Geldanamycin (NSC 122750)

For research use only.

Catalog No.S2713

25 publications

Geldanamycin (NSC 122750) Chemical Structure

CAS No. 30562-34-6

Geldanamycin (NSC 122750) is a natural existing HSP90 inhibitor with Kd of 1.2 μM, specifically disrupts glucocorticoid receptor (GR)/HSP association. Geldanamycin attenuates virus infection-induced ALI (acute lung injury)/ARDS (acute respiratory distress syndrome) by reducing the host's inflammatory responses.

Selleck's Geldanamycin (NSC 122750) has been cited by 25 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Geldanamycin (NSC 122750) is a natural existing HSP90 inhibitor with Kd of 1.2 μM, specifically disrupts glucocorticoid receptor (GR)/HSP association. Geldanamycin attenuates virus infection-induced ALI (acute lung injury)/ARDS (acute respiratory distress syndrome) by reducing the host's inflammatory responses.
Targets
p185 [4]
(SKBr3 cells)
HSP90 (N-terminal domain) [1]
(Cell-free assay)
HSP90 [1]
(Cell-free assay)
70 nM 0.78 μM(Kd) 1.2 μM(Kd)
In vitro

Geldanamycin binds in the ATP-binding site in the N-terminus domain of Hsp90s (residues 1-220). Geldanamycin inhibits the ATPase activity of Hsp90 in a dose-dependent manner. [1] Geldanamycin causes a dose-dependent G2 arrest and reversible inhibiton o f entry into the S phase in A2780 human ovarian cell line. This inhibition is accompanied by p53 increase and finally demonstrated to be p53 dependent. [2] Geldanamycin causes polyubiquitination and proteasomal degradation of the p185 receptor protein-tyrosin kinase and shows a IC50 with 70 nM. [3, 4] Geldanamycin is a typical anti-tumor reagent, shows a mean GI50 with 0.18 μM against the panel of 60 human tumor cell lines. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A2780 cells NXry[WVvWHKxbHnm[ZJifGmxbjDhd5NigQ>? M{Ho[GNwdXCxdX7kJJdieyCndnHseYF1\WRiZn;yJIFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5ib4\hdolidiClYYLjbY5wdWFiY3XscEBtcW6nIFGyO|gxNCCLQ{WwQVMvPCEQvF2= MkjQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF3MUSxOFUoRjFzNUG0NVQ2RC:jPh?=
SW620 cell M{e2NGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoH2TY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3RiaIXtZY4h[2:ub4LlZ5RidCClYYLjbY5wdWFiU2e2NlAh[2WubDDsbY5meyxiSVO1NF03NjJibl2= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTZ3OEi3PUc,OTV4NUi4O|k9N2F-
MCF-7 cell M4HwVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoT3TY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3RiaIXtZY4h[nKnYYP0JINidmOncjDNR2YuPyClZXzsJIxqdmW|LDDJR|UxRTZwNTDuUS=> M3LmTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NkW4PFc6Lz5zNU[1PFg4QTxxYU6=
K562 cell NEfjeYRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{\5W2lvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IHj1cYFvKGyndXvlcYliKEt3NkKgZ4VtdCCuaX7ld{whUUN3ME2yNk4yKG6P M4D1flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NkW4PFc6Lz5zNU[1PFg4QTxxYU6=
HT-29 cell MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3r0XmlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IHj1cYFvKGOxbH;y[YN1[WxiY3HyZ4lvd22jIFjUMVI6KGOnbHygcIlv\XNuIFnDOVA:OjRwNTDuUS=> Ml\3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV4NUi4O|koRjF3NkW4PFc6RC:jPh?=
SK-BR-3 NWPCO25FTnWwY4Tpc44h[XO|YYm= M1W4WlUxKG2pL3vn M2O2SmlvcGmkaYTpc44hd2Zib37jc4dmdmVicILv[JVkfCCyMUi1JIVz[kJvMjDmdo9uKGi3bXHuJIJz\WG|dDDjZY5k\XJiY3XscJMpW0tvQmKtN{Bk\WyuczmgZZQhPTBibXevb4ch\G:|ZTygTWM2OD1yLkC3{txO M3nrSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzd3NkK5NVEoRjd3NkK5NVE9N2F-
SK-BR-3 NFjXVnBHfW6ldHnvckBie3OjeR?= NWG1XWtqUW5idnn0do8hcW6qaXLpeIlwdiCxZjDwNVg2KGW{YlKtNkBwdmOxcILveIVqdiCrbjDoeY1idiCkcnXhd5Qh[2GwY3XyJHNMNUKULUOgZ4VtdHNuIFnDOVA:OC5yN988US=> M{[xelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzd3NkK5NVIoRjd3NkK5NVI9N2F-
MCF-7 M{PVSWZ2dmO2aX;uJIF{e2G7 M4O1VWlvcGmkaYTpc44hd2Zic4TlZYR6NXO2YYTlJIV5eHKnc4Ppc44hd2ZiSFXSNkBqdiCPQ1[tO{BjemWjc4SgZ4Fv[2W{IHPlcIx{NCCLQ{WwQVAvODYQvF2= NGLUboQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEO0NFYxPSd-MUCzOFA3ODV:L3G+
MCF-7 MmTuSpVv[3Srb36gZZN{[Xl? NImzNYNKdmirYnn0bY9vKG:oIIP0[YFlgS2|dHH0[UBmgHC{ZYPzbY9vKG:oIFXSJIlvKE2FRj23JIJz\WG|dDDjZY5k\XJiY3XscJMtKEmFNUC9NE4xPs7:TR?= NH;hOWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEO0NFYxPSd-MUCzOFA3ODV:L3G+
MCF-7 M{nQNGZ2dmO2aX;uJIF{e2G7 NV7BS5V4UW6qaXLpeIlwdiCxZjDzeIVi\Hlvc4TheIUh\XiycnXzd4lwdiCxZjDSZYYuOSCrbjDNR2YuPyCkcnXhd5Qh[2GwY3XyJINmdGy|LDDJR|UxRTBwMt88US=> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODN2ME[wOUc,OTB|NEC2NFU9N2F-
SKBR-3 NG\BVY9HfW6ldHnvckBie3OjeR?= NGXJcYhG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDmc5IhUGW{LUKg[IVoemGmYYTpc44hcW5iU1vCVk0{KGOnbHzzMEBEd26lZX70doF1cW:wPUZOwG0> NWf0S2Y6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3NVM1OTBpPkG1O|E{PDFyPD;hQi=>
HEK293 NGjURZdHfW6ldHnvckBie3OjeR?= MkDVTY5pcWKrdHnvckBw\iCKU2C5NEBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W|c3XkJIF{KGWoZnXjeEBwdiCDa4SxPpAzPyCrboTldoFkfGmxbjDjc41xdGW6ZYOgZpkhTVmIUDDhcoQwd3JiWV\QJHZmdnW|IH\yZYdu\W62IHLhd4VlKHKncH;yeIVzKGenbnWgZZN{[Xl? Mkj6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
LS174T M{\1PGN6fG:2b4jpZ4l1gSCjc4PhfS=> NVzZV3Q5S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVFNzN{TUJINmdGy|IHL5JG1VWyCjc4PhfUwhUUN3ME2wMlQ2|ryP NIi5Xok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{CzOFE{PSd-MUewN|QyOzV:L3G+
rat neuronal cells NF\mbXJEgXSxdH;4bYNqfHliYYPzZZk> NX\VfZh7OTBidV2= MWmyOEBpenN? NEThfIREgXSxdH;4bYNqfHliYXfhbY5{fCC{YYSgcoV2em:wYXygZ4VtdHNiYYSgNVAhfU1iYX\0[ZIhOjRiaILz M4H0XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5Mke2Olc6Lz5zN{K3OlY4QTxxYU6=
P19 NGLje2pEgXSxdH;4bYNqfHliYYPzZZk> MX[xPEBpenN? MV7DfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDQNVkh[2WubIOgZYZ1\XJiMUigbJJ{NCCLQ{WwQVAvOc7:TR?= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR2MkW2OUc,OTd2NEK1OlU9N2F-
MCF7 MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVLHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IHThfZMh[nliU2LCJIF{e2G7LDDHTVUxRTN3LkdOwG0> NXzhS2FSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4OlkxQThpPkG3PFY6ODl6PD;hQi=>
HCT116 MoLwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWLHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIR3QyOTZiY3XscJMtKEeLNUC9NE4xOjIQvF2= M1HN[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkSzO|A{Lz5zOEK0N|cxOzxxYU6=
DLD1 MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXHlSYZvT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hTEyGMTDj[YxteyxiR1m1NF0xNjB|N988US=> NE\a[m89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0N|cxOyd-MUiyOFM4ODN:L3G+
KPL4 M3fTXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIOwU|ZIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBMWEx2IHPlcIx{NCCJSUWwQVAvODV7zszN NVTa[FZHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFM4ODNpPkG4NlQ{PzB|PD;hQi=>
A549 NIjCSY5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mne1S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gRVU1QSClZXzsd{whT0l3ME2wMlA3PM7:TR?= NW\kb5lKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFM4ODNpPkG4NlQ{PzB|PD;hQi=>
KPL4 NXvHWJNuTnWwY4Tpc44h[XO|YYm= NEHUZ5Q1OCCqcoO= MYDJcohq[mm2aX;uJI9nKEi|cEmwJIlvKGi3bXHuJGtRVDRiY3XscJMh[XO|ZYPz[YQh[XNiZHXwcIV1cW:wIH;mJGVz[kJ{IHzleoVtKGGodHXyJFQxKGi{czygUWVERTBwMUJOwG0> NWDQRWNRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFM4ODNpPkG4NlQ{PzB|PD;hQi=>
KPL4 M3LaZWZ2dmO2aX;uJIF{e2G7 NETzbmc1OCCqcoO= NYLKNJlkUW6qaXLpeIlwdiCxZjDId5A6OCCrbjDoeY1idiCNUFy0JINmdGy|IHHzd4V{e2WmIHHzJIRmeGyndHnvckBw\iCUYX[tNUBt\X[nbDDh[pRmeiB2MDDodpMtKE2HQ{2wMlM{|ryP MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2M{ewN{c,OTh{NEO3NFM9N2F-
Jurkat NVewOWxuTnWwY4Tpc44h[XO|YYm= NEDrPFcyKHWP M2f2dGlvcGmkaYTpc44hd2ZidH:gTHNRQTBiaX6gSmFFTCCmZX\pZ4lmdnRiaIXtZY4hUnW{a3H0JINmdGy|IHHzd4V{e2WmIHHzJHJKWCCycn;0[YlvKGSnZ4Lh[IF1cW:wIHH0JFEhfU1iYomgW4V{fGW{bjDicI91 NUTKUFBrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0NFg4OTNpPkG4OFA5PzF|PD;hQi=>
HEK293T NUm3PY5yTnWwY4Tpc44h[XO|YYm= NVK5O|N5UW6qaXLpeIlwdiCxZjDUUmYu[WyyaHGtbY5lfWOnZDDOSk1s[XCyYVKgZYN1cX[jdHnvckBmgHC{ZYPz[YQhcW5iSFXLNlk{XCClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYm= M3TQXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEC4O|E{Lz5zOESwPFcyOzxxYU6=
Jurkat MmniSpVv[3Srb36gZZN{[Xl? M1nHb2lvcGmkaYTpc44hd2ZiVF7GMYFteGijLXnu[JVk\WRiTl[tb4FxeGGEIHHjeIl3[XSrb36g[ZhxemW|c3XkJIlvKE[DRFSg[IVncWOrZX70JIh2dWGwIFr1dotifCClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYm= NYHDRmNnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0NFg4OTNpPkG4OFA5PzF|PD;hQi=>
SKBR3 MX7GeY5kfGmxbjDhd5NigQ>? NXzMems5OjRiaILz M2\hfWlvcGmkaYTpc44hd2ZiSIPwPVAudWWmaXH0[YQhUEWUMjDk[Ydz[WSjdHnvckBqdiCqdX3hckBUU0KUMzDj[YxteyCjZoTldkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2 MkTwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh6MU[xNVEoRjF6OEG2NVEyRC:jPh?=
SKBR3 MmC3SpVv[3Srb36gZZN{[Xl? Mnv5NlQhcHK| NYX3bXk4UW6qaXLpeIlwdiCxZjDId5A6OC2vZXTpZZRm\CCUYX[g[IVoemGmYYTpc44hcW5iaIXtZY4hW0uEUkOgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KFenc4Tldo4h[myxdB?= NEPuSFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEixOlEyOSd-MUi4NVYyOTF:L3G+
HuH7 MWjBcpRqfmm{YXygZZN{[Xl? M{PaWFMh\GG7cx?= M3PCOGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhmeGG2aYTpd{BEKH[rcoXzJIdmdm:2eYDlJFFjKEOxbkGgbY5n\WO2ZXSgbY4hcHWvYX6gTJVJPyClZXzsd{Bie3Onc4Pl[EBieyCJQWDETEBTVkFib4KgNVhUKHKUTlGgcIV3\WxiYX\0[ZIhOyCmYYnzJIJ6KHGUVD3QR3Ih[W6jbInzbZMtKEWFNUC9NE4xODB{Nd88US=> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDl|NkG5NUc,OTh7M{[xPVE9N2F-
HuH7 NVTrcpFSSW62aY\pdoFtKGG|c3H5 NVOzOXJVOyCmYYnz M2XaPGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhmeGG2aYTpd{BEKH[rcoXzJIdmdm:2eYDlJFFjKEOxbkGgbY5n\WO2ZXSgbY4hcHWvYX6gTJVJPyClZXzsd{Bie3Onc4Pl[EBieyCJQWDETEBTVkFib4KgNVhUKHKUTlGgcIV3\WxiYX\0[ZIhOyCmYYnzJJNmdGWldHXkJJdqfGhiNECgcm0hUEOYLUe5OkBidmRiOECwJI5OKGKxY3XwdoV3cXJiYomgdXJVNVCFUjDhcoFtgXOrczygSWM2OD1yLkCwNVLPxE1? Mo\sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh7M{[xPVEoRjF6OUO2NVkyRC:jPh?=
SH-SY5Y NGi4b|dP\XW{b4Dyc5Rm[3Srb36gZZN{[Xl? NYDsXoxJVmW3cn;wdo91\WO2aX;uJIFo[Wmwc4SgZoV1[S2jbYnsc4llKHCncITp[IUhOS12Mj3pcoR2[2WmIITvfIlkcXS7IHnuJIh2dWGwIGPIMXN[PVliY3XscJMh[XO|ZYPz[YQh[XNibHHjeIF1\SCmZXj5[JJw\2WwYYPlJJJmdGWjc3WsJGVEPTB;MD6wOFI3Qc7:TR?= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTF|OEi1PUc,OTlzM{i4OVk9N2F-
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SKBR3 MYTDfZRwfG:6aXPpeJkh[XO|YYm= NV;YWIR5PzJiaILz NGnNXWpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUU0KUMzDj[YxteyCjZoTldkA4OiCqcoOgZpkh[2WubITpeIVzNWeubzDhd5NigSxiSVO1NF0xNjB2Md88US=> NX3De|ltRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0NFU2OjhpPkG5OFA2PTJ6PD;hQi=>
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... Click to View More Cell Line Experimental Data

