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Puromycin 2HCl

Name: Puromycin 2HCl
Brand: Selleck Chemicals
SKU: S7417
Rating: 5 (203 reviews)

Synonyms: CL13900 2HCl

Catalog No.S7417 Purity:99.89%

Puromycin 2HCl is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinated expression of genes during cell differentiation.

Puromycin 2HCl Chemical Structure

CAS No. 58-58-2

Purity & Quality Control

Batch: Purity: 99.89%

99.89

Products often used together with Puromycin 2HCl

Blasticidin S HCl

Puromycin and Blasticidin S inhibits the proliferation and cell viability of metastatic castration-resistant prostate cancer (mCRPC) cells PC3/DU145.

Oseni SO, et al. bioRxiv (2021): 2021-08.

Melatonin

Melatonin potentiates puromycin-induced apoptosis with caspase-3 and AMPK activation in HL-60 cells.

Koh W, et al. J Pineal Res. 2011 May;50(4):367-73.

Puromycin 2HCl Related Products

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Related Compound Libraries	Anti-infection Compound Library Antibiotics compound Library Antiviral Compound Library Anti-parasitic Compound Library Gut Microbial Metabolite Library	Click to Expand

Cell Data

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
K562	Growth inhibition assay	72 h	Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay, IC50=26.83 μM.	18313307
HepG2	Growth inhibition assay	72 h	Growth inhibition of human HepG2 cells after 72 hrs by MTT assay, IC50=0.23 μM.	18313307
K562	Growth inhibition assay	72 h	Growth inhibition of human K562 cells after 72 hrs by SRB assay, IC50=0.22 μM.	18313307
HepG2	Growth inhibition assay	72 h	Growth inhibition of human HepG2 cells after 72 hrs by SRB assay, IC50=0.21 μM.	18512984
Huh7	Cytotoxicity assay		NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7), CC50=6.63 μM.	18579783
HEK239	Cytotoxicity assay	72 h	Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay, IC50=0.42 μM.	20558060
J774A1	Cytotoxicity assay		Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay, IC50=10 μM.	22934636
KB3-1	Growth inhibition assay	72 hrs	Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay, IC50 = 0.21 μM.	18313307
HepG2/Dox	Growth inhibition assay	72 hrs	Growth inhibition of multidrug resistant human HepG2/Dox cells after 72 hrs by SRB assay, IC50 = 20.2 μM.	18512984
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis, IC50 = 0.361 μM.	26651537
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay, IC50 = 0.3505 μM.	27060763
A549	Cytotoxicity assay		Cytotoxicity against human A549 cells, IC50 = 0.6 μM.	27089214
PC3	Cytotoxicity assay		Cytotoxicity against human PC3 cells, IC50 = 0.7 μM.	27089214
HEK293	Cytotoxicity assay		Cytotoxicity against HEK293 cells by alamar blue assay, IC50 = 0.00035 μM.	27212070
HEK293	Growth inhibition assay	72 hrs	Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay, IC50 = 0.36 μM.	28001067
HEK293	Growth inhibition assay		Growth inhibition of HEK293 cells, IC50 = 0.399 μM.	28400231
SU-DHL10	Cytotoxicity assay	72 hrs	Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.11 μM.	28523103
MIAPaCa2	Cytotoxicity assay	72 hrs	Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.2 μM.	28523103
MDA-MB-231	Cytotoxicity assay	72 hrs	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.48 μM.	28523103
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay, IC50 = 0.46 μM.	28774427
HL60	Antiparasitic assay	72 hrs	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay, EC50 = 0.055 μM.	28803047
MOLT4	Antiparasitic assay	72 hrs	Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay, EC50 = 0.17 μM.	28803047
Vero	Cytotoxicity assay	72 hrs	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay, CC50 = 2.8 μM.	28803047
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay, IC50 = 0.45 μM.	29236492
HEK293T	Cytotoxicity assay	72 hrs	Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.42 μM.	29469575
HCT8	Antiparasitic assay	48 hrs	Antiparasitic activity against Cryptosporidium parvum BGF infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.55 μM.	29469575
HCT8	Antiparasitic assay	48 hrs	Antiparasitic activity against Cryptosporidium parvum SPL infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.6 μM.	29469575
HepG2	Cytotoxicity assay	72 hrs	Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.83 μM.	29469575
HCT8	Cytotoxicity assay		Cytotoxicity against human HCT8 cells, EC50 = 6.1 μM.	29469575
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay, IC50 = 0.46 μM.	29533611
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay, IC50 = 0.4 μM.	29969262
A549	Cytotoxicity assay	48 hrs	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM.	30418763
PC3	Cytotoxicity assay	48 hrs	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM.	30418763
Click to View More Cell Line Experimental Data

Biological Activity

Description	Puromycin 2HCl is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinated expression of genes during cell differentiation.

In vitro
In vitro	The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. ^[1] Puromycin can be used for the selection of recombinant cells from noncultured cells. ^[2]
Cell Research	Cell lines	HepG2 cells
	Concentrations	1.6 μM
	Incubation Time	72 h
	Method	Cells were treated with different concentrations of drug for 72 h.
Experimental Result Images	Methods	Biomarkers	Images	PMID
	Western blot	Cyclin D1 / CDK4 PARP / Bcl-xL / Bcl-2 / p-Akt / Akt p53 / p21 / c-Myc		31022952
	Growth inhibition assay	Cell viability		31022952

In Vivo
In vivo	Puromycin (aminonucleoside) inhibits protein synthesis leading to proteinuria & glomerulosclerosis/nephrosis.^[4]
Animal Research	Animal Models	Male Sprague-Dawley rats
	Dosages	5 mg/100 g
	Administration	i.v.

References

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Chemical Information & Solubility

Molecular Weight	544.43	Formula	C₂₂H₂₉N₇O₅.2HCl
CAS No.	58-58-2	SDF	Download Puromycin 2HCl SDF
Smiles	CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (183.67 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Clear solution	saline	100.000mg/ml (183.68mM)	Taking the 1 mL working solution as an example, add 100 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	saline	100.000mg/ml (183.68mM)	Taking the 1 mL working solution as an example, add 100 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	saline	100.000mg/ml (183.68mM)	Taking the 1 mL working solution as an example, add 100 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	saline	100.000mg/ml (183.68mM)	Taking the 1 mL working solution as an example, add 100 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	saline	100.000mg/ml (183.68mM)	Taking the 1 mL working solution as an example, add 100 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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