Oligomycin A

Catalog No.S1478 Synonyms: MCH 32

Oligomycin A Chemical Structure

Molecular Weight(MW): 791.06

Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.

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Cited by 10 Publications

3 Customer Reviews

  • (F) Detection of expression of SDHA and TOMM20 by immunoblotting in wild-type and Atg7-/- K562 cells with indicated drugs. The expression levels were quantified with number indicated. (G) Quantification analysis of mitochondrial mass in wild-type and Atg7-/- K562 cells when cultured with oligomycin(100 ng/ml), antimycin A(50 μM) and Baf-A1(10 nM).

    Sci Rep, 2016, 6:24641.. Oligomycin A purchased from Selleck.

    Western blot analysis of CDK2, CDK4, CDK6, p21 and p27 protein expression levels in SW480 and SW620 treated with Oligo A (1 μM) for 0-48 h; β-actin was used as a loading control.

    Int J Oncol, 2018, 53(6):2590-2604. Oligomycin A purchased from Selleck.

  • (A) Replication of the H6N2 and H7N1 AIV was measured in presence or absence of 125μμM oligomycin. CLEC213 cells were infected at an MOI of 1. Total RNA was extracted 16 h pi and segment 7 vRNA was quantified by qRT-PCR. Results are expressed as copy numbers per μμg of total RNA, *: p-value<0.05, ***: p-value<0.001. (B) Minigenome activity in H6N2-infected cells in the presence or absence of oligomycin (125μμM). Results are expressed as a ratio of RLU on β-Gal activity ± SEM, ***: p-value<0.001.

    PLoS One, 2017, 12(4):e0176355. Oligomycin A purchased from Selleck.

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Biological Activity

Description Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.
Targets
ATP synthase [1]
(Cell-free assay)
In vitro

Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.Oligomycin A inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. The inhibition of ATP synthesis by oligomycin A will significantly reduce electron flow through the electron transport chain; however, electron flow is not stopped completely due to a process known as proton leak or mitochondrial uncoupling. [1] In a group of cancer cells, Oligomycin at 100 ng/ml completely inhibits oxidative phosphorylation activity in 1 h and induces various levels of glycolysis gains by 6 h. [2] Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis. [3] Oligomycin inhibits mitochondrial respiration in melanoma, sensitizes melanoma cells to therapy, blocks the emergence of the slow-cycling, long-term tumor-maintaining melanoma cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 cells M{frdWZ2dmO2aX;uJIF{e2G7 NIqzb40zPSxiNUCsJFc2NCBzMECg{txO M2\oOIVt\X[jdHXkJJRp\SCpbIXjc5NmKHWydHHr[UBt\X[nbDDieZQhdm:2IHTvd4Uu\GWyZX7k[Y51 NGTOOZE{ODJyNUW3Ni=>
Vero cells M3Ti[GZ2dmO2aX;uJIF{e2G7 MmPVNE4xOSxiMD6xMEAyNCCjbnSgNVAh|ryP MnHUZUBl\WO{ZXHz[UBqdiC2aHWgeoli[mmuaYT5JI9nKF[ncn:gZ4VtdHNiKI6yNEUhemWmdXP0bY9vMSCjdDDpcoNz\WG|aX7nJINwdmOnboTyZZRqd26|Lh?= MUWyOlg2QTd2NR?=
ISE6 cells NIG0NGtHfW6ldHnvckBie3OjeR?= M1e2[VAvODFuIECuNUwhOSxiYX7kJFExKM7:TR?= M1;Dc4Eh\GWlcnXhd4UhcW5idHjlJJZq[WKrbHn0fUBw\iCLU1W2JINmdGy|IDj+OlAmMSCjdDDpcoNz\WG|aX7nJINwdmOnboTyZZRqd26|Lh?= M{GxWlI3QDV7N{S1

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-GCN2 / GCN2 / p-eIF2α / eIF2α / ATF4 / xCT; 

PubMed: 27708226     


Western blot analysis of the GCN2-eIF2α-ATF4-xCT pathway in the SC-M1 cells under oligomycin (O, 1 μg/ml) and N-acetyl cysteine (NAC, N, 5 mM) treatments for 24 h. The immunoblot values were normalized to α-tubulin.

pAkt / Her2 / Raf / Hsp70 / Hsp90 ; 

PubMed: 24450340     


Western blot for Hsp90 client proteins (pAkt, Her2, and Raf) and Hsp70, Hsp90, and F1F0 ATP synthase using MCF7 cell lysates after 24 or 48 h of incubation with the indicated amount of vehicle (0.25% DMSO) or oligomycin A. The calculated EC50 value of oligomycin A against the MCF7 cell line was 7.35 μM.

p-T389 S6K1 / S6K1 / p-T172 AMPK / AMPK ; 

PubMed: 20444419     


TSC2+/+MEFs were treated with either 10μM oligomycin at the indicated times. * denotes the addition of a fresh aliquot of either ATP-depleting agent following a 60min incubation with the initial inhibitor. The data shown are representative of at least three independent experiments.

Cox1p / Cox2p / Cox3p / Cox4p ; 

PubMed: 19735676     


Western-blot analyses of the steady state concentrations of several COX and ATPase subunits (indicated in the margin) in wild type (W303-1A) cells treated with 2 µM oligomycin. The blots were also probed with an antibody that recognizes porin as a protein loading control.

27708226 24450340 20444419 19735676

Protocol

Cell Research:[2]
+ Expand
  • Cell lines: H1229
  • Concentrations: 100 ng/ml
  • Incubation Time: 1 h
  • Method: ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 10 mg/mL (12.64 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
15 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 791.06
Formula

C45H74O11

CAS No. 579-13-5
Storage powder
in solvent
Synonyms MCH 32

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID