FLLL32

Name: FLLL32
Brand: Selleck Chemicals
SKU: S7259
Rating: 5 (5 reviews)

For research use only.

Catalog No.S7259

5 publications

Molecular Weight(MW): 464.55

FLLL32 is a potent JAK2/STAT3 inhibitor with IC50 of <5 μM.

Selleck's FLLL32 has been cited by 5 publications

Onco Targets Ther, 2020, 12;13:2215-2224

PubMed: 32210587 ( click the link to review the publication )

Biochem Biophys Res Commun, 2019, 514(3):639-644

PubMed: 31076105 ( click the link to review the publication )

Exp Ther Med, 2017, 14(4):3926-3934

PubMed: 29043002 ( click the link to review the publication )

Eur Rev Med Pharmacol Sci, 2017, 21(13):3005-3011

PubMed: 28742207 ( click the link to review the publication )

Cancer Sci, 2016, 107(7):944-54

PubMed: 27088889 ( click the link to review the publication )

2 Customer Reviews

Interleukin-6 (IL-6) secretion was inhibited by B7‐H4 silence through JAK2/signal transducer and activator of transcription 3 (STAT3) inactivation. (a) ELISA results showed that, compared with cells pretreated with control shRNA, B7‐H4 silence led to reduction of IL‐6 secretion. However, compared with cells pretreated with control shRNA and FLLL32, IL‐6 secretion in Eca109, TE1, and TE13 esophageal squamous cell carcinoma cells pretreated with B7‐H4 shRNA and FLLL32 was not significantly affected.

Cancer Sci, 2016, 107(7):944-54. . FLLL32 purchased from Selleck.

Flow cytometry detection of cell proliferation.

Eur Rev Med Pharmacol Sci, 2017, 21(13):3005-3011. FLLL32 purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description

FLLL32 is a potent JAK2/STAT3 inhibitor with IC50 of <5 μM.

Targets

JAK2 ^[1]

<5 μM

In vitro

In MDA-MB-231 breast and PANC-1 pancreatic cancer cells, FLLL32 downregulates STAT3 phosphorylation and DNA-binding activity. In MDA-MB-231 cells, FLLL32 inhibit the formation of colonies and cell invasion. ^[1] In human multiple myeloma, glioblastoma, liver cancer, and colorectal cancer cell lines, FLLL32 also leads to the inhibition of cell proliferation and the induction of caspase-3 and PARP cleavages. ^[2]

In vivo

In MDA-MB-231 xenografted mice, FLLL32 (50 mg/kg, i.p.) significantly reduces tumor burdens. ^[1] In mouse xenografts with OS-33 osteosarcoma cells, FLLL32 (50 mg/kg, i.p.) also inhibits tumor growth by targeting STAT3. ^[3]

Protocol

Kinase Assay:^[1]	- Collapse JAK2 and other human kinase activity assays: JAK2 kinase activity was assessed with the HTScan JAK2 Kinase Assay Kit per manufacturer's protocol. The possible effects of FLLL32 on the other 10 purified human protein kinases were determined by using a Kinase Profiler Assay.
Cell Research:^[1]	- Collapse Cell lines: MDA-MB-231 cell line Concentrations: ~5 μM Incubation Time: 72 hours Method: Cells are seeded in 96-well plates (3,000 per well) in triplicate and then treated with 0.5 to 5 μM of FLLL31 or FLLL32 or with 0.5 to 30 μM of curcumin for 72 h. MTT (25 μL) is added to each sample and incubated for 3.5 h. Then, 100 μL of N,N-dimethylformamide solubilization solution are added to each well. The absorbance at 450 nm is read the following day. IC50 are determined using Sigma Plot 9.0 software. (Only for Reference)
Animal Research:^[1]	- Collapse Animal Models: MDA-MB-231 xenografted mice Dosages: 50 mg/kg, daily Administration: i.p. (Only for Reference)

References

Solubility (25°C)

In vitro	DMSO	92 mg/mL (198.04 mM)
	Ethanol	25 mg/mL warmed (53.81 mM)
	Water	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download FLLL32 SDF

Molecular Weight	464.55
Formula	C₂₈H₃₂O₆
CAS No.	1226895-15-3
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	COC1=CC=C(/C=C/C(=O)C2(CCCCC2)C(=O)\C=C\C3=CC(=C(OC)C=C3)OC)C=C1OC

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

% DMSO+ % + % Tween 80+ % ddH₂O

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Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
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Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
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C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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JAK Signaling Pathway Map

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FLLL32