Z-VAD-FMK

Catalog No.S7023

Z-VAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.

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Z-VAD-FMK Chemical Structure

Z-VAD-FMK Chemical Structure
Molecular Weight: 467.49

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Product Information

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Product Description

Biological Activity

Description Z-VAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor, blocks all features of apoptosis in THP.1 and Jurkat T-cells.
Targets Pan-caspase [1]
(THP.1, Jurkat T-cells)
In vitro Z-VAD-FMK (10 mM) inhibits apoptosis in THP.1 cells. Z-VAD-FMK (10 μM) inhibits activation of PARP protease activity in control THP.1 cell lysates. Z-VAD-FMK (10 mM) inhibits the processing of CPP32 in intact THP.1 and Jurkat cells. [1] Z-VAD-FMK (50 μM) cotreatment abolishes the apoptotic morphology of camptothecin-treated HL60 cells. Z-VAD-FMK (50 μM) blocks camptothecin-induced DNA fragmentation in HL60 cells. [2] Z-VAD-FMK (50 μM) inhibits cell death following dSMN dsRNA-induced apoptosis in S2 cells. Z-VAD-FMK (50 μM) increases the percentage of transfected cells surviving from 26% to 63% in S2 cells. [3] Z-VAD-FMK (> 100 μM) enhances TNFα-induced neutrophil apoptosis, lower concentrations (1-30 μM) completely blocks TNFα-stimulated apoptosis in human neutrophils. [4] Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes. Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes detected with the TUNEL assay. [5]
Cell Data
Cell LinesAssay TypeConcentrationIncubation TimeFormulationActivity DescriptionPMID
GC1aM2DyWGFxd3C2b4Ppd{BCe3OjeR?=MYW1NO69VQ>?MXuyOIg>M3rNUolv\GmlYYTld{BiKHC{b4TlZ5RqfmViZX\m[YN1KGGpYXnud5Qh\XSxcH;zbYRmNHTzWpAzPjF4OUC3OS=>
HGL5MVTBdI9xfG:|aYOgRZN{[Xl?MUG1NO69VQ>?MmLENlRpNWixVW9mcW6maXPheIV{KGFicILveIVkfGm4ZTDl[oZm[3RiYXfhbY5{fCCndH;wc5Nq\GV?NW\GPXh5OjZzNkmwO|U>
HepG2M3nKW2Fxd3C2b4Ppd{BCe3OjeR?=M4jXcVIx|ryPMWixbC=>MorHZZR1\W63YYTl[EB1cGViYYDvdJRwfGmlIHnu[JVkfGmxbjDv[kBKUUlvMUFCpC=>MX[yOlE3PDd7NR?=
BEL-7402NUXHZlhoSXCxcITvd4l{KEG|c3H5NYnZOnp7OjEQvF2=NGrFdIUycA>?MUjheJRmdnWjdHXkJJRp\SCjcH;weI91cWNiaX7keYN1cW:wIH;mJGlKUS1zMNMgMWmyOlE3PDd7NR?=
CEFNFjvXYJMcW6jc3WgRZN{[Xl?NVWzSFJGOCx|Mzy2O{wyODEQvF2=MUOxOY1qdg>?MV7kc5dvNXKnZ4XsZZRmeyCSUjDlcpp6dWViYXP0bZZqfHlidH:gOFDwxIViYYSgNVAx|ryPMkW3NlYyODJ|M{m=
SP2/0Ml:yRZBweHSxc3nzJGF{e2G7MnjWNVAx|ryPNX32OVlTOWh?MmTBSG1UVw>?NXLwNndm[myxY3vzJJRp\SCjcH;weI9{cXNib3[gV3AzNzBiY3XscJM>MXeyOlA4PDd|Mh?=
HUVEC-2cMn\xRZBweHSxc3nzJGF{e2G7NH3XZWc2OM7:TR?=NHPrVIo3cA>?NGXE[JBl\WO{ZXHz[YQhfGinIH;4MWxFVC2rbnT1Z4VlKGG3dH;wbIFogQ>?NGDi[I8zPjB{MUeyPS=>
U1NWe1OmlKSXCxcITvd4l{KEG|c3H5NXjDcXFSOC1zMEFOwG0>NEK1e3kzcA>?M4P1[ZJm\HWlZYOg[JJ2\y2rbnT1Z4VlKGGyb4D0c5NqeyCjbnSgd5Vje2WzdXXueEBJUVZvMTDy[ZBtcWOjdHnvckBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=>MX2yOVk5ODl2Mh?=
ACH-2MYHBdI9xfG:|aYOgRZN{[Xl?M4DYT|AuOjByzszNMViybC=>M4rtXJJm\HWlZYOg[JJ2\y2rbnT1Z4VlKGGyb4D0c5NqeyCjbnSgd5Vje2WzdXXueEBJUVZvMTDy[ZBtcWOjdHnvckBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=>MnvhNlU6QDB7NEK=
U1Mn;nT4lv[XOnIFHzd4F6NGKxNXkyODEQvF2=NXXZOGNsOmh?MljnbY5pcWKrdIOgZ4F{eGG|ZT2zMn7vNlU6QDB7NEK=
A549/V16M4XrdGFxd3C2b4Ppd{BCe3OjeR?=NIf3TFA2OM7:TR?=MnHZNYg>NXfSNoRKemWycnXzd4V{KFSJL2TNMYlv\HWlZXSgZZBweHSxc3nzMmDrNlU6PDZyM{O=
SGNNEXNNJlCeG:ydH;zbZMhSXO|YYm=M3r3fVIxdU1?NFziVJY1QGh?NVrVO|hxcGG|IH7vJIlv\my3ZX7j[UBwdiCDSV[sJINidHCjaX6g[ZhxemW|c3nvckBweiClZXzsJIFxd3C2b4Ppdy=>NXTQdmxMOjV6N{S2N|M>
HCT116 Mof6RZBweHSxc3nzJGF{e2G7MkThOVDPxE1?MXSybC=>M1Po[olvcGmkaYTzJJRp\SClZXzsJIRm[XSqIHnu[JVk\WRiYomgSGRXWA>?MVWyOVg3QDhzOB?=
DTK-SMEMV\BdI9xfG:|aYOgRZN{[Xl?MYK1NO69VQ>?M{\iVFJpNGDrSFRFVVORMnL4dIFzfGmjbHz5JIlvcGmkaYTzJJRp\SCjcH;weI9{cXNiaX7keYNm\CCkeTDieZBqfmGlYXnu[S=>NEfrfVUzPTh2M{i5Oy=>
HL-60NXLtbGFpSXCxcITvd4l{KEG|c3H5M1vtcVUx|ryPMXG0PIg>NGrzTXNz\WS3Y3XzJINmdGxiZHXheIgh[2G3c3XkJIJ6KFONST3JTUB1emWjdH3lcpTDqA>?M{nUbVI2QDJ2MESz