In vivo Geldanamycin (50 mg//kg) shows 30% inhibition on pl85-associated phosphotyrosine levels in FRE/erbB-2 mice. [6]

Protocol

Kinase Assay:

[1]

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Isothermal Titration Calorimetry (ITC) of Nucelotide Binding:

The titration experiments are performed using the MSC system. In each experiment, 16 aliquots of 15 μL of geldanamycin (300 μM in 1% DMSO) are injected into 1.3 mL of protein (31 μM in 20 mMTris-HCl, pH 7.5, 1 mMEDTA) at 25 °C, and the resulting data are fit after subtracting the heats of dilution. Heats of dilution are determined in separate experiments from addition of geldanamycin into buffer and buffer into protein. No evidence for binding of DMSO in the nucleotide binding site is observed. Titration data are fit using a nonlinear least-squares curve-fitting algorithm with three floating variables: stoichiometry, binding constant (Kb) 1/Kd), and change of enthalpy of interaction (ΔH°). Dissociation constants estimated for geldanamycin binding to intact yeast Hsp90 is 1.22 μM, and for binding to Hsp90 N-terminal domain is 0.78 μM. No meaningful heat is observed with binding to the C-terminal fragment.
Cell Research:

[2]

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  • Cell lines: A2780 human ovarian cell line
  • Concentrations: 0.001-10 μM
  • Incubation Time: 3 hours
  • Method:

    Exponentially growing cells are treated with Geldanamycin and at various times DNA synthesis is assessed by incorporation of bromodeoxyuridine (BrdUrd) and flow cytometric analysis. No marked difference in total cell number is noted during this time course for treated and untreated cultures. BrdUrd (10 μM) is incorporated over a 4-h incubation period at 37 °C and cells are harvested and fixed in 70% ethanol. After denaturation of the DNA with 2 N HC1, cells are incubated with an anti-BrdUrd mouse monoclonal antibody followed by a fluorescein isothiocyanate (FITC)-linked goat anti-mouse IgG. Cells are stained for 30 minutes at room temperature with propidium iodide and analysed by flow cytometry using a Coulter EPICS Profile Analyzer.


    (Only for Reference)
Animal Research:

[6]

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  • Animal Models: FRE/erbB-2 tumors in nu/nu mice
  • Dosages: 50 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 36 mg/mL warmed (64.21 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 560.64
Formula

C29H40N2O9

CAS No. 30562-34-6
Storage powder
in solvent
Synonyms N/A
Smiles CC1CC(C(C(C=C(C(C(C=CC=C(C(=O)NC2=CC(=O)C(=C(C1)C2=O)OC)C)OC)OC(=O)N)C)C)O)OC

In vivo Formulation Calculator (Clear solution)

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Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Molecular Weight Calculator

Molecular Weight Calculator

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT00003969 Completed Drug: tanespimycin Unspecified Adult Solid Tumor Protocol Specific Cancer Research UK|National Cancer Institute (NCI) August 1998 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Antineoplastic and Immunosuppressive Antibiotics Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID