Home JAK/STAT JAK inhibitor - STAT inhibitor Cucurbitacin I For research use only.

Cucurbitacin I

Synonyms: Elatericin B, JSI 124, NSC 521777

Cucurbitacin-I (Elatericin B, JSI 124, NSC 521777), a natural cell-permeable triterpenoid isolated from Cucurbitaceae, is a novel selective inhibitor of JAK2/STAT3.

Cucurbitacin I Chemical Structure

Cucurbitacin I Chemical Structure

CAS: 2222-07-3

Purity & Quality Control

Batch: S815201 DMSO] 100 mg/mL] false] Ethanol] 100 mg/mL] false] Water] Insoluble] false Purity: 99.19%
99.19

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Signaling Pathway

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JAK Signaling Pathway

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Biological Activity

Description

Cucurbitacin-I (Elatericin B, JSI 124, NSC 521777), a natural cell-permeable triterpenoid isolated from Cucurbitaceae, is a novel selective inhibitor of JAK2/STAT3.
Targets
JAK2 [1] STAT3 [1]
In vitro
In vitro

Cucurbitacin-I (Elatericin B, JSI 124, NSC 521777) can efficiently inhibit the proliferation of GBM cells by blocking phosphorylation of STAT3, which in turn lead to the downregulation of antiapoptotic gene products and promote apoptosis. In addition, JSI-124 induced G2/M phase arrest in GBM via downregulation of cyclin B1 and cdc2 expression.<sup><a class="sref" href="#s_ref">[1]</a></sup> <sup><a class="sref" href="#s_ref">[2]</a></sup><sup><a class="sref" href="#s_ref">[3]</a></sup>
Cell Research Cell lines U251 and A172 cells
Concentrations 12.5, 25, 50, 100, 200, 400 and 1000 nM
Incubation Time 24, 48 and 72 h
Method

Cucurbitacin I (JSI-124) induces proliferation inhibition and morphological transformation of GBM Cells. U251 cells (5 x 103/100 μl) and A127 cells(5 x 103/100 μl) were seeded in 96-well plates in triplicate, incubated overnight, treated with different concentrations of JSI-124 for 24, 48 and 72 h, then subjected to CCK-8 assay at various times to analyze proliferation of cells. Cucurbitacin I (JSI-124) dose-and time-dependently inhibited the proliferation of U251 and A172 cells.

Chemical Information & Solubility

Molecular Weight 514.65 Formula

C30H42O7
CAS No. 2222-07-3 SDF --
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (194.3 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 100 mg/mL

Water : Insoluble


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