Blasticidin S HCl

For research use only.

Catalog No.S7419

10 publications

Blasticidin S HCl Chemical Structure

CAS No. 3513-03-9

Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes, and acts as a DNA and protein synthesis inhibitor, used to select transfected cells carrying bsr or BSD resistance genes.

Selleck's Blasticidin S HCl has been cited by 10 publications

Purity & Quality Control

Choose Selective Selection Antibiotics for Transfected Cell Inhibitors

Biological Activity

Description Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes, and acts as a DNA and protein synthesis inhibitor, used to select transfected cells carrying bsr or BSD resistance genes.
Targets
protein synthesis [1] DNA synthesis [1]
In vitro

Blasticidin S inhibits protein synthesis by acting on the step of peptide transfer from peptidyl-sRNA to incoming aminoacyl-sRNA. [1] Blasticidin S also significantly inhibits DNA synthesis independent of their effect on protein synthesis. [2] Blasticidin S kills the cells more rapidly than G418, which makes blasticidin S-resistance gene (bsr) a selectable marker for mammalian cells. [3] Besides, Blasticidin S also efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. [4]

Assay
Methods Test Index PMID
Western blot
p-IκBα / T-IκBα / β-actin; 

PubMed: 16184196     


(D) WT BMMs were retrovirally transduced with empty vector (PMX) or vector expressing FHL2 and selected with blasticidin S, hydrochloride for 3 days. Selected cells were starved for 3 hours and stimulated with RANKL (100 ng/ml) for up to 30 minutes. Phosphorylated (p) and total (T) IκBα were determined by immunoblot. β-actin served as loading control.

16184196
Growth inhibition assay
Cell viability; 

PubMed: 24782309     


D, WT KBM7 cells, WT NF-κB reporter cells, LRRC8DGT1 cells, and LRRC8DGT2 cells treated with varying concentrations of BlaS. Cell viability was determined after 24 h of treatment using the CellTiter Glo assay, and results are plotted as percentage viability of treated cells compared with untreated cells.

24782309
mRNA blot
MGG_00447 / MGG_03736 / MGG_04855 / MGG_09931 / Beta tubulin; 

PubMed: 26889110     


RT-PCR of mRNA expression levels of ABC transporter genes. Commercial fungicides EDDP, IBP, and blasticidin S were used to treat the mycelia of M. oryzae on PDA for 3 h. Lane 1: No treatment, Lane 2: blasticidin S, Lane 3: EDDP (Edifenphos), Lane 4: IBP (Iprobenfos). Each mRNA expression level was normalized to the expression level of beta tubulin, a house-keeping gene.

MGG_00447 / MGG_03736 / MGG_04855 / MGG_09931 / 18s rRNA; 

PubMed: 26889110     


RT-PCR of ABC transporter gene mRNA expression levels after combinational treatment with blasticidin S and tetrandrine or with L. foenum-graecum Herba extract. Total RNA was isolated in M. oryzae mycelia treated by single or simultaneous treatment of blasticidin S with tetrandrine or L. foenum-graecum Herba extract for 3 h. (A) Inhibition of ABC transporter gene expression by tertrandrine. + : Treatment, − : None treatment. (B) Inhibition of ABC transporter gene expression by the extract of L. foenum-graecum Herba. + : Treatment, − : None treatment.

26889110

Protocol

Solubility (25°C)

In vitro Water 91 mg/mL (198.3 mM)
DMSO Insoluble
Ethanol Insoluble

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Chemical Information

Molecular Weight 458.9
Formula

C17H27ClN8O5

CAS No. 3513-03-9
Storage powder
in solvent
Synonyms N/A
Smiles CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID