Puromycin 2HCl

Catalog No.S7417

Puromycin 2HCl Chemical Structure

Molecular Weight(MW): 544.43

Puromycin 2HCl is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.

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Cited by 10 publications

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Biological Activity

Description Puromycin 2HCl is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.
In vitro

The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. [1] Puromycin can be used for the selection of recombinant cells from noncultured cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HepG2 cells Mn;uS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NH;1XJg4OiCq NVvzNmxoT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF0xNjJzIN88UU4> MkL6NVg2OTJ7OES=
human K562 cells NH;v[nlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1rGVVczKGh? M1PUXWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGs2PjJiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlIzKM7:TT6= MmTsNVg{OTN|MEe=
human HepG2 cells NX3aN5FOT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWW3NkBp M1qySWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NE4zOyEQvF2u M4q1flE5OzF|M{C3
HEK239 cells M1jrRmN6fG:2b4jpZ:Kh[XO|YYm= MXu3NkBp M320RmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEiHS{KzPUBk\WyuczDh[pRmeiB5MjDodpMh[nliQXzhcYFzKGKudXWgZZN{[XluIFnDOVA:OC52MjFOwG0v MWSyNFU2QDB4MB?=
human hepatocellular carcinoma cell line (Huh7) NW\1OIoxS3m2b4TvfIlkyqCjc4PhfS=> NGLtfItPV1[DUmTJV|ohS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hcGWyYYTvZ4VtdHWuYYKgZ4Fz[2mwb33hJINmdGxibHnu[UApUHWqNzmsJGNEPTB;Nj62N{DPxE1w M135cVE5PTd7N{iz
mouse J774A1 cells Mli4R5l1d3SxeHnjxsBie3OjeR?= NHG4cpREgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBLPzd2QUGgZ4VtdHNiYomgeJJ6eGGwIHLseYUh\XilbIXzbY9vKGG|c3H5MEBKSzVyPUGwJO69VS5? NGnnPIIzOjl|NE[zOi=>
human K562 cells M3fBfGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1m1eFczKGh? NXK2R3B7T3Kxd4ToJIlvcGmkaYTpc44hd2ZiZH;4c5J2[mmlaX6tdoV{cXO2YX70JIh2dWGwIFu1OlIh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IGPSRkBie3OjeTygTWM2OD1{Nj64N{DPxE1w NFjZZ5cyQDNzM{OwOy=>

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot
Cyclin D1 / CDK4; 

PubMed: 31022952     


(C) Effect of puromycin on cyclin D1 and CDK4 in a concentration-dependent manner in HCT116 and H1299 cells. Cells were treated with puromycin (0.25 or 0.5 μg/mL) for 24 h and subjected to Western blotting with antibodies of cyclin D1, CDK4 and β actin. (D) Effect of puromycin on cyclin D1 and CDK4 in a time-dependent manner in HCT116 and H1299 cells. Cells were treated with 0.5 μg/mL puromycin for 3, 6, 12 or 24 h and subjected to Western blotting with antibodies of cyclin D1, CDK4, and β-actin.

PARP / Bcl-xL / Bcl-2 / p-Akt / Akt; 

PubMed: 31022952     


(E) Effect of puromycin on Poly (ADP-ribose) polymerase (PARP), Bcl-xL, Bcl-2, AKT, and p-AKT (v-akt murine thymoma viral oncogene homolog) in a concentration-dependent manner in HCT116 and H1299 cells. Cells were treated with puromycin (0.25 or 0.5 μg/mL) for 24 h and subjected to Western blotting with antibodies of PARP, Bcl-xL, Bcl-2, p-AKT, AKT, and β-actin. (F) Effect of puromycin on PARP, Bcl-xL, Bcl-2, AKT, and p-AKT in a time-dependent manner in HCT116 and H1299 cells. Cells were treated with 0.5 μg/mL of puromycin for 3, 6, 12 or 24 h and subjected to Western blotting with antibodies of PARP, Bcl-xL, Bcl-2, p-AKT, AKT, and β-actin.

p53 / p21 / c-Myc; 

PubMed: 31022952     


Effect of puromycin on p53, p21, and c-Myc in a concentration and time-dependent manner in HCT116 cells. HCT116 cells were exposed to various concentrations of puromycin (0.25 or 0.5 μg/mL) for 24 h and then subjected to Western blotting with antibodies of p53, p21, and c-Myc. Also, HCT116 cells were treated with 0.5 μg/mL puromycin for 6, 12, or 24 h and subjected to Western blotting.

31022952
Growth inhibition assay
Cell viability; 

PubMed: 31022952     


Cytotoxicity of puromycin in HCT116, SW620, HCT15, and H1299 cells in a concentration-dependent manner by 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Data represent means ± SD. *, p < 0.05, **, p < 0.01, ***, p< 0.001 vs. untreated control.

31022952

Protocol

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (183.67 mM)
Water 100 mg/mL warmed (183.67 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 544.43
Formula

C22H29N7O5.2HCl
CAS No. 58-58-2
Storage powder
in solvent
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID