Puromycin 2HCl

Catalog No.S7417

Puromycin 2HCl Chemical Structure

Molecular Weight(MW): 544.43

Puromycin 2HCl is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.

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Cited by 16 publications

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Biological Activity

Description Puromycin 2HCl is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.
In vitro

The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. [1] Puromycin can be used for the selection of recombinant cells from noncultured cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HepG2 cells NF6xOoVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVf1U5dMPzJiaB?= MXjHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC3NkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUCuNlEh|ryPLh?= NWDZR4ZmOTh3MUK5PFQ>
human K562 cells MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkjNO|IhcA>? NYn2d3hUT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUzV4MjDj[YxteyCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVAvOjJizszNMi=> MVKxPFMyOzNyNx?=
human HepG2 cells MoTWS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnzsO|IhcA>? MUPHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUCuNlMh|ryPLh?= NFHCcXAyQDNzM{OwOy=>
HEK239 cells M4TKbWN6fG:2b4jpZ:Kh[XO|YYm= MUe3NkBp MUPDfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{M{mgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KEGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwQVAvPDJizszNMi=> M3HTOVIxPTV6ME[w
human hepatocellular carcinoma cell line (Huh7) MkXIR5l1d3SxeHnjxsBie3OjeR?= MmHOUm9XSVKWSWO6JGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIhmeGG2b3PlcIx2dGG{IHPhdoNqdm:vYTDj[YxtKGyrbnWgLGh2cDdrLDDDR|UxRTZwNkOg{txONg>? NXmxc4ZzOTh3N{m3PFM>
mouse J774A1 cells NIPRd3NEgXSxdH;4bYPDqGG|c3H5 MmntR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgTlc4PEFzIHPlcIx{KGK7IITyfZBidiCkbIXlJIV5[2y3c3nvckBie3OjeTygTWM2OD1zMDFOwG0v NGq3R2QzOjl|NE[zOi=>
human K562 cells MnzTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlztO|IhcA>? M1nK[Gdzd3e2aDDpcohq[mm2aX;uJI9nKGSxeH;yeYJq[2mwLYLld4l{fGGwdDDoeY1idiCNNU[yJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCVUlKgZZN{[XluIFnDOVA:OjZwOEOg{txONg>? NHzQRmYyQDNzM{OwOy=>

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot
Cyclin D1 / CDK4; 

PubMed: 31022952     


(C) Effect of puromycin on cyclin D1 and CDK4 in a concentration-dependent manner in HCT116 and H1299 cells. Cells were treated with puromycin (0.25 or 0.5 μg/mL) for 24 h and subjected to Western blotting with antibodies of cyclin D1, CDK4 and β actin. (D) Effect of puromycin on cyclin D1 and CDK4 in a time-dependent manner in HCT116 and H1299 cells. Cells were treated with 0.5 μg/mL puromycin for 3, 6, 12 or 24 h and subjected to Western blotting with antibodies of cyclin D1, CDK4, and β-actin.

PARP / Bcl-xL / Bcl-2 / p-Akt / Akt; 

PubMed: 31022952     


(E) Effect of puromycin on Poly (ADP-ribose) polymerase (PARP), Bcl-xL, Bcl-2, AKT, and p-AKT (v-akt murine thymoma viral oncogene homolog) in a concentration-dependent manner in HCT116 and H1299 cells. Cells were treated with puromycin (0.25 or 0.5 μg/mL) for 24 h and subjected to Western blotting with antibodies of PARP, Bcl-xL, Bcl-2, p-AKT, AKT, and β-actin. (F) Effect of puromycin on PARP, Bcl-xL, Bcl-2, AKT, and p-AKT in a time-dependent manner in HCT116 and H1299 cells. Cells were treated with 0.5 μg/mL of puromycin for 3, 6, 12 or 24 h and subjected to Western blotting with antibodies of PARP, Bcl-xL, Bcl-2, p-AKT, AKT, and β-actin.

p53 / p21 / c-Myc; 

PubMed: 31022952     


Effect of puromycin on p53, p21, and c-Myc in a concentration and time-dependent manner in HCT116 cells. HCT116 cells were exposed to various concentrations of puromycin (0.25 or 0.5 μg/mL) for 24 h and then subjected to Western blotting with antibodies of p53, p21, and c-Myc. Also, HCT116 cells were treated with 0.5 μg/mL puromycin for 6, 12, or 24 h and subjected to Western blotting.

31022952
Growth inhibition assay
Cell viability; 

PubMed: 31022952     


Cytotoxicity of puromycin in HCT116, SW620, HCT15, and H1299 cells in a concentration-dependent manner by 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Data represent means ± SD. *, p < 0.05, **, p < 0.01, ***, p< 0.001 vs. untreated control.

31022952

Protocol

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (183.67 mM)
Water 100 mg/mL warmed (183.67 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 544.43
Formula

C22H29N7O5.2HCl
CAS No. 58-58-2
Storage powder
in solvent
Synonyms N/A

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID