Oligomycin A

Catalog No.S1478 Synonyms: MCH 32

For research use only.

Oligomycin A (MCH 32) is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.

Oligomycin A Chemical Structure

CAS No. 579-13-5

Selleck's Oligomycin A has been cited by 48 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Oligomycin A (MCH 32) is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.
Targets
ATP synthase [1]
(Cell-free assay)
In vitro

Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.Oligomycin A inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. The inhibition of ATP synthesis by oligomycin A will significantly reduce electron flow through the electron transport chain; however, electron flow is not stopped completely due to a process known as proton leak or mitochondrial uncoupling. [1] In a group of cancer cells, Oligomycin at 100 ng/ml completely inhibits oxidative phosphorylation activity in 1 h and induces various levels of glycolysis gains by 6 h. [2] Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis. [3] Oligomycin inhibits mitochondrial respiration in melanoma, sensitizes melanoma cells to therapy, blocks the emergence of the slow-cycling, long-term tumor-maintaining melanoma cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 cells NYTleWZOTnWwY4Tpc44h[XO|YYm= M1jhU|I2NCB3MDygO|UtKDFyMDFOwG0> NUKwVlU{\WyndnH0[YQhfGinIHfseYNwe2VidYD0ZYtmKGyndnXsJIJ2fCCwb4Sg[I9{\S2mZYDlcoRmdnR? NYrXSowyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyNFU2PzJpPkOwNlA2PTd{PD;hQi=>
Vero cells MXHGeY5kfGmxbjDhd5NigQ>? MmPZNE4xOSxiMD6xMEAyNCCjbnSgNVAh|ryP MV\hJIRm[3KnYYPlJIlvKHSqZTD2bYFjcWyrdImgc4YhXmW{bzDj[YxteyBqfkKwKUBz\WS3Y4Tpc44qKGG2IHnuZ5Jm[XOrbnegZ49v[2WwdILheIlwdnNw M{\YV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OEW5O|Q2Lz5{Nki1PVc1PTxxYU6=
ISE6 cells NHnWe3ZHfW6ldHnvckBie3OjeR?= MkT6NE4xOSxiMD6xMEAyNCCjbnSgNVAh|ryP NUT6ZWhJ[SCmZXPy[YF{\SCrbjD0bIUhfmmjYnnsbZR6KG:oIFnTSVYh[2WubIOgLJ43OCVrIHH0JIlv[3KnYYPpcoch[2:wY3XueJJifGmxboOu NIr3eWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nki1PVc1PSd-Mk[4OVk4PDV:L3G+
Assay
Methods Test Index PMID
Western blot p-GCN2 / GCN2 / p-eIF2α / eIF2α / ATF4 / xCT ; pAkt / Her2 / Raf / Hsp70 / Hsp90 ; p-T389 S6K1 / S6K1 / p-T172 AMPK / AMPK ; Cox1p / Cox2p / Cox3p / Cox4p 27708226 24450340 20444419 19735676

Protocol (from reference)

Cell Research:[2]
  • Cell lines: H1229
  • Concentrations: 100 ng/ml
  • Incubation Time: 1 h
  • Method: ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 10 mg/mL
(12.64 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.

15 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 791.06
Formula

C45H74O11

CAS No. 579-13-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC1CCC2C(C(C(C3(O2)CCC(C(O3)CC(C)O)C)C)OC(=O)C=CC(C(C(C(=O)C(C(C(C(=O)C(C(C(CC=CC=C1)C)O)(C)O)C)O)C)C)O)C)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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