Puromycin 2HCl

Catalog No.S7417

Puromycin 2HCl Chemical Structure

Molecular Weight(MW): 544.43

Puromycin 2HCl is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.

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Cited by 14 publications

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Biological Activity

Description Puromycin 2HCl is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.
In vitro

The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. [1] Puromycin can be used for the selection of recombinant cells from noncultured cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HepG2 cells M3LZbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXrOTppoPzJiaB?= NHv5N3BIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVAvOjFizszNMi=> NEj1O5QyQDVzMkm4OC=>
human K562 cells NFXyRmVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIK3XoI4OiCq M13QVmdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGs2PjJiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlIzKM7:TT6= NFXTOI4yQDNzM{OwOy=>
human HepG2 cells NYfYeJl4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NH;lUoo4OiCq MnHBS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlI{KM7:TT6= MlPzNVg{OTN|MEe=
HEK239 cells MmTTR5l1d3SxeHnjxsBie3OjeR?= NHj1NoI4OiCq NYexNYVtS3m2b4TvfIlkcXS7IHHnZYlve3RiSFXLNlM6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDBcIFu[XJiYnz1[UBie3OjeTygTWM2OD1yLkSyJO69VS5? NWLqWHNMOjB3NUiwOlA>
human hepatocellular carcinoma cell line (Huh7) MYHDfZRwfG:6aXRCpIF{e2G7 NYDPe3k1Vk:YQWLUTXM7KEO7dH;0c5hq[2m2eTDh[4FqdnO2IHj1cYFvKGincHH0c4NmdGy3bHHyJINiemOrbn;tZUBk\WyuIHzpcoUhMEi3aEepMEBESzVyPU[uOlMh|ryPLh?= Mlf3NVg2Pzl5OEO=
mouse J774A1 cells NX21UpBLS3m2b4TvfIlkyqCjc4PhfS=> MlrtR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgTlc4PEFzIHPlcIx{KGK7IITyfZBidiCkbIXlJIV5[2y3c3nvckBie3OjeTygTWM2OD1zMDFOwG0v MoTVNlI6OzR4M{[=
human K562 cells MkXYS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVq3NkBp NG\FfGZIem:5dHigbY5pcWKrdHnvckBw\iCmb4jvdpVjcWOrbj3y[ZNqe3SjboSgbJVu[W5iS{W2NkBk\WyuczDh[pRmeiB5MjDodpMh[nliU2LCJIF{e2G7LDDJR|UxRTJ4LkizJO69VS5? MoXpNVg{OTN|MEe=

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot
Cyclin D1 / CDK4; 

PubMed: 31022952     


(C) Effect of puromycin on cyclin D1 and CDK4 in a concentration-dependent manner in HCT116 and H1299 cells. Cells were treated with puromycin (0.25 or 0.5 μg/mL) for 24 h and subjected to Western blotting with antibodies of cyclin D1, CDK4 and β actin. (D) Effect of puromycin on cyclin D1 and CDK4 in a time-dependent manner in HCT116 and H1299 cells. Cells were treated with 0.5 μg/mL puromycin for 3, 6, 12 or 24 h and subjected to Western blotting with antibodies of cyclin D1, CDK4, and β-actin.

PARP / Bcl-xL / Bcl-2 / p-Akt / Akt; 

PubMed: 31022952     


(E) Effect of puromycin on Poly (ADP-ribose) polymerase (PARP), Bcl-xL, Bcl-2, AKT, and p-AKT (v-akt murine thymoma viral oncogene homolog) in a concentration-dependent manner in HCT116 and H1299 cells. Cells were treated with puromycin (0.25 or 0.5 μg/mL) for 24 h and subjected to Western blotting with antibodies of PARP, Bcl-xL, Bcl-2, p-AKT, AKT, and β-actin. (F) Effect of puromycin on PARP, Bcl-xL, Bcl-2, AKT, and p-AKT in a time-dependent manner in HCT116 and H1299 cells. Cells were treated with 0.5 μg/mL of puromycin for 3, 6, 12 or 24 h and subjected to Western blotting with antibodies of PARP, Bcl-xL, Bcl-2, p-AKT, AKT, and β-actin.

p53 / p21 / c-Myc; 

PubMed: 31022952     


Effect of puromycin on p53, p21, and c-Myc in a concentration and time-dependent manner in HCT116 cells. HCT116 cells were exposed to various concentrations of puromycin (0.25 or 0.5 μg/mL) for 24 h and then subjected to Western blotting with antibodies of p53, p21, and c-Myc. Also, HCT116 cells were treated with 0.5 μg/mL puromycin for 6, 12, or 24 h and subjected to Western blotting.

31022952
Growth inhibition assay
Cell viability; 

PubMed: 31022952     


Cytotoxicity of puromycin in HCT116, SW620, HCT15, and H1299 cells in a concentration-dependent manner by 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Data represent means ± SD. *, p < 0.05, **, p < 0.01, ***, p< 0.001 vs. untreated control.

31022952

Protocol

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (183.67 mM)
Water 100 mg/mL warmed (183.67 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 544.43
Formula

C22H29N7O5.2HCl
CAS No. 58-58-2
Storage powder
in solvent
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID