Puromycin 2HCl

For research use only.

Catalog No.S7417 Synonyms: CL13900

65 publications

Puromycin 2HCl Chemical Structure

CAS No. 58-58-2

Puromycin 2HCl (CL13900) is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.

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Selleck's Puromycin 2HCl has been cited by 65 publications

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Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Puromycin 2HCl (CL13900) is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.
In vitro

The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. [1] Puromycin can be used for the selection of recombinant cells from noncultured cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K562 MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mmm0O|IhcA>? NH7TcWtIem:5dHigbY5pcWKrdHnvckBw\iCmb4jvdpVjcWOrbj3y[ZNqe3SjboSgbJVu[W5iS{W2NkBk\WyuczDh[pRmeiB5MjDodpMh[nliU2LCJIF{e2G7LDDJR|UxRTJ4LkizJO69VS5? MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDNzM{OwO{c,OTh|MUOzNFc9N2F-
HepG2 NFfhXmtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXK3NkBp M3PyV2dzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NE4zOyEQvF2u MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDNzM{OwO{c,OTh|MUOzNFc9N2F-
K562 MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWW3NkBp NXL5Tm1PT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUzV4MjDj[YxteyCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVAvOjJizszNMi=> MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDNzM{OwO{c,OTh|MUOzNFc9N2F-
HepG2 M{f3T2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVG3NkBp NGDrOoJIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVAvOjFizszNMi=> MkexQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3MUK5PFQoRjF6NUGyPVg1RC:jPh?=
Huh7 M3j6TmN6fG:2b4jpZ4l1gSCjc4PhfS=> MoHnUm9XSVKWSWO6JGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIhmeGG2b3PlcIx2dGG{IHPhdoNqdm:vYTDj[YxtKGyrbnWgLGh2cDdrLDDDR|UxRTZwNkOg{txONg>? M1;MT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUe5O|g{Lz5zOEW3PVc5OzxxYU6=
HEK239 M4L4RWN6fG:2b4jpZ4l1gSCjc4PhfS=> MUO3NkBp NFmyXFFEgXSxdH;4bYNqfHliYXfhbY5{fCCKRVuyN|kh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTBwNEKg{txONg>? MljVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3NUiwOlAoRjJyNUW4NFYxRC:jPh?=
J774A1 NFHmZohEgXSxdH;4bYNqfHliYYPzZZk> NUHsWIdiS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhUjd5NFGxJINmdGy|IHL5JJRzgXCjbjDicJVmKGW6Y3z1d4lwdiCjc4PhfUwhUUN3ME2xNEDPxE1w NXPMT4hWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5N|Q3OzZpPkKyPVM1PjN4PD;hQi=>
KB3-1 M1[yXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlTtO|IhcHK| NXrKVmFPT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hU0J|LUGgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMkGg{txONg>? NHHTV3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEOxN|MxPyd-MUizNVM{ODd:L3G+
HepG2/Dox NFG3XplIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGLuZZM4OiCqcoO= NWnYOmF[T3Kxd4ToJIlvcGmkaYTpc44hd2ZibYXseIllenWpIILld4l{fGGwdDDoeY1idiCKZYDHNk9Fd3hiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFIxNjJizszNMi=> NV33V|ZTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1NVI6QDRpPkG4OVEzQTh2PD;hQi=>
HEK293 MnezR5l1d3SxeHnjbZR6KGG|c3H5 MYG3NkBpenN? MUjDfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KHKnc3H6eZJqdi2kYYPl[EBxdGG2ZTDy[YFl\XJiYX7hcJl{cXNuIFnDOVAhRSByLkO2NUDPxE1w NHe4PGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[1NVU{Pyd-Mk[2OVE2Ozd:L3G+
HEK293 Mn3YR5l1d3SxeHnjbZR6KGG|c3H5 MYS3NkBpenN? MUDDfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IHHsZY1ieiCkbIXlJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IECuN|UxPSEQvF2u M4XBRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5ME[wO|Y{Lz5{N{C2NFc3OzxxYU6=
A549 Mn3KR5l1d3SxeHnjbZR6KGG|c3H5 MWTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzMEBKSzVyIE2gNE43KM7:TT6= M17leFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEi5NlE1Lz5{N{C4PVIyPDxxYU6=
PC3 MofFR5l1d3SxeHnjbZR6KGG|c3H5 MUjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDQR|Mh[2WubIOsJGlEPTBiPTCwMlch|ryPLh?= M{W2WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEi5NlE1Lz5{N{C4PVIyPDxxYU6=
HEK293 NEfiN3pEgXSxdH;4bYNqfHliYYPzZZk> M3zCd2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KEiHS{K5N{Bk\WyuczDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3MDC9JFAvODByM{Wg{txONg>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzJzMkC3NEc,Ojd{MUKwO|A9N2F-
HEK293 NYSxOnpWT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFPqeHk4OiCqcoO= MVrHdo94fGhiaX7obYJqfGmxbjDv[kBJTUt{OUOgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KFC{ZYP0c2JtfWVic4ThbY5qdmdiYnHz[YQh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwJF0hOC5|NjFOwG0v MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDByMUC2O{c,OjhyMEGwOlc9N2F-
HEK293 MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{LNWmdzd3e2aDDpcohq[mm2aX;uJI9nKEiHS{K5N{Bk\WyuczygTWM2OCB;IECuN|k6KM7:TT6= MmfTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2MECyN|EoRjJ6NECwNlMyRC:jPh?=
SU-DHL10 MnHxR5l1d3SxeHnjbZR6KGG|c3H5 NV3DZnZzPzJiaILz M{PQNWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNWNUSKTEGwJINmdGy|IHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEeyJIhzeyCkeTDD[YxtXGm2cnWtS4xwKGy3bXnu[ZNk\W62IHHzd4F6NCCFQ{WwJF0hOC5zMTFOwG0v NWfI[451RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1NlMyODNpPkK4OVI{OTB|PD;hQi=>
MIAPaCa2 NHjKdYlEgXSxdH;4bYNqfHliYYPzZZk> NFf5ZXo4OiCqcoO= NGTuUZpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOUUGSYVPhNkBk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBi\nSncjC3NkBpenNiYomgR4VtdFSrdILlMWdtdyCudX3pcoV{[2WwdDDhd5NigSxiQ1O1NEA:KDBwMjFOwG0v NVLzZpQ{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1NlMyODNpPkK4OVI{OTB|PD;hQi=>
MDA-MB-231 NIjJR5ZEgXSxdH;4bYNqfHliYYPzZZk> M1H6eVczKGi{cx?= NUH1dnQ1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA4OiCqcoOgZpkhS2WubGTpeJJmNUeubzDseY1qdmW|Y3XueEBie3OjeTygR2M2OCB;IECuOFgh|ryPLh?= MlfKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3MkOxNFMoRjJ6NUKzNVA{RC:jPh?=
HEK293 MorPR5l1d3SxeHnjbZR6KGG|c3H5 M1O3VlczKGi{cx?= NGnX[mJEgXSxdH;4bYNqfHliYXfhbY5{fCCKRVuyPVMh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB5MjDodpMh[nliQXzhcYFzKGKudXWgZZN{[XluIFnDOVAhRSByLkS2JO69VS5? NVXwXYFmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki3O|Q1OjdpPkK4O|c1PDJ5PD;hQi=>
HL60 MljvRY51cXCjcnHzbZRq[yCjc4PhfS=> NH7VWHM4OiCqcoO= M4LDXGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFy2NEBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDFR|UxKD1iMD6wOVUh|ryPLh?= NFHOU3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEiwN|A1Pyd-Mki4NFMxPDd:L3G+
MOLT4 M{T5SmFvfGmyYYLhd4l1cWNiYYPzZZk> MVS3NkBpenN? M33l[2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTV;MWFQh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygSWM2OCB;IECuNVch|ryPLh?= NFzLfXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEiwN|A1Pyd-Mki4NFMxPDd:L3G+
Vero NGe5W3BEgXSxdH;4bYNqfHliYYPzZZk> NVzV[INsPzJiaILz MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBC\nKrY3HuJIdz\WWwIH3vcotmgSCYZYLvJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJBzd2yrZnXyZZRqd25iYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiQ1O1NEA:KDJwODFOwG0v MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDhyM{C0O{c,Ojh6MEOwOFc9N2F-
HEK293 NUP4UJdSS3m2b4TvfIlkcXS7IHHzd4F6 M2qxXVczKGi{cx?= M4rvUWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEiHS{K5N{Bk\WyuczDh[pRmeiB5MjDodpMh[nlicnXzZZp2emmwIHT5[UBj[XOnZDDhd5NigSxiSVO1NEA:KDBwNEWg{txONg>? MmnrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{M{[0PVIoRjJ7MkO2OFkzRC:jPh?=
HEK293T MnvuR5l1d3SxeHnjbZR6KGG|c3H5 NGXkNoM4OiCqcoO= M4\vN2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhGUzJ7M2SgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4h[2WubDD2bYFjcWyrdImgZYZ1\XJiN{KgbJJ{KGK7IFPlcIxVcXSncj3HcI8hemWjZ3XueEBj[XOnZDDseY1qdmW|Y3XuZ4Uh[XO|YYmsJGNEPTBiPTCwMlQzKM7:TT6= M{XzTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NE[5OVc2Lz5{OUS2PVU4PTxxYU6=
HCT8 MoHXRY51cXCjcnHzbZRq[yCjc4PhfS=> MofoOFghcHK| NI\3PVBCdnSrcHHyZZNqfGmlIHHjeIl3cXS7IHHnZYlve3RiQ4L5dJRwe3CxcnnkbZVuKHCjco\1cUBDT0ZiaX7m[YN1\WRiaX6gbJVu[W5iSFPUPEBk\WyuczDpcoN2[mG2ZXSg[o9zKDR6IHjyd{BjgSCISWTDM2RCWElic4ThbY5qdmdiYnHz[YQh\my3b4Lld4NmdmOnIHHzd4F6NCCHQ{WwJF0hOC53NTFOwG0v MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR4OUW3OUc,Ojl2Nkm1O|U9N2F-
HCT8 MXvBcpRqeGG{YYPpeIlkKGG|c3H5 NWq5cJNmPDhiaILz MYnBcpRqeGG{YYPpeIlkKGGldHn2bZR6KGGpYXnud5QhS3K7cITvd5BwemmmaYXtJJBien[3bTDTVGwhcW6oZXP0[YQhcW5iaIXtZY4hUEOWODDj[YxteyCrbnP1ZoF1\WRiZn;yJFQ5KGi{czDifUBHUVSFL1TBVGkhe3SjaX7pcoch[mG|ZXSg[ox2d3Knc3PlcoNmKGG|c3H5MEBGSzVyIE2gNE43KM7:TT6= NH;HUm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUS2PVU4PSd-Mkm0Olk2PzV:L3G+
HepG2 MWPDfZRwfG:6aXPpeJkh[XO|YYm= M3ryeVczKGi{cx?= M2G3U2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvR3zvJJJm[WenboSgZoF{\WRibIXtbY5me2OnbnPlJIF{e2G7LDDDR|UxKD1iMD64N{DPxE1w MmPQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2Nkm1O|UoRjJ7NE[5OVc2RC:jPh?=
HCT8 MmX3R5l1d3SxeHnjbZR6KGG|c3H5 M1HHfWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDhiY3XscJMtKEWFNUCgQUA3NjFizszNMi=> M{OxcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NE[5OVc2Lz5{OUS2PVU4PTxxYU6=
HEK293 NXe5XFFVS3m2b4TvfIlkcXS7IHHzd4F6 NYXGUFJLPzJiaILz M4rrWGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPlcIwh\3Kxd4ToJIFnfGW{IEeyJIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTygTWM2OCB;IECuOFYh|ryPLh?= NHHXW|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWzN|YyOSd-Mkm1N|M3OTF:L3G+
HEK293 M2j4[GN6fG:2b4jpZ4l1gSCjc4PhfS=> NHPhVpQ4OiCqcoO= NVLIWmNCS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEWNMkmzJINmdGy|IHHmeIVzKDd{IHjyd{BjgSC{ZYPhfpVzcW5iZInlJIJie2WmIH\seY9z\XOlZX7j[UBie3OjeTygTWM2OCB;IECuOEDPxE1w MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTl4OUK2Nkc,Ojl7NkmyOlI9N2F-
A549 M3y4e2N6fG:2b4jpZ4l1gSCjc4PhfS=> NYnQTo1xPDhiaILz NGjaV|dEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDR6IHjyd{whUUN3MDC9JFAvOyEQvF2u M3nFSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyNEG4O|Y{Lz5|MESxPFc3OzxxYU6=
PC3 M4XsO2N6fG:2b4jpZ4l1gSCjc4PhfS=> MUC0PEBpenN? MWjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDQR|Mh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|LDDJR|UxKD1iMD6zJO69VS5? NI\4[3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MESxPFc3Oyd-M{C0NVg4PjN:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cyclin D1 / CDK4; 

PubMed: 31022952     


(C) Effect of puromycin on cyclin D1 and CDK4 in a concentration-dependent manner in HCT116 and H1299 cells. Cells were treated with puromycin (0.25 or 0.5 μg/mL) for 24 h and subjected to Western blotting with antibodies of cyclin D1, CDK4 and β actin. (D) Effect of puromycin on cyclin D1 and CDK4 in a time-dependent manner in HCT116 and H1299 cells. Cells were treated with 0.5 μg/mL puromycin for 3, 6, 12 or 24 h and subjected to Western blotting with antibodies of cyclin D1, CDK4, and β-actin.

PARP / Bcl-xL / Bcl-2 / p-Akt / Akt; 

PubMed: 31022952     


(E) Effect of puromycin on Poly (ADP-ribose) polymerase (PARP), Bcl-xL, Bcl-2, AKT, and p-AKT (v-akt murine thymoma viral oncogene homolog) in a concentration-dependent manner in HCT116 and H1299 cells. Cells were treated with puromycin (0.25 or 0.5 μg/mL) for 24 h and subjected to Western blotting with antibodies of PARP, Bcl-xL, Bcl-2, p-AKT, AKT, and β-actin. (F) Effect of puromycin on PARP, Bcl-xL, Bcl-2, AKT, and p-AKT in a time-dependent manner in HCT116 and H1299 cells. Cells were treated with 0.5 μg/mL of puromycin for 3, 6, 12 or 24 h and subjected to Western blotting with antibodies of PARP, Bcl-xL, Bcl-2, p-AKT, AKT, and β-actin.

p53 / p21 / c-Myc; 

PubMed: 31022952     


Effect of puromycin on p53, p21, and c-Myc in a concentration and time-dependent manner in HCT116 cells. HCT116 cells were exposed to various concentrations of puromycin (0.25 or 0.5 μg/mL) for 24 h and then subjected to Western blotting with antibodies of p53, p21, and c-Myc. Also, HCT116 cells were treated with 0.5 μg/mL puromycin for 6, 12, or 24 h and subjected to Western blotting.

31022952
Growth inhibition assay
Cell viability; 

PubMed: 31022952     


Cytotoxicity of puromycin in HCT116, SW620, HCT15, and H1299 cells in a concentration-dependent manner by 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Data represent means ± SD. *, p < 0.05, **, p < 0.01, ***, p< 0.001 vs. untreated control.

31022952

Protocol

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (183.67 mM)
Water 100 mg/mL warmed (183.67 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 544.43
Formula

C22H29N7O5.2HCl

CAS No. 58-58-2
Storage powder
in solvent
Synonyms CL13900
Smiles CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl

In vivo Formulation Calculator (Clear solution)

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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

  • C1=C0/X C1: LOG(C1):
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    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID