N6-methyladenosine (m6A)

Catalog No.S3190 Synonyms: NSC-29409, 6-Methyladenosine, N-Methyladenosine

For research use only.

N6-methyladenosine (m6A, NSC-29409, 6-Methyladenosine, N-Methyladenosine) is a base modified analog of adenosine and is found as a minor nucleoside in natural RNAs.

N6-methyladenosine (m6A) Chemical Structure

CAS No. 1867-73-8

Selleck's N6-methyladenosine (m6A) has been cited by 12 Publications

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Biological Activity

Description N6-methyladenosine (m6A, NSC-29409, 6-Methyladenosine, N-Methyladenosine) is a base modified analog of adenosine and is found as a minor nucleoside in natural RNAs.
In vitro

N6-Methyladenosine is an abundant modification in mRNA and is found within some viruses, and most eukaryotes including mammals, insects, plants and yeast. It is also found in tRNA, rRNA, and small nuclear RNA(snRNA) as well as several long non-coding RNA, such as Xist. [1] [2] [3] N6-Methyladenosine is an endogenous urinary nucleoside product of the degradation of transfer ribonucleic acid (tRNA). [4] N6-Methyladenosine is a widespread RNA modification in many tissues with high levels in the brain. N6-Methyladenosine is enriched near stop codons and within 3’UTRs in both mouse and human mRNAs. [5] The recent discovery that FTO, an obesity risk gene, encodes an m6A demethylase implicates m6A as an important regulator of physiological processes. [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CHO cells M2SzfGZ2dmO2aX;uJIF{e2G7 NFjW[JlKdmirYnn0bY9vKG:oIGuxNlVKZS2DQj3NSWNCKGKrbnTpcochfG9iaIXtZY4hSWSnbn;zbY5mKEF|IILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMtKEurPUmuN{BvVQ>? NWTG[3V[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3O|E1OjFpPkG1O|cyPDJzPD;hQi=>
CHO cells Mlq5SpVv[3Srb36gZZN{[Xl? MXTJcohq[mm2aX;uJI9nKFt|SG3SMXBKSSCkaX7kbY5oKHSxIHj1cYFvKEGmZX7vd4lv\SCDMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{NCCNaU22JO69VQ>? NWG2Nnd5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3O|E1OjFpPkG1O|cyPDJzPD;hQi=>
CHO cells MlnSSpVv[3Srb36gZZN{[Xl? NEnCcGpKdmirYnn0bY9vKG:oIGuxNlVKZUGELV3FR2Eh[mmwZHnu[{B1dyC{YYSgRYRmdm:|aX7lJGE{KHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8h[2WubIOsJGtqRTZwNDFOwG0> MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd5MUSyNUc,OTV5N{G0NlE9N2F-
adipocytes, heart and brain cells NXPDWVZCTnWwY4Tpc44h[XO|YYm= M12zV2Fn\mmwaYT5JINwdnO2YX70JIZweiCrbnjpZol1cW:wIH;mJGEyKHKnY3XweI9zKGOxboTyc4whd2ZiYXTlcplt[XSnIHP5Z4xie2ViaX6gZYRqeG:leYTld{whcGWjcoSgZY5lKGK{YXnuJINmdGy|LDDB[oZqdmm2eTDjc45{fGGwdE2wMlHPxE1? NU[3fnRGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxNkK3PVg1OCd-NkK3PVg1ODxxYU6=
In vivo LD50: Mice >1g/kg (i.g.). [7]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 281.27
Formula

C11H15N5O4

CAS No. 1867-73-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CNC1=C2C(=NC=N1)N(C=N2)C3C(C(C(O3)CO)O)O

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04696094 Recruiting -- Moyamoya Disease Beijing Tiantan Hospital January 1 2021 --

(data from https://clinicaltrials.gov, updated on 2022-01-17)

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