Puromycin 2HCl

For research use only.

Catalog No.S7417

27 publications

Molecular Weight(MW): 544.43

Puromycin 2HCl is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.

Selleck's Puromycin 2HCl has been cited by 27 publications

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Biological Activity

Description

Puromycin 2HCl is an aminonucleoside antibiotic, which acts as a protein synthesis inhibitor.

In vitro

The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. ^[1] Puromycin can be used for the selection of recombinant cells from noncultured cells. ^[2]

Cell Data

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
human HepG2 cells	NIPNVmxIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	NX7sdotmPzJiaB?=	MmLsS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgO\|IhcHK\|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlIyKM7:TT6=	NVvzRmdpOTh3MUK5PFQ>
human K562 cells	MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	NX[zPFdLPzJiaB?=	MnK5S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gT\|U3OiClZXzsd{Bi\nSncjC3NkBpenNiYomgV3JDKGG\|c3H5MEBKSzVyPUCuNlIh\|ryPLh?=	NX7HO4ZVOTh\|MUOzNFc>
human HepG2 cells	NF;uTWRIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	M{\5clczKGh?	NHnEXJZIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvOjNizszNMi=>	MXmxPFMyOzNyNx?=
HEK239 cells	MnTTR5l1d3SxeHnjxsBie3OjeR?=	NX3PcZNpPzJiaB?=	MkPGR5l1d3SxeHnjbZR6KGGpYXnud5QhUEWNMkO5JINmdGy\|IHHmeIVzKDd{IHjyd{BjgSCDbHHtZZIh[my3ZTDhd5NigSxiSVO1NF0xNjR{IN88UU4>	NXnQ[VlyOjB3NUiwOlA>
human hepatocellular carcinoma cell line (Huh7)	Mkf5R5l1d3SxeHnjxsBie3OjeR?=		M4jRRW5QXkGUVFnTPkBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBp\XCjdH;j[YxtfWyjcjDjZZJkcW6xbXGgZ4VtdCCuaX7lJEhJfWh5KTygR2M2OD14Lk[zJO69VS5?	MWGxPFU4QTd6Mx?=
mouse J774A1 cells	NWHEXIlnS3m2b4TvfIlkyqCjc4PhfS=>		NGPxblNEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBLPzd2QUGgZ4VtdHNiYomgeJJ6eGGwIHLseYUh\XilbIXzbY9vKGG\|c3H5MEBKSzVyPUGwJO69VS5?	M1HJ[\|IzQTN2NkO2
human K562 cells	M2jFdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	NVGzPXJQPzJiaB?=	NUXwfJpxT3Kxd4ToJIlvcGmkaYTpc44hd2ZiZH;4c5J2[mmlaX6tdoV{cXO2YX70JIh2dWGwIFu1OlIh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IGPSRkBie3OjeTygTWM2OD1{Nj64N{DPxE1w	NITQZ48yQDNzM{OwOy=>

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Cyclin D1 / CDK4; PubMed: 31022952 Cancers (Basel) (C) Effect of puromycin on cyclin D1 and CDK4 in a concentration-dependent manner in HCT116 and H1299 cells. Cells were treated with puromycin (0.25 or 0.5 μg/mL) for 24 h and subjected to Western blotting with antibodies of cyclin D1, CDK4 and β actin. (D) Effect of puromycin on cyclin D1 and CDK4 in a time-dependent manner in HCT116 and H1299 cells. Cells were treated with 0.5 μg/mL puromycin for 3, 6, 12 or 24 h and subjected to Western blotting with antibodies of cyclin D1, CDK4, and β-actin. PARP / Bcl-xL / Bcl-2 / p-Akt / Akt; PubMed: 31022952 Cancers (Basel) (E) Effect of puromycin on Poly (ADP-ribose) polymerase (PARP), Bcl-xL, Bcl-2, AKT, and p-AKT (v-akt murine thymoma viral oncogene homolog) in a concentration-dependent manner in HCT116 and H1299 cells. Cells were treated with puromycin (0.25 or 0.5 μg/mL) for 24 h and subjected to Western blotting with antibodies of PARP, Bcl-xL, Bcl-2, p-AKT, AKT, and β-actin. (F) Effect of puromycin on PARP, Bcl-xL, Bcl-2, AKT, and p-AKT in a time-dependent manner in HCT116 and H1299 cells. Cells were treated with 0.5 μg/mL of puromycin for 3, 6, 12 or 24 h and subjected to Western blotting with antibodies of PARP, Bcl-xL, Bcl-2, p-AKT, AKT, and β-actin. p53 / p21 / c-Myc; PubMed: 31022952 Cancers (Basel) Effect of puromycin on p53, p21, and c-Myc in a concentration and time-dependent manner in HCT116 cells. HCT116 cells were exposed to various concentrations of puromycin (0.25 or 0.5 μg/mL) for 24 h and then subjected to Western blotting with antibodies of p53, p21, and c-Myc. Also, HCT116 cells were treated with 0.5 μg/mL puromycin for 6, 12, or 24 h and subjected to Western blotting.	31022952
Growth inhibition assay	Cell viability; PubMed: 31022952 Cancers (Basel) Cytotoxicity of puromycin in HCT116, SW620, HCT15, and H1299 cells in a concentration-dependent manner by 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Data represent means ± SD. , p < 0.05, , p < 0.01, **, p< 0.001 vs. untreated control.	31022952

Protocol

References

Solubility (25°C)

In vitro	DMSO	100 mg/mL warmed (183.67 mM)
	Water	100 mg/mL warmed (183.67 mM)
	Ethanol	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Puromycin 2HCl SDF

Molecular Weight	544.43
Formula	C₂₂H₂₉N₇O₅.2HCl
CAS No.	58-58-2
Storage	3 years -20°C powder
Storage	2 years -80°C in solvent
Synonyms	N/A
Smiles	Cl.Cl.COC1=CC=C(CC(N)C(=O)NC2C(O)C(OC2CO)[N]3C=NC4=C3N=CN=C4N(C)C)C=C1

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Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Puromycin 2HCl