnhZoltcXS7IHHu[EBieG:ydH;zbZMhcW6mdXP0bY9vMmDINlIzOTZzNUi=
Molt4-hygNYDXR5p2SXCxcITvd4l{KEG|c3H5MnjRNVDPxE1?MoPxNE42cA>?M3jZVIJtd2OtczDmZZJv\XOxbD3pcoR2[2WmIHPhd5Bie2VvMz3sbYtmKGGldHn2bZR6NUjIfGlpOjZ{N{W4NVE>
HeLaMUfBdI9xfG:|aYOgRZN{[Xl?NHPQWXYyOM7:TR?=NX\2c3BnOC53aB?=MlnJbY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkhfGinIHPvcYJqdmWmIITy[YF1dWWwdDD3bZRpKGexbXnzbY4hViCjbnSgWHJCUUx?NHrNZVMzOjF5OU[2NS=>
Jurkat TM1rESGFxd3C2b4Ppd{BCe3OjeR?=NHLpfmk{OM7:TR?=MknwNE42cA>?NV3DNJdRTE2VTx?=NEPRTWVjdG:la4OgeIhmKHqrcnHtMYlv\HWlZXSgZZBweHSxc3nzNFTDWIUzOjF3OUi5PC=>
NeutrophilNY\4U4ZVSXCxcITvd4l{KEG|c3H5MnTXNlDPxE1?NVzrelF3OC53aB?=M3PlTYF1fGWwdXH0[ZMhfGinIIDyc{1ieG:ydH;0bYMh\W[oZXP0JI9nKE2jUkG=MXyyOlE6Pjh2NB?=
HCT116MVPBdI9xfG:|aYOgRZN{[Xl?NVPtOXptPTEEoN88US=>NEm2T4kzcA>?Mn\JdoV3\XK|ZYOgd5lv\XKpaYP0bYMh[XCxcITvd4l{KGWoZnXjeJMhd2ZiY3Xs[YNwgGmkIHHu[EBPWENvMUdCpC=>MXiyNlE2QTd3Mh?=
MDA-MB-231 NGXJTYZCeG:ydH;zbZMhSXO|YYm=NULkSplTOi53LUeuOe69VQ>?Mk\1Nog>MWnpcohq[mm2czD0bIUh[2WubDDk[YF1cCCxZjDNSGEuVUJvMkOxJINmdGy|IHnu[JVk\WRiYomgV2RVKGmwIHGgZ49v[2WwdILheIlwdiCmZYDlcoRmdnRibXHucoVzMUWyNlEyPTV{Nh?=
LNCaPNIjvR|hCeG:ydH;zbZMhSXO|YYm=NYPlemF4PDEQvF2=M3T0SFJpMlXpbY5pcWKrdIOgZpV1\WmwIHnu[JVk\WRiY3XscEBieG:ydH;zbZM>MUmyNlEyPDd4NB?=
MB231M1nFN2Fxd3C2b4Ppd{BCe3OjeR?=M1nNblExOM7:TR?=MmC2NYg>NFTuTYVFVVORMVnhZpJw\2G2ZYOgeIhmKGmwZIXjeIlwdiCxZjDj[YxtKGSnYYToJIJ6KFeHRUGgbY5pcWKrdHnvckwhXFKDSVygeJJm[XSvZX70MEBidmRidHjlJINwdWKrbnH0bY9vMUmyNlEyOjl2MB?=
HCC38 NWLMW|lJSXCxcITvd4l{KEG|c3H5NYX5cFJ[OTByzszNNXH0dJB[OWh?MUTEUXNQMl7PZYJzd2ejdHXzJJRp\SCrbnT1Z5Rqd25ib3[gZ4VtdCCmZXH0bEBjgSCZRVWxJIlvcGmkaYTpc44tKFSUQVnMJJRz\WG2bXXueEwh[W6mIITo[UBkd22kaX7heIlwdg>?NWnCeVY{OjJzMUK5OFA>
MDA-MB231MkPERZBweHSxc3nzJGF{e2G7NFnHfG02OMLizszNNWPib2UzOjSqM3vNTGROW09?MljyZYJzd2ejdHXzJIN2emO3bXnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=>M2exeVIzOTBzM{O1
LNCaPMnPRRZBweHSxc3nzJGF{e2G7NHj4Vng2OMLizszNMXiyOIg>MU\EUXNQMlrDZYJzd2ejdHXzJIN2emO3bXnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=>NUTaWmtQOjJzMEGzN|U>
HCT116M4C1SWFxd3C2b4Ppd{BCe3OjeR?=MkWwOVDDqM7:TR?=MYKyOIg>M1zjOmROW09?M{fsboFjem:pYYTld{BkfXKldX3pck1qdmS3Y3XkJINmdGxiZHXheIg>M2HJNlIzOTBzM{O1
Ishikawa M2rJR2Fxd3C2b4Ppd{BCe3OjeR?=MlWzNlXPxE1?MVeyOIg>M{K3dZJm\HWlZYOgZ4VtdCCmZXH0bEBidmRiYYDvdJRwe2m|IHnu[JVk\WRiYomgRoFnKEFzNIj3fpYzOjB6OEmxPC=>
 YD-8 M1z3ZmFxd3C2b4Ppd{BCe3OjeR?=MUSxNFDPxE1?NXrycXk1OWh?NHjDT5hqdmirYnn0d{BRVEWRLXnu[JVk\WRiYYDvdJRwe2m|NHfze2gzOjB6NkG4Ny=>
eosinophil MV;BdI9xfG:|aYOgRZN{[Xl?MknmPVDPxE1?MlnENlRpMk\CbY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkhTmG|IHHueIljd2S7wrC=M2HhXVIzODd7M{O0
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YD-8 MXvBdI9xfG:|aYOgRZN{[Xl?NULnOIVyOTByzszNNWOyW3A{OWh?NXXQU5R4[myxY3vzJJRp\SCJUz3IR4wucW6mdXPl[EBieG:ydH;zbZM>MYKyNlAzODB5OB?=
HBxNHniVlBCeG:ydH;zbZMhSXO|YYm=NF70fHEzPc7:TR?=M1TMe|Q5cA>?M1fJT2ROW09?NIrRSGJz\WS3Y3XkJINmdGxiZHXheIghcW6mdXPl[EBjgSB|LV3BM1fNfFIzODJyMEe4
U937 NEK0eYRCeG:ydH;zbZMhSXO|YYm=NWPCV|h4PTEEoN88US=>NXPlWWNEOWh?NYnzWmV6cW6qaXLpeJMhUEZvaX7keYNm\CCjcH;weI9{cXQEoB?=NX3ofnlNOjF7OUi3N|E>
HL60M13CSGFxd3C2b4Ppd{BCe3OjeR?=MUe0NO69VQ>?MYC0NI1qdg>?NYSyZXNyTE2VTx?=MnjkZoxw[2u|IFLOSGMh[2:vcH;1coR{KGmwZIXj[UBmgHCxc4Xy[UBw\iCyaH;zdIhifGmmeXzz[ZJqdmViYX7kJGRPSSCocnHncYVvfGG2aX;uMXWyNVk5OzJ7Nh?=
M-14NXzIe5U3SXCxcITvd4l{KEG|c3H5NGXP[YszPc7:TR?=MW[wMlVpMkX4xsBqdmirYnn0d{Bjd3SqIITo[UBkenWmZTDlfJRz[WO2LTDhcoQh[2:vcH;1coQucW6mdXPl[EBieG:ydH;zbZM>MlKzNlE6PTR7NUm=
SK-BR-3MnnVRZBweHSxc3nzJGF{e2G7NUP2VGRxPTEEoN88US=>MoT4Nog>M1HXbYJtd2OtczDhdI9xfG:2aXOgSG5CKG[{YXft[Y51[XSrb36gbY5lfWOnZDDifUBkd22kaX7heIlwdiCxZjDTUU0yPjRiYX7kJJJi\GmjdHnvci=>MX6yNVkxOTN6Nh?=
MDA-MB-468MlO5RZBweHSxc3nzJGF{e2G7MU[1NOKh|ryPMX2ybC=>MVTicI9kc3NiYYDvdJRwfGmlIFTORUBnemGpbXXueIF1cW:wIHnu[JVk\WRiYomgZ49u[mmwYYTpc44hd2ZiU12tNVY1KGGwZDDyZYRq[XSrb36=MYGyNVkxOTN6Nh?=

... Click to View More Cell Line Experimental Data

In vivo
Features A key compound for apoptosis studies.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDog
Weight (kg)0.020.151.80.40.0810
Body Surface Area (m2)0.0070.0250.150.050.020.5
Km factor36128520
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)
1

References

[1] Slee EA, et al. Biochem J, 1996, 315 (Pt 1), 21-24.

[2] Shimizu T, et al. Leukemia, 1997, 11(8), 1238-1244.

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Chemical Information

Download Z-VAD-FMK SDF
Molecular Weight (MW) 467.49
Formula

C22H30FN3O7

CAS No. 187389-52-2
Storage 3 years -20℃powder
6 months-80℃in solvent
Synonyms N/A
Solubility (25°C) * In vitro DMSO 93 mg/mL (198 mM)
Water <1 mg/mL (<1 mM)
Ethanol 24 mg/mL warming (51 mM)
DMF 84 mg/mL
* Z-VAD-FMK will racemize in DMSO, water and ethanol.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name L-Alaninamide, N-[(phenylmethoxy)carbonyl]-L-valyl-N-[(1S)-3-fluoro-1-(2-methoxy-2-oxoethyl)-2-oxopropyl]-

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Tel: +1-832-582-8158 Ext:3

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