Puromycin 2HCl

Catalog No.S7417 Batch:S741710

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Technical Data

Formula

C22H29N7O5.2HCl
Molecular Weight 544.43 CAS No. 58-58-2
Solubility (25°C)* In vitro DMSO 100 mg/mL (183.67 mM)
Water 100 mg/mL (183.67 mM)
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
saline
100.000mg/ml Taking the 1 mL working solution as an example, add 100 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.
Homogeneous suspension
CMC-NA
≥5mg/ml Homogeneous suspension for Oral and Intraperitoneal Injection. Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Puromycin 2HCl is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinated expression of genes during cell differentiation.
In vitro

The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. [1]

Puromycin can be used for the selection of recombinant cells from noncultured cells. [2]
In vivo

Puromycin (aminonucleoside) inhibits protein synthesis leading to proteinuria & glomerulosclerosis/nephrosis.[4]

Protocol (from reference)

Cell Assay:

[3]
  • Cell lines

    HepG2 cells 

  • Concentrations

    1.6 μM

  • Incubation Time

    72 h

  • Method

    Cells were treated with different concentrations of drug for 72 h.
Animal Study:

[4]
  • Animal Models

    Male Sprague-Dawley rats

  • Dosages

    5 mg/100 g

  • Administration

    i.v.

Selleck's Puromycin 2HCl has been cited by 218 publications

RNA-binding proteins DND1 and NANOS3 cooperatively suppress the entry of germ cell lineage [ Nat Commun, 2025, 16(1):4792] PubMed: 40410171
SAMD9 senses cytosolic double-stranded nucleic acids in epithelial and mesenchymal cells to induce antiviral immunity [ Nat Commun, 2025, 16(1):3756] PubMed: 40263291
Homeodomain protein PRRX1 anchors the Ku heterodimers at DNA double-strand breaks to promote nonhomologous end-joining [ Nucleic Acids Res, 2025, 53(6)gkaf200] PubMed: 40114375
USP9X integrates TGF-β and hypoxia signalings to promote ovarian cancer chemoresistance via HIF-2α-maintained stemness [ Cell Death Dis, 2025, 16(1):312] PubMed: 40246814
NF-κB-mediated EAAT3 upregulation in antioxidant defense and ferroptosis sensitivity in lung cancer [ Cell Death Dis, 2025, 16(1):124] PubMed: 39987248
The m5C methyltransferase NSUN2 promotes progression of acute myeloid leukemia by regulating serine metabolism [ Cell Rep, 2025, 44(5):115661] PubMed: 40343793
The low-dose CHK1 inhibitor prexasertib triggers VDAC1 dephosphorylation to activate mtDNA-STING signaling and synergize immunotherapy [ Cell Rep, 2025, 44(5):115605] PubMed: 40249707
FOSL1 transcriptionally dictates the Warburg effect and enhances chemoresistance in triple-negative breast cancer [ J Transl Med, 2025, 23(1):1] PubMed: 39748430
PRRSV-2 nsp2 Ignites NLRP3 inflammasome through IKKβ-dependent dispersed trans-Golgi network translocation [ PLoS Pathog, 2025, 21(1):e1012915] PubMed: 39869629
PDLIM3 Regulates Migration and Invasion of Head and Neck Squamous Cell Carcinoma via YAP-Mediated Epithelial-Mesenchymal Transition [ Int J Mol Sci, 2025, 26(7)3147] PubMed: 40243891

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.