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Puromycin 2HCl

For research use only.

Catalog No.S7417

57 publications

Puromycin 2HCl Chemical Structure

CAS No. 58-58-2

Puromycin 2HCl is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.

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Selleck's Puromycin 2HCl has been cited by 57 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Puromycin 2HCl is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.
In vitro

The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. [1] Puromycin can be used for the selection of recombinant cells from noncultured cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K562 M3zYWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoHaO|IhcA>? NGrxcnFIem:5dHigbY5pcWKrdHnvckBw\iCmb4jvdpVjcWOrbj3y[ZNqe3SjboSgbJVu[W5iS{W2NkBk\WyuczDh[pRmeiB5MjDodpMh[nliU2LCJIF{e2G7LDDJR|UxRTJ4LkizJO69VS5? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDNzM{OwO{c,OTh|MUOzNFc9N2F-
HepG2 NYO0fI5pT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1PwbFczKGh? NELJTGxIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvOjNizszNMi=> NUHSWHljRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizNVM{ODdpPkG4N|E{OzB5PD;hQi=>
K562 NGTXbHZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXPL[ld1PzJiaB?= M172[2dzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGs2PjJiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlIzKM7:TT6= MoDoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|MUOzNFcoRjF6M{GzN|A4RC:jPh?=
HepG2 MYLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUL4SnpCPzJiaB?= NES2[3pIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVAvOjFizszNMi=> M4rKclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUGyPVg1Lz5zOEWxNlk5PDxxYU6=
Huh7 Mn7BR5l1d3SxeHnjbZR6KGG|c3H5 M{DtcG5QXkGUVFnTPkBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBp\XCjdH;j[YxtfWyjcjDjZZJkcW6xbXGgZ4VtdCCuaX7lJEhJfWh5KTygR2M2OD14Lk[zJO69VS5? NWPEbndCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1O|k4QDNpPkG4OVc6Pzh|PD;hQi=>
HEK239 M2XPbGN6fG:2b4jpZ4l1gSCjc4PhfS=> NGfOVHQ4OiCq MYPDfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{M{mgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KEGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwQVAvPDJizszNMi=> MnXyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3NUiwOlAoRjJyNUW4NFYxRC:jPh?=
J774A1 MkG4R5l1d3SxeHnjbZR6KGG|c3H5 MVnDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDKO|c1STFiY3XscJMh[nlidIL5dIFvKGKudXWg[ZhkdHW|aX;uJIF{e2G7LDDJR|UxRTFyIN88UU4> NHT4O2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmzOFY{Pid-MkK5N|Q3OzZ:L3G+
KB3-1 NH7rbFBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mm[zO|IhcHK| MXHHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDLRlMuOSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4zOSEQvF2u NYi5ZYR[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizNVM{ODdpPkG4N|E{OzB5PD;hQi=>
HepG2/Dox MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWLjNXB3PzJiaILz NXXBe4ozT3Kxd4ToJIlvcGmkaYTpc44hd2ZibYXseIllenWpIILld4l{fGGwdDDoeY1idiCKZYDHNk9Fd3hiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFIxNjJizszNMi=> M1XJflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUGyPVg1Lz5zOEWxNlk5PDxxYU6=
HEK293 M3HQdWN6fG:2b4jpZ4l1gSCjc4PhfS=> NIPrfJA4OiCqcoO= NIrKclhEgXSxdH;4bYNqfHliYXfhbY5{fCCKRVuyPVMh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiN{KgbJJ{KGK7IILld4F7fXKrbj3iZZNm\CCybHH0[UBz\WGmZYKgZY5idHm|aYOsJGlEPTBiPTCwMlM3OSEQvF2u NGnP[Wc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[1NVU{Pyd-Mk[2OVE2Ozd:L3G+
HEK293 NUjS[m9CS3m2b4TvfIlkcXS7IHHzd4F6 NU\id5FEPzJiaILz MYXDfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IHHsZY1ieiCkbIXlJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IECuN|UxPSEQvF2u M37nNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5ME[wO|Y{Lz5{N{C2NFc3OzxxYU6=
A549 NHzvXFlEgXSxdH;4bYNqfHliYYPzZZk> NWDa[o55S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyxiSVO1NEA:KDBwNjFOwG0v NF2yU|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C4PVIyPCd-MkewPFkzOTR:L3G+
PC3 MYfDfZRwfG:6aXPpeJkh[XO|YYm= M1LEZ2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBEOyClZXzsd{whUUN3MDC9JFAvPyEQvF2u NIjj[Xk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C4PVIyPCd-MkewPFkzOTR:L3G+
HEK293 MkLDR5l1d3SxeHnjbZR6KGG|c3H5 M{fFPGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEiHS{K5N{Bk\WyuczDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3MDC9JFAvODByM{Wg{txONg>? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzJzMkC3NEc,Ojd{MUKwO|A9N2F-
HEK293 M1rVV2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2rMU|czKGi{cx?= NF\iNppIem:5dHigbY5pcWKrdHnvckBw\iCKRVuyPVMh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IGDy[ZN1d0KudXWgd5RicW6rbnegZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxKD1iMD6zOkDPxE1w MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDByMUC2O{c,OjhyMEGwOlc9N2F-
HEK293 Mlm0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGXMblBIem:5dHigbY5pcWKrdHnvckBw\iCKRVuyPVMh[2WubIOsJGlEPTBiPTCwMlM6QSEQvF2u NV;tVnhrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0NFAzOzFpPkK4OFAxOjNzPD;hQi=>
SU-DHL10 NIf2WXREgXSxdH;4bYNqfHliYYPzZZk> MUO3NkBpenN? MUDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTWU1FUExzMDDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB5MjDodpMh[nliQ3XscHRqfHKnLVfsc{BtfW2rbnXzZ4VvfCCjc4PhfUwhS0N3MDC9JFAvOTFizszNMi=> MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV{M{GwN{c,Ojh3MkOxNFM9N2F-
MIAPaCa2 MVHDfZRwfG:6aXPpeJkh[XO|YYm= MoHwO|IhcHK| Ml;oR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWlCWGGFYUKgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IFPlcIxVcXS{ZT3HcI8hdHWvaX7ld4NmdnRiYYPzZZktKEOFNUCgQUAxNjJizszNMi=> NIPYPZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEWyN|ExOyd-Mki1NlMyODN:L3G+
MDA-MB-231 NF3PUGpEgXSxdH;4bYNqfHliYYPzZZk> Mny5O|IhcHK| NEjMe3lEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIF{e2W|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFczKGi{czDifUBE\WyuVHn0doUuT2yxIHz1cYlv\XOlZX70JIF{e2G7LDDDR|UxKD1iMD60PEDPxE1w MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV{M{GwN{c,Ojh3MkOxNFM9N2F-
HEK293 MlntR5l1d3SxeHnjbZR6KGG|c3H5 NG[3fG04OiCqcoO= MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA4OiCqcoOgZpkhSWyjbXHyJIJtfWViYYPzZZktKEmFNUCgQUAxNjR4IN88UU4> MnvPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5N{S0NlcoRjJ6N{e0OFI4RC:jPh?=
HL60 MV7BcpRqeGG{YYPpeIlkKGG|c3H5 NVzBUo9HPzJiaILz Mn6wRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKTE[wJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFXDOVAhRSByLkC1OUDPxE1w M2Hj[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OECzNFQ4Lz5{OEiwN|A1PzxxYU6=
MOLT4 MmDjRY51cXCjcnHzbZRq[yCjc4PhfS=> NIPRUWk4OiCqcoO= MWfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2RTGS0JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFXDOVAhRSByLkG3JO69VS5? Mo\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6MEOwOFcoRjJ6OECzNFQ4RC:jPh?=
Vero M1nadmN6fG:2b4jpZ4l1gSCjc4PhfS=> NIjSV3M4OiCqcoO= MXXDfZRwfG:6aXPpeJkh[WejaX7zeEBC\nKrY3HuJIdz\WWwIH3vcotmgSCYZYLvJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJBzd2yrZnXyZZRqd25iYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiQ1O1NEA:KDJwODFOwG0v NWXwepBVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki4NFMxPDdpPkK4PFA{ODR5PD;hQi=>
HEK293 NVnQOm1MS3m2b4TvfIlkcXS7IHHzd4F6 NEHXVWs4OiCqcoO= Ml3yR5l1d3SxeHnjbZR6KGGpYXnud5QhUEWNMkmzJINmdGy|IHHmeIVzKDd{IHjyd{BjgSC{ZYPhfpVzcW5iZInlJIJie2WmIHHzd4F6NCCLQ{WwJF0hOC52NTFOwG0v NYnSTYFNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyN|Y1QTJpPkK5NlM3PDl{PD;hQi=>
HEK293T MXPDfZRwfG:6aXPpeJkh[XO|YYm= NYjuNGtDPzJiaILz MWnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDISWszQTOWIHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvR3zvJJJm[WenboSgZoF{\WRibIXtbY5me2OnbnPlJIF{e2G7LDDDR|UxKD1iMD60NkDPxE1w MlvrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2Nkm1O|UoRjJ7NE[5OVc2RC:jPh?=
HCT8 NEPZO4dCdnSrcHHyZZNqfGmlIHHzd4F6 NFS2dHQ1QCCqcoO= MmPORY51cXCjcnHzbZRq[yCjY4Tpeol1gSCjZ3HpcpN1KEO{eYD0c5Nxd3KrZHn1cUBx[XK4dX2gRmdHKGmwZnXjeIVlKGmwIHj1cYFvKEiFVEigZ4VtdHNiaX7jeYJifGWmIH\vdkA1QCCqcoOgZpkhTkmWQz;ERXBKKHO2YXnubY5oKGKjc3XkJIZtfW:{ZYPj[Y5k\SCjc4PhfUwhTUN3MDC9JFAvPTVizszNMi=> NYPFWpNDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0Olk2PzVpPkK5OFY6PTd3PD;hQi=>
HCT8 NUXZc5FESW62aYDhdoF{cXSrYzDhd5NigQ>? NYDpUmZYPDhiaILz Ml:xRY51cXCjcnHzbZRq[yCjY4Tpeol1gSCjZ3HpcpN1KEO{eYD0c5Nxd3KrZHn1cUBx[XK4dX2gV3BNKGmwZnXjeIVlKGmwIHj1cYFvKEiFVEigZ4VtdHNiaX7jeYJifGWmIH\vdkA1QCCqcoOgZpkhTkmWQz;ERXBKKHO2YXnubY5oKGKjc3XkJIZtfW:{ZYPj[Y5k\SCjc4PhfUwhTUN3MDC9JFAvPiEQvF2u MofWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2Nkm1O|UoRjJ7NE[5OVc2RC:jPh?=
HepG2 MULDfZRwfG:6aXPpeJkh[XO|YYm= M17ueFczKGi{cx?= M2\6Z2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvR3zvJJJm[WenboSgZoF{\WRibIXtbY5me2OnbnPlJIF{e2G7LDDDR|UxKD1iMD64N{DPxE1w Mn3lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2Nkm1O|UoRjJ7NE[5OVc2RC:jPh?=
HCT8 NIPLeW1EgXSxdH;4bYNqfHliYYPzZZk> NVGzN4tuS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWODDj[YxteyxiRVO1NEA:KDZwMTFOwG0v M2OxdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NE[5OVc2Lz5{OUS2PVU4PTxxYU6=
HEK293 M2PPfGN6fG:2b4jpZ4l1gSCjc4PhfS=> MkT5O|IhcHK| NFe1cZREgXSxdH;4bYNqfHliYXfhbY5{fCCKRVuyPVMh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKGe{b4f0bEBi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTBiPTCwMlQ3KM7:TT6= NXfzSYliRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1N|M3OTFpPkK5OVM{PjFzPD;hQi=>
HEK293 MoS1R5l1d3SxeHnjbZR6KGG|c3H5 NWTvUWZoPzJiaILz NFHKWodEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJTUt{OUOgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KHKnc3H6eZJqdiCmeXWgZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxKD1iMD60JO69VS5? NYHteItiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm5OlkzPjJpPkK5PVY6OjZ{PD;hQi=>
A549 M{S5VmN6fG:2b4jpZ4l1gSCjc4PhfS=> MWe0PEBpenN? Mn3IR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOsJGlEPTBiPTCwMlMh|ryPLh?= NWPabmhlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C0NVg4PjNpPkOwOFE5PzZ|PD;hQi=>
PC3 MV7DfZRwfG:6aXPpeJkh[XO|YYm= MYO0PEBpenN? MWjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDQR|Mh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|LDDJR|UxKD1iMD6zJO69VS5? NGLFcG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MESxPFc3Oyd-M{C0NVg4PjN:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cyclin D1 / CDK4; 

PubMed: 31022952     


(C) Effect of puromycin on cyclin D1 and CDK4 in a concentration-dependent manner in HCT116 and H1299 cells. Cells were treated with puromycin (0.25 or 0.5 μg/mL) for 24 h and subjected to Western blotting with antibodies of cyclin D1, CDK4 and β actin. (D) Effect of puromycin on cyclin D1 and CDK4 in a time-dependent manner in HCT116 and H1299 cells. Cells were treated with 0.5 μg/mL puromycin for 3, 6, 12 or 24 h and subjected to Western blotting with antibodies of cyclin D1, CDK4, and β-actin.

PARP / Bcl-xL / Bcl-2 / p-Akt / Akt; 

PubMed: 31022952     


(E) Effect of puromycin on Poly (ADP-ribose) polymerase (PARP), Bcl-xL, Bcl-2, AKT, and p-AKT (v-akt murine thymoma viral oncogene homolog) in a concentration-dependent manner in HCT116 and H1299 cells. Cells were treated with puromycin (0.25 or 0.5 μg/mL) for 24 h and subjected to Western blotting with antibodies of PARP, Bcl-xL, Bcl-2, p-AKT, AKT, and β-actin. (F) Effect of puromycin on PARP, Bcl-xL, Bcl-2, AKT, and p-AKT in a time-dependent manner in HCT116 and H1299 cells. Cells were treated with 0.5 μg/mL of puromycin for 3, 6, 12 or 24 h and subjected to Western blotting with antibodies of PARP, Bcl-xL, Bcl-2, p-AKT, AKT, and β-actin.

p53 / p21 / c-Myc; 

PubMed: 31022952     


Effect of puromycin on p53, p21, and c-Myc in a concentration and time-dependent manner in HCT116 cells. HCT116 cells were exposed to various concentrations of puromycin (0.25 or 0.5 μg/mL) for 24 h and then subjected to Western blotting with antibodies of p53, p21, and c-Myc. Also, HCT116 cells were treated with 0.5 μg/mL puromycin for 6, 12, or 24 h and subjected to Western blotting.

31022952
Growth inhibition assay
Cell viability; 

PubMed: 31022952     


Cytotoxicity of puromycin in HCT116, SW620, HCT15, and H1299 cells in a concentration-dependent manner by 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Data represent means ± SD. *, p < 0.05, **, p < 0.01, ***, p< 0.001 vs. untreated control.

31022952

Protocol

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (183.67 mM)
Water 100 mg/mL warmed (183.67 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 544.43
Formula

C22H29N7O5.2HCl

CAS No. 58-58-2
Storage powder
in solvent
Synonyms N/A
Smiles CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl

In vivo Formulation Calculator (Clear solution)

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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

  • C1=C0/X C1: LOG(C1):
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    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID