Puromycin 2HCl

For research use only.

Catalog No.S7417

57 publications

CAS No. 58-58-2

Puromycin 2HCl is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.

Selleck's Puromycin 2HCl has been cited by 57 publications

Cell, 2018, 174(6):1477-1491

PubMed: 30146158 ( click the link to review the publication )

Nucleic Acids Res, 2021, gkab034

PubMed: 33503260 ( click the link to review the publication )

Theranostics, 2021, 11(6):2966-2986

PubMed: 33456583 ( click the link to review the publication )

Oncogenesis, 2021, 10(1):6

PubMed: 33423038 ( click the link to review the publication )

Genome Biol, 2020, 21(1):269

PubMed: 33143730 ( click the link to review the publication )

Autophagy, 2020, 15:1-13

PubMed: 32264736 ( click the link to review the publication )

Cell Rep, 2020, 30(2):497-509

PubMed: 31940492 ( click the link to review the publication )

Oncogene, 2020, 39(21):4212-4226

PubMed: 32251364 ( click the link to review the publication )

Stem Cell Res, 2020, 49:102052

PubMed: 33130469 ( click the link to review the publication )

Cancer Lett, 2020, 469:380-389

PubMed: 31730901 ( click the link to review the publication )

Cancers (Basel), 2020, 20;12(2) pii: E493

PubMed: 32093281 ( click the link to review the publication )

Cancers (Basel), 2020, 23;12(3):758

PubMed: 32210086 ( click the link to review the publication )

Oncogenesis, 2020, 29;9(4):42

PubMed: 32350243 ( click the link to review the publication )

J Cell Mol Med, 2020, 24(7):4223-4232

PubMed: 32105413 ( click the link to review the publication )

J Cell Mol Med, 2020, 24(5):2847-2856

PubMed: 31778016 ( click the link to review the publication )

Biomed Res Int, 2020, 2020:9372961

PubMed: 32908925 ( click the link to review the publication )

Int J Genomics, 2020, 2020:3750673

PubMed: 32509843 ( click the link to review the publication )

Cell Biol Int, 2020, 10.1002/cbin.11507

PubMed: 33245175 ( click the link to review the publication )

Oncol Lett, 2020, 20(6):365

PubMed: 33133265 ( click the link to review the publication )

Pathogens, 2020, 9(6):443

PubMed: 32512928 ( click the link to review the publication )

Theranostics, 2020, 10(17):7906-7920

PubMed: 32685028 ( click the link to review the publication )

Exp Cell Res, 2020, 389(1):111894

PubMed: 32035950 ( click the link to review the publication )

J Craniomaxillofac Surg, 2020, 48(1):105-110

PubMed: 31884029 ( click the link to review the publication )

Onco Targets Ther, 2020, 13:1413-1419

PubMed: 32110040 ( click the link to review the publication )
J Med Genet, 2020, jmedgenet-2019-106706

PubMed: 32737136 ( click the link to review the publication )

Onco Targets Ther, 2020, 13:6539-6551

PubMed: 32753892 ( click the link to review the publication )

Am J Transl Res, 2020, 12(7):3461-3475

PubMed: 32774712 ( click the link to review the publication )

J Clin Invest, 2019, 129(6):2333-2350

PubMed: 31063987 ( click the link to review the publication )

Nat Commun, 2019, 10(1):5792

PubMed: 31857589 ( click the link to review the publication )

Cell Rep, 2019, 29(5):1311-1322.e5

PubMed: 31665642 ( click the link to review the publication )

Cell Death Differ, 2019, 10.1038/s41418-019-0402-x

PubMed: 31406304 ( click the link to review the publication )

Mol Cancer, 2019, 18(1):151

PubMed: 31672157 ( click the link to review the publication )

Oncogene, 2019, 38(2):180-193

PubMed: 30093630 ( click the link to review the publication )

PLoS Pathog, 2019, 15(6):e1007795

PubMed: 31170267 ( click the link to review the publication )

Cells, 2019, 8(11)E1335

PubMed: 31671755 ( click the link to review the publication )

Mol Ther Methods Clin Dev, 2019, 14:47-55

PubMed: 31309127 ( click the link to review the publication )

J Cell Mol Med, 2019, 23(7):4545-4558

PubMed: 31062473 ( click the link to review the publication )

Int J Mol Sci, 2019, 20(19)E4852

PubMed: 31574902 ( click the link to review the publication )

J Biol Chem, 2019, 294(3):772-782

PubMed: 30429217 ( click the link to review the publication )

Exp Cell Res, 2019, 376(2):105-113

PubMed: 30772381 ( click the link to review the publication )

Exp Cell Res, 2019, 10.1016/j.yexcr.2019.06.006

PubMed: 31194977 ( click the link to review the publication )

Oncol Lett, 2019, 17(3):2969-2975

PubMed: 30867731 ( click the link to review the publication )

Nucleic Acids Res, 2019, 47(8):4124-4135

PubMed: 30783677 ( click the link to review the publication )

Front Oncol, 2019, 9:80

PubMed: 30873379 ( click the link to review the publication )

Cell Commun Signal, 2018, 16(1):65

PubMed: 30286765 ( click the link to review the publication )

Mol Med Rep, 2018, 17(4):6116-6121

PubMed: 29436634 ( click the link to review the publication )

Theranostics, 2018, 8(15):4050-4061

PubMed: 30128035 ( click the link to review the publication )

FASEB J, 2018, 10.1096/fj.201701351

PubMed: 29906248 ( click the link to review the publication )
Biochim Biophys Acta Mol Basis Dis, 2018, 1864(5 Pt A):1754-1769

PubMed: 29510196 ( click the link to review the publication )

Gynecol Oncol, 2018, 149(2):341-349

PubMed: 29572031 ( click the link to review the publication )

Lab Invest, 2018, 98(12):1627-1641

PubMed: 30089852 ( click the link to review the publication )

Int J Mol Sci, 2018, 19(5)

PubMed: 29786666 ( click the link to review the publication )

Nat Commun, 2017, 8(1):244

PubMed: 28811474 ( click the link to review the publication )

Cell Death Dis, 2017, 8(9):e3048

PubMed: 28906492 ( click the link to review the publication )

Oncotarget, 2017, 8(14):23401-23413

PubMed: 28423580 ( click the link to review the publication )

Oncotarget, 2017, 8(17):28865-28879

PubMed: 28427200 ( click the link to review the publication )

Am J Transl Res, 2016, 8(9):3986-3994

PubMed: 27725879 ( click the link to review the publication )

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description

Puromycin 2HCl is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.

In vitro

The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. ^[1] Puromycin can be used for the selection of recombinant cells from noncultured cells. ^[2]

Cell Data

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
K562	M3zYWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	MoHaO\|IhcA>?	NGrxcnFIem:5dHigbY5pcWKrdHnvckBw\iCmb4jvdpVjcWOrbj3y[ZNqe3SjboSgbJVu[W5iS{W2NkBk\WyuczDh[pRmeiB5MjDodpMh[nliU2LCJIF{e2G7LDDJR\|UxRTJ4LkizJO69VS5?	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDNzM{OwO{c,OTh\|MUOzNFc9N2F-
HepG2	NYO0fI5pT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	M1PwbFczKGh?	NELJTGxIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvOjNizszNMi=>	NUHSWHljRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizNVM{ODdpPkG4N\|E{OzB5PD;hQi=>
K562	NGTXbHZIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	NXPL[ld1PzJiaB?=	M172[2dzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGs2PjJiY3XscJMh[W[2ZYKgO\|IhcHK\|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlIzKM7:TT6=	MoDoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|MUOzNFcoRjF6M{GzN\|A4RC:jPh?=
HepG2	MYLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	NUL4SnpCPzJiaB?=	NES2[3pIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVAvOjFizszNMi=>	M4rKclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUGyPVg1Lz5zOEWxNlk5PDxxYU6=
Huh7	Mn7BR5l1d3SxeHnjbZR6KGG\|c3H5		M{DtcG5QXkGUVFnTPkBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBp\XCjdH;j[YxtfWyjcjDjZZJkcW6xbXGgZ4VtdCCuaX7lJEhJfWh5KTygR2M2OD14Lk[zJO69VS5?	NWPEbndCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1O\|k4QDNpPkG4OVc6Pzh\|PD;hQi=>
HEK239	M2XPbGN6fG:2b4jpZ4l1gSCjc4PhfS=>	NGfOVHQ4OiCq	MYPDfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{M{mgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KEGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwQVAvPDJizszNMi=>	MnXyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3NUiwOlAoRjJyNUW4NFYxRC:jPh?=
J774A1	MkG4R5l1d3SxeHnjbZR6KGG\|c3H5		MVnDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W\|ZTDKO\|c1STFiY3XscJMh[nlidIL5dIFvKGKudXWg[ZhkdHW\|aX;uJIF{e2G7LDDJR\|UxRTFyIN88UU4>	NHT4O2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmzOFY{Pid-MkK5N\|Q3OzZ:L3G+
KB3-1	NH7rbFBIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	Mm[zO\|IhcHK\|	MXHHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDLRlMuOSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNE4zOSEQvF2u	NYi5ZYR[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizNVM{ODdpPkG4N\|E{OzB5PD;hQi=>
HepG2/Dox	MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	NWLjNXB3PzJiaILz	NXXBe4ozT3Kxd4ToJIlvcGmkaYTpc44hd2ZibYXseIllenWpIILld4l{fGGwdDDoeY1idiCKZYDHNk9Fd3hiY3XscJMh[W[2ZYKgO\|IhcHK\|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFIxNjJizszNMi=>	M1XJflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUGyPVg1Lz5zOEWxNlk5PDxxYU6=
HEK293	M3HQdWN6fG:2b4jpZ4l1gSCjc4PhfS=>	NIPrfJA4OiCqcoO=	NIrKclhEgXSxdH;4bYNqfHliYXfhbY5{fCCKRVuyPVMh[2WubIOgZZN{\XO\|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiN{KgbJJ{KGK7IILld4F7fXKrbj3iZZNm\CCybHH0[UBz\WGmZYKgZY5idHm\|aYOsJGlEPTBiPTCwMlM3OSEQvF2u	NGnP[Wc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[1NVU{Pyd-Mk[2OVE2Ozd:L3G+
HEK293	NUjS[m9CS3m2b4TvfIlkcXS7IHHzd4F6	NU\id5FEPzJiaILz	MYXDfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IHHsZY1ieiCkbIXlJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IECuN\|UxPSEQvF2u	M37nNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5ME[wO\|Y{Lz5{N{C2NFc3OzxxYU6=
A549	NHzvXFlEgXSxdH;4bYNqfHliYYPzZZk>		NWDa[o55S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyxiSVO1NEA:KDBwNjFOwG0v	NF2yU\|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C4PVIyPCd-MkewPFkzOTR:L3G+
PC3	MYfDfZRwfG:6aXPpeJkh[XO\|YYm=		M1LEZ2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBEOyClZXzsd{whUUN3MDC9JFAvPyEQvF2u	NIjj[Xk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C4PVIyPCd-MkewPFkzOTR:L3G+
HEK293	MkLDR5l1d3SxeHnjbZR6KGG\|c3H5		M{fFPGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEiHS{K5N{Bk\WyuczDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3MDC9JFAvODByM{Wg{txONg>?	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzJzMkC3NEc,Ojd{MUKwO\|A9N2F-
HEK293	M1rVV2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7	M2rMU\|czKGi{cx?=	NF\iNppIem:5dHigbY5pcWKrdHnvckBw\iCKRVuyPVMh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IGDy[ZN1d0KudXWgd5RicW6rbnegZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7LDDJR\|UxKD1iMD6zOkDPxE1w	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDByMUC2O{c,OjhyMEGwOlc9N2F-
HEK293	Mlm0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		NGXMblBIem:5dHigbY5pcWKrdHnvckBw\iCKRVuyPVMh[2WubIOsJGlEPTBiPTCwMlM6QSEQvF2u	NV;tVnhrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0NFAzOzFpPkK4OFAxOjNzPD;hQi=>
SU-DHL10	NIf2WXREgXSxdH;4bYNqfHliYYPzZZk>	MUO3NkBpenN?	MUDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTWU1FUExzMDDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB5MjDodpMh[nliQ3XscHRqfHKnLVfsc{BtfW2rbnXzZ4VvfCCjc4PhfUwhS0N3MDC9JFAvOTFizszNMi=>	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV{M{GwN{c,Ojh3MkOxNFM9N2F-
MIAPaCa2	MVHDfZRwfG:6aXPpeJkh[XO\|YYm=	MoHwO\|IhcHK\|	Ml;oR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWlCWGGFYUKgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IFPlcIxVcXS{ZT3HcI8hdHWvaX7ld4NmdnRiYYPzZZktKEOFNUCgQUAxNjJizszNMi=>	NIPYPZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEWyN\|ExOyd-Mki1NlMyODN:L3G+
MDA-MB-231	NF3PUGpEgXSxdH;4bYNqfHliYYPzZZk>	Mny5O\|IhcHK\|	NEjMe3lEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIF{e2W\|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFczKGi{czDifUBE\WyuVHn0doUuT2yxIHz1cYlv\XOlZX70JIF{e2G7LDDDR\|UxKD1iMD60PEDPxE1w	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV{M{GwN{c,Ojh3MkOxNFM9N2F-
HEK293	MlntR5l1d3SxeHnjbZR6KGG\|c3H5	NG[3fG04OiCqcoO=	MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA4OiCqcoOgZpkhSWyjbXHyJIJtfWViYYPzZZktKEmFNUCgQUAxNjR4IN88UU4>	MnvPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5N{S0NlcoRjJ6N{e0OFI4RC:jPh?=
HL60	MV7BcpRqeGG{YYPpeIlkKGG\|c3H5	NVzBUo9HPzJiaILz	Mn6wRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCKTE[wJINmdGy\|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFXDOVAhRSByLkC1OUDPxE1w	M2Hj[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OECzNFQ4Lz5{OEiwN\|A1PzxxYU6=
MOLT4	MmDjRY51cXCjcnHzbZRq[yCjc4PhfS=>	NIPRUWk4OiCqcoO=	MWfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2RTGS0JINmdGy\|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFXDOVAhRSByLkG3JO69VS5?	Mo\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6MEOwOFcoRjJ6OECzNFQ4RC:jPh?=
Vero	M1nadmN6fG:2b4jpZ4l1gSCjc4PhfS=>	NIjSV3M4OiCqcoO=	MXXDfZRwfG:6aXPpeJkh[WejaX7zeEBC\nKrY3HuJIdz\WWwIH3vcotmgSCYZYLvJINmdGy\|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJBzd2yrZnXyZZRqd25iYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiQ1O1NEA:KDJwODFOwG0v	NWXwepBVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki4NFMxPDdpPkK4PFA{ODR5PD;hQi=>
HEK293	NVnQOm1MS3m2b4TvfIlkcXS7IHHzd4F6	NEHXVWs4OiCqcoO=	Ml3yR5l1d3SxeHnjbZR6KGGpYXnud5QhUEWNMkmzJINmdGy\|IHHmeIVzKDd{IHjyd{BjgSC{ZYPhfpVzcW5iZInlJIJie2WmIHHzd4F6NCCLQ{WwJF0hOC52NTFOwG0v	NYnSTYFNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyN\|Y1QTJpPkK5NlM3PDl{PD;hQi=>
HEK293T	MXPDfZRwfG:6aXPpeJkh[XO\|YYm=	NYjuNGtDPzJiaILz	MWnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDISWszQTOWIHPlcIx{KGG\|c3Xzd4VlKGG\|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvR3zvJJJm[WenboSgZoF{\WRibIXtbY5me2OnbnPlJIF{e2G7LDDDR\|UxKD1iMD60NkDPxE1w	MlvrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2Nkm1O\|UoRjJ7NE[5OVc2RC:jPh?=
HCT8	NEPZO4dCdnSrcHHyZZNqfGmlIHHzd4F6	NFS2dHQ1QCCqcoO=	MmPORY51cXCjcnHzbZRq[yCjY4Tpeol1gSCjZ3HpcpN1KEO{eYD0c5Nxd3KrZHn1cUBx[XK4dX2gRmdHKGmwZnXjeIVlKGmwIHj1cYFvKEiFVEigZ4VtdHNiaX7jeYJifGWmIH\vdkA1QCCqcoOgZpkhTkmWQz;ERXBKKHO2YXnubY5oKGKjc3XkJIZtfW:{ZYPj[Y5k\SCjc4PhfUwhTUN3MDC9JFAvPTVizszNMi=>	NYPFWpNDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0Olk2PzVpPkK5OFY6PTd3PD;hQi=>
HCT8	NUXZc5FESW62aYDhdoF{cXSrYzDhd5NigQ>?	NYDpUmZYPDhiaILz	Ml:xRY51cXCjcnHzbZRq[yCjY4Tpeol1gSCjZ3HpcpN1KEO{eYD0c5Nxd3KrZHn1cUBx[XK4dX2gV3BNKGmwZnXjeIVlKGmwIHj1cYFvKEiFVEigZ4VtdHNiaX7jeYJifGWmIH\vdkA1QCCqcoOgZpkhTkmWQz;ERXBKKHO2YXnubY5oKGKjc3XkJIZtfW:{ZYPj[Y5k\SCjc4PhfUwhTUN3MDC9JFAvPiEQvF2u	MofWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2Nkm1O\|UoRjJ7NE[5OVc2RC:jPh?=
HepG2	MULDfZRwfG:6aXPpeJkh[XO\|YYm=	M17ueFczKGi{cx?=	M2\6Z2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGG\|c3Xzd4VlKGG\|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvR3zvJJJm[WenboSgZoF{\WRibIXtbY5me2OnbnPlJIF{e2G7LDDDR\|UxKD1iMD64N{DPxE1w	Mn3lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2Nkm1O\|UoRjJ7NE[5OVc2RC:jPh?=
HCT8	NIPLeW1EgXSxdH;4bYNqfHliYYPzZZk>		NVGzN4tuS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWODDj[YxteyxiRVO1NEA:KDZwMTFOwG0v	M2OxdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NE[5OVc2Lz5{OUS2PVU4PTxxYU6=
HEK293	M2PPfGN6fG:2b4jpZ4l1gSCjc4PhfS=>	MkT5O\|IhcHK\|	NFe1cZREgXSxdH;4bYNqfHliYXfhbY5{fCCKRVuyPVMh[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKGe{b4f0bEBi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO\|YYmsJGlEPTBiPTCwMlQ3KM7:TT6=	NXfzSYliRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1N\|M3OTFpPkK5OVM{PjFzPD;hQi=>
HEK293	MoS1R5l1d3SxeHnjbZR6KGG\|c3H5	NWTvUWZoPzJiaILz	NFHKWodEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJTUt{OUOgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KHKnc3H6eZJqdiCmeXWgZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7LDDJR\|UxKD1iMD60JO69VS5?	NYHteItiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm5OlkzPjJpPkK5PVY6OjZ{PD;hQi=>
A549	M{S5VmN6fG:2b4jpZ4l1gSCjc4PhfS=>	MWe0PEBpenN?	Mn3IR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOsJGlEPTBiPTCwMlMh\|ryPLh?=	NWPabmhlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C0NVg4PjNpPkOwOFE5PzZ\|PD;hQi=>
PC3	MV7DfZRwfG:6aXPpeJkh[XO\|YYm=	MYO0PEBpenN?	MWjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDQR\|Mh[2WubIOgZZN{\XO\|ZXSgZZMh\GWlcnXhd4UhcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK\|LDDJR\|UxKD1iMD6zJO69VS5?	NGLFcG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MESxPFc3Oyd-M{C0NVg4PjN:L3G+

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Cyclin D1 / CDK4; PubMed: 31022952 Cancers (Basel) (C) Effect of puromycin on cyclin D1 and CDK4 in a concentration-dependent manner in HCT116 and H1299 cells. Cells were treated with puromycin (0.25 or 0.5 μg/mL) for 24 h and subjected to Western blotting with antibodies of cyclin D1, CDK4 and β actin. (D) Effect of puromycin on cyclin D1 and CDK4 in a time-dependent manner in HCT116 and H1299 cells. Cells were treated with 0.5 μg/mL puromycin for 3, 6, 12 or 24 h and subjected to Western blotting with antibodies of cyclin D1, CDK4, and β-actin. PARP / Bcl-xL / Bcl-2 / p-Akt / Akt; PubMed: 31022952 Cancers (Basel) (E) Effect of puromycin on Poly (ADP-ribose) polymerase (PARP), Bcl-xL, Bcl-2, AKT, and p-AKT (v-akt murine thymoma viral oncogene homolog) in a concentration-dependent manner in HCT116 and H1299 cells. Cells were treated with puromycin (0.25 or 0.5 μg/mL) for 24 h and subjected to Western blotting with antibodies of PARP, Bcl-xL, Bcl-2, p-AKT, AKT, and β-actin. (F) Effect of puromycin on PARP, Bcl-xL, Bcl-2, AKT, and p-AKT in a time-dependent manner in HCT116 and H1299 cells. Cells were treated with 0.5 μg/mL of puromycin for 3, 6, 12 or 24 h and subjected to Western blotting with antibodies of PARP, Bcl-xL, Bcl-2, p-AKT, AKT, and β-actin. p53 / p21 / c-Myc; PubMed: 31022952 Cancers (Basel) Effect of puromycin on p53, p21, and c-Myc in a concentration and time-dependent manner in HCT116 cells. HCT116 cells were exposed to various concentrations of puromycin (0.25 or 0.5 μg/mL) for 24 h and then subjected to Western blotting with antibodies of p53, p21, and c-Myc. Also, HCT116 cells were treated with 0.5 μg/mL puromycin for 6, 12, or 24 h and subjected to Western blotting.	31022952
Growth inhibition assay	Cell viability; PubMed: 31022952 Cancers (Basel) Cytotoxicity of puromycin in HCT116, SW620, HCT15, and H1299 cells in a concentration-dependent manner by 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Data represent means ± SD. , p < 0.05, , p < 0.01, **, p< 0.001 vs. untreated control.	31022952

Protocol

References

Solubility (25°C)

In vitro	DMSO	100 mg/mL warmed (183.67 mM)
	Water	100 mg/mL warmed (183.67 mM)
	Ethanol	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Puromycin 2HCl SDF

Molecular Weight	544.43
Formula	C₂₂H₂₉N₇O₅.2HCl
CAS No.	58-58-2
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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mg/kg

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Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

% DMSO+ % + % Tween 80+ % ddH₂O

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Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Puromycin 2HCl