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Technical Data
| Formula | C15H11F2N3 |
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| Molecular Weight | 271.26 | CAS No. | 1262888-28-7 | |
| Solubility (25°C)* | In vitro | DMSO | 54 mg/mL (199.07 mM) | |
| Ethanol | 54 mg/mL (199.07 mM) | |||
| Water | Insoluble | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Biological Activity
| Description | Spautin-1 is a potent and specific autophagy inhibitor, and inhibits the deubiquitinating activity of USP10 and USP13 with IC50 of ∼0.6-0.7 μM. Spautin-1 enhances apoptosis. | ||||
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| In vitro | In Bcap-37 cells, Spautin-1 dramatically enhanced cell death in glucose-free media and induces apoptotic morphology. In Bax-Bak DKO cells, spautin-1 inhibits etoposide induced autophagic cell death. Spautin-1 promots the degradation of Vps34 complexes by regulating the deubiquitination activity of USP10 and USP13, and reduces the levels of PtdIns3P. [1] In PDGF-treated cells, spautin-1 stabilizes α-smooth muscle cell actin and calponin, prevents actin filament disorganization, diminishes production of extracellular matrix, and abrogates VSMC hyperproliferation and migration. [2] In CML cells, spautin-1 markedly inhibits IM-induced autophagy by downregulating Beclin-1, and enhances IM-induced apoptosis by inactivating PI3K/AKT and activating downstream GSK3β. [3] Spautin-1 also specifically reduces infectious dengue virus titers in BHK-21 cells. [4] |
Protocol (from reference)
References
Customer Product Validation
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Data from [Data independently produced by , , Cancer Lett, 2018, 425:1-12]
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Data from [Data independently produced by , , J Hematol Oncol, 2016, 9(1):45]
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Data from [Data independently produced by , , Int J Cancer, 2015, 136(11):2717-29. ]
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Data from [Data independently produced by , , Toxicol Lett, 2017, 280:206-212]
Selleck's Spautin-1 has been cited by 43 publications
| HSPA12A acts as a scaffolding protein to inhibit cardiac fibroblast activation and cardiac fibrosis [ J Adv Res, 2024, S2090-1232(24)00025-0] | PubMed: 38219869 |
| The critical role of RAGE in severe influenza infection: A target for control of inflammatory response in the disease [ Clin Immunol, 2024, 262:110178] | PubMed: 38460892 |
| USP13 drives lung squamous cell carcinoma by switching lung club cell lineage plasticity [ Mol Cancer, 2023, 22(1):204] | PubMed: 38093367 |
| TRPV1 inhibition overcomes cisplatin resistance by blocking autophagy-mediated hyperactivation of EGFR signaling pathway [ Nat Commun, 2023, 14(1):2691] | PubMed: 37165076 |
| Compensatory cross-talk between autophagy and glycolysis regulates senescence and stemness in heterogeneous glioblastoma tumor subpopulations [ Acta Neuropathol Commun, 2023, 11(1):110] | PubMed: 37420311 |
| Upregulation of the EGFR/MEK1/MAPK1/2 signaling axis as a mechanism of resistance to antiestrogen‑induced BimEL dependent apoptosis in ER+ breast cancer cells [ Int J Oncol, 2023, 62(2)20] | PubMed: 36524361 |
| RTN4/Nogo-A-S1PR2 negatively regulates angiogenesis and secondary neural repair through enhancing vascular autophagy in the thalamus after cerebral cortical infarction [ Autophagy, 2022, 1-20] | PubMed: 35263212 |
| Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy [ Mater Today Bio, 2022, 16:100344] | PubMed: 35833197 |
| USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model [ Oncogene, 2022, 41(13):1974-1985] | PubMed: 35173307 |
| USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model [ Oncogene, 2022, 41(13):1974-1985] | PubMed: 35173307 |
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.