Oligomycin A

For research use only.

Catalog No.S1478 Synonyms: MCH 32

32 publications

Oligomycin A Chemical Structure

CAS No. 579-13-5

Oligomycin A (MCH 32) is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 257 In stock
USD 135 In stock

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Selleck's Oligomycin A has been cited by 32 publications

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Biological Activity

Description Oligomycin A (MCH 32) is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.
Targets
ATP synthase [1]
(Cell-free assay)
In vitro

Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.Oligomycin A inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. The inhibition of ATP synthesis by oligomycin A will significantly reduce electron flow through the electron transport chain; however, electron flow is not stopped completely due to a process known as proton leak or mitochondrial uncoupling. [1] In a group of cancer cells, Oligomycin at 100 ng/ml completely inhibits oxidative phosphorylation activity in 1 h and induces various levels of glycolysis gains by 6 h. [2] Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis. [3] Oligomycin inhibits mitochondrial respiration in melanoma, sensitizes melanoma cells to therapy, blocks the emergence of the slow-cycling, long-term tumor-maintaining melanoma cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2 cells M{LVNmZ2dmO2aX;uJIF{e2G7 NVftR3FoOjVuIEWwMEA4PSxiMUCwJO69VQ>? Mki2[YxmfmG2ZXSgeIhmKGeudXPvd4UhfXC2YXvlJIxmfmWuIHL1eEBvd3RiZH;z[U1l\XCnbnTlcpQ> NVWz[ZJ2OzB{MEW1O|I>
Vero cells NHfxd3NHfW6ldHnvckBie3OjeR?= NGi5fIExNjBzLDCwMlEtKDFuIHHu[EAyOCEQvF2= NF3SWWViKGSnY4LlZZNmKGmwIITo[UB3cWGkaXzpeJkhd2ZiVnXyc{Bk\WyuczCoglIxLSC{ZXT1Z5Rqd25rIHH0JIlv[3KnYYPpcoch[2:wY3XueJJifGmxboOu NYDUdWxkOjZ6NUm3OFU>
ISE6 cells M2jyO2Z2dmO2aX;uJIF{e2G7 MX6wMlAyNCByLkGsJFEtKGGwZDCxNEDPxE1? MWHhJIRm[3KnYYPlJIlvKHSqZTD2bYFjcWyrdImgc4YhUVOHNjDj[YxteyBqfk[wKUkh[XRiaX7jdoVie2mwZzDjc45k\W62cnH0bY9vey5? MmHVNlY5PTl5NEW=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-GCN2 / GCN2 / p-eIF2α / eIF2α / ATF4 / xCT; 

PubMed: 27708226     


Western blot analysis of the GCN2-eIF2α-ATF4-xCT pathway in the SC-M1 cells under oligomycin (O, 1 μg/ml) and N-acetyl cysteine (NAC, N, 5 mM) treatments for 24 h. The immunoblot values were normalized to α-tubulin.

pAkt / Her2 / Raf / Hsp70 / Hsp90 ; 

PubMed: 24450340     


Western blot for Hsp90 client proteins (pAkt, Her2, and Raf) and Hsp70, Hsp90, and F1F0 ATP synthase using MCF7 cell lysates after 24 or 48 h of incubation with the indicated amount of vehicle (0.25% DMSO) or oligomycin A. The calculated EC50 value of oligomycin A against the MCF7 cell line was 7.35 μM.

p-T389 S6K1 / S6K1 / p-T172 AMPK / AMPK ; 

PubMed: 20444419     


TSC2+/+MEFs were treated with either 10μM oligomycin at the indicated times. * denotes the addition of a fresh aliquot of either ATP-depleting agent following a 60min incubation with the initial inhibitor. The data shown are representative of at least three independent experiments.

Cox1p / Cox2p / Cox3p / Cox4p ; 

PubMed: 19735676     


Western-blot analyses of the steady state concentrations of several COX and ATPase subunits (indicated in the margin) in wild type (W303-1A) cells treated with 2 µM oligomycin. The blots were also probed with an antibody that recognizes porin as a protein loading control.

27708226 24450340 20444419 19735676

Protocol

Cell Research:[2]
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  • Cell lines: H1229
  • Concentrations: 100 ng/ml
  • Incubation Time: 1 h
  • Method: ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 10 mg/mL (12.64 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
15 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 791.06
Formula

C45H74O11

CAS No. 579-13-5
Storage powder
in solvent
Synonyms MCH 32
Smiles CCC1CCC2C(C(C(C3(O2)CCC(C(O3)CC(C)O)C)C)OC(=O)C=CC(C(C(C(=O)C(C(C(C(=O)C(C(C(CC=CC=C1)C)O)(C)O)C)O)C)C)O)C)C

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID