Honokiol (NSC 293100)

Catalog No.S2310

For research use only.

Honokiol (NSC 293100) is the active principle of magnolia extract that inhibits Akt-phosphorylation and promotes ERK1/2 phosphorylation. Honokiol causes G0/G1 phase arrest, induces apoptosis, and autophagy via the ROS/ERK1/2 signaling pathway. Honokiol inhibits hepatitis C virus (HCV) infection. Phase 3.

Honokiol (NSC 293100) Chemical Structure

CAS No. 35354-74-6

Selleck's Honokiol (NSC 293100) has been cited by 26 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Honokiol (NSC 293100) is the active principle of magnolia extract that inhibits Akt-phosphorylation and promotes ERK1/2 phosphorylation. Honokiol causes G0/G1 phase arrest, induces apoptosis, and autophagy via the ROS/ERK1/2 signaling pathway. Honokiol inhibits hepatitis C virus (HCV) infection. Phase 3.
Targets
Akt-phosphorylation [1] MEK [8]
()
In vitro

Honokiol shows pro-apoptotic effects in melanoma, sarcoma, myeloma, leukemia, bladder, lung, prostate, oral squamous cell carcinoma and colon cancer cell lines. Honokiol is effective on inducing apoptosis in SVR angiosarcoma cells. Treatment of SVR cells with honokiol causes decreased phosphorylation of MAP kinase, akt, and c-src. In addition, honokiol potentiates TRAIL-mediated apoptosis, and honokiol cytotoxicity is partially abrogated by neutralizing antibodies to TRAIL. Honokiol also has direct antiangiogenic activity, in that honokiol blocks the phosphorylation and rac activation due to VEGF-VEGFR2 interactions. [1] Honokiol causes apoptosis in CLL cells through activation of caspase 8, followed by caspase 9 and 3 activation. Honokiol prevents interleukin-4-mediated survival of CLL cells, and potentiats the cytotoxicity of chlorambucil, fludarabine, and cladribine. [3] Honokiol kills myeloma cells from relapsed patients at doses that does not kill PBMCs. Caspase 3, 7, 8, and 9 are induced by honokiol treatment, as well as PARP cleavage. [2] Honokiol is found to induce apoptosis in the colon cancer cell lines RKO. [4] Honokiol potentiates apoptosis, suppresses osteoclastogenesis, and inhibits invasion through modulation of nuclear factor-kappaB activation pathway. [5] Honokiol may act as a potent anti-inflammatory agent with multipotential activities due to an inhibitory effect on the PI3K/Akt pathway. [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human UACC-903 cells NVPhcG9KS3m2b4TvfIlkcXS7IHHzd4F6 NIHnZ4hEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWSUOFLUmwN{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTTJIF{e2G7LDDJR|UxRTVwMTFOwG0> NELT[YczOjV|M{m4Ny=>
Vero E6 cells MYPGeY5kfGmxbjDhd5NigQ>? M{jU[mFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHNCWlNiY3;yc45ifmm{dYOgbY4hXmW{bzDFOkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wIHL5JGVNUVODLDDFR|UxRTZwNTFOwG0> NFzSPYYyPzZ4M{WzPS=>
human UACC-903 cells M{H3V2N6fG:2b4jpZ4l1gSCjc4PhfS=> MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDVRWNENTlyMzDj[YxteyCjZoTldkAzPCCqcoOgZpkhVVSVIHHzd4F6NCCLQ{WwQVcvPDVizszN NUH6[I9bOjJ3M{O5PFM>
human A549 cells M3:xe2N6fG:2b4jpZ4l1gSCjc4PhfS=> NXflNHQxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSVIHHzd4F6NCCLQ{WwQVcvPzVizszN NYm1PXFkOjJ3M{O5PFM>
human CEM cells M4LUU2N6fG:2b4jpZ4l1gSCjc4PhfS=> NG\mVJBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBETU1iY3XscJMtKEmFNUC9NVAvQSEQvF2= MkXsNVc2QDd3N{K=
HEK293 cells NVzrcm1wTnWwY4Tpc44h[XO|YYm= NIrM[YlC\2:waYP0JIFkfGm4aYT5JIF1KFK[UnHsdIhiKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCjZoTldkA1QCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTFzLkig{txO MWOyNFY6PTR5Mh?=
human A549 cell NHqweoNEgXSxdH;4bYNqfHliYYPzZZk> NUDHc5JCOjRiaB?= NHnlVm5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFI1KGi{czDifUBOXFNiYYPzZZktKEmFNUC9NVIvPTFizszN MYiyNlU{Ozl6Mx?=
human HT-29 cells MUXDfZRwfG:6aXPpeJkh[XO|YYm= M2rOOlczKGh? NWHpeWJWS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUFRvMkmgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF0yOy5{NDFOwG0> NIjhZ3YzOjV|M{m4Ny=>
human PBM cells M{jyfmN6fG:2b4jpZ4l1gSCjc4PhfS=> MnvhR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVGJOKGOnbHzzMEBKSzVyPUG2MlEh|ryP M1rMTFE4PTh5NUey
Hep-G2 cells NV3LUZNSS3m2b4TvfIlkcXS7IHHzd4F6 NXrmbYg{S3m2b4TvfIlkcXS7IH;mJINwdXCxdX7kJIFo[Wmwc4SgbJVu[W5ibHn2[ZIhfHWvb4KgZ4VtdCCuaX7lJEhJ\XBvR{KpJJdieyCmZYTldo1qdmWmLDDJR|UxRTF4LkWg{txO Mo\HNVU2QDJ2M{K=
human HepG2 cells MUTQdo9tcW[ncnH0bY9vKGG|c3H5 M4PUeFI1KGh? NFPRSJFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjldGczKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MU[uOUDPxE1? MmDPNlE5PTN7OUG=
human K562 cells M4rPW3Bzd2yrZnXyZZRqd25iYYPzZZk> MVzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEt3NkKgZ4VtdHNiYomgUXRVKGG|c3H5MEBKSzVyPUKxMlEh|ryP MXmxPVU5QTZ5OB?=
Vero cells MlXhR5l1d3SxeHnjbZR6KGG|c3H5 M4TRfGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KF[ncn:gZ4VtdHNuIFnDOVA:OjJwNTFOwG0> NHjVNogyPjd{Mk[2OC=>
human A2780 cells NWPUWVRnWHKxbHnm[ZJifGmxbjDhd5NigQ>? MnrQRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDjbZNxdGG2aX6td4Vve2m2aY\lJIh2dWGwIFGyO|gxKGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NF0{OC53IN88US=> NWHYZlI2OTl3OEm2O|g>
human SPC-A1 cells MUjQdo9tcW[ncnH0bY9vKGG|c3H5 NH\N[HZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPQR{1COSClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVA:OzZwMTFOwG0> M36yNlE6PTh7Nke4
HUVEC cells MkLYSpVv[3Srb36gZZN{[Xl? NWS4R41uOjBizszN NXT6U45rOjRiaB?= M1\oXGFvfGmvaXfyZZRwenliYXP0bZZqfHliYXfhbY5{fCCqdX3hckBJXV[HQzDj[YxteyCjdDCyNEB2VSCjZoTldkAzPCCqcoOgZpkhf2:3bnStbIVidGmwZzDhd5NigQ>? MoDSNlE5PTN7OUG=
HEK293 cells M1rEOmZ2dmO2aX;uJIF{e2G7 MX:yOE01QCCq NULUPIExSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBTYFJvYXzwbIEh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bkd2W6cILld5Nqdmdid3n0bEBxS02[LXLleIEu\2GuIHHmeIVzKDJ2IITvJFQ5KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[Xl? NFLmfnozPDl3OUm4Oy=>
human SH-SY5Y cells MmXmSpVv[3Srb36gZZN{[Xl? NGrlTGIyOCEQvF2= MnzSN|AhdWmwcx?= MYXO[ZVzd3C{b4TlZ5RqfmViYXP0bZZqfHliaX6gbJVu[W5iU1itV3k2YSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGNJWCCjbnSgWGJJWC2rbnT1Z4VlKGOnbHyg[IVifGhiYYSgNVAhfU1iaX7jeYJifGWmIH\vdkA{OCCvaX7zJJBzcW:{IITvJINp[WyuZX7n[UBu\WG|dYLl[EBi\nSncjCzJIhzeyCkeTDNWHQh[XO|YYm= MVuyNlE1OjV|OR?=
Assay
Methods Test Index PMID
Western blot p-EGFR / p-AKT / p-STAT3 / p-ERK / p-GSK3α/β / Bax / p-pRb / p-BAD / IκBα / CDK2 / CDK4 / Cyclin D1 ; p-Lyn / Lyn / p-PI3K 27458163 29892225
Immunofluorescence Vimentin / Occludin ; IκBα / NF-κB p65 24508063 27212040
In vivo

Honokiol is highly effective against SVR angiosarcoma in nude mice. [1] Honokiol inhibits the growth of RKO cells in murine xenografts. [4] Honokiol prevents the growth of MDA-MD-231 breast cancer cells in murine xenografts. [7]

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 53 mg/mL
(198.99 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+40% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.

2mg/mL

Chemical Information

Molecular Weight 266.334
Formula

C18H18O2

CAS No. 35354-74-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C=CCC1=CC(=C(C=C1)O)C2=CC(=C(C=C2)O)CC=C

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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