Blasticidin S HCl

Catalog No.S7419

For research use only.

Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes, and acts as a DNA and protein synthesis inhibitor, used to select transfected cells carrying bsr or BSD resistance genes.

Blasticidin S HCl Chemical Structure

CAS No. 3513-03-9

Selleck's Blasticidin S HCl has been cited by 12 Publications

Purity & Quality Control

Choose Selective Selection Antibiotics for Transfected Cell Inhibitors

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Biological Activity

Description Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes, and acts as a DNA and protein synthesis inhibitor, used to select transfected cells carrying bsr or BSD resistance genes.
Targets
protein synthesis [1] DNA synthesis [1]
In vitro

Blasticidin S inhibits protein synthesis by acting on the step of peptide transfer from peptidyl-sRNA to incoming aminoacyl-sRNA. [1] Blasticidin S also significantly inhibits DNA synthesis independent of their effect on protein synthesis. [2] Blasticidin S kills the cells more rapidly than G418, which makes blasticidin S-resistance gene (bsr) a selectable marker for mammalian cells. [3] Besides, Blasticidin S also efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. [4]

Assay
Methods Test Index PMID
Western blot p-IκBα / T-IκBα / β-actin 16184196
Growth inhibition assay Cell viability 24782309
mRNA blot MGG_00447 / MGG_03736 / MGG_04855 / MGG_09931 / 18s rRNA ; MGG_00447 / MGG_03736 / MGG_04855 / MGG_09931 / Beta tubulin 26889110

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 458.9
Formula

C17H27ClN8O5

CAS No. 3513-03-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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