U937NWjMeFA4SXCxcITvd4l{KEG|c3H5MX[1NO69VQ>?MUW0PIg>NEP0SmVz\WS3Y3XzJINmdGxiZHXheIgh[2G3c3XkJIJ6KFONST3JTUB1emWjdH3lcpTDqA>?MXWyOVgzPDB2Mx?=
A549MVnBdI9xfG:|aYOgRZN{[Xl?NVj5UZI4OjBizszNM{CyXFI1cA>?NGKwSo1z\WS3Y3XzJINmdGxiZHXheIgh[2G3c3XkJIJ6KEiIQ2CgeJJm[XSvZX70MXSyOVc6PDF2OR?=
HeLaM{fYTWFxd3C2b4Ppd{BCe3OjeR?=M1zOblEx|ryPM1fLflQ5cA>?NFjpUJRl\WOuaX7ld{B1cGVicnH0[UBw\iCjcH;weI9{cXNiZILhcYF1cWOjbHz5MVKyOVc4OjV2NR?=
SGC-7901NEjweYZCeG:ydH;zbZMhSXO|YYm=M3zmflIxyqEQvF2=NHLUfoYycA>?NXjGTVRkcW6qaXLpeJMhfGinIHPlcIwh\GWjdHigbY5lfWOnZDDifUBwgGGuaYDsZZRqdg>?MnG3NlU4PjdyN{[=
MCF-7Mn\xRZBweHSxc3nzJGF{e2G7MnfiNVDPxE1?NE\uNXIzcA>?MYLpcohq[mm2czD0bIUh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IEPCNI[4dlgzPTd{MkGxOC=>
A549NFPGXnFCeG:ydH;zbZMhSXO|YYm=M1:zT|ExKM7:TR?=MkjXNlRpMUXEUXNQNHvQRZVxem:vb4Tld{BJSkNvdILlZZRm\CCDNUS5JINmdGxic4Xyeol3[WxiJjDheJRmdnWjdHXzJJRp\SClbHXheoVlKFCDUmCg[ZhxemW|c3nvci=>M{fHbVI2Pjh|NU[4
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Neocortical NeuronMW\O[ZVzd3SxeHnjbZR6KEG|c3H5NUTsOmdkOTByzszNNHrBUYkycA>?NX\SSIpx[W62YXfvcol7\XNiSH;pZY1q\GViQT3JcoR2[2WmIF7leZJwfG:6aXPpeJk>MmXaNlU3PzVyMEG=
Primary human placental cytotrophoblastsMl;zRZBweHSxc3nzJGF{e2G7MnLMN|DPxE1?M3rpdVI1cA>?NV7XS2k1TE2VTx?=MkXCdoV3\XK|ZYOgeIhmKGmwaHnibZRqd25ib3[gNVHPui2KU1SyJIJ6KHS{aXPsc5NidsLiMn;jNlU3PDJ3OUK=
MMMnvERZBweHSxc3nzJGF{e2G7NGf3NYs2OM7:TR?=MoPLNlBucW5?NIj4emhx[XK2bImgbY5pcWKrdIOgV2hMNWmwZIXj[YQhKGOnbHyg[IVifGh?NFvtSGEzPTV|MEC5PC=>
DLD1NGfoXlhCeG:ydH;zbZMhSXO|YYm=MoXCNlDDqM7:TR?=NYjoSFROOWh?MWnwZZJ1dHlicnX2[ZJ{\XNiY3XscEBieG:ydH;zbZMh[2G3c3XkJIJ6KFeVUEG=MUCyOVUzPDJ2Nh?=
MDA-MB-231NGDQZnREgXSxdH;4bYNqfHliQYPzZZk>NXzx[WMyOTEQvF2=M3zMdlFpM1yyNIF2\22nboTzJINmdGxiZHXheIgh[W[2ZYKgR2hWSSC2cnXheI1mdnR?MY[yOVUzOTVyMR?=
MKN28MV3BdI9xfG:|aYOgRZN{[Xl?NGS2dIYyOM7:TR?=MXSzNI1qdg>?NWS3Z2htTE2VTx?=NVvkR2NjcW6qaXLpeJMhXE6ILd8xJJBtfXNiQ1jYMYlv\HWlZXSgZZBweHSxc3nzMYOyOVUyOzl4MB?=
HL-60M2PldWN6fG:2b4jpZ4l1gSCDc4PhfS=>M2P6WFExOM7:TR?=NWDZTHNlOWh?MY\EUXNQM1;h[uKhcW2ycn;2[ZMhfmmjYnnsbZR6KG:oIGTDUmF{NXS{ZXH0[YQh[2WubIO=NUHIRpREOjV3MEK5N|I>
AGSNHntb4VCeG:ydH;zbZMhSXO|YYm=Ml7WNVDPxE1?MXmxbC=>MWPwdoV3\W62czDjeZJkfW2rbj3pcoR2[2WmIHPs[YF3[WenIH;mJINie3Cjc3WtN{whNThuIHHu[EAuQSCycn;0[Ylvew>?NGnZVJgzPTR7MkKxOC=>
A549 Ml24RZBweHSxc3nzJGF{e2G7NU\QZ5RZOTEQvF2=NFvONI0zPGh?NHexfIxjdG:la4OgSG1CWy2rbnT1Z4VlKGOuZXH2ZYdmKG:oIHPhd5Bie2VvMzDhcoQhWEGUUDDhcoQh[XCxcITveIlkKGOnbHyg[IVifGh?M3u2Z|I2PDN2OUi5
INS-1MnnZRZBweHSxc3nzJGF{e2G7MkTROVDPxE1?NUXpT5p6Pmh?MnTLSG1UVw>?MVHk[YNz\WG|ZYOgeIhmKHC{b4DvdpRqd25ib3[g[YFzdHliYYDvdJRwfGmlIHPlcIx{yqB?NXLYWlJjOjV2M{C4PVc>
Primary hepatocytes NUfOcZc2SXCxcITvd4l{KEG|c3H5MoHzOVDPxE1?NHu0O44yQGh?NYHCfmNUcW6qaXLpeJMh[XCxcITvd4l{KG:oIHjldIF1d2O7dHXzJIlv\HWlZXSgZpkhSWO2IFSgZY5lKFSQRj5OtS=>MmexNlU1ODd3M{i=
L929-A MlrSR5l1d3SxeHnjbZR6KEG|c3H5NXzsZmVnOjEEoN88US=>Mmq5NlRpMUTpcohq[mm2czDUUmbPuS2rbnT1Z4VlKGOnbHyg[IVifGh?MnPMNlU{QTh3NEC=
L929-NNYjEbY9mS3m2b4TvfIlkcXS7IFHzd4F6MkjENlDDqM7:TR?=NVfP[XR1OjSqMmXm[Y5p[W6lZYOg[IVifGhidnnhJIF2fG:lcnnu[UBVVkcQsTDwdo9lfWO2aX;uNGfqPJUzPTN7OEW0NC=>
Cytotoxicity AssayMnPxR4VtdCCYaXHibYxqfHliQYPzZZk>NIDWVlEzOM7:TR?=NXzSNJB5PDiqM4TrWpBz\X[nboSgUWhOTC2rbnT1Z4VlKGOnbHyg[IVifGh?MoL5NlU{QTJzMU[=
COS7NI\tbY1McW6jc3WgRZN{[Xl?NYDCOW1iOTEQvF2=M1nFTFI1cA>?NEfu[GhqdmO{ZXHz[ZMh[2G|cHHz[UA{NzdiYXP0bZZqfGmncx?=M2C1V|I2Ozh2MEK1
COS7NEjUOlZCeG:ydH;zbZMhSXO|YYm=NYH5bmNlOTEQvF2=Mo[zOFhpMki4dIFzfGmjbHz5JJBz\X[nboTl[EBHSzFyMT3pcoR2[2WmIHPlcIwh\GWjdHi=M2mwcVI2Ozh2MEK1
A375MXXBdI9xfG:|aYOgRZN{[Xl?MkXCN|DPxE1?NInoc3UzcA>?NGXEboVxemW4ZX70d{B1cGViZIL1[{1qdmS3Y3XkJHBCWlBiY3zlZZZi\2V?MoP4NlU{PzZzMUW=
A549NH;DOI5CeG:ydH;zbZMhSXO|YYm=M4TXV|Ux|ryPMVmyOIg>M1\rcJJmfmW{c3XzJJJq[m:|b33lJIJqd2enbnXzbZMh[W6mIHHwc5B1d3OrczDjZZV{\WRiYomgJGNpcWqxbnfkZY4>MmThNlU{PDl5OEG=
A549NIfFVZBCeG:ydH;zbZMhSXO|YYm=MXmyMlUuOjYQvF2=NUHXTlFuOWh?NUWxNmlJ\GWlcnXhd4V{KHSqZTDwc5B2dGG2aX;uJI9nKGGyb4D0c5Rq[yClZXzsd{Bl\XCnbnSgc44h[2:wY3XueJJifGmxboO=NE\0PJEzPTN2MkSyOy=>
 NBL-W-S MUDBdI9xfG:|aYOgRZN{[Xl?MkTZOVDPxE1?M4Wz[lFpNYDqRY9Y\nWubImgdoV{[3WnczDj[YxtKH[rYXLpcIl1gSCjZoTldkBISU6WLU[xJJRz\WG2bXXueC=>NHLDc4UzPTN{M{KyNi=>
Caki-1 NUPlXoJMSXCxcITvd4l{KEG|c3H5MW[0NO69VQ>?NFn5PHo1QGh?M3vlcJJm\HWlZYOgeIhmKG63bXLldkBw\iCDbn7lfIlvKFZvcH;zbZRqfmViY3XscJM>NY\ENlU4OjV{N{mxPVE>
769-PMXzBdI9xfG:|aYOgRZN{[Xl?NXzqTFZyPDEQvF2=MYC0PIg>MWPy[YR2[2W|IITo[UBvfW2kZYKgc4YhSW6wZYjpckBXNXCxc3n0bZZmKGOnbHzzNY\0c2RxOjV{N{mxPVE>
H9c2MWLBdI9xfG:|aYOgRZN{[Xl?M17BcFUx|ryPM{[1Z|FpNYjyUnFCcW6qaXLpeJMhTE:[LXnu[JVk\WRiY3HzdIF{\SB|IHHjeIl3[XSrb36gZpV1KG6xdDD0bIUhdG:|czDv[kBk\Wyucx?=M4[yVlI2Ojh|OEG5
K562Mk\JRZBweHSxc3nzJGF{e2G7M{[5fVUx|ryPNUS1bpc2PGh?Ml3BbY5pcWKrdIOgToFkNUFvaX7keYNm\CClZXzsJIFxd3C2b4Ppdy=>NVH3S5VMOjV{NEG2NVk>
L929Mkf4RZBweHSxc3nzJGF{e2G7M3zHd|Ex|ryPMXe2bC=>NYCwUZZPcW6mdXPld{Bv\WO{b4D0c5NqeyC5aYToJHRPTg>?M3\5S|I2OTl3Nk[w
A549NIrL[mlCeG:ydH;zbZMhSXO|YYm=MV[xNO69VQ>?M{Pmb|NpNGHROIdz\WS3Y3XzJINmdGxiYYDvdJRwe2m|IHPheZNm\CCkeTDQVXE>M4rNblI2OTZzNkm5
MCF-7NHPWeIlCeG:ydH;zbZMhSXO|YYm=M13sNVIx|ryPMU[ybC=>NVHxVmJW\GWlcnXhd4V{KEuGUj3zbXJPSS2rbnT1Z4VlKGGyb4D0c5Nqew>?NYW0N201OjVzOEKyOFA>
OSNWrrUGdXSXCxcITvd4l{KEG|c3H5M2PON|IxNzRyzszNNW[4bHMyPzKqM4n0eYlvcGmkaYTzJG9UKGOnbHygeoli[mmuaYT5JJJm\HWldHnvckBjgSCFNjDj[ZJidWmmZR?=M4rtXlI2OTV{M{m5
HUVECsMXTBdI9xfG:|aYOgRZN{[Xl?NIHGVI8yOM7:TR?=NEXSfoQzcA>?NYnLOJE2yqCrbnjpZol1eyCjcH;weI9{cXNiYX7kJIZi[2muaYTheIV{KGG3dH;wbIFogSCrbjDESW5XOi2rbn\lZ5Rm\CCKVW\FR5M>M3S3ZlI2OTN6N{Cz
EA.hy926MnXtRZBweHSxc3nzJGF{e2G7MUWxNO69VQ>?M4THU|JpM1y0UOKhcW6qaXLpeJMh[XCxcITvd4l{KGGwZDDmZYNqdGm2YYTld{BifXSxcHjh[5khcW5iRFXOWlIucW6oZXP0[YQhTUFwaIm5NlY>NGnXPZgzPTF|OEewNy=>
EbsMlPpRZBweHSxc3nzJGF{e2G7M2TuW|ExNTFyMN88US=>MV:yOIg>NYr2cmJUyqCPTWTTJIdmdmW{YYTpc44hemG2ZTDk[YNz\WG|ZXSgZZMhfGinIHPvcoNmdnS{YYTpc44hd2Ziej3WRWQv\m2tIHnuZ5Jm[XOnZB?=MYGyOVE{PDhzNx?=
JurkatMluzRZBweHSxc3nzJGF{e2G7Ml\LNlDPxE1?M2X5fVI1cA>?NXm2XZo3TE2VTx?=M{XOTpBienSrYXzsfUBqdmirYnn0JINmdGxiZHXheIghd2ZiSoXyb4F1KGOnbHzzJIlv\HWlZXSgZpkhOTByNUitSlQh[2:vYnnu[YQhf2m2aDDWVGHDqA>?MYmyOVEzODd{Mx?=
K562MlzlRZBweHSxc3nzJGF{e2G7M4nj[lAvOS1zzszNMlvtNYg>NVX1c41IcW6qaXLpeJMh[2ynYY\h[4Uhd2ZiSGPQPVAhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI>MlHLNlUyOTlzOEi=
IMR-32MkLHRZBweHSxc3nzJGF{e2G7NH3FOWk1OMLizszNxsA>NFzQWGIzcA>?NGTS[pJl\WO{ZXHz[UBqdiCjcH;weI91cWNiY3XscJMh[2:vcHHy[YQhfG9iVD2yJJRwgGmwMV[yOVA5PDd3NR?=
RPMI 8226NILV[pVCeG:ydH;zbZMhSXO|YYm=NV;3dVN3OTByzszNMUCxbC=>M4S1WYFtdW:|dDDjc41xdGW2ZXz5JIJtd2OtczDj[YxtKGSnYYToJINifXOnZDDifUBv\WO{b4P0ZZRqdi1zNX7FRVV1OjVyM{e4OVE>
U266 NV\FVmlISXCxcITvd4l{KEG|c3H5MnTvNVAx|ryPMV2xbC=>MXjwZZJ1cWGubImgZoxw[2u|IHPlcIwh\GWjdHigZ4F2e2WmIHL5JI5m[3Kxc4TheIlvNTF?MnfaNlUxOzd6NUG=
H929 M{niR2Fxd3C2b4Ppd{BCe3OjeR?=MVyxNFDPxE1?NGf0b4kycA>?MoDTdIFzfGmjbHz5JIJtd2OtczDj[YxtKGSnYYToJINifXOnZDDifUBv\WO{b4P0ZZRqdi1zNHXzbVczPTB|N{i1NS=>
MM.1S MWrBdI9xfG:|aYOgRZN{[Xl?MlfvNVAx|ryPNYrYeoIxOWh?NUTy[mY1\W[oaXPp[Y51dHlicILleoVvfHNic3;yZYZmdmmkLXnu[JVk\WRiY3XscEBl\WG2aDDjc41jcW6nIIfpeIghdmWlcn;zeIF1cW5vMR?=MmL3NlUxOzd6NUG=
C6NEHxdotCeG:ydH;zbZMhSXO|YYm=NWrYNYloPTEQvF2=NHrhdI01QGh?Ml;XdJJmfmWwdIOgeIhmKGyxc4Ogc4Yh[2WubDD2bYFjcWyrdImgZ4F2e2WmIHL5JJBz\WewZX7vcI9v\Q>?MmDDNlUxOTN2N{m=
AGSMVzBdI9xfG:|aYOgRZN{[Xl?NXPEPGNoPTEQvF2=NFSxVpUzPGh?MorBZYJwdGm|aIOg{tIudGGyYXPoc45mNWmwZIXj[YQh[2WubDDk[YF1cCCjbnSgbY5pcWKrdHXkJIdzd3e2aB?=MUKyOVAxQTZ7OB?=
HeLa NUDJ[VViSXCxcITvd4l{KEG|c3H5M3y4SFQx|ryPMViyOIg>NWC5TI43cW6qaXLpeJMhfGinIHnuZ5Jm[XOnZDDhdI9xfG:|aYOgbY5lfWOnZDDifUB{cVKQRkGyNS=>MkD5NlQ6Ojh4OEW=
THP-1 M3q2WmFxd3C2b4Ppd{BCe3OjeR?=NHWwUWgyOM7:TR?=NIrhOYIycA>?Ml;UdoVlfWOnczDhdI9xfG:|aYOgbY5lfWOnZDDifUBCVEFvU1TUxsA>MVeyOFkzOzZ3Mx?=
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HeLa NV3CelRMSXCxcITvd4l{KEG|c3H5M3fyPFIx|ryPNYfDVJNjOmh?NIfyU2RqdmirYnn0d{BFVU2SIHnu[JVk\WRiYYDvdJRwe2m|M3LD[|I{QDZ|OU[2
U251NYq2bo86SXCxcITvd4l{KEG|c3H5NH7tVWwzOM7:TR?=M3HOXVI1cA>?MYnpcohq[mm2czDjZZN1cWOrbjDpcoR2[2WmIFeyM20heGijc3WgZZJz\XO2IHHu[EBieG:ydH;zbZM>Mo\hNlM5OTZ6MU[=
HL-60MkLhRZBweHSxc3nzJGF{e2G7MYW1NOK2VQ>?NGfUS4g1cA>?MXPicI9kc3NidHjlJINt\WG4YXflJI9nKGOjc4Dhd4UuOyxiLUmsJIFv\CCSQWLQJIlv\HWlZXSgZpkhPnN?NV3CdFR6OjN6MES3NFY>
Ec-109MUXBdI9xfG:|aYOgRZN{[Xl?M1XHS|ExKM7:TR?=NIXkPJY1QGh?NXzjXWxmemWycnXzd4V{KFCHLX3l[IlifGWmIFXjMVExQSClZXzsJIFxd3C2b4Ppdy=>MnLxNlM4QDJ4NEG=
RKOMn7pR4VtdCCYaXHibYxqfHliQYPzZZk>MmfFNVAh|ryPNUjUO2hlOjSqNIrtS4dqdmirYnn0d{B1cGViZHXjdoVie2Vib3[gZ4VtdCC4aXHibYxqfHliY3H1d4VlKGK7IFTBRi=>NF;pPGszOzd3OEC2OC=>
T98GNHvHPYVE\WyuIG\pZYJqdGm2eTDBd5NigQ>?Ml7MNU0yODEQvF2=M2i4VlI1cA>?NYTuNGhwcW2ycn;2[ZMh[2WubDD2bYFjcWyrdImgZ491emWjdH3lcpQhf2m2aDDUVy=>NUL0eohIOjN5NkmyO|U>
JurkatNIfnbI9E\WyuIG\pZYJqdGm2eTDBd5NigQ>?M2fMNlExOC1{MEFOwG0>NHfGZ|YzPGh?NXn5c4ZWcW6qaXLpeJMhUGGDNDDpcoR2[2W|IHHwc5B1d3OrczDk[ZBmdmSnboSgc4Yh[2:wY3XueJJifGmxbtMgNX\RS5A6OjN5M{K0PFE>
Molt-3 MoSxRZBweHSxc3nzJGF{e2G7NH\DZmI2OM7:TR?=M2X0clJpNYHRdoN2emWmdXPld{Bu\WyjdH;ubY4ucW6mdXPl[EBieG:ydH;zbZM>NWLvTlQyOjN5MkWwNVM>
hMSC12M13MNmFxd3C2b4Ppd{BCe3OjeR?=M2T4PFExOM7:TR?=NUnrbXB6PGR?NVz5R4ZqcW6qaXLpeJMhd3O2ZX;n[Y5q[yCldXz0eZJmNWmwZIXj[YQh[2WubDDk[YF1cCCjbnSgZ4Ft[2moaXPheIlwdg>?MVKyN|Y2Pzh{Mh?=
HM7 NES0Z5RCeG:ydH;zbZMhSXO|YYm=NVHKRnN3OjEQvF5CpC=>NX3VbJE4OWh?MmXPZoxw[2u|IHHwbYN2dGG{ZX6gRUBi[2W2YYTlMYlv\HWlZXSgZ4F{eGG|ZT2zJIFkfGm4YYTpc44h[W6mIGDBVnAh[2ynYY\h[4U>MmfTNlM2QDN2MUK=
Hep-2 NXTwUoRFSXCxcITvd4l{KEG|c3H5NVTuc41bOjEQvF5CpC=>MmHWNlRpMmfjSG1UVw>?NXjOPItW[WyuZY\pZZRmeyC2aHWg[IVkemWjc3Wgc4Yh[2WubDD2bYFjcWyrdImgbY5lfWOnZDDifUB{cWyrYnnubY4>Mkj0NlM2QDByM{K=
HSCsMV;BdI9xfG:|aYOgRZN{[Xl?MYC1NO69VQ>?NVjYcHg5OjSqMoTvSG1UVw>?M4nrZ4lvcGmkaYTzJI5qdG:2aX7pZk1qdmS3Y3XkJGRPSSCmYX3h[4UhcW6maXPheIVlKGK7IGDBVnAh[2ynYY\h[4U>M3LFSVI{PDl7OEe0
HL-60 NUTxNmtjS3m2b4TvfIlkcXS7IFHzd4F6MlnxNlDPxE4EoB?=NILsR4szPGh?MmPwdoVlfWOnczD0bIUh[3m2b4TvfIlkKGWoZnXjeEBw\iCHcom1MV[yN|Q6PDR6MB?=
HANXPKeGl3SXCxcITvd4l{KEG|c3H5MYW1NO69VQ>?MofBNlRpMmH4d5VxeHKnc4Pld{B1cGViY3zlZZZi\2Vib3[gVGFTWCCjbnSgZ4F{eGG|ZT2zMEAuPyxiYX7kJE06KGmwZIXj[YQh[nliYn;yeIV7d22rYj;TRWhCM17FclI{PDd3OUW2
C666-1NGn5cVRCeG:ydH;zbZMhSXO|YYm=MmrQOVDPxE1?NUHDV3loOjSqNGfBRnF{fXCycnXzd4V{KHSqZTDjcIVifmGpZTDv[kBRSVKSIHHu[EBk[XOyYYPlMVMtKC15LDDhcoQhNTliaX7keYNm\CCkeTDic5J1\XqxbXniM3NCUEF?NV\KVol7OjN2N{W5OVY>
Hepa1-6NVKwVVF5SXCxcITvd4l{KEG|c3H5MYK1NO69VQ>?M{TEcVJpMVfpcohq[mm2czDjeZJkfW2rbjDhcoQhemW|dnXyZZRzd2xvaX7keYNm\CCjcH;weI9{cXN?Mke1NlM1PDZ5NUO=
PLC/PRF/5cM4j2PWFxd3C2b4Ppd{BCe3OjeR?=MWC1NO69VQ>?M{CyUVFpM1\WfpBz\X[nboTzJIFxd3C2b4Ppd{B1emmpZ3Xy[YQh[nliQ1nOMYGyN|Q{QDh{NB?=
HCT116MkmzRZBweHSxc3nzJGF{e2G7M3\PSFExOM7:TR?=NWS4dpE1OS53aB?=NUXTdXk4cW6qaXLpeJMhV3NvaX7keYNm\CClZXzsJIRm[XSqNV7QPIV6OjN5NESzOVM>
HCECM33ae2Z2dmO2aX;uJGF{e2G7M3LU[|Ux|ryPMkXWO|JpMYLEUXNQM2rjU5Jme3SxcnXzJI9nKHSqZTDuc5Ju[WxiSFPFR{BxcGWwb4T5dIU>M1\2elI{PzR{MEGx
Primary rat cerebral cortical neuronsNWDkbnNsSXCxcITvd4l{KEG|c3H5NVnFVI05OTByzszNNYPz[oRyOWh?MX3wdoV3\W62czDD[E1qdmS3Y3XkJIFxd3C2b4Ppd{BidmRiY3XscEBl\WG2aB?=MlXiNlM4PDF|MUe=
MDA-MB-231 MlHURZBweHSxc3nzJGF{e2G7Ml73NlXPxE1?MX6yOIg>M{TTboFjem:pYYTld{BkgXSxdH;4bYNqfHliYX7kJINt\WG4YXflJI9nKGOjc4Dhd4UuOyCjbnSgVGFTWCCrbnT1Z4VlKGK7IGLnNy=>M1n6dFI2OzN5NUS0
NLRP3-Tet-on-MC/9 M1;5dmFxd3C2b4Ppd{BCe3OjeR?=M2POPVExNTRyzszNMmDuNVJpNG\QS3VifHSnboXheIV{KHSqZTDj[YxtKGSnYYToJINifXOnZDDifUBESVCVLXHzd49kcWG2ZXSgUmxTWDNibYX0ZY51eyCrbjD0bIUhXGW2LX;uJJN6e3SnbR?=NI\j[mgzOzdyM{O4PS=>
KNS42 M1W2d2Fxd3C2b4Ppd{BCe3OjeR?=M1rqWVUx|ryPNW\peVB7OWh?M2K5VJJm\HWlZYOgZ4VtdCCmZXH0bEBidmRiY3;tdIxmfGWueTDhZo9tcXOqZXSgZ4F{eGG|ZTCzM|ch[WO2aY\peJkhcW5icnXzdI9ve2VidH:gRWJVNTJ4Mz:ySGcwdWW2Zn;ycYlvKGOxbXLpcoF1cW:wNGC0bVgzOzZ7MUG0OS=>
MCF-7 MlKyRZBweHSxc3nzJGF{e2G7NV3GNWQ5OjEQvF5CpC=>M3vXRVczcA>?NFTUW41qdmirYnn0d{BmeXWxbD2gZY5lKDRvT1jUMY1m\GmjdHXkJIFxd3C2b4Ppdy=>MXKyN|Y4PTZ2Mx?=
hCMEC/D3 NHPGVpNCeG:ydH;zbZMhSXO|YYm=NU\nblE4OjYQvF2=M1no[FczcA>?M13tVZJm\HWlZYOgUJR5SSCrbnT1Z4VlKGGyb4D0c5Nqew>?M2PZWFI{PjZ3MUm4
JurkatMVPBdI9xfG:|aYOgRZN{[Xl?M3vPSlEzNjVvNUFOwG0>NFvzNWIycA>?NGXtdWJld3OnIHTldIVv\GWwdHz5JJN2eHC{ZYPz[ZMhW1CKLXnu[JVk\WRiUHHyMVQh[2ynYY\h[4UtKFCDUmCgZ4xm[X[jZ3WsJGRPSSCocnHncYVvfGG2aX;uMEBidmRibH;zd{Bw\iC4aXHibYxqfHl?NWnkd|lKOjN2NEK5O|Y>
CNE-1NGKzVJpCeG:ydH;zbZMhSXO|YYm=M3LxcVIx|ryPwrC=NXLNeWNsOjSqMoS0bY5pcWKrdIOgVmFFODBzLXnu[JVk\WRiY3XscEBl\WG2aB?=Mnu1NlM1OjZ6NUC=
HONE-1M37tPWFxd3C2b4Ppd{BCe3OjeR?=M1m5UFIx|ryPwrC=M2nvPVI1cA>?NX\4PWJXcW6qaXLpeJMhWkGGMECxMYlv\HWlZXSgZ4VtdCCmZXH0bC=>MV[yN|QzPjh3MB?=
astrocytes cellM2\vd2Fxd3C2b4Ppd{BCe3OjeR?=NGmz[Xg1OM7:TR?=MmjMOog>MX7y[YR2[2W|IHXhdox6KGGyb4D0c5NqeyCrbnT1Z4VlKGK7IIP0ZZVzd3Oyb4LpcoU>MX2yN|QyOTd5OB?=
U-937M1LCSGFxd3C2b4Ppd{BCe3OjeR?=MVOxNO69VQ>?NYTCU21XOzCvaX6=NI\pOoRxemW4ZX70d{BVVkZvaX7keYNm\CCwZXPyc5B1d3Orcx?=MVyyN|QyODd2OB?=
MDA-MB-231M3XFSmFxd3C2b4Ppd{BCe3OjeR?=NIPqVJoyODEQvF2=NGrvbpIycA>?Mne2bY5pcWKrdIOgd4Vve2m2aYrheIlwdiC2bzDUVmFKVCC3cH;uJG1VTEhiZH;3ck1z\We3bHH0bY9vNHK2S2UzOzRyOESyPS=>
HeLa MlnLRZBweHSxc3nzJGF{e2G7MYCxNFDPxE1?NInoPIszcA>?M{nCWYJtd2OtczDKVnMuOTViSX7keYNmeyCDcH;weI91cWNiQ3XscEBF\WG2aB?=NUi4[|ExOjN|NESwOFU>
Ec109NE\Hc2pCeG:ydH;zbZMhSXO|YYm=NFLx[o0yOM7:TR?=NHrsN3M3cA>?MUTicI9kc3NiYYDvdJRwfGmlIHPvcYJqdmG2aX;uJI9nKFSjdD3TcYFkVjdiYX7kJJJi\GmjdHnvci=>NH7RbXczOzN|OEW2PC=>
H460 NUD0fIh{SXCxcITvd4l{KEG|c3H5MmLONVDPxE1?NF7SVVg3cA>?NWHI[lBT[myxY3vzJIFxd3C2b4TpZ{Bkd22kaX7heIlwdiCxZjDUZZQuW22jY163JIFv\CC{YXTpZZRqd25?MljCNlM{Ozh3Nki=
HeLa NHTOR2tCeG:ydH;zbZMhSXO|YYm=NIHVbYg2OM7:TR?=NVrafIt7OS53aB?=NEfKdm9i[nKxZ3H0[ZPDqEOqbHHtfYRq[S2rbnT1Z4VlKGGyb4D0c5Nqew>?MXyyN|MxOzhyNB?=
SK-HEP1MVPBdI9xfG:|aYOgRZN{[Xl?Mm\sNVAx|ryPMXKxbC=>MXrpcohq[mm2czDDdnQyKGGldHn2ZZRm\CClYYPwZZNmNTNuID23MEAuQCxiLUmsJIFv\CCyb3z5LGFFWC2{aXLvd4UqKHCxbInt[ZJie2V?NF3RfW8zOzNyMk[1NC=>
QGY7701MUHBdI9xfG:|aYOgRZN{[Xl?NG\1ZnYzPc7:TR?=Mme3NU42cA>?NHG4NGlqdmirYnn0d{Bi[2O3bYXsZZRqd25ib3[gd5VjNUdzIIDoZZNmKGmwZIXj[YQh[nliRF;YJEsheXWncnPleIlvMl\DNlMzPDByNkG=
HepG2MoruRZBweHSxc3nzJGF{e2G7MY[yNO69VQ>?NUnyOlJpOzCvaX6=NWriO5AzcW6qaXLpeJMhfGinIHXubIFv[2WmIHPlcIwh\GWjdHigZpkh[2:vYnnu[YQhfHKnYYTt[Y51KG:oIHHwbYdmdmmwIHHu[EBVWkGLTB?=Ml\yNlMzOjR{M{m=
U87 NH;ZVGtCeG:ydH;zbZMhSXO|YYm=MV6yOe69VQ>?MWKybC=>MmrF[IVkemWjc3XzJIl{d2yrcYXpdol1cWenbnnuJEhKW0xrLXnu[JVk\WRiYYDvdJRwfGmlIHPlcIwh\GWjdHisJIJ2fCCwb4SgcoVkem:2aXOgZ4VtdCCmZXH0bC=>NFHsR2IzOzJ{OU[yOi=>
HSC-2NX\pbFB2SXCxcITvd4l{KEG|c3H5MmL4NlUwPTEQvF2=MYmxbC=>MXLpcohq[mm2czDTUk0{QC2rbnT1Z4VlKGO7dH;0c5hq[2m2eR?=NHzvU3MzOzF3NUK0PC=>
HSC-4NEHDPVNCeG:ydH;zbZMhSXO|YYm=NET0XVkzPS93MN88US=>NInBbnQycA>?MmG5bY5pcWKrdIOgV24uOzhvaX7keYNm\CCleYTveI95cWOrdIm=MYSyN|E2PTJ2OB?=
CL-1NGXhXZRCeG:ydH;zbZMhSXO|YYm=Moj2NlDPxE1?NHXUfY0ycA>?MoP1Zoxw[2u|IF;QOFQ6KGmwZIXj[YQh[2WubDDk[YF1cA>?NE\NeGgzOzF|MUe4Ni=>
MELM1;6fGFxd3C2b4Ppd{BCe3OjeR?=M{G2cVIx|ryPNHPOPZQzPGh?MlGySG1UVw>?NEjo[HpqdXCjaYLld{BROlh5LXnu[JVk\WRiTVXMJINmdGxiYYDvdJRwe2m|M4W4OlI{ODF2OEi3
Bel-7402 NVjCb4JrS2G|cHHz[UBC[3SrdnH0bY9vKEG|c3H5NHnuU4Y2OM7:TR?=MXOybC=>NEjveGpqdmirYnn0d{BRS0VvaX7keYNm\CCjbn;pb4l{NUPQdWdiOjNyMEi3OFI>
Eca-109NY\OfGp{SXCxcITvd4l{KEG|c3H5MXOyOe69VQ>?MYqzNI1qdg>?MV3pcohq[mm2czDCTk1DOTFvaX7keYNm\CClZXzsJIRm[XSqNVvjfXJGOjNyN{[5Olc>
MELNEfzWZhCeG:ydH;zbZMhSXO|YYm=Mof0NlDPxE1?NGXkeZoycA>?NIfnNYhFVVORNGT2e2ZqdXCjaYLld{BROlh5LXnu[JVk\WRiTVXMJINmdGxiYYDvdJRwe2m|M4\1WVI{ODF2OEi3
Bel-7402 NWS0SnhGSXCxcITvd4l{KEG|c3H5NX;kV|k6PTEQvF2=Ml7iNog>M{\DZYlvcGmkaYTzJHBETS2rbnT1Z4VlKGGwb3nrbZM>NHmzd3UzOzByOEe0Ni=>
L929NHrJPGhHfW6ldHnvckBCe3OjeR?=M2PIU|IvPc7:TR?=MonvNYg>NX7JZpdCcW6lcnXhd4V{KFKLUEGg[ZhxemW|c3nvckBidmRiZYjhZ4Vz[mG2ZXSgWG5H|rFvaX7keYNm\CCvaYTvZ4hwdmS{aXHsJIR6e2[3bnP0bY9vKGGwZDDSU3MheHKxZIXjeIlwdg>?MlLrNlMxODB3MUi=
RCC NInEWmNE\WyuIG\pZYJqdGm2eTDBd5NigQ>?NHn4TWwyODEQvF2=M3G4e|I1cA>?Ml;YdoVkd3[ncnXzJJRp\SC4aXHibYxqfHlib3[gZ4VtdHNiZYjwc5Nm\CC2bzCxOYQuWEeMMh?=MVGyNlk6OTR7NB?=
NB2a/d1MUnBdI9xfG:|aYOgRZN{[Xl?M3PMPVExOM7:TR?=NITXO2I4Omh?NH;CRVJifHSnboXheIV{KHO2YYXyc5Nxd3KrbnWtbY5lfWOnZDDjZZNx[XOnIHHjeIl3cXS7LDDQRXJRNCCjbnSgeIF2KGOuZXH2ZYdmMYiyNlk5QDV2MR?=
T cellMVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MmC4NlUuOTByzszNNYWzVYx6OjSqMl;5[I9{\S2mZYDlcoRmdnSueTDpcohq[mm2ZXSgWEBk\WyuIIDyc4xq\mW{YYTpc44hdWWmaXH0[YQhfGi{b4XnbEB1cGViY3:td5RqdXWuYYTpc44hf2m2aDDhcpRqNUOGMzDhcoQh[W62aT3DSFI5NX;vR2IzOjJ7OEK1N|g>
K562M1XVbmFxd3C2b4Ppd{BCe3OjeR?=MVKxNFDPxE1?MUGxbC=>NXHqSpBt[myxY3vzJGFjdm:kYY\pd4N2dcLiRj3pcoR2[2WmIHHwc5B1d3Orcx?=MWSyNlk4OjN5Mh?=
Jurkat MXXBdI9xfG:|aYOgRZN{[Xl?MoXQOFDPxE1?M13Qe|FpM3XnZYFjd2yrc3jld{BH[XOOLXnu[JVk\WRiY3HzdIF{\SCjY4TpeoF1cW:wIHHu[EBk\WyuIHTlZZRpNV3GXId1OjJ7NEK3N|g>
BGC-823NYfDe5ZYSXCxcITvd4l{KEG|c3H5M{HHNVExOM7:TR?=MmjaNYg>MYXwZZJ1cWGubImgdoV{[3WnczDj[YxteyCjZ3HpcpN1KGSjbXHn[UBw\iCmYXnkfoVqdg>?M{HNeVIzQTJ4NUS1
Hep3BNETaRlJCeG:ydH;zbZMhSXO|YYm=MUm1NO69VQ>?NUTRbpNWOWh?MWDicI9kc3NiYYDvdJRwe2m|IHnu[JVk\WRiYomgTGVIS3N?NI\h[IszOjl{M{G1OC=>
LLC-PK1M1;WTWFxd3C2b4Ppd{BCe3OjeR?=MY[yNO69VQ>?NF\uZVkycA>?MlHmdJJmfmWwdIOgZ4l{eGyjdHnuMYlv\HWlZXSg[IVoemGmYYTpc44hd2ZiQYTnOUwh[mWlbHnuMVEtKGGwZDDBeIcyOiCycn;0[Ylvew>?MnL1NlI5QTZyM{e=
A549M13EfWFxd3C2b4Ppd{BCe3OjeR?=Ml\lOVDPxE1?MnHSNYg>NFXzWVljdG:la4OgeIhmKEKDST3pcoR2[2WmIHHwc5B1d3Orc9MgM1S0VlIzQDh5MkG1
SGC-7901 M2[0eWFxd3C2b4Ppd{BCe3OjeR?=NXrkW4FVOTEQvF2=Mki2NlRpMWPpcohq[mm2czFOtkzPui2maX3leIh6dGGlconsd4hqc2:waX6tbY5lfWOnZDDhdI9xfG:|aYRCpC=>MYOyNlg1QDV7Nx?=
DM6M1;o[mFxd3C2b4Ppd{BCe3OjeR?=MYexNFDPxE1?NYLjXW1{PzKqMYPicI9kc3NiYn;0bEBGOkZvMTDhcoQhTTKIdIKtcYVlcWG2ZXSgZ5l1d3SxeHnjbZR6M{e3eVIzQDJ3M{K4
MCF-7, MDA-MB-468, Caco-2NYLWRWN7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MXi1NO69VQ>?NWf4R29RPDiqMVTpcohq[mm2czD0bIUh[2WubDDndo94fGhiaX7obYJqfGmxbjDpcoR2[2WmIHL5JJNieG:waX6=NFrTU2czOjhyMEm2PC=>
A2750 NEfxXGFCeG:ydH;zbZMhSXO|YYm=MV[yNO69VQ>?MUSybC=>NF;sTIlFVVORNEP5fGljdG:la4OgZ4F{eGG|ZTDjcIVifmGpZTDkeZJqdmdiaHXs[Y5idGmwIITy[YF1dWWwdDDhcoQhemWmdXPld{BifXSxcHjh[4lkKGOnbHyg[IVifGh?MUmyNlc5PDN4Mx?=
U87 MX3BdI9xfG:|aYOgRZN{[Xl?NVvWdJhSOjEQvF2=MYWyOIg>MlHodoVlfWOnczD0bIUh[XCxcITvd4l{KHKjdHWgbY5lfWOnZDDifUBRVEGENWfid|ZwOjJ5N{i3PFA>
HT1080NIG2R2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NVnmZmw2PTEQvF2=Mn7hO4Q>NYnQVnU1cW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJIlvcGmkaYTpc44h[2G3c3XkJIJ6KGOxbXLpcoVlKHS{ZXH0cYVvfCCxZjDER2Eh[W6mIF;NVC=>MlP3NlI4PDB7OES=
A549 NVn3NG4ySXCxcITvd4l{KEG|c3H5NVf4TFYyPTEQvF2=M2D5ZVJpM4Dud5BienSrYXzsfUBl\WO{ZXHz[ZMhe2:maYXtJJNmdGWwaYTlMYlv\HWlZXSgZZBweHSxc3nzNYfnc4I4OjJ5MkG4NFQ>
Primary OPCMn7zRZBweHSxc3nzJGF{e2G7NGjJSYYy|ryPM3TiPFZpNzJ2aB?=NGXMNINz\WS3Y3XzJJRp\SCyZYLj[Y51[WenIH;mJINmdGy|IHnuJIViemy7LTDhcoQhdGG2ZT3hdI9xfG:|aYOvcoVkem:|aYO=M{nrbVIzPzB5M{i1
PMNsMnXWRZBweHSxc3nzJGF{e2G7M2jiVFQx|ryPMXK2bC=>MlrGSG1UVw>?NGnvVZBz\X[ncoPl[EB1cGViYX3veY51KG:oIHPs[YF3\WRiY3HzdIF{\S1|IITvJI5m[XJidnXobYNt\SCuZY\lcJM>MkP4NlI3QTJ3N{e=
A549 NF;QfFlCeG:ydH;zbZMhSXO|YYm=MX61NO69VQ>?NXWzWHJYOWh?M3roWZBz\X[nboTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEKjb3j1c5Nq\GViSR?=MXqyNlY5PzZ|NR?=
AGSNXjQ[242SXCxcITvd4l{KEG|c3H5MYCyNO69VQ>?M1e3WlEzcA>?NHfNfYdqdmirYnn0d{B1cGViYXP0bZZifGmxbjDv[kBxem9vY3HzdIF{\S1|IHnuJJJme3CxboPlJJRwKHSqZTDFeG9C[yCocnHjeIlwdg>?MYCyNlY5PzN7OB?=
shC9 NXPzOmdOSXCxcITvd4l{KEG|c3H5M1;scVEx|ryPMorqNVZpNV\2Z2JY[my3boTzJHNJUCCneIDy[ZN{cW:wIHnuJJNpSzliY3XscJMh\Xiyb4Pl[EB1dyCnaYTo[ZIhWEFib4KgUHBENF7IR2MzOjZ2MUC5OC=>
primary MEFsM3jzTWZ2dmO2aX;uJGF{e2G7MYmxNog>MX9CpIlv[3KnYYPld{BmfG:yb4Pp[IUucW6mdXPl[EBucXSxY3jvcoRzcWGuIH3lcYJz[W6nIHTldI9t[XKrenH0bY9vM{DobVIzPjF|N{[3
3T9 MEFsNW\JUo5ETnWwY4Tpc44hSXO|YYm=MXixOog>NFu4UIVqdmO{ZXHz[ZMh[3m2b3Podo9u\SClIILlcIVie2ViYX\0[ZIh\XSxcH;zbYRmNXS{ZXH0cYVvfA>?MXWyNlYyOzd4Nx?=
3T9 MEFsM130[2Z2dmO2aX;uJGF{e2G7NUXE[VhHOTiqMUX1dJJm\3WuYYTld{Bqdmm2aXH0c5Ih[2G|cHHz[U06KGK3dDDkc5dvemWpdXzheIV{KGWoZnXjeI9zKGOjc4Dhd4V{KGGodHXyJIV1d3Cxc3nk[UB1emWjdH3lcpQ>NXTONmVlOjJ4MUO3Olc>
MDA-MB-231 NIDaZZRCeG:ydH;zbZMhSXO|YYm=NIqzOXMzOM7:TR?=MnfPOIQ>MmDTdoVlfWOnczD0bIUh[2WubDDhdI9xfG:|aYOgbY5lfWOnZDDifUBu\XSob4LtbY4hfHKnYYTt[Y51KHejczDzbYdvcW[rY3HueIx6NXXTVFF[OjJ3OUO0OFE>
C6 NXrrZ45MSXCxcITvd4l{KEG|c3H5MonMNVDPxE1?Mk[wNlRpMnLDZoxw[2u|IITo[UB{fXCycnXzd4l3\SCnZn\lZ5Qhd2ZidHjlJJBmeHSrZHWgc44hfmmjYnnsbZR6M1jpdlIzPTh6OUiw
HL-60 NVrvbIdISXCxcITvd4l{KEG|c3H5M4nWU|ExOM7:TR?=MkPsNlRpMnP0bY5pcWKrdIOgeIhmKGOnbHygZZBweHSxc3nzJJNmdGWldHXkJEgsMS2vZX70bJltKM7{LTix5qaTPilvZHnncJVkd3C7cnHuc5Nq\GYEoEW=M3v0fFIzPTR4Nk[5
HL-60Ml;QRZBweHSxc3nzJGF{e2G7NUnJS|VCOTBvOEFOwG0>NFrEc|M1cA>?NUm0RXBucW6qaXLpeJMhXEeKUT3pcoR2[2WmIHPlcIwh[XCxcITvd4l{yqB?MVSyNlUzOzJ{OR?=
BCCNXLEOpJWSXCxcITvd4l{KEG|c3H5NFfwTmo2OM7:TR?=NG\xWnMycA>?NV\GUHFqcW6qaXLpeJMhTEGWUz3t[YRq[XSnZDDndo94fGhiaX7obYJqfGmxbh?=NYPwOllOOjJ3MUm0N|Y>
RAW 264.7NIjhOYVCeG:ydH;zbZMhSXO|YYm=NITVTGc2OC9zMEFOwG0>MUmxbC=>NHiwdlVkd26lZX70doF1cW:wLXTldIVv\GWwdDDpcohq[mm2czDEU24ucW6mdXPl[EBzWk6DIHPs[YF3[WenM2Ph[VIzPDlzNEK2
K562NU\lTYtkSXCxcITvd4l{KEG|c3H5MWCyOe69VQ>?M336d|JpM3XnPZBz\X[nboTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KGOxLYTy[YF1dWWwdDD3bZRpKGGvdYLlcpNqdiCJIHHu[EBVWkGLTB?=MmT6NlI1QDN5N{e=
SGC-7901M{TRO2Fxd3C2b4Ppd{BCe3OjeR?=MoC4NlAh|ryPwrC=NX\zOlNmOmh?M3LseIF1fGWwdXH0[ZMhUDKRMtMgc5IhXE6IIN8xMYlv\HWlZXSgZ4VtdCCjcH;weI9{cXNiYYOge4VtdCCjczDjZZNx[XOnLUOgZYN1cX[rdIpCpC=>MorQNlI1PzF3OEm=
PC3MkjuRZBweHSxc3nzJGF{e2G7MUSxNO69VQ>?MmTNOIg>NUjFVJI5[2:3boTldpMh\myjdn;jc5hq\C2rbnT1Z4VlKGOjc4Dhd4UuemWuYYTl[EBieG:ydH;zbZM>MonqNlI1PzF7N{S=
SMMC-7721NWjHR4FZSXCxcITvd4l{KEG|c3H5NEfTSnY2OM7:TR?=NYXEfZl{PDiqMYXheJRmdnWjdHXzJI9VWi2rbnT1Z4VlKGGyb4D0c5Nqew>?NWLV[mNZOjJ2NkW4N|M>
HeLa MnzsRZBweHSxc3nzJGF{e2G7MUm1NOKh|ryPM3\VUVQwQGh?MXnpcohq[mm2czDTWHMucW6mdXPl[EBt[XSnLYDoZZNmKGGyb4D0c5Rq[yCndnXueJM>MWCyNlQ3ODVyNB?=
HeLa M1rVPGFxd3C2b4Ppd{BCe3OjeR?=M4POXFUxyqEQvF2=MX2xbC=>M1TaV5N2eHC{ZYPz[ZMhfGinIF\SRXAucW6mdXPl[EBi[2O3bYXsZZRqd25ib3[gZY5v\XirbjDWJJBwe2m2aY\lJINmdGy|MoDmNlI1PDl2NEC=
T47D MXzBdI9xfG:|aYOgRZN{[Xl?M3HLclExOM7:TR?=MVixbC=>NH\ySW9jdG:la4OgeIhmKGenbnXyZZRqd25ib3[gSU1k[WRxQ2TGNkBjgSCVVGO=NF\mV3QzOjRyMUG2PC=>
HeLa M2rPc2Fxd3C2b4Ppd{BCe3OjeR?=NXvycnlUOzEQvF2=MWq0bC=>NVzGUnhvyqCrbnPy[YF{\XNidHjlJIdmdmW{YXygZ4VtdCC4aXHibYxqfHliNEigbEBi\nSncjDwbI91d2S7bnHtbYMhfHKnYYTt[Y51yqB?M{jKblIzOzl2MkS4
HCCMVvBdI9xfG:|aYOgRZN{[Xl?MnSzNlAh|ryPwrC=MmflNog>NV:wWHBW[XS2ZX71ZZRmeyC2aHWgSGhCKGmwZIXj[YQh[WO2aY\heIlwdiCxZjDQRXJRM1O5W|IzOzR{N{Oy
mESCsMlHFRZBweHSxc3nzJGF{e2G7MVKyMlXPxE1?NV[wWItUOmh?NHjDWG5qdmirYnn0d{B1cGViTnHGMY1m\GmjdHXkJINie3Cjc3WgZYN1cX[jdHnvci=>MlPNNlIzQDV{N{S=
EMT-6 MlnLR5l1d3SxeHnjbZR6KEG|c3H5MUKxNFDPxE1?MV[xbC=>NF3KbINx[XK2aXHscJkh[myxY3vl[EBk\WyuIHTlZZRpKGmwZIXj[YQh[nlic3nyZY1me2mwZR?=NE[zR|IzOjJ3MUmyNS=>
MCF7Mnf4RZBweHSxc3nzJGF{e2G7NFPNPYI2OMLizszNMoO4NYg>NXHlZW5DcW6qaXLpeJMhWEFiYXP0bZZifGWmIHPhd5Bie2VvMzygMVktKGGwZDDwc4x6MEGGUD3ybYJwe2VrIIDvcJlu\XKjc3W=NGPR[lgzOjJ{M{O0OS=>
K562M{LTS2Fxd3C2b4Ppd{BCe3OjeR?=NXznd5drOjBizszNxsA>M2\0flQ5cA>?M{LUOWROW09?NUXqelJ4[myxY3vzJIxieGG2aX7pZk1qdmS3Y3XkJIlvcGmkaYTpc44hd2ZidnnhZoltcXS7IHHu[EBieG:ydH;zbZMhcW6mdXP0bY9vNXjRV2d{OjJ{MU[xOVg>
Molt4-hygNUTZOoJbSXCxcITvd4l{KEG|c3H5NXi2dJNwOTEQvF2=M3TPd|AvPWh?MXLicI9kc3NiZnHycoV{d2xvaX7keYNm\CClYYPwZZNmNTNvbHnr[UBi[3Srdnn0fS=>MmnwNlYzPzV6MUG=
HeLaNWnEe5FsSXCxcITvd4l{KEG|c3H5MWCxNO69VQ>?NHfufpExNjWqMo\6bY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkhfGinIHPvcYJqdmWmIITy[YF1dWWwdDD3bZRpKGexbXnzbY4hViCjbnSgWHJCUUx?NGfOV4wzOjF5OU[2NS=>
Jurkat TMX;BdI9xfG:|aYOgRZN{[Xl?M1K1dlMx|ryPMmjqNE42cA>?NFu3Z2dFVVORMoPnZoxw[2u|IITo[UB7cXKjbT3pcoR2[2WmIHHwc5B1d3Orcx?=MW[yNlE2QTh7OB?=
NeutrophilNGTCRWhCeG:ydH;zbZMhSXO|YYm=MUeyNO69VQ>?M3PTUlAvPWh?MnX0ZZR1\W63YYTld{B1cGVicILvMYFxd3C2b4TpZ{Bm\m[nY4Sgc4YhVWGUMR?=MmHPNlYyQTZ6NES=
HCT116M4TafGFxd3C2b4Ppd{BCe3OjeR?=MlTzOVDDqM7:TR?=MVuybC=>NYDxe3FNemW4ZYLz[ZMhe3mwZYLnbZN1cWNiYYDvdJRwe2m|IHXm[oVkfHNib3[gZ4Vt\WOxeHniJIFv\CCQUFOtNVbDqA>?NXTldYRWOjJzNUm3OVI>
MDA-MB-231 M4TVNGFxd3C2b4Ppd{BCe3OjeR?=Ml\hNk42NTdwNd88US=>M1;uS|JpNYHHUXdEcW6qaXLpeJMhfGinIHPlcIwh\GWjdHigc4YhVUSDLV3CMVI{OSClZXzsd{BqdmS3Y3XkJIJ6KFOGVDDpckBiKGOxbnPlcpRz[XSrb36g[IVx\W6mZX70JI1idm6nch?=NITLN3IzOjFzNUWyOi=>
LNCaPMl7PRZBweHSxc3nzJGF{e2G7M1jnUVQx|ryPM3zuVlJpMkTIbY5pcWKrdIOgZpV1\WmwIHnu[JVk\WRiY3XscEBieG:ydH;zbZM>NIfKVoIzOjFzNEe2OC=>
MB231Mo\oRZBweHSxc3nzJGF{e2G7MlPoNVAx|ryPM3P5fVFpNX\qSFZFTE2VTx?=NELjSlZi[nKxZ3H0[ZMhfGinIHnu[JVkfGmxbjDv[kBk\WyuIHTlZZRpKGK7IGfFSVEhcW6qaXLpeIlwdixiVGLBTWwhfHKnYYTt[Y51NCCjbnSgeIhmKGOxbXLpcoF1cW:wNYDtNlRFOjJzMUK5OFA>
HCC38 NFTjenBCeG:ydH;zbZMhSXO|YYm=NVe4e2pyOTByzszNNHezS5oycA>?NWi2[W5STE2VTx?=M{nPPIFjem:pYYTld{B1cGViaX7keYN1cW:wIH;mJINmdGxiZHXheIgh[nliV1XFNUBqdmirYnn0bY9vNCCWUlHJUEB1emWjdH3lcpQtKGGwZDD0bIUh[2:vYnnuZZRqd25?NGrEVHQzOjFzMkm0NC=>
MDA-MB231NGrkTHVCeG:ydH;zbZMhSXO|YYm=NYe3T5hYPTEEoN88US=>M3TENFI1cA>?NILDVVVFVVORNIfwcXZi[nKxZ3H0[ZMh[3W{Y4XtbY4ucW6mdXPl[EBk\WyuIHTlZZRpNW\sSGIyOjJzMEGzN|U>
LNCaPMlvHRZBweHSxc3nzJGF{e2G7MWK1NOKh|ryPM4\PS|I1cA>?M{S3b2ROW09?NYjYeHh1[WK{b3fheIV{KGO3cnP1cYlvNWmwZIXj[YQh[2WubDDk[YF1cA>?NH3sTXkzOjFyMUOzOS=>
HCT116MX\BdI9xfG:|aYOgRZN{[Xl?NFjPV4U2OMLizszNMoT2NlRpNFzVXXZFVVORMkniZYJzd2ejdHXzJIN2emO3bXnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=>NH\wVFczOjFyMUOzOS=>
Ishikawa MXfBdI9xfG:|aYOgRZN{[Xl?MlfVNlXPxE1?NFToRowzPGh?NHf0V2Rz\WS3Y3XzJINmdGxiZHXheIgh[W6mIHHwc5B1d3OrczDpcoR2[2WmIHL5JGJi\iCDMR?=NV7jc|RGOjJyOEi5NVg>
 YD-8 MYDBdI9xfG:|aYOgRZN{[Xl?M{nGdlExOM7:TR?=MUGxbC=>NXXZb4U2cW6qaXLpeJMhWEyHTz3pcoR2[2WmIHHwc5B1d3Orcx?=MnP5NlIxQDZzOEO=
eosinophil MVjBdI9xfG:|aYOgRZN{[Xl?MUW5NO69VQ>?NV7lb5pvOjSqNGXETpRqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSCIYYOgZY51cWKxZIpCpC=>NYDmfpVJOjJyN{mzN|Q>
L929NWDMXIRIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NU\pNYVIOi53zszNMlTYNlRpM2K5cIlvcGmkaYTzJJRp\SCneIDy[ZN{cW:wIH;mJJAueDN6IHHu[EBPTi4QulKgeI8h[XWpbXXueEBVVkcQsT3pcoR2[2WmIH7lZ5JweHSxc3nzJIFv\CCjdYTvdIhi\3l?MXuyNlAzPzB7Nx?=
YD-8 NVn2cplHSXCxcITvd4l{KEG|c3H5M2jGOlExOM7:TR?=NUPMWlBMOWh?M4TncoJtd2OtczD0bIUhT1NvSFPsMYlv\HWlZXSgZZBweHSxc3nzMV:yNlAzODB5OB?=
HBxNG\pU2dCeG:ydH;zbZMhSXO|YYm=MnXJNlXPxE1?MXK0PIg>M4W3WGROW09?M4G2V5Jm\HWlZXSgZ4VtdCCmZXH0bEBqdmS3Y3XkJIJ6KDNvTVG=NH\2RWQzOjB{MEC3PC=>
U937 NHXJc2lCeG:ydH;zbZMhSXO|YYm=Mm[yOVDDqM7:TR?=MlO4NYg>NVnRSGlscW6qaXLpeJMhUEZvaX7keYNm\CCjcH;weI9{cXQEoB?=NXnwdpk3OjF7OUi3N|E>
HL60MULBdI9xfG:|aYOgRZN{[Xl?M2TKeVQx|ryPNGDOfVQ1OG2rbh?=NHrmRYVFVVORM1ntSIJtd2OtczDCUmREKGOxbYDveY5leyCrbnT1Z4Uh\Xiyb4P1doUhd2ZicHjvd5Bp[XSrZInsd4VzcW6nIHHu[EBFVkFiZoLh[41mdnSjdHnvci=>M{fSbVIyQTh|Mkm2
M-14M{fhUGFxd3C2b4Ppd{BCe3OjeR?=MkfFNlXPxE1?M3nPV|AvPWh?MVdCpIlvcGmkaYTzJIJwfGhidHjlJINzfWSnIHX4eJJi[3RvIHHu[EBkd22yb4Xu[E1qdmS3Y3XkJIFxd3C2b4Ppdy=>MXqyNVk2PDl3OR?=
SK-BR-3Mo\rRZBweHSxc3nzJGF{e2G7MYC1NOKh|ryPNVz0WIhHOmh?NIT6VFNjdG:la4OgZZBweHSxdHnjJGRPSSCocnHncYVvfGG2aX;uJIlv\HWlZXSgZpkh[2:vYnnuZZRqd25ib3[gV20uOTZ2IHHu[EBz[WSrYYTpc44>MYiyNVkxOTN6Nh?=
MDA-MB-468Ml7vRZBweHSxc3nzJGF{e2G7NIraU4U2OMLizszNM4H0W|JpMlnpZoxw[2u|IHHwc5B1d3SrYzDEUmEh\nKjZ33lcpRifGmxbjDpcoR2[2WmIHL5JINwdWKrbnH0bY9vKG:oIGPNMVE3PCCjbnSgdoFlcWG2aX;uM1fiNVIyQTBzM{i2

... Click to View More Cell Line Experimental Data

In vivo
Features A key compound for apoptosis studies.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDog
Weight (kg)0.020.151.80.40.0810
Body Surface Area (m2)0.0070.0250.150.050.020.5
Km factor36128520
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)
1

References

[1] Slee EA, et al. Biochem J, 1996, 315 (Pt 1), 21-24.

[2] Shimizu T, et al. Leukemia, 1997, 11(8), 1238-1244.

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Chemical Information

Download Z-VAD-FMK SDF
Molecular Weight (MW) 467.49
Formula

C22H30FN3O7

CAS No. 187389-52-2
Storage 3 years -20℃powder
6 months-80℃in solvent
Synonyms N/A
Solubility (25°C) * In vitro DMSO 93 mg/mL (198 mM)
Water <1 mg/mL (<1 mM)
Ethanol 24 mg/mL warming (51 mM)
DMF 84 mg/mL
* Z-VAD-FMK will racemize in DMSO, water and ethanol.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name L-Alaninamide, N-[(phenylmethoxy)carbonyl]-L-valyl-N-[(1S)-3-fluoro-1-(2-methoxy-2-oxoethyl)-2-oxopropyl]-

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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