Cucurbitacin I

Catalog No.S8152 Synonyms: Elatericin B, JSI 124, NSC 521777

For research use only.

Cucurbitacin-I (Elatericin B, JSI 124, NSC 521777), a natural cell-permeable triterpenoid isolated from Cucurbitaceae, is a novel selective inhibitor of JAK2/STAT3.

Cucurbitacin I Chemical Structure

CAS No. 2222-07-3

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Biological Activity

Description

Cucurbitacin-I (Elatericin B, JSI 124, NSC 521777), a natural cell-permeable triterpenoid isolated from Cucurbitaceae, is a novel selective inhibitor of JAK2/STAT3.

Targets
JAK2 [1] STAT3 [1]
In vitro

Cucurbitacin-I (Elatericin B, JSI 124, NSC 521777) can efficiently inhibit the proliferation of GBM cells by blocking phosphorylation of STAT3, which in turn lead to the downregulation of antiapoptotic gene products and promote apoptosis. In addition, JSI-124 induced G2/M phase arrest in GBM via downregulation of cyclin B1 and cdc2 expression.<sup><a class="sref" href="#s_ref">[1]</a></sup> <sup><a class="sref" href="#s_ref">[2]</a></sup><sup><a class="sref" href="#s_ref">[3]</a></sup>

Protocol (from reference)

Cell Research:

[3]

  • Cell lines: U251 and A172 cells
  • Concentrations: 12.5, 25, 50, 100, 200, 400 and 1000 nM
  • Incubation Time: 24, 48 and 72 h
  • Method:

    Cucurbitacin I (JSI-124) induces proliferation inhibition and morphological transformation of GBM Cells. U251 cells (5 x 103/100 μl) and A127 cells(5 x 103/100 μl) were seeded in 96-well plates in triplicate, incubated overnight, treated with different concentrations of JSI-124 for 24, 48 and 72 h, then subjected to CCK-8 assay at various times to analyze proliferation of cells. Cucurbitacin I (JSI-124) dose-and time-dependently inhibited the proliferation of U251 and A172 cells.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 514.65
Formula

C30H42O7

CAS No. 2222-07-3
Storage 3 years -20°C powder
2 years -80°C in solvent

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